Restriction Fragments for RFA3/YJL173C



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The currently selected gene/sequence is :

RFA3/YJL173C on chromosome X from coordinates 96529 to 96161.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.25375.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 2 7 + BalI TGGCCA 4 4 . . 2 7 + CfrI YGGCCR 2 6 . . 3 6 + CviJI RGCY 4 4 . . 3 6 + HaeIII GGCC 4 4 . . 4 9 + Cac8I GCNNGC 6 6 . . 22 27 + Hpy166II GTNNAC 24 24 . . 22 27 + HindII GTYRAC 24 24 . . 40 45 + MmeI TCCRAC 65 63 . . 44 47 + TaiI ACGT 47 43 . . 44 47 + SetI ASST 47 43 . . 44 47 + MaeII ACGT 44 46 . . 73 76 + Hin6I GCGC 73 75 . . 73 76 + GlaI GCGC 74 74 . . 73 76 + HhaI GCGC 75 73 . . 93 99 + TspRI CASTGNN 99 90 . . 97 101 + TfiI GAWTC 97 100 . . 97 101 + HinfI GANTC 97 100 . . 101 105 + DdeI CTNAG 101 104 . . 101 105 + BspCNI CTCAG 114 112 . . 101 105 + BseMII CTCAG 115 113 . . 133 136 + TaqI TCGA 133 135 . . 140 144 + BceAI ACGGC 156 158 . . 143 147 + BlsI GCNGC 145 144 . . 143 147 + BisI GCNGC 144 145 . . 143 147 + BbvI GCAGC 155 159 . . 143 147 + TseI GCWGC 143 146 . . 149 152 + SetI ASST 152 148 . . 148 151 - MnlI CCTC 141 140 . . 163 166 + TaiI ACGT 166 162 . . 163 166 + SetI ASST 166 162 . . 163 166 + MaeII ACGT 163 165 . . 172 177 + SspI AATATT 174 174 . . 187 191 + TspDTI ATGAA 202 200 . . 202 212 + BplI GAGNNNNNCTC 193 188 225 220 204 207 + DpnI GATC 205 205 . . 204 207 + BstKTI GATC 206 204 . . 204 207 + MboI GATC 203 207 . . 206 209 + TaqI TCGA 206 208 . . 208 212 + HinfI GANTC 208 211 . . 208 212 - PleI GAGTC 202 203 . . 208 212 - MlyI GAGTC 202 202 . . 202 212 - BplI GAGNNNNNCTC 225 220 193 188 210 215 - BsiI* CACGAG 210 214 . . 229 232 + CviRI* TGCA 230 230 . . 245 249 + BceAI ACGGC 261 263 . . 248 253 + Cac8I GCNNGC 250 250 . . 251 254 + SetI ASST 254 250 . . 251 254 + CviJI RGCY 252 252 . . 251 254 + AluI AGCT 252 252 . . 253 256 + MaeI CTAG 253 255 . . 264 268 + TfiI GAWTC 264 267 . . 264 268 + HinfI GANTC 264 267 . . 267 272 + XbaI TCTAGA 267 271 . . 267 272 + Hpy178III* TCNNGA 268 270 . . 268 271 + MaeI CTAG 268 270 . . 271 275 + HgaI GACGC 280 285 . . 276 281 + TatI WGTACW 276 280 . . 277 280 + Csp6I GTAC 277 279 . . 277 280 + RsaI GTAC 278 278 . . 283 286 + CviRI* TGCA 284 284 . . 286 291 + ApoI RAATTY 286 290 . . 287 290 + TspEI AATT 286 290 . . 301 305 + MboII GAAGA 313 312 . . 307 310 + MseI TTAA 307 309 . . 309 314 + HindIII AAGCTT 309 313 . . 310 313 + SetI ASST 313 309 . . 310 313 + CviJI RGCY 311 311 . . 310 313 + AluI AGCT 311 311 . . 313 316 + MseI TTAA 313 315 . . 317 321 + Tsp4CI* ACNGT 319 318 . . 336 340 - BsmAI GTCTC 330 334 . . 366 369 + MaeI CTAG 366 368 . . #--------------------------------------- # # # AluI: # [AGCT] # 58 59 252 # # ApoI: # [RAATTY] # 83 286 # # BalI: # [TGGCCA] # 4 365 # # BbvI: # [GCAGC] # 155 214 # # BceAI: # [ACGGC] # 105 108 156 # # BisI: # [GCNGC] # 144 225 # # BlsI: # [GCNGC] # 145 224 # # BplI: # [GAGNNNNNCTC] # 0 0 32 144 193 # # BseMII: # [CTCAG] # 115 254 # # BsiI*: # [CACGAG] # 159 210 # # BsmAI: # [GTCTC] # 39 330 # # BspCNI: # [CTCAG] # 114 255 # # BstKTI: # [GATC] # 163 206 # # Cac8I: # [GCNNGC] # 6 119 244 # # CfrI: # [YGGCCR] # 2 367 # # Csp6I: # [GTAC] # 92 277 # # CviJI: # [RGCY] # 4 58 59 248 # # CviRI*: # [TGCA] # 54 85 230 # # DdeI: # [CTNAG] # 101 268 # # DpnI: # [GATC] # 164 205 # # GlaI: # [GCGC] # 74 295 # # HaeIII: # [GGCC] # 4 365 # # HgaI: # [GACGC] # 89 280 # # HhaI: # [GCGC] # 75 294 # # Hin6I: # [GCGC] # 73 296 # # HindII: # [GTYRAC] # 24 345 # # HindIII: # [AAGCTT] # 60 309 # # HinfI: # [GANTC] # 56 97 105 111 # # Hpy166II: # [GTNNAC] # 24 345 # # Hpy178III*: # [TCNNGA] # 101 268 # # MaeI: # [CTAG] # 3 15 98 253 # # MaeII: # [ACGT] # 44 119 206 # # MboI: # [GATC] # 166 203 # # MboII: # [GAAGA] # 56 313 # # MlyI: # [GAGTC] # 167 202 # # MmeI: # [TCCRAC] # 65 304 # # MnlI: # [CCTC] # 141 228 # # MseI: # [TTAA] # 6 56 307 # # PleI: # [GAGTC] # 167 202 # # RsaI: # [GTAC] # 91 278 # # SetI: # [ASST] # 14 47 56 59 88 105 # # SspI: # [AATATT] # 174 195 # # TaiI: # [ACGT] # 47 119 203 # # TaqI: # [TCGA] # 73 133 163 # # TatI: # [WGTACW] # 93 276 # # TfiI: # [GAWTC] # 97 105 167 # # TseI: # [GCWGC] # 143 226 # # Tsp4CI*: # [ACNGT] # 50 319 # # TspDTI: # [ATGAA] # 167 202 # # TspEI: # [AATT] # 83 286 # # TspRI: # [CASTGNN] # 99 270 # # XbaI: # [TCTAGA] # 102 267 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 369 # Reported_sequences: 1 # Reported_hitcount: 72 #---------------------------------------