Restriction Fragments for THI11/YJR156C



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

THI11/YJR156C on chromosome X from coordinates 729596 to 728574.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.32415.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 3 8 + Hpy166II GTNNAC 5 5 . . 3 8 + AccI GTMKAC 4 6 . . 5 10 + SfeI* CTRYAG 5 9 . . 15 18 + DpnI GATC 16 16 . . 15 18 + BstKTI GATC 17 15 . . 15 18 + MboI GATC 14 18 . . 32 36 + BsrI ACTGG 37 35 . . 60 63 + TspEI AATT 59 63 . . 69 72 + CviJI RGCY 70 70 . . 81 84 + SetI ASST 84 80 . . 83 87 + MaeIII GTNAC 82 87 . . 77 90 - AjuI GAANNNNNNNTTGG 102 97 70 65 99 102 + SetI ASST 102 98 . . 102 107 + XbaI TCTAGA 102 106 . . 102 107 + Hpy178III* TCNNGA 103 105 . . 103 106 + MaeI CTAG 103 105 . . 108 113 - BsrDI GCAATG 107 105 . . 113 117 + BccI CCATC 121 122 . . 114 118 - FokI GGATG 100 104 . . 114 118 - BseGI GGATG 113 111 . . 118 121 + MaeI CTAG 118 120 . . 133 137 + Hin4II* CCTTC 143 142 . . 136 140 + Hpy188I TCNGA 138 137 . . 124 137 - AjuI GAANNNNNNNTTGG 149 144 117 112 138 142 + FokI GGATG 151 155 . . 138 142 + BseGI GGATG 144 142 . . 142 146 + Tsp45I GTSAC 141 146 . . 142 146 + MaeIII GTNAC 141 146 . . 144 150 + TspRI CASTGNN 150 141 . . 146 150 + DdeI CTNAG 146 149 . . 146 150 - BspCNI CTCAG 138 136 . . 146 150 - BseMII CTCAG 137 135 . . 151 154 + MseI TTAA 151 153 . . 153 156 + TspEI AATT 152 156 . . 157 162 + XhoII RGATCY 157 161 . . 157 161 + BinI* GGATC 165 166 . . 158 161 + DpnI GATC 159 159 . . 158 161 + BstKTI GATC 160 158 . . 158 161 + MboI GATC 157 161 . . 167 170 + SetI ASST 170 166 . . 169 174 + SalI GTCGAC 169 173 . . 169 174 + Hpy166II GTNNAC 171 171 . . 169 174 + HindII GTYRAC 171 171 . . 169 174 + AccI GTMKAC 170 172 . . 170 173 + TaqI TCGA 170 172 . . 174 177 + FatI CATG 173 177 . . 174 177 + NlaIII CATG 177 173 . . 174 177 + CviAII CATG 174 176 . . 186 189 + CviJI RGCY 187 187 . . 189 192 + FatI CATG 188 192 . . 189 192 + NlaIII CATG 192 188 . . 189 192 + CviAII CATG 189 191 . . 192 195 + DpnI GATC 193 193 . . 192 195 + BstKTI GATC 194 192 . . 192 195 + MboI GATC 191 195 . . 195 205 + BsiYI* CCNNNNNNNGG 201 198 . . 195 205 + PflMI CCANNNNNTGG 201 198 . . 192 196 - BinI* GGATC 186 187 . . 199 202 + SetI ASST 202 198 . . 200 205 + StyI CCWWGG 200 204 . . 200 205 + SecI* CCNNGG 200 204 . . 204 207 + CviJI RGCY 205 205 . . 205 215 + MwoI GCNNNNNNNGC 211 208 . . 205 209 + BlsI GCNGC 207 206 . . 205 209 + BisI GCNGC 206 207 . . 205 209 + TseI GCWGC 205 208 . . 209 214 + StyI CCWWGG 209 213 . . 209 214 + SecI* CCNNGG 209 213 . . 205 209 - BbvI GCAGC 192 196 . . 210 221 + CspCI CAANNNNNGTGG 198 196 233 231 213 216 + CviJI RGCY 214 214 . . 213 216 + HaeIII GGCC 214 214 . . 213 217 + BmgT120I GGNCC 214 215 . . 213 217 + AsuI* GGNCC 213 216 . . 216 221 + DsaI* CCRYGG 216 220 . . 216 221 + SecI* CCNNGG 216 220 . . 227 233 + TspRI CASTGNN 233 224 . . 229 233 + Tsp45I GTSAC 228 233 . . 229 233 + MaeIII GTNAC 228 233 . . 226 230 - BsrI ACTGG 226 224 . . 225 230 - BfiI ACTGGG 220 219 . . 232 235 + SetI ASST 235 231 . . 233 236 + MnlI CCTC 243 242 . . 242 245 + MnlI CCTC 252 251 . . 249 254 - MmeI TCCRAC 230 228 . . 259 269 + BsiYI* CCNNNNNNNGG 265 262 . . 264 271 + SgrAI CRCCGGYG 265 269 . . 265 270 + Cfr10I RCCGGY 265 269 . . 265 270 + BetI* WCCGGW 265 269 . . 265 270 + AgeI ACCGGT 265 269 . . 266 269 + HpaII CCGG 266 268 . . 263 267 - HphI GGTGA 255 254 . . 275 280 + TatI WGTACW 275 279 . . 276 279 + Csp6I GTAC 276 278 . . 276 279 + RsaI GTAC 277 277 . . 280 283 + MseI TTAA 280 282 . . 287 292 + BtsI GCAGTG 294 292 . . 288 294 + TspRI CASTGNN 294 285 . . 297 303 + TspRI CASTGNN 303 294 . . 299 304 + Eco57MI CTGRAG 320 318 . . 299 304 + Eco57I CTGAAG 320 318 . . 301 305 + MboII GAAGA 313 312 . . 301 306 + BbvII* GAAGAC 308 312 . . 304 314 + Hin4I GAYNNNNNVTC 295 290 327 322 304 314 + Eam1105I GACNNNNNGTC 309 308 . . 309 313 - BsrI ACTGG 309 307 . . 304 314 - Hin4I GAYNNNNNVTC 327 322 295 290 312 316 - BslFI GGGAC 297 301 . . 327 331 + MboII GAAGA 339 338 . . 327 339 + BarI GAAGNNNNNNTAC 319 314 351 346 330 333 + DpnI GATC 331 331 . . 330 333 + BstKTI GATC 332 330 . . 330 333 + MboI GATC 329 333 . . 335 339 + MaeIII GTNAC 334 339 . . 338 341 + TaiI ACGT 341 337 . . 338 341 + SetI ASST 341 337 . . 338 341 + MaeII ACGT 338 340 . . 343 347 + HphI GGTGA 355 354 . . 346 351 + EcoRI GAATTC 346 350 . . 346 351 + ApoI RAATTY 346 350 . . 347 350 + TspEI AATT 346 350 . . 356 361 + XhoII RGATCY 356 360 . . 357 360 + DpnI GATC 358 358 . . 357 360 + BstKTI GATC 359 357 . . 357 366 + BsaBI GATNNNNATC 361 361 . . 357 360 + MboI GATC 356 360 . . 357 361 - BinI* GGATC 351 352 . . 364 369 + ClaI ATCGAT 365 367 . . 365 368 + TaqI TCGA 365 367 . . 368 372 + TspDTI ATGAA 383 381 . . 371 374 + TspEI AATT 370 374 . . 386 390 + Tsp4CI* ACNGT 388 387 . . 391 395 + TspDTI ATGAA 406 404 . . 395 398 + CviJI RGCY 396 396 . . 400 404 + MboII GAAGA 412 411 . . 400 405 + BbvII* GAAGAC 407 411 . . 408 414 + TspRI CASTGNN 414 405 . . 408 413 - BtsI GCAGTG 407 405 . . 410 415 - EcoP15I CAGCAG 382 384 . . 416 420 + Hpy188I TCNGA 418 417 . . 427 431 + TspDTI ATGAA 442 440 . . 440 445 + TatI WGTACW 440 444 . . 441 444 + Csp6I GTAC 441 443 . . 441 444 + RsaI GTAC 442 442 . . 449 452 + TaqI TCGA 449 451 . . 451 455 + MboII GAAGA 463 462 . . 463 467 - SfaNI GCATC 453 457 . . 479 482 + TaqI TCGA 479 481 . . 488 491 + CviRI* TGCA 489 489 . . 497 500 + TaqI TCGA 497 499 . . 500 503 + TspEI AATT 499 503 . . 505 509 + MboII GAAGA 517 516 . . 509 514 + TatI WGTACW 509 513 . . 509 514 + ScaI AGTACT 511 511 . . 510 513 + Csp6I GTAC 510 512 . . 505 510 - Ksp632I* CTCTTC 500 503 . . 510 513 + RsaI GTAC 511 511 . . 515 520 + BalI TGGCCA 517 517 . . 515 520 + CfrI YGGCCR 515 519 . . 516 519 + CviJI RGCY 517 517 . . 516 519 + HaeIII GGCC 517 517 . . 533 541 + AlwNI CAGNNNCTG 538 535 . . 534 537 + SetI ASST 537 533 . . 534 537 + CviJI RGCY 535 535 . . 534 537 + AluI AGCT 535 535 . . 535 545 + MwoI GCNNNNNNNGC 541 538 . . 538 542 + Hpy188I TCNGA 540 539 . . 541 545 - SfaNI GCATC 531 535 . . 558 561 + TspEI AATT 557 561 . . 570 573 + CviJI RGCY 571 571 . . 571 576 + Cac8I GCNNGC 573 573 . . 575 585 + MwoI GCNNNNNNNGC 581 578 . . 596 599 + Csp6I GTAC 596 598 . . 596 599 + RsaI GTAC 597 597 . . 598 602 + Tsp4CI* ACNGT 600 599 . . 613 616 + CviRI* TGCA 614 614 . . 620 624 + TspDTI ATGAA 635 633 . . 622 627 + ApoI RAATTY 622 626 . . 623 626 + TspEI AATT 622 626 . . 630 634 + MboII GAAGA 642 641 . . 639 649 + BsiYI* CCNNNNNNNGG 645 642 . . 647 650 + SetI ASST 650 646 . . 650 654 + Hpy188I TCNGA 652 651 . . 659 664 + Hpy178III* TCNNGA 660 662 . . 662 673 + BdaI TGANNNNNNTCA 651 649 685 683 666 669 + CviJI RGCY 667 667 . . 668 672 + BccI CCATC 676 677 . . 671 676 + Hpy178III* TCNNGA 672 674 . . 662 673 - BdaI TGANNNNNNTCA 685 683 651 649 675 679 - Hin4II* CCTTC 669 668 . . 689 692 + TaiI ACGT 692 688 . . 689 692 + SetI ASST 692 688 . . 689 692 + MaeII ACGT 689 691 . . 694 697 + MaeI CTAG 694 696 . . 696 699 + CviJI RGCY 697 697 . . 702 712 + BsiYI* CCNNNNNNNGG 708 705 . . 711 714 + CviJI RGCY 712 712 . . 708 712 - Hin4II* CCTTC 702 701 . . 728 731 + TaqI TCGA 728 730 . . 737 740 + CviJI RGCY 738 738 . . 739 742 + MnlI CCTC 749 748 . . 742 747 + MfeI CAATTG 742 746 . . 743 746 + TspEI AATT 742 746 . . 754 757 + DpnI GATC 755 755 . . 754 757 + BstKTI GATC 756 754 . . 754 757 + MboI GATC 753 757 . . 782 786 + MaeIII GTNAC 781 786 . . 795 800 + Ksp632I* CTCTTC 801 804 . . 796 800 - MboII GAAGA 788 787 . . 798 802 - TspDTI ATGAA 788 786 . . 806 811 + TatI WGTACW 806 810 . . 806 811 + Bsp1407I TGTACA 806 810 . . 807 810 + Csp6I GTAC 807 809 . . 807 810 + RsaI GTAC 808 808 . . 814 819 + Hpy166II GTNNAC 816 816 . . 818 822 + Tsp4CI* ACNGT 820 819 . . 816 820 - HphI GGTGA 808 807 . . 821 825 + Tsp45I GTSAC 820 825 . . 821 825 + MaeIII GTNAC 820 825 . . 824 828 + BsrI ACTGG 829 827 . . 828 832 + MboII GAAGA 840 839 . . 833 836 + SetI ASST 836 832 . . 834 840 + BstEII GGTNACC 834 839 . . 835 839 + MaeIII GTNAC 834 839 . . 831 835 - Hin4II* CCTTC 825 824 . . 838 843 + Cfr10I RCCGGY 838 842 . . 838 843 + BetI* WCCGGW 838 842 . . 838 843 + AgeI ACCGGT 838 842 . . 839 842 + HpaII CCGG 839 841 . . 842 846 + MaeIII GTNAC 841 846 . . 845 849 + Tsp4CI* ACNGT 847 846 . . 857 862 + TsoI TARCCA 873 871 . . 858 861 + CviJI RGCY 859 859 . . 874 884 + Hin4I GAYNNNNNVTC 865 860 897 892 874 882 + Tth111I GACNNNGTC 877 878 . . 880 884 + BsmAI GTCTC 885 889 . . 881 886 + Hpy178III* TCNNGA 882 884 . . 883 886 + TaqI TCGA 883 885 . . 896 900 + TspDTI ATGAA 911 909 . . 908 912 + BssKI CCNGG 907 912 . . 908 912 + BseBI CCWGG 909 910 . . 908 912 + ScrFI CCNGG 909 910 . . 908 912 + EcoRII CCWGG 907 912 . . 910 915 + BalI TGGCCA 912 912 . . 910 915 + CfrI YGGCCR 910 914 . . 911 914 + CviJI RGCY 912 912 . . 911 914 + HaeIII GGCC 912 912 . . 922 926 + MboII GAAGA 934 933 . . 926 929 + SetI ASST 929 925 . . 922 927 - Ksp632I* CTCTTC 917 920 . . 925 928 - MnlI CCTC 918 917 . . 931 935 + Hpy188I TCNGA 933 932 . . 934 937 + DpnI GATC 935 935 . . 934 937 + BstKTI GATC 936 934 . . 934 937 + MboI GATC 933 937 . . 934 938 - BinI* GGATC 928 929 . . 945 948 + SetI ASST 948 944 . . 945 948 + CviJI RGCY 946 946 . . 945 948 + AluI AGCT 946 946 . . 960 963 + CviJI RGCY 961 961 . . 957 961 - BccI CCATC 951 952 . . 959 964 - TsoI TARCCA 949 947 . . 965 969 - TspDTI ATGAA 955 953 . . 969 974 + Hpy178III* TCNNGA 970 972 . . 979 982 + CviRI* TGCA 980 980 . . 990 993 + SetI ASST 993 989 . . 988 992 - Hin4II* CCTTC 982 981 . . 992 995 + Csp6I GTAC 992 994 . . 992 995 + RsaI GTAC 993 993 . . 998 1003 + Hpy178III* TCNNGA 999 1001 . . 1002 1012 + BplI GAGNNNNNCTC 993 988 . . 1008 1011 + CviJI RGCY 1009 1009 . . 1009 1019 + MwoI GCNNNNNNNGC 1015 1012 . . 1009 1015 + SapI GCTCTTC 1016 1019 . . 1010 1015 + Ksp632I* CTCTTC 1016 1019 . . 1011 1015 - MboII GAAGA 1003 1002 . . 1017 1020 + SetI ASST 1020 1016 . . 1017 1020 + CviJI RGCY 1018 1018 . . 1017 1020 + AluI AGCT 1018 1018 . . 1020 1023 + MseI TTAA 1020 1022 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 4 166 853 # # AgeI: # [ACCGGT] # 185 265 573 # # AjuI: # [GAANNNNNNNTTGG] # 15 32 32 70 874 # # AluI: # [AGCT] # 5 72 411 535 # # AlwNI: # [CAGNNNCTG] # 485 538 # # ApoI: # [RAATTY] # 276 346 401 # # AsuI*: # [GGNCC] # 213 810 # # BalI: # [TGGCCA] # 111 395 517 # # BarI: # [GAAGNNNNNNTAC] # 32 319 672 # # BbvI: # [GCAGC] # 192 831 # # BbvII*: # [GAAGAC] # 99 308 616 # # BccI: # [CCATC] # 72 121 275 555 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 338 651 # # BetI*: # [WCCGGW] # 185 265 573 # # BfiI: # [ACTGGG] # 220 803 # # BinI*: # [GGATC] # 21 95 165 165 577 # # BisI: # [GCNGC] # 206 817 # # BlsI: # [GCNGC] # 207 816 # # BmgT120I: # [GGNCC] # 214 809 # # BplI: # [GAGNNNNNCTC] # 30 993 # # BsaBI: # [GATNNNNATC] # 361 662 # # BseBI: # [CCWGG] # 114 909 # # BseGI: # [GGATG] # 31 113 879 # # BseMII: # [CTCAG] # 137 886 # # BsiYI*: # [CCNNNNNNNGG] # 63 64 201 315 380 # # BslFI: # [GGGAC] # 297 726 # # BsmAI: # [GTCTC] # 138 885 # # Bsp1407I: # [TGTACA] # 217 806 # # BspCNI: # [CTCAG] # 138 885 # # BsrDI: # [GCAATG] # 107 916 # # BsrI: # [ACTGG] # 37 83 189 194 520 # # BssKI: # [CCNGG] # 116 907 # # BstEII: # [GGTNACC] # 189 834 # # BstKTI: # [GATC] # 17 27 34 87 138 143 # 180 397 # # BtsI: # [GCAGTG] # 113 294 616 # # Cac8I: # [GCNNGC] # 450 573 # # Cfr10I: # [RCCGGY] # 185 265 573 # # CfrI: # [YGGCCR] # 113 395 515 # # ClaI: # [ATCGAT] # 365 658 # # Csp6I: # [GTAC] # 31 69 86 165 185 211 # 276 # # CspCI: # [CAANNNNNGTGG] # 35 198 790 # # CviAII: # [CATG] # 15 174 834 # # CviJI: # [RGCY] # 5 9 9 15 15 18 # 18 26 30 34 36 48 # 53 70 96 117 121 121 # 182 # # CviRI*: # [TGCA] # 43 125 366 489 # # DdeI: # [CTNAG] # 146 877 # # DpnI: # [GATC] # 16 27 34 88 138 143 # 180 397 # # DsaI*: # [CCRYGG] # 216 807 # # Eam1105I: # [GACNNNNNGTC] # 309 714 # # Eco57I: # [CTGAAG] # 320 703 # # Eco57MI: # [CTGRAG] # 320 703 # # EcoP15I: # [CAGCAG] # 382 641 # # EcoRI: # [GAATTC] # 346 677 # # EcoRII: # [CCWGG] # 116 907 # # FatI: # [CATG] # 15 173 835 # # FokI: # [GGATG] # 51 100 872 # # HaeIII: # [GGCC] # 111 214 303 395 # # Hin4I: # [GAYNNNNNVTC] # 0 0 32 32 126 295 # 538 # # Hin4II*: # [CCTTC] # 33 41 123 143 157 526 # # HindII: # [GTYRAC] # 171 852 # # HpaII: # [CCGG] # 184 266 573 # # HphI: # [GGTGA] # 100 215 255 453 # # Hpy166II: # [GTNNAC] # 5 166 207 645 # # Hpy178III*: # [TCNNGA] # 12 24 29 88 103 210 # 557 # # Hpy188I: # [TCNGA] # 90 112 122 138 280 281 # # Ksp632I*: # [CTCTTC] # 7 99 116 301 500 # # MaeI: # [CTAG] # 15 103 329 576 # # MaeII: # [ACGT] # 334 338 351 # # MaeIII: # [GTNAC] # 7 14 39 59 82 87 # 106 182 447 # # MboI: # [GATC] # 14 27 34 90 138 143 # 180 397 # # MboII: # [GAAGA] # 20 26 51 52 54 69 # 73 94 125 146 313 # # MfeI: # [CAATTG] # 281 742 # # MmeI: # [TCCRAC] # 230 793 # # MnlI: # [CCTC] # 9 105 169 243 497 # # MseI: # [TTAA] # 3 129 151 740 # # MwoI: # [GCNNNNNNNGC] # 8 40 211 330 434 # # NlaIII: # [CATG] # 15 177 831 # # PflMI: # [CCANNNNNTGG] # 201 822 # # RsaI: # [GTAC] # 30 69 86 165 185 211 # 277 # # SalI: # [GTCGAC] # 169 854 # # SapI: # [GCTCTTC] # 7 1016 # # ScaI: # [AGTACT] # 511 512 # # ScrFI: # [CCNGG] # 114 909 # # SecI*: # [CCNNGG] # 7 9 200 807 # # SetI: # [ASST] # 3 18 19 27 32 33 # 42 45 68 84 93 106 # 113 144 196 # # SfaNI: # [GCATC] # 78 453 492 # # SfeI*: # [CTRYAG] # 5 1018 # # SgrAI: # [CRCCGGYG] # 265 758 # # StyI: # [CCWWGG] # 9 200 814 # # TaiI: # [ACGT] # 331 341 351 # # TaqI: # [TCGA] # 18 30 84 140 155 170 # 195 231 # # TatI: # [WGTACW] # 69 165 217 275 297 # # TseI: # [GCWGC] # 205 818 # # TsoI: # [TARCCA] # 74 76 873 # # Tsp45I: # [GTSAC] # 87 141 203 592 # # Tsp4CI*: # [ACNGT] # 27 176 212 220 388 # # TspDTI: # [ATGAA] # 23 36 44 68 123 153 # 193 383 # # TspEI: # [AATT] # 24 58 59 65 93 120 # 129 194 281 # # TspRI: # [CASTGNN] # 9 61 83 111 150 609 # # Tth111I: # [GACNNNGTC] # 146 877 # # XbaI: # [TCTAGA] # 102 921 # # XhoII: # [RGATCY] # 157 199 667 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1023 # Reported_sequences: 1 # Reported_hitcount: 280 #---------------------------------------