Restriction Fragments for DAN4/YJR151C



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The currently selected gene/sequence is :

DAN4/YJR151C on chromosome X from coordinates 715740 to 712255.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.26830.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 5 8 + MseI TTAA 5 7 . . 14 18 + SfaNI GCATC 23 27 . . 27 30 + TspEI AATT 26 30 . . 33 36 + CviJI RGCY 34 34 . . 35 41 + Bpu10I CCTNAGC 36 39 . . 36 40 + DdeI CTNAG 36 39 . . 39 42 + SetI ASST 42 38 . . 39 42 + CviJI RGCY 40 40 . . 39 42 + AluI AGCT 40 40 . . 40 50 + MwoI GCNNNNNNNGC 46 43 . . 43 48 + SpeI ACTAGT 43 47 . . 44 47 + MaeI CTAG 44 46 . . 49 53 + TauI GCSGC 52 49 . . 49 53 + BlsI GCNGC 51 50 . . 49 53 + BisI GCNGC 50 51 . . 51 54 + CviJI RGCY 52 52 . . 52 56 + BlsI GCNGC 54 53 . . 52 56 + BisI GCNGC 53 54 . . 52 56 + TseI GCWGC 52 55 . . 49 52 - AciI CCGC 49 51 . . 55 65 + MwoI GCNNNNNNNGC 61 58 . . 52 56 - BbvI GCAGC 39 43 . . 73 83 + BsaXI ACNNNNNCTCC 63 60 93 90 89 93 + TspDTI ATGAA 104 102 . . 95 99 + PleI GAGTC 103 104 . . 95 99 + MlyI GAGTC 104 104 . . 95 99 + HinfI GANTC 95 98 . . 97 102 + Hpy166II GTNNAC 99 99 . . 97 102 + HindII GTYRAC 99 99 . . 101 104 + SetI ASST 104 100 . . 110 113 + TspEI AATT 109 113 . . 114 117 + CviJI RGCY 115 115 . . 127 131 + Hpy188I TCNGA 129 128 . . 134 138 + Hpy188I TCNGA 136 135 . . 137 142 + SduI GDGCHC 141 137 . . 137 142 + HgiJII* GRGCYC 141 137 . . 138 141 + CviJI RGCY 139 139 . . 152 157 + TatI WGTACW 152 156 . . 152 157 + ScaI AGTACT 154 154 . . 153 156 + Csp6I GTAC 153 155 . . 153 156 + RsaI GTAC 154 154 . . 164 169 + Hpy178III* TCNNGA 165 167 . . 164 169 + BetI* WCCGGW 164 168 . . 164 169 + BspMII* TCCGGA 164 168 . . 165 168 + HpaII CCGG 165 167 . . 168 172 + TfiI GAWTC 168 171 . . 168 172 + HinfI GANTC 168 171 . . 171 175 - HphI GGTGA 163 162 . . 193 197 + Hin4II* CCTTC 203 202 . . 196 200 + Hpy188I TCNGA 198 197 . . 205 209 + BlsI GCNGC 207 206 . . 205 209 + BisI GCNGC 206 207 . . 205 209 + BbvI GCAGC 217 221 . . 205 209 + TseI GCWGC 205 208 . . 206 211 + EcoP15I CAGCAG 236 238 . . 208 212 + BlsI GCNGC 210 209 . . 208 212 + BisI GCNGC 209 210 . . 208 212 + BbvI GCAGC 220 224 . . 208 212 + TseI GCWGC 208 211 . . 210 213 + CviJI RGCY 211 211 . . 211 215 - BceAI ACGGC 196 198 . . 224 228 + Tsp4CI* ACNGT 226 225 . . 226 230 + HphI GGTGA 238 237 . . 240 243 + TaqI TCGA 240 242 . . 247 252 + Cfr10I RCCGGY 247 251 . . 247 252 + BetI* WCCGGW 247 251 . . 247 252 + AgeI ACCGGT 247 251 . . 248 251 + HpaII CCGG 248 250 . . 259 263 + HphI GGTGA 271 270 . . 263 267 + TspDTI ATGAA 278 276 . . 268 272 + MaeIII GTNAC 267 272 . . 272 275 + MaeI CTAG 272 274 . . 278 283 + BclI TGATCA 278 282 . . 279 282 + DpnI GATC 280 280 . . 279 282 + BstKTI GATC 281 279 . . 279 282 + MboI GATC 278 282 . . 282 293 + TstI CACNNNNNNTCC 273 268 305 300 282 288 + TspRI CASTGNN 288 279 . . 283 287 + BsrI ACTGG 288 286 . . 292 297 + DsaI* CCRYGG 292 296 . . 292 297 + SecI* CCNNGG 292 296 . . 291 295 - TspGWI ACGGA 281 279 . . 297 300 + Csp6I GTAC 297 299 . . 297 300 + RsaI GTAC 298 298 . . 310 313 + MseI TTAA 310 312 . . 314 317 + CviJI RGCY 315 315 . . 315 320 + NaeI GCCGGC 317 317 . . 315 320 + MroNI GCCGGC 315 319 . . 315 320 + Cac8I GCNNGC 317 317 . . 315 320 + Cfr10I RCCGGY 315 319 . . 316 319 + HpaII CCGG 316 318 . . 318 321 + CviJI RGCY 319 319 . . 324 329 + Ksp632I* CTCTTC 330 333 . . 325 329 - MboII GAAGA 317 316 . . 330 335 + HaeII RGCGCY 334 330 . . 330 335 + Eco47III AGCGCT 332 332 . . 331 334 + Hin6I GCGC 331 333 . . 331 334 + GlaI GCGC 332 332 . . 326 331 - Eco57MI CTGRAG 311 309 . . 331 334 + HhaI GCGC 333 331 . . 326 331 - Eco57I CTGAAG 311 309 . . 342 346 + FokI GGATG 355 359 . . 342 346 + BseGI GGATG 348 346 . . 343 347 - BccI CCATC 337 338 . . 355 359 + BceAI ACGGC 371 373 . . 357 360 + CviJI RGCY 358 358 . . 367 370 + SetI ASST 370 366 . . 368 371 + MnlI CCTC 378 377 . . 364 377 - AjuI GAANNNNNNNTTGG 389 384 357 352 395 398 + MaeI CTAG 395 397 . . 397 402 + TatI WGTACW 397 401 . . 397 402 + ScaI AGTACT 399 399 . . 398 401 + Csp6I GTAC 398 400 . . 398 401 + RsaI GTAC 399 399 . . 403 406 + TaqI TCGA 403 405 . . 448 451 + TaqI TCGA 448 450 . . 463 466 + SetI ASST 466 462 . . 464 467 + MnlI CCTC 474 473 . . 502 505 + TaqI TCGA 502 504 . . 517 520 + SetI ASST 520 516 . . 518 521 + MnlI CCTC 528 527 . . 556 559 + TaqI TCGA 556 558 . . 571 574 + SetI ASST 574 570 . . 572 575 + MnlI CCTC 582 581 . . 610 613 + TaqI TCGA 610 612 . . 625 628 + SetI ASST 628 624 . . 626 629 + MnlI CCTC 636 635 . . 706 709 + SetI ASST 709 705 . . 707 710 + MnlI CCTC 717 716 . . 736 739 + TaqI TCGA 736 738 . . 763 766 + TaqI TCGA 763 765 . . 805 808 + SetI ASST 808 804 . . 806 809 + MnlI CCTC 816 815 . . 812 815 + AciI CCGC 812 814 . . 812 817 + BsrBI CCGCTC 814 814 . . 841 844 + SetI ASST 844 840 . . 842 845 + MnlI CCTC 852 851 . . 866 869 + AciI CCGC 866 868 . . 866 871 + BsrBI CCGCTC 868 868 . . 877 880 + SetI ASST 880 876 . . 878 881 + MnlI CCTC 888 887 . . 901 906 + TatI WGTACW 901 905 . . 901 906 + ScaI AGTACT 903 903 . . 902 905 + Csp6I GTAC 902 904 . . 902 905 + RsaI GTAC 903 903 . . 908 911 + MaeI CTAG 908 910 . . 914 917 + MnlI CCTC 924 923 . . 918 921 + CviRI* TGCA 919 919 . . 919 923 + SfaNI GCATC 928 932 . . 923 926 + MnlI CCTC 933 932 . . 920 924 - FokI GGATG 906 910 . . 920 924 - BseGI GGATG 919 917 . . 939 945 + TspRI CASTGNN 945 936 . . 939 944 - BtsI GCAGTG 938 936 . . 949 952 + SetI ASST 952 948 . . 950 953 + MnlI CCTC 960 959 . . 965 969 - BsrI ACTGG 965 963 . . 977 983 + AarI CACCTGC 987 991 . . 978 981 + SetI ASST 981 977 . . 978 983 + BspMI ACCTGC 987 991 . . 991 995 + BceAI ACGGC 1007 1009 . . 993 996 + CviJI RGCY 994 994 . . 1012 1015 + SetI ASST 1015 1011 . . 1013 1016 + MnlI CCTC 1023 1022 . . 1016 1019 + MaeI CTAG 1016 1018 . . 1021 1025 + BsmAI GTCTC 1026 1030 . . 1036 1041 - BsmI GAATGC 1036 1034 . . 1050 1053 + CviRI* TGCA 1051 1051 . . 1096 1101 + GsaI CCCAGC 1100 1096 . . 1096 1101 + BseYI CCCAGC 1096 1100 . . 1094 1098 - BslFI GGGAC 1079 1083 . . 1099 1102 + CviJI RGCY 1100 1100 . . 1105 1109 + Tsp45I GTSAC 1104 1109 . . 1105 1109 + MaeIII GTNAC 1104 1109 . . 1110 1114 - TspDTI ATGAA 1100 1098 . . 1115 1120 + NspBII* CMGCKG 1117 1117 . . 1115 1118 + AciI CCGC 1115 1117 . . 1112 1116 - FokI GGATG 1098 1102 . . 1112 1116 - BseGI GGATG 1111 1109 . . 1117 1123 + EspI* GCTNAGC 1118 1121 . . 1118 1122 + DdeI CTNAG 1118 1121 . . 1121 1124 + CviJI RGCY 1122 1122 . . 1118 1122 - BspCNI CTCAG 1110 1108 . . 1118 1122 - BseMII CTCAG 1109 1107 . . 1129 1133 + Tsp4CI* ACNGT 1131 1130 . . 1134 1140 + TspRI CASTGNN 1140 1131 . . 1141 1145 + Tsp45I GTSAC 1140 1145 . . 1141 1145 + MaeIII GTNAC 1140 1145 . . 1144 1147 + SetI ASST 1147 1143 . . 1145 1148 + MnlI CCTC 1155 1154 . . 1146 1151 + Ksp632I* CTCTTC 1152 1155 . . 1142 1146 - HphI GGTGA 1134 1133 . . 1147 1151 - MboII GAAGA 1139 1138 . . 1158 1161 + SetI ASST 1161 1157 . . 1162 1168 + SexAI ACCWGGT 1162 1167 . . 1163 1167 + BssKI CCNGG 1162 1167 . . 1163 1167 + BseBI CCWGG 1164 1165 . . 1163 1167 + ScrFI CCNGG 1164 1165 . . 1163 1167 + EcoRII CCWGG 1162 1167 . . 1165 1168 + SetI ASST 1168 1164 . . 1169 1172 + MaeI CTAG 1169 1171 . . 1177 1181 + Tsp45I GTSAC 1176 1181 . . 1177 1181 + MaeIII GTNAC 1176 1181 . . 1182 1186 - TspDTI ATGAA 1172 1170 . . 1187 1192 + NspBII* CMGCKG 1189 1189 . . 1187 1190 + AciI CCGC 1187 1189 . . 1184 1188 - FokI GGATG 1170 1174 . . 1184 1188 - BseGI GGATG 1183 1181 . . 1194 1197 + SetI ASST 1197 1193 . . 1201 1205 + Tsp4CI* ACNGT 1203 1202 . . 1206 1212 + TspRI CASTGNN 1212 1203 . . 1210 1215 + EcoRI GAATTC 1210 1214 . . 1210 1215 + ApoI RAATTY 1210 1214 . . 1211 1214 + TspEI AATT 1210 1214 . . 1216 1219 + SetI ASST 1219 1215 . . 1217 1220 + MnlI CCTC 1227 1226 . . 1218 1223 + Ksp632I* CTCTTC 1224 1227 . . 1214 1218 - HphI GGTGA 1206 1205 . . 1219 1223 - MboII GAAGA 1211 1210 . . 1230 1233 + SetI ASST 1233 1229 . . 1234 1240 + SexAI ACCWGGT 1234 1239 . . 1235 1239 + BssKI CCNGG 1234 1239 . . 1235 1239 + BseBI CCWGG 1236 1237 . . 1235 1239 + ScrFI CCNGG 1236 1237 . . 1235 1239 + EcoRII CCWGG 1234 1239 . . 1237 1240 + SetI ASST 1240 1236 . . 1241 1244 + MaeI CTAG 1241 1243 . . 1249 1253 + Tsp45I GTSAC 1248 1253 . . 1249 1253 + MaeIII GTNAC 1248 1253 . . 1254 1258 - TspDTI ATGAA 1244 1242 . . 1259 1264 + NspBII* CMGCKG 1261 1261 . . 1259 1262 + AciI CCGC 1259 1261 . . 1256 1260 - FokI GGATG 1242 1246 . . 1256 1260 - BseGI GGATG 1255 1253 . . 1266 1269 + SetI ASST 1269 1265 . . 1273 1277 + Tsp4CI* ACNGT 1275 1274 . . 1278 1284 + TspRI CASTGNN 1284 1275 . . 1282 1287 + EcoRI GAATTC 1282 1286 . . 1282 1287 + ApoI RAATTY 1282 1286 . . 1283 1286 + TspEI AATT 1282 1286 . . 1288 1291 + SetI ASST 1291 1287 . . 1289 1292 + MnlI CCTC 1299 1298 . . 1290 1295 + Ksp632I* CTCTTC 1296 1299 . . 1286 1290 - HphI GGTGA 1278 1277 . . 1291 1295 - MboII GAAGA 1283 1282 . . 1302 1305 + SetI ASST 1305 1301 . . 1306 1312 + SexAI ACCWGGT 1306 1311 . . 1307 1311 + BssKI CCNGG 1306 1311 . . 1307 1311 + BseBI CCWGG 1308 1309 . . 1307 1311 + ScrFI CCNGG 1308 1309 . . 1307 1311 + EcoRII CCWGG 1306 1311 . . 1309 1312 + SetI ASST 1312 1308 . . 1313 1316 + MaeI CTAG 1313 1315 . . 1321 1325 + Tsp45I GTSAC 1320 1325 . . 1321 1325 + MaeIII GTNAC 1320 1325 . . 1326 1330 - TspDTI ATGAA 1316 1314 . . 1331 1336 + NspBII* CMGCKG 1333 1333 . . 1331 1334 + AciI CCGC 1331 1333 . . 1328 1332 - FokI GGATG 1314 1318 . . 1328 1332 - BseGI GGATG 1327 1325 . . 1338 1341 + SetI ASST 1341 1337 . . 1345 1349 + Tsp4CI* ACNGT 1347 1346 . . 1350 1356 + TspRI CASTGNN 1356 1347 . . 1354 1359 + EcoRI GAATTC 1354 1358 . . 1354 1359 + ApoI RAATTY 1354 1358 . . 1355 1358 + TspEI AATT 1354 1358 . . 1360 1363 + SetI ASST 1363 1359 . . 1361 1364 + MnlI CCTC 1371 1370 . . 1362 1367 + Ksp632I* CTCTTC 1368 1371 . . 1358 1362 - HphI GGTGA 1350 1349 . . 1363 1367 - MboII GAAGA 1355 1354 . . 1374 1377 + SetI ASST 1377 1373 . . 1378 1384 + SexAI ACCWGGT 1378 1383 . . 1379 1383 + BssKI CCNGG 1378 1383 . . 1379 1383 + BseBI CCWGG 1380 1381 . . 1379 1383 + ScrFI CCNGG 1380 1381 . . 1379 1383 + EcoRII CCWGG 1378 1383 . . 1381 1384 + SetI ASST 1384 1380 . . 1385 1388 + MaeI CTAG 1385 1387 . . 1393 1397 + Tsp45I GTSAC 1392 1397 . . 1393 1397 + MaeIII GTNAC 1392 1397 . . 1398 1402 - TspDTI ATGAA 1388 1386 . . 1403 1408 + NspBII* CMGCKG 1405 1405 . . 1403 1406 + AciI CCGC 1403 1405 . . 1400 1404 - FokI GGATG 1386 1390 . . 1400 1404 - BseGI GGATG 1399 1397 . . 1410 1413 + SetI ASST 1413 1409 . . 1417 1421 + Tsp4CI* ACNGT 1419 1418 . . 1422 1428 + TspRI CASTGNN 1428 1419 . . 1426 1431 + EcoRI GAATTC 1426 1430 . . 1426 1431 + ApoI RAATTY 1426 1430 . . 1427 1430 + TspEI AATT 1426 1430 . . 1432 1435 + SetI ASST 1435 1431 . . 1433 1436 + MnlI CCTC 1443 1442 . . 1434 1439 + Ksp632I* CTCTTC 1440 1443 . . 1430 1434 - HphI GGTGA 1422 1421 . . 1435 1439 - MboII GAAGA 1427 1426 . . 1446 1449 + SetI ASST 1449 1445 . . 1450 1456 + SexAI ACCWGGT 1450 1455 . . 1451 1455 + BssKI CCNGG 1450 1455 . . 1451 1455 + BseBI CCWGG 1452 1453 . . 1451 1455 + ScrFI CCNGG 1452 1453 . . 1451 1455 + EcoRII CCWGG 1450 1455 . . 1453 1456 + SetI ASST 1456 1452 . . 1457 1460 + MaeI CTAG 1457 1459 . . 1465 1469 + Tsp45I GTSAC 1464 1469 . . 1465 1469 + MaeIII GTNAC 1464 1469 . . 1470 1474 - TspDTI ATGAA 1460 1458 . . 1475 1480 + NspBII* CMGCKG 1477 1477 . . 1475 1478 + AciI CCGC 1475 1477 . . 1472 1476 - FokI GGATG 1458 1462 . . 1472 1476 - BseGI GGATG 1471 1469 . . 1482 1485 + SetI ASST 1485 1481 . . 1489 1493 + Tsp4CI* ACNGT 1491 1490 . . 1494 1500 + TspRI CASTGNN 1500 1491 . . 1498 1503 + EcoRI GAATTC 1498 1502 . . 1498 1503 + ApoI RAATTY 1498 1502 . . 1499 1502 + TspEI AATT 1498 1502 . . 1504 1507 + SetI ASST 1507 1503 . . 1505 1508 + MnlI CCTC 1515 1514 . . 1506 1511 + Ksp632I* CTCTTC 1512 1515 . . 1502 1506 - HphI GGTGA 1494 1493 . . 1507 1511 - MboII GAAGA 1499 1498 . . 1518 1521 + SetI ASST 1521 1517 . . 1525 1528 + SetI ASST 1528 1524 . . 1529 1532 + MaeI CTAG 1529 1531 . . 1537 1541 + Tsp45I GTSAC 1536 1541 . . 1537 1541 + MaeIII GTNAC 1536 1541 . . 1542 1546 - TspDTI ATGAA 1532 1530 . . 1547 1552 + NspBII* CMGCKG 1549 1549 . . 1547 1550 + AciI CCGC 1547 1549 . . 1544 1548 - FokI GGATG 1530 1534 . . 1544 1548 - BseGI GGATG 1543 1541 . . 1554 1557 + SetI ASST 1557 1553 . . 1561 1565 + Tsp4CI* ACNGT 1563 1562 . . 1573 1577 + Tsp45I GTSAC 1572 1577 . . 1573 1577 + MaeIII GTNAC 1572 1577 . . 1576 1579 + SetI ASST 1579 1575 . . 1577 1580 + MnlI CCTC 1587 1586 . . 1578 1583 + Ksp632I* CTCTTC 1584 1587 . . 1574 1578 - HphI GGTGA 1566 1565 . . 1579 1583 - MboII GAAGA 1571 1570 . . 1590 1593 + SetI ASST 1593 1589 . . 1597 1600 + SetI ASST 1600 1596 . . 1601 1604 + MaeI CTAG 1601 1603 . . 1609 1613 + Tsp45I GTSAC 1608 1613 . . 1609 1613 + MaeIII GTNAC 1608 1613 . . 1619 1627 + AlwNI CAGNNNCTG 1624 1621 . . 1623 1626 + SetI ASST 1626 1622 . . 1630 1634 - MboII GAAGA 1622 1621 . . 1634 1638 + Hin4II* CCTTC 1644 1643 . . 1641 1646 + Hpy166II GTNNAC 1643 1643 . . 1641 1646 + AccI GTMKAC 1642 1644 . . 1650 1656 + TspRI CASTGNN 1656 1647 . . 1660 1663 + SetI ASST 1663 1659 . . 1661 1664 + MnlI CCTC 1671 1670 . . 1662 1667 + Ksp632I* CTCTTC 1668 1671 . . 1658 1662 - HphI GGTGA 1650 1649 . . 1663 1667 - MboII GAAGA 1655 1654 . . 1667 1672 - EcoP15I CAGCAG 1639 1641 . . 1674 1677 + SetI ASST 1677 1673 . . 1675 1678 + MnlI CCTC 1685 1684 . . 1703 1706 + AciI CCGC 1703 1705 . . 1708 1712 + TfiI GAWTC 1708 1711 . . 1708 1712 + HinfI GANTC 1708 1711 . . 1718 1721 + MnlI CCTC 1728 1727 . . 1716 1721 - BseRI GAGGAG 1707 1705 . . 1730 1734 - HphI GGTGA 1722 1721 . . 1740 1746 + TspRI CASTGNN 1746 1737 . . 1742 1747 + Hpy166II GTNNAC 1744 1744 . . 1739 1743 - BsrI ACTGG 1739 1737 . . 1757 1760 + MaeI CTAG 1757 1759 . . 1764 1767 + TspEI AATT 1763 1767 . . 1772 1775 + MnlI CCTC 1782 1781 . . 1773 1778 + Ksp632I* CTCTTC 1779 1782 . . 1777 1781 + Hpy188I TCNGA 1779 1778 . . 1774 1778 - MboII GAAGA 1766 1765 . . 1778 1782 + DdeI CTNAG 1778 1781 . . 1782 1786 + TfiI GAWTC 1782 1785 . . 1782 1786 + HinfI GANTC 1782 1785 . . 1778 1782 - BspCNI CTCAG 1770 1768 . . 1778 1782 - BseMII CTCAG 1769 1767 . . 1786 1790 + Hin4II* CCTTC 1796 1795 . . 1789 1793 - MboII GAAGA 1781 1780 . . 1800 1805 + TatI WGTACW 1800 1804 . . 1800 1805 + ScaI AGTACT 1802 1802 . . 1801 1804 + Csp6I GTAC 1801 1803 . . 1801 1804 + RsaI GTAC 1802 1802 . . 1817 1821 + BinI* GGATC 1825 1826 . . 1814 1817 - AciI CCGC 1814 1816 . . 1814 1818 - FauI CCCGC 1807 1809 . . 1818 1821 + DpnI GATC 1819 1819 . . 1818 1821 + BstKTI GATC 1820 1818 . . 1818 1821 + MboI GATC 1817 1821 . . 1824 1828 - TspDTI ATGAA 1814 1812 . . 1838 1849 + BstXI CCANNNNNNTGG 1845 1841 . . 1852 1856 + BmgT120I GGNCC 1853 1854 . . 1852 1856 + AvaII GGWCC 1852 1855 . . 1852 1856 + AsuI* GGNCC 1852 1855 . . 1854 1857 + SetI ASST 1857 1853 . . 1849 1854 - MmeI TCCRAC 1830 1828 . . 1855 1859 + Hin4II* CCTTC 1865 1864 . . 1861 1865 + Hpy188I TCNGA 1863 1862 . . 1858 1862 - MboII GAAGA 1850 1849 . . 1865 1869 + TspDTI ATGAA 1880 1878 . . 1867 1871 + TfiI GAWTC 1867 1870 . . 1867 1871 + HinfI GANTC 1867 1870 . . 1876 1880 - MboII GAAGA 1868 1867 . . 1901 1905 - FokI GGATG 1887 1891 . . 1901 1905 - BseGI GGATG 1900 1898 . . 1917 1920 + CviJI RGCY 1918 1918 . . 1913 1918 - Eco57MI CTGRAG 1898 1896 . . 1913 1918 - Eco57I CTGAAG 1898 1896 . . 1918 1922 - BceAI ACGGC 1903 1905 . . 1928 1935 + PspXI VCTCGAGB 1929 1933 . . 1928 1931 + MnlI CCTC 1938 1937 . . 1929 1934 + SmlI CTYRAG 1929 1933 . . 1929 1934 + XhoI CTCGAG 1929 1933 . . 1929 1934 + BmeT110I CYCGRG 1930 1932 . . 1929 1934 + AvaI CYCGRG 1929 1933 . . 1930 1933 + TaqI TCGA 1930 1932 . . 1938 1941 + CviJI RGCY 1939 1939 . . 1939 1943 + BlsI GCNGC 1941 1940 . . 1939 1943 + BisI GCNGC 1940 1941 . . 1939 1943 + TseI GCWGC 1939 1942 . . 1939 1943 - BbvI GCAGC 1926 1930 . . 1940 1945 - EcoP15I CAGCAG 1912 1914 . . 1957 1962 + XhoII RGATCY 1957 1961 . . 1958 1961 + DpnI GATC 1959 1959 . . 1958 1961 + BstKTI GATC 1960 1958 . . 1958 1961 + MboI GATC 1957 1961 . . 1962 1968 + TspRI CASTGNN 1968 1959 . . 1958 1962 - BinI* GGATC 1952 1953 . . 1963 1967 + Tsp4CI* ACNGT 1965 1964 . . 1978 1982 + Hpy188I TCNGA 1980 1979 . . 1984 1988 + BsmAI GTCTC 1989 1993 . . 1991 1995 + MaeIII GTNAC 1990 1995 . . 1997 2000 + MnlI CCTC 2007 2006 . . 1995 2000 - BseRI GAGGAG 1986 1984 . . 2014 2017 + SetI ASST 2017 2013 . . 2029 2033 + Hin4II* CCTTC 2039 2038 . . 2033 2044 - CspCI CAANNNNNGTGG 2057 2055 2022 2020 2050 2054 - MboII GAAGA 2042 2041 . . 2056 2060 + Tsp4CI* ACNGT 2058 2057 . . 2062 2066 + Tsp45I GTSAC 2061 2066 . . 2062 2066 + MaeIII GTNAC 2061 2066 . . 2066 2069 + MaeI CTAG 2066 2068 . . 2068 2071 + SetI ASST 2071 2067 . . 2068 2071 + CviJI RGCY 2069 2069 . . 2068 2071 + AluI AGCT 2069 2069 . . 2077 2081 + TfiI GAWTC 2077 2080 . . 2077 2081 + HinfI GANTC 2077 2080 . . 2092 2097 + SspI AATATT 2094 2094 . . 2099 2103 + Hin4II* CCTTC 2109 2108 . . 2103 2109 + TspRI CASTGNN 2109 2100 . . 2100 2105 - Eco57MI CTGRAG 2085 2083 . . 2100 2105 - Eco57I CTGAAG 2085 2083 . . 2117 2120 + MnlI CCTC 2127 2126 . . 2121 2126 + Hpy178III* TCNNGA 2122 2124 . . 2125 2129 + PleI GAGTC 2133 2134 . . 2125 2129 + MlyI GAGTC 2134 2134 . . 2125 2129 + HinfI GANTC 2125 2128 . . 2120 2125 - Eco57MI CTGRAG 2105 2103 . . 2120 2125 - GsuI CTGGAG 2105 2103 . . 2130 2135 + Hpy166II GTNNAC 2132 2132 . . 2130 2135 + AccI GTMKAC 2131 2133 . . 2136 2139 + CviRI* TGCA 2137 2137 . . 2139 2142 + TspEI AATT 2138 2142 . . 2143 2146 + TaiI ACGT 2146 2142 . . 2143 2146 + SetI ASST 2146 2142 . . 2143 2146 + MaeII ACGT 2143 2145 . . 2145 2150 + Hpy166II GTNNAC 2147 2147 . . 2145 2150 + AccI GTMKAC 2146 2148 . . 2150 2154 - FokI GGATG 2136 2140 . . 2150 2154 - BseGI GGATG 2149 2147 . . 2156 2159 + MaeI CTAG 2156 2158 . . 2158 2161 + SetI ASST 2161 2157 . . 2158 2161 + CviJI RGCY 2159 2159 . . 2158 2161 + AluI AGCT 2159 2159 . . 2161 2165 - TspDTI ATGAA 2151 2149 . . 2167 2173 + EspI* GCTNAGC 2168 2171 . . 2168 2172 + DdeI CTNAG 2168 2171 . . 2163 2168 - BsrDI GCAATG 2162 2160 . . 2168 2172 - BspCNI CTCAG 2160 2158 . . 2168 2172 - BseMII CTCAG 2159 2157 . . 2172 2175 - AciI CCGC 2172 2174 . . 2170 2175 - BsrBI CCGCTC 2172 2172 . . 2179 2183 - MboII GAAGA 2171 2170 . . 2211 2221 + FalI AAGNNNNNCTT 2202 2197 2234 2229 2221 2224 + MseI TTAA 2221 2223 . . 2211 2221 - FalI AAGNNNNNCTT 2234 2229 2202 2197 2224 2229 + TatI WGTACW 2224 2228 . . 2224 2229 + ScaI AGTACT 2226 2226 . . 2225 2228 + Csp6I GTAC 2225 2227 . . 2225 2228 + RsaI GTAC 2226 2226 . . 2232 2238 + TspRI CASTGNN 2238 2229 . . 2233 2237 + Tsp4CI* ACNGT 2235 2234 . . 2244 2249 + ClaI ATCGAT 2245 2247 . . 2245 2248 + TaqI TCGA 2245 2247 . . 2246 2251 + McrI* CGRYCG 2249 2247 . . 2246 2251 + PvuI CGATCG 2249 2247 . . 2247 2250 + DpnI GATC 2248 2248 . . 2247 2250 + BstKTI GATC 2249 2247 . . 2247 2250 + MboI GATC 2246 2250 . . 2250 2255 + Esp3I CGTCTC 2256 2260 . . 2251 2255 + BsmAI GTCTC 2256 2260 . . 2253 2258 + Ksp632I* CTCTTC 2259 2262 . . 2254 2258 - MboII GAAGA 2246 2245 . . 2256 2260 - TspDTI ATGAA 2246 2244 . . 2268 2273 + NlaIV GGNNCC 2270 2270 . . 2271 2274 + SetI ASST 2274 2270 . . 2287 2291 + SfaNI GCATC 2296 2300 . . 2287 2297 + MwoI GCNNNNNNNGC 2293 2290 . . 2287 2297 + BstAPI GCANNNNNTGC 2293 2290 . . 2295 2298 + CviRI* TGCA 2296 2296 . . 2296 2300 + SfaNI GCATC 2305 2309 . . 2308 2311 + MnlI CCTC 2318 2317 . . 2313 2317 + DdeI CTNAG 2313 2316 . . 2317 2322 + BciVI GTATCC 2328 2327 . . 2321 2324 + MnlI CCTC 2331 2330 . . 2322 2327 + SmlI CTYRAG 2322 2326 . . 2323 2328 + Hpy178III* TCNNGA 2324 2326 . . 2322 2327 - Bce83I* CTTGAG 2307 2305 . . 2332 2335 + TspEI AATT 2331 2335 . . 2335 2338 + CviRI* TGCA 2336 2336 . . 2336 2341 + BtsI GCAGTG 2343 2341 . . 2337 2343 + TspRI CASTGNN 2343 2334 . . 2341 2345 - SfaNI GCATC 2331 2335 . . 2353 2358 + MmeI TCCRAC 2378 2376 . . 2360 2364 + BccI CCATC 2368 2369 . . 2369 2372 - AciI CCGC 2369 2371 . . 2389 2398 + XmnI GAANNNNTTC 2393 2393 . . 2403 2406 + CviRI* TGCA 2404 2404 . . 2407 2410 + SetI ASST 2410 2406 . . 2408 2411 + MnlI CCTC 2418 2417 . . 2423 2426 + SetI ASST 2426 2422 . . 2435 2439 + TspDTI ATGAA 2450 2448 . . 2439 2442 + TspEI AATT 2438 2442 . . 2447 2452 + Hpy178III* TCNNGA 2448 2450 . . 2448 2453 + BsiI* CACGAG 2448 2452 . . 2452 2455 + SetI ASST 2455 2451 . . 2452 2455 + CviJI RGCY 2453 2453 . . 2452 2455 + AluI AGCT 2453 2453 . . 2467 2472 + TatI WGTACW 2467 2471 . . 2468 2471 + Csp6I GTAC 2468 2470 . . 2468 2471 + RsaI GTAC 2469 2469 . . 2474 2478 + TspDTI ATGAA 2489 2487 . . 2476 2480 + MboII GAAGA 2488 2487 . . 2490 2500 + Hin4I GAYNNNNNVTC 2481 2476 2513 2508 2498 2503 + SmlI CTYRAG 2498 2502 . . 2498 2503 - Bce83I* CTTGAG 2483 2481 . . 2509 2513 + Tsp4CI* ACNGT 2511 2510 . . 2527 2532 + SpeI ACTAGT 2527 2531 . . 2528 2531 + MaeI CTAG 2528 2530 . . 2549 2552 + Csp6I GTAC 2549 2551 . . 2549 2552 + RsaI GTAC 2550 2550 . . 2553 2562 + MslI CAYNNNNRTG 2557 2557 . . 2562 2566 + BsmAI GTCTC 2567 2571 . . 2565 2570 + BetI* WCCGGW 2565 2569 . . 2566 2569 + HpaII CCGG 2566 2568 . . 2569 2573 + MaeIII GTNAC 2568 2573 . . 2578 2583 + VspI ATTAAT 2579 2581 . . 2579 2582 + MseI TTAA 2579 2581 . . 2585 2589 + DdeI CTNAG 2585 2588 . . 2584 2590 - Bpu10I CCTNAGC 2585 2588 . . 2599 2602 + MseI TTAA 2599 2601 . . 2606 2611 + SfeI* CTRYAG 2606 2610 . . 2618 2623 + DsaI* CCRYGG 2618 2622 . . 2618 2623 + SecI* CCNNGG 2618 2622 . . 2620 2624 + Tsp4CI* ACNGT 2622 2621 . . 2621 2626 + McrI* CGRYCG 2624 2622 . . 2624 2627 + TaqI TCGA 2624 2626 . . 2641 2646 + NspI RCATGY 2645 2641 . . 2642 2645 + FatI CATG 2641 2645 . . 2642 2645 + NlaIII CATG 2645 2641 . . 2642 2645 + CviAII CATG 2642 2644 . . 2646 2651 + SecI* CCNNGG 2646 2650 . . 2647 2651 + BssKI CCNGG 2646 2651 . . 2647 2651 + BseBI CCWGG 2648 2649 . . 2647 2651 + ScrFI CCNGG 2648 2649 . . 2647 2651 + EcoRII CCWGG 2646 2651 . . 2662 2665 + TaqI TCGA 2662 2664 . . 2671 2675 + Tsp4CI* ACNGT 2673 2672 . . 2680 2684 + MaeIII GTNAC 2679 2684 . . 2677 2681 - BsrI ACTGG 2677 2675 . . 2690 2694 - HphI GGTGA 2682 2681 . . 2700 2703 + CviJI RGCY 2701 2701 . . 2711 2714 + AciI CCGC 2711 2713 . . 2728 2732 + MboII GAAGA 2740 2739 . . 2735 2739 + TspDTI ATGAA 2750 2748 . . 2737 2741 + MboII GAAGA 2749 2748 . . 2740 2744 + MboII GAAGA 2752 2751 . . 2743 2748 + EcoRV GATATC 2745 2745 . . 2791 2796 + SpeI ACTAGT 2791 2795 . . 2792 2795 + MaeI CTAG 2792 2794 . . 2813 2816 + Csp6I GTAC 2813 2815 . . 2813 2816 + RsaI GTAC 2814 2814 . . 2817 2826 + MslI CAYNNNNRTG 2821 2821 . . 2826 2830 + BsmAI GTCTC 2831 2835 . . 2829 2834 + BetI* WCCGGW 2829 2833 . . 2830 2833 + HpaII CCGG 2830 2832 . . 2833 2837 + MaeIII GTNAC 2832 2837 . . 2842 2847 + VspI ATTAAT 2843 2845 . . 2843 2846 + MseI TTAA 2843 2845 . . 2849 2853 + DdeI CTNAG 2849 2852 . . 2848 2854 - Bpu10I CCTNAGC 2849 2852 . . 2863 2866 + MseI TTAA 2863 2865 . . 2870 2875 + SfeI* CTRYAG 2870 2874 . . 2882 2887 + DsaI* CCRYGG 2882 2886 . . 2882 2887 + SecI* CCNNGG 2882 2886 . . 2884 2888 + Tsp4CI* ACNGT 2886 2885 . . 2885 2890 + McrI* CGRYCG 2888 2886 . . 2888 2891 + TaqI TCGA 2888 2890 . . 2905 2910 + NspI RCATGY 2909 2905 . . 2906 2909 + FatI CATG 2905 2909 . . 2906 2909 + NlaIII CATG 2909 2905 . . 2906 2909 + CviAII CATG 2906 2908 . . 2926 2929 + TaqI TCGA 2926 2928 . . 2935 2939 + Tsp4CI* ACNGT 2937 2936 . . 2944 2948 + MaeIII GTNAC 2943 2948 . . 2941 2945 - BsrI ACTGG 2941 2939 . . 2954 2958 - HphI GGTGA 2946 2945 . . 2975 2978 + AciI CCGC 2975 2977 . . 2992 2996 + MboII GAAGA 3004 3003 . . 2999 3003 + TspDTI ATGAA 3014 3012 . . 3001 3005 + MboII GAAGA 3013 3012 . . 3004 3008 + MboII GAAGA 3016 3015 . . 3007 3017 + Hin4I GAYNNNNNVTC 2998 2993 3030 3025 3014 3017 + MnlI CCTC 3024 3023 . . 3055 3060 + SpeI ACTAGT 3055 3059 . . 3056 3059 + MaeI CTAG 3056 3058 . . 3076 3079 + CviRI* TGCA 3077 3077 . . 3081 3090 + MslI CAYNNNNRTG 3085 3085 . . 3090 3094 + BsmAI GTCTC 3095 3099 . . 3093 3098 + BetI* WCCGGW 3093 3097 . . 3094 3097 + HpaII CCGG 3094 3096 . . 3117 3120 + CviJI RGCY 3118 3118 . . 3127 3130 + TaqI TCGA 3127 3129 . . 3135 3138 + CviRI* TGCA 3136 3136 . . 3139 3143 + PleI GAGTC 3147 3148 . . 3139 3143 + MlyI GAGTC 3148 3148 . . 3139 3143 + HinfI GANTC 3139 3142 . . 3141 3146 + SalI GTCGAC 3141 3145 . . 3141 3146 + Hpy166II GTNNAC 3143 3143 . . 3141 3146 + HindII GTYRAC 3143 3143 . . 3141 3146 + AccI GTMKAC 3142 3144 . . 3142 3145 + TaqI TCGA 3142 3144 . . 3146 3150 + BccI CCATC 3154 3155 . . 3147 3151 - FokI GGATG 3133 3137 . . 3147 3151 - BseGI GGATG 3146 3144 . . 3161 3164 + MseI TTAA 3161 3163 . . 3181 3186 + NlaIV GGNNCC 3183 3183 . . 3178 3181 - AciI CCGC 3178 3180 . . 3181 3186 + HgiCI* GGYRCC 3181 3185 . . 3178 3182 - FauI CCCGC 3171 3173 . . 3188 3191 + Csp6I GTAC 3188 3190 . . 3188 3191 + RsaI GTAC 3189 3189 . . 3198 3207 + PshAI GACNNNNGTC 3202 3202 . . 3202 3206 + Tsp4CI* ACNGT 3204 3203 . . 3206 3209 + TaqI TCGA 3206 3208 . . 3210 3213 + CviRI* TGCA 3211 3211 . . 3208 3212 - SfaNI GCATC 3198 3202 . . 3221 3226 + NspI RCATGY 3225 3221 . . 3222 3225 + FatI CATG 3221 3225 . . 3222 3225 + NlaIII CATG 3225 3221 . . 3222 3225 + CviAII CATG 3222 3224 . . 3225 3230 + Hpy166II GTNNAC 3227 3227 . . 3235 3240 + BetI* WCCGGW 3235 3239 . . 3236 3239 + HpaII CCGG 3236 3238 . . 3239 3249 + Hin4I GAYNNNNNVTC 3230 3225 3262 3257 3239 3249 - Hin4I GAYNNNNNVTC 3262 3257 3230 3225 3259 3263 + Tsp4CI* ACNGT 3261 3260 . . 3262 3266 + Tsp45I GTSAC 3261 3266 . . 3262 3266 + MaeIII GTNAC 3261 3266 . . 3273 3277 + DdeI CTNAG 3273 3276 . . 3273 3277 + BspCNI CTCAG 3286 3284 . . 3273 3277 + BseMII CTCAG 3287 3285 . . 3276 3279 + SetI ASST 3279 3275 . . 3272 3278 - Bpu10I CCTNAGC 3273 3276 . . 3280 3285 + NlaIV GGNNCC 3282 3282 . . 3283 3286 + SetI ASST 3286 3282 . . 3287 3290 + Csp6I GTAC 3287 3289 . . 3287 3290 + RsaI GTAC 3288 3288 . . 3291 3294 + FatI CATG 3290 3294 . . 3291 3294 + NlaIII CATG 3294 3290 . . 3291 3294 + CviAII CATG 3291 3293 . . 3294 3299 + Hpy166II GTNNAC 3296 3296 . . 3294 3299 + HpaI GTTAAC 3296 3296 . . 3294 3299 + HindII GTYRAC 3296 3296 . . 3295 3298 + MseI TTAA 3295 3297 . . 3300 3303 + CviRI* TGCA 3301 3301 . . 3301 3311 + MwoI GCNNNNNNNGC 3307 3304 . . 3299 3304 - BsgI GTGCAG 3284 3282 . . 3308 3313 + Hpy178III* TCNNGA 3309 3311 . . 3308 3313 + NruI TCGCGA 3310 3310 . . 3309 3312 + FnuDII* CGCG 3310 3310 . . 3311 3316 + McrI* CGRYCG 3314 3312 . . 3314 3317 + AciI CCGC 3314 3316 . . 3334 3338 - HphI GGTGA 3326 3325 . . 3339 3342 + TspEI AATT 3338 3342 . . 3348 3351 + CviJI RGCY 3349 3349 . . 3349 3359 + MwoI GCNNNNNNNGC 3355 3352 . . 3349 3359 + BglI GCCNNNNNGGC 3355 3352 . . 3350 3353 + MnlI CCTC 3360 3359 . . 3353 3356 + MnlI CCTC 3363 3362 . . 3353 3358 + SecI* CCNNGG 3353 3357 . . 3351 3356 - BseRI GAGGAG 3342 3340 . . 3357 3360 + CviJI RGCY 3358 3358 . . 3354 3359 - NmeAIII GCCGAG 3334 3332 . . 3366 3371 - BsrDI GCAATG 3365 3363 . . 3375 3379 - TspDTI ATGAA 3365 3363 . . 3391 3395 + Tsp4CI* ACNGT 3393 3392 . . 3410 3414 + Tsp4CI* ACNGT 3412 3411 . . 3412 3417 + NlaIV GGNNCC 3414 3414 . . 3412 3417 + HgiCI* GGYRCC 3412 3416 . . 3421 3424 + TspEI AATT 3420 3424 . . 3425 3428 + TaqI TCGA 3425 3427 . . 3445 3450 + KpnI GGTACC 3449 3445 . . 3445 3450 + NlaIV GGNNCC 3447 3447 . . 3445 3450 + HgiCI* GGYRCC 3445 3449 . . 3445 3450 + Acc65I GGTACC 3445 3449 . . 3446 3449 + Csp6I GTAC 3446 3448 . . 3446 3449 + RsaI GTAC 3447 3447 . . 3449 3452 + AciI CCGC 3449 3451 . . 3451 3461 + MwoI GCNNNNNNNGC 3457 3454 . . 3460 3470 + MwoI GCNNNNNNNGC 3466 3463 . . 3481 3486 + SfeI* CTRYAG 3481 3485 . . #--------------------------------------- # # # AarI: # [CACCTGC] # 987 2499 # # Acc65I: # [GGTACC] # 41 3445 # # AccI: # [GTMKAC] # 15 344 489 996 1642 # # AciI: # [CCGC] # 37 49 54 72 72 72 # 72 72 72 111 135 136 # 156 197 203 249 264 342 # 358 763 # # AgeI: # [ACCGGT] # 247 3239 # # AjuI: # [GAANNNNNNNTTGG] # 32 357 3097 # # AluI: # [AGCT] # 40 90 294 1033 2029 # # AlwNI: # [CAGNNNCTG] # 1624 1862 # # ApoI: # [RAATTY] # 72 72 72 72 1210 1988 # # AsuI*: # [GGNCC] # 1634 1852 # # AvaI: # [CYCGRG] # 1557 1929 # # AvaII: # [GGWCC] # 1634 1852 # # BbvI: # [GCAGC] # 3 39 178 1560 1706 # # BccI: # [CCATC] # 332 337 786 2031 # # Bce83I*: # [CTTGAG] # 176 1003 2307 # # BceAI: # [ACGGC] # 175 196 636 896 1583 # # BciVI: # [GTATCC] # 1158 2328 # # BclI: # [TGATCA] # 278 3208 # # BetI*: # [WCCGGW] # 83 142 164 251 264 264 # 2318 # # BglI: # [GCCNNNNNGGC] # 131 3355 # # BinI*: # [GGATC] # 127 1534 1825 # # BisI: # [GCNGC] # 3 3 50 153 1546 1731 # # BlsI: # [GCNGC] # 3 3 51 153 1545 1731 # # BmeT110I: # [CYCGRG] # 1556 1930 # # BmgT120I: # [GGNCC] # 1633 1853 # # Bpu10I: # [CCTNAGC] # 36 213 264 424 2549 # # BsaXI: # [ACNNNNNCTCC] # 30 63 3393 # # BseBI: # [CCWGG] # 72 72 72 72 838 1164 # 1196 # # BseGI: # [GGATG] # 72 72 72 72 72 72 # 192 249 340 348 357 571 # 997 # # BseMII: # [CTCAG] # 199 390 660 1109 1128 # # BseRI: # [GAGGAG] # 144 279 1356 1707 # # BseYI: # [CCCAGC] # 1096 2390 # # BsgI: # [GTGCAG] # 202 3284 # # BsiI*: # [CACGAG] # 1038 2448 # # BslFI: # [GGGAC] # 1079 2407 # # BsmAI: # [GTCTC] # 264 264 267 311 391 963 # 1026 # # BsmI: # [GAATGC] # 1036 2450 # # BspCNI: # [CTCAG] # 200 390 660 1110 1126 # # BspMI: # [ACCTGC] # 987 2499 # # BspMII*: # [TCCGGA] # 164 3322 # # BsrBI: # [CCGCTC] # 54 814 1304 1314 # # BsrDI: # [GCAATG] # 121 1203 2162 # # BsrI: # [ACTGG] # 264 288 545 677 774 938 # # BssKI: # [CCNGG] # 72 72 72 72 840 1162 # 1196 # # BstAPI: # [GCANNNNNTGC] # 1193 2293 # # BstKTI: # [GATC] # 140 281 289 1237 1539 # # BstXI: # [CCANNNNNNTGG] # 1641 1845 # # BtsI: # [GCAGTG] # 938 1143 1405 # # Cac8I: # [GCNNGC] # 317 3169 # # Cfr10I: # [RCCGGY] # 68 247 3171 # # ClaI: # [ATCGAT] # 1241 2245 # # Csp6I: # [GTAC] # 40 81 99 101 144 153 # 159 243 264 375 424 504 # 899 # # CspCI: # [CAANNNNNGTGG] # 35 1429 2022 # # CviAII: # [CATG] # 69 195 264 316 2642 # # CviJI: # [RGCY] # 4 6 9 12 21 22 # 24 34 39 63 72 90 # 104 106 128 130 231 248 # 294 417 636 796 # # CviRI*: # [TGCA] # 40 59 68 75 90 132 # 159 185 673 919 1086 # # DdeI: # [CTNAG] # 36 145 213 264 272 390 # 424 660 1082 # # DpnI: # [GATC] # 140 280 289 1238 1539 # # DsaI*: # [CCRYGG] # 264 292 604 2326 # # Eco47III: # [AGCGCT] # 332 3154 # # Eco57I: # [CTGAAG] # 187 311 1401 1587 # # Eco57MI: # [CTGRAG] # 20 187 311 1381 1587 # # EcoP15I: # [CAGCAG] # 236 273 1403 1574 # # EcoRI: # [GAATTC] # 72 72 72 72 1210 1988 # # EcoRII: # [CCWGG] # 72 72 72 72 840 1162 # 1196 # # EcoRV: # [GATATC] # 741 2745 # # Esp3I: # [CGTCTC] # 1230 2256 # # EspI*: # [GCTNAGC] # 1050 1118 1318 # # FalI: # [AAGNNNNNCTT] # 0 0 32 1252 2202 # # FatI: # [CATG] # 69 196 264 316 2641 # # FauI: # [CCCGC] # 315 1364 1807 # # FnuDII*: # [CGCG] # 176 3310 # # FokI: # [GGATG] # 72 72 72 72 72 72 # 192 249 353 355 357 551 # 997 # # GlaI: # [GCGC] # 332 3154 # # GsaI: # [CCCAGC] # 1100 2386 # # GsuI: # [CTGGAG] # 1381 2105 # # HaeII: # [RGCGCY] # 334 3152 # # HgiCI*: # [GGYRCC] # 33 41 231 3181 # # HgiJII*: # [GRGCYC] # 141 3345 # # HhaI: # [GCGC] # 333 3153 # # Hin4I: # [GAYNNNNNVTC] # 0 0 32 32 32 200 # 224 485 2481 # # Hin4II*: # [CCTTC] # 69 70 152 174 203 1377 # 1441 # # Hin6I: # [GCGC] # 331 3155 # # HindII: # [GTYRAC] # 99 153 190 3044 # # HinfI: # [GANTC] # 48 73 74 85 95 210 # 347 1014 1540 # # HpaI: # [GTTAAC] # 190 3296 # # HpaII: # [CCGG] # 68 83 142 165 250 264 # 264 2250 # # HphI: # [GGTGA] # 33 72 72 72 72 72 # 72 72 75 84 160 163 # 264 380 863 960 # # Hpy166II: # [GTNNAC] # 15 69 84 99 101 190 # 388 996 1544 # # Hpy178III*: # [TCNNGA] # 124 165 177 202 861 1957 # # Hpy188I: # [TCNGA] # 7 62 84 117 129 1506 # 1581 # # KpnI: # [GGTACC] # 37 3449 # # Ksp632I*: # [CTCTTC] # 72 72 72 72 72 72 # 84 111 330 480 822 1227 # # MaeI: # [CTAG] # 44 72 72 72 72 72 # 72 90 108 123 153 156 # 228 264 264 309 372 430 # 513 # # MaeII: # [ACGT] # 1343 2143 # # MaeIII: # [GTNAC] # 36 36 36 36 71 72 # 72 72 72 72 111 111 # 153 225 267 318 382 507 # 837 # # MboI: # [GATC] # 140 278 289 1240 1539 # # MboII: # [GAAGA] # 3 3 9 9 15 18 # 33 51 69 72 72 72 # 72 72 72 75 111 129 # 174 242 252 252 317 470 # 822 # # McrI*: # [CGRYCG] # 172 264 375 426 2249 # # MlyI: # [GAGTC] # 104 338 1014 2030 # # MmeI: # [TCCRAC] # 548 1108 1830 # # MnlI: # [CCTC] # 3 9 13 14 27 36 # 36 36 43 54 54 54 # 54 63 69 72 72 72 # 72 72 72 81 84 87 # 96 99 120 123 132 156 # 191 336 378 606 # # MroNI: # [GCCGGC] # 315 3171 # # MseI: # [TTAA] # 5 20 20 134 191 244 # 298 305 358 1911 # # MslI: # [CAYNNNNRTG] # 264 264 401 2557 # # MwoI: # [GCNNNNNNNGC] # 9 15 20 46 48 102 # 1014 2232 # # NaeI: # [GCCGGC] # 317 3169 # # NlaIII: # [CATG] # 69 192 264 316 2645 # # NlaIV: # [GGNNCC] # 33 39 99 132 913 2270 # # NmeAIII: # [GCCGAG] # 152 3334 # # NruI: # [TCGCGA] # 176 3310 # # NspBII*: # [CMGCKG] # 72 72 72 72 72 72 # 1117 1937 # # NspI: # [RCATGY] # 261 264 316 2645 # # PleI: # [GAGTC] # 103 339 1014 2030 # # PshAI: # [GACNNNNGTC] # 284 3202 # # PspXI: # [VCTCGAGB] # 1557 1929 # # PvuI: # [CGATCG] # 1237 2249 # # RsaI: # [GTAC] # 39 81 99 101 144 154 # 159 243 264 375 424 504 # 899 # # SalI: # [GTCGAC] # 345 3141 # # ScaI: # [AGTACT] # 154 245 424 504 899 1260 # # ScrFI: # [CCNGG] # 72 72 72 72 838 1164 # 1196 # # SduI: # [GDGCHC] # 141 3345 # # SecI*: # [CCNNGG] # 28 133 236 292 471 2326 # # SetI: # [ASST] # 7 7 7 7 7 7 # 7 7 14 14 14 14 # 14 14 14 14 15 16 # 22 22 22 22 22 22 # 26 29 29 29 29 29 # 29 29 29 34 36 36 # 37 42 54 54 54 54 # 62 72 75 81 96 99 # 113 132 136 160 180 200 # 266 824 # # SexAI: # [ACCWGGT] # 72 72 72 72 1162 2036 # # SfaNI: # [GCATC] # 9 23 26 288 867 905 # 1368 # # SfeI*: # [CTRYAG] # 5 264 611 2606 # # SmlI: # [CTYRAG] # 176 393 988 1929 # # SpeI: # [ACTAGT] # 43 264 264 431 2484 # # SspI: # [AATATT] # 1392 2094 # # TaiI: # [ACGT] # 1340 2146 # # TaqI: # [TCGA] # 15 27 38 38 45 54 # 54 54 61 64 126 163 # 201 219 226 240 315 379 # 1167 # # TatI: # [WGTACW] # 152 243 245 424 504 899 # 1019 # # TauI: # [GCSGC] # 52 3434 # # TfiI: # [GAWTC] # 74 85 168 210 1409 1540 # # TseI: # [GCWGC] # 3 52 153 1547 1731 # # Tsp45I: # [GTSAC] # 36 36 36 36 72 72 # 72 72 72 225 453 1104 # 1200 # # Tsp4CI*: # [ACNGT] # 19 51 51 57 72 72 # 72 72 72 72 74 93 # 111 132 177 213 226 267 # 276 402 905 # # TspDTI: # [ATGAA] # 39 66 72 72 72 72 # 72 72 95 104 121 174 # 204 261 264 271 282 351 # 822 # # TspEI: # [AATT] # 26 66 72 72 72 72 # 82 83 107 193 265 375 # 900 1101 # # TspGWI: # [ACGGA] # 281 3205 # # TspRI: # [CASTGNN] # 72 72 72 72 72 90 # 105 129 141 156 195 222 # 288 657 1143 # # TstI: # [CACNNNNNNTCC] # 32 273 3181 # # VspI: # [ATTAAT] # 264 643 2579 # # XhoI: # [CTCGAG] # 1557 1929 # # XhoII: # [RGATCY] # 1529 1957 # # XmnI: # [GAANNNNTTC] # 1093 2393 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 3486 # Reported_sequences: 1 # Reported_hitcount: 727 #---------------------------------------