Restriction Fragments for YJRCdelta15



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The currently selected gene/sequence is :

YJRCdelta15 on chromosome X from coordinates 531675 to 531516.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.5536.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 23 28 + BsrDI GCAATG 30 28 . . 28 32 + HphI GGTGA 40 39 . . 29 33 + Tsp45I GTSAC 28 33 . . 29 33 + MaeIII GTNAC 28 33 . . 47 51 + MboII GAAGA 59 58 . . 52 55 + TspEI AATT 51 55 . . 55 58 + CviRI* TGCA 56 56 . . 56 67 - BcgI CGANNNNNNTGC 79 77 45 43 72 76 + TfiI GAWTC 72 75 . . 72 76 + HinfI GANTC 72 75 . . 76 79 + FatI CATG 75 79 . . 76 79 + NlaIII CATG 79 75 . . 76 79 + CviAII CATG 76 78 . . 92 96 + TfiI GAWTC 92 95 . . 92 96 + HinfI GANTC 92 95 . . 103 107 + Hpy99I CGWCG 107 102 . . 115 118 + SetI ASST 118 114 . . 116 119 + MnlI CCTC 126 125 . . 131 136 + Hpy166II GTNNAC 133 133 . . 135 138 + SetI ASST 138 134 . . 143 148 + SfeI* CTRYAG 143 147 . . 147 150 + SetI ASST 150 146 . . 147 150 + CviJI RGCY 148 148 . . 147 150 + AluI AGCT 148 148 . . #--------------------------------------- # # # AluI: # [AGCT] # 12 148 # # BcgI: # [CGANNNNNNTGC] # 34 45 81 # # BsrDI: # [GCAATG] # 30 130 # # CviAII: # [CATG] # 76 84 # # CviJI: # [RGCY] # 12 148 # # CviRI*: # [TGCA] # 56 104 # # FatI: # [CATG] # 75 85 # # HinfI: # [GANTC] # 20 68 72 # # HphI: # [GGTGA] # 40 120 # # Hpy166II: # [GTNNAC] # 27 133 # # Hpy99I: # [CGWCG] # 53 107 # # MaeIII: # [GTNAC] # 28 132 # # MboII: # [GAAGA] # 59 101 # # MnlI: # [CCTC] # 34 126 # # NlaIII: # [CATG] # 79 81 # # SetI: # [ASST] # 10 12 20 118 # # SfeI*: # [CTRYAG] # 17 143 # # TfiI: # [GAWTC] # 20 68 72 # # Tsp45I: # [GTSAC] # 28 132 # # TspEI: # [AATT] # 51 109 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 160 # Reported_sequences: 1 # Reported_hitcount: 24 #---------------------------------------