Restriction Fragments for UTP11/YKL099C



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The currently selected gene/sequence is :

UTP11/YKL099C on chromosome XI from coordinates 256472 to 255720.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.19586.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 3 6 + CviJI RGCY 4 4 . . 12 17 + TatI WGTACW 12 16 . . 12 17 + Bsp1407I TGTACA 12 16 . . 13 16 + Csp6I GTAC 13 15 . . 13 16 + RsaI GTAC 14 14 . . 16 19 + FatI CATG 15 19 . . 16 19 + NlaIII CATG 19 15 . . 16 19 + CviAII CATG 16 18 . . 24 28 + Hpy188I TCNGA 26 25 . . 13 25 - PsrI GAACNNNNNNTAC 37 32 5 0 33 37 + BlsI GCNGC 35 34 . . 33 37 + BisI GCNGC 34 35 . . 33 37 + BbvI GCAGC 45 49 . . 33 37 + TseI GCWGC 33 36 . . 44 49 + AclI AACGTT 45 47 . . 45 48 + TaiI ACGT 48 44 . . 45 48 + SetI ASST 48 44 . . 45 48 + MaeII ACGT 45 47 . . 50 54 + DdeI CTNAG 50 53 . . 50 54 + BspCNI CTCAG 63 61 . . 50 54 + BseMII CTCAG 64 62 . . 53 56 + SetI ASST 56 52 . . 53 56 + CviJI RGCY 54 54 . . 53 56 + AluI AGCT 54 54 . . 59 62 + MaeI CTAG 59 61 . . 61 64 + CviJI RGCY 62 62 . . 62 66 - BceAI ACGGC 47 49 . . 67 72 + Hpy178III* TCNNGA 68 70 . . 68 78 + BsiYI* CCNNNNNNNGG 74 71 . . 108 111 + TaiI ACGT 111 107 . . 108 111 + SetI ASST 111 107 . . 108 111 + MaeII ACGT 108 110 . . 110 115 + SduI GDGCHC 114 110 . . 110 115 + HgiAI* GWGCWC 114 110 . . 113 118 + SmlI CTYRAG 113 117 . . 114 119 + Hpy178III* TCNNGA 115 117 . . 113 118 - Bce83I* CTTGAG 98 96 . . 135 140 + Hpy166II GTNNAC 137 137 . . 143 154 + BdaI TGANNNNNNTCA 132 130 166 164 150 153 + MnlI CCTC 160 159 . . 151 155 + DdeI CTNAG 151 154 . . 151 155 + BspCNI CTCAG 164 162 . . 151 155 + BseMII CTCAG 165 163 . . 152 156 + Hpy188I TCNGA 154 153 . . 143 154 - BdaI TGANNNNNNTCA 166 164 132 130 177 182 + Hpy178III* TCNNGA 178 180 . . 182 186 + TspDTI ATGAA 197 195 . . 193 196 + FatI CATG 192 196 . . 193 196 + NlaIII CATG 196 192 . . 193 196 + CviAII CATG 193 195 . . 199 204 + EcoT22I ATGCAT 203 199 . . 200 203 + CviRI* TGCA 201 201 . . 201 206 - BsmI GAATGC 201 199 . . 210 214 + MboII GAAGA 222 221 . . 210 215 + BbvII* GAAGAC 217 221 . . 219 222 + CviRI* TGCA 220 220 . . 217 221 - SfaNI GCATC 207 211 . . 227 237 + Hin4I GAYNNNNNVTC 218 213 250 245 233 238 + BclI TGATCA 233 237 . . 229 234 - EcoP15I CAGCAG 201 203 . . 234 237 + DpnI GATC 235 235 . . 234 237 + BstKTI GATC 236 234 . . 234 237 + MboI GATC 233 237 . . 227 237 - Hin4I GAYNNNNNVTC 250 245 218 213 238 241 + SetI ASST 241 237 . . 238 241 + CviJI RGCY 239 239 . . 238 241 + AluI AGCT 239 239 . . 242 247 + BtrI CACGTC 244 244 . . 243 246 + TaiI ACGT 246 242 . . 243 246 + SetI ASST 246 242 . . 243 246 + MaeII ACGT 243 245 . . 245 249 + Tsp45I GTSAC 244 249 . . 245 249 + MaeIII GTNAC 244 249 . . 248 252 + Tsp4CI* ACNGT 250 249 . . 250 254 + HphI GGTGA 262 261 . . 251 255 + Tsp45I GTSAC 250 255 . . 251 255 + MaeIII GTNAC 250 255 . . 256 260 + MboII GAAGA 268 267 . . 259 263 + MboII GAAGA 271 270 . . 259 264 + BbvII* GAAGAC 266 270 . . 265 269 + TfiI GAWTC 265 268 . . 265 269 + HinfI GANTC 265 268 . . 275 280 + DsaI* CCRYGG 275 279 . . 275 280 + StyI CCWWGG 275 279 . . 275 280 + NcoI CCATGG 275 279 . . 275 280 + SecI* CCNNGG 275 279 . . 276 279 + FatI CATG 275 279 . . 276 279 + NlaIII CATG 279 275 . . 276 279 + CviAII CATG 276 278 . . 279 283 + BinI* GGATC 287 288 . . 280 283 + DpnI GATC 281 281 . . 280 283 + BstKTI GATC 282 280 . . 280 283 + MboI GATC 279 283 . . 287 290 + MseI TTAA 287 289 . . 290 293 + TspEI AATT 289 293 . . 313 316 + TspEI AATT 312 316 . . 321 324 - MnlI CCTC 314 313 . . 324 328 + HgaI GACGC 333 338 . . 328 332 + DdeI CTNAG 328 331 . . 328 332 - BspCNI CTCAG 320 318 . . 328 332 - BseMII CTCAG 319 317 . . 330 333 - MnlI CCTC 323 322 . . 333 343 + MwoI GCNNNNNNNGC 339 336 . . 327 333 - Bpu10I CCTNAGC 328 331 . . 327 333 - BbvCI CCTCAGC 328 331 . . 341 344 + SetI ASST 344 340 . . 341 344 + CviJI RGCY 342 342 . . 341 344 + AluI AGCT 342 342 . . 342 352 + MwoI GCNNNNNNNGC 348 345 . . 350 353 + SetI ASST 353 349 . . 350 353 + CviJI RGCY 351 351 . . 350 353 + AluI AGCT 351 351 . . 352 357 + Eco57MI CTGRAG 373 371 . . 352 357 + GsuI CTGGAG 373 371 . . 369 373 + Tsp4CI* ACNGT 371 370 . . 381 392 + CspCI CAANNNNNGTGG 369 367 404 402 386 391 + BtsI GCAGTG 393 391 . . 387 393 + TspRI CASTGNN 393 384 . . 393 397 + TfiI GAWTC 393 396 . . 393 397 + HinfI GANTC 393 396 . . 402 407 + SspI AATATT 404 404 . . 408 412 + Hpy99I CGWCG 412 407 . . 410 413 + TaqI TCGA 410 412 . . 412 416 + TfiI GAWTC 412 415 . . 412 416 + HinfI GANTC 412 415 . . 415 420 + Hpy178III* TCNNGA 416 418 . . 416 421 + BmeT110I CYCGRG 417 419 . . 416 421 + AvaI CYCGRG 416 420 . . 423 427 + MboII GAAGA 435 434 . . 427 431 + TspDTI ATGAA 442 440 . . 428 439 + BdaI TGANNNNNNTCA 417 415 451 449 429 438 + XmnI GAANNNNTTC 433 433 . . 433 438 + EcoRI GAATTC 433 437 . . 433 438 + ApoI RAATTY 433 437 . . 434 437 + TspEI AATT 433 437 . . 428 439 - BdaI TGANNNNNNTCA 451 449 417 415 437 441 - HphI GGTGA 429 428 . . 447 456 + XmnI GAANNNNTTC 451 451 . . 461 475 + XcmI CCANNNNNNNNNTGG 468 467 . . 463 466 + SetI ASST 466 462 . . 464 467 + MnlI CCTC 474 473 . . 466 470 + Hpy188I TCNGA 468 467 . . 474 478 + HphI GGTGA 486 485 . . 471 475 - BccI CCATC 465 466 . . 481 486 + XhoII RGATCY 481 485 . . 481 486 + BglII AGATCT 481 485 . . 482 485 + DpnI GATC 483 483 . . 482 485 + BstKTI GATC 484 482 . . 482 485 + MboI GATC 481 485 . . 484 488 + Hpy188I TCNGA 486 485 . . 496 499 + MseI TTAA 496 498 . . 500 504 + DdeI CTNAG 500 503 . . 504 508 + BmgT120I GGNCC 505 506 . . 504 508 + AvaII GGWCC 504 507 . . 504 508 + AsuI* GGNCC 504 507 . . 509 512 + TspEI AATT 508 512 . . 513 516 + CviJI RGCY 514 514 . . 543 547 - TspDTI ATGAA 533 531 . . 562 566 + TfiI GAWTC 562 565 . . 562 566 + HinfI GANTC 562 565 . . 581 584 + TspEI AATT 580 584 . . 584 595 + BdaI TGANNNNNNTCA 573 571 607 605 585 594 + XmnI GAANNNNTTC 589 589 . . 584 595 - BdaI TGANNNNNNTCA 607 605 573 571 597 607 + MwoI GCNNNNNNNGC 603 600 . . 604 614 + FalI AAGNNNNNCTT 595 590 627 622 606 610 + BlsI GCNGC 608 607 . . 606 610 + BisI GCNGC 607 608 . . 606 610 + BbvI GCAGC 618 622 . . 606 610 + TseI GCWGC 606 609 . . 604 614 - FalI AAGNNNNNCTT 627 622 595 590 618 628 + BplI GAGNNNNNCTC 609 604 641 636 618 621 - MnlI CCTC 611 610 . . 628 633 + MfeI CAATTG 628 632 . . 618 628 - BplI GAGNNNNNCTC 641 636 609 604 629 632 + TspEI AATT 628 632 . . 638 641 + SetI ASST 641 637 . . 639 643 + BmgT120I GGNCC 640 641 . . 639 643 + AvaII GGWCC 639 642 . . 639 643 + AsuI* GGNCC 639 642 . . 641 646 + MmeI TCCRAC 666 664 . . 654 658 + FokI GGATG 667 671 . . 654 658 + BseGI GGATG 660 658 . . 655 659 - SfaNI GCATC 645 649 . . 668 671 + TspEI AATT 667 671 . . 675 679 + MboII GAAGA 687 686 . . 678 682 - Hin4II* CCTTC 672 671 . . 682 687 + NlaIV GGNNCC 684 684 . . 700 705 + Hpy166II GTNNAC 702 702 . . 700 705 + AccI GTMKAC 701 703 . . 703 707 + HinfI GANTC 703 706 . . 705 710 + Ksp632I* CTCTTC 711 714 . . 703 707 - PleI GAGTC 697 698 . . 703 707 - MlyI GAGTC 697 697 . . 709 714 + Hpy178III* TCNNGA 710 712 . . 706 710 - MboII GAAGA 698 697 . . 707 712 - Eco57MI CTGRAG 692 690 . . 707 712 - Eco57I CTGAAG 692 690 . . 713 722 + XmnI GAANNNNTTC 717 717 . . 716 721 + ApoI RAATTY 716 720 . . 717 720 + TspEI AATT 716 720 . . 722 731 + MslI CAYNNNNRTG 726 726 . . 723 730 + SwaI ATTTAAAT 726 726 . . 720 724 - TspDTI ATGAA 710 708 . . 724 729 + AhaIII* TTTAAA 726 726 . . 725 728 + MseI TTAA 725 727 . . 732 736 + MboII GAAGA 744 743 . . 746 751 + AclI AACGTT 747 749 . . 747 750 + TaiI ACGT 750 746 . . 747 750 + SetI ASST 750 746 . . 747 750 + MaeII ACGT 747 749 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 52 701 # # AclI: # [AACGTT] # 6 45 702 # # AhaIII*: # [TTTAAA] # 27 726 # # AluI: # [AGCT] # 9 54 103 185 402 # # ApoI: # [RAATTY] # 37 283 433 # # AsuI*: # [GGNCC] # 114 135 504 # # AvaI: # [CYCGRG] # 337 416 # # AvaII: # [GGWCC] # 114 135 504 # # BbvCI: # [CCTCAGC] # 328 425 # # BbvI: # [GCAGC] # 45 135 573 # # BbvII*: # [GAAGAC] # 49 217 487 # # BccI: # [CCATC] # 288 465 # # Bce83I*: # [CTTGAG] # 98 655 # # BceAI: # [ACGGC] # 47 706 # # BclI: # [TGATCA] # 233 520 # # BdaI: # [TGANNNNNNTCA] # 0 0 0 0 0 0 # 34 34 34 122 132 146 # 251 # # BglII: # [AGATCT] # 272 481 # # BinI*: # [GGATC] # 287 466 # # BisI: # [GCNGC] # 34 146 573 # # BlsI: # [GCNGC] # 35 145 573 # # BmeT110I: # [CYCGRG] # 336 417 # # BmgT120I: # [GGNCC] # 113 135 505 # # BplI: # [GAGNNNNNCTC] # 0 0 32 112 609 # # Bpu10I: # [CCTNAGC] # 328 425 # # BseGI: # [GGATG] # 93 660 # # BseMII: # [CTCAG] # 64 101 154 434 # # BsiYI*: # [CCNNNNNNNGG] # 74 679 # # BsmI: # [GAATGC] # 201 552 # # Bsp1407I: # [TGTACA] # 12 741 # # BspCNI: # [CTCAG] # 63 101 156 433 # # BstKTI: # [GATC] # 46 202 236 269 # # BtrI: # [CACGTC] # 244 509 # # BtsI: # [GCAGTG] # 360 393 # # Csp6I: # [GTAC] # 13 740 # # CspCI: # [CAANNNNNGTGG] # 35 349 369 # # CviAII: # [CATG] # 16 83 177 477 # # CviJI: # [RGCY] # 4 8 9 50 103 163 # 177 239 # # CviRI*: # [TGCA] # 19 201 533 # # DdeI: # [CTNAG] # 50 101 172 177 253 # # DpnI: # [GATC] # 46 202 235 270 # # DsaI*: # [CCRYGG] # 275 478 # # Eco57I: # [CTGAAG] # 61 692 # # Eco57MI: # [CTGRAG] # 61 319 373 # # EcoP15I: # [CAGCAG] # 201 552 # # EcoRI: # [GAATTC] # 320 433 # # EcoT22I: # [ATGCAT] # 203 550 # # FalI: # [AAGNNNNNCTT] # 0 0 32 126 595 # # FatI: # [CATG] # 15 83 177 478 # # FokI: # [GGATG] # 86 667 # # GsuI: # [CTGGAG] # 373 380 # # HgaI: # [GACGC] # 333 420 # # HgiAI*: # [GWGCWC] # 114 639 # # Hin4I: # [GAYNNNNNVTC] # 0 0 32 218 503 # # Hin4II*: # [CCTTC] # 81 672 # # HinfI: # [GANTC] # 19 50 128 141 150 265 # # HphI: # [GGTGA] # 57 167 262 267 # # Hpy166II: # [GTNNAC] # 51 137 565 # # Hpy178III*: # [TCNNGA] # 43 47 63 68 238 294 # # Hpy188I: # [TCNGA] # 18 26 128 267 314 # # Hpy99I: # [CGWCG] # 341 412 # # Ksp632I*: # [CTCTTC] # 42 711 # # MaeI: # [CTAG] # 59 694 # # MaeII: # [ACGT] # 6 45 63 135 504 # # MaeIII: # [GTNAC] # 6 244 503 # # MboI: # [GATC] # 46 202 233 272 # # MboII: # [GAAGA] # 3 9 11 46 46 164 # 222 252 # # MfeI: # [CAATTG] # 125 628 # # MlyI: # [GAGTC] # 56 697 # # MmeI: # [TCCRAC] # 87 666 # # MnlI: # [CCTC] # 9 137 142 151 154 160 # # MseI: # [TTAA] # 28 209 229 287 # # MslI: # [CAYNNNNRTG] # 27 726 # # MwoI: # [GCNNNNNNNGC] # 9 150 255 339 # # NcoI: # [CCATGG] # 275 478 # # NlaIII: # [CATG] # 19 83 177 474 # # NlaIV: # [GGNNCC] # 69 684 # # PleI: # [GAGTC] # 56 697 # # PsrI: # [GAACNNNNNNTAC] # 5 32 716 # # RsaI: # [GTAC] # 14 739 # # SduI: # [GDGCHC] # 114 639 # # SecI*: # [CCNNGG] # 275 478 # # SetI: # [ASST] # 3 5 8 9 48 55 # 98 109 113 130 175 # # SfaNI: # [GCATC] # 108 207 438 # # SmlI: # [CTYRAG] # 113 640 # # SspI: # [AATATT] # 349 404 # # StyI: # [CCWWGG] # 275 478 # # SwaI: # [ATTTAAAT] # 27 726 # # TaiI: # [ACGT] # 3 48 63 135 504 # # TaqI: # [TCGA] # 343 410 # # TatI: # [WGTACW] # 12 741 # # TfiI: # [GAWTC] # 19 128 150 191 265 # # TseI: # [GCWGC] # 33 147 573 # # Tsp45I: # [GTSAC] # 6 244 503 # # Tsp4CI*: # [ACNGT] # 121 250 382 # # TspDTI: # [ATGAA] # 43 91 177 197 245 # # TspEI: # [AATT] # 23 37 39 48 49 72 # 75 121 289 # # TspRI: # [CASTGNN] # 360 393 # # XcmI: # [CCANNNNNNNNNTGG] # 285 468 # # XhoII: # [RGATCY] # 272 481 # # XmnI: # [GAANNNNTTC] # 18 36 128 138 433 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 753 # Reported_sequences: 1 # Reported_hitcount: 211 #---------------------------------------