Restriction Fragments for DAN1/YJR150C



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

DAN1/YJR150C on chromosome X from coordinates 709707 to 708811.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.6883.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 4 9 + XbaI TCTAGA 4 8 . . 4 9 + Hpy178III* TCNNGA 5 7 . . 5 8 + MaeI CTAG 5 7 . . 9 12 + TspEI AATT 8 12 . . 21 24 + SetI ASST 24 20 . . 21 24 + CviJI RGCY 22 22 . . 21 24 + AluI AGCT 22 22 . . 22 32 + MwoI GCNNNNNNNGC 28 25 . . 28 32 + TauI GCSGC 31 28 . . 28 32 + BlsI GCNGC 30 29 . . 28 32 + BisI GCNGC 29 30 . . 29 32 + AciI CCGC 29 31 . . 31 35 + BlsI GCNGC 33 32 . . 31 35 + BisI GCNGC 32 33 . . 31 35 + BbvI GCAGC 43 47 . . 31 35 + TseI GCWGC 31 34 . . 34 44 + MwoI GCNNNNNNNGC 40 37 . . 48 51 + CviRI* TGCA 49 49 . . 49 59 + MwoI GCNNNNNNNGC 55 52 . . 53 56 + AciI CCGC 53 55 . . 55 59 + TauI GCSGC 58 55 . . 55 59 + BlsI GCNGC 57 56 . . 55 59 + BisI GCNGC 56 57 . . 56 59 + AciI CCGC 56 58 . . 58 62 + SfaNI GCATC 67 71 . . 59 63 - FokI GGATG 45 49 . . 59 63 - BseGI GGATG 58 56 . . 64 68 + Tsp45I GTSAC 63 68 . . 64 68 + MaeIII GTNAC 63 68 . . 61 65 - TspGWI ACGGA 51 49 . . 66 76 + Hin4I GAYNNNNNVTC 57 52 89 84 89 93 + TspDTI ATGAA 104 102 . . 100 103 + TspEI AATT 99 103 . . 110 113 + TspEI AATT 109 113 . . 114 117 + CviJI RGCY 115 115 . . 118 123 + Hpy166II GTNNAC 120 120 . . 122 125 + TaiI ACGT 125 121 . . 122 125 + SetI ASST 125 121 . . 122 125 + MaeII ACGT 122 124 . . 127 131 + Hpy188I TCNGA 129 128 . . 136 141 + NarI GGCGCC 137 139 . . 136 141 + KasI GGCGCC 136 140 . . 136 141 + DinI GGCGCC 138 138 . . 136 141 + HaeII RGCGCY 140 136 . . 136 141 + NlaIV GGNNCC 138 138 . . 136 141 + AcyI GRCGYC 137 139 . . 136 141 + HgiCI* GGYRCC 136 140 . . 137 140 + Hin6I GCGC 137 139 . . 137 140 + GlaI GCGC 138 138 . . 137 140 + HhaI GCGC 139 137 . . 148 152 + Hpy188I TCNGA 150 149 . . 152 157 + SspI AATATT 154 154 . . 160 170 + MwoI GCNNNNNNNGC 166 163 . . 165 169 + BssKI CCNGG 164 169 . . 165 169 + BseBI CCWGG 166 167 . . 165 169 + ScrFI CCNGG 166 167 . . 165 169 + EcoRII CCWGG 164 169 . . 168 171 + CviJI RGCY 169 169 . . 173 176 + CviRI* TGCA 174 174 . . 195 198 + SetI ASST 198 194 . . 201 204 + TspEI AATT 200 204 . . 206 211 + StyI CCWWGG 206 210 . . 206 211 + SecI* CCNNGG 206 210 . . 210 213 + CviJI RGCY 211 211 . . 211 221 + MwoI GCNNNNNNNGC 217 214 . . 220 225 + Cfr10I RCCGGY 220 224 . . 221 224 + HpaII CCGG 221 223 . . 234 243 + MslI CAYNNNNRTG 238 238 . . 233 237 - HphI GGTGA 225 224 . . 240 243 + FatI CATG 239 243 . . 240 243 + NlaIII CATG 243 239 . . 240 243 + CviAII CATG 240 242 . . 243 248 + Hpy166II GTNNAC 245 245 . . 243 248 + HindII GTYRAC 245 245 . . 247 251 + BsrI ACTGG 252 250 . . 258 261 + SetI ASST 261 257 . . 259 263 + HphI GGTGA 271 270 . . 263 267 + TspDTI ATGAA 278 276 . . 268 272 + Tsp45I GTSAC 267 272 . . 268 272 + MaeIII GTNAC 267 272 . . 278 283 + BclI TGATCA 278 282 . . 279 282 + DpnI GATC 280 280 . . 279 282 + BstKTI GATC 281 279 . . 279 282 + MboI GATC 278 282 . . 282 289 + SgrAI CRCCGGYG 283 287 . . 282 291 + MslI CAYNNNNRTG 286 286 . . 282 291 + OliI CACNNNNGTG 286 286 . . 283 288 + Cfr10I RCCGGY 283 287 . . 283 288 + BetI* WCCGGW 283 287 . . 283 288 + AgeI ACCGGT 283 287 . . 284 287 + HpaII CCGG 284 286 . . 281 285 - HphI GGTGA 273 272 . . 292 297 + DsaI* CCRYGG 292 296 . . 292 297 + StyI CCWWGG 292 296 . . 292 297 + NcoI CCATGG 292 296 . . 292 297 + SecI* CCNNGG 292 296 . . 293 296 + FatI CATG 292 296 . . 293 296 + NlaIII CATG 296 292 . . 293 296 + CviAII CATG 293 295 . . 297 300 + Csp6I GTAC 297 299 . . 297 300 + RsaI GTAC 298 298 . . 305 308 + MaeI CTAG 305 307 . . 312 316 - BccI CCATC 306 307 . . 314 319 - TaqII CACCCA 304 302 . . 325 329 + Hpy188I TCNGA 327 326 . . 327 338 + BcgI CGANNNNNNTGC 316 314 350 348 341 345 + TspDTI ATGAA 356 354 . . 345 348 + SetI ASST 348 344 . . 343 347 - Hin4II* CCTTC 337 336 . . 355 365 + BaeI ACNNNNGTAYC 344 339 377 372 356 361 + PstI CTGCAG 360 356 . . 356 361 + SfeI* CTRYAG 356 360 . . 357 360 + CviRI* TGCA 358 358 . . 358 368 + MwoI GCNNNNNNNGC 364 361 . . 361 364 + Csp6I GTAC 361 363 . . 361 364 + RsaI GTAC 362 362 . . 366 369 + SetI ASST 369 365 . . 366 369 + CviJI RGCY 367 367 . . 366 369 + AluI AGCT 367 367 . . 381 384 + CviJI RGCY 382 382 . . 379 382 - MnlI CCTC 372 371 . . 385 389 - MboII GAAGA 377 376 . . 391 394 + SetI ASST 394 390 . . 392 395 + MnlI CCTC 402 401 . . 400 404 + Hpy188I TCNGA 402 401 . . 398 403 - Eco57MI CTGRAG 383 381 . . 398 403 - Eco57I CTGAAG 383 381 . . 404 409 + HindIII AAGCTT 404 408 . . 405 408 + SetI ASST 408 404 . . 405 408 + CviJI RGCY 406 406 . . 405 408 + AluI AGCT 406 406 . . 406 416 + MwoI GCNNNNNNNGC 412 409 . . 410 413 + MaeI CTAG 410 412 . . 415 419 + TauI GCSGC 418 415 . . 415 419 + BlsI GCNGC 417 416 . . 415 419 + BisI GCNGC 416 417 . . 416 419 + AciI CCGC 416 418 . . 420 426 + TspRI CASTGNN 426 417 . . 422 426 + DdeI CTNAG 422 425 . . 424 428 + PleI GAGTC 432 433 . . 424 428 + MlyI GAGTC 433 433 . . 424 428 + HinfI GANTC 424 427 . . 422 426 - BspCNI CTCAG 414 412 . . 422 426 - BseMII CTCAG 413 411 . . 427 431 - MboII GAAGA 419 418 . . 431 434 + MaeI CTAG 431 433 . . 426 431 - BbvII* GAAGAC 419 423 . . 433 436 + SetI ASST 436 432 . . 433 436 + CviJI RGCY 434 434 . . 433 436 + AluI AGCT 434 434 . . 437 440 + MnlI CCTC 447 446 . . 439 443 + Hpy188I TCNGA 441 440 . . 435 440 - BseRI GAGGAG 426 424 . . 449 454 + EcoP15I CAGCAG 479 481 . . 454 464 + BplI GAGNNNNNCTC 445 440 477 472 454 458 - BsmAI GTCTC 448 452 . . 454 459 - Eco31I GGTCTC 448 452 . . 460 463 + SetI ASST 463 459 . . 460 463 + CviJI RGCY 461 461 . . 460 463 + AluI AGCT 461 461 . . 454 464 - BplI GAGNNNNNCTC 477 472 445 440 469 473 + BlsI GCNGC 471 470 . . 469 473 + BisI GCNGC 470 471 . . 469 473 + TseI GCWGC 469 472 . . 469 473 - BbvI GCAGC 456 460 . . 477 482 + TaqII CACCCA 493 491 . . 483 486 + SetI ASST 486 482 . . 483 486 + CviJI RGCY 484 484 . . 483 486 + AluI AGCT 484 484 . . 487 491 + Tsp4CI* ACNGT 489 488 . . 492 496 - TspDTI ATGAA 482 480 . . 496 500 + Hpy188I TCNGA 498 497 . . 499 503 + TfiI GAWTC 499 502 . . 499 503 + HinfI GANTC 499 502 . . 503 506 + MaeI CTAG 503 505 . . 505 508 + SetI ASST 508 504 . . 505 508 + CviJI RGCY 506 506 . . 505 508 + AluI AGCT 506 506 . . 511 515 + BlsI GCNGC 513 512 . . 511 515 + BisI GCNGC 512 513 . . 511 515 + TseI GCWGC 511 514 . . 513 516 + CviRI* TGCA 514 514 . . 509 514 - EcoP15I CAGCAG 481 483 . . 514 519 + Cac8I GCNNGC 516 516 . . 511 515 - BbvI GCAGC 498 502 . . 525 528 + CviRI* TGCA 526 526 . . 526 536 + MwoI GCNNNNNNNGC 532 529 . . 526 536 + BstAPI GCANNNNNTGC 532 529 . . 522 527 - BsrDI GCAATG 521 519 . . 533 538 - EcoP15I CAGCAG 505 507 . . 549 552 + CviRI* TGCA 550 550 . . 550 555 + Cac8I GCNNGC 552 552 . . 553 556 + SetI ASST 556 552 . . 553 556 + CviJI RGCY 554 554 . . 553 556 + AluI AGCT 554 554 . . 561 564 + CviRI* TGCA 562 562 . . 562 567 + Cac8I GCNNGC 564 564 . . 565 568 + SetI ASST 568 564 . . 565 568 + CviJI RGCY 566 566 . . 565 568 + AluI AGCT 566 566 . . 573 576 + CviRI* TGCA 574 574 . . 574 584 + MwoI GCNNNNNNNGC 580 577 . . 574 579 + Cac8I GCNNGC 576 576 . . 574 584 + BstAPI GCANNNNNTGC 580 577 . . 577 580 + SetI ASST 580 576 . . 577 580 + CviJI RGCY 578 578 . . 577 580 + AluI AGCT 578 578 . . 583 593 + MwoI GCNNNNNNNGC 589 586 . . 584 587 + MaeI CTAG 584 586 . . 598 608 + BsaXI ACNNNNNCTCC 588 585 618 615 603 606 + SetI ASST 606 602 . . 603 606 + CviJI RGCY 604 604 . . 603 606 + AluI AGCT 604 604 . . 605 610 - Eco57MI CTGRAG 590 588 . . 605 610 - GsuI CTGGAG 590 588 . . 607 611 - BsrI ACTGG 607 605 . . 613 617 - MboII GAAGA 605 604 . . 622 626 - MboII GAAGA 614 613 . . 630 634 + DdeI CTNAG 630 633 . . 630 634 + BspCNI CTCAG 643 641 . . 630 634 + BseMII CTCAG 644 642 . . 634 638 + Tsp45I GTSAC 633 638 . . 634 638 + MaeIII GTNAC 633 638 . . 636 645 + MslI CAYNNNNRTG 640 640 . . 636 645 + OliI CACNNNNGTG 640 640 . . 641 647 + TspRI CASTGNN 647 638 . . 638 643 - Eco57MI CTGRAG 623 621 . . 638 643 - GsuI CTGGAG 623 621 . . 640 644 - BsrI ACTGG 640 638 . . 645 649 + BmgT120I GGNCC 646 647 . . 645 649 + AvaII GGWCC 645 648 . . 645 649 + AsuI* GGNCC 645 648 . . 656 659 + Csp6I GTAC 656 658 . . 656 659 + RsaI GTAC 657 657 . . 661 672 + DrdI GACNNNNNNGTC 667 665 . . 667 671 + Tsp4CI* ACNGT 669 668 . . 670 674 + Tsp45I GTSAC 669 674 . . 670 674 + MaeIII GTNAC 669 674 . . 678 682 + HinfI GANTC 678 681 . . 680 685 + SmlI CTYRAG 680 684 . . 678 682 - PleI GAGTC 672 673 . . 678 682 - MlyI GAGTC 672 672 . . 683 688 + HindIII AAGCTT 683 687 . . 684 687 + SetI ASST 687 683 . . 684 687 + CviJI RGCY 685 685 . . 684 687 + AluI AGCT 685 685 . . 680 685 - Bce83I* CTTGAG 665 663 . . 690 696 + TspRI CASTGNN 696 687 . . 691 695 + Tsp4CI* ACNGT 693 692 . . 709 714 + SpeI ACTAGT 709 713 . . 710 713 + MaeI CTAG 710 712 . . 725 728 + TaiI ACGT 728 724 . . 725 728 + SetI ASST 728 724 . . 725 728 + MaeII ACGT 725 727 . . 736 740 + Tsp4CI* ACNGT 738 737 . . 739 743 + Tsp45I GTSAC 738 743 . . 739 743 + MaeIII GTNAC 738 743 . . 743 747 + DdeI CTNAG 743 746 . . 746 749 + CviJI RGCY 747 747 . . 751 755 + BsmAI GTCTC 756 760 . . 748 752 - BsrI ACTGG 748 746 . . 755 758 + MnlI CCTC 765 764 . . 753 758 - BseRI GAGGAG 744 742 . . 762 765 + SetI ASST 765 761 . . 762 765 + CviJI RGCY 763 763 . . 762 765 + AluI AGCT 763 763 . . 770 775 + SfeI* CTRYAG 770 774 . . 774 777 + SetI ASST 777 773 . . 774 777 + CviJI RGCY 775 775 . . 774 777 + AluI AGCT 775 775 . . 784 788 + Tsp45I GTSAC 783 788 . . 784 788 + MaeIII GTNAC 783 788 . . 796 800 + SfaNI GCATC 805 809 . . 800 805 - BsiI* CACGAG 800 804 . . 812 815 + TaiI ACGT 815 811 . . 812 815 + SetI ASST 815 811 . . 812 815 + MaeII ACGT 812 814 . . 814 818 + MaeIII GTNAC 813 818 . . 824 828 + Tsp4CI* ACNGT 826 825 . . 845 849 + Tsp4CI* ACNGT 847 846 . . 856 861 + NlaIV GGNNCC 858 858 . . 856 861 + HgiCI* GGYRCC 856 860 . . 859 863 + TauI GCSGC 862 859 . . 859 863 + BlsI GCNGC 861 860 . . 859 863 + BisI GCNGC 860 861 . . 860 865 + NspBII* CMGCKG 862 862 . . 860 863 + AciI CCGC 860 862 . . 862 872 + MwoI GCNNNNNNNGC 868 865 . . 862 866 + BlsI GCNGC 864 863 . . 862 866 + BisI GCNGC 863 864 . . 862 866 + TseI GCWGC 862 865 . . 862 866 - BbvI GCAGC 849 853 . . 871 881 + MwoI GCNNNNNNNGC 877 874 . . 874 879 + NlaIV GGNNCC 876 876 . . 874 879 + HgiCI* GGYRCC 874 878 . . 877 881 + TauI GCSGC 880 877 . . 877 881 + BlsI GCNGC 879 878 . . 877 881 + BisI GCNGC 878 879 . . 878 881 + AciI CCGC 878 880 . . 880 884 + TauI GCSGC 883 880 . . 880 884 + BlsI GCNGC 882 881 . . 880 884 + BisI GCNGC 881 882 . . 881 884 + AciI CCGC 881 883 . . 881 886 + BsrBI CCGCTC 883 883 . . #--------------------------------------- # # # AciI: # [CCGC] # 3 3 16 18 24 29 # 360 444 # # AcyI: # [GRCGYC] # 137 760 # # AgeI: # [ACCGGT] # 283 614 # # AluI: # [AGCT] # 12 12 12 22 22 23 # 26 27 28 39 48 78 # 81 122 345 # # AsuI*: # [GGNCC] # 252 645 # # AvaII: # [GGWCC] # 252 645 # # BaeI: # [ACNNNNGTAYC] # 33 344 520 # # BbvI: # [GCAGC] # 42 43 48 351 413 # # BbvII*: # [GAAGAC] # 419 478 # # BccI: # [CCATC] # 306 591 # # Bce83I*: # [CTTGAG] # 232 665 # # BcgI: # [CGANNNNNNTGC] # 34 316 547 # # BclI: # [TGATCA] # 278 619 # # BetI*: # [WCCGGW] # 283 614 # # BisI: # [GCNGC] # 3 3 3 15 16 24 # 29 42 54 348 360 # # BlsI: # [GCNGC] # 3 3 3 15 15 24 # 30 42 54 348 360 # # BmgT120I: # [GGNCC] # 251 646 # # BplI: # [GAGNNNNNCTC] # 0 0 32 420 445 # # BsaXI: # [ACNNNNNCTCC] # 30 279 588 # # BseBI: # [CCWGG] # 166 731 # # BseGI: # [GGATG] # 58 839 # # BseMII: # [CTCAG] # 231 253 413 # # BseRI: # [GAGGAG] # 153 318 426 # # BsiI*: # [CACGAG] # 97 800 # # BsmAI: # [GTCTC] # 141 308 448 # # BspCNI: # [CTCAG] # 229 254 414 # # BsrBI: # [CCGCTC] # 14 883 # # BsrDI: # [GCAATG] # 376 521 # # BsrI: # [ACTGG] # 33 108 149 252 355 # # BssKI: # [CCNGG] # 164 733 # # BstAPI: # [GCANNNNNTGC] # 48 317 532 # # BstKTI: # [GATC] # 281 616 # # Cac8I: # [GCNNGC] # 12 12 36 321 516 # # Cfr10I: # [RCCGGY] # 63 220 614 # # Csp6I: # [GTAC] # 64 241 295 297 # # CviAII: # [CATG] # 53 240 604 # # CviJI: # [RGCY] # 12 12 12 15 16 22 # 22 23 24 26 27 28 # 42 48 54 62 81 93 # 122 156 # # CviRI*: # [TGCA] # 12 12 12 24 49 125 # 156 184 323 # # DdeI: # [CTNAG] # 113 154 208 422 # # DinI: # [GGCGCC] # 138 759 # # DpnI: # [GATC] # 280 617 # # DrdI: # [GACNNNNNNGTC] # 230 667 # # DsaI*: # [CCRYGG] # 292 605 # # Eco31I: # [GGTCTC] # 448 449 # # Eco57I: # [CTGAAG] # 383 514 # # Eco57MI: # [CTGRAG] # 33 207 274 383 # # EcoP15I: # [CAGCAG] # 2 24 392 479 # # EcoRII: # [CCWGG] # 164 733 # # FatI: # [CATG] # 53 239 605 # # FokI: # [GGATG] # 45 852 # # GlaI: # [GCGC] # 138 759 # # GsuI: # [CTGGAG] # 33 274 590 # # HaeII: # [RGCGCY] # 140 757 # # HgiCI*: # [GGYRCC] # 18 23 136 720 # # HhaI: # [GCGC] # 139 758 # # Hin4I: # [GAYNNNNNVTC] # 32 57 808 # # Hin4II*: # [CCTTC] # 337 560 # # Hin6I: # [GCGC] # 137 760 # # HindII: # [GTYRAC] # 245 652 # # HindIII: # [AAGCTT] # 214 279 404 # # HinfI: # [GANTC] # 75 179 219 424 # # HpaII: # [CCGG] # 63 221 613 # # HphI: # [GGTGA] # 2 46 225 624 # # Hpy166II: # [GTNNAC] # 120 125 652 # # Hpy178III*: # [TCNNGA] # 5 892 # # Hpy188I: # [TCNGA] # 21 39 57 75 129 177 # 399 # # KasI: # [GGCGCC] # 136 761 # # MaeI: # [CTAG] # 5 21 72 81 105 126 # 187 300 # # MaeII: # [ACGT] # 85 87 122 603 # # MaeIII: # [GTNAC] # 30 36 45 63 69 84 # 204 366 # # MboI: # [GATC] # 278 619 # # MboII: # [GAAGA] # 9 42 186 283 377 # # MlyI: # [GAGTC] # 225 239 433 # # MnlI: # [CCTC] # 30 45 132 318 372 # # MslI: # [CAYNNNNRTG] # 48 238 257 354 # # MwoI: # [GCNNNNNNNGC] # 9 9 12 15 20 28 # 48 48 51 111 120 147 # 279 # # NarI: # [GGCGCC] # 137 760 # # NcoI: # [CCATGG] # 292 605 # # NlaIII: # [CATG] # 53 243 601 # # NlaIV: # [GGNNCC] # 18 21 138 720 # # NspBII*: # [CMGCKG] # 35 862 # # OliI: # [CACNNNNGTG] # 257 286 354 # # PleI: # [GAGTC] # 225 240 432 # # PstI: # [CTGCAG] # 360 537 # # RsaI: # [GTAC] # 64 240 295 298 # # ScrFI: # [CCNGG] # 166 731 # # SecI*: # [CCNNGG] # 86 206 605 # # SetI: # [ASST] # 12 12 12 14 21 22 # 23 24 25 26 27 28 # 37 38 41 48 63 73 # 81 82 87 101 # # SfaNI: # [GCATC] # 67 92 738 # # SfeI*: # [CTRYAG] # 127 356 414 # # SgrAI: # [CRCCGGYG] # 283 614 # # SmlI: # [CTYRAG] # 217 680 # # SpeI: # [ACTAGT] # 188 709 # # SspI: # [AATATT] # 154 743 # # StyI: # [CCWWGG] # 86 206 605 # # TaiI: # [ACGT] # 82 87 125 603 # # TaqII: # [CACCCA] # 189 304 404 # # TauI: # [GCSGC] # 3 14 18 27 31 360 # 444 # # TfiI: # [GAWTC] # 398 499 # # TseI: # [GCWGC] # 31 35 42 351 438 # # Tsp45I: # [GTSAC] # 36 45 63 69 114 204 # 366 # # Tsp4CI*: # [ACNGT] # 21 24 45 50 88 180 # 489 # # TspDTI: # [ATGAA] # 78 104 126 174 415 # # TspEI: # [AATT] # 8 10 91 91 697 # # TspGWI: # [ACGGA] # 51 846 # # TspRI: # [CASTGNN] # 49 201 221 426 # # XbaI: # [TCTAGA] # 4 893 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 897 # Reported_sequences: 1 # Reported_hitcount: 304 #---------------------------------------