Restriction Fragments for QCR8/YJL166W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

QCR8/YJL166W on chromosome X from coordinates 106434 to 106718.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.28378.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 3 9 + PpuMI RGGWCCY 4 7 . . 3 9 + DraII RGGNCCY 4 7 . . 3 8 + NlaIV GGNNCC 5 5 . . 4 8 + BmgT120I GGNCC 5 6 . . 4 8 + AvaII GGWCC 4 7 . . 4 8 + AsuI* GGNCC 4 7 . . 7 10 + MnlI CCTC 17 16 . . 7 17 + BsiYI* CCNNNNNNNGG 13 10 . . 14 17 - AciI CCGC 14 16 . . 27 30 + FatI CATG 26 30 . . 27 30 + NlaIII CATG 30 26 . . 27 30 + CviAII CATG 27 29 . . 31 35 + FokI GGATG 44 48 . . 31 35 + BseGI GGATG 37 35 . . 32 36 - BccI CCATC 26 27 . . 41 45 + Tsp45I GTSAC 40 45 . . 41 45 + MaeIII GTNAC 40 45 . . 43 52 + MslI CAYNNNNRTG 47 47 . . 43 52 + OliI CACNNNNGTG 47 47 . . 45 48 + FatI CATG 44 48 . . 45 48 + NlaIII CATG 48 44 . . 45 48 + CviAII CATG 45 47 . . 47 52 - TaqII CACCCA 37 35 . . 52 56 + BmgT120I GGNCC 53 54 . . 52 56 + AvaII GGWCC 52 55 . . 52 56 + AsuI* GGNCC 52 55 . . 66 69 + SetI ASST 69 65 . . 73 76 + SetI ASST 76 72 . . 74 77 + MnlI CCTC 84 83 . . 77 82 + NdeI CATATG 78 80 . . 87 91 + BsmAI GTCTC 92 96 . . 92 97 + NdeI CATATG 93 95 . . 104 107 + CviJI RGCY 105 105 . . 106 116 + BsiYI* CCNNNNNNNGG 112 109 . . 114 117 + SetI ASST 117 113 . . 149 153 + MboII GAAGA 161 160 . . 155 158 + MseI TTAA 155 157 . . 159 163 + BsmAI GTCTC 164 168 . . 164 167 + TspEI AATT 163 167 . . 177 182 + VspI ATTAAT 178 180 . . 178 181 + MseI TTAA 178 180 . . 183 186 + SetI ASST 186 182 . . 183 188 + BspMI ACCTGC 192 196 . . 187 190 - AciI CCGC 187 189 . . 187 191 - FauI CCCGC 180 182 . . 191 196 + ApoI RAATTY 191 195 . . 192 195 + TspEI AATT 191 195 . . 201 204 + Csp6I GTAC 201 203 . . 201 204 + RsaI GTAC 202 202 . . 203 207 + BsrI ACTGG 208 206 . . 210 214 + MboII GAAGA 222 221 . . 215 219 + Tsp4CI* ACNGT 217 216 . . 218 222 + MaeIII GTNAC 217 222 . . 230 234 + TspDTI ATGAA 245 243 . . 232 237 + ApoI RAATTY 232 236 . . 233 236 + TspEI AATT 232 236 . . 239 244 + TatI WGTACW 239 243 . . 239 244 + Bsp1407I TGTACA 239 243 . . 240 243 + Csp6I GTAC 240 242 . . 240 243 + RsaI GTAC 241 241 . . 249 252 + SetI ASST 252 248 . . 249 252 + CviJI RGCY 250 250 . . 249 252 + AluI AGCT 250 250 . . 259 263 + MboII GAAGA 271 270 . . 263 266 + SetI ASST 266 262 . . 263 266 + CviJI RGCY 264 264 . . 263 266 + AluI AGCT 264 264 . . 259 264 - Ksp632I* CTCTTC 254 257 . . 259 265 - SapI GCTCTTC 254 257 . . 275 278 + MseI TTAA 275 277 . . 282 285 + MseI TTAA 282 284 . . #--------------------------------------- # # # AciI: # [CCGC] # 14 98 173 # # AluI: # [AGCT] # 14 21 250 # # ApoI: # [RAATTY] # 41 53 191 # # AsuI*: # [GGNCC] # 4 48 233 # # AvaII: # [GGWCC] # 4 48 233 # # BccI: # [CCATC] # 26 259 # # BmgT120I: # [GGNCC] # 5 48 232 # # BseGI: # [GGATG] # 37 248 # # BsiYI*: # [CCNNNNNNNGG] # 13 99 173 # # BsmAI: # [GTCTC] # 72 92 121 # # Bsp1407I: # [TGTACA] # 46 239 # # BspMI: # [ACCTGC] # 93 192 # # BsrI: # [ACTGG] # 77 208 # # Csp6I: # [GTAC] # 39 45 201 # # CviAII: # [CATG] # 18 27 240 # # CviJI: # [RGCY] # 14 21 105 145 # # DraII: # [RGGNCCY] # 4 281 # # FatI: # [CATG] # 18 26 241 # # FauI: # [CCCGC] # 105 180 # # FokI: # [GGATG] # 44 241 # # Ksp632I*: # [CTCTTC] # 31 254 # # MaeIII: # [GTNAC] # 40 68 177 # # MboII: # [GAAGA] # 14 49 61 161 # # MnlI: # [CCTC] # 17 67 201 # # MseI: # [TTAA] # 3 7 23 97 155 # # MslI: # [CAYNNNNRTG] # 47 238 # # NdeI: # [CATATG] # 15 78 192 # # NlaIII: # [CATG] # 18 30 237 # # NlaIV: # [GGNNCC] # 5 280 # # OliI: # [CACNNNNGTG] # 47 238 # # PpuMI: # [RGGWCCY] # 4 281 # # RsaI: # [GTAC] # 39 44 202 # # SapI: # [GCTCTTC] # 31 254 # # SetI: # [ASST] # 7 14 19 41 66 69 # 69 # # TaqII: # [CACCCA] # 37 248 # # TatI: # [WGTACW] # 46 239 # # Tsp45I: # [GTSAC] # 40 245 # # Tsp4CI*: # [ACNGT] # 68 217 # # TspDTI: # [ATGAA] # 40 245 # # TspEI: # [AATT] # 28 41 53 163 # # VspI: # [ATTAAT] # 107 178 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 285 # Reported_sequences: 1 # Reported_hitcount: 71 #---------------------------------------