Restriction Fragments for URA1/YKL216W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

URA1/YKL216W on chromosome XI from coordinates 25215 to 26159.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.5812.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 6 9 + CviJI RGCY 7 7 . . 8 12 - BsrI ACTGG 8 6 . . 13 16 + MseI TTAA 13 15 . . 26 31 + Hpy178III* TCNNGA 27 29 . . 37 40 + SetI ASST 40 36 . . 41 45 + TspDTI ATGAA 56 54 . . 55 59 + TspDTI ATGAA 70 68 . . 57 62 + BsmI GAATGC 63 61 . . 59 64 + EcoT22I ATGCAT 63 59 . . 60 63 + CviRI* TGCA 61 61 . . 61 65 + SfaNI GCATC 70 74 . . 64 69 + BetI* WCCGGW 64 68 . . 65 68 + HpaII CCGG 65 67 . . 62 66 - FokI GGATG 48 52 . . 62 66 - BseGI GGATG 61 59 . . 71 75 - TspDTI ATGAA 61 59 . . 76 79 + CviRI* TGCA 77 77 . . 78 81 + FatI CATG 77 81 . . 78 81 + NlaIII CATG 81 77 . . 78 81 + CviAII CATG 78 80 . . 73 78 - BsrDI GCAATG 72 70 . . 92 95 + TspEI AATT 91 95 . . 98 102 + TspDTI ATGAA 113 111 . . 101 104 + TspEI AATT 100 104 . . 117 120 + SetI ASST 120 116 . . 117 120 + CviJI RGCY 118 118 . . 117 120 + AluI AGCT 118 118 . . 118 123 + Cac8I GCNNGC 120 120 . . 122 125 + Hin6I GCGC 122 124 . . 122 125 + GlaI GCGC 123 123 . . 122 125 + HhaI GCGC 124 122 . . 124 129 - BsmI GAATGC 124 122 . . 127 131 - TspDTI ATGAA 117 115 . . 148 151 + SetI ASST 151 147 . . 150 154 + DdeI CTNAG 150 153 . . 162 165 + SetI ASST 165 161 . . 163 169 + BstEII GGTNACC 163 168 . . 164 168 + MaeIII GTNAC 163 168 . . 160 164 - Hin4II* CCTTC 154 153 . . 177 180 + TaiI ACGT 180 176 . . 177 180 + SetI ASST 180 176 . . 177 180 + MaeII ACGT 177 179 . . 179 183 + MaeIII GTNAC 178 183 . . 193 196 + MnlI CCTC 203 202 . . 190 194 - BslFI GGGAC 175 179 . . 196 199 + MaeI CTAG 196 198 . . 212 217 + DsaI* CCRYGG 212 216 . . 212 217 + StyI CCWWGG 212 216 . . 212 217 + NcoI CCATGG 212 216 . . 212 217 + SecI* CCNNGG 212 216 . . 213 216 + FatI CATG 212 216 . . 213 216 + NlaIII CATG 216 212 . . 213 216 + CviAII CATG 213 215 . . 218 223 + Hpy166II GTNNAC 220 220 . . 223 233 + BsiYI* CCNNNNNNNGG 229 226 . . 227 237 + BaeI ACNNNNGTAYC 216 211 249 244 231 234 + SetI ASST 234 230 . . 229 233 - Hin4II* CCTTC 223 222 . . 236 239 + TaqI TCGA 236 238 . . 259 262 + MseI TTAA 259 261 . . 263 267 + Tsp4CI* ACNGT 265 264 . . 274 277 + TspEI AATT 273 277 . . 279 284 + Hpy178III* TCNNGA 280 282 . . 285 288 + CviRI* TGCA 286 286 . . 283 287 - SfaNI GCATC 273 277 . . 287 293 + AarI CACCTGC 297 301 . . 288 291 + SetI ASST 291 287 . . 288 293 + BspMI ACCTGC 297 301 . . 299 303 - MboII GAAGA 291 290 . . 303 307 + DdeI CTNAG 303 306 . . 303 307 + BspCNI CTCAG 316 314 . . 303 307 + BseMII CTCAG 317 315 . . 305 313 + AlwNI CAGNNNCTG 310 307 . . 326 330 + TspDTI ATGAA 341 339 . . 330 335 + ApoI RAATTY 330 334 . . 331 334 + TspEI AATT 330 334 . . 332 339 + SwaI ATTTAAAT 335 335 . . 333 338 + AhaIII* TTTAAA 335 335 . . 334 337 + MseI TTAA 334 336 . . 336 341 + ApoI RAATTY 336 340 . . 337 340 + TspEI AATT 336 340 . . 345 348 - MnlI CCTC 338 337 . . 364 369 + EcoRI GAATTC 364 368 . . 364 369 + ApoI RAATTY 364 368 . . 365 368 + TspEI AATT 364 368 . . 371 375 + Tsp4CI* ACNGT 373 372 . . 385 388 + MseI TTAA 385 387 . . 409 413 + BssKI CCNGG 408 413 . . 409 413 + BseBI CCWGG 410 411 . . 409 414 + SecI* CCNNGG 409 413 . . 409 413 + ScrFI CCNGG 410 411 . . 409 413 + EcoRII CCWGG 408 413 . . 448 458 + FalI AAGNNNNNCTT 439 434 471 466 454 457 + SetI ASST 457 453 . . 455 460 + StyI CCWWGG 455 459 . . 455 460 + SecI* CCNNGG 455 459 . . 448 458 - FalI AAGNNNNNCTT 471 466 439 434 464 467 + SetI ASST 467 463 . . 486 489 + SetI ASST 489 485 . . 487 490 + MnlI CCTC 497 496 . . 505 508 + MnlI CCTC 515 514 . . 532 537 + EcoRV GATATC 534 534 . . 537 540 + FatI CATG 536 540 . . 537 540 + NlaIII CATG 540 536 . . 537 540 + CviAII CATG 537 539 . . 546 551 + SspI AATATT 548 548 . . 552 561 + XmnI GAANNNNTTC 556 556 . . 567 570 + SetI ASST 570 566 . . 567 570 + CviJI RGCY 568 568 . . 567 570 + AluI AGCT 568 568 . . 574 579 + Hpy166II GTNNAC 576 576 . . 574 579 + HindII GTYRAC 576 576 . . 609 618 + MslI CAYNNNNRTG 613 613 . . 607 611 - TspDTI ATGAA 597 595 . . 621 625 - Hin4II* CCTTC 615 614 . . 641 644 + CviJI RGCY 642 642 . . 643 653 + BsiYI* CCNNNNNNNGG 649 646 . . 643 653 + PflMI CCANNNNNTGG 649 646 . . 648 657 + XmnI GAANNNNTTC 652 652 . . 669 672 + SetI ASST 672 668 . . 670 674 + HphI GGTGA 682 681 . . 668 671 - MnlI CCTC 661 660 . . 680 683 + MseI TTAA 680 682 . . 683 686 + CviJI RGCY 684 684 . . 684 694 + MwoI GCNNNNNNNGC 690 687 . . 689 692 + AciI CCGC 689 691 . . 690 693 + FnuDII* CGCG 691 691 . . 691 694 + Hin6I GCGC 691 693 . . 691 694 + GlaI GCGC 692 692 . . 691 694 + HhaI GCGC 693 691 . . 693 698 + Cac8I GCNNGC 695 695 . . 708 711 + CviRI* TGCA 709 709 . . 728 738 + Hin4I GAYNNNNNVTC 719 714 751 746 729 732 + SetI ASST 732 728 . . 726 730 - BsmAI GTCTC 720 724 . . 726 731 - Eco31I GGTCTC 720 724 . . 728 738 - Hin4I GAYNNNNNVTC 751 746 719 714 749 752 + Csp6I GTAC 749 751 . . 749 752 + RsaI GTAC 750 750 . . 753 756 + SetI ASST 756 752 . . 759 762 + TspEI AATT 758 762 . . 761 764 + MseI TTAA 761 763 . . 766 771 + BetI* WCCGGW 766 770 . . 767 770 + HpaII CCGG 767 769 . . 774 778 + FokI GGATG 787 791 . . 774 778 + BseGI GGATG 780 778 . . 776 781 + EcoT22I ATGCAT 780 776 . . 777 780 + CviRI* TGCA 778 778 . . 775 779 - SfaNI GCATC 765 769 . . 784 793 + XmnI GAANNNNTTC 788 788 . . 789 793 - MboII GAAGA 781 780 . . 799 804 + NlaIV GGNNCC 801 801 . . 799 804 + HgiCI* GGYRCC 799 803 . . 803 806 + MnlI CCTC 813 812 . . 811 816 + SfeI* CTRYAG 811 815 . . 821 824 + Csp6I GTAC 821 823 . . 821 824 + RsaI GTAC 822 822 . . 827 830 + TspEI AATT 826 830 . . 838 841 - MnlI CCTC 831 830 . . 841 846 + AcyI GRCGYC 842 844 . . 845 850 + Hpy178III* TCNNGA 846 848 . . 842 846 - HgaI GACGC 831 836 . . 858 861 + TaiI ACGT 861 857 . . 858 861 + SetI ASST 861 857 . . 858 861 + MaeII ACGT 858 860 . . 863 866 + TaqI TCGA 863 865 . . 872 875 + TspEI AATT 871 875 . . 874 877 + MseI TTAA 874 876 . . 891 894 + SetI ASST 894 890 . . 891 894 + CviJI RGCY 892 892 . . 891 894 + AluI AGCT 892 892 . . 893 897 + DdeI CTNAG 893 896 . . 892 898 - Bpu10I CCTNAGC 893 896 . . 905 909 - FokI GGATG 891 895 . . 905 909 - BseGI GGATG 904 902 . . 913 916 + DpnI GATC 914 914 . . 913 916 + BstKTI GATC 915 913 . . 913 916 + MboI GATC 912 916 . . 921 926 + DsaI* CCRYGG 921 925 . . 921 926 + SecI* CCNNGG 921 925 . . 920 924 - TspGWI ACGGA 910 908 . . 942 945 + MseI TTAA 942 944 . . #--------------------------------------- # # # AarI: # [CACCTGC] # 297 648 # # AciI: # [CCGC] # 256 689 # # AcyI: # [GRCGYC] # 103 842 # # AhaIII*: # [TTTAAA] # 335 610 # # AluI: # [AGCT] # 53 118 324 450 # # AlwNI: # [CAGNNNCTG] # 310 635 # # ApoI: # [RAATTY] # 6 28 330 581 # # BaeI: # [ACNNNNGTAYC] # 33 216 696 # # BetI*: # [WCCGGW] # 64 179 702 # # Bpu10I: # [CCTNAGC] # 52 893 # # BseBI: # [CCWGG] # 410 535 # # BseGI: # [GGATG] # 41 61 124 719 # # BseMII: # [CTCAG] # 317 628 # # BsiYI*: # [CCNNNNNNNGG] # 229 296 420 # # BslFI: # [GGGAC] # 175 770 # # BsmAI: # [GTCTC] # 225 720 # # BsmI: # [GAATGC] # 61 63 821 # # BspCNI: # [CTCAG] # 316 629 # # BspMI: # [ACCTGC] # 297 648 # # BsrDI: # [GCAATG] # 72 873 # # BsrI: # [ACTGG] # 8 937 # # BssKI: # [CCNGG] # 408 537 # # BstEII: # [GGTNACC] # 163 782 # # BstKTI: # [GATC] # 30 915 # # Cac8I: # [GCNNGC] # 120 250 575 # # Csp6I: # [GTAC] # 72 124 749 # # CviAII: # [CATG] # 78 135 324 408 # # CviJI: # [RGCY] # 7 42 53 74 111 208 # 450 # # CviRI*: # [TGCA] # 16 61 69 167 209 423 # # DdeI: # [CTNAG] # 52 150 153 590 # # DpnI: # [GATC] # 31 914 # # DsaI*: # [CCRYGG] # 24 212 709 # # Eco31I: # [GGTCTC] # 225 720 # # EcoRI: # [GAATTC] # 364 581 # # EcoRII: # [CCWGG] # 408 537 # # EcoRV: # [GATATC] # 411 534 # # EcoT22I: # [ATGCAT] # 63 165 717 # # FalI: # [AAGNNNNNCTT] # 0 0 32 439 474 # # FatI: # [CATG] # 77 135 324 409 # # FnuDII*: # [CGCG] # 254 691 # # FokI: # [GGATG] # 48 54 104 739 # # GlaI: # [GCGC] # 123 253 569 # # HgaI: # [GACGC] # 114 831 # # HgiCI*: # [GGYRCC] # 146 799 # # HhaI: # [GCGC] # 124 252 569 # # Hin4I: # [GAYNNNNNVTC] # 0 0 32 194 719 # # Hin4II*: # [CCTTC] # 69 154 330 392 # # Hin6I: # [GCGC] # 122 254 569 # # HindII: # [GTYRAC] # 369 576 # # HpaII: # [CCGG] # 65 178 702 # # HphI: # [GGTGA] # 263 682 # # Hpy166II: # [GTNNAC] # 220 356 369 # # Hpy178III*: # [TCNNGA] # 27 99 253 566 # # MaeI: # [CTAG] # 196 749 # # MaeII: # [ACGT] # 87 177 681 # # MaeIII: # [GTNAC] # 15 163 767 # # MboI: # [GATC] # 33 912 # # MboII: # [GAAGA] # 164 291 490 # # MnlI: # [CCTC] # 18 18 114 135 146 152 # 159 203 # # MseI: # [TTAA] # 3 13 51 68 75 81 # 113 246 295 # # MslI: # [CAYNNNNRTG] # 332 613 # # MwoI: # [GCNNNNNNNGC] # 255 690 # # NcoI: # [CCATGG] # 212 733 # # NlaIII: # [CATG] # 81 135 324 405 # # NlaIV: # [GGNNCC] # 144 801 # # PflMI: # [CCANNNNNTGG] # 296 649 # # RsaI: # [GTAC] # 72 123 750 # # ScrFI: # [CCNGG] # 410 535 # # SecI*: # [CCNNGG] # 24 46 197 212 466 # # SetI: # [ASST] # 10 14 15 22 24 31 # 33 40 51 54 57 60 # 80 81 102 105 166 # # SfaNI: # [GCATC] # 70 180 203 492 # # SfeI*: # [CTRYAG] # 134 811 # # SspI: # [AATATT] # 397 548 # # StyI: # [CCWWGG] # 212 243 490 # # SwaI: # [ATTTAAAT] # 335 610 # # TaiI: # [ACGT] # 84 180 681 # # TaqI: # [TCGA] # 82 236 627 # # Tsp4CI*: # [ACNGT] # 108 265 572 # # TspDTI: # [ATGAA] # 4 5 9 43 56 224 # 256 348 # # TspEI: # [AATT] # 6 9 28 45 57 68 # 74 91 173 394 # # TspGWI: # [ACGGA] # 35 910 # # XmnI: # [GAANNNNTTC] # 96 136 157 556 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 945 # Reported_sequences: 1 # Reported_hitcount: 182 #---------------------------------------