Restriction Fragments for RPS4A/YJR145C



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The currently selected gene/sequence is :

RPS4A/YJR145C on chromosome X from coordinates 703068 to 702027.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.2488.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 3 6 + CviJI RGCY 4 4 . . 5 8 + MaeI CTAG 5 7 . . 10 14 + BmgT120I GGNCC 11 12 . . 10 14 + AvaII GGWCC 10 13 . . 10 14 + AsuI* GGNCC 10 13 . . 12 16 + Tsp4CI* ACNGT 14 13 . . 25 30 + SfeI* CTRYAG 25 29 . . 38 43 + PsiI TTATAA 40 40 . . 42 45 + TspEI AATT 41 45 . . 52 56 + FokI GGATG 65 69 . . 52 56 + BseGI GGATG 58 56 . . 58 62 + MboII GAAGA 70 69 . . 84 88 + PleI GAGTC 92 93 . . 84 88 + MlyI GAGTC 93 93 . . 84 88 + HinfI GANTC 84 87 . . 89 92 + MseI TTAA 89 91 . . 90 95 + TsoI TARCCA 106 104 . . 113 117 + TspDTI ATGAA 128 126 . . 118 122 - BsrI ACTGG 118 116 . . 133 136 + MseI TTAA 133 135 . . 135 138 + TspEI AATT 134 138 . . 142 145 + DpnI GATC 143 143 . . 142 145 + BstKTI GATC 144 142 . . 142 145 + MboI GATC 141 145 . . 148 152 + BssKI CCNGG 147 152 . . 148 152 + BseBI CCWGG 149 150 . . 148 153 + SecI* CCNNGG 148 152 . . 148 152 + ScrFI CCNGG 149 150 . . 148 152 + EcoRII CCWGG 147 152 . . 145 149 - FokI GGATG 131 135 . . 145 149 - BseGI GGATG 144 142 . . 156 161 + TatI WGTACW 156 160 . . 156 161 + Bsp1407I TGTACA 156 160 . . 157 160 + Csp6I GTAC 157 159 . . 157 160 + RsaI GTAC 158 158 . . 162 167 + SspI AATATT 164 164 . . 190 195 + SduI GDGCHC 194 190 . . 190 195 + HgiAI* GWGCWC 194 190 . . 197 201 + TspDTI ATGAA 212 210 . . 220 224 - MboII GAAGA 212 211 . . 233 237 - TspDTI ATGAA 223 221 . . 265 268 + Csp6I GTAC 265 267 . . 265 268 + RsaI GTAC 266 266 . . 262 266 - TspGWI ACGGA 252 250 . . 277 281 + SfaNI GCATC 286 290 . . 293 297 + BlsI GCNGC 295 294 . . 293 297 + BisI GCNGC 294 295 . . 293 297 + BbvI GCAGC 305 309 . . 293 297 + TseI GCWGC 293 296 . . 295 298 + SetI ASST 298 294 . . 295 298 + CviJI RGCY 296 296 . . 295 298 + AluI AGCT 296 296 . . 299 310 + BstXI CCANNNNNNTGG 306 302 . . 305 311 + TspRI CASTGNN 311 302 . . 306 310 + BsrI ACTGG 311 309 . . 308 313 - TsoI TARCCA 298 296 . . 317 327 + Hin4I GAYNNNNNVTC 308 303 340 335 317 327 + Eam1105I GACNNNNNGTC 322 321 . . 326 331 + BetI* WCCGGW 326 330 . . 317 327 - Hin4I GAYNNNNNVTC 340 335 308 303 327 330 + HpaII CCGG 327 329 . . 333 337 + MaeIII GTNAC 332 337 . . 347 351 + BccI CCATC 355 356 . . 347 357 + BsiYI* CCNNNNNNNGG 353 350 . . 347 357 + PflMI CCANNNNNTGG 353 350 . . 351 356 - EcoP15I CAGCAG 323 325 . . 356 360 + BmgT120I GGNCC 357 358 . . 356 360 + AvaII GGWCC 356 359 . . 356 360 + AsuI* GGNCC 356 359 . . 357 362 + Hpy166II GTNNAC 359 359 . . 366 369 + TspEI AATT 365 369 . . 359 370 - CspCI CAANNNNNGTGG 383 381 348 346 374 378 + TfiI GAWTC 374 377 . . 374 378 + HinfI GANTC 374 377 . . 398 402 + DdeI CTNAG 398 401 . . 410 413 + MseI TTAA 410 412 . . 426 430 + BceAI ACGGC 442 444 . . 427 432 + McrI* CGRYCG 430 428 . . 427 432 + XmaIII* CGGCCG 427 431 . . 427 432 + CfrI YGGCCR 427 431 . . 428 431 + CviJI RGCY 429 429 . . 428 431 + HaeIII GGCC 429 429 . . 429 433 - BceAI ACGGC 414 416 . . 440 450 + FalI AAGNNNNNCTT 431 426 463 458 442 445 + CviJI RGCY 443 443 . . 447 452 + Hpy178III* TCNNGA 448 450 . . 440 450 - FalI AAGNNNNNCTT 463 458 431 426 451 461 + Hin4I GAYNNNNNVTC 442 437 474 469 453 456 + CviRI* TGCA 454 454 . . 451 455 - SfaNI GCATC 441 445 . . 457 460 + TaiI ACGT 460 456 . . 457 460 + SetI ASST 460 456 . . 457 460 + MaeII ACGT 457 459 . . 459 463 + Tsp45I GTSAC 458 463 . . 459 463 + MaeIII GTNAC 458 463 . . 451 461 - Hin4I GAYNNNNNVTC 474 469 442 437 462 465 + TaiI ACGT 465 461 . . 462 465 + SetI ASST 465 461 . . 462 465 + MaeII ACGT 462 464 . . 465 468 + MseI TTAA 465 467 . . 470 475 + Hpy166II GTNNAC 472 472 . . 474 478 + Tsp4CI* ACNGT 476 475 . . 480 483 + SetI ASST 483 479 . . 486 498 + PsrI GAACNNNNNNTAC 478 473 510 505 497 500 + SetI ASST 500 496 . . 502 507 + GsaI CCCAGC 506 502 . . 502 507 + BseYI CCCAGC 502 506 . . 504 509 + PvuII CAGCTG 506 506 . . 504 509 + NspBII* CMGCKG 506 506 . . 505 508 + SetI ASST 508 504 . . 505 508 + CviJI RGCY 506 506 . . 505 508 + AluI AGCT 506 506 . . 514 517 + FatI CATG 513 517 . . 514 517 + NlaIII CATG 517 513 . . 514 517 + CviAII CATG 514 516 . . 512 516 - TspDTI ATGAA 502 500 . . 517 521 + FokI GGATG 530 534 . . 517 521 + BseGI GGATG 523 521 . . 529 534 + XbaI TCTAGA 529 533 . . 529 534 + Hpy178III* TCNNGA 530 532 . . 530 533 + MaeI CTAG 530 532 . . 533 537 - SfaNI GCATC 523 527 . . 543 547 + TspDTI ATGAA 558 556 . . 552 556 + Hpy188I TCNGA 554 553 . . 540 553 - AjuI GAANNNNNNNTTGG 565 560 533 528 550 555 - Eco57MI CTGRAG 535 533 . . 550 555 - Eco57I CTGAAG 535 533 . . 560 565 + Hpy166II GTNNAC 562 562 . . 560 565 + AccI GTMKAC 561 563 . . 579 583 + TfiI GAWTC 579 582 . . 579 583 + HinfI GANTC 579 582 . . 579 589 + Hin4I GAYNNNNNVTC 570 565 602 597 587 592 + Hpy166II GTNNAC 589 589 . . 579 589 - Hin4I GAYNNNNNVTC 602 597 570 565 591 595 + Tsp4CI* ACNGT 593 592 . . 597 601 - HphI GGTGA 589 588 . . 603 607 + TspDTI ATGAA 618 616 . . 605 609 + MboII GAAGA 617 616 . . 609 614 + HindIII AAGCTT 609 613 . . 610 613 + SetI ASST 613 609 . . 610 613 + CviJI RGCY 611 611 . . 610 613 + AluI AGCT 611 611 . . 630 633 + SetI ASST 633 629 . . 633 638 + Hpy178III* TCNNGA 634 636 . . 639 642 + SetI ASST 642 638 . . 637 641 - Hin4II* CCTTC 631 630 . . 645 648 + TspEI AATT 644 648 . . 649 652 + SetI ASST 652 648 . . 655 659 - Hin4II* CCTTC 649 648 . . 669 672 + TaiI ACGT 672 668 . . 669 672 + SetI ASST 672 668 . . 669 672 + MaeII ACGT 669 671 . . 674 678 + MaeIII GTNAC 673 678 . . 683 687 - BccI CCATC 677 678 . . 696 700 + Hpy188I TCNGA 698 697 . . 720 723 + SetI ASST 723 719 . . 730 736 + TspRI CASTGNN 736 727 . . 731 735 + Tsp4CI* ACNGT 733 732 . . 735 738 + MseI TTAA 735 737 . . 737 747 + FalI AAGNNNNNCTT 728 723 760 755 739 742 + DpnI GATC 740 740 . . 739 742 + BstKTI GATC 741 739 . . 739 742 + MboI GATC 738 742 . . 741 744 + TaqI TCGA 741 743 . . 743 753 + Hin4I GAYNNNNNVTC 734 729 766 761 737 747 - FalI AAGNNNNNCTT 760 755 728 723 748 751 + CviJI RGCY 749 749 . . 748 751 + HaeIII GGCC 749 749 . . 750 753 + MnlI CCTC 760 759 . . 770 774 - TspDTI ATGAA 760 758 . . 780 783 + TaqI TCGA 780 782 . . 785 790 + Cfr10I RCCGGY 785 789 . . 782 786 - SfaNI GCATC 772 776 . . 786 789 + HpaII CCGG 786 788 . . 797 802 + Hpy166II GTNNAC 799 799 . . 801 804 + TaiI ACGT 804 800 . . 801 804 + SetI ASST 804 800 . . 801 804 + MaeII ACGT 801 803 . . 803 807 + MaeIII GTNAC 802 807 . . 806 810 + BsrI ACTGG 811 809 . . 816 820 + MaeIII GTNAC 815 820 . . 834 837 + Csp6I GTAC 834 836 . . 834 837 + RsaI GTAC 835 835 . . 842 847 + Hpy166II GTNNAC 844 844 . . 833 843 - BaeI ACNNNNGTAYC 858 853 825 820 857 866 + MslI CAYNNNNRTG 861 861 . . 857 866 + OliI CACNNNNGTG 861 861 . . 860 864 - BccI CCATC 854 855 . . 870 873 + TaqI TCGA 870 872 . . 878 883 + Hpy166II GTNNAC 880 880 . . 887 897 + FalI AAGNNNNNCTT 878 873 910 905 890 894 + HinfI GANTC 890 893 . . 890 894 - PleI GAGTC 884 885 . . 890 894 - MlyI GAGTC 884 884 . . 894 899 + StyI CCWWGG 894 898 . . 894 899 + SecI* CCNNGG 894 898 . . 887 897 - FalI AAGNNNNNCTT 910 905 878 873 911 915 + Tsp45I GTSAC 910 915 . . 911 915 + MaeIII GTNAC 910 915 . . 915 918 + MaeI CTAG 915 917 . . 930 934 - MboII GAAGA 922 921 . . 929 934 - BbvII* GAAGAC 922 926 . . 941 945 + HphI GGTGA 953 952 . . 942 947 + Hpy166II GTNNAC 944 944 . . 949 952 + SetI ASST 952 948 . . 954 957 + CviJI RGCY 955 955 . . 1002 1007 + Eco57MI CTGRAG 1023 1021 . . 1002 1007 + Eco57I CTGAAG 1023 1021 . . 1004 1008 + MboII GAAGA 1016 1015 . . 1009 1012 + TaiI ACGT 1012 1008 . . 1009 1012 + SetI ASST 1012 1008 . . 1009 1012 + MaeII ACGT 1009 1011 . . 1011 1015 + Tsp45I GTSAC 1010 1015 . . 1011 1015 + MaeIII GTNAC 1010 1015 . . 1017 1021 + MboII GAAGA 1029 1028 . . 1020 1024 + MboII GAAGA 1032 1031 . . 1023 1028 + SacI GAGCTC 1027 1023 . . 1023 1028 + Ecl136II GAGCTC 1025 1025 . . 1023 1028 + SduI GDGCHC 1027 1023 . . 1023 1028 + HgiJII* GRGCYC 1027 1023 . . 1023 1028 + HgiAI* GWGCWC 1027 1023 . . 1024 1027 + SetI ASST 1027 1023 . . 1024 1027 + CviJI RGCY 1025 1025 . . 1024 1027 + AluI AGCT 1025 1025 . . 1020 1025 - Ksp632I* CTCTTC 1015 1018 . . 1020 1026 - SapI GCTCTTC 1015 1018 . . 1033 1036 + SetI ASST 1036 1032 . . 1037 1042 + PsiI TTATAA 1039 1039 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 481 561 # # AjuI: # [GAANNNNNNNTTGG] # 32 477 533 # # AluI: # [AGCT] # 17 105 210 296 414 # # AsuI*: # [GGNCC] # 10 346 686 # # AvaII: # [GGWCC] # 10 346 686 # # BaeI: # [ACNNNNGTAYC] # 33 184 825 # # BbvI: # [GCAGC] # 305 737 # # BbvII*: # [GAAGAC] # 120 922 # # BccI: # [CCATC] # 177 188 322 355 # # BceAI: # [ACGGC] # 28 414 600 # # BetI*: # [WCCGGW] # 326 716 # # BisI: # [GCNGC] # 294 748 # # BlsI: # [GCNGC] # 295 747 # # BmgT120I: # [GGNCC] # 11 346 685 # # BseBI: # [CCWGG] # 149 893 # # BseGI: # [GGATG] # 58 86 379 519 # # BseYI: # [CCCAGC] # 502 540 # # BsiYI*: # [CCNNNNNNNGG] # 353 689 # # Bsp1407I: # [TGTACA] # 156 886 # # BsrI: # [ACTGG] # 118 193 231 500 # # BssKI: # [CCNGG] # 147 895 # # BstKTI: # [GATC] # 144 301 597 # # BstXI: # [CCANNNNNNTGG] # 306 736 # # Cfr10I: # [RCCGGY] # 257 785 # # CfrI: # [YGGCCR] # 427 615 # # Csp6I: # [GTAC] # 108 157 208 569 # # CspCI: # [CAANNNNNGTGG] # 35 348 659 # # CviAII: # [CATG] # 514 528 # # CviJI: # [RGCY] # 4 14 17 63 70 105 # 133 138 206 292 # # CviRI*: # [TGCA] # 454 588 # # DdeI: # [CTNAG] # 398 644 # # DpnI: # [GATC] # 143 302 597 # # Eam1105I: # [GACNNNNNGTC] # 322 720 # # Ecl136II: # [GAGCTC] # 17 1025 # # Eco57I: # [CTGAAG] # 19 488 535 # # Eco57MI: # [CTGRAG] # 19 488 535 # # EcoP15I: # [CAGCAG] # 323 719 # # EcoRII: # [CCWGG] # 147 895 # # FalI: # [AAGNNNNNCTT] # 0 0 0 0 0 0 # 32 32 32 118 132 265 # 431 # # FatI: # [CATG] # 513 529 # # FokI: # [GGATG] # 65 66 399 512 # # GsaI: # [CCCAGC] # 506 536 # # HaeIII: # [GGCC] # 293 320 429 # # HgiAI*: # [GWGCWC] # 15 194 833 # # HgiJII*: # [GRGCYC] # 15 1027 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 0 0 # 32 32 32 32 96 102 # 132 276 308 # # Hin4II*: # [CCTTC] # 18 393 631 # # HindIII: # [AAGCTT] # 433 609 # # HinfI: # [GANTC] # 84 152 205 290 311 # # HpaII: # [CCGG] # 256 327 459 # # HphI: # [GGTGA] # 89 364 589 # # Hpy166II: # [GTNNAC] # 27 36 45 64 90 98 # 113 210 359 # # Hpy178III*: # [TCNNGA] # 82 104 408 448 # # Hpy188I: # [TCNGA] # 144 344 554 # # Ksp632I*: # [CTCTTC] # 27 1015 # # MaeI: # [CTAG] # 5 127 385 525 # # MaeII: # [ACGT] # 5 33 132 207 208 457 # # MaeIII: # [GTNAC] # 13 32 95 100 126 129 # 215 332 # # MboI: # [GATC] # 141 304 597 # # MboII: # [GAAGA] # 3 10 13 70 94 142 # 305 405 # # McrI*: # [CGRYCG] # 430 612 # # MlyI: # [GAGTC] # 93 158 791 # # MnlI: # [CCTC] # 282 760 # # MseI: # [TTAA] # 44 55 89 270 277 307 # # MslI: # [CAYNNNNRTG] # 181 861 # # NlaIII: # [CATG] # 517 525 # # NspBII*: # [CMGCKG] # 506 536 # # OliI: # [CACNNNNGTG] # 181 861 # # PflMI: # [CCANNNNNTGG] # 353 689 # # PleI: # [GAGTC] # 92 158 792 # # PsiI: # [TTATAA] # 3 40 999 # # PsrI: # [GAACNNNNNNTAC] # 32 478 532 # # PvuII: # [CAGCTG] # 506 536 # # RsaI: # [GTAC] # 108 158 207 569 # # SacI: # [GAGCTC] # 15 1027 # # SapI: # [GCTCTTC] # 27 1015 # # ScrFI: # [CCNGG] # 149 893 # # SduI: # [GDGCHC] # 15 194 833 # # SecI*: # [CCNNGG] # 148 148 746 # # SetI: # [ASST] # 5 6 8 9 9 10 # 15 17 18 20 20 51 # 60 81 105 148 162 298 # # SfaNI: # [GCATC] # 82 155 249 270 286 # # SfeI*: # [CTRYAG] # 25 1017 # # SspI: # [AATATT] # 164 878 # # StyI: # [CCWWGG] # 148 894 # # TaiI: # [ACGT] # 5 30 132 207 208 460 # # TaqI: # [TCGA] # 39 90 172 741 # # TatI: # [WGTACW] # 156 886 # # TfiI: # [GAWTC] # 205 374 463 # # TseI: # [GCWGC] # 293 749 # # TsoI: # [TARCCA] # 106 192 744 # # Tsp45I: # [GTSAC] # 32 100 452 458 # # Tsp4CI*: # [ACNGT] # 14 117 140 309 462 # # TspDTI: # [ATGAA] # 11 56 60 84 128 142 # 279 282 # # TspEI: # [AATT] # 41 93 231 279 398 # # TspGWI: # [ACGGA] # 252 790 # # TspRI: # [CASTGNN] # 306 311 425 # # XbaI: # [TCTAGA] # 513 529 # # XmaIII*: # [CGGCCG] # 427 615 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1042 # Reported_sequences: 1 # Reported_hitcount: 228 #---------------------------------------