Restriction Fragments for MRT4/YKL009W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

MRT4/YKL009W on chromosome XI from coordinates 426242 to 426952.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.15861.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 8 11 + DpnI GATC 9 9 . . 8 11 + BstKTI GATC 10 8 . . 8 11 + MboI GATC 7 11 . . 14 19 + AclI AACGTT 15 17 . . 15 18 + TaiI ACGT 18 14 . . 15 18 + SetI ASST 18 14 . . 15 18 + MaeII ACGT 15 17 . . 23 26 + SetI ASST 26 22 . . 23 26 + CviJI RGCY 24 24 . . 23 26 + AluI AGCT 24 24 . . 25 28 + MaeI CTAG 25 27 . . 28 32 + Tsp45I GTSAC 27 32 . . 28 32 + MaeIII GTNAC 27 32 . . 74 79 + ApoI RAATTY 74 78 . . 75 78 + TspEI AATT 74 78 . . 80 83 + TaqI TCGA 80 82 . . 83 87 + TspDTI ATGAA 98 96 . . 90 93 - MnlI CCTC 83 82 . . 96 99 + CviJI RGCY 97 97 . . 99 103 + DdeI CTNAG 99 102 . . 116 119 + TaiI ACGT 119 115 . . 116 119 + SetI ASST 119 115 . . 116 119 + MaeII ACGT 116 118 . . 122 128 + PpuMI RGGWCCY 123 126 . . 122 128 + DraII RGGNCCY 123 126 . . 122 127 + NlaIV GGNNCC 124 124 . . 123 127 + BmgT120I GGNCC 124 125 . . 123 127 + AvaII GGWCC 123 126 . . 123 127 + AsuI* GGNCC 123 126 . . 132 137 + Hpy178III* TCNNGA 133 135 . . 138 142 + Hpy99I CGWCG 142 137 . . 140 143 + TaiI ACGT 143 139 . . 140 143 + SetI ASST 143 139 . . 140 143 + MaeII ACGT 140 142 . . 149 154 - Eco57MI CTGRAG 134 132 . . 149 154 - GsuI CTGGAG 134 132 . . 151 155 - BsrI ACTGG 151 149 . . 167 171 + Hpy188I TCNGA 169 168 . . 181 186 + Cac8I GCNNGC 183 183 . . 184 187 + CviJI RGCY 185 185 . . 188 192 + DdeI CTNAG 188 191 . . 193 196 + MseI TTAA 193 195 . . 195 198 + TspEI AATT 194 198 . . 207 210 - MnlI CCTC 200 199 . . 240 243 - MnlI CCTC 233 232 . . 244 248 + MboII GAAGA 256 255 . . 247 251 + MboII GAAGA 259 258 . . 271 276 + MfeI CAATTG 271 275 . . 272 275 + TspEI AATT 271 275 . . 280 285 + HindIII AAGCTT 280 284 . . 281 284 + SetI ASST 284 280 . . 281 284 + CviJI RGCY 282 282 . . 281 284 + AluI AGCT 282 282 . . 290 293 - AciI CCGC 290 292 . . 295 299 + Tsp45I GTSAC 294 299 . . 295 299 + MaeIII GTNAC 294 299 . . 297 303 + TspRI CASTGNN 303 294 . . 298 302 + BsrI ACTGG 303 301 . . 309 314 + Hpy166II GTNNAC 311 311 . . 312 318 + TspRI CASTGNN 318 309 . . 321 325 + FokI GGATG 334 338 . . 321 325 + BseGI GGATG 327 325 . . 319 322 - MnlI CCTC 312 311 . . 325 330 + Hpy166II GTNNAC 327 327 . . 325 330 + HindII GTYRAC 327 327 . . 330 336 + TspRI CASTGNN 336 327 . . 331 335 + Tsp4CI* ACNGT 333 332 . . 337 347 + FalI AAGNNNNNCTT 328 323 360 355 347 350 + MseI TTAA 347 349 . . 337 347 - FalI AAGNNNNNCTT 360 355 328 323 356 359 + TaiI ACGT 359 355 . . 356 359 + SetI ASST 359 355 . . 356 359 + MaeII ACGT 356 358 . . 364 368 + Hpy188I TCNGA 366 365 . . 373 378 + Hpy178III* TCNNGA 374 376 . . 377 382 + TaqII GACCGA 393 391 . . 408 411 + TspEI AATT 407 411 . . 411 416 + Hpy178III* TCNNGA 412 414 . . 412 418 + SauI* CCTNAGG 413 416 . . 413 417 + DdeI CTNAG 413 416 . . 413 417 - BspCNI CTCAG 405 403 . . 413 417 - BseMII CTCAG 404 402 . . 415 418 - MnlI CCTC 408 407 . . 424 429 + Hpy166II GTNNAC 426 426 . . 424 429 + AccI GTMKAC 425 427 . . 431 436 + BsaAI YACGTR 433 433 . . 431 439 + DraIII CACNNNGTG 436 433 . . 431 436 + PmaCI CACGTG 433 433 . . 432 435 + TaiI ACGT 435 431 . . 432 435 + SetI ASST 435 431 . . 432 435 + MaeII ACGT 432 434 . . 443 448 + ApoI RAATTY 443 447 . . 444 447 + TspEI AATT 443 447 . . 449 454 + PvuII CAGCTG 451 451 . . 449 454 + NspBII* CMGCKG 451 451 . . 450 453 + SetI ASST 453 449 . . 450 453 + CviJI RGCY 451 451 . . 450 453 + AluI AGCT 451 451 . . 452 456 + DdeI CTNAG 452 455 . . 452 456 - BspCNI CTCAG 444 442 . . 452 456 - BseMII CTCAG 443 441 . . 454 457 - MnlI CCTC 447 446 . . 457 461 + MboII GAAGA 469 468 . . 451 457 - Bpu10I CCTNAGC 452 455 . . 451 457 - BbvCI CCTCAGC 452 455 . . 457 466 + XmnI GAANNNNTTC 461 461 . . 471 475 + TfiI GAWTC 471 474 . . 471 475 + HinfI GANTC 471 474 . . 473 477 - TspDTI ATGAA 463 461 . . 466 479 - AjuI GAANNNNNNNTTGG 491 486 459 454 485 488 + CviJI RGCY 486 486 . . 502 507 + ApoI RAATTY 502 506 . . 503 506 + TspEI AATT 502 506 . . 509 514 + ApoI RAATTY 509 513 . . 510 513 + TspEI AATT 509 513 . . 513 518 + MmeI TCCRAC 538 536 . . 528 531 + SetI ASST 531 527 . . 528 531 + CviJI RGCY 529 529 . . 528 531 + AluI AGCT 529 529 . . 537 540 + DpnI GATC 538 538 . . 537 540 + BstKTI GATC 539 537 . . 537 540 + MboI GATC 536 540 . . 539 543 - HphI GGTGA 531 530 . . 548 552 + Tsp4CI* ACNGT 550 549 . . 565 568 + CviRI* TGCA 566 566 . . 567 573 + TspRI CASTGNN 573 564 . . 569 574 + Eco57MI CTGRAG 590 588 . . 569 574 + Eco57I CTGAAG 590 588 . . 571 575 - Hin4II* CCTTC 565 564 . . 581 584 + TspEI AATT 580 584 . . 596 601 + HindIII AAGCTT 596 600 . . 597 600 + SetI ASST 600 596 . . 597 600 + CviJI RGCY 598 598 . . 597 600 + AluI AGCT 598 598 . . 601 604 + MseI TTAA 601 603 . . 609 618 + XmnI GAANNNNTTC 613 613 . . 614 617 + TspEI AATT 613 617 . . 625 629 + BlsI GCNGC 627 626 . . 625 629 + BisI GCNGC 626 627 . . 625 629 + TseI GCWGC 625 628 . . 625 629 - BbvI GCAGC 612 616 . . 631 635 + Hpy188I TCNGA 633 632 . . 620 632 - BarI GAAGNNNNNNTAC 644 639 612 607 634 639 + EcoRI GAATTC 634 638 . . 634 639 + ApoI RAATTY 634 638 . . 635 638 + TspEI AATT 634 638 . . 647 650 + SetI ASST 650 646 . . 654 657 + CviJI RGCY 655 655 . . 654 657 + HaeIII GGCC 655 655 . . 668 678 + BsaXI ACNNNNNCTCC 658 655 688 685 673 676 + SetI ASST 676 672 . . 673 676 + CviJI RGCY 674 674 . . 673 676 + AluI AGCT 674 674 . . 678 684 + TspRI CASTGNN 684 675 . . 679 683 + Tsp4CI* ACNGT 681 680 . . 696 705 + MslI CAYNNNNRTG 700 700 . . 702 705 + FatI CATG 701 705 . . 702 705 + NlaIII CATG 705 701 . . 702 705 + CviAII CATG 702 704 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 286 425 # # AciI: # [CCGC] # 290 421 # # AclI: # [AACGTT] # 15 696 # # AjuI: # [GAANNNNNNNTTGG] # 32 220 459 # # AluI: # [AGCT] # 24 37 69 76 78 169 # 258 # # ApoI: # [RAATTY] # 7 59 74 77 125 369 # # AsuI*: # [GGNCC] # 123 588 # # AvaII: # [GGWCC] # 123 588 # # BarI: # [GAAGNNNNNNTAC] # 32 67 612 # # BbvCI: # [CCTCAGC] # 259 452 # # BbvI: # [GCAGC] # 99 612 # # BisI: # [GCNGC] # 85 626 # # BlsI: # [GCNGC] # 84 627 # # BmgT120I: # [GGNCC] # 124 587 # # Bpu10I: # [CCTNAGC] # 259 452 # # BsaAI: # [YACGTR] # 278 433 # # BsaXI: # [ACNNNNNCTCC] # 23 30 658 # # BseGI: # [GGATG] # 327 384 # # BseMII: # [CTCAG] # 39 268 404 # # BspCNI: # [CTCAG] # 39 267 405 # # BsrI: # [ACTGG] # 151 152 408 # # BstKTI: # [GATC] # 10 172 529 # # Cac8I: # [GCNNGC] # 183 528 # # CviAII: # [CATG] # 9 702 # # CviJI: # [RGCY] # 19 24 35 37 43 57 # 69 73 88 97 169 # # CviRI*: # [TGCA] # 145 566 # # DdeI: # [CTNAG] # 39 89 99 225 259 # # DpnI: # [GATC] # 9 173 529 # # DraII: # [RGGNCCY] # 123 588 # # DraIII: # [CACNNNGTG] # 275 436 # # Eco57I: # [CTGAAG] # 121 590 # # Eco57MI: # [CTGRAG] # 121 134 456 # # EcoRI: # [GAATTC] # 77 634 # # FalI: # [AAGNNNNNCTT] # 0 0 32 328 351 # # FatI: # [CATG] # 10 701 # # FokI: # [GGATG] # 334 377 # # GsuI: # [CTGGAG] # 134 577 # # HaeIII: # [GGCC] # 56 655 # # Hin4II*: # [CCTTC] # 146 565 # # HindII: # [GTYRAC] # 327 384 # # HindIII: # [AAGCTT] # 115 280 316 # # HinfI: # [GANTC] # 240 471 # # HphI: # [GGTGA] # 180 531 # # Hpy166II: # [GTNNAC] # 16 99 285 311 # # Hpy178III*: # [TCNNGA] # 38 133 241 299 # # Hpy188I: # [TCNGA] # 78 169 197 267 # # Hpy99I: # [CGWCG] # 142 569 # # MaeI: # [CTAG] # 25 686 # # MaeII: # [ACGT] # 15 24 76 101 216 279 # # MaeIII: # [GTNAC] # 27 267 417 # # MboI: # [GATC] # 7 175 529 # # MboII: # [GAAGA] # 3 210 242 256 # # MfeI: # [CAATTG] # 271 440 # # MmeI: # [TCCRAC] # 173 538 # # MnlI: # [CCTC] # 33 39 79 83 96 117 # 264 # # MseI: # [TTAA] # 110 154 193 254 # # MslI: # [CAYNNNNRTG] # 11 700 # # NlaIII: # [CATG] # 6 705 # # NlaIV: # [GGNNCC] # 124 587 # # NspBII*: # [CMGCKG] # 260 451 # # PmaCI: # [CACGTG] # 278 433 # # PpuMI: # [RGGWCCY] # 123 588 # # PvuII: # [CAGCTG] # 260 451 # # SauI*: # [CCTNAGG] # 298 413 # # SetI: # [ASST] # 8 18 18 24 26 35 # 50 69 75 76 78 93 # 141 # # TaiI: # [ACGT] # 18 24 76 101 216 276 # # TaqI: # [TCGA] # 80 631 # # TaqII: # [GACCGA] # 318 393 # # TfiI: # [GAWTC] # 240 471 # # TseI: # [GCWGC] # 86 625 # # Tsp45I: # [GTSAC] # 27 267 417 # # Tsp4CI*: # [ACNGT] # 30 131 217 333 # # TspDTI: # [ATGAA] # 98 248 365 # # TspEI: # [AATT] # 7 21 33 36 59 71 # 74 77 77 120 136 # # TspRI: # [CASTGNN] # 15 18 27 111 237 303 # # XmnI: # [GAANNNNTTC] # 98 152 461 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 711 # Reported_sequences: 1 # Reported_hitcount: 159 #---------------------------------------