Restriction Fragments for MCD4/YKL165C



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The currently selected gene/sequence is :

MCD4/YKL165C on chromosome XI from coordinates 140691 to 137932.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.3733.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 20 24 + Hpy99I CGWCG 24 19 . . 21 25 + HgaI GACGC 30 35 . . 30 33 + CviJI RGCY 31 31 . . 47 51 - TspDTI ATGAA 37 35 . . 59 62 + SetI ASST 62 58 . . 76 81 + EcoRV GATATC 78 78 . . 85 89 - TspDTI ATGAA 75 73 . . 94 97 + AciI CCGC 94 96 . . 94 99 + BsrBI CCGCTC 96 96 . . 91 95 - HphI GGTGA 83 82 . . 103 106 + FatI CATG 102 106 . . 103 106 + NlaIII CATG 106 102 . . 103 106 + CviAII CATG 103 105 . . 101 105 - TspDTI ATGAA 91 89 . . 111 116 + SduI GDGCHC 115 111 . . 111 116 + HgiJII* GRGCYC 115 111 . . 112 115 + CviJI RGCY 113 113 . . 111 121 - Hin4I GAYNNNNNVTC 134 129 102 97 124 129 + TatI WGTACW 124 128 . . 124 129 + ScaI AGTACT 126 126 . . 125 128 + Csp6I GTAC 125 127 . . 125 128 + RsaI GTAC 126 126 . . 129 134 + MmeI TCCRAC 154 152 . . 136 139 + MnlI CCTC 146 145 . . 141 144 + CviRI* TGCA 142 142 . . 166 170 + HphI GGTGA 178 177 . . 173 178 + Hpy166II GTNNAC 175 175 . . 169 173 - BccI CCATC 163 164 . . 176 181 + BsaAI YACGTR 178 178 . . 177 180 + TaiI ACGT 180 176 . . 177 180 + SetI ASST 180 176 . . 177 180 + MaeII ACGT 177 179 . . 179 184 + BsgI GTGCAG 200 198 . . 180 183 + CviRI* TGCA 181 181 . . 202 206 + Tsp45I GTSAC 201 206 . . 202 206 + MaeIII GTNAC 201 206 . . 211 221 + BsiYI* CCNNNNNNNGG 217 214 . . 208 212 - FokI GGATG 194 198 . . 208 212 - BseGI GGATG 207 205 . . 214 219 + BciVI GTATCC 225 224 . . 211 215 - BsrI ACTGG 211 209 . . 217 222 + Hpy178III* TCNNGA 218 220 . . 217 222 + BetI* WCCGGW 217 221 . . 217 222 + BspMII* TCCGGA 217 221 . . 218 221 + HpaII CCGG 218 220 . . 229 234 + ApoI RAATTY 229 233 . . 230 233 + TspEI AATT 229 233 . . 237 242 + NlaIV GGNNCC 239 239 . . 237 242 + HgiCI* GGYRCC 237 241 . . 240 243 + SetI ASST 243 239 . . 250 255 + XhoII RGATCY 250 254 . . 250 255 + BglII AGATCT 250 254 . . 251 254 + DpnI GATC 252 252 . . 251 254 + BstKTI GATC 253 251 . . 251 254 + MboI GATC 250 254 . . 262 266 + TspDTI ATGAA 277 275 . . 274 277 + SetI ASST 277 273 . . 278 282 + Tsp4CI* ACNGT 280 279 . . 296 301 + BsmI GAATGC 302 300 . . 307 311 + TfiI GAWTC 307 310 . . 307 311 + HinfI GANTC 307 310 . . 316 320 + BssKI CCNGG 315 320 . . 316 320 + BseBI CCWGG 317 318 . . 316 320 + ScrFI CCNGG 317 318 . . 316 320 + EcoRII CCWGG 315 320 . . 322 325 + FatI CATG 321 325 . . 322 325 + NlaIII CATG 325 321 . . 322 325 + CviAII CATG 322 324 . . 328 338 + MwoI GCNNNNNNNGC 334 331 . . 337 342 - GsaI CCCAGC 341 337 . . 337 342 - BseYI CCCAGC 337 341 . . 349 353 + MboII GAAGA 361 360 . . 364 368 + Tsp45I GTSAC 363 368 . . 364 368 + MaeIII GTNAC 363 368 . . 361 365 - BceAI ACGGC 346 348 . . 372 375 + SetI ASST 375 371 . . 374 379 - MmeI TCCRAC 355 353 . . 394 397 + TspEI AATT 393 397 . . 398 401 + TaqI TCGA 398 400 . . 432 436 - TspDTI ATGAA 422 420 . . 440 445 + Hpy166II GTNNAC 442 442 . . 444 447 + SetI ASST 447 443 . . 442 446 - HphI GGTGA 434 433 . . 456 459 + SetI ASST 459 455 . . 472 477 + HaeII RGCGCY 476 472 . . 473 476 + Hin6I GCGC 473 475 . . 473 476 + GlaI GCGC 474 474 . . 469 473 - BccI CCATC 463 464 . . 473 476 + HhaI GCGC 475 473 . . 478 482 + Hpy188I TCNGA 480 479 . . 493 498 + Hpy166II GTNNAC 495 495 . . 493 498 + HindII GTYRAC 495 495 . . 498 507 + MslI CAYNNNNRTG 502 502 . . 503 507 + FokI GGATG 516 520 . . 503 507 + BseGI GGATG 509 507 . . 504 514 + Hin4I GAYNNNNNVTC 495 490 527 522 510 515 + BclI TGATCA 510 514 . . 511 514 + DpnI GATC 512 512 . . 511 514 + BstKTI GATC 513 511 . . 511 514 + MboI GATC 510 514 . . 504 514 - Hin4I GAYNNNNNVTC 527 522 495 490 521 524 + TaqI TCGA 521 523 . . 523 526 - MnlI CCTC 516 515 . . 538 542 - MboII GAAGA 530 529 . . 542 553 + BstXI CCANNNNNNTGG 549 545 . . 542 546 + BccI CCATC 550 551 . . 545 548 + TaqI TCGA 545 547 . . 546 557 + BcgI CGANNNNNNTGC 535 533 569 567 548 551 + SetI ASST 551 547 . . 548 551 + CviJI RGCY 549 549 . . 548 551 + AluI AGCT 549 549 . . 552 556 + FokI GGATG 565 569 . . 552 556 + BseGI GGATG 558 556 . . 553 557 - SfaNI GCATC 543 547 . . 576 580 + BinI* GGATC 584 585 . . 577 580 + DpnI GATC 578 578 . . 577 580 + BstKTI GATC 579 577 . . 577 580 + MboI GATC 576 580 . . 581 584 + TspEI AATT 580 584 . . 592 595 + TspEI AATT 591 595 . . 599 605 + TspRI CASTGNN 605 596 . . 603 616 + AjuI GAANNNNNNNTTGG 595 590 627 622 620 624 + TspDTI ATGAA 635 633 . . 624 627 + TspEI AATT 623 627 . . 635 639 + Tsp4CI* ACNGT 637 636 . . 661 664 + MaeI CTAG 661 663 . . 663 666 + SetI ASST 666 662 . . 677 680 + HpaII CCGG 677 679 . . 703 708 + NmeAIII GCCGAG 729 727 . . 715 723 + Tth111I GACNNNGTC 718 719 . . 735 740 + BclI TGATCA 735 739 . . 736 739 + DpnI GATC 737 737 . . 736 739 + BstKTI GATC 738 736 . . 736 739 + MboI GATC 735 739 . . 757 765 + Tth111I GACNNNGTC 760 761 . . 763 768 + Hpy166II GTNNAC 765 765 . . 763 768 + HindII GTYRAC 765 765 . . 778 781 - AciI CCGC 778 780 . . 791 794 + AciI CCGC 791 793 . . 806 811 + EcoP15I CAGCAG 836 838 . . 811 814 + DpnI GATC 812 812 . . 811 814 + BstKTI GATC 813 811 . . 811 814 + MboI GATC 810 814 . . 814 817 + FatI CATG 813 817 . . 814 817 + NlaIII CATG 817 813 . . 814 817 + CviAII CATG 814 816 . . 825 828 + CviRI* TGCA 826 826 . . 832 836 + BinI* GGATC 840 841 . . 833 836 + DpnI GATC 834 834 . . 833 836 + BstKTI GATC 835 833 . . 833 836 + MboI GATC 832 836 . . 836 844 + DraIII CACNNNGTG 841 838 . . 838 841 + FatI CATG 837 841 . . 838 841 + NlaIII CATG 841 837 . . 838 841 + CviAII CATG 838 840 . . 841 845 + HphI GGTGA 853 852 . . 842 846 + Tsp45I GTSAC 841 846 . . 842 846 + MaeIII GTNAC 841 846 . . 845 849 + Tsp4CI* ACNGT 847 846 . . 850 854 - FokI GGATG 836 840 . . 850 854 - BseGI GGATG 849 847 . . 865 871 + PpuMI RGGWCCY 866 869 . . 865 871 + DraII RGGNCCY 866 869 . . 866 871 + NlaIV GGNNCC 868 868 . . 866 870 + BmgT120I GGNCC 867 868 . . 866 870 + AvaII GGWCC 866 869 . . 866 870 + AsuI* GGNCC 866 869 . . 871 874 + MnlI CCTC 881 880 . . 880 890 + MwoI GCNNNNNNNGC 886 883 . . 889 892 - AciI CCGC 889 891 . . 889 893 - FauI CCCGC 882 884 . . 906 911 + TatI WGTACW 906 910 . . 907 910 + Csp6I GTAC 907 909 . . 907 910 + RsaI GTAC 908 908 . . 904 908 - BsrI ACTGG 904 902 . . 921 926 + BetI* WCCGGW 921 925 . . 922 925 + HpaII CCGG 922 924 . . 925 930 + BciVI GTATCC 936 935 . . 928 933 + MmeI TCCRAC 953 951 . . 928 932 + Hpy188I TCNGA 930 929 . . 946 949 + TspEI AATT 945 949 . . 953 956 + SetI ASST 956 952 . . 953 956 + CviJI RGCY 954 954 . . 953 956 + AluI AGCT 954 954 . . 958 961 + TaqI TCGA 958 960 . . 965 968 + MseI TTAA 965 967 . . 984 989 + Cac8I GCNNGC 986 986 . . 988 998 + MwoI GCNNNNNNNGC 994 991 . . 988 998 + BstAPI GCANNNNNTGC 994 991 . . 1003 1006 + MseI TTAA 1003 1005 . . 1024 1029 + Hpy166II GTNNAC 1026 1026 . . 1038 1043 + ApoI RAATTY 1038 1042 . . 1039 1042 + TspEI AATT 1038 1042 . . 1048 1052 + HphI GGTGA 1060 1059 . . 1053 1057 + MaeIII GTNAC 1052 1057 . . 1069 1074 + ClaI ATCGAT 1070 1072 . . 1070 1073 + TaqI TCGA 1070 1072 . . 1072 1076 - BccI CCATC 1066 1067 . . 1085 1089 + Tsp45I GTSAC 1084 1089 . . 1085 1089 + MaeIII GTNAC 1084 1089 . . 1090 1095 + HindIII AAGCTT 1090 1094 . . 1091 1094 + SetI ASST 1094 1090 . . 1091 1094 + CviJI RGCY 1092 1092 . . 1091 1094 + AluI AGCT 1092 1092 . . 1092 1097 + Cac8I GCNNGC 1094 1094 . . 1096 1100 + TauI GCSGC 1099 1096 . . 1096 1100 + BlsI GCNGC 1098 1097 . . 1096 1100 + BisI GCNGC 1097 1098 . . 1097 1100 + AciI CCGC 1097 1099 . . 1103 1108 + TatI WGTACW 1103 1107 . . 1103 1108 + Bsp1407I TGTACA 1103 1107 . . 1104 1107 + Csp6I GTAC 1104 1106 . . 1104 1107 + RsaI GTAC 1105 1105 . . 1133 1138 + TatI WGTACW 1133 1137 . . 1133 1138 + ScaI AGTACT 1135 1135 . . 1134 1137 + Csp6I GTAC 1134 1136 . . 1134 1137 + RsaI GTAC 1135 1135 . . 1137 1141 + DdeI CTNAG 1137 1140 . . 1154 1157 + SetI ASST 1157 1153 . . 1153 1156 - MnlI CCTC 1146 1145 . . 1162 1166 + HinfI GANTC 1162 1165 . . 1162 1166 - PleI GAGTC 1156 1157 . . 1162 1166 - MlyI GAGTC 1156 1156 . . 1169 1172 + TspEI AATT 1168 1172 . . 1176 1181 + PsiI TTATAA 1178 1178 . . 1180 1190 + FalI AAGNNNNNCTT 1171 1166 1203 1198 1180 1190 - FalI AAGNNNNNCTT 1203 1198 1171 1166 1200 1211 + BdaI TGANNNNNNTCA 1189 1187 1223 1221 1206 1210 + BsmAI GTCTC 1211 1215 . . 1200 1211 - BdaI TGANNNNNNTCA 1223 1221 1189 1187 1221 1225 + DdeI CTNAG 1221 1224 . . 1225 1229 + MboII GAAGA 1237 1236 . . 1225 1230 - Ksp632I* CTCTTC 1220 1223 . . 1237 1240 + SetI ASST 1240 1236 . . 1240 1243 + MseI TTAA 1240 1242 . . 1242 1245 + TspEI AATT 1241 1245 . . 1255 1259 + Hpy188I TCNGA 1257 1256 . . 1256 1261 + Eco57MI CTGRAG 1277 1275 . . 1256 1261 + Eco57I CTGAAG 1277 1275 . . 1258 1262 - Hin4II* CCTTC 1252 1251 . . 1293 1303 + EcoNI CCTNNNNNAGG 1297 1298 . . 1289 1293 - HphI GGTGA 1281 1280 . . 1293 1303 + BsiYI* CCNNNNNNNGG 1299 1296 . . 1300 1303 - MnlI CCTC 1293 1292 . . 1304 1307 + TspEI AATT 1303 1307 . . 1305 1310 + VspI ATTAAT 1306 1308 . . 1306 1309 + MseI TTAA 1306 1308 . . 1310 1313 + CviRI* TGCA 1311 1311 . . 1326 1329 + SetI ASST 1329 1325 . . 1324 1328 - Hin4II* CCTTC 1318 1317 . . 1328 1333 + Hpy166II GTNNAC 1330 1330 . . 1345 1348 + SetI ASST 1348 1344 . . 1346 1356 + BsiYI* CCNNNNNNNGG 1352 1349 . . 1351 1354 + TspEI AATT 1350 1354 . . 1358 1362 + TfiI GAWTC 1358 1361 . . 1358 1362 + HinfI GANTC 1358 1361 . . 1360 1364 - TspDTI ATGAA 1350 1348 . . 1367 1379 + PsrI GAACNNNNNNTAC 1359 1354 1391 1386 1375 1379 + MaeIII GTNAC 1374 1379 . . 1392 1397 - TaqII CACCCA 1382 1380 . . 1397 1402 + XhoII RGATCY 1397 1401 . . 1397 1401 + BinI* GGATC 1405 1406 . . 1398 1401 + DpnI GATC 1399 1399 . . 1398 1401 + BstKTI GATC 1400 1398 . . 1398 1401 + MboI GATC 1397 1401 . . 1412 1417 + PsiI TTATAA 1414 1414 . . 1416 1421 + SspI AATATT 1418 1418 . . 1424 1435 + BdaI TGANNNNNNTCA 1413 1411 1447 1445 1425 1434 + XmnI GAANNNNTTC 1429 1429 . . 1424 1435 - BdaI TGANNNNNNTCA 1447 1445 1413 1411 1432 1436 - TspDTI ATGAA 1422 1420 . . 1449 1452 + TspEI AATT 1448 1452 . . 1467 1471 + Tsp45I GTSAC 1466 1471 . . 1467 1471 + MaeIII GTNAC 1466 1471 . . 1465 1469 - HgaI GACGC 1454 1459 . . 1468 1472 - HphI GGTGA 1460 1459 . . 1474 1477 + MseI TTAA 1474 1476 . . 1477 1480 + CviJI RGCY 1478 1478 . . 1479 1493 + XcmI CCANNNNNNNNNTGG 1486 1485 . . 1480 1483 + FatI CATG 1479 1483 . . 1480 1483 + NlaIII CATG 1483 1479 . . 1480 1483 + CviAII CATG 1480 1482 . . 1482 1485 + CviRI* TGCA 1483 1483 . . 1492 1497 + NlaIV GGNNCC 1494 1494 . . 1502 1507 + ApoI RAATTY 1502 1506 . . 1503 1506 + TspEI AATT 1502 1506 . . 1513 1516 + TspEI AATT 1512 1516 . . 1539 1543 - TspGWI ACGGA 1529 1527 . . 1546 1549 + TspEI AATT 1545 1549 . . 1553 1558 + BspLU11I* ACATGT 1553 1557 . . 1553 1558 + NspI RCATGY 1557 1553 . . 1553 1558 + AflIII ACRYGT 1553 1557 . . 1554 1557 + FatI CATG 1553 1557 . . 1554 1557 + NlaIII CATG 1557 1553 . . 1554 1557 + CviAII CATG 1554 1556 . . 1557 1560 + Csp6I GTAC 1557 1559 . . 1557 1560 + RsaI GTAC 1558 1558 . . 1559 1562 + SetI ASST 1562 1558 . . 1560 1564 + Hin4II* CCTTC 1570 1569 . . 1570 1584 + XcmI CCANNNNNNNNNTGG 1577 1576 . . 1585 1588 + SetI ASST 1588 1584 . . 1585 1588 + CviJI RGCY 1586 1586 . . 1585 1588 + AluI AGCT 1586 1586 . . 1603 1608 + XhoII RGATCY 1603 1607 . . 1604 1607 + DpnI GATC 1605 1605 . . 1604 1607 + BstKTI GATC 1606 1604 . . 1604 1607 + MboI GATC 1603 1607 . . 1604 1608 - BinI* GGATC 1598 1599 . . 1609 1612 + Csp6I GTAC 1609 1611 . . 1609 1612 + RsaI GTAC 1610 1610 . . 1606 1610 - TspGWI ACGGA 1596 1594 . . 1614 1617 + TaiI ACGT 1617 1613 . . 1614 1617 + SetI ASST 1617 1613 . . 1614 1617 + MaeII ACGT 1614 1616 . . 1620 1623 + SetI ASST 1623 1619 . . 1627 1637 + FalI AAGNNNNNCTT 1618 1613 1650 1645 1630 1635 + EcoRI GAATTC 1630 1634 . . 1630 1635 + ApoI RAATTY 1630 1634 . . 1631 1634 + TspEI AATT 1630 1634 . . 1627 1637 - FalI AAGNNNNNCTT 1650 1645 1618 1613 1634 1638 - MboII GAAGA 1626 1625 . . 1641 1644 + SetI ASST 1644 1640 . . 1642 1647 + KpnI GGTACC 1646 1642 . . 1642 1647 + NlaIV GGNNCC 1644 1644 . . 1642 1647 + HgiCI* GGYRCC 1642 1646 . . 1642 1647 + Acc65I GGTACC 1642 1646 . . 1643 1646 + Csp6I GTAC 1643 1645 . . 1643 1646 + RsaI GTAC 1644 1644 . . 1645 1648 + SetI ASST 1648 1644 . . 1646 1649 + MnlI CCTC 1656 1655 . . 1646 1656 + BsiYI* CCNNNNNNNGG 1652 1649 . . 1651 1656 + StyI CCWWGG 1651 1655 . . 1651 1656 + SecI* CCNNGG 1651 1655 . . 1666 1669 + MseI TTAA 1666 1668 . . 1696 1699 - MnlI CCTC 1689 1688 . . 1701 1704 + TspEI AATT 1700 1704 . . 1724 1728 - BccI CCATC 1718 1719 . . 1729 1732 + TaiI ACGT 1732 1728 . . 1729 1732 + SetI ASST 1732 1728 . . 1729 1732 + MaeII ACGT 1729 1731 . . 1731 1736 + Hpy166II GTNNAC 1733 1733 . . 1740 1743 + TspEI AATT 1739 1743 . . 1779 1783 + HphI GGTGA 1791 1790 . . 1787 1790 + SetI ASST 1790 1786 . . 1787 1790 + CviJI RGCY 1788 1788 . . 1787 1790 + AluI AGCT 1788 1788 . . 1792 1796 - TspGWI ACGGA 1782 1780 . . 1808 1812 + BinI* GGATC 1816 1817 . . 1809 1812 + DpnI GATC 1810 1810 . . 1809 1812 + BstKTI GATC 1811 1809 . . 1809 1812 + MboI GATC 1808 1812 . . 1816 1821 + SpeI ACTAGT 1816 1820 . . 1817 1820 + MaeI CTAG 1817 1819 . . 1840 1843 + SetI ASST 1843 1839 . . 1849 1853 + HgaI GACGC 1858 1863 . . 1856 1859 + MseI TTAA 1856 1858 . . 1860 1863 + TspEI AATT 1859 1863 . . 1864 1867 - MnlI CCTC 1857 1856 . . 1885 1888 + MaeI CTAG 1885 1887 . . 1896 1901 + VspI ATTAAT 1897 1899 . . 1897 1900 + MseI TTAA 1897 1899 . . 1906 1910 + DdeI CTNAG 1906 1909 . . 1906 1910 + BspCNI CTCAG 1919 1917 . . 1906 1910 + BseMII CTCAG 1920 1918 . . 1908 1914 + TspRI CASTGNN 1914 1905 . . 1912 1922 + MwoI GCNNNNNNNGC 1918 1915 . . 1939 1945 + PfoI TCCNGGA 1939 1944 . . 1940 1944 + BssKI CCNGG 1939 1944 . . 1940 1944 + BseBI CCWGG 1941 1942 . . 1940 1944 + ScrFI CCNGG 1941 1942 . . 1940 1944 + EcoRII CCWGG 1939 1944 . . 1947 1952 + ApoI RAATTY 1947 1951 . . 1948 1951 + TspEI AATT 1947 1951 . . 1957 1962 + MluI ACGCGT 1957 1961 . . 1957 1962 + AflIII ACRYGT 1957 1961 . . 1958 1961 + FnuDII* CGCG 1959 1959 . . 1959 1964 + Cac8I GCNNGC 1961 1961 . . 1963 1973 + MwoI GCNNNNNNNGC 1969 1966 . . 1979 1984 + ApoI RAATTY 1979 1983 . . 1980 1983 + TspEI AATT 1979 1983 . . 1985 1990 + StyI CCWWGG 1985 1989 . . 1985 1990 + SecI* CCNNGG 1985 1989 . . 1992 1995 + CviJI RGCY 1993 1993 . . 1993 1997 + BlsI GCNGC 1995 1994 . . 1993 1997 + BisI GCNGC 1994 1995 . . 1993 1997 + TseI GCWGC 1993 1996 . . 1996 2006 + MwoI GCNNNNNNNGC 2002 1999 . . 1996 2006 + BglI GCCNNNNNGGC 2002 1999 . . 1993 1997 - BbvI GCAGC 1980 1984 . . 1998 2001 + FatI CATG 1997 2001 . . 1998 2001 + NlaIII CATG 2001 1997 . . 1998 2001 + CviAII CATG 1998 2000 . . 2008 2012 + MaeIII GTNAC 2007 2012 . . 2005 2008 - AciI CCGC 2005 2007 . . 2036 2039 + SetI ASST 2039 2035 . . 2036 2039 + CviJI RGCY 2037 2037 . . 2036 2039 + AluI AGCT 2037 2037 . . 2038 2042 + DdeI CTNAG 2038 2041 . . 2048 2054 + BstEII GGTNACC 2048 2053 . . 2049 2053 + MaeIII GTNAC 2048 2053 . . 2059 2063 + PleI GAGTC 2067 2068 . . 2059 2063 + MlyI GAGTC 2068 2068 . . 2059 2063 + HinfI GANTC 2059 2062 . . 2061 2065 + Tsp45I GTSAC 2060 2065 . . 2061 2065 + MaeIII GTNAC 2060 2065 . . 2066 2069 + SetI ASST 2069 2065 . . 2074 2078 + FokI GGATG 2087 2091 . . 2074 2078 + BseGI GGATG 2080 2078 . . 2075 2079 - BccI CCATC 2069 2070 . . 2082 2085 + TspEI AATT 2081 2085 . . 2112 2115 + TspEI AATT 2111 2115 . . 2129 2132 + CviJI RGCY 2130 2130 . . 2153 2163 + Hin4I GAYNNNNNVTC 2144 2139 2176 2171 2159 2164 + BclI TGATCA 2159 2163 . . 2160 2163 + DpnI GATC 2161 2161 . . 2160 2163 + BstKTI GATC 2162 2160 . . 2160 2163 + MboI GATC 2159 2163 . . 2153 2163 - Hin4I GAYNNNNNVTC 2176 2171 2144 2139 2170 2173 + MseI TTAA 2170 2172 . . 2173 2176 + SetI ASST 2176 2172 . . 2174 2178 + Hin4II* CCTTC 2184 2183 . . 2182 2186 + BccI CCATC 2190 2191 . . 2183 2187 - FokI GGATG 2169 2173 . . 2183 2187 - BseGI GGATG 2182 2180 . . 2209 2214 + AsuII TTCGAA 2210 2212 . . 2210 2213 + TaqI TCGA 2210 2212 . . 2212 2216 + TfiI GAWTC 2212 2215 . . 2212 2216 + HinfI GANTC 2212 2215 . . 2232 2237 + Hpy166II GTNNAC 2234 2234 . . 2236 2241 + SpeI ACTAGT 2236 2240 . . 2237 2240 + MaeI CTAG 2237 2239 . . 2240 2244 + MaeIII GTNAC 2239 2244 . . 2244 2247 + FatI CATG 2243 2247 . . 2244 2247 + NlaIII CATG 2247 2243 . . 2244 2247 + CviAII CATG 2244 2246 . . 2248 2251 + Csp6I GTAC 2248 2250 . . 2248 2251 + RsaI GTAC 2249 2249 . . 2262 2265 + TspEI AATT 2261 2265 . . 2284 2288 + TspDTI ATGAA 2299 2297 . . 2286 2290 + MboII GAAGA 2298 2297 . . 2286 2291 + BbvII* GAAGAC 2293 2297 . . 2300 2304 + TspDTI ATGAA 2315 2313 . . 2305 2308 + TspEI AATT 2304 2308 . . 2310 2314 + MaeIII GTNAC 2309 2314 . . 2308 2313 - TsoI TARCCA 2298 2296 . . 2320 2324 + DdeI CTNAG 2320 2323 . . 2359 2364 - BsmI GAATGC 2359 2357 . . 2371 2375 + BsrI ACTGG 2376 2374 . . 2375 2379 + MaeIII GTNAC 2374 2379 . . 2378 2381 + TaiI ACGT 2381 2377 . . 2378 2381 + SetI ASST 2381 2377 . . 2378 2381 + MaeII ACGT 2378 2380 . . 2379 2383 + Hpy99I CGWCG 2383 2378 . . 2375 2387 - BarI GAAGNNNNNNTAC 2399 2394 2367 2362 2391 2396 + Ksp632I* CTCTTC 2397 2400 . . 2392 2396 - MboII GAAGA 2384 2383 . . 2394 2398 - TspDTI ATGAA 2384 2382 . . 2400 2404 - TspDTI ATGAA 2390 2388 . . 2407 2411 + PleI GAGTC 2415 2416 . . 2407 2411 + MlyI GAGTC 2416 2416 . . 2407 2411 + HinfI GANTC 2407 2410 . . 2430 2433 + TspEI AATT 2429 2433 . . 2437 2440 + DpnI GATC 2438 2438 . . 2437 2440 + BstKTI GATC 2439 2437 . . 2437 2440 + MboI GATC 2436 2440 . . 2437 2441 - BinI* GGATC 2431 2432 . . 2444 2449 + Hpy178III* TCNNGA 2445 2447 . . 2448 2452 - BccI CCATC 2442 2443 . . 2453 2456 + Hin6I GCGC 2453 2455 . . 2453 2456 + GlaI GCGC 2454 2454 . . 2453 2456 + HhaI GCGC 2455 2453 . . 2471 2474 + TspEI AATT 2470 2474 . . 2478 2481 + TspEI AATT 2477 2481 . . 2489 2492 + CviJI RGCY 2490 2490 . . 2496 2501 + Hpy166II GTNNAC 2498 2498 . . 2500 2505 + NspI RCATGY 2504 2500 . . 2501 2504 + FatI CATG 2500 2504 . . 2501 2504 + NlaIII CATG 2504 2500 . . 2501 2504 + CviAII CATG 2501 2503 . . 2505 2510 + StyI CCWWGG 2505 2509 . . 2505 2510 + SecI* CCNNGG 2505 2509 . . 2505 2510 + AvrII CCTAGG 2505 2509 . . 2506 2509 + MaeI CTAG 2506 2508 . . 2508 2511 + SetI ASST 2511 2507 . . 2510 2515 + Hpy166II GTNNAC 2512 2512 . . 2510 2515 + BssNAI GTATAC 2512 2512 . . 2510 2515 + AccI GTMKAC 2511 2513 . . 2517 2522 + ApoI RAATTY 2517 2521 . . 2518 2521 + TspEI AATT 2517 2521 . . 2520 2525 + AhaIII* TTTAAA 2522 2522 . . 2521 2524 + MseI TTAA 2521 2523 . . 2528 2531 + MseI TTAA 2528 2530 . . 2556 2561 + VspI ATTAAT 2557 2559 . . 2557 2560 + MseI TTAA 2557 2559 . . 2559 2562 + TspEI AATT 2558 2562 . . 2568 2571 + FatI CATG 2567 2571 . . 2568 2571 + NlaIII CATG 2571 2567 . . 2568 2571 + CviAII CATG 2568 2570 . . 2575 2585 + Hin4I GAYNNNNNVTC 2566 2561 2598 2593 2575 2585 - Hin4I GAYNNNNNVTC 2598 2593 2566 2561 2589 2598 + XmnI GAANNNNTTC 2593 2593 . . 2589 2594 + ApoI RAATTY 2589 2593 . . 2590 2593 + TspEI AATT 2589 2593 . . 2600 2603 + SetI ASST 2603 2599 . . 2605 2608 + MseI TTAA 2605 2607 . . 2611 2615 + TspGWI ACGGA 2626 2624 . . 2614 2617 - MnlI CCTC 2607 2606 . . 2613 2623 - BsaXI ACNNNNNCTCC 2635 2632 2605 2602 2613 2624 - TstI CACNNNNNNTCC 2637 2632 2605 2600 2625 2630 - MmeI TCCRAC 2606 2604 . . 2639 2644 + TsoI TARCCA 2655 2653 . . 2647 2652 + MmeI TCCRAC 2672 2670 . . 2648 2662 + XcmI CCANNNNNNNNNTGG 2655 2654 . . 2664 2667 + DpnI GATC 2665 2665 . . 2664 2667 + BstKTI GATC 2666 2664 . . 2664 2667 + MboI GATC 2663 2667 . . 2664 2668 - BinI* GGATC 2658 2659 . . 2682 2685 + FatI CATG 2681 2685 . . 2682 2685 + NlaIII CATG 2685 2681 . . 2682 2685 + CviAII CATG 2682 2684 . . 2680 2684 - TspDTI ATGAA 2670 2668 . . 2697 2702 + TatI WGTACW 2697 2701 . . 2697 2702 + ScaI AGTACT 2699 2699 . . 2698 2701 + Csp6I GTAC 2698 2700 . . 2698 2701 + RsaI GTAC 2699 2699 . . 2702 2707 - TaqII GACCGA 2692 2690 . . 2707 2710 + FatI CATG 2706 2710 . . 2707 2710 + NlaIII CATG 2710 2706 . . 2707 2710 + CviAII CATG 2707 2709 . . #--------------------------------------- # # # Acc65I: # [GGTACC] # 1118 1642 # # AccI: # [GTMKAC] # 249 2511 # # AciI: # [CCGC] # 13 94 98 208 684 755 # 908 # # AflIII: # [ACRYGT] # 404 803 1553 # # AhaIII*: # [TTTAAA] # 238 2522 # # AjuI: # [GAANNNNNNNTTGG] # 32 595 2133 # # AluI: # [AGCT] # 138 202 249 405 494 549 # 723 # # ApoI: # [RAATTY] # 32 72 128 171 229 317 # 464 538 809 # # AsuI*: # [GGNCC] # 866 1894 # # AsuII: # [TTCGAA] # 550 2210 # # AvaII: # [GGWCC] # 866 1894 # # AvrII: # [CCTAGG] # 255 2505 # # BarI: # [GAAGNNNNNNTAC] # 32 361 2367 # # BbvI: # [GCAGC] # 780 1980 # # BbvII*: # [GAAGAC] # 467 2293 # # BccI: # [CCATC] # 87 121 163 252 300 318 # 351 516 652 # # BceAI: # [ACGGC] # 346 2414 # # BcgI: # [CGANNNNNNTGC] # 34 535 2191 # # BciVI: # [GTATCC] # 225 711 1824 # # BclI: # [TGATCA] # 225 510 601 1424 # # BdaI: # [TGANNNNNNTCA] # 0 0 0 0 34 34 # 190 1189 1313 # # BetI*: # [WCCGGW] # 217 704 1839 # # BglI: # [GCCNNNNNGGC] # 758 2002 # # BglII: # [AGATCT] # 250 2510 # # BinI*: # [GGATC] # 102 193 218 227 256 565 # 584 615 # # BisI: # [GCNGC] # 766 897 1097 # # BlsI: # [GCNGC] # 765 897 1098 # # BmgT120I: # [GGNCC] # 867 1893 # # BsaAI: # [YACGTR] # 178 2582 # # BsaXI: # [ACNNNNNCTCC] # 30 125 2605 # # BseBI: # [CCWGG] # 317 819 1624 # # BseGI: # [GGATG] # 49 102 207 291 302 578 # 1231 # # BseMII: # [CTCAG] # 840 1920 # # BseYI: # [CCCAGC] # 337 2423 # # BsgI: # [GTGCAG] # 200 2560 # # BsiYI*: # [CCNNNNNNNGG] # 53 217 300 1082 1108 # # BsmAI: # [GTCTC] # 1211 1549 # # BsmI: # [GAATGC] # 302 401 2057 # # Bsp1407I: # [TGTACA] # 1103 1657 # # BspCNI: # [CTCAG] # 841 1919 # # BspLU11I*: # [ACATGT] # 1207 1553 # # BspMII*: # [TCCGGA] # 217 2543 # # BsrBI: # [CCGCTC] # 96 2664 # # BsrI: # [ACTGG] # 211 384 693 1472 # # BssKI: # [CCNGG] # 315 821 1624 # # BssNAI: # [GTATAC] # 248 2512 # # BstAPI: # [GCANNNNNTGC] # 994 1766 # # BstEII: # [GGTNACC] # 712 2048 # # BstKTI: # [GATC] # 22 66 75 94 159 205 # 206 227 253 260 277 351 # 565 # # BstXI: # [CCANNNNNNTGG] # 549 2211 # # Cac8I: # [GCNNGC] # 108 799 867 986 # # ClaI: # [ATCGAT] # 1070 1690 # # Csp6I: # [GTAC] # 30 34 52 62 125 197 # 423 450 605 782 # # CviAII: # [CATG] # 24 25 53 67 74 103 # 114 219 246 257 444 492 # 642 # # CviJI: # [RGCY] # 31 44 82 93 108 138 # 202 205 270 360 386 405 # 436 # # CviRI*: # [TGCA] # 39 142 172 485 645 1277 # # DdeI: # [CTNAG] # 84 132 282 440 685 1137 # # DpnI: # [GATC] # 22 66 75 95 159 205 # 206 227 252 260 277 351 # 565 # # DraII: # [RGGNCCY] # 866 1894 # # DraIII: # [CACNNNGTG] # 841 1919 # # Eco57I: # [CTGAAG] # 1277 1483 # # Eco57MI: # [CTGRAG] # 1277 1483 # # EcoNI: # [CCTNNNNNAGG] # 1297 1463 # # EcoP15I: # [CAGCAG] # 836 1924 # # EcoRI: # [GAATTC] # 1130 1630 # # EcoRII: # [CCWGG] # 315 821 1624 # # EcoRV: # [GATATC] # 78 2682 # # FalI: # [AAGNNNNNCTT] # 0 0 0 0 32 32 # 415 1110 1171 # # FatI: # [CATG] # 24 25 54 67 74 102 # 114 219 246 257 444 492 # 642 # # FauI: # [CCCGC] # 882 1878 # # FnuDII*: # [CGCG] # 801 1959 # # FokI: # [GGATG] # 49 82 194 271 322 591 # 1251 # # GlaI: # [GCGC] # 306 474 1980 # # GsaI: # [CCCAGC] # 341 2419 # # HaeII: # [RGCGCY] # 476 2284 # # HgaI: # [GACGC] # 30 404 902 1424 # # HgiCI*: # [GGYRCC] # 237 1118 1405 # # HgiJII*: # [GRGCYC] # 115 2645 # # HhaI: # [GCGC] # 305 475 1980 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 0 0 # 32 32 32 32 102 162 # 361 390 1617 # # Hin4II*: # [CCTTC] # 66 252 576 614 1252 # # Hin6I: # [GCGC] # 307 473 1980 # # HindII: # [GTYRAC] # 270 495 1995 # # HindIII: # [AAGCTT] # 1090 1670 # # HinfI: # [GANTC] # 153 195 196 307 353 701 # 855 # # HpaII: # [CCGG] # 218 245 459 1838 # # HphI: # [GGTGA] # 83 95 179 207 221 256 # 331 419 969 # # Hpy166II: # [GTNNAC] # 14 53 175 248 261 264 # 267 270 304 403 501 # # Hpy178III*: # [TCNNGA] # 218 315 2227 # # Hpy188I: # [TCNGA] # 327 450 480 1503 # # Hpy99I: # [CGWCG] # 24 377 2359 # # KpnI: # [GGTACC] # 1114 1646 # # Ksp632I*: # [CTCTTC] # 363 1177 1220 # # MaeI: # [CTAG] # 68 254 269 352 661 1156 # # MaeII: # [ACGT] # 115 177 382 649 1437 # # MaeIII: # [GTNAC] # 12 32 41 65 70 92 # 162 179 201 211 290 386 # 478 541 # # MboI: # [GATC] # 22 66 75 97 159 205 # 206 227 250 260 277 351 # 565 # # MboII: # [GAAGA] # 86 169 361 376 389 672 # 707 # # MluI: # [ACGCGT] # 803 1957 # # MlyI: # [GAGTC] # 344 348 912 1156 # # MmeI: # [TCCRAC] # 66 88 154 201 598 1653 # # MnlI: # [CCTC] # 33 146 147 153 168 265 # 363 365 370 750 # # MseI: # [TTAA] # 7 29 38 41 48 66 # 155 168 190 192 237 273 # 351 965 # # MslI: # [CAYNNNNRTG] # 502 2258 # # MwoI: # [GCNNNNNNNGC] # 33 51 108 334 552 758 # 924 # # NlaIII: # [CATG] # 24 25 50 67 74 106 # 114 219 246 257 444 492 # 642 # # NlaIV: # [GGNNCC] # 150 239 626 629 1116 # # NmeAIII: # [GCCGAG] # 729 2031 # # NspI: # [RCATGY] # 256 947 1557 # # PfoI: # [TCCNGGA] # 821 1939 # # PleI: # [GAGTC] # 345 348 911 1156 # # PpuMI: # [RGGWCCY] # 866 1894 # # PsiI: # [TTATAA] # 236 1178 1346 # # PsrI: # [GAACNNNNNNTAC] # 32 1359 1369 # # RsaI: # [GTAC] # 30 34 52 61 126 197 # 423 450 605 782 # # ScaI: # [AGTACT] # 61 126 1009 1564 # # ScrFI: # [CCNGG] # 317 819 1624 # # SduI: # [GDGCHC] # 115 2645 # # SecI*: # [CCNNGG] # 255 334 520 1651 # # SetI: # [ASST] # 4 6 12 19 21 26 # 29 30 34 53 58 62 # 63 63 72 83 84 89 # 92 92 98 107 115 118 # 130 138 157 196 205 214 # 290 # # SfaNI: # [GCATC] # 543 2217 # # SpeI: # [ACTAGT] # 420 524 1816 # # SspI: # [AATATT] # 1342 1418 # # StyI: # [CCWWGG] # 255 334 520 1651 # # TaiI: # [ACGT] # 115 180 379 649 1437 # # TaqI: # [TCGA] # 24 112 123 398 413 550 # 1140 # # TaqII: # [CACCCA] # 68 1310 1382 # # TatI: # [WGTACW] # 30 63 124 197 782 1564 # # TauI: # [GCSGC] # 1099 1661 # # TfiI: # [GAWTC] # 307 548 854 1051 # # TseI: # [GCWGC] # 767 1993 # # TsoI: # [TARCCA] # 105 357 2298 # # Tsp45I: # [GTSAC] # 162 201 243 382 478 594 # 700 # # Tsp4CI*: # [ACNGT] # 210 280 357 1913 # # TspDTI: # [ATGAA] # 6 16 16 37 38 69 # 72 90 145 186 213 280 # 715 877 # # TspEI: # [AATT] # 7 10 11 30 31 32 # 32 33 39 40 41 41 # 43 47 54 62 70 73 # 85 88 93 98 102 120 # 125 130 150 164 171 187 # 229 322 # # TspGWI: # [ACGGA] # 67 134 186 844 1529 # # TspRI: # [CASTGNN] # 605 846 1309 # # TstI: # [CACNNNNNNTCC] # 32 123 2605 # # Tth111I: # [GACNNNGTC] # 42 718 2000 # # VspI: # [ATTAAT] # 203 591 660 1306 # # XcmI: # [CCANNNNNNNNNTGG] # 91 105 1078 1486 # # XhoII: # [RGATCY] # 206 250 1147 1157 # # XmnI: # [GAANNNNTTC] # 167 1164 1429 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 2760 # Reported_sequences: 1 # Reported_hitcount: 529 #---------------------------------------