Restriction Fragments for TIF1/YKR059W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

TIF1/YKR059W on chromosome XI from coordinates 554987 to 556174.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.7574.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 4 8 + Hpy188I TCNGA 6 5 . . 5 10 + Eco57MI CTGRAG 26 24 . . 5 10 + Eco57I CTGAAG 26 24 . . 9 12 + SetI ASST 12 8 . . 25 29 + MboII GAAGA 37 36 . . 28 32 + TfiI GAWTC 28 31 . . 28 32 + HinfI GANTC 28 31 . . 35 40 + ApoI RAATTY 35 39 . . 36 39 + TspEI AATT 35 39 . . 52 63 + DrdI GACNNNNNNGTC 58 56 . . 56 59 + SetI ASST 59 55 . . 61 66 + Hpy166II GTNNAC 63 63 . . 61 66 + AccI GTMKAC 62 64 . . 71 74 + TaqI TCGA 71 73 . . 83 86 + TspEI AATT 82 86 . . 100 103 + MseI TTAA 100 102 . . 105 108 + SetI ASST 108 104 . . 104 107 - MnlI CCTC 97 96 . . 116 120 + MaeIII GTNAC 115 120 . . 119 123 + Tsp4CI* ACNGT 121 120 . . 124 129 + AsuII TTCGAA 125 127 . . 125 128 + TaqI TCGA 125 127 . . 127 131 + MboII GAAGA 139 138 . . 133 137 + BccI CCATC 141 142 . . 150 153 + TaiI ACGT 153 149 . . 150 153 + SetI ASST 153 149 . . 150 153 + MaeII ACGT 150 152 . . 155 159 + BccI CCATC 163 164 . . 159 162 + FatI CATG 158 162 . . 159 162 + NlaIII CATG 162 158 . . 159 162 + CviAII CATG 159 161 . . 174 177 + SetI ASST 177 173 . . 176 180 + Tsp45I GTSAC 175 180 . . 176 180 + MaeIII GTNAC 175 180 . . 172 176 - Hin4II* CCTTC 166 165 . . 177 182 + Hpy178III* TCNNGA 178 180 . . 189 192 + CviJI RGCY 190 190 . . 191 196 + SmlI CTYRAG 191 195 . . 195 198 + SetI ASST 198 194 . . 195 198 + CviJI RGCY 196 196 . . 195 198 + AluI AGCT 196 196 . . 191 196 - Bce83I* CTTGAG 176 174 . . 206 209 + Csp6I GTAC 206 208 . . 206 209 + RsaI GTAC 207 207 . . 208 212 + BsrI ACTGG 213 211 . . 217 222 + Cfr10I RCCGGY 217 221 . . 217 222 + BetI* WCCGGW 217 221 . . 217 222 + AgeI ACCGGT 217 221 . . 218 221 + HpaII CCGG 218 220 . . 220 225 + KpnI GGTACC 224 220 . . 220 225 + NlaIV GGNNCC 222 222 . . 220 225 + HgiCI* GGYRCC 220 224 . . 220 225 + Acc65I GGTACC 220 224 . . 221 224 + Csp6I GTAC 221 223 . . 221 224 + RsaI GTAC 222 222 . . 223 226 + SetI ASST 226 222 . . 235 239 + BlsI GCNGC 237 236 . . 235 239 + BisI GCNGC 236 237 . . 235 239 + TseI GCWGC 235 238 . . 231 236 - BsrDI GCAATG 230 228 . . 235 239 - BbvI GCAGC 222 226 . . 242 245 + CviRI* TGCA 243 243 . . 249 252 + TspEI AATT 248 252 . . 253 264 + DrdI GACNNNNNNGTC 259 257 . . 256 259 + SetI ASST 259 255 . . 257 260 + MnlI CCTC 267 266 . . 267 270 + CviJI RGCY 268 268 . . 267 272 + NlaIV GGNNCC 269 269 . . 268 278 + MwoI GCNNNNNNNGC 274 271 . . 271 274 + MnlI CCTC 281 280 . . 272 277 + SmlI CTYRAG 272 276 . . 269 274 - BseRI GAGGAG 260 258 . . 275 280 + HindIII AAGCTT 275 279 . . 276 279 + SetI ASST 279 275 . . 276 279 + CviJI RGCY 277 277 . . 276 279 + AluI AGCT 277 277 . . 272 277 - Bce83I* CTTGAG 257 255 . . 282 292 + Hin4I GAYNNNNNVTC 273 268 305 300 288 291 + CviJI RGCY 289 289 . . 288 293 + NlaIV GGNNCC 290 290 . . 291 296 + MmeI TCCRAC 316 314 . . 296 299 + MaeI CTAG 296 298 . . 302 305 + TspEI AATT 301 305 . . 306 309 + CviJI RGCY 307 307 . . 311 314 + CviRI* TGCA 312 312 . . 323 326 + SetI ASST 326 322 . . 330 333 + FatI CATG 329 333 . . 330 333 + NlaIII CATG 333 329 . . 330 333 + CviAII CATG 330 332 . . 333 336 + CviJI RGCY 334 334 . . 339 342 + CviJI RGCY 340 340 . . 348 351 + FatI CATG 347 351 . . 348 351 + NlaIII CATG 351 347 . . 348 351 + CviAII CATG 348 350 . . 352 363 + DrdI GACNNNNNNGTC 358 356 . . 359 362 + SetI ASST 362 358 . . 360 364 + BmgT120I GGNCC 361 362 . . 360 364 + AvaII GGWCC 360 363 . . 360 364 + AsuI* GGNCC 360 363 . . 361 366 + Hpy166II GTNNAC 363 363 . . 365 375 + BaeI ACNNNNGTAYC 354 349 387 382 380 383 + Csp6I GTAC 380 382 . . 380 383 + RsaI GTAC 381 381 . . 394 398 + MboII GAAGA 406 405 . . 394 399 + BbvII* GAAGAC 401 405 . . 397 401 + HgaI GACGC 406 411 . . 401 406 + Eco57MI CTGRAG 422 420 . . 401 406 + Eco57I CTGAAG 422 420 . . 405 408 + SetI ASST 408 404 . . 403 407 - Hin4II* CCTTC 397 396 . . 411 421 + BplI GAGNNNNNCTC 402 397 434 429 415 419 - SfaNI GCATC 405 409 . . 411 421 - BplI GAGNNNNNCTC 434 429 402 397 426 430 + Hpy99I CGWCG 430 425 . . 434 437 + Csp6I GTAC 434 436 . . 434 437 + RsaI GTAC 435 435 . . 439 443 + BssKI CCNGG 438 443 . . 439 443 + BseBI CCWGG 440 441 . . 439 443 + ScrFI CCNGG 440 441 . . 439 443 + EcoRII CCWGG 438 443 . . 441 444 + SetI ASST 444 440 . . 437 442 - GsuI CTGGAG 422 420 . . 459 463 - FokI GGATG 445 449 . . 459 463 - BseGI GGATG 458 456 . . 468 471 + TaiI ACGT 471 467 . . 468 471 + SetI ASST 471 467 . . 468 471 + MaeII ACGT 468 470 . . 473 477 + TfiI GAWTC 473 476 . . 473 477 + HinfI GANTC 473 476 . . 476 480 + Hpy188I TCNGA 478 477 . . 489 492 + DpnI GATC 490 490 . . 489 492 + BstKTI GATC 491 489 . . 489 492 + MboI GATC 488 492 . . 491 496 + Hpy178III* TCNNGA 492 494 . . 495 504 + BsaBI GATNNNNATC 499 499 . . 499 503 - TspDTI ATGAA 489 487 . . 504 508 + DdeI CTNAG 504 507 . . 509 513 + TspDTI ATGAA 524 522 . . 513 516 + SetI ASST 516 512 . . 513 516 + CviJI RGCY 514 514 . . 513 516 + AluI AGCT 514 514 . . 518 522 + TspDTI ATGAA 533 531 . . 529 533 - MboII GAAGA 521 520 . . 528 533 - BbvII* GAAGAC 521 525 . . 557 562 + ApoI RAATTY 557 561 . . 558 561 + TspEI AATT 557 561 . . 564 575 + TstI CACNNNNNNTCC 555 550 587 582 565 568 + SetI ASST 568 564 . . 563 567 - HphI GGTGA 555 554 . . 584 589 + SmlI CTYRAG 584 588 . . 584 589 - Bce83I* CTTGAG 569 567 . . 581 592 - CspCI CAANNNNNGTGG 605 603 570 568 602 605 + AciI CCGC 602 604 . . 601 606 - EciI GGCGGA 591 589 . . 609 612 + FatI CATG 608 612 . . 609 612 + NlaIII CATG 612 608 . . 609 612 + CviAII CATG 609 611 . . 619 624 + ZraI GACGTC 621 621 . . 619 624 + AcyI GRCGYC 620 622 . . 619 624 + AatII GACGTC 623 619 . . 620 623 + TaiI ACGT 623 619 . . 620 623 + SetI ASST 623 619 . . 620 623 + MaeII ACGT 620 622 . . 631 635 + MaeIII GTNAC 630 635 . . 640 645 + ApoI RAATTY 640 644 . . 641 644 + TspEI AATT 640 644 . . 655 659 - BsrI ACTGG 655 653 . . 654 659 - BfiI ACTGGG 649 648 . . 662 667 + ApoI RAATTY 662 666 . . 663 666 + TspEI AATT 662 666 . . 671 674 + MseI TTAA 671 673 . . 668 673 - TsoI TARCCA 658 656 . . 678 682 + FokI GGATG 691 695 . . 678 682 + BseGI GGATG 684 682 . . 675 679 - Hin4II* CCTTC 669 668 . . 680 684 + TspDTI ATGAA 695 693 . . 683 686 + TspEI AATT 682 686 . . 696 699 + SetI ASST 699 695 . . 694 698 - Hin4II* CCTTC 688 687 . . 707 710 + TspEI AATT 706 710 . . 713 716 + TaiI ACGT 716 712 . . 713 716 + SetI ASST 716 712 . . 713 716 + MaeII ACGT 713 715 . . 716 719 + MseI TTAA 716 718 . . 724 728 + MboII GAAGA 736 735 . . 727 731 + MboII GAAGA 739 738 . . 730 734 + MboII GAAGA 742 741 . . 753 758 + TaqII GACCGA 769 767 . . 766 770 + HinfI GANTC 766 769 . . 766 776 + Hin4I GAYNNNNNVTC 757 752 789 784 767 777 + BsaXI ACNNNNNCTCC 757 754 787 784 766 770 - PleI GAGTC 760 761 . . 766 770 - MlyI GAGTC 760 760 . . 778 782 + MaeIII GTNAC 777 782 . . 775 779 - TspGWI ACGGA 765 763 . . 782 787 + SmlI CTYRAG 782 786 . . 786 789 + SetI ASST 789 785 . . 786 789 + CviJI RGCY 787 787 . . 786 789 + AluI AGCT 787 787 . . 782 787 - Bce83I* CTTGAG 767 765 . . 794 798 - MboII GAAGA 786 785 . . 800 804 + MaeIII GTNAC 799 804 . . 809 813 + MboII GAAGA 821 820 . . 815 818 + SetI ASST 818 814 . . 818 821 + TaqI TCGA 818 820 . . 820 824 + MboII GAAGA 832 831 . . 824 827 + TspEI AATT 823 827 . . 833 837 + DdeI CTNAG 833 836 . . 838 841 + MseI TTAA 838 840 . . 850 855 + ApoI RAATTY 850 854 . . 851 854 + TspEI AATT 850 854 . . 856 860 + Tsp4CI* ACNGT 858 857 . . 866 870 + BccI CCATC 874 875 . . 874 878 + Hpy188I TCNGA 876 875 . . 900 909 + MslI CAYNNNNRTG 904 904 . . 896 900 - BsmAI GTCTC 890 894 . . 902 906 + BccI CCATC 910 911 . . 905 910 + Hpy178III* TCNNGA 906 908 . . 905 910 + BspHI TCATGA 905 909 . . 896 906 - Hin4I GAYNNNNNVTC 919 914 887 882 906 909 + FatI CATG 905 909 . . 906 909 + NlaIII CATG 909 905 . . 906 909 + CviAII CATG 906 908 . . 907 911 + TspDTI ATGAA 922 920 . . 908 919 + BdaI TGANNNNNNTCA 897 895 931 929 909 918 + XmnI GAANNNNTTC 913 913 . . 909 913 - Hin4II* CCTTC 903 902 . . 913 918 + EcoRI GAATTC 913 917 . . 913 918 + ApoI RAATTY 913 917 . . 914 917 + TspEI AATT 913 917 . . 917 921 + Hpy188I TCNGA 919 918 . . 908 919 - BdaI TGANNNNNNTCA 931 929 897 895 920 929 + XmnI GAANNNNTTC 924 924 . . 921 931 + FalI AAGNNNNNCTT 912 907 944 939 931 936 + Hpy178III* TCNNGA 932 934 . . 921 931 - FalI AAGNNNNNCTT 944 939 912 907 928 932 - MboII GAAGA 920 919 . . 935 940 + ApoI RAATTY 935 939 . . 936 939 + TspEI AATT 935 939 . . 942 945 + DpnI GATC 943 943 . . 942 945 + BstKTI GATC 944 942 . . 942 945 + MboI GATC 941 945 . . 947 961 + XcmI CCANNNNNNNNNTGG 954 953 . . 948 954 + TspRI CASTGNN 954 945 . . 960 963 + CviJI RGCY 961 961 . . 962 965 + MaeI CTAG 962 964 . . 959 964 - TsoI TARCCA 949 947 . . 966 969 + SetI ASST 969 965 . . 965 968 - MnlI CCTC 958 957 . . 970 975 + ClaI ATCGAT 971 973 . . 971 974 + TaqI TCGA 971 973 . . 977 982 + MmeI TCCRAC 1002 1000 . . 992 997 - TsoI TARCCA 982 980 . . 998 1001 + MseI TTAA 998 1000 . . 1014 1017 + SetI ASST 1017 1013 . . 1014 1017 + CviJI RGCY 1015 1015 . . 1014 1017 + AluI AGCT 1015 1015 . . 1040 1044 + TfiI GAWTC 1040 1043 . . 1040 1044 + HinfI GANTC 1040 1043 . . 1050 1053 + SetI ASST 1053 1049 . . 1049 1052 - MnlI CCTC 1042 1041 . . 1079 1083 + BccI CCATC 1087 1088 . . 1090 1094 + MaeIII GTNAC 1089 1094 . . 1099 1103 + MboII GAAGA 1111 1110 . . 1099 1104 + BbvII* GAAGAC 1106 1110 . . 1103 1106 + TaiI ACGT 1106 1102 . . 1103 1106 + SetI ASST 1106 1102 . . 1103 1106 + MaeII ACGT 1103 1105 . . 1108 1113 + HaeII RGCGCY 1112 1108 . . 1109 1112 + Hin6I GCGC 1109 1111 . . 1109 1112 + GlaI GCGC 1110 1110 . . 1109 1112 + HhaI GCGC 1111 1109 . . 1123 1126 + MaeI CTAG 1123 1125 . . 1146 1149 + TspEI AATT 1145 1149 . . 1150 1154 + MboII GAAGA 1162 1161 . . 1139 1150 - CspCI CAANNNNNGTGG 1163 1161 1128 1126 1154 1157 + TspEI AATT 1153 1157 . . 1159 1163 + BccI CCATC 1167 1168 . . 1162 1167 + MmeI TCCRAC 1187 1185 . . 1162 1166 + Hpy188I TCNGA 1164 1163 . . 1160 1164 - FokI GGATG 1146 1150 . . 1160 1164 - BseGI GGATG 1159 1157 . . 1167 1172 - BtgZI GCGATG 1152 1156 . . #--------------------------------------- # # # AatII: # [GACGTC] # 565 623 # # Acc65I: # [GGTACC] # 220 968 # # AccI: # [GTMKAC] # 62 1126 # # AciI: # [CCGC] # 586 602 # # AcyI: # [GRCGYC] # 568 620 # # AgeI: # [ACCGGT] # 217 971 # # AluI: # [AGCT] # 81 173 196 228 237 273 # # ApoI: # [RAATTY] # 22 22 35 63 83 188 # 253 522 # # AsuI*: # [GGNCC] # 360 828 # # AsuII: # [TTCGAA] # 125 1063 # # AvaII: # [GGWCC] # 360 828 # # BaeI: # [ACNNNNGTAYC] # 33 354 801 # # BbvI: # [GCAGC] # 222 966 # # BbvII*: # [GAAGAC] # 82 120 401 585 # # BccI: # [CCATC] # 21 22 36 80 141 177 # 711 # # Bce83I*: # [CTTGAG] # 81 176 198 312 421 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 257 897 # # BetI*: # [WCCGGW] # 217 971 # # BfiI: # [ACTGGG] # 539 649 # # BisI: # [GCNGC] # 236 952 # # BlsI: # [GCNGC] # 237 951 # # BmgT120I: # [GGNCC] # 361 827 # # BplI: # [GAGNNNNNCTC] # 0 0 32 402 754 # # BsaBI: # [GATNNNNATC] # 499 689 # # BsaXI: # [ACNNNNNCTCC] # 30 401 757 # # BseBI: # [CCWGG] # 440 748 # # BseGI: # [GGATG] # 29 226 458 475 # # BseRI: # [GAGGAG] # 260 928 # # BsmAI: # [GTCTC] # 298 890 # # BspHI: # [TCATGA] # 283 905 # # BsrDI: # [GCAATG] # 230 958 # # BsrI: # [ACTGG] # 213 442 533 # # BssKI: # [CCNGG] # 438 750 # # BstKTI: # [GATC] # 244 453 491 # # BtgZI: # [GCGATG] # 36 1152 # # Cfr10I: # [RCCGGY] # 217 971 # # ClaI: # [ATCGAT] # 217 971 # # Csp6I: # [GTAC] # 15 54 159 206 754 # # CspCI: # [CAANNNNNGTGG] # 25 35 35 523 570 # # CviAII: # [CATG] # 18 159 171 261 282 297 # # CviJI: # [RGCY] # 6 6 9 12 18 27 # 54 72 173 174 174 190 # 273 # # CviRI*: # [TGCA] # 69 243 876 # # DdeI: # [CTNAG] # 329 355 504 # # DpnI: # [GATC] # 245 453 490 # # DrdI: # [GACNNNNNNGTC] # 58 99 201 830 # # EciI: # [GGCGGA] # 591 597 # # Eco57I: # [CTGAAG] # 26 396 766 # # Eco57MI: # [CTGRAG] # 26 396 766 # # EcoRI: # [GAATTC] # 275 913 # # EcoRII: # [CCWGG] # 438 750 # # FalI: # [AAGNNNNNCTT] # 0 0 32 244 912 # # FatI: # [CATG] # 18 158 171 261 283 297 # # FokI: # [GGATG] # 42 246 445 455 # # GlaI: # [GCGC] # 78 1110 # # GsuI: # [CTGGAG] # 422 766 # # HaeII: # [RGCGCY] # 76 1112 # # HgaI: # [GACGC] # 406 782 # # HgiCI*: # [GGYRCC] # 220 968 # # HhaI: # [GCGC] # 77 1111 # # Hin4I: # [GAYNNNNNVTC] # 32 32 32 98 269 273 # 452 # # Hin4II*: # [CCTTC] # 19 166 215 231 272 285 # # Hin6I: # [GCGC] # 79 1109 # # HindIII: # [AAGCTT] # 275 913 # # HinfI: # [GANTC] # 28 148 274 293 445 # # HpaII: # [CCGG] # 218 970 # # HphI: # [GGTGA] # 555 633 # # Hpy166II: # [GTNNAC] # 63 300 825 # # Hpy178III*: # [TCNNGA] # 26 178 256 314 414 # # Hpy188I: # [TCNGA] # 6 24 43 245 398 472 # # Hpy99I: # [CGWCG] # 430 758 # # KpnI: # [GGTACC] # 224 964 # # MaeI: # [CTAG] # 65 161 296 666 # # MaeII: # [ACGT] # 85 93 150 152 318 390 # # MaeIII: # [GTNAC] # 22 60 99 115 147 290 # 455 # # MboI: # [GATC] # 247 453 488 # # MboII: # [GAAGA] # 3 3 11 26 35 37 # 44 51 88 102 115 191 # 215 267 # # MlyI: # [GAGTC] # 428 760 # # MmeI: # [TCCRAC] # 1 185 316 686 # # MnlI: # [CCTC] # 14 84 97 146 170 677 # # MseI: # [TTAA] # 45 100 122 160 190 571 # # MslI: # [CAYNNNNRTG] # 284 904 # # MwoI: # [GCNNNNNNNGC] # 274 914 # # NlaIII: # [CATG] # 18 162 171 261 279 297 # # NlaIV: # [GGNNCC] # 21 47 222 898 # # PleI: # [GAGTC] # 428 760 # # RsaI: # [GTAC] # 15 54 159 207 753 # # ScrFI: # [CCNGG] # 440 748 # # SetI: # [ASST] # 12 17 20 21 24 27 # 28 29 33 36 36 36 # 45 45 46 47 47 48 # 49 52 53 55 73 76 # 82 151 # # SfaNI: # [GCATC] # 405 783 # # SmlI: # [CTYRAG] # 81 191 198 312 406 # # TaiI: # [ACGT] # 82 93 152 153 318 390 # # TaqI: # [TCGA] # 54 71 153 217 693 # # TaqII: # [GACCGA] # 419 769 # # TfiI: # [GAWTC] # 28 148 445 567 # # TseI: # [GCWGC] # 235 953 # # TsoI: # [TARCCA] # 33 206 291 658 # # Tsp45I: # [GTSAC] # 175 1013 # # Tsp4CI*: # [ACNGT] # 121 330 737 # # TspDTI: # [ATGAA] # 9 35 162 227 266 489 # # TspEI: # [AATT] # 8 20 22 22 24 27 # 35 35 47 53 63 83 # 117 166 210 256 # # TspGWI: # [ACGGA] # 423 765 # # TspRI: # [CASTGNN] # 234 954 # # TstI: # [CACNNNNNNTCC] # 32 555 601 # # XcmI: # [CCANNNNNNNNNTGG] # 234 954 # # XmnI: # [GAANNNNTTC] # 11 264 913 # # ZraI: # [GACGTC] # 567 621 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1188 # Reported_sequences: 1 # Reported_hitcount: 283 #---------------------------------------