Restriction Fragments for YJLWtau4



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

YJLWtau4 on chromosome X from coordinates 421946 to 422295.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.31625.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 8 11 + FatI CATG 7 11 . . 8 11 + NlaIII CATG 11 7 . . 8 11 + CviAII CATG 8 10 . . 9 13 + TspDTI ATGAA 24 22 . . 11 16 + EcoRI GAATTC 11 15 . . 11 16 + ApoI RAATTY 11 15 . . 12 15 + TspEI AATT 11 15 . . 16 21 + MfeI CAATTG 16 20 . . 17 20 + TspEI AATT 16 20 . . 22 26 + MaeIII GTNAC 21 26 . . 27 30 + TspEI AATT 26 30 . . 31 34 + TaqI TCGA 31 33 . . 33 37 - SfaNI GCATC 23 27 . . 50 53 + MaeI CTAG 50 52 . . 53 58 + Hpy166II GTNNAC 55 55 . . 53 58 + BssNAI GTATAC 55 55 . . 53 58 + AccI GTMKAC 54 56 . . 61 70 + MslI CAYNNNNRTG 65 65 . . 65 70 + NdeI CATATG 66 68 . . 80 83 + Csp6I GTAC 80 82 . . 80 83 + RsaI GTAC 81 81 . . 82 85 + SetI ASST 85 81 . . 92 96 - Hin4II* CCTTC 86 85 . . 103 108 + Hpy178III* TCNNGA 104 106 . . 107 116 + XmnI GAANNNNTTC 111 111 . . 115 119 + Hpy188I TCNGA 117 116 . . 119 123 + Tsp4CI* ACNGT 121 120 . . 122 125 + Csp6I GTAC 122 124 . . 122 125 + RsaI GTAC 123 123 . . 134 137 + TspEI AATT 133 137 . . 136 139 + MseI TTAA 136 138 . . 159 162 + DpnI GATC 160 160 . . 159 162 + BstKTI GATC 161 159 . . 159 162 + MboI GATC 158 162 . . 161 165 + Hpy188I TCNGA 163 162 . . 170 179 + MslI CAYNNNNRTG 174 174 . . 188 191 + SetI ASST 191 187 . . 191 195 - MboII GAAGA 183 182 . . 190 195 - BbvII* GAAGAC 183 187 . . 199 202 + MseI TTAA 199 201 . . 217 220 + HpaII CCGG 217 219 . . 219 224 + NlaIV GGNNCC 221 221 . . 220 232 + PsrI GAACNNNNNNTAC 212 207 244 239 223 237 + XcmI CCANNNNNNNNNTGG 230 229 . . 237 241 + Tsp45I GTSAC 236 241 . . 237 241 + MaeIII GTNAC 236 241 . . 242 245 + TspEI AATT 241 245 . . 250 254 + TspDTI ATGAA 265 263 . . 292 296 + DdeI CTNAG 292 295 . . 296 300 + MboII GAAGA 308 307 . . 299 302 + DpnI GATC 300 300 . . 299 302 + BstKTI GATC 301 299 . . 299 302 + MboI GATC 298 302 . . 300 305 + ClaI ATCGAT 301 303 . . 301 304 + TaqI TCGA 301 303 . . 303 313 + Hin4I GAYNNNNNVTC 294 289 326 321 303 313 - Hin4I GAYNNNNNVTC 326 321 294 289 312 316 - MboII GAAGA 304 303 . . 317 322 + VspI ATTAAT 318 320 . . 314 318 - TspDTI ATGAA 304 302 . . 318 321 + MseI TTAA 318 320 . . 324 329 + HindIII AAGCTT 324 328 . . 325 328 + SetI ASST 328 324 . . 325 328 + CviJI RGCY 326 326 . . 325 328 + AluI AGCT 326 326 . . 334 337 + SetI ASST 337 333 . . 335 338 + MnlI CCTC 345 344 . . 340 343 + SetI ASST 343 339 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 54 296 # # AluI: # [AGCT] # 24 326 # # ApoI: # [RAATTY] # 11 339 # # BbvII*: # [GAAGAC] # 167 183 # # BssNAI: # [GTATAC] # 55 295 # # BstKTI: # [GATC] # 49 140 161 # # ClaI: # [ATCGAT] # 49 301 # # Csp6I: # [GTAC] # 42 80 228 # # CviAII: # [CATG] # 8 342 # # CviJI: # [RGCY] # 24 326 # # DdeI: # [CTNAG] # 58 292 # # DpnI: # [GATC] # 50 140 160 # # EcoRI: # [GAATTC] # 11 339 # # FatI: # [CATG] # 7 343 # # Hin4I: # [GAYNNNNNVTC] # 0 0 24 32 294 # # Hin4II*: # [CCTTC] # 86 264 # # HindIII: # [AAGCTT] # 26 324 # # HpaII: # [CCGG] # 133 217 # # Hpy166II: # [GTNNAC] # 55 295 # # Hpy178III*: # [TCNNGA] # 104 246 # # Hpy188I: # [TCNGA] # 46 117 187 # # MaeI: # [CTAG] # 50 300 # # MaeIII: # [GTNAC] # 21 114 215 # # MboI: # [GATC] # 52 140 158 # # MboII: # [GAAGA] # 4 42 121 183 # # MfeI: # [CAATTG] # 16 334 # # MnlI: # [CCTC] # 5 345 # # MseI: # [TTAA] # 32 63 119 136 # # MslI: # [CAYNNNNRTG] # 65 109 176 # # NdeI: # [CATATG] # 66 284 # # NlaIII: # [CATG] # 11 339 # # NlaIV: # [GGNNCC] # 129 221 # # PsrI: # [GAACNNNNNNTAC] # 32 106 212 # # RsaI: # [GTAC] # 42 81 227 # # SetI: # [ASST] # 6 7 9 85 106 137 # # SfaNI: # [GCATC] # 23 327 # # TaqI: # [TCGA] # 31 49 270 # # Tsp45I: # [GTSAC] # 114 236 # # Tsp4CI*: # [ACNGT] # 121 229 # # TspDTI: # [ATGAA] # 24 39 46 241 # # TspEI: # [AATT] # 5 10 11 107 108 109 # # VspI: # [ATTAAT] # 32 318 # # XcmI: # [CCANNNNNNNNNTGG] # 120 230 # # XmnI: # [GAANNNNTTC] # 111 239 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 350 # Reported_sequences: 1 # Reported_hitcount: 68 #---------------------------------------