Restriction Fragments for CIS3/YJL158C



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The currently selected gene/sequence is :

CIS3/YJL158C on chromosome X from coordinates 122948 to 122265.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.10782.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 2 5 + CviRI* TGCA 3 3 . . 5 8 + TspEI AATT 4 8 . . 14 17 + TaiI ACGT 17 13 . . 14 17 + SetI ASST 17 13 . . 14 17 + MaeII ACGT 14 16 . . 15 19 + Hpy99I CGWCG 19 14 . . 19 29 + MwoI GCNNNNNNNGC 25 22 . . 22 25 + MaeI CTAG 22 24 . . 24 27 + SetI ASST 27 23 . . 24 27 + CviJI RGCY 25 25 . . 24 27 + AluI AGCT 25 25 . . 25 29 + BlsI GCNGC 27 26 . . 25 29 + BisI GCNGC 26 27 . . 25 29 + TseI GCWGC 25 28 . . 28 38 + MwoI GCNNNNNNNGC 34 31 . . 29 32 + MnlI CCTC 39 38 . . 25 29 - BbvI GCAGC 12 16 . . 31 35 - TspGWI ACGGA 21 19 . . 37 41 + BlsI GCNGC 39 38 . . 37 41 + BisI GCNGC 38 39 . . 37 41 + TseI GCWGC 37 40 . . 40 50 + MwoI GCNNNNNNNGC 46 43 . . 37 41 - BbvI GCAGC 24 28 . . 47 50 + AciI CCGC 47 49 . . 51 57 + TspRI CASTGNN 57 48 . . 46 51 - EciI GGCGGA 36 34 . . 51 56 - BtsI GCAGTG 50 48 . . 62 67 + Eco57MI CTGRAG 83 81 . . 62 67 + Eco57I CTGAAG 83 81 . . 59 64 - EcoP15I CAGCAG 31 33 . . 66 69 + SetI ASST 69 65 . . 68 72 + MaeIII GTNAC 67 72 . . 64 68 - Hin4II* CCTTC 58 57 . . 76 90 + XcmI CCANNNNNNNNNTGG 83 82 . . 76 80 + BssKI CCNGG 75 80 . . 76 80 + BseBI CCWGG 77 78 . . 76 80 + ScrFI CCNGG 77 78 . . 76 80 + EcoRII CCWGG 75 80 . . 78 81 + SetI ASST 81 77 . . 74 79 - Eco57MI CTGRAG 59 57 . . 74 79 - GsuI CTGGAG 59 57 . . 79 83 + HphI GGTGA 91 90 . . 80 85 + Hpy166II GTNNAC 82 82 . . 85 90 + DsaI* CCRYGG 85 89 . . 85 90 + StyI CCWWGG 85 89 . . 85 90 + NcoI CCATGG 85 89 . . 85 90 + SecI* CCNNGG 85 89 . . 86 89 + FatI CATG 85 89 . . 86 89 + NlaIII CATG 89 85 . . 86 89 + CviAII CATG 86 88 . . 89 93 + BmgT120I GGNCC 90 91 . . 89 93 + AvaII GGWCC 89 92 . . 89 93 + AsuI* GGNCC 89 92 . . 90 95 + Hpy166II GTNNAC 92 92 . . 94 97 + SetI ASST 97 93 . . 97 100 + MseI TTAA 97 99 . . 106 111 + Cfr10I RCCGGY 106 110 . . 107 110 + HpaII CCGG 107 109 . . 109 112 + CviJI RGCY 110 110 . . 109 114 + NlaIV GGNNCC 111 111 . . 113 117 + BccI CCATC 121 122 . . 127 131 + BlsI GCNGC 129 128 . . 127 131 + BisI GCNGC 128 129 . . 127 131 + TseI GCWGC 127 130 . . 127 131 - BbvI GCAGC 114 118 . . 145 148 + SetI ASST 148 144 . . 157 167 + MwoI GCNNNNNNNGC 163 160 . . 165 168 + SetI ASST 168 164 . . 165 168 + CviJI RGCY 166 166 . . 165 168 + AluI AGCT 166 166 . . 172 175 + SetI ASST 175 171 . . 173 176 + MnlI CCTC 183 182 . . 174 179 + Ksp632I* CTCTTC 180 183 . . 175 179 - MboII GAAGA 167 166 . . 183 186 + SetI ASST 186 182 . . 183 186 + CviJI RGCY 184 184 . . 183 186 + AluI AGCT 184 184 . . 185 189 + DdeI CTNAG 185 188 . . 193 203 + Hin4I GAYNNNNNVTC 184 179 216 211 194 197 + TaiI ACGT 197 193 . . 194 197 + SetI ASST 197 193 . . 194 197 + MaeII ACGT 194 196 . . 191 195 - BsmAI GTCTC 185 189 . . 191 196 - Esp3I CGTCTC 185 189 . . 191 201 - Hin4I GAYNNNNNVTC 214 209 182 177 207 210 + TspEI AATT 206 210 . . 211 215 + HphI GGTGA 223 222 . . 212 216 + Tsp45I GTSAC 211 216 . . 212 216 + MaeIII GTNAC 211 216 . . 214 225 + DrdI GACNNNNNNGTC 220 218 . . 215 219 + Tsp4CI* ACNGT 217 216 . . 228 231 + CviJI RGCY 229 229 . . 229 239 + MwoI GCNNNNNNNGC 235 232 . . 238 248 + MwoI GCNNNNNNNGC 244 241 . . 238 242 + BlsI GCNGC 240 239 . . 238 242 + BisI GCNGC 239 240 . . 238 242 + TseI GCWGC 238 241 . . 236 241 - EcoP15I CAGCAG 208 210 . . 238 242 - BbvI GCAGC 225 229 . . 248 253 + SmlI CTYRAG 248 252 . . 252 255 + CviJI RGCY 253 253 . . 248 253 - Bce83I* CTTGAG 233 231 . . 267 270 + CviJI RGCY 268 268 . . 273 276 + SetI ASST 276 272 . . 273 276 + CviJI RGCY 274 274 . . 273 276 + AluI AGCT 274 274 . . 284 287 + AciI CCGC 284 286 . . 298 301 + SetI ASST 301 297 . . 298 301 + CviJI RGCY 299 299 . . 298 301 + AluI AGCT 299 299 . . 301 305 + Hpy188I TCNGA 303 302 . . 308 313 + XhoII RGATCY 308 312 . . 308 313 + BglII AGATCT 308 312 . . 309 312 + DpnI GATC 310 310 . . 309 312 + BstKTI GATC 311 309 . . 309 312 + MboI GATC 308 312 . . 312 317 + Ksp632I* CTCTTC 318 321 . . 317 320 + MnlI CCTC 327 326 . . 313 317 - MboII GAAGA 305 304 . . 321 324 + CviRI* TGCA 322 322 . . 322 326 + SfaNI GCATC 331 335 . . 352 356 - MboII GAAGA 344 343 . . 367 371 + BccI CCATC 375 376 . . 382 385 + SetI ASST 385 381 . . 382 385 + CviJI RGCY 383 383 . . 382 385 + AluI AGCT 383 383 . . 386 389 + FatI CATG 385 389 . . 386 389 + NlaIII CATG 389 385 . . 386 389 + CviAII CATG 386 388 . . 393 398 + EcoRI GAATTC 393 397 . . 393 398 + ApoI RAATTY 393 397 . . 394 397 + TspEI AATT 393 397 . . 400 405 + KpnI GGTACC 404 400 . . 400 405 + NlaIV GGNNCC 402 402 . . 400 405 + HgiCI* GGYRCC 400 404 . . 400 405 + Acc65I GGTACC 400 404 . . 401 404 + Csp6I GTAC 401 403 . . 401 404 + RsaI GTAC 402 402 . . 403 406 + SetI ASST 406 402 . . 405 409 + DdeI CTNAG 405 408 . . 410 413 + TspEI AATT 409 413 . . 415 418 + SetI ASST 418 414 . . 420 424 - Hin4II* CCTTC 414 413 . . 425 429 + Tsp4CI* ACNGT 427 426 . . 439 443 - SfaNI GCATC 429 433 . . 443 448 + StyI CCWWGG 443 447 . . 443 448 + SecI* CCNNGG 443 447 . . 452 461 + XmnI GAANNNNTTC 456 456 . . 453 456 + TspEI AATT 452 456 . . 449 461 - PsrI GAACNNNNNNTAC 473 468 441 436 479 482 + TspEI AATT 478 482 . . 485 488 + TspEI AATT 484 488 . . 491 495 + Tsp4CI* ACNGT 493 492 . . 493 497 + BmgT120I GGNCC 494 495 . . 493 497 + AvaII GGWCC 493 496 . . 493 497 + AsuI* GGNCC 493 496 . . 494 499 + Hpy166II GTNNAC 496 496 . . 502 512 + BsiYI* CCNNNNNNNGG 508 505 . . 507 510 + CviJI RGCY 508 508 . . 508 513 + NaeI GCCGGC 510 510 . . 508 513 + MroNI GCCGGC 508 512 . . 508 513 + Cac8I GCNNGC 510 510 . . 508 513 + Cfr10I RCCGGY 508 512 . . 509 512 + HpaII CCGG 509 511 . . 511 516 + NarI GGCGCC 512 514 . . 511 516 + KasI GGCGCC 511 515 . . 511 516 + DinI GGCGCC 513 513 . . 511 516 + HaeII RGCGCY 515 511 . . 511 516 + NlaIV GGNNCC 513 513 . . 511 516 + AcyI GRCGYC 512 514 . . 511 516 + HgiCI* GGYRCC 511 515 . . 512 515 + Hin6I GCGC 512 514 . . 512 515 + GlaI GCGC 513 513 . . 512 515 + HhaI GCGC 514 512 . . 514 524 + MwoI GCNNNNNNNGC 520 517 . . 515 519 + BccI CCATC 523 524 . . 523 527 + BlsI GCNGC 525 524 . . 523 527 + BisI GCNGC 524 525 . . 523 527 + TseI GCWGC 523 526 . . 526 531 + Cfr10I RCCGGY 526 530 . . 527 530 + HpaII CCGG 527 529 . . 523 527 - BbvI GCAGC 510 514 . . 533 537 + BmgT120I GGNCC 534 535 . . 533 537 + AvaII GGWCC 533 536 . . 533 537 + AsuI* GGNCC 533 536 . . 542 547 + Eco57MI CTGRAG 563 561 . . 542 547 + Eco57I CTGAAG 563 561 . . 544 548 + MboII GAAGA 556 555 . . 551 555 + MaeIII GTNAC 550 555 . . 547 551 - BccI CCATC 541 542 . . 549 554 - TsoI TARCCA 539 537 . . 558 561 + CviJI RGCY 559 559 . . 565 569 + HphI GGTGA 577 576 . . 566 570 + Tsp45I GTSAC 565 570 . . 566 570 + MaeIII GTNAC 565 570 . . 563 568 - TaqII CACCCA 553 551 . . 569 573 + Tsp4CI* ACNGT 571 570 . . 570 576 + TspRI CASTGNN 576 567 . . 572 576 + Tsp45I GTSAC 571 576 . . 572 576 + MaeIII GTNAC 571 576 . . 575 578 + TaiI ACGT 578 574 . . 575 578 + SetI ASST 578 574 . . 575 578 + MaeII ACGT 575 577 . . 596 599 + HpaII CCGG 596 598 . . 601 604 + TspEI AATT 600 604 . . 618 623 + BclI TGATCA 618 622 . . 619 622 + DpnI GATC 620 620 . . 619 622 + BstKTI GATC 621 619 . . 619 622 + MboI GATC 618 622 . . 626 629 + TaiI ACGT 629 625 . . 626 629 + SetI ASST 629 625 . . 626 629 + MaeII ACGT 626 628 . . 627 631 + Hpy99I CGWCG 631 626 . . 647 658 + BstXI CCANNNNNNTGG 654 650 . . 650 654 - TspDTI ATGAA 640 638 . . 660 663 + SetI ASST 663 659 . . 660 663 + CviJI RGCY 661 661 . . 660 663 + AluI AGCT 661 661 . . 673 678 + SalI GTCGAC 673 677 . . 673 678 + Hpy166II GTNNAC 675 675 . . 673 678 + HindII GTYRAC 675 675 . . 673 678 + AccI GTMKAC 674 676 . . 674 677 + TaqI TCGA 674 676 . . 677 681 + Tsp4CI* ACNGT 679 678 . . 681 684 + MseI TTAA 681 683 . . #--------------------------------------- # # # Acc65I: # [GGTACC] # 284 400 # # AccI: # [GTMKAC] # 10 674 # # AciI: # [CCGC] # 47 237 400 # # AcyI: # [GRCGYC] # 172 512 # # AluI: # [AGCT] # 18 23 25 25 84 90 # 141 278 # # ApoI: # [RAATTY] # 291 393 # # AsuI*: # [GGNCC] # 40 89 151 404 # # AvaII: # [GGWCC] # 40 89 151 404 # # BbvI: # [GCAGC] # 12 12 90 111 174 285 # # BccI: # [CCATC] # 18 121 143 148 254 # # Bce83I*: # [CTTGAG] # 233 451 # # BclI: # [TGATCA] # 66 618 # # BglII: # [AGATCT] # 308 376 # # BisI: # [GCNGC] # 12 26 90 111 160 285 # # BlsI: # [GCNGC] # 12 27 90 111 159 285 # # BmgT120I: # [GGNCC] # 40 90 150 404 # # BseBI: # [CCWGG] # 77 607 # # BsiYI*: # [CCNNNNNNNGG] # 176 508 # # BsmAI: # [GTCTC] # 185 499 # # BssKI: # [CCNGG] # 75 609 # # BstKTI: # [GATC] # 63 310 311 # # BstXI: # [CCANNNNNNTGG] # 30 654 # # BtsI: # [GCAGTG] # 50 634 # # Cac8I: # [GCNNGC] # 174 510 # # Cfr10I: # [RCCGGY] # 18 106 158 402 # # Csp6I: # [GTAC] # 283 401 # # CviAII: # [CATG] # 86 298 300 # # CviJI: # [RGCY] # 6 15 18 23 24 25 # 25 45 51 56 84 85 # 102 125 # # CviRI*: # [TGCA] # 3 319 362 # # DdeI: # [CTNAG] # 185 220 279 # # DinI: # [GGCGCC] # 171 513 # # DpnI: # [GATC] # 64 310 310 # # DrdI: # [GACNNNNNNGTC] # 220 464 # # DsaI*: # [CCRYGG] # 85 599 # # EciI: # [GGCGGA] # 36 648 # # Eco57I: # [CTGAAG] # 83 121 480 # # Eco57MI: # [CTGRAG] # 24 59 121 480 # # EcoP15I: # [CAGCAG] # 31 177 476 # # EcoRI: # [GAATTC] # 291 393 # # EcoRII: # [CCWGG] # 75 609 # # Esp3I: # [CGTCTC] # 185 499 # # FatI: # [CATG] # 85 299 300 # # GlaI: # [GCGC] # 171 513 # # GsuI: # [CTGGAG] # 59 625 # # HaeII: # [RGCGCY] # 169 515 # # HgiCI*: # [GGYRCC] # 111 173 400 # # HhaI: # [GCGC] # 170 514 # # Hin4I: # [GAYNNNNNVTC] # 2 2 30 182 468 # # Hin4II*: # [CCTTC] # 58 270 356 # # Hin6I: # [GCGC] # 172 512 # # HindII: # [GTYRAC] # 9 675 # # HpaII: # [CCGG] # 18 69 88 107 402 # # HphI: # [GGTGA] # 91 107 132 354 # # Hpy166II: # [GTNNAC] # 9 10 82 179 404 # # Hpy188I: # [TCNGA] # 303 381 # # Hpy99I: # [CGWCG] # 19 53 612 # # KasI: # [GGCGCC] # 173 511 # # KpnI: # [GGTACC] # 280 404 # # Ksp632I*: # [CTCTTC] # 138 180 366 # # MaeI: # [CTAG] # 22 662 # # MaeII: # [ACGT] # 14 51 58 180 381 # # MaeIII: # [GTNAC] # 6 15 67 113 144 339 # # MboI: # [GATC] # 66 308 310 # # MboII: # [GAAGA] # 39 128 138 167 212 # # MnlI: # [CCTC] # 39 144 144 357 # # MroNI: # [GCCGGC] # 176 508 # # MseI: # [TTAA] # 3 97 584 # # MwoI: # [GCNNNNNNNGC] # 9 9 12 25 72 117 # 164 276 # # NaeI: # [GCCGGC] # 174 510 # # NarI: # [GGCGCC] # 172 512 # # NcoI: # [CCATGG] # 85 599 # # NlaIII: # [CATG] # 89 295 300 # # NlaIV: # [GGNNCC] # 111 111 171 291 # # PsrI: # [GAACNNNNNNTAC] # 32 211 441 # # RsaI: # [GTAC] # 282 402 # # SalI: # [GTCGAC] # 11 673 # # ScrFI: # [CCNGG] # 77 607 # # SecI*: # [CCNNGG] # 85 241 358 # # SetI: # [ASST] # 7 10 11 11 12 12 # 16 17 20 21 21 25 # 34 42 51 51 79 84 # 160 # # SfaNI: # [GCATC] # 98 255 331 # # SmlI: # [CTYRAG] # 248 436 # # StyI: # [CCWWGG] # 85 241 358 # # TaiI: # [ACGT] # 17 51 55 180 381 # # TaqI: # [TCGA] # 10 674 # # TaqII: # [CACCCA] # 131 553 # # TseI: # [GCWGC] # 12 25 90 111 161 285 # # TsoI: # [TARCCA] # 145 539 # # Tsp45I: # [GTSAC] # 6 113 211 354 # # Tsp4CI*: # [ACNGT] # 5 66 78 108 210 217 # # TspDTI: # [ATGAA] # 44 640 # # TspEI: # [AATT] # 4 6 16 26 43 84 # 116 187 202 # # TspGWI: # [ACGGA] # 21 663 # # TspRI: # [CASTGNN] # 57 108 519 # # XcmI: # [CCANNNNNNNNNTGG] # 83 601 # # XhoII: # [RGATCY] # 308 376 # # XmnI: # [GAANNNNTTC] # 228 456 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 684 # Reported_sequences: 1 # Reported_hitcount: 225 #---------------------------------------