Restriction Fragments for TDH2/YJR009C



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The currently selected gene/sequence is :

TDH2/YJR009C on chromosome X from coordinates 454681 to 453683.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.17747.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 17 20 + MseI TTAA 17 19 . . 20 24 + Tsp4CI* ACNGT 22 21 . . 32 36 + TfiI GAWTC 32 35 . . 32 36 + HinfI GANTC 32 35 . . 44 49 - TsoI TARCCA 34 32 . . 54 57 + TspEI AATT 53 57 . . 62 65 + CviRI* TGCA 63 63 . . 74 77 + TaiI ACGT 77 73 . . 74 77 + SetI ASST 77 73 . . 74 77 + MaeII ACGT 74 76 . . 75 79 + Hpy99I CGWCG 79 74 . . 77 80 + TaqI TCGA 77 79 . . 78 89 + BcgI CGANNNNNNTGC 67 65 101 99 97 100 + DpnI GATC 98 98 . . 97 100 + BstKTI GATC 99 97 . . 97 100 + MboI GATC 96 100 . . 97 101 - BinI* GGATC 91 92 . . 103 107 - TspDTI ATGAA 93 91 . . 113 123 + BsaXI ACNNNNNCTCC 103 100 133 130 122 125 + AciI CCGC 122 124 . . 128 133 + BspLU11I* ACATGT 128 132 . . 128 133 + NspI RCATGY 132 128 . . 128 133 + AflIII ACRYGT 128 132 . . 129 132 + FatI CATG 128 132 . . 129 132 + NlaIII CATG 132 128 . . 129 132 + CviAII CATG 129 131 . . 138 141 + Csp6I GTAC 138 140 . . 138 141 + RsaI GTAC 139 139 . . 142 146 + HinfI GANTC 142 145 . . 142 146 - PleI GAGTC 136 137 . . 142 146 - MlyI GAGTC 136 136 . . 152 156 + Tsp4CI* ACNGT 154 153 . . 166 170 + HphI GGTGA 178 177 . . 158 168 - BaeI ACNNNNGTAYC 183 178 150 145 206 210 + Tsp45I GTSAC 205 210 . . 206 210 + MaeIII GTNAC 205 210 . . 202 206 - BccI CCATC 196 197 . . 213 216 + DpnI GATC 214 214 . . 213 216 + BstKTI GATC 215 213 . . 213 216 + MboI GATC 212 216 . . 237 242 + GsaI CCCAGC 241 237 . . 237 242 + BseYI CCCAGC 237 241 . . 233 237 - BsmAI GTCTC 227 231 . . 233 238 - Eco31I GGTCTC 227 231 . . 240 243 + SetI ASST 243 239 . . 240 243 + CviJI RGCY 241 241 . . 240 243 + AluI AGCT 241 241 . . 250 255 + DsaI* CCRYGG 250 254 . . 250 255 + StyI CCWWGG 250 254 . . 250 255 + NcoI CCATGG 250 254 . . 250 255 + SecI* CCNNGG 250 254 . . 251 254 + FatI CATG 250 254 . . 251 254 + NlaIII CATG 254 250 . . 251 254 + CviAII CATG 251 253 . . 255 258 + CviJI RGCY 256 256 . . 273 278 - BtgZI GCGATG 258 262 . . 283 287 + HinfI GANTC 283 286 . . 283 287 - PleI GAGTC 277 278 . . 283 287 - MlyI GAGTC 277 277 . . 288 294 + TspRI CASTGNN 294 285 . . 289 293 + BsrI ACTGG 294 292 . . 305 308 + TspEI AATT 304 308 . . 312 318 + TspRI CASTGNN 318 309 . . 312 317 - BtsI GCAGTG 311 309 . . 331 335 + HgaI GACGC 340 345 . . 337 342 + NlaIV GGNNCC 339 339 . . 337 342 + HgiCI* GGYRCC 337 341 . . 347 350 + SetI ASST 350 346 . . 345 349 - Hin4II* CCTTC 339 338 . . 357 363 + TspRI CASTGNN 363 354 . . 357 362 - BtsI GCAGTG 356 354 . . 364 368 + BccI CCATC 372 373 . . 367 371 - MboII GAAGA 359 358 . . 374 377 + AciI CCGC 374 376 . . 390 393 + FatI CATG 389 393 . . 390 393 + NlaIII CATG 393 389 . . 390 393 + CviAII CATG 390 392 . . 392 397 - TaqII CACCCA 382 380 . . 397 402 + Hpy166II GTNNAC 399 399 . . 397 402 + HpaI GTTAAC 399 399 . . 397 402 + HindII GTYRAC 399 399 . . 398 401 + MseI TTAA 398 400 . . 403 407 + MboII GAAGA 415 414 . . 418 422 + Hpy188I TCNGA 420 419 . . 426 430 + MboII GAAGA 438 437 . . 436 441 + MmeI TCCRAC 461 459 . . 449 452 + Csp6I GTAC 449 451 . . 449 452 + RsaI GTAC 450 450 . . 452 466 + XcmI CCANNNNNNNNNTGG 459 458 . . 455 466 + BstXI CCANNNNNNTGG 462 458 . . 458 462 + Tsp4CI* ACNGT 460 459 . . 465 468 + CviJI RGCY 466 466 . . 465 470 + NlaIV GGNNCC 467 467 . . 466 476 + MwoI GCNNNNNNNGC 472 469 . . 473 478 + BalI TGGCCA 475 475 . . 473 478 + CfrI YGGCCR 473 477 . . 474 477 + CviJI RGCY 475 475 . . 474 477 + HaeIII GGCC 475 475 . . 476 481 + StyI CCWWGG 476 480 . . 476 481 + SecI* CCNNGG 476 480 . . 479 482 + SetI ASST 482 478 . . 490 494 - SfaNI GCATC 480 484 . . 505 509 + MboII GAAGA 517 516 . . 510 513 + SetI ASST 513 509 . . 508 512 - Hin4II* CCTTC 502 501 . . 522 528 + TspRI CASTGNN 528 519 . . 523 527 + Tsp4CI* ACNGT 525 524 . . 526 531 + Hpy166II GTNNAC 528 528 . . 534 537 + FatI CATG 533 537 . . 534 537 + NlaIII CATG 537 533 . . 534 537 + CviAII CATG 534 536 . . 539 542 + AciI CCGC 539 541 . . 543 548 + TaqII CACCCA 559 557 . . 553 557 + Tsp4CI* ACNGT 555 554 . . 556 561 + Hpy166II GTNNAC 558 558 . . 556 561 + HindII GTYRAC 558 558 . . 559 569 + Hin4I GAYNNNNNVTC 550 545 582 577 560 564 + Tsp4CI* ACNGT 562 561 . . 562 566 + BmgT120I GGNCC 563 564 . . 562 566 + AvaII GGWCC 562 565 . . 562 566 + AsuI* GGNCC 562 565 . . 565 569 + BccI CCATC 573 574 . . 559 569 - Hin4I GAYNNNNNVTC 582 577 550 545 566 570 - FokI GGATG 552 556 . . 566 570 - BseGI GGATG 565 563 . . 578 582 + BsrI ACTGG 583 581 . . 579 584 + Eco57MI CTGRAG 600 598 . . 579 584 + GsuI CTGGAG 600 598 . . 585 588 + SetI ASST 588 584 . . 584 587 - MnlI CCTC 577 576 . . 581 591 - BsaXI ACNNNNNCTCC 603 600 573 570 593 602 + XmnI GAANNNNTTC 597 597 . . 596 599 + AciI CCGC 596 598 . . 601 606 + BetI* WCCGGW 601 605 . . 602 605 + HpaII CCGG 602 604 . . 590 602 - BarI GAAGNNNNNNTAC 614 609 582 577 605 609 + MaeIII GTNAC 604 609 . . 612 616 - FokI GGATG 598 602 . . 612 616 - BseGI GGATG 611 609 . . 616 620 + BccI CCATC 624 625 . . 620 623 + MnlI CCTC 630 629 . . 617 621 - FokI GGATG 603 607 . . 617 621 - BseGI GGATG 616 614 . . 625 630 + Cfr10I RCCGGY 625 629 . . 625 630 + BetI* WCCGGW 625 629 . . 625 630 + AgeI ACCGGT 625 629 . . 626 629 + HpaII CCGG 626 628 . . 631 641 + MwoI GCNNNNNNNGC 637 634 . . 631 635 + BlsI GCNGC 633 632 . . 631 635 + BisI GCNGC 632 633 . . 631 635 + TseI GCWGC 631 634 . . 635 639 + DdeI CTNAG 635 638 . . 631 635 - BbvI GCAGC 618 622 . . 639 642 + CviJI RGCY 640 640 . . 634 640 - Bpu10I CCTNAGC 635 638 . . 650 653 + SetI ASST 653 649 . . 657 667 + MwoI GCNNNNNNNGC 663 660 . . 662 665 + TspEI AATT 661 665 . . 665 668 + CviRI* TGCA 666 666 . . 669 672 + SetI ASST 672 668 . . 675 680 + Hpy166II GTNNAC 677 677 . . 675 680 + HindII GTYRAC 677 677 . . 679 684 + Cfr10I RCCGGY 679 683 . . 679 684 + BetI* WCCGGW 679 683 . . 679 684 + AgeI ACCGGT 679 683 . . 680 683 + HpaII CCGG 680 682 . . 687 690 + CviJI RGCY 688 688 . . 692 696 + Hpy188I TCNGA 694 693 . . 695 699 + PleI GAGTC 703 704 . . 695 699 + MlyI GAGTC 704 704 . . 695 699 + HinfI GANTC 695 698 . . 697 701 - BslFI GGGAC 682 686 . . 703 707 + Tsp4CI* ACNGT 705 704 . . 705 709 + Hpy99I CGWCG 709 704 . . 707 710 + TaqI TCGA 707 709 . . 715 719 - TspGWI ACGGA 705 703 . . 721 726 + Hpy166II GTNNAC 723 723 . . 721 726 + HindII GTYRAC 723 723 . . 724 735 + DrdI GACNNNNNNGTC 730 728 . . 730 734 + Tsp4CI* ACNGT 732 731 . . 754 757 + SetI ASST 757 753 . . 761 765 + TspDTI ATGAA 776 774 . . 767 772 + Hpy178III* TCNNGA 768 770 . . 773 776 + SetI ASST 776 772 . . 771 775 - Hin4II* CCTTC 765 764 . . 783 786 + CviJI RGCY 784 784 . . 784 788 + BlsI GCNGC 786 785 . . 784 788 + BisI GCNGC 785 786 . . 784 788 + TseI GCWGC 784 787 . . 787 791 + TauI GCSGC 790 787 . . 787 791 + BlsI GCNGC 789 788 . . 787 791 + BisI GCNGC 788 789 . . 788 793 + NspBII* CMGCKG 790 790 . . 784 788 - BbvI GCAGC 771 775 . . 788 791 + AciI CCGC 788 790 . . 791 796 + Eco57MI CTGRAG 812 810 . . 791 796 + Eco57I CTGAAG 812 810 . . 795 798 + SetI ASST 798 794 . . 793 797 - Hin4II* CCTTC 787 786 . . 804 817 + AjuI GAANNNNNNNTTGG 796 791 828 823 805 815 + FalI AAGNNNNNCTT 796 791 828 823 804 808 - Hin4II* CCTTC 798 797 . . 805 815 - FalI AAGNNNNNCTT 828 823 796 791 818 822 + MaeIII GTNAC 817 822 . . 822 828 + TspRI CASTGNN 828 819 . . 824 829 + Eco57MI CTGRAG 845 843 . . 824 829 + Eco57I CTGAAG 845 843 . . 826 830 + MboII GAAGA 838 837 . . 826 831 + BbvII* GAAGAC 833 837 . . 829 833 + HgaI GACGC 838 843 . . 829 840 + DrdI GACNNNNNNGTC 835 833 . . 838 842 + BsmAI GTCTC 843 847 . . 842 845 + MnlI CCTC 852 851 . . 844 848 + Hpy188I TCNGA 846 845 . . 840 845 - BseRI GAGGAG 831 829 . . 856 860 + HphI GGTGA 868 867 . . 857 861 + Tsp45I GTSAC 856 861 . . 857 861 + MaeIII GTNAC 856 861 . . 854 859 - TaqII CACCCA 844 842 . . 859 863 + HinfI GANTC 859 862 . . 859 869 + Hin4I GAYNNNNNVTC 850 845 882 877 859 863 - PleI GAGTC 853 854 . . 859 863 - MlyI GAGTC 853 853 . . 867 872 + Ksp632I* CTCTTC 873 876 . . 868 872 - MboII GAAGA 860 859 . . 872 876 + BccI CCATC 880 881 . . 878 881 + TaqI TCGA 878 880 . . 875 879 - MboII GAAGA 867 866 . . 883 887 + BlsI GCNGC 885 884 . . 883 887 + BisI GCNGC 884 885 . . 883 887 + TseI GCWGC 883 886 . . 880 884 - SfaNI GCATC 870 874 . . 886 890 + TauI GCSGC 889 886 . . 886 890 + BlsI GCNGC 888 887 . . 886 890 + BisI GCNGC 887 888 . . 887 892 + NspBII* CMGCKG 889 889 . . 883 887 - BbvI GCAGC 870 874 . . 887 890 + AciI CCGC 887 889 . . 893 898 + BciVI GTATCC 904 903 . . 898 903 + MfeI CAATTG 898 902 . . 899 902 + TspEI AATT 898 902 . . 903 907 + BsmAI GTCTC 908 912 . . 929 933 + BssKI CCNGG 928 933 . . 929 933 + BseBI CCWGG 930 931 . . 929 933 + ScrFI CCNGG 930 931 . . 929 933 + EcoRII CCWGG 928 933 . . 933 936 + Csp6I GTAC 933 935 . . 933 936 + RsaI GTAC 934 934 . . 947 951 + Tsp4CI* ACNGT 949 948 . . 950 954 + MaeIII GTNAC 949 954 . . 967 972 + SalI GTCGAC 967 971 . . 967 972 + Hpy166II GTNNAC 969 969 . . 967 972 + HindII GTYRAC 969 969 . . 967 972 + AccI GTMKAC 968 970 . . 968 971 + TaqI TCGA 968 970 . . 981 986 + AflIII ACRYGT 981 985 . . 983 986 + TaiI ACGT 986 982 . . 983 986 + SetI ASST 986 982 . . 983 986 + MaeII ACGT 983 985 . . 989 994 + StyI CCWWGG 989 993 . . 989 994 + SecI* CCNNGG 989 993 . . 993 996 + CviJI RGCY 994 994 . . 996 999 + MseI TTAA 996 998 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 31 968 # # AciI: # [CCGC] # 57 99 112 122 165 192 # 252 # # AflIII: # [ACRYGT] # 18 128 853 # # AgeI: # [ACCGGT] # 54 320 625 # # AjuI: # [GAANNNNNNNTTGG] # 32 171 796 # # AluI: # [AGCT] # 241 758 # # AsuI*: # [GGNCC] # 437 562 # # AvaII: # [GGWCC] # 437 562 # # BaeI: # [ACNNNNGTAYC] # 33 150 816 # # BalI: # [TGGCCA] # 475 524 # # BarI: # [GAAGNNNNNNTAC] # 32 385 582 # # BbvI: # [GCAGC] # 99 129 153 618 # # BbvII*: # [GAAGAC] # 166 833 # # BccI: # [CCATC] # 51 119 176 196 201 256 # # BcgI: # [CGANNNNNNTGC] # 34 67 898 # # BciVI: # [GTATCC] # 95 904 # # BetI*: # [WCCGGW] # 24 54 320 601 # # BinI*: # [GGATC] # 91 908 # # BisI: # [GCNGC] # 3 3 96 112 153 632 # # BlsI: # [GCNGC] # 3 3 96 111 153 633 # # BmgT120I: # [GGNCC] # 436 563 # # Bpu10I: # [CCTNAGC] # 364 635 # # BsaXI: # [ACNNNNNCTCC] # 30 30 103 396 440 # # BseBI: # [CCWGG] # 69 930 # # BseGI: # [GGATG] # 5 46 383 565 # # BseRI: # [GAGGAG] # 168 831 # # BseYI: # [CCCAGC] # 237 762 # # BslFI: # [GGGAC] # 317 682 # # BsmAI: # [GTCTC] # 65 91 227 616 # # BspLU11I*: # [ACATGT] # 128 871 # # BsrI: # [ACTGG] # 289 294 416 # # BssKI: # [CCNGG] # 71 928 # # BstKTI: # [GATC] # 99 116 784 # # BstXI: # [CCANNNNNNTGG] # 462 537 # # BtgZI: # [GCGATG] # 258 741 # # BtsI: # [GCAGTG] # 45 311 643 # # Cfr10I: # [RCCGGY] # 54 320 625 # # CfrI: # [YGGCCR] # 473 526 # # Csp6I: # [GTAC] # 66 138 311 484 # # CviAII: # [CATG] # 122 129 139 144 465 # # CviJI: # [RGCY] # 5 9 15 48 96 165 # 210 210 241 # # CviRI*: # [TGCA] # 63 333 603 # # DdeI: # [CTNAG] # 364 635 # # DpnI: # [GATC] # 98 116 785 # # DrdI: # [GACNNNNNNGTC] # 105 164 730 # # DsaI*: # [CCRYGG] # 250 749 # # Eco31I: # [GGTCTC] # 227 772 # # Eco57I: # [CTGAAG] # 33 154 812 # # Eco57MI: # [CTGRAG] # 33 154 212 600 # # EcoRII: # [CCWGG] # 71 928 # # FalI: # [AAGNNNNNCTT] # 0 0 32 171 796 # # FatI: # [CATG] # 122 128 139 144 466 # # FokI: # [GGATG] # 5 46 396 552 # # GsaI: # [CCCAGC] # 241 758 # # GsuI: # [CTGGAG] # 399 600 # # HaeIII: # [GGCC] # 475 524 # # HgaI: # [GACGC] # 161 340 498 # # HgiCI*: # [GGYRCC] # 337 662 # # Hin4I: # [GAYNNNNNVTC] # 0 0 32 32 117 268 # 550 # # Hin4II*: # [CCTTC] # 11 22 163 201 263 339 # # HindII: # [GTYRAC] # 30 46 119 159 246 399 # # HinfI: # [GANTC] # 32 110 140 141 164 412 # # HpaI: # [GTTAAC] # 399 600 # # HpaII: # [CCGG] # 24 54 319 602 # # HphI: # [GGTGA] # 131 178 690 # # Hpy166II: # [GTNNAC] # 30 30 46 119 129 246 # 399 # # Hpy178III*: # [TCNNGA] # 231 768 # # Hpy188I: # [TCNGA] # 152 153 274 420 # # Hpy99I: # [CGWCG] # 79 290 630 # # Ksp632I*: # [CTCTTC] # 126 873 # # MaeII: # [ACGT] # 16 74 909 # # MaeIII: # [GTNAC] # 39 50 93 205 213 399 # # MboI: # [GATC] # 96 116 787 # # MboII: # [GAAGA] # 7 22 23 56 79 132 # 321 359 # # MfeI: # [CAATTG] # 101 898 # # MlyI: # [GAGTC] # 136 141 146 149 427 # # MmeI: # [TCCRAC] # 461 538 # # MnlI: # [CCTC] # 53 147 222 577 # # MseI: # [TTAA] # 3 17 381 598 # # MwoI: # [GCNNNNNNNGC] # 26 165 336 472 # # NcoI: # [CCATGG] # 250 749 # # NlaIII: # [CATG] # 122 132 139 144 462 # # NlaIV: # [GGNNCC] # 128 339 532 # # NspBII*: # [CMGCKG] # 99 110 790 # # NspI: # [RCATGY] # 132 867 # # PleI: # [GAGTC] # 136 141 146 150 426 # # RsaI: # [GTAC] # 65 139 311 484 # # SalI: # [GTCGAC] # 32 967 # # ScrFI: # [CCNGG] # 69 930 # # SecI*: # [CCNNGG] # 10 226 250 513 # # SetI: # [ASST] # 13 19 19 22 31 65 # 75 77 85 107 132 166 # 188 # # SfaNI: # [GCATC] # 129 390 480 # # StyI: # [CCWWGG] # 10 226 250 513 # # TaiI: # [ACGT] # 13 77 909 # # TaqI: # [TCGA] # 31 77 90 171 630 # # TaqII: # [CACCCA] # 155 177 285 382 # # TauI: # [GCSGC] # 99 110 790 # # TfiI: # [GAWTC] # 32 967 # # TseI: # [GCWGC] # 99 116 153 631 # # TsoI: # [TARCCA] # 34 965 # # Tsp45I: # [GTSAC] # 143 205 651 # # Tsp4CI*: # [ACNGT] # 7 22 27 30 50 65 # 132 143 217 306 # # TspDTI: # [ATGAA] # 93 223 683 # # TspEI: # [AATT] # 53 101 237 251 357 # # TspGWI: # [ACGGA] # 294 705 # # TspRI: # [CASTGNN] # 24 45 165 171 294 300 # # XcmI: # [CCANNNNNNNNNTGG] # 459 540 # # XmnI: # [GAANNNNTTC] # 402 597 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 999 # Reported_sequences: 1 # Reported_hitcount: 263 #---------------------------------------