Restriction Fragments for VPS35/YJL154C



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The currently selected gene/sequence is :

VPS35/YJL154C on chromosome X from coordinates 133935 to 131101.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.25536.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 13 17 + HinfI GANTC 13 16 . . 10 13 - AciI CCGC 10 12 . . 13 17 - PleI GAGTC 7 8 . . 13 17 - MlyI GAGTC 7 7 . . 16 20 - HphI GGTGA 8 7 . . 28 35 + SgfI GCGATCGC 32 30 . . 29 34 + McrI* CGRYCG 32 30 . . 29 34 + PvuI CGATCG 32 30 . . 30 33 + DpnI GATC 31 31 . . 30 33 + BstKTI GATC 32 30 . . 30 33 + MboI GATC 29 33 . . 45 49 + BlsI GCNGC 47 46 . . 45 49 + BisI GCNGC 46 47 . . 45 49 + BbvI GCAGC 57 61 . . 45 49 + TseI GCWGC 45 48 . . 53 56 + AciI CCGC 53 55 . . 61 65 + TspDTI ATGAA 76 74 . . 65 70 + Cfr10I RCCGGY 65 69 . . 65 70 + BetI* WCCGGW 65 69 . . 65 70 + AgeI ACCGGT 65 69 . . 66 69 + HpaII CCGG 66 68 . . 94 98 + TfiI GAWTC 94 97 . . 94 98 + HinfI GANTC 94 97 . . 120 125 + Hpy166II GTNNAC 122 122 . . 120 125 + HindII GTYRAC 122 122 . . 123 128 + TaqII GACCGA 139 137 . . 128 131 + TspEI AATT 127 131 . . 153 157 + MboII GAAGA 165 164 . . 153 165 + BarI GAAGNNNNNNTAC 145 140 177 172 185 188 + TaqI TCGA 185 187 . . 187 191 + HinfI GANTC 187 190 . . 187 191 - PleI GAGTC 181 182 . . 187 191 - MlyI GAGTC 181 181 . . 207 212 + SnaBI TACGTA 209 209 . . 207 212 + BsaAI YACGTR 209 209 . . 208 211 + TaiI ACGT 211 207 . . 208 211 + SetI ASST 211 207 . . 208 211 + MaeII ACGT 208 210 . . 210 213 + Csp6I GTAC 210 212 . . 210 213 + RsaI GTAC 211 211 . . 212 215 + SetI ASST 215 211 . . 213 216 + MnlI CCTC 223 222 . . 225 229 + BccI CCATC 233 234 . . 229 232 + MnlI CCTC 239 238 . . 226 230 - FokI GGATG 212 216 . . 226 230 - BseGI GGATG 225 223 . . 237 241 - HphI GGTGA 229 228 . . 243 247 + DdeI CTNAG 243 246 . . 246 249 + SetI ASST 249 245 . . 246 249 + CviJI RGCY 247 247 . . 246 249 + AluI AGCT 247 247 . . 250 253 + DpnI GATC 251 251 . . 250 253 + BstKTI GATC 252 250 . . 250 253 + MboI GATC 249 253 . . 258 269 + BdaI TGANNNNNNTCA 247 245 281 279 258 269 - BdaI TGANNNNNNTCA 281 279 247 245 274 279 + Cfr10I RCCGGY 274 278 . . 274 279 + BetI* WCCGGW 274 278 . . 274 279 + AgeI ACCGGT 274 278 . . 275 278 + HpaII CCGG 275 277 . . 278 282 + MaeIII GTNAC 277 282 . . 281 284 + TaiI ACGT 284 280 . . 281 284 + SetI ASST 284 280 . . 281 284 + MaeII ACGT 281 283 . . 285 290 + KpnI GGTACC 289 285 . . 285 290 + NlaIV GGNNCC 287 287 . . 285 290 + HgiCI* GGYRCC 285 289 . . 285 290 + Acc65I GGTACC 285 289 . . 286 289 + Csp6I GTAC 286 288 . . 286 289 + RsaI GTAC 287 287 . . 289 294 + SecI* CCNNGG 289 293 . . 290 294 + BssKI CCNGG 289 294 . . 290 294 + BseBI CCWGG 291 292 . . 290 294 + ScrFI CCNGG 291 292 . . 290 294 + EcoRII CCWGG 289 294 . . 293 296 + CviJI RGCY 294 294 . . 305 310 + BclI TGATCA 305 309 . . 306 309 + DpnI GATC 307 307 . . 306 309 + BstKTI GATC 308 306 . . 306 309 + MboI GATC 305 309 . . 310 314 + Tsp4CI* ACNGT 312 311 . . 316 320 + BslFI GGGAC 330 334 . . 316 321 + NlaIV GGNNCC 318 318 . . 317 321 + BmgT120I GGNCC 318 319 . . 317 321 + AvaII GGWCC 317 320 . . 317 321 + AsuI* GGNCC 317 320 . . 318 328 + Hin4I GAYNNNNNVTC 309 304 341 336 322 325 + SetI ASST 325 321 . . 322 325 + CviJI RGCY 323 323 . . 322 325 + AluI AGCT 323 323 . . 318 328 - Hin4I GAYNNNNNVTC 341 336 309 304 338 342 + TspDTI ATGAA 353 351 . . 342 347 + HaeII RGCGCY 346 342 . . 343 346 + Hin6I GCGC 343 345 . . 343 346 + GlaI GCGC 344 344 . . 343 346 + HhaI GCGC 345 343 . . 352 362 + FalI AAGNNNNNCTT 343 338 375 370 351 355 - Hin4II* CCTTC 345 344 . . 361 364 + MseI TTAA 361 363 . . 352 362 - FalI AAGNNNNNCTT 375 370 343 338 392 395 + CviRI* TGCA 393 393 . . 400 410 + BsiYI* CCNNNNNNNGG 406 403 . . 408 411 + SetI ASST 411 407 . . 407 410 - MnlI CCTC 400 399 . . 446 456 + FalI AAGNNNNNCTT 437 432 469 464 448 457 + XmnI GAANNNNTTC 452 452 . . 449 452 + TspEI AATT 448 452 . . 456 461 + Hpy178III* TCNNGA 457 459 . . 446 456 - FalI AAGNNNNNCTT 469 464 437 432 456 461 + BetI* WCCGGW 456 460 . . 456 461 + BspMII* TCCGGA 456 460 . . 457 460 + HpaII CCGG 457 459 . . 460 463 - MnlI CCTC 453 452 . . 463 473 + Hin4I GAYNNNNNVTC 454 449 486 481 463 473 + Eam1105I GACNNNNNGTC 468 467 . . 466 469 + DpnI GATC 467 467 . . 466 469 + BstKTI GATC 468 466 . . 466 469 + MboI GATC 465 469 . . 470 474 + Hpy99I CGWCG 474 469 . . 466 470 - BinI* GGATC 460 461 . . 468 472 - TspGWI ACGGA 458 456 . . 463 473 - Hin4I GAYNNNNNVTC 486 481 454 449 476 479 + MseI TTAA 476 478 . . 485 488 + TspEI AATT 484 488 . . 493 497 + TspDTI ATGAA 508 506 . . 494 505 + BdaI TGANNNNNNTCA 483 481 517 515 495 504 + XmnI GAANNNNTTC 499 499 . . 499 502 + TspEI AATT 498 502 . . 504 513 + MslI CAYNNNNRTG 508 508 . . 494 505 - BdaI TGANNNNNNTCA 517 515 483 481 502 506 - TspDTI ATGAA 492 490 . . 506 509 + TaqI TCGA 506 508 . . 506 511 + Hpy178III* TCNNGA 507 509 . . 511 515 + TspDTI ATGAA 526 524 . . 525 529 + HphI GGTGA 537 536 . . 523 528 - TaqII CACCCA 513 511 . . 540 544 + BssKI CCNGG 539 544 . . 540 544 + BseBI CCWGG 541 542 . . 540 545 + SecI* CCNNGG 540 544 . . 540 544 + ScrFI CCNGG 541 542 . . 540 544 + EcoRII CCWGG 539 544 . . 543 548 + NlaIV GGNNCC 545 545 . . 544 548 + BmgT120I GGNCC 545 546 . . 544 548 + AsuI* GGNCC 544 547 . . 545 548 + CviJI RGCY 546 546 . . 545 548 + HaeIII GGCC 546 546 . . 548 556 + DraIII CACNNNGTG 553 550 . . 552 557 + Hpy178III* TCNNGA 553 555 . . 558 561 - MnlI CCTC 551 550 . . 571 576 + AflIII ACRYGT 571 575 . . 572 577 + BsaAI YACGTR 574 574 . . 572 577 + PmaCI CACGTG 574 574 . . 573 576 + TaiI ACGT 576 572 . . 573 576 + SetI ASST 576 572 . . 573 576 + MaeII ACGT 573 575 . . 587 590 + SetI ASST 590 586 . . 587 590 + CviJI RGCY 588 588 . . 587 590 + AluI AGCT 588 588 . . 588 592 + BlsI GCNGC 590 589 . . 588 592 + BisI GCNGC 589 590 . . 588 592 + TseI GCWGC 588 591 . . 590 593 + CviRI* TGCA 591 591 . . 588 592 - BbvI GCAGC 575 579 . . 593 598 + ApoI RAATTY 593 597 . . 594 597 + TspEI AATT 593 597 . . 605 610 + Eco31I GGTCTC 611 615 . . 606 610 + BsmAI GTCTC 611 615 . . 612 617 + SpeI ACTAGT 612 616 . . 613 616 + MaeI CTAG 613 615 . . 616 619 + Csp6I GTAC 616 618 . . 616 619 + RsaI GTAC 617 617 . . 618 621 + TaiI ACGT 621 617 . . 618 621 + SetI ASST 621 617 . . 618 621 + MaeII ACGT 618 620 . . 643 646 + TspEI AATT 642 646 . . 662 672 + FalI AAGNNNNNCTT 653 648 685 680 662 672 - FalI AAGNNNNNCTT 685 680 653 648 669 673 - MboII GAAGA 661 660 . . 674 685 + BstXI CCANNNNNNTGG 681 677 . . 685 697 + PsrI GAACNNNNNNTAC 677 672 709 704 706 716 + Hin4I GAYNNNNNVTC 697 692 729 724 712 717 + BciVI GTATCC 723 722 . . 706 716 - Hin4I GAYNNNNNVTC 729 724 697 692 719 729 + FalI AAGNNNNNCTT 710 705 742 737 719 729 - FalI AAGNNNNNCTT 742 737 710 705 733 738 + ZraI GACGTC 735 735 . . 733 738 + AcyI GRCGYC 734 736 . . 733 738 + AatII GACGTC 737 733 . . 734 737 + TaiI ACGT 737 733 . . 734 737 + SetI ASST 737 733 . . 734 737 + MaeII ACGT 734 736 . . 775 778 + SetI ASST 778 774 . . 776 781 + StyI CCWWGG 776 780 . . 776 781 + SecI* CCNNGG 776 780 . . 780 785 - BciVI GTATCC 774 773 . . 791 794 + CviRI* TGCA 792 792 . . 803 806 + CviRI* TGCA 804 804 . . 823 826 + TaqI TCGA 823 825 . . 851 854 + TaqI TCGA 851 853 . . 852 857 + McrI* CGRYCG 855 853 . . 852 857 + PvuI CGATCG 855 853 . . 853 856 + DpnI GATC 854 854 . . 853 856 + BstKTI GATC 855 853 . . 853 856 + MboI GATC 852 856 . . 854 859 + ClaI ATCGAT 855 857 . . 855 858 + TaqI TCGA 855 857 . . 859 862 + MseI TTAA 859 861 . . 871 875 + MaeIII GTNAC 870 875 . . 879 883 + Tsp4CI* ACNGT 881 880 . . 882 887 - MmeI TCCRAC 863 861 . . 886 889 - MnlI CCTC 879 878 . . 892 895 + DpnI GATC 893 893 . . 892 895 + BstKTI GATC 894 892 . . 892 895 + MboI GATC 891 895 . . 892 896 - BinI* GGATC 886 887 . . 899 909 + BsaXI ACNNNNNCTCC 889 886 919 916 904 907 + SetI ASST 907 903 . . 905 908 + MnlI CCTC 915 914 . . 912 917 + BsmI GAATGC 918 916 . . 932 935 + TaiI ACGT 935 931 . . 932 935 + SetI ASST 935 931 . . 932 935 + MaeII ACGT 932 934 . . 932 937 + AflIII ACRYGT 932 936 . . 931 936 - BtrI CACGTC 933 933 . . 941 944 + Csp6I GTAC 941 943 . . 941 944 + RsaI GTAC 942 942 . . 943 946 + TaiI ACGT 946 942 . . 943 946 + SetI ASST 946 942 . . 943 946 + MaeII ACGT 943 945 . . 950 954 + BslFI GGGAC 964 968 . . 961 965 + Tsp4CI* ACNGT 963 962 . . 969 973 + TfiI GAWTC 969 972 . . 969 973 + HinfI GANTC 969 972 . . 972 977 + Hpy178III* TCNNGA 973 975 . . 972 977 + BspHI TCATGA 972 976 . . 973 976 + FatI CATG 972 976 . . 973 976 + NlaIII CATG 976 972 . . 973 976 + CviAII CATG 973 975 . . 974 978 + TspDTI ATGAA 989 987 . . 984 989 + XhoII RGATCY 984 988 . . 984 989 + BglII AGATCT 984 988 . . 985 988 + DpnI GATC 986 986 . . 985 988 + BstKTI GATC 987 985 . . 985 988 + MboI GATC 984 988 . . 999 1003 + Tsp4CI* ACNGT 1001 1000 . . 1009 1012 + MnlI CCTC 1019 1018 . . 1012 1015 + MaeI CTAG 1012 1014 . . 1033 1036 + MseI TTAA 1033 1035 . . 1047 1052 + BciVI GTATCC 1058 1057 . . 1057 1060 + TspEI AATT 1056 1060 . . 1066 1069 + TspEI AATT 1065 1069 . . 1085 1088 + TspEI AATT 1084 1088 . . 1103 1107 + DdeI CTNAG 1103 1106 . . 1116 1120 + Hpy188I TCNGA 1118 1117 . . 1123 1128 + SspI AATATT 1125 1125 . . 1135 1139 + PleI GAGTC 1143 1144 . . 1135 1139 + MlyI GAGTC 1144 1144 . . 1135 1139 + HinfI GANTC 1135 1138 . . 1138 1142 + Hpy188I TCNGA 1140 1139 . . 1169 1174 + Hpy178III* TCNNGA 1170 1172 . . 1178 1183 + Hpy166II GTNNAC 1180 1180 . . 1187 1190 + SetI ASST 1190 1186 . . 1187 1190 + CviJI RGCY 1188 1188 . . 1187 1190 + AluI AGCT 1188 1188 . . 1183 1188 - Eco57MI CTGRAG 1168 1166 . . 1183 1188 - Eco57I CTGAAG 1168 1166 . . 1192 1195 + SetI ASST 1195 1191 . . 1193 1196 + MnlI CCTC 1203 1202 . . 1198 1201 + TaqI TCGA 1198 1200 . . 1203 1209 + TspRI CASTGNN 1209 1200 . . 1207 1210 + Hin6I GCGC 1207 1209 . . 1207 1210 + GlaI GCGC 1208 1208 . . 1207 1210 + HhaI GCGC 1209 1207 . . 1203 1208 - BtsI GCAGTG 1202 1200 . . 1219 1223 - HphI GGTGA 1211 1210 . . 1225 1229 + MaeIII GTNAC 1224 1229 . . 1222 1226 - BsrI ACTGG 1222 1220 . . 1230 1235 + AsuII TTCGAA 1231 1233 . . 1231 1234 + TaqI TCGA 1231 1233 . . 1253 1256 + TspEI AATT 1252 1256 . . 1254 1259 + VspI ATTAAT 1255 1257 . . 1255 1258 + MseI TTAA 1255 1257 . . 1263 1273 + BsiYI* CCNNNNNNNGG 1269 1266 . . 1264 1270 + TspRI CASTGNN 1270 1261 . . 1263 1267 - BsrI ACTGG 1263 1261 . . 1262 1267 - BfiI ACTGGG 1257 1256 . . 1269 1273 + BssKI CCNGG 1268 1273 . . 1269 1273 + BseBI CCWGG 1270 1271 . . 1269 1273 + ScrFI CCNGG 1270 1271 . . 1269 1273 + EcoRII CCWGG 1268 1273 . . 1272 1275 + CviJI RGCY 1273 1273 . . 1287 1291 + BssKI CCNGG 1286 1291 . . 1287 1291 + BseBI CCWGG 1288 1289 . . 1287 1291 + ScrFI CCNGG 1288 1289 . . 1287 1291 + EcoRII CCWGG 1286 1291 . . 1284 1288 - FokI GGATG 1270 1274 . . 1284 1288 - BseGI GGATG 1283 1281 . . 1290 1293 + CviJI RGCY 1291 1291 . . 1295 1298 + CviRI* TGCA 1296 1296 . . 1307 1312 + Hpy166II GTNNAC 1309 1309 . . 1307 1312 + AccI GTMKAC 1308 1310 . . 1299 1309 - Hin4I GAYNNNNNVTC 1322 1317 1290 1285 1328 1332 + TspDTI ATGAA 1343 1341 . . 1332 1335 + TspEI AATT 1331 1335 . . 1341 1350 + MslI CAYNNNNRTG 1345 1345 . . 1345 1348 + MseI TTAA 1345 1347 . . 1363 1367 + MboII GAAGA 1375 1374 . . 1370 1375 + CfrI YGGCCR 1370 1374 . . 1371 1374 + CviJI RGCY 1372 1372 . . 1371 1374 + HaeIII GGCC 1372 1372 . . 1381 1385 + TfiI GAWTC 1381 1384 . . 1381 1385 + HinfI GANTC 1381 1384 . . 1381 1391 + Hin4I GAYNNNNNVTC 1372 1367 1404 1399 1384 1388 + Hpy188I TCNGA 1386 1385 . . 1387 1391 + TfiI GAWTC 1387 1390 . . 1387 1391 + HinfI GANTC 1387 1390 . . 1390 1393 + TaqI TCGA 1390 1392 . . 1381 1391 - Hin4I GAYNNNNNVTC 1404 1399 1372 1367 1397 1400 + CviRI* TGCA 1398 1398 . . 1398 1402 + SfaNI GCATC 1407 1411 . . 1402 1405 + MnlI CCTC 1412 1411 . . 1399 1403 - FokI GGATG 1385 1389 . . 1399 1403 - BseGI GGATG 1398 1396 . . 1405 1409 + BssKI CCNGG 1404 1409 . . 1405 1409 + BseBI CCWGG 1406 1407 . . 1405 1410 + SecI* CCNNGG 1405 1409 . . 1405 1409 + ScrFI CCNGG 1406 1407 . . 1405 1409 + EcoRII CCWGG 1404 1409 . . 1403 1408 - Eco57MI CTGRAG 1388 1386 . . 1403 1408 - GsuI CTGGAG 1388 1386 . . 1410 1415 - BsmI GAATGC 1410 1408 . . 1415 1418 + AciI CCGC 1415 1417 . . 1432 1435 - MnlI CCTC 1425 1424 . . 1450 1458 + AlwNI CAGNNNCTG 1455 1452 . . 1451 1456 + HaeII RGCGCY 1455 1451 . . 1452 1455 + Hin6I GCGC 1452 1454 . . 1452 1455 + GlaI GCGC 1453 1453 . . 1452 1455 + HhaI GCGC 1454 1452 . . 1454 1459 + Cac8I GCNNGC 1456 1456 . . 1471 1476 + SduI GDGCHC 1475 1471 . . 1471 1476 + HgiJII* GRGCYC 1475 1471 . . 1472 1475 + CviJI RGCY 1473 1473 . . 1477 1480 + MseI TTAA 1477 1479 . . 1486 1491 + Hpy178III* TCNNGA 1487 1489 . . 1494 1500 + RsrII CGGWCCG 1495 1498 . . 1495 1499 + BmgT120I GGNCC 1496 1497 . . 1495 1499 + AvaII GGWCC 1495 1498 . . 1495 1499 + AsuI* GGNCC 1495 1498 . . 1493 1496 - AciI CCGC 1493 1495 . . 1498 1501 + AciI CCGC 1498 1500 . . 1500 1505 + Cac8I GCNNGC 1502 1502 . . 1501 1505 + FauI CCCGC 1509 1511 . . 1502 1505 + AciI CCGC 1502 1504 . . 1504 1514 + MwoI GCNNNNNNNGC 1510 1507 . . 1504 1514 + BstAPI GCANNNNNTGC 1510 1507 . . 1512 1515 + CviRI* TGCA 1513 1513 . . 1513 1517 + SfaNI GCATC 1522 1526 . . 1517 1520 + MnlI CCTC 1527 1526 . . 1517 1522 + SecI* CCNNGG 1517 1521 . . 1514 1518 - FokI GGATG 1500 1504 . . 1514 1518 - BseGI GGATG 1513 1511 . . 1519 1523 + Hpy188I TCNGA 1521 1520 . . 1521 1526 - BciVI GTATCC 1515 1514 . . 1531 1536 + Hpy166II GTNNAC 1533 1533 . . 1531 1536 + AccI GTMKAC 1532 1534 . . 1522 1532 - BaeI ACNNNNGTAYC 1547 1542 1514 1509 1547 1550 + MseI TTAA 1547 1549 . . 1551 1554 + TaqI TCGA 1551 1553 . . 1555 1558 + FatI CATG 1554 1558 . . 1555 1558 + NlaIII CATG 1558 1554 . . 1555 1558 + CviAII CATG 1555 1557 . . 1559 1563 + TspDTI ATGAA 1574 1572 . . 1561 1565 + MboII GAAGA 1573 1572 . . 1564 1568 + MboII GAAGA 1576 1575 . . 1567 1571 + TfiI GAWTC 1567 1570 . . 1567 1571 + HinfI GANTC 1567 1570 . . 1570 1576 + PfoI TCCNGGA 1570 1575 . . 1571 1575 + BssKI CCNGG 1570 1575 . . 1571 1575 + BseBI CCWGG 1572 1573 . . 1571 1575 + ScrFI CCNGG 1572 1573 . . 1571 1575 + EcoRII CCWGG 1570 1575 . . 1578 1582 + BsrI ACTGG 1583 1581 . . 1574 1579 - BciVI GTATCC 1568 1567 . . 1581 1586 + NlaIV GGNNCC 1583 1583 . . 1581 1585 + BmgT120I GGNCC 1582 1583 . . 1581 1585 + AvaII GGWCC 1581 1584 . . 1581 1585 + AsuI* GGNCC 1581 1584 . . 1584 1594 + BsiYI* CCNNNNNNNGG 1590 1587 . . 1611 1615 + TfiI GAWTC 1611 1614 . . 1611 1615 + HinfI GANTC 1611 1614 . . 1613 1617 - TspDTI ATGAA 1603 1601 . . 1624 1628 + TspDTI ATGAA 1639 1637 . . 1638 1642 + Hpy188I TCNGA 1640 1639 . . 1651 1655 + TspDTI ATGAA 1666 1664 . . 1661 1666 + ApoI RAATTY 1661 1665 . . 1662 1665 + TspEI AATT 1661 1665 . . 1667 1670 + TspEI AATT 1666 1670 . . 1672 1675 + SetI ASST 1675 1671 . . 1672 1675 + CviJI RGCY 1673 1673 . . 1672 1675 + AluI AGCT 1673 1673 . . 1674 1678 + DdeI CTNAG 1674 1677 . . 1680 1683 + CviJI RGCY 1681 1681 . . 1684 1689 + MfeI CAATTG 1684 1688 . . 1685 1688 + TspEI AATT 1684 1688 . . 1692 1697 + SspI AATATT 1694 1694 . . 1706 1709 + MseI TTAA 1706 1708 . . 1711 1715 - MboII GAAGA 1703 1702 . . 1717 1721 - TspDTI ATGAA 1707 1705 . . 1721 1724 + MseI TTAA 1721 1723 . . 1725 1728 + SetI ASST 1728 1724 . . 1730 1734 + SfaNI GCATC 1739 1743 . . 1739 1742 + MseI TTAA 1739 1741 . . 1767 1772 + ApoI RAATTY 1767 1771 . . 1768 1771 + TspEI AATT 1767 1771 . . 1785 1788 - MnlI CCTC 1778 1777 . . 1793 1797 - BceAI ACGGC 1778 1780 . . 1803 1813 + BaeI ACNNNNGTAYC 1792 1787 1825 1820 1805 1815 + FalI AAGNNNNNCTT 1796 1791 1828 1823 1809 1812 + Csp6I GTAC 1809 1811 . . 1809 1812 + RsaI GTAC 1810 1810 . . 1800 1810 - BaeI ACNNNNGTAYC 1825 1820 1792 1787 1811 1814 + SetI ASST 1814 1810 . . 1805 1815 - FalI AAGNNNNNCTT 1828 1823 1796 1791 1840 1843 + TaiI ACGT 1843 1839 . . 1840 1843 + SetI ASST 1843 1839 . . 1840 1843 + MaeII ACGT 1840 1842 . . 1842 1846 + MaeIII GTNAC 1841 1846 . . 1866 1871 + AhaIII* TTTAAA 1868 1868 . . 1867 1870 + MseI TTAA 1867 1869 . . 1879 1884 + AhaIII* TTTAAA 1881 1881 . . 1880 1883 + MseI TTAA 1880 1882 . . 1882 1887 + ApoI RAATTY 1882 1886 . . 1883 1886 + TspEI AATT 1882 1886 . . 1906 1911 + EcoRV GATATC 1908 1908 . . 1913 1916 + MseI TTAA 1913 1915 . . 1915 1918 + TspEI AATT 1914 1918 . . 1921 1924 + CviRI* TGCA 1922 1922 . . 1931 1934 + FatI CATG 1930 1934 . . 1931 1934 + NlaIII CATG 1934 1930 . . 1931 1934 + CviAII CATG 1931 1933 . . 1933 1936 + CviRI* TGCA 1934 1934 . . 1938 1943 + XhoII RGATCY 1938 1942 . . 1938 1943 + BglII AGATCT 1938 1942 . . 1939 1942 + DpnI GATC 1940 1940 . . 1939 1942 + BstKTI GATC 1941 1939 . . 1939 1942 + MboI GATC 1938 1942 . . 1941 1945 + Hpy188I TCNGA 1943 1942 . . 1944 1948 + TfiI GAWTC 1944 1947 . . 1944 1948 + HinfI GANTC 1944 1947 . . 1947 1951 + Hpy188I TCNGA 1949 1948 . . 1956 1961 + ApoI RAATTY 1956 1960 . . 1957 1960 + TspEI AATT 1956 1960 . . 1969 1979 + MwoI GCNNNNNNNGC 1975 1972 . . 1975 1978 + MaeI CTAG 1975 1977 . . 1977 1980 + SetI ASST 1980 1976 . . 1977 1980 + CviJI RGCY 1978 1978 . . 1977 1980 + AluI AGCT 1978 1978 . . 1980 1985 + BclI TGATCA 1980 1984 . . 1981 1984 + DpnI GATC 1982 1982 . . 1981 1984 + BstKTI GATC 1983 1981 . . 1981 1984 + MboI GATC 1980 1984 . . 1984 1989 + MfeI CAATTG 1984 1988 . . 1985 1988 + TspEI AATT 1984 1988 . . 1988 1991 + CviRI* TGCA 1989 1989 . . 1991 1994 + TspEI AATT 1990 1994 . . 1993 1996 + MseI TTAA 1993 1995 . . 2000 2005 + ApoI RAATTY 2000 2004 . . 2001 2004 + TspEI AATT 2000 2004 . . 2006 2011 + NdeI CATATG 2007 2009 . . 2004 2008 - TspDTI ATGAA 1994 1992 . . 2011 2021 + Hin4I GAYNNNNNVTC 2002 1997 2034 2029 2024 2029 + StuI AGGCCT 2026 2026 . . 2025 2028 + CviJI RGCY 2026 2026 . . 2025 2028 + HaeIII GGCC 2026 2026 . . 2027 2031 + Hin4II* CCTTC 2037 2036 . . 2034 2039 + SspI AATATT 2036 2036 . . 2041 2045 + MboII GAAGA 2053 2052 . . 2044 2048 + PleI GAGTC 2052 2053 . . 2044 2048 + MlyI GAGTC 2053 2053 . . 2044 2048 + HinfI GANTC 2044 2047 . . 2041 2046 - Ksp632I* CTCTTC 2036 2039 . . 2046 2050 + BsmAI GTCTC 2051 2055 . . 2050 2054 + DdeI CTNAG 2050 2053 . . 2056 2060 + TfiI GAWTC 2056 2059 . . 2056 2060 + HinfI GANTC 2056 2059 . . 2064 2068 + HinfI GANTC 2064 2067 . . 2066 2070 + DdeI CTNAG 2066 2069 . . 2066 2070 + BspCNI CTCAG 2079 2077 . . 2066 2070 + BseMII CTCAG 2080 2078 . . 2064 2068 - PleI GAGTC 2058 2059 . . 2064 2068 - MlyI GAGTC 2058 2058 . . 2070 2074 + MaeIII GTNAC 2069 2074 . . 2076 2079 + CviJI RGCY 2077 2077 . . 2080 2083 + MseI TTAA 2080 2082 . . 2093 2097 + DdeI CTNAG 2093 2096 . . 2093 2097 + BspCNI CTCAG 2106 2104 . . 2093 2097 + BseMII CTCAG 2107 2105 . . 2114 2117 + DpnI GATC 2115 2115 . . 2114 2117 + BstKTI GATC 2116 2114 . . 2114 2117 + MboI GATC 2113 2117 . . 2129 2134 + HindIII AAGCTT 2129 2133 . . 2130 2133 + SetI ASST 2133 2129 . . 2130 2133 + CviJI RGCY 2131 2131 . . 2130 2133 + AluI AGCT 2131 2131 . . 2140 2144 + TfiI GAWTC 2140 2143 . . 2140 2144 + HinfI GANTC 2140 2143 . . 2153 2157 + Hpy188I TCNGA 2155 2154 . . 2156 2166 + Hin4I GAYNNNNNVTC 2147 2142 2179 2174 2158 2161 + CviRI* TGCA 2159 2159 . . 2156 2160 - SfaNI GCATC 2146 2150 . . 2166 2171 + DsaI* CCRYGG 2166 2170 . . 2166 2171 + StyI CCWWGG 2166 2170 . . 2166 2171 + NcoI CCATGG 2166 2170 . . 2166 2171 + SecI* CCNNGG 2166 2170 . . 2167 2170 + FatI CATG 2166 2170 . . 2167 2170 + NlaIII CATG 2170 2166 . . 2167 2170 + CviAII CATG 2167 2169 . . 2170 2175 + XhoII RGATCY 2170 2174 . . 2170 2175 + NlaIV GGNNCC 2172 2172 . . 2170 2175 + BamHI GGATCC 2170 2174 . . 2170 2174 + BinI* GGATC 2178 2179 . . 2171 2174 + DpnI GATC 2172 2172 . . 2171 2174 + BstKTI GATC 2173 2171 . . 2171 2174 + MboI GATC 2170 2174 . . 2171 2175 - BinI* GGATC 2165 2166 . . 2177 2180 + TspEI AATT 2176 2180 . . 2182 2185 + MseI TTAA 2182 2184 . . 2194 2203 + PshAI GACNNNNGTC 2198 2198 . . 2222 2232 + BsiYI* CCNNNNNNNGG 2228 2225 . . 2225 2228 + SetI ASST 2228 2224 . . 2226 2229 + MnlI CCTC 2236 2235 . . 2226 2236 + BsiYI* CCNNNNNNNGG 2232 2229 . . 2239 2243 + TspGWI ACGGA 2254 2252 . . 2243 2248 + ApoI RAATTY 2243 2247 . . 2244 2247 + TspEI AATT 2243 2247 . . 2251 2256 + SspI AATATT 2253 2253 . . 2257 2261 + HphI GGTGA 2269 2268 . . 2260 2263 - MnlI CCTC 2253 2252 . . 2263 2267 + MboII GAAGA 2275 2274 . . 2268 2271 + SetI ASST 2271 2267 . . 2266 2270 - Hin4II* CCTTC 2260 2259 . . 2286 2291 + SfeI* CTRYAG 2286 2290 . . 2297 2302 + Hpy166II GTNNAC 2299 2299 . . 2293 2297 - BccI CCATC 2287 2288 . . 2315 2320 + Hpy166II GTNNAC 2317 2317 . . 2315 2320 + AccI GTMKAC 2316 2318 . . 2323 2326 + SetI ASST 2326 2322 . . 2324 2329 + Eco31I GGTCTC 2330 2334 . . 2325 2329 + BsmAI GTCTC 2330 2334 . . 2331 2341 + BsiYI* CCNNNNNNNGG 2337 2334 . . 2330 2334 - TspGWI ACGGA 2320 2318 . . 2337 2342 + EciI GGCGGA 2353 2351 . . 2341 2345 + TfiI GAWTC 2341 2344 . . 2341 2345 + HinfI GANTC 2341 2344 . . 2338 2341 - AciI CCGC 2338 2340 . . 2372 2375 + SetI ASST 2375 2371 . . 2372 2375 + CviJI RGCY 2373 2373 . . 2372 2375 + AluI AGCT 2373 2373 . . 2381 2384 + TaqI TCGA 2381 2383 . . 2385 2390 + SspI AATATT 2387 2387 . . 2389 2392 + MseI TTAA 2389 2391 . . 2400 2403 + MnlI CCTC 2410 2409 . . 2396 2400 - SfaNI GCATC 2386 2390 . . 2416 2425 + MslI CAYNNNNRTG 2420 2420 . . 2421 2425 + FokI GGATG 2434 2438 . . 2421 2425 + BseGI GGATG 2427 2425 . . 2425 2429 + PleI GAGTC 2433 2434 . . 2425 2429 + MlyI GAGTC 2434 2434 . . 2425 2429 + HinfI GANTC 2425 2428 . . 2428 2432 + Hpy188I TCNGA 2430 2429 . . 2442 2447 + ClaI ATCGAT 2443 2445 . . 2443 2446 + TaqI TCGA 2443 2445 . . 2463 2467 + Hpy188I TCNGA 2465 2464 . . 2466 2469 + DpnI GATC 2467 2467 . . 2466 2469 + BstKTI GATC 2468 2466 . . 2466 2469 + MboI GATC 2465 2469 . . 2468 2471 + TaqI TCGA 2468 2470 . . 2488 2491 + MseI TTAA 2488 2490 . . 2503 2506 + MaeI CTAG 2503 2505 . . 2506 2510 + MboII GAAGA 2518 2517 . . 2513 2517 + Tsp4CI* ACNGT 2515 2514 . . 2514 2520 + TspRI CASTGNN 2520 2511 . . 2519 2522 + MnlI CCTC 2529 2528 . . 2541 2547 + TspRI CASTGNN 2547 2538 . . 2549 2552 + CviRI* TGCA 2550 2550 . . 2557 2562 + BetI* WCCGGW 2557 2561 . . 2558 2561 + HpaII CCGG 2558 2560 . . 2555 2559 - HphI GGTGA 2547 2546 . . 2574 2578 - Hin4II* CCTTC 2568 2567 . . 2578 2583 + Cac8I GCNNGC 2580 2580 . . 2593 2603 + Hin4I GAYNNNNNVTC 2584 2579 2616 2611 2599 2602 + CviJI RGCY 2600 2600 . . 2596 2600 - BccI CCATC 2590 2591 . . 2614 2624 + Hin4I GAYNNNNNVTC 2605 2600 2637 2632 2620 2624 + TfiI GAWTC 2620 2623 . . 2620 2624 + HinfI GANTC 2620 2623 . . 2614 2624 - Hin4I GAYNNNNNVTC 2637 2632 2605 2600 2638 2642 + BinI* GGATC 2646 2647 . . 2639 2642 + DpnI GATC 2640 2640 . . 2639 2642 + BstKTI GATC 2641 2639 . . 2639 2642 + MboI GATC 2638 2642 . . 2641 2645 + Hpy188I TCNGA 2643 2642 . . 2643 2647 + FokI GGATG 2656 2660 . . 2643 2647 + BseGI GGATG 2649 2647 . . 2648 2651 + Csp6I GTAC 2648 2650 . . 2644 2648 - BccI CCATC 2638 2639 . . 2648 2651 + RsaI GTAC 2649 2649 . . 2649 2654 + SnaBI TACGTA 2651 2651 . . 2649 2654 + BsaAI YACGTR 2651 2651 . . 2650 2653 + TaiI ACGT 2653 2649 . . 2650 2653 + SetI ASST 2653 2649 . . 2650 2653 + MaeII ACGT 2650 2652 . . 2660 2663 + TspEI AATT 2659 2663 . . 2662 2665 + MseI TTAA 2662 2664 . . 2670 2674 + Hpy188I TCNGA 2672 2671 . . 2673 2685 + AloI GAACNNNNNNTCC 2665 2660 2697 2692 2675 2679 + TspGWI ACGGA 2690 2688 . . 2677 2682 + XhoII RGATCY 2677 2681 . . 2677 2681 + BinI* GGATC 2685 2686 . . 2678 2681 + DpnI GATC 2679 2679 . . 2678 2681 + BstKTI GATC 2680 2678 . . 2678 2681 + MboI GATC 2677 2681 . . 2680 2684 - MboII GAAGA 2672 2671 . . 2695 2698 + FnuDII* CGCG 2696 2696 . . 2699 2703 + PleI GAGTC 2707 2708 . . 2699 2703 + MlyI GAGTC 2708 2708 . . 2699 2703 + HinfI GANTC 2699 2702 . . 2696 2699 - AciI CCGC 2696 2698 . . 2718 2721 + SetI ASST 2721 2717 . . 2714 2719 - Eco57MI CTGRAG 2699 2697 . . 2714 2719 - Eco57I CTGAAG 2699 2697 . . 2726 2729 + TspEI AATT 2725 2729 . . 2732 2735 + CviRI* TGCA 2733 2733 . . 2731 2736 - BsgI GTGCAG 2716 2714 . . 2740 2745 + Eco57MI CTGRAG 2761 2759 . . 2740 2745 + Eco57I CTGAAG 2761 2759 . . 2766 2769 + TaqI TCGA 2766 2768 . . 2767 2771 + Hpy99I CGWCG 2771 2766 . . 2768 2772 + HgaI GACGC 2777 2782 . . 2780 2785 + TatI WGTACW 2780 2784 . . 2781 2784 + Csp6I GTAC 2781 2783 . . 2781 2784 + RsaI GTAC 2782 2782 . . 2803 2808 + Hpy166II GTNNAC 2805 2805 . . 2803 2808 + HindII GTYRAC 2805 2805 . . 2808 2813 + McrI* CGRYCG 2811 2809 . . 2808 2813 + PvuI CGATCG 2811 2809 . . 2809 2812 + DpnI GATC 2810 2810 . . 2809 2812 + BstKTI GATC 2811 2809 . . 2809 2812 + MboI GATC 2808 2812 . . #--------------------------------------- # # # AatII: # [GACGTC] # 737 2098 # # Acc65I: # [GGTACC] # 285 2550 # # AccI: # [GTMKAC] # 224 519 784 1308 # # AciI: # [CCGC] # 4 5 10 43 78 139 # 358 836 1362 # # AcyI: # [GRCGYC] # 734 2101 # # AflIII: # [ACRYGT] # 361 571 1903 # # AgeI: # [ACCGGT] # 65 209 2561 # # AhaIII*: # [TTTAAA] # 13 954 1868 # # AloI: # [GAACNNNNNNTCC] # 32 138 2665 # # AluI: # [AGCT] # 76 153 242 247 265 305 # 462 485 600 # # AlwNI: # [CAGNNNCTG] # 1380 1455 # # ApoI: # [RAATTY] # 44 74 106 115 243 592 # 593 1068 # # AsuI*: # [GGNCC] # 86 227 317 951 1254 # # AsuII: # [TTCGAA] # 1231 1604 # # AvaII: # [GGWCC] # 86 317 1178 1254 # # BaeI: # [ACNNNNGTAYC] # 0 0 33 33 245 1010 # 1514 # # BamHI: # [GGATCC] # 665 2170 # # BarI: # [GAAGNNNNNNTAC] # 32 145 2658 # # BbvI: # [GCAGC] # 57 518 2260 # # BccI: # [CCATC] # 48 197 233 303 2054 # # BceAI: # [ACGGC] # 1057 1778 # # BciVI: # [GTATCC] # 51 53 284 457 723 1267 # # BclI: # [TGATCA] # 305 855 1675 # # BdaI: # [TGANNNNNNTCA] # 0 0 0 0 34 34 # 202 247 2318 # # BetI*: # [WCCGGW] # 65 182 209 278 2101 # # BfiI: # [ACTGGG] # 1257 1578 # # BglII: # [AGATCT] # 897 954 984 # # BinI*: # [GGATC] # 13 39 150 426 460 468 # 1279 # # BisI: # [GCNGC] # 46 543 2246 # # BlsI: # [GCNGC] # 47 543 2245 # # BmgT120I: # [GGNCC] # 86 227 318 951 1253 # # BsaAI: # [YACGTR] # 184 209 365 2077 # # BsaXI: # [ACNNNNNCTCC] # 30 889 1916 # # BseBI: # [CCWGG] # 18 118 166 250 291 729 # 1263 # # BseGI: # [GGATG] # 115 115 186 222 225 914 # 1058 # # BseMII: # [CTCAG] # 27 728 2080 # # BsgI: # [GTGCAG] # 119 2716 # # BsiYI*: # [CCNNNNNNNGG] # 4 105 321 406 498 638 # 863 # # BslFI: # [GGGAC] # 330 634 1871 # # BsmAI: # [GTCTC] # 279 505 611 1440 # # BsmI: # [GAATGC] # 492 918 1425 # # BspCNI: # [CTCAG] # 27 729 2079 # # BspHI: # [TCATGA] # 972 1863 # # BspMII*: # [TCCGGA] # 456 2379 # # BsrI: # [ACTGG] # 41 320 1222 1252 # # BssKI: # [CCNGG] # 18 118 166 250 289 729 # 1265 # # BstAPI: # [GCANNNNNTGC] # 1325 1510 # # BstKTI: # [GATC] # 24 32 39 39 42 56 # 57 93 131 133 160 173 # 220 295 387 954 # # BstXI: # [CCANNNNNNTGG] # 681 2154 # # BtrI: # [CACGTC] # 933 1902 # # BtsI: # [GCAGTG] # 1202 1633 # # Cac8I: # [GCNNGC] # 46 255 1078 1456 # # Cfr10I: # [RCCGGY] # 65 209 2561 # # CfrI: # [YGGCCR] # 1370 1465 # # ClaI: # [ATCGAT] # 392 855 1588 # # Csp6I: # [GTAC] # 54 76 133 210 325 330 # 839 868 # # CviAII: # [CATG] # 236 376 582 668 973 # # CviJI: # [RGCY] # 8 18 29 42 47 48 # 51 54 81 85 101 200 # 223 227 235 242 247 297 # 600 # # CviRI*: # [TGCA] # 12 12 55 102 102 115 # 170 183 198 201 391 393 # 409 492 # # DdeI: # [CTNAG] # 16 27 243 376 571 742 # 860 # # DpnI: # [GATC] # 25 31 39 39 42 56 # 57 93 131 133 160 173 # 220 295 387 954 # # DraIII: # [CACNNNGTG] # 553 2282 # # DsaI*: # [CCRYGG] # 669 2166 # # Eam1105I: # [GACNNNNNGTC] # 468 2367 # # EciI: # [GGCGGA] # 482 2353 # # Eco31I: # [GGTCTC] # 505 611 1719 # # Eco57I: # [CTGAAG] # 62 74 1168 1531 # # Eco57MI: # [CTGRAG] # 62 74 220 1168 1311 # # EcoRII: # [CCWGG] # 18 118 166 250 289 729 # 1265 # # EcoRV: # [GATATC] # 927 1908 # # FalI: # [AAGNNNNNCTT] # 0 0 0 0 0 0 # 0 0 0 0 25 32 # 32 32 32 32 62 184 # 343 1007 1054 # # FatI: # [CATG] # 236 376 582 669 972 # # FauI: # [CCCGC] # 1326 1509 # # FnuDII*: # [CGCG] # 139 2696 # # FokI: # [GGATG] # 115 115 179 212 222 934 # 1058 # # GlaI: # [GCGC] # 245 344 864 1382 # # GsuI: # [CTGGAG] # 1388 1447 # # HaeII: # [RGCGCY] # 346 1109 1380 # # HaeIII: # [GGCC] # 546 654 809 826 # # HgaI: # [GACGC] # 58 2777 # # HgiCI*: # [GGYRCC] # 285 2550 # # HgiJII*: # [GRGCYC] # 1360 1475 # # HhaI: # [GCGC] # 245 345 864 1381 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 0 0 # 0 0 0 0 11 21 # 21 32 32 32 32 32 # 32 32 50 113 113 198 # 211 309 405 561 598 # # Hin4II*: # [CCTTC] # 223 267 308 345 1692 # # Hin6I: # [GCGC] # 245 343 864 1383 # # HindII: # [GTYRAC] # 30 122 2683 # # HindIII: # [AAGCTT] # 706 2129 # # HinfI: # [GANTC] # 6 8 12 13 44 76 # 79 81 84 93 100 136 # 166 180 195 201 246 333 # 782 # # HpaII: # [CCGG] # 66 182 209 277 2101 # # HphI: # [GGTGA] # 8 221 278 288 308 674 # 1058 # # Hpy166II: # [GTNNAC] # 18 30 122 129 224 488 # 766 1058 # # Hpy178III*: # [TCNNGA] # 46 50 197 317 420 457 # 1348 # # Hpy188I: # [TCNGA] # 6 22 29 35 119 135 # 163 178 206 246 275 303 # 1118 # # Hpy99I: # [CGWCG] # 64 474 2297 # # KpnI: # [GGTACC] # 289 2546 # # Ksp632I*: # [CTCTTC] # 799 2036 # # MaeI: # [CTAG] # 332 399 528 613 963 # # MaeII: # [ACGT] # 11 45 73 116 185 198 # 208 292 810 897 # # MaeIII: # [GTNAC] # 228 277 354 593 617 766 # # MboI: # [GATC] # 27 29 39 39 42 56 # 57 93 131 133 160 173 # 220 295 387 954 # # MboII: # [GAAGA] # 3 127 154 163 165 198 # 222 243 350 496 714 # # McrI*: # [CGRYCG] # 24 32 823 1956 # # MfeI: # [CAATTG] # 300 851 1684 # # MlyI: # [GAGTC] # 5 7 127 174 274 376 # 909 963 # # MmeI: # [TCCRAC] # 863 1972 # # MnlI: # [CCTC] # 13 16 17 36 53 98 # 102 104 119 157 161 184 # 209 223 251 306 328 458 # # MseI: # [TTAA] # 13 15 18 33 70 80 # 87 90 99 102 115 128 # 132 159 173 174 174 207 # 222 361 383 # # MslI: # [CAYNNNNRTG] # 415 508 837 1075 # # MwoI: # [GCNNNNNNNGC] # 465 860 1510 # # NcoI: # [CCATGG] # 669 2166 # # NdeI: # [CATATG] # 828 2007 # # NlaIII: # [CATG] # 236 376 582 665 976 # # NlaIV: # [GGNNCC] # 31 227 287 589 663 1038 # # PfoI: # [TCCNGGA] # 1265 1570 # # PleI: # [GAGTC] # 6 7 128 174 274 375 # 909 962 # # PmaCI: # [CACGTG] # 574 2261 # # PshAI: # [GACNNNNGTC] # 637 2198 # # PsrI: # [GAACNNNNNNTAC] # 32 677 2126 # # PvuI: # [CGATCG] # 24 32 823 1956 # # RsaI: # [GTAC] # 53 76 133 211 325 330 # 839 868 # # RsrII: # [CGGWCCG] # 1340 1495 # # ScrFI: # [CCNGG] # 18 118 166 250 291 729 # 1263 # # SduI: # [GDGCHC] # 1360 1475 # # SecI*: # [CCNNGG] # 112 236 251 289 629 649 # 669 # # SetI: # [ASST] # 4 5 11 14 28 29 # 31 34 35 41 41 43 # 49 53 55 68 86 86 # 95 114 116 129 137 153 # 165 211 244 278 480 # # SfaNI: # [GCATC] # 115 217 240 407 449 1407 # # SfeI*: # [CTRYAG] # 549 2286 # # SgfI: # [GCGATCGC] # 32 2803 # # SnaBI: # [TACGTA] # 184 209 2442 # # SpeI: # [ACTAGT] # 612 2223 # # SspI: # [AATATT] # 134 217 342 448 569 1125 # # StuI: # [AGGCCT] # 809 2026 # # StyI: # [CCWWGG] # 669 776 1390 # # TaiI: # [ACGT] # 11 45 73 116 182 198 # 211 292 810 897 # # TaqI: # [TCGA] # 4 25 28 33 62 69 # 159 161 185 298 317 321 # 343 830 # # TaqII: # [GACCGA] # 139 374 2322 # # TatI: # [WGTACW] # 55 2780 # # TfiI: # [GAWTC] # 6 44 84 94 112 180 # 201 215 279 333 412 875 # # TseI: # [GCWGC] # 45 543 2247 # # Tsp4CI*: # [ACNGT] # 38 82 312 320 569 1514 # # TspDTI: # [ATGAA] # 16 18 27 29 36 41 # 76 139 231 277 287 354 # 463 841 # # TspEI: # [AATT] # 5 6 9 10 14 18 # 19 28 32 36 42 49 # 66 67 79 83 95 110 # 115 127 168 176 321 330 # 414 416 # # TspGWI: # [ACGGA] # 66 145 370 458 1796 # # TspRI: # [CASTGNN] # 27 61 288 1209 1250 # # VspI: # [ATTAAT] # 1255 1580 # # XhoII: # [RGATCY] # 158 232 507 954 984 # # XmnI: # [GAANNNNTTC] # 47 452 2336 # # ZraI: # [GACGTC] # 735 2100 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 2835 # Reported_sequences: 1 # Reported_hitcount: 649 #---------------------------------------