Restriction Fragments for YKLCsigma1



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The currently selected gene/sequence is :

YKLCsigma1 on chromosome XI from coordinates 202983 to 202644.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.10480.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 16 27 + BdaI TGANNNNNNTCA 5 3 39 37 16 27 - BdaI TGANNNNNNTCA 39 37 5 3 32 42 + Hin4I GAYNNNNNVTC 23 18 55 50 32 42 + Eam1105I GACNNNNNGTC 37 36 . . 38 41 + TaiI ACGT 41 37 . . 38 41 + SetI ASST 41 37 . . 38 41 + MaeII ACGT 38 40 . . 40 44 + Tsp45I GTSAC 39 44 . . 40 44 + MaeIII GTNAC 39 44 . . 32 42 - Hin4I GAYNNNNNVTC 55 50 23 18 41 45 - HphI GGTGA 33 32 . . 49 54 + AclI AACGTT 50 52 . . 50 53 + TaiI ACGT 53 49 . . 50 53 + SetI ASST 53 49 . . 50 53 + MaeII ACGT 50 52 . . 53 57 - TspDTI ATGAA 43 41 . . 64 69 + NdeI CATATG 65 67 . . 67 71 + TspDTI ATGAA 82 80 . . 75 78 + SetI ASST 78 74 . . 78 83 + PsiI TTATAA 80 80 . . 96 99 + FatI CATG 95 99 . . 96 99 + NlaIII CATG 99 95 . . 96 99 + CviAII CATG 96 98 . . 99 103 - BsmAI GTCTC 93 97 . . 112 115 + SetI ASST 115 111 . . 113 117 + Hin4II* CCTTC 123 122 . . 119 122 + MaeI CTAG 119 121 . . 121 124 + SetI ASST 124 120 . . 121 124 + CviJI RGCY 122 122 . . 121 124 + AluI AGCT 122 122 . . 153 156 + TspEI AATT 152 156 . . 162 167 + XhoII RGATCY 162 166 . . 163 166 + DpnI GATC 164 164 . . 163 166 + BstKTI GATC 165 163 . . 163 166 + MboI GATC 162 166 . . 165 169 + Hpy188I TCNGA 167 166 . . 163 167 - BinI* GGATC 157 158 . . 170 174 + TfiI GAWTC 170 173 . . 170 174 + HinfI GANTC 170 173 . . 174 177 + AciI CCGC 174 176 . . 175 178 + FnuDII* CGCG 176 176 . . 176 179 + Hin6I GCGC 176 178 . . 176 179 + GlaI GCGC 177 177 . . 176 179 + HhaI GCGC 178 176 . . 188 192 + DdeI CTNAG 188 191 . . 196 200 + TfiI GAWTC 196 199 . . 196 200 + HinfI GANTC 196 199 . . 198 202 - TspDTI ATGAA 188 186 . . 226 230 + MaeIII GTNAC 225 230 . . 233 237 - TspGWI ACGGA 223 221 . . 239 242 + TspEI AATT 238 242 . . 240 245 + VspI ATTAAT 241 243 . . 241 244 + MseI TTAA 241 243 . . 245 249 + Hpy188I TCNGA 247 246 . . 253 257 + Tsp4CI* ACNGT 255 254 . . 265 269 + BsrI ACTGG 270 268 . . 269 273 + MaeIII GTNAC 268 273 . . 267 272 - TsoI TARCCA 257 255 . . 278 282 + DdeI CTNAG 278 281 . . 283 287 + Tsp4CI* ACNGT 285 284 . . 302 307 + Hpy178III* TCNNGA 303 305 . . 311 316 + BetI* WCCGGW 311 315 . . 312 315 + HpaII CCGG 312 314 . . 320 327 + PspXI VCTCGAGB 321 325 . . 321 326 + SmlI CTYRAG 321 325 . . 321 326 + XhoI CTCGAG 321 325 . . 321 326 + BmeT110I CYCGRG 322 324 . . 321 326 + AvaI CYCGRG 321 325 . . 322 325 + TaqI TCGA 322 324 . . 324 329 + SduI GDGCHC 328 324 . . 324 329 + HgiJII* GRGCYC 328 324 . . 325 328 + CviJI RGCY 326 326 . . #--------------------------------------- # # # AciI: # [CCGC] # 166 174 # # AclI: # [AACGTT] # 50 290 # # AluI: # [AGCT] # 122 218 # # AvaI: # [CYCGRG] # 19 321 # # BdaI: # [TGANNNNNNTCA] # 0 0 5 34 301 # # BetI*: # [WCCGGW] # 29 311 # # BinI*: # [GGATC] # 157 183 # # BmeT110I: # [CYCGRG] # 18 322 # # BsmAI: # [GTCTC] # 93 247 # # BsrI: # [ACTGG] # 70 270 # # BstKTI: # [GATC] # 165 175 # # CviAII: # [CATG] # 96 244 # # CviJI: # [RGCY] # 14 122 204 # # DdeI: # [CTNAG] # 62 90 188 # # DpnI: # [GATC] # 164 176 # # Eam1105I: # [GACNNNNNGTC] # 37 303 # # FatI: # [CATG] # 95 245 # # FnuDII*: # [CGCG] # 164 176 # # GlaI: # [GCGC] # 163 177 # # HgiJII*: # [GRGCYC] # 12 328 # # HhaI: # [GCGC] # 162 178 # # Hin4I: # [GAYNNNNNVTC] # 0 0 23 32 285 # # Hin4II*: # [CCTTC] # 123 217 # # Hin6I: # [GCGC] # 164 176 # # HinfI: # [GANTC] # 26 144 170 # # HpaII: # [CCGG] # 28 312 # # HphI: # [GGTGA] # 33 307 # # Hpy178III*: # [TCNNGA] # 37 303 # # Hpy188I: # [TCNGA] # 80 93 167 # # MaeI: # [CTAG] # 119 221 # # MaeII: # [ACGT] # 12 38 290 # # MaeIII: # [GTNAC] # 39 43 72 186 # # MboI: # [GATC] # 162 178 # # MseI: # [TTAA] # 99 241 # # NdeI: # [CATATG] # 65 275 # # NlaIII: # [CATG] # 99 241 # # PsiI: # [TTATAA] # 80 260 # # PspXI: # [VCTCGAGB] # 19 321 # # SduI: # [GDGCHC] # 12 328 # # SetI: # [ASST] # 9 12 25 37 41 216 # # SmlI: # [CTYRAG] # 19 321 # # TaiI: # [ACGT] # 12 41 287 # # TaqI: # [TCGA] # 18 322 # # TfiI: # [GAWTC] # 26 144 170 # # TsoI: # [TARCCA] # 83 257 # # Tsp45I: # [GTSAC] # 39 301 # # Tsp4CI*: # [ACNGT] # 30 55 255 # # TspDTI: # [ATGAA] # 39 43 106 152 # # TspEI: # [AATT] # 86 102 152 # # TspGWI: # [ACGGA] # 117 223 # # VspI: # [ATTAAT] # 99 241 # # XhoI: # [CTCGAG] # 19 321 # # XhoII: # [RGATCY] # 162 178 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 340 # Reported_sequences: 1 # Reported_hitcount: 72 #---------------------------------------