Restriction Fragments for BAT2/YJR148W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

BAT2/YJR148W on chromosome X from coordinates 705744 to 706874.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.8401.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 4 7 + SetI ASST 7 3 . . 5 10 + StyI CCWWGG 5 9 . . 5 10 + SecI* CCNNGG 5 9 . . 9 14 + NlaIV GGNNCC 11 11 . . 9 14 + HgiCI* GGYRCC 9 13 . . 16 19 + MaeI CTAG 16 18 . . 19 23 + HgaI GACGC 28 33 . . 19 24 + AcyI GRCGYC 20 22 . . 23 26 + MnlI CCTC 33 32 . . 32 35 + MseI TTAA 32 34 . . 51 56 + NspI RCATGY 55 51 . . 52 55 + FatI CATG 51 55 . . 52 55 + NlaIII CATG 55 51 . . 52 55 + CviAII CATG 52 54 . . 53 58 + EcoT22I ATGCAT 57 53 . . 54 57 + CviRI* TGCA 55 55 . . 55 59 + SfaNI GCATC 64 68 . . 59 63 + DdeI CTNAG 59 62 . . 62 65 + CviJI RGCY 63 63 . . 74 78 + Tsp4CI* ACNGT 76 75 . . 75 81 + TspRI CASTGNN 81 72 . . 97 100 + SetI ASST 100 96 . . 97 100 + CviJI RGCY 98 98 . . 97 100 + AluI AGCT 98 98 . . 103 107 + TspGWI ACGGA 118 116 . . 105 109 + BmgT120I GGNCC 106 107 . . 105 109 + AvaII GGWCC 105 108 . . 105 109 + AsuI* GGNCC 105 108 . . 110 115 + BspLU11I* ACATGT 110 114 . . 110 115 + NspI RCATGY 114 110 . . 110 115 + AflIII ACRYGT 110 114 . . 111 114 + FatI CATG 110 114 . . 111 114 + NlaIII CATG 114 110 . . 111 114 + CviAII CATG 111 113 . . 114 119 + Hpy166II GTNNAC 116 116 . . 114 119 + HpaI GTTAAC 116 116 . . 114 119 + HindII GTYRAC 116 116 . . 115 118 + MseI TTAA 115 117 . . 121 124 - AciI CCGC 121 123 . . 131 136 + PvuII CAGCTG 133 133 . . 131 136 + NspBII* CMGCKG 133 133 . . 132 135 + SetI ASST 135 131 . . 132 135 + CviJI RGCY 133 133 . . 132 135 + AluI AGCT 133 133 . . 148 153 + KpnI GGTACC 152 148 . . 148 153 + NlaIV GGNNCC 150 150 . . 148 153 + HgiCI* GGYRCC 148 152 . . 148 153 + Acc65I GGTACC 148 152 . . 149 152 + Csp6I GTAC 149 151 . . 149 152 + RsaI GTAC 150 150 . . 161 164 + MseI TTAA 161 163 . . 165 168 + SetI ASST 168 164 . . 189 199 + BsiYI* CCNNNNNNNGG 195 192 . . 190 194 + Hin4II* CCTTC 200 199 . . 194 199 + NspBII* CMGCKG 196 196 . . 194 199 + SacII CCGCGG 197 195 . . 194 199 + DsaI* CCRYGG 194 198 . . 194 199 + SecI* CCNNGG 194 198 . . 194 197 + AciI CCGC 194 196 . . 195 198 + FnuDII* CGCG 196 196 . . 196 199 - AciI CCGC 196 198 . . 221 224 + SetI ASST 224 220 . . 221 224 + CviJI RGCY 222 222 . . 221 224 + AluI AGCT 222 222 . . 226 231 + AsuII TTCGAA 227 229 . . 227 230 + TaqI TCGA 227 229 . . 229 233 - Hin4II* CCTTC 223 222 . . 233 237 + FokI GGATG 246 250 . . 233 237 + BseGI GGATG 239 237 . . 235 239 + TspDTI ATGAA 250 248 . . 240 243 + CviJI RGCY 241 241 . . 237 241 - Hin4II* CCTTC 231 230 . . 250 254 + Tsp4CI* ACNGT 252 251 . . 253 258 + Hpy166II GTNNAC 255 255 . . 264 267 + TspEI AATT 263 267 . . 279 284 + Hpy178III* TCNNGA 280 282 . . 286 290 + TspDTI ATGAA 301 299 . . 292 296 + TspDTI ATGAA 307 305 . . 297 300 + Hin6I GCGC 297 299 . . 297 300 + GlaI GCGC 298 298 . . 297 300 + HhaI GCGC 299 297 . . 300 303 + FatI CATG 299 303 . . 300 303 + NlaIII CATG 303 299 . . 300 303 + CviAII CATG 300 302 . . 301 305 + TspDTI ATGAA 316 314 . . 314 318 + DdeI CTNAG 314 317 . . 314 318 + BspCNI CTCAG 327 325 . . 314 318 + BseMII CTCAG 328 326 . . 315 319 + Hpy188I TCNGA 317 316 . . 320 324 + TfiI GAWTC 320 323 . . 320 324 + HinfI GANTC 320 323 . . 333 338 + AclI AACGTT 334 336 . . 334 337 + TaiI ACGT 337 333 . . 334 337 + SetI ASST 337 333 . . 334 337 + MaeII ACGT 334 336 . . 338 341 + TaqI TCGA 338 340 . . 346 350 + MboII GAAGA 358 357 . . 346 351 - Ksp632I* CTCTTC 341 344 . . 359 369 + BsiYI* CCNNNNNNNGG 365 362 . . 363 366 + TspEI AATT 362 366 . . 375 378 + DpnI GATC 376 376 . . 375 378 + BstKTI GATC 377 375 . . 375 378 + MboI GATC 374 378 . . 375 379 - BinI* GGATC 369 370 . . 393 397 + DdeI CTNAG 393 396 . . 399 404 + Hpy178III* TCNNGA 400 402 . . 401 406 + Eco57MI CTGRAG 422 420 . . 401 406 + Eco57I CTGAAG 422 420 . . 403 407 - Hin4II* CCTTC 397 396 . . 411 414 + SetI ASST 414 410 . . 413 417 + MaeIII GTNAC 412 417 . . 430 435 + StuI AGGCCT 432 432 . . 431 434 + CviJI RGCY 432 432 . . 431 434 + HaeIII GGCC 432 432 . . 438 443 + VspI ATTAAT 439 441 . . 439 442 + MseI TTAA 439 441 . . 451 455 + BceAI ACGGC 467 469 . . 452 457 + McrI* CGRYCG 455 453 . . 452 457 + XmaIII* CGGCCG 452 456 . . 452 457 + CfrI YGGCCR 452 456 . . 453 456 + CviJI RGCY 454 454 . . 453 456 + HaeIII GGCC 454 454 . . 454 459 + Cfr10I RCCGGY 454 458 . . 455 458 + HpaII CCGG 455 457 . . 455 465 + BsiYI* CCNNNNNNNGG 461 458 . . 483 486 + CviJI RGCY 484 484 . . 503 507 + BlsI GCNGC 505 504 . . 503 507 + BisI GCNGC 504 505 . . 503 507 + TseI GCWGC 503 506 . . 503 507 - BbvI GCAGC 490 494 . . 513 519 + PpuMI RGGWCCY 514 517 . . 513 519 + DraII RGGNCCY 514 517 . . 513 518 + NlaIV GGNNCC 515 515 . . 514 518 + BmgT120I GGNCC 515 516 . . 514 518 + AvaII GGWCC 514 517 . . 514 518 + AsuI* GGNCC 514 517 . . 529 533 + BsrI ACTGG 534 532 . . 536 539 + MseI TTAA 536 538 . . 541 544 - AciI CCGC 541 543 . . 545 549 + Hpy188I TCNGA 547 546 . . 549 553 + BsrI ACTGG 554 552 . . 555 558 + CviJI RGCY 556 556 . . 558 564 + TspRI CASTGNN 564 555 . . 568 578 + MwoI GCNNNNNNNGC 574 571 . . 576 579 + SetI ASST 579 575 . . 576 579 + CviJI RGCY 577 577 . . 576 579 + AluI AGCT 577 577 . . 580 585 + BalI TGGCCA 582 582 . . 580 585 + CfrI YGGCCR 580 584 . . 581 584 + CviJI RGCY 582 582 . . 581 584 + HaeIII GGCC 582 582 . . 583 587 + BssKI CCNGG 582 587 . . 583 587 + BseBI CCWGG 584 585 . . 583 587 + ScrFI CCNGG 584 585 . . 583 587 + EcoRII CCWGG 582 587 . . 589 592 + CviJI RGCY 590 590 . . 587 590 - MnlI CCTC 580 579 . . 595 599 + HphI GGTGA 607 606 . . 596 600 + Tsp45I GTSAC 595 600 . . 596 600 + MaeIII GTNAC 595 600 . . 607 610 + MaeI CTAG 607 609 . . 609 612 + SetI ASST 612 608 . . 612 615 + CviRI* TGCA 613 613 . . 613 623 + MwoI GCNNNNNNNGC 619 616 . . 629 633 - HgaI GACGC 618 623 . . 636 646 + MwoI GCNNNNNNNGC 642 639 . . 640 645 + MfeI CAATTG 640 644 . . 641 644 + TspEI AATT 640 644 . . 644 647 + CviRI* TGCA 645 645 . . 645 650 + Cac8I GCNNGC 647 647 . . 648 651 + SetI ASST 651 647 . . 648 651 + CviJI RGCY 649 649 . . 648 651 + AluI AGCT 649 649 . . 649 653 + BlsI GCNGC 651 650 . . 649 653 + BisI GCNGC 650 651 . . 649 653 + TseI GCWGC 649 652 . . 649 653 - BbvI GCAGC 636 640 . . 661 667 + BstEII GGTNACC 661 666 . . 662 666 + MaeIII GTNAC 661 666 . . 672 677 + ApoI RAATTY 672 676 . . 673 676 + TspEI AATT 672 676 . . 680 683 + CviJI RGCY 681 681 . . 679 684 - TsoI TARCCA 669 667 . . 688 692 + BmgT120I GGNCC 689 690 . . 688 692 + AvaII GGWCC 688 691 . . 688 692 + AsuI* GGNCC 688 691 . . 707 712 + Eco57MI CTGRAG 728 726 . . 707 712 + Eco57I CTGAAG 728 726 . . 715 720 + NlaIV GGNNCC 717 717 . . 715 720 + HgiCI* GGYRCC 715 719 . . 720 723 + FatI CATG 719 723 . . 720 723 + NlaIII CATG 723 719 . . 720 723 + CviAII CATG 720 722 . . 721 725 + TspDTI ATGAA 736 734 . . 723 728 + BsmI GAATGC 729 727 . . 739 744 + AhaIII* TTTAAA 741 741 . . 740 743 + MseI TTAA 740 742 . . 762 766 - Hin4II* CCTTC 756 755 . . 768 773 + SpeI ACTAGT 768 772 . . 769 772 + MaeI CTAG 769 771 . . 772 776 + MaeIII GTNAC 771 776 . . 781 791 + BsiYI* CCNNNNNNNGG 787 784 . . 784 787 + MaeI CTAG 784 786 . . 788 792 + Tsp4CI* ACNGT 790 789 . . 790 795 + KpnI GGTACC 794 790 . . 790 795 + NlaIV GGNNCC 792 792 . . 790 795 + HgiCI* GGYRCC 790 794 . . 790 795 + Acc65I GGTACC 790 794 . . 791 794 + Csp6I GTAC 791 793 . . 791 794 + RsaI GTAC 792 792 . . 804 807 + SetI ASST 807 803 . . 802 806 - Hin4II* CCTTC 796 795 . . 808 812 + MaeIII GTNAC 807 812 . . 812 815 + MaeI CTAG 812 814 . . 817 821 + TfiI GAWTC 817 820 . . 817 821 + HinfI GANTC 817 820 . . 825 830 + AhaIII* TTTAAA 827 827 . . 826 829 + MseI TTAA 826 828 . . 845 849 + HinfI GANTC 845 848 . . 848 851 + TaqI TCGA 848 850 . . 845 849 - PleI GAGTC 839 840 . . 845 849 - MlyI GAGTC 839 839 . . 853 864 + BstXI CCANNNNNNTGG 860 856 . . 863 867 + BmgT120I GGNCC 864 865 . . 863 867 + AvaII GGWCC 863 866 . . 863 867 + AsuI* GGNCC 863 866 . . 872 877 + Hpy166II GTNNAC 874 874 . . 863 874 - TstI CACNNNNNNTCC 887 882 855 850 887 892 + SfeI* CTRYAG 887 891 . . 898 902 + MaeIII GTNAC 897 902 . . 902 906 + DdeI CTNAG 902 905 . . 902 906 - BspCNI CTCAG 894 892 . . 902 906 - BseMII CTCAG 893 891 . . 907 912 + XhoII RGATCY 907 911 . . 908 911 + DpnI GATC 909 909 . . 908 911 + BstKTI GATC 910 908 . . 908 911 + MboI GATC 907 911 . . 908 912 - BinI* GGATC 902 903 . . 917 921 + Tsp4CI* ACNGT 919 918 . . 919 923 + HphI GGTGA 931 930 . . 920 925 + Hpy166II GTNNAC 922 922 . . 933 936 + CviJI RGCY 934 934 . . 947 950 + Csp6I GTAC 947 949 . . 947 950 + RsaI GTAC 948 948 . . 952 956 + BlsI GCNGC 954 953 . . 952 956 + BisI GCNGC 953 954 . . 952 956 + TseI GCWGC 952 955 . . 950 955 - EcoP15I CAGCAG 922 924 . . 952 956 - BbvI GCAGC 939 943 . . 957 967 + Hin4I GAYNNNNNVTC 948 943 980 975 971 974 + MseI TTAA 971 973 . . 982 985 + CviJI RGCY 983 983 . . 983 993 + MwoI GCNNNNNNNGC 989 986 . . 999 1002 + TspEI AATT 998 1002 . . 1000 1005 + VspI ATTAAT 1001 1003 . . 1001 1004 + MseI TTAA 1001 1003 . . 1003 1008 + SspI AATATT 1005 1005 . . 1008 1012 - TspGWI ACGGA 998 996 . . 1018 1022 + BssKI CCNGG 1017 1022 . . 1018 1022 + ScrFI CCNGG 1019 1020 . . 1018 1022 + CauII* CCSGG 1019 1020 . . 1019 1022 + HpaII CCGG 1019 1021 . . 1030 1035 + Cfr10I RCCGGY 1030 1034 . . 1030 1035 + BetI* WCCGGW 1030 1034 . . 1030 1035 + AgeI ACCGGT 1030 1034 . . 1031 1034 + HpaII CCGG 1031 1033 . . 1033 1037 + BmgT120I GGNCC 1034 1035 . . 1033 1037 + AvaII GGWCC 1033 1036 . . 1033 1037 + AsuI* GGNCC 1033 1036 . . 1040 1045 + BalI TGGCCA 1042 1042 . . 1040 1045 + CfrI YGGCCR 1040 1044 . . 1041 1044 + CviJI RGCY 1042 1042 . . 1041 1044 + HaeIII GGCC 1042 1042 . . 1053 1056 + CviRI* TGCA 1054 1054 . . 1063 1068 + VspI ATTAAT 1064 1066 . . 1064 1067 + MseI TTAA 1064 1066 . . 1070 1074 + TfiI GAWTC 1070 1073 . . 1070 1074 + HinfI GANTC 1070 1073 . . 1088 1092 + DdeI CTNAG 1088 1091 . . 1084 1088 - BsmAI GTCTC 1078 1082 . . 1088 1092 - BspCNI CTCAG 1080 1078 . . 1088 1092 - BseMII CTCAG 1079 1077 . . 1093 1096 + FatI CATG 1092 1096 . . 1093 1096 + NlaIII CATG 1096 1092 . . 1093 1096 + CviAII CATG 1093 1095 . . 1098 1103 + MfeI CAATTG 1098 1102 . . 1099 1102 + TspEI AATT 1098 1102 . . 1114 1118 + MaeIII GTNAC 1113 1118 . . #--------------------------------------- # # # Acc65I: # [GGTACC] # 148 341 642 # # AciI: # [CCGC] # 2 73 121 345 590 # # AclI: # [AACGTT] # 334 797 # # AcyI: # [GRCGYC] # 20 1111 # # AflIII: # [ACRYGT] # 110 1021 # # AgeI: # [ACCGGT] # 101 1030 # # AhaIII*: # [TTTAAA] # 86 304 741 # # AluI: # [AGCT] # 35 72 89 98 355 482 # # ApoI: # [RAATTY] # 459 672 # # AsuI*: # [GGNCC] # 98 105 170 174 175 409 # # AsuII: # [TTCGAA] # 227 904 # # AvaII: # [GGWCC] # 98 105 170 174 175 409 # # BalI: # [TGGCCA] # 89 460 582 # # BbvI: # [GCAGC] # 146 192 303 490 # # BceAI: # [ACGGC] # 467 664 # # BetI*: # [WCCGGW] # 101 1030 # # BinI*: # [GGATC] # 229 369 533 # # BisI: # [GCNGC] # 146 178 303 504 # # BlsI: # [GCNGC] # 146 177 303 505 # # BmgT120I: # [GGNCC] # 97 106 170 174 175 409 # # BseBI: # [CCWGG] # 547 584 # # BseGI: # [GGATG] # 239 892 # # BseMII: # [CTCAG] # 52 186 328 565 # # BsiYI*: # [CCNNNNNNNGG] # 96 170 195 326 344 # # BsmAI: # [GTCTC] # 53 1078 # # BsmI: # [GAATGC] # 402 729 # # BspCNI: # [CTCAG] # 51 186 327 567 # # BspLU11I*: # [ACATGT] # 110 1021 # # BsrI: # [ACTGG] # 20 534 577 # # BssKI: # [CCNGG] # 114 435 582 # # BstEII: # [GGTNACC] # 470 661 # # BstKTI: # [GATC] # 221 377 533 # # BstXI: # [CCANNNNNNTGG] # 271 860 # # Cac8I: # [GCNNGC] # 484 647 # # CauII*: # [CCSGG] # 112 1019 # # Cfr10I: # [RCCGGY] # 101 454 576 # # CfrI: # [YGGCCR] # 91 128 452 460 # # Csp6I: # [GTAC] # 149 156 184 642 # # CviAII: # [CATG] # 38 52 59 189 373 420 # # CviJI: # [RGCY] # 5 8 19 21 22 30 # 32 35 35 49 59 59 # 63 72 89 89 191 253 # # CviRI*: # [TGCA] # 32 55 77 409 558 # # DdeI: # [CTNAG] # 43 59 79 186 255 509 # # DpnI: # [GATC] # 222 376 533 # # DraII: # [RGGNCCY] # 514 617 # # DsaI*: # [CCRYGG] # 194 937 # # Eco57I: # [CTGAAG] # 306 403 422 # # Eco57MI: # [CTGRAG] # 306 403 422 # # EcoP15I: # [CAGCAG] # 209 922 # # EcoRII: # [CCWGG] # 549 582 # # EcoT22I: # [ATGCAT] # 57 1074 # # FatI: # [CATG] # 39 51 59 189 373 420 # # FnuDII*: # [CGCG] # 196 935 # # FokI: # [GGATG] # 246 885 # # GlaI: # [GCGC] # 298 833 # # HaeIII: # [GGCC] # 22 89 128 432 460 # # HgaI: # [GACGC] # 28 513 590 # # HgiCI*: # [GGYRCC] # 9 75 139 341 567 # # HhaI: # [GCGC] # 299 832 # # Hin4I: # [GAYNNNNNVTC] # 32 151 948 # # Hin4II*: # [CCTTC] # 8 23 40 166 200 335 # 359 # # Hin6I: # [GCGC] # 297 834 # # HindII: # [GTYRAC] # 116 1015 # # HinfI: # [GANTC] # 28 61 225 320 497 # # HpaI: # [GTTAAC] # 116 1015 # # HpaII: # [CCGG] # 12 100 455 564 # # HphI: # [GGTGA] # 200 324 607 # # Hpy166II: # [GTNNAC] # 48 116 139 209 619 # # Hpy178III*: # [TCNNGA] # 120 280 731 # # Hpy188I: # [TCNGA] # 230 317 584 # # KpnI: # [GGTACC] # 152 337 642 # # Ksp632I*: # [CTCTTC] # 341 790 # # MaeI: # [CTAG] # 15 16 28 162 319 591 # # MaeII: # [ACGT] # 334 797 # # MaeIII: # [GTNAC] # 18 36 66 90 110 183 # 216 412 # # MboI: # [GATC] # 224 374 533 # # MboII: # [GAAGA] # 358 773 # # McrI*: # [CGRYCG] # 455 676 # # MfeI: # [CAATTG] # 33 458 640 # # MlyI: # [GAGTC] # 292 839 # # MnlI: # [CCTC] # 33 547 551 # # MseI: # [TTAA] # 30 32 46 63 67 83 # 86 97 145 204 278 # # MwoI: # [GCNNNNNNNGC] # 23 45 142 347 574 # # NlaIII: # [CATG] # 35 55 59 189 373 420 # # NlaIV: # [GGNNCC] # 11 75 139 202 339 365 # # NspBII*: # [CMGCKG] # 63 133 935 # # NspI: # [RCATGY] # 55 59 1017 # # PleI: # [GAGTC] # 292 839 # # PpuMI: # [RGGWCCY] # 514 617 # # PvuII: # [CAGCTG] # 133 998 # # RsaI: # [GTAC] # 150 156 183 642 # # SacII: # [CCGCGG] # 197 934 # # ScrFI: # [CCNGG] # 112 435 584 # # SecI*: # [CCNNGG] # 5 189 937 # # SetI: # [ASST] # 7 33 33 35 39 56 # 77 93 113 156 165 324 # # SfaNI: # [GCATC] # 64 1067 # # SfeI*: # [CTRYAG] # 244 887 # # SpeI: # [ACTAGT] # 363 768 # # SspI: # [AATATT] # 126 1005 # # StuI: # [AGGCCT] # 432 699 # # StyI: # [CCWWGG] # 5 1126 # # TaiI: # [ACGT] # 337 794 # # TaqI: # [TCGA] # 111 227 283 510 # # TfiI: # [GAWTC] # 61 253 320 497 # # TseI: # [GCWGC] # 146 179 303 503 # # TsoI: # [TARCCA] # 462 669 # # Tsp45I: # [GTSAC] # 536 595 # # Tsp4CI*: # [ACNGT] # 76 129 176 212 538 # # TspDTI: # [ATGAA] # 6 9 51 250 395 420 # # TspEI: # [AATT] # 32 33 99 100 263 278 # 326 # # TspGWI: # [ACGGA] # 118 133 880 # # TspRI: # [CASTGNN] # 81 483 567 # # TstI: # [CACNNNNNNTCC] # 32 244 855 # # VspI: # [ATTAAT] # 63 67 439 562 # # XhoII: # [RGATCY] # 224 907 # # XmaIII*: # [CGGCCG] # 452 679 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1131 # Reported_sequences: 1 # Reported_hitcount: 288 #---------------------------------------