Restriction Fragments for TTI2/YJR136C



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The currently selected gene/sequence is :

TTI2/YJR136C on chromosome X from coordinates 678706 to 677441.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.15347.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 4 8 + BceAI ACGGC 20 22 . . 5 10 + McrI* CGRYCG 8 6 . . 5 10 + XmaIII* CGGCCG 5 9 . . 5 10 + CfrI YGGCCR 5 9 . . 6 9 + CviJI RGCY 7 7 . . 6 9 + HaeIII GGCC 7 7 . . 10 14 + MaeIII GTNAC 9 14 . . 16 21 + EcoRV GATATC 18 18 . . 22 27 + ClaI ATCGAT 23 25 . . 23 26 + TaqI TCGA 23 25 . . 26 30 + TspDTI ATGAA 41 39 . . 29 32 + TspEI AATT 28 32 . . 37 41 + TfiI GAWTC 37 40 . . 37 41 + HinfI GANTC 37 40 . . 40 43 + TaqI TCGA 40 42 . . 42 46 + PleI GAGTC 50 51 . . 42 46 + MlyI GAGTC 51 51 . . 42 46 + HinfI GANTC 42 45 . . 49 53 - MboII GAAGA 41 40 . . 59 62 + CviJI RGCY 60 60 . . 63 66 + TaqI TCGA 63 65 . . 63 68 + Hpy178III* TCNNGA 64 66 . . 74 78 + BsmAI GTCTC 79 83 . . 81 85 + MaeIII GTNAC 80 85 . . 76 81 - Eco57MI CTGRAG 61 59 . . 76 81 - GsuI CTGGAG 61 59 . . 78 82 - BsrI ACTGG 78 76 . . 90 94 + MboII GAAGA 102 101 . . 90 95 + BbvII* GAAGAC 97 101 . . 95 103 + AlwNI CAGNNNCTG 100 97 . . 100 104 + BsrI ACTGG 105 103 . . 103 107 + FokI GGATG 116 120 . . 103 107 + BseGI GGATG 109 107 . . 108 111 + CviRI* TGCA 109 109 . . 111 114 + TspEI AATT 110 114 . . 115 119 + Tsp4CI* ACNGT 117 116 . . 121 125 + Hpy188I TCNGA 123 122 . . 128 131 + SetI ASST 131 127 . . 127 130 - MnlI CCTC 120 119 . . 130 135 + Hpy166II GTNNAC 132 132 . . 130 135 + HindII GTYRAC 132 132 . . 134 137 + SetI ASST 137 133 . . 142 146 + TfiI GAWTC 142 145 . . 142 146 + HinfI GANTC 142 145 . . 168 174 + PfoI TCCNGGA 168 173 . . 169 173 + BssKI CCNGG 168 173 . . 169 173 + BseBI CCWGG 170 171 . . 169 173 + ScrFI CCNGG 170 171 . . 169 173 + EcoRII CCWGG 168 173 . . 167 172 - Eco57MI CTGRAG 152 150 . . 167 172 - GsuI CTGGAG 152 150 . . 175 180 + Hpy166II GTNNAC 177 177 . . 175 180 + AccI GTMKAC 176 178 . . 203 206 + CviRI* TGCA 204 204 . . 209 214 + SfeI* CTRYAG 209 213 . . 230 234 + MaeIII GTNAC 229 234 . . 239 244 + Hpy166II GTNNAC 241 241 . . 244 247 + FatI CATG 243 247 . . 244 247 + NlaIII CATG 247 243 . . 244 247 + CviAII CATG 244 246 . . 252 255 + TspEI AATT 251 255 . . 260 264 - HgaI GACGC 249 254 . . 272 276 + DdeI CTNAG 272 275 . . 276 280 + MaeIII GTNAC 275 280 . . 281 284 + CviJI RGCY 282 282 . . 291 296 + AhaIII* TTTAAA 293 293 . . 292 295 + MseI TTAA 292 294 . . 310 315 + AsuII TTCGAA 311 313 . . 311 314 + TaqI TCGA 311 313 . . 327 330 + TaiI ACGT 330 326 . . 327 330 + SetI ASST 330 326 . . 327 330 + MaeII ACGT 327 329 . . 337 342 + Eco31I GGTCTC 343 347 . . 338 342 + BsmAI GTCTC 343 347 . . 346 356 + BsiYI* CCNNNNNNNGG 352 349 . . 370 374 + MboII GAAGA 382 381 . . 377 380 + SetI ASST 380 376 . . 378 388 + EcoNI CCTNNNNNAGG 382 383 . . 378 388 + BsiYI* CCNNNNNNNGG 384 381 . . 383 386 + CviRI* TGCA 384 384 . . 384 389 + Cac8I GCNNGC 386 386 . . 406 409 + CviJI RGCY 407 407 . . 407 417 + MwoI GCNNNNNNNGC 413 410 . . 416 421 - BsmI GAATGC 416 414 . . 420 424 - TspGWI ACGGA 410 408 . . 430 435 + SnaBI TACGTA 432 432 . . 430 435 + BsaAI YACGTR 432 432 . . 431 434 + TaiI ACGT 434 430 . . 431 434 + SetI ASST 434 430 . . 431 434 + MaeII ACGT 431 433 . . 435 438 + TspEI AATT 434 438 . . 441 445 + MaeIII GTNAC 440 445 . . 458 462 - BsmAI GTCTC 452 456 . . 471 475 + TfiI GAWTC 471 474 . . 471 475 + HinfI GANTC 471 474 . . 489 495 + PfoI TCCNGGA 489 494 . . 490 494 + BssKI CCNGG 489 494 . . 490 494 + BseBI CCWGG 491 492 . . 490 494 + ScrFI CCNGG 491 492 . . 490 494 + EcoRII CCWGG 489 494 . . 494 499 + EcoRI GAATTC 494 498 . . 494 499 + ApoI RAATTY 494 498 . . 495 498 + TspEI AATT 494 498 . . 508 511 + MaeI CTAG 508 510 . . 519 525 + TspRI CASTGNN 525 516 . . 530 534 + MboII GAAGA 542 541 . . 534 537 + TspEI AATT 533 537 . . 536 539 + MseI TTAA 536 538 . . 559 564 + SfeI* CTRYAG 559 563 . . 564 568 + HgaI GACGC 573 578 . . 571 574 + MseI TTAA 571 573 . . 580 583 + SetI ASST 583 579 . . 586 590 + TspDTI ATGAA 601 599 . . 601 605 + HinfI GANTC 601 604 . . 601 605 - PleI GAGTC 595 596 . . 601 605 - MlyI GAGTC 595 595 . . 615 619 + Tsp4CI* ACNGT 617 616 . . 611 616 - BciVI GTATCC 605 604 . . 623 626 + MaeI CTAG 623 625 . . 625 630 + TatI WGTACW 625 629 . . 625 630 + ScaI AGTACT 627 627 . . 626 629 + Csp6I GTAC 626 628 . . 626 629 + RsaI GTAC 627 627 . . 628 632 + BsrI ACTGG 633 631 . . 641 647 + TspRI CASTGNN 647 638 . . 642 646 + Tsp4CI* ACNGT 644 643 . . 646 651 + AsuII TTCGAA 647 649 . . 647 650 + TaqI TCGA 647 649 . . 654 658 + HgaI GACGC 663 668 . . 682 685 + MnlI CCTC 692 691 . . 686 689 + MnlI CCTC 696 695 . . 701 705 + TspDTI ATGAA 716 714 . . 710 715 + TsoI TARCCA 726 724 . . 711 714 + CviJI RGCY 712 712 . . 713 723 + BsiYI* CCNNNNNNNGG 719 716 . . 714 719 + NdeI CATATG 715 717 . . 720 723 - AciI CCGC 720 722 . . 720 724 - FauI CCCGC 713 715 . . 741 745 + Tsp4CI* ACNGT 743 742 . . 738 748 - BaeI ACNNNNGTAYC 763 758 730 725 760 765 + ApoI RAATTY 760 764 . . 761 764 + TspEI AATT 760 764 . . 772 775 + TspEI AATT 771 775 . . 794 797 + SetI ASST 797 793 . . 795 799 + BssKI CCNGG 794 799 . . 795 799 + BseBI CCWGG 796 797 . . 795 799 + ScrFI CCNGG 796 797 . . 795 799 + EcoRII CCWGG 794 799 . . 792 796 - HphI GGTGA 784 783 . . 807 810 + FatI CATG 806 810 . . 807 810 + NlaIII CATG 810 806 . . 807 810 + CviAII CATG 807 809 . . 805 809 - TspDTI ATGAA 795 793 . . 811 815 + Hpy188I TCNGA 813 812 . . 824 827 + CviRI* TGCA 825 825 . . 834 837 + TspEI AATT 833 837 . . 838 843 + StyI CCWWGG 838 842 . . 838 843 + SecI* CCNNGG 838 842 . . 838 843 + AvrII CCTAGG 838 842 . . 839 842 + MaeI CTAG 839 841 . . 843 846 + CviJI RGCY 844 844 . . 844 854 + MwoI GCNNNNNNNGC 850 847 . . 844 849 + Cac8I GCNNGC 846 846 . . 844 849 + BmtI GCTAGC 848 844 . . 844 849 + NheI GCTAGC 844 848 . . 845 848 + MaeI CTAG 845 847 . . 847 850 + CviJI RGCY 848 848 . . 852 855 + CviRI* TGCA 853 853 . . 861 866 + ApoI RAATTY 861 865 . . 862 865 + TspEI AATT 861 865 . . 868 871 + TaiI ACGT 871 867 . . 868 871 + SetI ASST 871 867 . . 868 871 + MaeII ACGT 868 870 . . 877 888 + AlfI GCANNNNNNTGC 866 864 900 898 878 887 + MslI CAYNNNNRTG 882 882 . . 883 888 + BsmI GAATGC 889 887 . . 886 889 + CviRI* TGCA 887 887 . . 877 888 - AlfI GCANNNNNNTGC 900 898 866 864 892 896 + TspDTI ATGAA 907 905 . . 901 905 + DdeI CTNAG 901 904 . . 901 905 - BspCNI CTCAG 893 891 . . 901 905 - BseMII CTCAG 892 890 . . 903 907 - BsmAI GTCTC 897 901 . . 916 920 - Hin4II* CCTTC 910 909 . . 927 932 + TatI WGTACW 927 931 . . 927 932 + Bsp1407I TGTACA 927 931 . . 928 931 + Csp6I GTAC 928 930 . . 928 931 + RsaI GTAC 929 929 . . 935 940 + Hpy178III* TCNNGA 936 938 . . 937 945 + AlwNI CAGNNNCTG 942 939 . . 934 939 - Eco57MI CTGRAG 919 917 . . 934 939 - GsuI CTGGAG 919 917 . . 941 944 + CviJI RGCY 942 942 . . 939 942 - MnlI CCTC 932 931 . . 957 960 + SetI ASST 960 956 . . 958 962 + HphI GGTGA 970 969 . . 970 973 + Csp6I GTAC 970 972 . . 970 973 + RsaI GTAC 971 971 . . 987 998 + TstI CACNNNNNNTCC 978 973 1010 1005 997 1010 - AjuI GAANNNNNNNTTGG 1022 1017 990 985 1022 1028 + TspRI CASTGNN 1028 1019 . . 1030 1035 + TatI WGTACW 1030 1034 . . 1030 1035 + ScaI AGTACT 1032 1032 . . 1031 1034 + Csp6I GTAC 1031 1033 . . 1031 1034 + RsaI GTAC 1032 1032 . . 1043 1046 + SetI ASST 1046 1042 . . 1055 1059 + TspDTI ATGAA 1070 1068 . . 1068 1071 + CviJI RGCY 1069 1069 . . 1076 1080 + TfiI GAWTC 1076 1079 . . 1076 1080 + HinfI GANTC 1076 1079 . . 1079 1082 + TaqI TCGA 1079 1081 . . 1081 1091 + Hin4I GAYNNNNNVTC 1072 1067 1104 1099 1081 1091 - Hin4I GAYNNNNNVTC 1104 1099 1072 1067 1092 1097 + VspI ATTAAT 1093 1095 . . 1093 1096 + MseI TTAA 1093 1095 . . 1095 1098 + TspEI AATT 1094 1098 . . 1099 1102 + MaeI CTAG 1099 1101 . . 1102 1106 + MaeIII GTNAC 1101 1106 . . 1122 1133 + AlfI GCANNNNNNTGC 1111 1109 1145 1143 1128 1132 + FokI GGATG 1141 1145 . . 1128 1132 + BseGI GGATG 1134 1132 . . 1126 1129 - MnlI CCTC 1119 1118 . . 1129 1133 - SfaNI GCATC 1119 1123 . . 1122 1133 - AlfI GCANNNNNNTGC 1145 1143 1111 1109 1141 1145 + TfiI GAWTC 1141 1144 . . 1141 1145 + HinfI GANTC 1141 1144 . . 1174 1177 + SetI ASST 1177 1173 . . 1174 1177 + CviJI RGCY 1175 1175 . . 1174 1177 + AluI AGCT 1175 1175 . . 1186 1189 + TspEI AATT 1185 1189 . . 1190 1194 + Tsp45I GTSAC 1189 1194 . . 1190 1194 + MaeIII GTNAC 1189 1194 . . 1196 1199 + Hin6I GCGC 1196 1198 . . 1196 1199 + GlaI GCGC 1197 1197 . . 1196 1199 + HhaI GCGC 1198 1196 . . 1204 1208 + HphI GGTGA 1216 1215 . . 1205 1210 + Hpy166II GTNNAC 1207 1207 . . 1202 1207 - TaqII CACCCA 1192 1190 . . 1209 1213 + Tsp4CI* ACNGT 1211 1210 . . 1216 1219 + TaqI TCGA 1216 1218 . . 1218 1222 + HinfI GANTC 1218 1221 . . 1218 1222 - PleI GAGTC 1212 1213 . . 1218 1222 - MlyI GAGTC 1212 1212 . . 1226 1230 + DdeI CTNAG 1226 1229 . . 1234 1239 + NspBII* CMGCKG 1236 1236 . . 1234 1239 + SacII CCGCGG 1237 1235 . . 1234 1239 + DsaI* CCRYGG 1234 1238 . . 1234 1239 + SecI* CCNNGG 1234 1238 . . 1234 1237 + AciI CCGC 1234 1236 . . 1235 1238 + FnuDII* CGCG 1236 1236 . . 1236 1239 - AciI CCGC 1236 1238 . . 1245 1249 + MaeIII GTNAC 1244 1249 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 176 1090 # # AciI: # [CCGC] # 2 30 514 720 # # AhaIII*: # [TTTAAA] # 293 973 # # AjuI: # [GAANNNNNNNTTGG] # 32 244 990 # # AlfI: # [GCANNNNNNTGC] # 0 0 0 0 34 34 # 121 211 866 # # AluI: # [AGCT] # 91 1175 # # AlwNI: # [CAGNNNCTG] # 100 324 842 # # ApoI: # [RAATTY] # 101 266 405 494 # # AsuII: # [TTCGAA] # 311 336 619 # # AvrII: # [CCTAGG] # 428 838 # # BaeI: # [ACNNNNGTAYC] # 33 503 730 # # BbvII*: # [GAAGAC] # 97 1169 # # BceAI: # [ACGGC] # 20 1246 # # BciVI: # [GTATCC] # 605 661 # # BmtI: # [GCTAGC] # 418 848 # # BsaAI: # [YACGTR] # 432 834 # # BseBI: # [CCWGG] # 170 305 321 470 # # BseGI: # [GGATG] # 109 132 1025 # # BseMII: # [CTCAG] # 374 892 # # BsiYI*: # [CCNNNNNNNGG] # 32 335 352 547 # # BsmAI: # [GTCTC] # 79 109 264 369 445 # # BsmI: # [GAATGC] # 377 416 473 # # Bsp1407I: # [TGTACA] # 339 927 # # BspCNI: # [CTCAG] # 373 893 # # BsrI: # [ACTGG] # 27 78 528 633 # # BssKI: # [CCNGG] # 168 305 321 472 # # Cac8I: # [GCNNGC] # 386 420 460 # # CfrI: # [YGGCCR] # 5 1261 # # ClaI: # [ATCGAT] # 23 1243 # # Csp6I: # [GTAC] # 42 61 235 302 626 # # CviAII: # [CATG] # 244 459 563 # # CviJI: # [RGCY] # 4 7 53 91 94 106 # 125 127 132 222 305 # # CviRI*: # [TGCA] # 28 34 95 109 180 379 # 441 # # DdeI: # [CTNAG] # 40 272 325 629 # # DsaI*: # [CCRYGG] # 32 1234 # # Eco31I: # [GGTCTC] # 343 923 # # Eco57MI: # [CTGRAG] # 61 91 347 767 # # EcoNI: # [CCTNNNNNAGG] # 382 884 # # EcoRI: # [GAATTC] # 494 772 # # EcoRII: # [CCWGG] # 168 305 321 472 # # EcoRV: # [GATATC] # 18 1248 # # FatI: # [CATG] # 243 460 563 # # FauI: # [CCCGC] # 553 713 # # FnuDII*: # [CGCG] # 30 1236 # # FokI: # [GGATG] # 116 125 1025 # # GlaI: # [GCGC] # 69 1197 # # GsuI: # [CTGGAG] # 61 91 347 767 # # HaeIII: # [GGCC] # 7 1259 # # HgaI: # [GACGC] # 90 249 324 603 # # HhaI: # [GCGC] # 68 1198 # # Hin4I: # [GAYNNNNNVTC] # 0 0 32 162 1072 # # Hin4II*: # [CCTTC] # 356 910 # # Hin6I: # [GCGC] # 70 1196 # # HindII: # [GTYRAC] # 132 1134 # # HinfI: # [GANTC] # 5 37 48 65 77 100 # 130 329 475 # # HphI: # [GGTGA] # 50 186 246 784 # # Hpy166II: # [GTNNAC] # 45 59 64 132 966 # # Hpy178III*: # [TCNNGA] # 64 330 872 # # Hpy188I: # [TCNGA] # 123 453 690 # # MaeI: # [CTAG] # 6 115 167 216 254 508 # # MaeII: # [ACGT] # 104 327 398 437 # # MaeIII: # [GTNAC] # 9 22 46 55 71 88 # 149 165 661 # # MboII: # [GAAGA] # 41 61 160 280 724 # # McrI*: # [CGRYCG] # 8 1258 # # MlyI: # [GAGTC] # 51 54 544 617 # # MnlI: # [CCTC] # 4 120 147 187 236 572 # # MseI: # [TTAA] # 35 173 244 292 522 # # MslI: # [CAYNNNNRTG] # 384 882 # # MwoI: # [GCNNNNNNNGC] # 413 416 437 # # NdeI: # [CATATG] # 551 715 # # NheI: # [GCTAGC] # 422 844 # # NlaIII: # [CATG] # 247 456 563 # # NspBII*: # [CMGCKG] # 30 1236 # # PfoI: # [TCCNGGA] # 168 321 777 # # PleI: # [GAGTC] # 50 54 545 617 # # RsaI: # [GTAC] # 42 61 234 302 627 # # SacII: # [CCGCGG] # 29 1237 # # ScaI: # [AGTACT] # 234 405 627 # # ScrFI: # [CCNGG] # 170 305 321 470 # # SecI*: # [CCNNGG] # 32 396 838 # # SetI: # [ASST] # 6 50 54 74 86 89 # 89 131 131 149 193 214 # # SfaNI: # [GCATC] # 147 1119 # # SfeI*: # [CTRYAG] # 209 350 707 # # SnaBI: # [TACGTA] # 432 834 # # StyI: # [CCWWGG] # 428 838 # # TaiI: # [ACGT] # 104 330 395 437 # # TaqI: # [TCGA] # 17 23 23 50 137 248 # 336 432 # # TaqII: # [CACCCA] # 74 1192 # # TatI: # [WGTACW] # 103 236 302 625 # # TfiI: # [GAWTC] # 37 65 105 125 329 605 # # TsoI: # [TARCCA] # 540 726 # # Tsp45I: # [GTSAC] # 77 1189 # # Tsp4CI*: # [ACNGT] # 27 55 99 117 468 500 # # TspDTI: # [ATGAA] # 41 79 112 115 163 196 # 560 # # TspEI: # [AATT] # 11 28 28 39 60 62 # 81 82 91 141 183 227 # 233 # # TspGWI: # [ACGGA] # 410 856 # # TspRI: # [CASTGNN] # 122 238 381 525 # # TstI: # [CACNNNNNNTCC] # 32 256 978 # # VspI: # [ATTAAT] # 173 1093 # # XmaIII*: # [CGGCCG] # 5 1261 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1266 # Reported_sequences: 1 # Reported_hitcount: 252 #---------------------------------------