Restriction Fragments for PRY3/YJL078C



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The currently selected gene/sequence is :

PRY3/YJL078C on chromosome X from coordinates 293981 to 291336.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.10750.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 4 9 + Eco57MI CTGRAG 25 23 . . 4 9 + GsuI CTGGAG 25 23 . . 28 31 + MaeI CTAG 28 30 . . 31 35 + FokI GGATG 44 48 . . 31 35 + BseGI GGATG 37 35 . . 32 36 - SfaNI GCATC 22 26 . . 37 40 + MaeI CTAG 37 39 . . 42 45 + CviJI RGCY 43 43 . . 43 53 + MwoI GCNNNNNNNGC 49 46 . . 43 53 + BglI GCCNNNNNGGC 49 46 . . 43 47 - BceAI ACGGC 28 30 . . 61 64 + TaiI ACGT 64 60 . . 61 64 + SetI ASST 64 60 . . 61 64 + MaeII ACGT 61 63 . . 74 77 + TaqI TCGA 74 76 . . 74 79 + Hpy178III* TCNNGA 75 77 . . 80 85 + BtgZI GCGATG 95 99 . . 87 97 + MwoI GCNNNNNNNGC 93 90 . . 107 111 + Hpy188I TCNGA 109 108 . . 111 116 + HaeII RGCGCY 115 111 . . 111 116 + Eco47III AGCGCT 113 113 . . 112 115 + Hin6I GCGC 112 114 . . 112 115 + GlaI GCGC 113 113 . . 112 115 + HhaI GCGC 114 112 . . 117 122 + BspLU11I* ACATGT 117 121 . . 117 122 + NspI RCATGY 121 117 . . 117 122 + AflIII ACRYGT 117 121 . . 118 121 + FatI CATG 117 121 . . 118 121 + NlaIII CATG 121 117 . . 118 121 + CviAII CATG 118 120 . . 121 126 + Hpy166II GTNNAC 123 123 . . 121 126 + HindII GTYRAC 123 123 . . 129 134 + HaeII RGCGCY 133 129 . . 130 133 + Hin6I GCGC 130 132 . . 130 133 + GlaI GCGC 131 131 . . 130 133 + HhaI GCGC 132 130 . . 132 136 + TauI GCSGC 135 132 . . 132 136 + BlsI GCNGC 134 133 . . 132 136 + BisI GCNGC 133 134 . . 133 136 + AciI CCGC 133 135 . . 133 138 + BsrBI CCGCTC 135 135 . . 139 142 + SetI ASST 142 138 . . 139 145 + SexAI ACCWGGT 139 144 . . 140 144 + BssKI CCNGG 139 144 . . 140 144 + BseBI CCWGG 141 142 . . 140 144 + ScrFI CCNGG 141 142 . . 140 144 + EcoRII CCWGG 139 144 . . 137 141 - HphI GGTGA 129 128 . . 143 147 + BmgT120I GGNCC 144 145 . . 143 147 + AvaII GGWCC 143 146 . . 143 147 + AsuI* GGNCC 143 146 . . 145 150 + MmeI TCCRAC 170 168 . . 145 149 + Hpy188I TCNGA 147 146 . . 155 160 + BalI TGGCCA 157 157 . . 155 160 + CfrI YGGCCR 155 159 . . 156 159 + CviJI RGCY 157 157 . . 156 159 + HaeIII GGCC 157 157 . . 157 167 + MwoI GCNNNNNNNGC 163 160 . . 160 163 + SetI ASST 163 159 . . 165 170 + MstI* TGCGCA 167 167 . . 166 169 + Hin6I GCGC 166 168 . . 166 169 + GlaI GCGC 167 167 . . 166 169 + HhaI GCGC 168 166 . . 205 208 + MseI TTAA 205 207 . . 211 220 + MslI CAYNNNNRTG 215 215 . . 214 218 + Hpy188I TCNGA 216 215 . . 210 215 - BsmI GAATGC 210 208 . . 220 223 + CviJI RGCY 221 221 . . 220 223 + HaeIII GGCC 221 221 . . 220 224 + BmgT120I GGNCC 221 222 . . 220 224 + AsuI* GGNCC 220 223 . . 217 221 - BccI CCATC 211 212 . . 224 229 + NdeI CATATG 225 227 . . 229 233 + HphI GGTGA 241 240 . . 236 239 + SetI ASST 239 235 . . 243 248 + StyI CCWWGG 243 247 . . 243 248 + SecI* CCNNGG 243 247 . . 248 252 + MaeIII GTNAC 247 252 . . 246 251 - TsoI TARCCA 236 234 . . 265 275 + MwoI GCNNNNNNNGC 271 268 . . 268 273 + Hpy166II GTNNAC 270 270 . . 265 268 - AciI CCGC 265 267 . . 263 268 - BsrBI CCGCTC 265 265 . . 271 275 + HgaI GACGC 280 285 . . 271 276 + AcyI GRCGYC 272 274 . . 275 279 + BssKI CCNGG 274 279 . . 275 279 + BseBI CCWGG 276 277 . . 275 279 + ScrFI CCNGG 276 277 . . 275 279 + EcoRII CCWGG 274 279 . . 275 285 + BsiYI* CCNNNNNNNGG 281 278 . . 279 282 + Csp6I GTAC 279 281 . . 279 282 + RsaI GTAC 280 280 . . 301 304 + TspEI AATT 300 304 . . 312 318 + PfoI TCCNGGA 312 317 . . 313 317 + BssKI CCNGG 312 317 . . 313 317 + ScrFI CCNGG 314 315 . . 313 317 + CauII* CCSGG 314 315 . . 314 317 + HpaII CCGG 314 316 . . 322 326 + Hpy188I TCNGA 324 323 . . 325 329 + TfiI GAWTC 325 328 . . 325 329 + HinfI GANTC 325 328 . . 329 334 + DsaI* CCRYGG 329 333 . . 329 334 + SecI* CCNNGG 329 333 . . 335 339 + Tsp45I GTSAC 334 339 . . 335 339 + MaeIII GTNAC 334 339 . . 342 351 + MslI CAYNNNNRTG 346 346 . . 342 351 + OliI CACNNNNGTG 346 346 . . 347 350 + SetI ASST 350 346 . . 360 365 + Hpy166II GTNNAC 362 362 . . 360 365 + HindII GTYRAC 362 362 . . 365 368 + AciI CCGC 365 367 . . 367 372 + NmeAIII GCCGAG 393 391 . . 376 380 + FokI GGATG 389 393 . . 376 380 + BseGI GGATG 382 380 . . 384 389 + PsiI TTATAA 386 386 . . 381 386 - TsoI TARCCA 371 369 . . 389 394 + SspI AATATT 391 391 . . 398 401 + Csp6I GTAC 398 400 . . 398 401 + RsaI GTAC 399 399 . . 404 407 + FatI CATG 403 407 . . 404 407 + NlaIII CATG 407 403 . . 404 407 + CviAII CATG 404 406 . . 412 415 + TspEI AATT 411 415 . . 423 428 + SduI GDGCHC 427 423 . . 423 428 + HgiAI* GWGCWC 427 423 . . 436 439 + MnlI CCTC 446 445 . . 438 444 + PfoI TCCNGGA 438 443 . . 439 443 + BssKI CCNGG 438 443 . . 439 443 + BseBI CCWGG 440 441 . . 439 443 + ScrFI CCNGG 440 441 . . 439 443 + EcoRII CCWGG 438 443 . . 449 452 + SetI ASST 452 448 . . 450 454 + BssKI CCNGG 449 454 . . 450 454 + BseBI CCWGG 451 452 . . 450 455 + SecI* CCNNGG 450 454 . . 450 454 + ScrFI CCNGG 451 452 . . 450 454 + EcoRII CCWGG 449 454 . . 454 458 + HphI GGTGA 466 465 . . 452 457 - TaqII CACCCA 442 440 . . 462 465 + CviRI* TGCA 463 463 . . 466 469 - MnlI CCTC 459 458 . . 474 479 + NlaIV GGNNCC 476 476 . . 482 487 + PsiI TTATAA 484 484 . . 489 495 + TspRI CASTGNN 495 486 . . 490 494 + Tsp4CI* ACNGT 492 491 . . 497 500 + MnlI CCTC 507 506 . . 503 506 + MnlI CCTC 513 512 . . 509 512 + MnlI CCTC 519 518 . . 506 510 - FokI GGATG 492 496 . . 506 510 - BseGI GGATG 505 503 . . 515 518 + MnlI CCTC 525 524 . . 516 521 + Ksp632I* CTCTTC 522 525 . . 517 521 - MboII GAAGA 509 508 . . 523 526 + SetI ASST 526 522 . . 524 527 + MnlI CCTC 534 533 . . 535 539 + Hpy188I TCNGA 537 536 . . 538 548 + Hin4I GAYNNNNNVTC 529 524 561 556 538 546 + Tth111I GACNNNGTC 541 542 . . 539 549 + BsaXI ACNNNNNCTCC 529 526 559 556 541 545 + Tsp4CI* ACNGT 543 542 . . 544 548 + BsmAI GTCTC 549 553 . . 551 555 + BccI CCATC 559 560 . . 557 561 - FokI GGATG 543 547 . . 557 561 - BseGI GGATG 556 554 . . 560 564 - BsrI ACTGG 560 558 . . 570 575 + Cac8I GCNNGC 572 572 . . 571 575 + FauI CCCGC 579 581 . . 572 577 + NspBII* CMGCKG 574 574 . . 572 575 + AciI CCGC 572 574 . . 575 580 + SfeI* CTRYAG 575 579 . . 580 583 + Hin6I GCGC 580 582 . . 580 583 + GlaI GCGC 581 581 . . 580 583 + HhaI GCGC 582 580 . . 588 593 + Hpy166II GTNNAC 590 590 . . 588 593 + BssNAI GTATAC 590 590 . . 588 593 + AccI GTMKAC 589 591 . . 595 605 + BaeI ACNNNNGTAYC 584 579 617 612 598 602 + Tsp4CI* ACNGT 600 599 . . 610 614 + TauI GCSGC 613 610 . . 610 614 + BlsI GCNGC 612 611 . . 610 614 + BisI GCNGC 611 612 . . 612 615 + CviJI RGCY 613 613 . . 613 618 + Cac8I GCNNGC 615 615 . . 613 618 + BmtI GCTAGC 617 613 . . 613 618 + NheI GCTAGC 613 617 . . 610 613 - AciI CCGC 610 612 . . 614 617 + MaeI CTAG 614 616 . . 617 621 + BlsI GCNGC 619 618 . . 617 621 + BisI GCNGC 618 619 . . 617 621 + BbvI GCAGC 629 633 . . 617 621 + TseI GCWGC 617 620 . . 618 623 + EcoP15I CAGCAG 648 650 . . 627 632 + AhaIII* TTTAAA 629 629 . . 628 631 + MseI TTAA 628 630 . . 634 637 + TaqI TCGA 634 636 . . 635 639 + Hpy99I CGWCG 639 634 . . 652 662 + MwoI GCNNNNNNNGC 658 655 . . 659 662 + AciI CCGC 659 661 . . 666 669 + MnlI CCTC 676 675 . . 669 675 + TspRI CASTGNN 675 666 . . 669 674 - BtsI GCAGTG 668 666 . . 677 680 + MnlI CCTC 687 686 . . 673 677 - HgaI GACGC 662 667 . . 678 683 + Ksp632I* CTCTTC 684 687 . . 679 683 - MboII GAAGA 671 670 . . 685 689 + Tsp4CI* ACNGT 687 686 . . 690 693 + TspEI AATT 689 693 . . 694 699 + SpeI ACTAGT 694 698 . . 695 698 + MaeI CTAG 695 697 . . 706 710 + TfiI GAWTC 706 709 . . 706 710 + HinfI GANTC 706 709 . . 715 720 + XhoII RGATCY 715 719 . . 715 720 + NlaIV GGNNCC 717 717 . . 715 720 + BamHI GGATCC 715 719 . . 715 719 + BinI* GGATC 723 724 . . 716 719 + DpnI GATC 717 717 . . 716 719 + BstKTI GATC 718 716 . . 716 719 + MboI GATC 715 719 . . 712 717 - MmeI TCCRAC 693 691 . . 719 722 + MnlI CCTC 729 728 . . 716 720 - BinI* GGATC 710 711 . . 723 729 + TspRI CASTGNN 729 720 . . 724 728 + Tsp4CI* ACNGT 726 725 . . 727 731 + BsmAI GTCTC 732 736 . . 735 738 + CviJI RGCY 736 736 . . 737 740 + MnlI CCTC 747 746 . . 738 743 + SmlI CTYRAG 738 742 . . 742 745 + SetI ASST 745 741 . . 742 745 + CviJI RGCY 743 743 . . 742 745 + AluI AGCT 743 743 . . 743 749 + SapI GCTCTTC 750 753 . . 738 743 - Bce83I* CTTGAG 723 721 . . 744 749 + Ksp632I* CTCTTC 750 753 . . 745 749 - MboII GAAGA 737 736 . . 751 755 + Tsp45I GTSAC 750 755 . . 751 755 + MaeIII GTNAC 750 755 . . 769 772 + SetI ASST 772 768 . . 770 773 + MnlI CCTC 780 779 . . 773 780 + PspXI VCTCGAGB 774 778 . . 773 776 + MnlI CCTC 783 782 . . 774 779 + SmlI CTYRAG 774 778 . . 774 779 + XhoI CTCGAG 774 778 . . 774 779 + BmeT110I CYCGRG 775 777 . . 774 779 + AvaI CYCGRG 774 778 . . 775 778 + TaqI TCGA 775 777 . . 771 776 - BseRI GAGGAG 762 760 . . 779 782 + Csp6I GTAC 779 781 . . 779 782 + RsaI GTAC 780 780 . . 781 785 + Tsp4CI* ACNGT 783 782 . . 783 787 + Hpy99I CGWCG 787 782 . . 786 791 + Esp3I CGTCTC 792 796 . . 787 791 + BsmAI GTCTC 792 796 . . 791 794 + MaeI CTAG 791 793 . . 796 800 - SfaNI GCATC 786 790 . . 804 808 - TspDTI ATGAA 794 792 . . 806 810 - FokI GGATG 792 796 . . 806 810 - BseGI GGATG 805 803 . . 820 823 + SetI ASST 823 819 . . 821 824 + MnlI CCTC 831 830 . . 845 848 + Csp6I GTAC 845 847 . . 845 848 + RsaI GTAC 846 846 . . 842 846 - BsrI ACTGG 842 840 . . 856 861 + MmeI TCCRAC 881 879 . . 856 860 + Hpy188I TCNGA 858 857 . . 853 857 - MboII GAAGA 845 844 . . 845 857 - BarI GAAGNNNNNNTAC 869 864 837 832 859 869 + Hin4I GAYNNNNNVTC 850 845 882 877 865 868 + SetI ASST 868 864 . . 866 873 + PspXI VCTCGAGB 867 871 . . 866 869 + MnlI CCTC 876 875 . . 867 872 + SmlI CTYRAG 867 871 . . 867 872 + XhoI CTCGAG 867 871 . . 867 872 + BmeT110I CYCGRG 868 870 . . 867 872 + AvaI CYCGRG 867 871 . . 868 871 + TaqI TCGA 868 870 . . 870 875 + SduI GDGCHC 874 870 . . 870 875 + HgiAI* GWGCWC 874 870 . . 873 879 + TspRI CASTGNN 879 870 . . 877 881 + BlsI GCNGC 879 878 . . 877 881 + BisI GCNGC 878 879 . . 877 881 + TseI GCWGC 877 880 . . 873 878 - BtsI GCAGTG 872 870 . . 875 880 - EcoP15I CAGCAG 847 849 . . 880 884 + BlsI GCNGC 882 881 . . 880 884 + BisI GCNGC 881 882 . . 880 884 + TseI GCWGC 880 883 . . 877 881 - BbvI GCAGC 864 868 . . 878 883 - EcoP15I CAGCAG 850 852 . . 880 884 - BbvI GCAGC 867 871 . . 886 890 - MboII GAAGA 878 877 . . 892 896 + Hpy188I TCNGA 894 893 . . 889 893 - MboII GAAGA 881 880 . . 895 905 + Hin4I GAYNNNNNVTC 886 881 918 913 895 898 + DpnI GATC 896 896 . . 895 898 + BstKTI GATC 897 895 . . 895 898 + MboI GATC 894 898 . . 895 899 - BinI* GGATC 889 890 . . 902 905 + MnlI CCTC 912 911 . . 903 908 + SmlI CTYRAG 903 907 . . 903 908 - Bce83I* CTTGAG 888 886 . . 911 916 + NspBII* CMGCKG 913 913 . . 911 914 + AciI CCGC 911 913 . . 913 917 + BlsI GCNGC 915 914 . . 913 917 + BisI GCNGC 914 915 . . 913 917 + TseI GCWGC 913 916 . . 916 920 + TauI GCSGC 919 916 . . 916 920 + BlsI GCNGC 918 917 . . 916 920 + BisI GCNGC 917 918 . . 913 917 - BbvI GCAGC 900 904 . . 917 920 + AciI CCGC 917 919 . . 923 926 + MnlI CCTC 933 932 . . 929 932 + Hin6I GCGC 929 931 . . 929 932 + GlaI GCGC 930 930 . . 924 929 - Eco57MI CTGRAG 909 907 . . 924 929 - GsuI CTGGAG 909 907 . . 929 932 + HhaI GCGC 931 929 . . 930 933 + FnuDII* CGCG 931 931 . . 935 938 + Csp6I GTAC 935 937 . . 935 938 + RsaI GTAC 936 936 . . 946 950 + TauI GCSGC 949 946 . . 946 950 + BlsI GCNGC 948 947 . . 946 950 + BisI GCNGC 947 948 . . 947 950 + AciI CCGC 947 949 . . 952 956 - MboII GAAGA 944 943 . . 956 959 + MaeI CTAG 956 958 . . 959 963 + BlsI GCNGC 961 960 . . 959 963 + BisI GCNGC 960 961 . . 959 963 + BbvI GCAGC 971 975 . . 959 963 + TseI GCWGC 959 962 . . 961 966 + HaeII RGCGCY 965 961 . . 962 965 + Hin6I GCGC 962 964 . . 962 965 + GlaI GCGC 963 963 . . 962 965 + HhaI GCGC 964 962 . . 965 969 + BccI CCATC 973 974 . . 968 973 + Hpy178III* TCNNGA 969 971 . . 969 974 + SmlI CTYRAG 969 973 . . 969 974 + XhoI CTCGAG 969 973 . . 969 974 + BmeT110I CYCGRG 970 972 . . 969 974 + AvaI CYCGRG 969 973 . . 970 973 + TaqI TCGA 970 972 . . 972 977 + SacI GAGCTC 976 972 . . 972 977 + Ecl136II GAGCTC 974 974 . . 972 977 + SduI GDGCHC 976 972 . . 972 977 + HgiJII* GRGCYC 976 972 . . 972 977 + HgiAI* GWGCWC 976 972 . . 973 976 + SetI ASST 976 972 . . 973 976 + CviJI RGCY 974 974 . . 973 976 + AluI AGCT 974 974 . . 974 980 + SapI GCTCTTC 981 984 . . 975 980 + Ksp632I* CTCTTC 981 984 . . 976 980 - MboII GAAGA 968 967 . . 978 982 - TspDTI ATGAA 968 966 . . 1002 1012 + BplI GAGNNNNNCTC 993 988 1025 1020 1006 1011 + TatI WGTACW 1006 1010 . . 1006 1011 + ScaI AGTACT 1008 1008 . . 1007 1010 + Csp6I GTAC 1007 1009 . . 1007 1010 + RsaI GTAC 1008 1008 . . 1002 1012 - BplI GAGNNNNNCTC 1025 1020 993 988 1025 1028 + AciI CCGC 1025 1027 . . 1027 1032 + Cac8I GCNNGC 1029 1029 . . 1030 1033 + SetI ASST 1033 1029 . . 1030 1033 + CviJI RGCY 1031 1031 . . 1030 1033 + AluI AGCT 1031 1031 . . 1033 1038 + Hpy178III* TCNNGA 1034 1036 . . 1032 1037 - Eco57MI CTGRAG 1017 1015 . . 1032 1037 - GsuI CTGGAG 1017 1015 . . 1038 1048 + FalI AAGNNNNNCTT 1029 1024 1061 1056 1042 1046 + MaeIII GTNAC 1041 1046 . . 1038 1048 - FalI AAGNNNNNCTT 1061 1056 1029 1024 1051 1054 + TspEI AATT 1050 1054 . . 1058 1061 + MseI TTAA 1058 1060 . . 1060 1063 + TspEI AATT 1059 1063 . . 1067 1070 + MseI TTAA 1067 1069 . . 1074 1077 + CviRI* TGCA 1075 1075 . . 1080 1089 + MslI CAYNNNNRTG 1084 1084 . . 1080 1089 + OliI CACNNNNGTG 1084 1084 . . 1084 1088 + Tsp4CI* ACNGT 1086 1085 . . 1105 1108 + SetI ASST 1108 1104 . . 1106 1109 + MnlI CCTC 1116 1115 . . 1107 1112 + Ksp632I* CTCTTC 1113 1116 . . 1108 1112 - MboII GAAGA 1100 1099 . . 1117 1122 + HaeII RGCGCY 1121 1117 . . 1118 1121 + Hin6I GCGC 1118 1120 . . 1118 1121 + GlaI GCGC 1119 1119 . . 1118 1121 + HhaI GCGC 1120 1118 . . 1121 1124 + MnlI CCTC 1131 1130 . . 1136 1141 + SfeI* CTRYAG 1136 1140 . . 1140 1143 + SetI ASST 1143 1139 . . 1140 1143 + CviJI RGCY 1141 1141 . . 1140 1143 + AluI AGCT 1141 1141 . . 1141 1145 + BlsI GCNGC 1143 1142 . . 1141 1145 + BisI GCNGC 1142 1143 . . 1141 1145 + TseI GCWGC 1141 1144 . . 1141 1145 - BbvI GCAGC 1128 1132 . . 1146 1157 + BdaI TGANNNNNNTCA 1135 1133 1169 1167 1142 1147 - EcoP15I CAGCAG 1114 1116 . . 1146 1157 - BdaI TGANNNNNNTCA 1169 1167 1135 1133 1169 1174 + StyI CCWWGG 1169 1173 . . 1169 1174 + SecI* CCNNGG 1169 1173 . . 1176 1179 + CviJI RGCY 1177 1177 . . 1176 1179 + HaeIII GGCC 1177 1177 . . 1174 1177 - MnlI CCTC 1167 1166 . . 1183 1186 + SetI ASST 1186 1182 . . 1183 1186 + CviJI RGCY 1184 1184 . . 1183 1186 + AluI AGCT 1184 1184 . . 1185 1189 + DdeI CTNAG 1185 1188 . . 1185 1189 + BspCNI CTCAG 1198 1196 . . 1185 1189 + BseMII CTCAG 1199 1197 . . 1192 1196 + Hpy188I TCNGA 1194 1193 . . 1201 1206 + SpeI ACTAGT 1201 1205 . . 1202 1205 + MaeI CTAG 1202 1204 . . 1210 1214 + MaeIII GTNAC 1209 1214 . . 1213 1218 + SpeI ACTAGT 1213 1217 . . 1214 1217 + MaeI CTAG 1214 1216 . . 1218 1221 + CviRI* TGCA 1219 1219 . . 1219 1229 + MwoI GCNNNNNNNGC 1225 1222 . . 1219 1229 + BstAPI GCANNNNNTGC 1225 1222 . . 1228 1232 + BlsI GCNGC 1230 1229 . . 1228 1232 + BisI GCNGC 1229 1230 . . 1228 1232 + TseI GCWGC 1228 1231 . . 1231 1241 + MwoI GCNNNNNNNGC 1237 1234 . . 1228 1232 - BbvI GCAGC 1215 1219 . . 1239 1242 + CviRI* TGCA 1240 1240 . . 1247 1256 + PshAI GACNNNNGTC 1251 1251 . . 1245 1249 - BsmAI GTCTC 1239 1243 . . 1261 1266 + Hpy178III* TCNNGA 1262 1264 . . 1258 1262 - MboII GAAGA 1250 1249 . . 1265 1274 + XmnI GAANNNNTTC 1269 1269 . . 1270 1274 - MboII GAAGA 1262 1261 . . 1274 1278 + BssKI CCNGG 1273 1278 . . 1274 1277 + HpaII CCGG 1274 1276 . . 1274 1279 + SecI* CCNNGG 1274 1278 . . 1274 1278 + ScrFI CCNGG 1275 1276 . . 1274 1278 + CauII* CCSGG 1275 1276 . . 1276 1281 + NlaIV GGNNCC 1278 1278 . . 1277 1281 + BmgT120I GGNCC 1278 1279 . . 1277 1281 + AsuI* GGNCC 1277 1280 . . 1278 1281 + CviJI RGCY 1279 1279 . . 1278 1281 + HaeIII GGCC 1279 1279 . . 1281 1286 + Esp3I CGTCTC 1287 1291 . . 1282 1286 + BsmAI GTCTC 1287 1291 . . 1279 1283 - BceAI ACGGC 1264 1266 . . 1284 1289 + Ksp632I* CTCTTC 1290 1293 . . 1289 1292 + MnlI CCTC 1299 1298 . . 1285 1289 - MboII GAAGA 1277 1276 . . 1289 1295 + Bpu10I CCTNAGC 1290 1293 . . 1289 1295 + BbvCI CCTCAGC 1290 1293 . . 1290 1294 + DdeI CTNAG 1290 1293 . . 1290 1294 + BspCNI CTCAG 1303 1301 . . 1290 1294 + BseMII CTCAG 1304 1302 . . 1292 1297 + PvuII CAGCTG 1294 1294 . . 1292 1297 + NspBII* CMGCKG 1294 1294 . . 1293 1296 + SetI ASST 1296 1292 . . 1293 1296 + CviJI RGCY 1294 1294 . . 1293 1296 + AluI AGCT 1294 1294 . . 1311 1315 + Hpy188I TCNGA 1313 1312 . . 1314 1319 + EcoRI GAATTC 1314 1318 . . 1314 1319 + ApoI RAATTY 1314 1318 . . 1315 1318 + TspEI AATT 1314 1318 . . 1318 1322 - TspGWI ACGGA 1308 1306 . . 1326 1329 + CviJI RGCY 1327 1327 . . 1330 1335 + Hpy166II GTNNAC 1332 1332 . . 1330 1335 + HindII GTYRAC 1332 1332 . . 1327 1331 - BceAI ACGGC 1312 1314 . . 1336 1346 + BaeI ACNNNNGTAYC 1325 1320 1358 1353 1338 1342 + Hpy99I CGWCG 1342 1337 . . 1340 1343 + TaiI ACGT 1343 1339 . . 1340 1343 + SetI ASST 1343 1339 . . 1340 1343 + MaeII ACGT 1340 1342 . . 1348 1352 + MaeIII GTNAC 1347 1352 . . 1351 1354 + SetI ASST 1354 1350 . . 1352 1355 + MnlI CCTC 1362 1361 . . 1353 1357 + DdeI CTNAG 1353 1356 . . 1353 1357 + BspCNI CTCAG 1366 1364 . . 1353 1357 + BseMII CTCAG 1367 1365 . . 1368 1371 + CviJI RGCY 1369 1369 . . 1388 1391 + AciI CCGC 1388 1390 . . 1402 1406 + MaeIII GTNAC 1401 1406 . . 1397 1402 - Eco57MI CTGRAG 1382 1380 . . 1397 1402 - Eco57I CTGAAG 1382 1380 . . 1406 1409 + AciI CCGC 1406 1408 . . 1409 1412 + MnlI CCTC 1419 1418 . . 1410 1414 + DdeI CTNAG 1410 1413 . . 1410 1414 + BspCNI CTCAG 1423 1421 . . 1410 1414 + BseMII CTCAG 1424 1422 . . 1411 1415 + Hpy188I TCNGA 1413 1412 . . 1416 1426 + FalI AAGNNNNNCTT 1407 1402 1439 1434 1426 1430 + Hpy188I TCNGA 1428 1427 . . 1416 1426 - FalI AAGNNNNNCTT 1439 1434 1407 1402 1436 1440 + DdeI CTNAG 1436 1439 . . 1436 1440 + BspCNI CTCAG 1449 1447 . . 1436 1440 + BseMII CTCAG 1450 1448 . . 1440 1443 + CviJI RGCY 1441 1441 . . 1435 1441 - Bpu10I CCTNAGC 1436 1439 . . 1458 1462 + MboII GAAGA 1470 1469 . . 1462 1467 + SspI AATATT 1464 1464 . . 1469 1473 + MaeIII GTNAC 1468 1473 . . 1472 1476 + Tsp4CI* ACNGT 1474 1473 . . 1473 1479 + TspRI CASTGNN 1479 1470 . . 1477 1481 + TauI GCSGC 1480 1477 . . 1477 1481 + BlsI GCNGC 1479 1478 . . 1477 1481 + BisI GCNGC 1478 1479 . . 1478 1481 + AciI CCGC 1478 1480 . . 1486 1489 + TaqI TCGA 1486 1488 . . 1501 1506 + MmeI TCCRAC 1526 1524 . . 1505 1509 + Tsp4CI* ACNGT 1507 1506 . . 1506 1512 + TspRI CASTGNN 1512 1503 . . 1519 1524 + Hpy178III* TCNNGA 1520 1522 . . 1526 1530 + Tsp4CI* ACNGT 1528 1527 . . 1531 1535 + MaeIII GTNAC 1530 1535 . . 1540 1544 + BlsI GCNGC 1542 1541 . . 1540 1544 + BisI GCNGC 1541 1542 . . 1540 1544 + TseI GCWGC 1540 1543 . . 1543 1553 + MwoI GCNNNNNNNGC 1549 1546 . . 1540 1544 - BbvI GCAGC 1527 1531 . . 1561 1565 + Hpy188I TCNGA 1563 1562 . . 1559 1563 - FokI GGATG 1545 1549 . . 1559 1563 - BseGI GGATG 1558 1556 . . 1564 1574 + BplI GAGNNNNNCTC 1555 1550 1587 1582 1566 1577 + AlfI GCANNNNNNTGC 1555 1553 1589 1587 1571 1574 + MnlI CCTC 1581 1580 . . 1564 1574 - BplI GAGNNNNNCTC 1587 1582 1555 1550 1576 1579 + Hin6I GCGC 1576 1578 . . 1576 1579 + GlaI GCGC 1577 1577 . . 1576 1579 + HhaI GCGC 1578 1576 . . 1566 1577 - AlfI GCANNNNNNTGC 1589 1587 1555 1553 1578 1583 + Cac8I GCNNGC 1580 1580 . . 1584 1593 + MslI CAYNNNNRTG 1588 1588 . . 1584 1593 + OliI CACNNNNGTG 1588 1588 . . 1593 1598 + Cac8I GCNNGC 1595 1595 . . 1603 1606 + TspEI AATT 1602 1606 . . 1610 1613 + MaeI CTAG 1610 1612 . . 1616 1621 + Hpy178III* TCNNGA 1617 1619 . . 1620 1624 + MboII GAAGA 1632 1631 . . 1620 1625 + BbvII* GAAGAC 1627 1631 . . 1638 1643 + ApoI RAATTY 1638 1642 . . 1639 1642 + TspEI AATT 1638 1642 . . 1642 1645 + TaqI TCGA 1642 1644 . . 1646 1649 + Csp6I GTAC 1646 1648 . . 1646 1649 + RsaI GTAC 1647 1647 . . 1655 1659 + BccI CCATC 1663 1664 . . 1662 1665 + CviJI RGCY 1663 1663 . . 1670 1674 + DdeI CTNAG 1670 1673 . . 1675 1680 + TatI WGTACW 1675 1679 . . 1675 1680 + ScaI AGTACT 1677 1677 . . 1676 1679 + Csp6I GTAC 1676 1678 . . 1676 1679 + RsaI GTAC 1677 1677 . . 1684 1687 + SetI ASST 1687 1683 . . 1685 1690 + StyI CCWWGG 1685 1689 . . 1685 1690 + SecI* CCNNGG 1685 1689 . . 1688 1693 + BalI TGGCCA 1690 1690 . . 1688 1693 + CfrI YGGCCR 1688 1692 . . 1689 1692 + CviJI RGCY 1690 1690 . . 1689 1692 + HaeIII GGCC 1690 1690 . . 1690 1700 + MwoI GCNNNNNNNGC 1696 1693 . . 1717 1727 + MwoI GCNNNNNNNGC 1723 1720 . . 1717 1721 + BlsI GCNGC 1719 1718 . . 1717 1721 + BisI GCNGC 1718 1719 . . 1717 1721 + BbvI GCAGC 1729 1733 . . 1717 1721 + TseI GCWGC 1717 1720 . . 1719 1722 + SetI ASST 1722 1718 . . 1719 1722 + CviJI RGCY 1720 1720 . . 1719 1722 + AluI AGCT 1720 1720 . . 1724 1727 + AciI CCGC 1724 1726 . . 1729 1733 + MaeIII GTNAC 1728 1733 . . 1730 1735 + TsoI TARCCA 1746 1744 . . 1737 1742 + XhoII RGATCY 1737 1741 . . 1738 1741 + DpnI GATC 1739 1739 . . 1738 1741 + BstKTI GATC 1740 1738 . . 1738 1741 + MboI GATC 1737 1741 . . 1738 1742 - BinI* GGATC 1732 1733 . . 1749 1754 + NlaIV GGNNCC 1751 1751 . . 1749 1753 + BmgT120I GGNCC 1750 1751 . . 1749 1753 + AvaII GGWCC 1749 1752 . . 1749 1753 + AsuI* GGNCC 1749 1752 . . 1764 1768 + DdeI CTNAG 1764 1767 . . 1764 1768 + BspCNI CTCAG 1777 1775 . . 1764 1768 + BseMII CTCAG 1778 1776 . . 1767 1770 + SetI ASST 1770 1766 . . 1767 1770 + CviJI RGCY 1768 1768 . . 1767 1770 + AluI AGCT 1768 1768 . . 1769 1772 + MaeI CTAG 1769 1771 . . 1773 1778 + MmeI TCCRAC 1798 1796 . . 1795 1798 + SetI ASST 1798 1794 . . 1796 1799 + MnlI CCTC 1806 1805 . . 1802 1807 + NdeI CATATG 1803 1805 . . 1807 1812 + XhoII RGATCY 1807 1811 . . 1807 1811 + BinI* GGATC 1815 1816 . . 1808 1811 + DpnI GATC 1809 1809 . . 1808 1811 + BstKTI GATC 1810 1808 . . 1808 1811 + MboI GATC 1807 1811 . . 1810 1814 - MboII GAAGA 1802 1801 . . 1813 1817 - MboII GAAGA 1805 1804 . . 1823 1826 + Hin6I GCGC 1823 1825 . . 1823 1826 + GlaI GCGC 1824 1824 . . 1823 1826 + HhaI GCGC 1825 1823 . . 1825 1829 + SfaNI GCATC 1834 1838 . . 1837 1840 + SetI ASST 1840 1836 . . 1837 1840 + CviJI RGCY 1838 1838 . . 1837 1840 + AluI AGCT 1838 1838 . . 1853 1857 - BsrI ACTGG 1853 1851 . . 1864 1868 + BsmAI GTCTC 1869 1873 . . 1866 1871 + SmlI CTYRAG 1866 1870 . . 1866 1871 - Bce83I* CTTGAG 1851 1849 . . 1881 1885 + Tsp4CI* ACNGT 1883 1882 . . 1888 1892 + BsmAI GTCTC 1893 1897 . . 1894 1897 + SetI ASST 1897 1893 . . 1894 1899 + BspMI ACCTGC 1903 1907 . . 1897 1900 + CviRI* TGCA 1898 1898 . . 1896 1901 - BsgI GTGCAG 1881 1879 . . 1903 1907 + Hpy188I TCNGA 1905 1904 . . 1918 1922 + Hpy188I TCNGA 1920 1919 . . 1928 1931 + MaeI CTAG 1928 1930 . . 1930 1933 + SetI ASST 1933 1929 . . 1930 1933 + CviJI RGCY 1931 1931 . . 1930 1933 + AluI AGCT 1931 1931 . . 1937 1941 + BccI CCATC 1945 1946 . . 1944 1950 + TspRI CASTGNN 1950 1941 . . 1944 1949 - BtsI GCAGTG 1943 1941 . . 1956 1962 + TspRI CASTGNN 1962 1953 . . 1968 1972 + Hpy188I TCNGA 1970 1969 . . 1970 1975 + BclI TGATCA 1970 1974 . . 1971 1974 + DpnI GATC 1972 1972 . . 1971 1974 + BstKTI GATC 1973 1971 . . 1971 1974 + MboI GATC 1970 1974 . . 1982 1986 + BccI CCATC 1990 1991 . . 1989 1992 + SetI ASST 1992 1988 . . 1989 1992 + CviJI RGCY 1990 1990 . . 1989 1992 + AluI AGCT 1990 1990 . . 2000 2003 + Csp6I GTAC 2000 2002 . . 2000 2003 + RsaI GTAC 2001 2001 . . 2002 2006 + TspGWI ACGGA 2017 2015 . . 2011 2014 + TspEI AATT 2010 2014 . . 2020 2023 + SetI ASST 2023 2019 . . 2021 2024 + MnlI CCTC 2031 2030 . . 2024 2029 + NspBII* CMGCKG 2026 2026 . . 2024 2027 + AciI CCGC 2024 2026 . . 2026 2030 + BlsI GCNGC 2028 2027 . . 2026 2030 + BisI GCNGC 2027 2028 . . 2026 2030 + TseI GCWGC 2026 2029 . . 2026 2030 - BbvI GCAGC 2013 2017 . . 2032 2036 - MboII GAAGA 2024 2023 . . 2036 2041 + SfeI* CTRYAG 2036 2040 . . 2047 2051 + BceAI ACGGC 2063 2065 . . 2044 2048 - BsmAI GTCTC 2038 2042 . . 2049 2052 + CviJI RGCY 2050 2050 . . 2044 2049 - Esp3I CGTCTC 2038 2042 . . 2049 2052 + HaeIII GGCC 2050 2050 . . 2051 2055 + Hin4II* CCTTC 2061 2060 . . 2057 2060 + MaeI CTAG 2057 2059 . . 2066 2071 + Hpy178III* TCNNGA 2067 2069 . . 2068 2071 + TaqI TCGA 2068 2070 . . 2088 2099 + PpiI GAACNNNNNCTC 2080 2075 2112 2107 2090 2094 + BceAI ACGGC 2106 2108 . . 2092 2097 + NarI GGCGCC 2093 2095 . . 2092 2097 + KasI GGCGCC 2092 2096 . . 2092 2097 + DinI GGCGCC 2094 2094 . . 2092 2097 + HaeII RGCGCY 2096 2092 . . 2092 2097 + NlaIV GGNNCC 2094 2094 . . 2092 2097 + AcyI GRCGYC 2093 2095 . . 2092 2097 + HgiCI* GGYRCC 2092 2096 . . 2093 2096 + Hin6I GCGC 2093 2095 . . 2093 2096 + GlaI GCGC 2094 2094 . . 2093 2096 + HhaI GCGC 2095 2093 . . 2096 2099 + MnlI CCTC 2106 2105 . . 2109 2115 + TspRI CASTGNN 2115 2106 . . 2108 2112 - BsrI ACTGG 2108 2106 . . 2114 2117 + SetI ASST 2117 2113 . . 2114 2117 + CviJI RGCY 2115 2115 . . 2114 2117 + AluI AGCT 2115 2115 . . 2117 2122 + TsoI TARCCA 2133 2131 . . 2123 2128 - BtgZI GCGATG 2108 2112 . . 2131 2135 + TspDTI ATGAA 2146 2144 . . 2138 2149 + BstXI CCANNNNNNTGG 2145 2141 . . 2142 2153 + TstI CACNNNNNNTCC 2133 2128 2165 2160 2143 2147 + Tsp4CI* ACNGT 2145 2144 . . 2148 2153 + NlaIV GGNNCC 2150 2150 . . 2154 2157 + SetI ASST 2157 2153 . . 2154 2157 + CviJI RGCY 2155 2155 . . 2154 2157 + AluI AGCT 2155 2155 . . 2158 2162 - MboII GAAGA 2150 2149 . . 2167 2171 + Hin4II* CCTTC 2177 2176 . . 2187 2192 + XbaI TCTAGA 2187 2191 . . 2187 2192 + Hpy178III* TCNNGA 2188 2190 . . 2188 2191 + MaeI CTAG 2188 2190 . . 2202 2208 + TspRI CASTGNN 2208 2199 . . 2202 2207 - BtsI GCAGTG 2201 2199 . . 2213 2216 + MaeI CTAG 2213 2215 . . 2223 2229 + TspRI CASTGNN 2229 2220 . . 2225 2230 + Eco57MI CTGRAG 2246 2244 . . 2225 2230 + Eco57I CTGAAG 2246 2244 . . 2230 2235 + Hpy166II GTNNAC 2232 2232 . . 2230 2235 + HindII GTYRAC 2232 2232 . . 2236 2240 + TauI GCSGC 2239 2236 . . 2236 2240 + BlsI GCNGC 2238 2237 . . 2236 2240 + BisI GCNGC 2237 2238 . . 2237 2240 + AciI CCGC 2237 2239 . . 2249 2252 + MseI TTAA 2249 2251 . . 2253 2258 + SecI* CCNNGG 2253 2257 . . 2254 2258 + BssKI CCNGG 2253 2258 . . 2254 2258 + ScrFI CCNGG 2255 2256 . . 2254 2258 + CauII* CCSGG 2255 2256 . . 2255 2258 + HpaII CCGG 2255 2257 . . 2266 2270 - MboII GAAGA 2258 2257 . . 2275 2280 + Cac8I GCNNGC 2277 2277 . . 2275 2280 + BmtI GCTAGC 2279 2275 . . 2275 2280 + NheI GCTAGC 2275 2279 . . 2276 2279 + MaeI CTAG 2276 2278 . . 2293 2297 + Hpy188I TCNGA 2295 2294 . . 2300 2303 + CviJI RGCY 2301 2301 . . 2301 2311 + MwoI GCNNNNNNNGC 2307 2304 . . 2308 2313 + SduI GDGCHC 2312 2308 . . 2308 2313 + HgiJII* GRGCYC 2312 2308 . . 2309 2312 + CviJI RGCY 2310 2310 . . 2329 2332 + SetI ASST 2332 2328 . . 2330 2333 + MnlI CCTC 2340 2339 . . 2341 2346 + NlaIV GGNNCC 2343 2343 . . 2341 2346 + HgiCI* GGYRCC 2341 2345 . . 2338 2342 - Hin4II* CCTTC 2332 2331 . . 2344 2347 + SetI ASST 2347 2343 . . 2345 2348 + MnlI CCTC 2355 2354 . . 2346 2351 + Ksp632I* CTCTTC 2352 2355 . . 2347 2351 - MboII GAAGA 2339 2338 . . 2362 2365 + SetI ASST 2365 2361 . . 2374 2378 + Tsp4CI* ACNGT 2376 2375 . . 2384 2390 + TspRI CASTGNN 2390 2381 . . 2385 2389 + Tsp4CI* ACNGT 2387 2386 . . 2396 2399 + CviJI RGCY 2397 2397 . . 2399 2402 + MnlI CCTC 2409 2408 . . 2400 2405 - Eco57MI CTGRAG 2385 2383 . . 2400 2405 - GsuI CTGGAG 2385 2383 . . 2410 2413 + CviJI RGCY 2411 2411 . . 2412 2417 + StyI CCWWGG 2412 2416 . . 2412 2417 + SecI* CCNNGG 2412 2416 . . 2412 2417 + AvrII CCTAGG 2412 2416 . . 2413 2416 + MaeI CTAG 2413 2415 . . 2415 2418 + SetI ASST 2418 2414 . . 2418 2421 + CviRI* TGCA 2419 2419 . . 2431 2435 + BsrI ACTGG 2436 2434 . . 2441 2445 + Tsp4CI* ACNGT 2443 2442 . . 2442 2447 + McrI* CGRYCG 2445 2443 . . 2458 2461 + CviJI RGCY 2459 2459 . . 2478 2481 + FatI CATG 2477 2481 . . 2478 2481 + NlaIII CATG 2481 2477 . . 2478 2481 + CviAII CATG 2478 2480 . . 2479 2484 + EcoT22I ATGCAT 2483 2479 . . 2480 2483 + CviRI* TGCA 2481 2481 . . 2481 2485 + SfaNI GCATC 2490 2494 . . 2486 2489 + CviJI RGCY 2487 2487 . . 2495 2498 + MnlI CCTC 2505 2504 . . 2497 2500 + TaqI TCGA 2497 2499 . . 2499 2509 + Hin4I GAYNNNNNVTC 2490 2485 2522 2517 2499 2502 + DpnI GATC 2500 2500 . . 2499 2502 + BstKTI GATC 2501 2499 . . 2499 2502 + MboI GATC 2498 2502 . . 2518 2522 - Hin4II* CCTTC 2512 2511 . . 2534 2537 + AciI CCGC 2534 2536 . . 2547 2550 + CviRI* TGCA 2548 2548 . . 2553 2564 + BcgI CGANNNNNNTGC 2542 2540 2576 2574 2556 2559 + SetI ASST 2559 2555 . . 2554 2558 - Hin4II* CCTTC 2548 2547 . . 2560 2564 + BlsI GCNGC 2562 2561 . . 2560 2564 + BisI GCNGC 2561 2562 . . 2560 2564 + TseI GCWGC 2560 2563 . . 2562 2565 + CviRI* TGCA 2563 2563 . . 2560 2564 - BbvI GCAGC 2547 2551 . . 2568 2571 + SetI ASST 2571 2567 . . 2569 2572 + MnlI CCTC 2579 2578 . . 2584 2590 + TspRI CASTGNN 2590 2581 . . 2587 2590 + CviRI* TGCA 2588 2588 . . 2583 2587 - BsrI ACTGG 2583 2581 . . 2596 2599 + MaeI CTAG 2596 2598 . . 2598 2601 + SetI ASST 2601 2597 . . 2598 2601 + CviJI RGCY 2599 2599 . . 2598 2601 + AluI AGCT 2599 2599 . . 2611 2615 + TauI GCSGC 2614 2611 . . 2611 2615 + BlsI GCNGC 2613 2612 . . 2611 2615 + BisI GCNGC 2612 2613 . . 2612 2615 + AciI CCGC 2612 2614 . . 2625 2628 + SetI ASST 2628 2624 . . 2625 2628 + CviJI RGCY 2626 2626 . . 2625 2628 + AluI AGCT 2626 2626 . . 2643 2646 + MaeI CTAG 2643 2645 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 589 2057 # # AciI: # [CCGC] # 6 18 30 34 38 49 # 72 78 78 100 132 133 # 207 213 246 252 297 300 # 363 # # AcyI: # [GRCGYC] # 272 553 1821 # # AflIII: # [ACRYGT] # 117 2529 # # AhaIII*: # [TTTAAA] # 629 2017 # # AlfI: # [GCANNNNNNTGC] # 0 0 34 1057 1555 # # AluI: # [AGCT] # 20 27 40 43 48 57 # 59 70 93 110 110 125 # 231 426 444 743 # # ApoI: # [RAATTY] # 324 1008 1314 # # AsuI*: # [GGNCC] # 77 143 472 897 1057 # # AvaI: # [CYCGRG] # 93 102 774 1677 # # AvaII: # [GGWCC] # 143 897 1606 # # AvrII: # [CCTAGG] # 234 2412 # # BaeI: # [ACNNNNGTAYC] # 33 33 584 708 1288 # # BalI: # [TGGCCA] # 157 956 1533 # # BamHI: # [GGATCC] # 715 1931 # # BarI: # [GAAGNNNNNNTAC] # 32 837 1777 # # BbvCI: # [CCTCAGC] # 1290 1356 # # BbvI: # [GCAGC] # 3 33 71 87 99 157 # 202 235 284 312 534 629 # # BbvII*: # [GAAGAC] # 1019 1627 # # BccI: # [CCATC] # 45 211 282 348 414 656 # 690 # # Bce83I*: # [CTTGAG] # 165 723 795 963 # # BceAI: # [ACGGC] # 28 43 48 540 751 1236 # # BcgI: # [CGANNNNNNTGC] # 34 70 2542 # # BclI: # [TGATCA] # 676 1970 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 1135 1477 # # BglI: # [GCCNNNNNGGC] # 49 2597 # # BinI*: # [GGATC] # 13 83 166 710 831 843 # # BisI: # [GCNGC] # 3 3 7 13 30 33 # 34 51 63 87 133 177 # 182 210 249 260 309 324 # 478 # # BlsI: # [GCNGC] # 3 3 7 13 30 33 # 33 51 63 87 134 177 # 182 210 249 260 309 324 # 478 # # BmeT110I: # [CYCGRG] # 93 102 775 1676 # # BmgT120I: # [GGNCC] # 77 144 472 896 1057 # # BmtI: # [GCTAGC] # 367 617 1662 # # BplI: # [GAGNNNNNCTC] # 0 0 0 0 32 32 # 530 993 1059 # # Bpu10I: # [CCTNAGC] # 146 1210 1290 # # BsaXI: # [ACNNNNNCTCC] # 30 529 2087 # # BseBI: # [CCWGG] # 11 135 141 164 2195 # # BseGI: # [GGATG] # 37 51 123 249 345 753 # 1088 # # BseMII: # [CTCAG] # 26 57 63 105 328 868 # 1199 # # BseRI: # [GAGGAG] # 762 1884 # # BsgI: # [GTGCAG] # 765 1881 # # BsiYI*: # [CCNNNNNNNGG] # 281 2365 # # BsmAI: # [GTCTC] # 24 48 60 145 183 447 # 549 582 608 # # BsmI: # [GAATGC] # 210 2436 # # BspCNI: # [CTCAG] # 26 57 63 105 328 869 # 1198 # # BspLU11I*: # [ACATGT] # 117 2529 # # BspMI: # [ACCTGC] # 743 1903 # # BsrBI: # [CCGCTC] # 130 135 2381 # # BsrI: # [ACTGG] # 63 147 255 282 328 560 # 1011 # # BssKI: # [CCNGG] # 11 38 126 135 139 393 # 824 980 # # BssNAI: # [GTATAC] # 590 2056 # # BstAPI: # [GCANNNNNTGC] # 1225 1421 # # BstKTI: # [GATC] # 70 145 163 179 528 718 # 843 # # BstXI: # [CCANNNNNNTGG] # 501 2145 # # BtgZI: # [GCGATG] # 95 538 2013 # # BtsI: # [GCAGTG] # 204 258 445 668 1071 # # Cac8I: # [GCNNGC] # 15 43 369 414 551 572 # 682 # # CauII*: # [CCSGG] # 314 391 961 980 # # CfrI: # [YGGCCR] # 155 958 1533 # # Csp6I: # [GTAC] # 30 66 72 90 119 279 # 324 381 639 646 # # CviAII: # [CATG] # 118 168 286 2074 # # CviJI: # [RGCY] # 7 7 9 14 15 20 # 27 27 28 30 33 36 # 40 42 43 48 48 57 # 59 60 64 65 70 72 # 87 93 95 110 112 114 # 123 146 222 231 392 # # CviRI*: # [TGCA] # 15 21 25 58 62 67 # 144 463 521 612 658 # # DdeI: # [CTNAG] # 26 57 63 94 105 234 # 882 1185 # # DinI: # [GGCGCC] # 552 2094 # # DpnI: # [GATC] # 70 146 163 179 528 717 # 843 # # DsaI*: # [CCRYGG] # 329 2317 # # Ecl136II: # [GAGCTC] # 974 1672 # # Eco47III: # [AGCGCT] # 113 2533 # # Eco57I: # [CTGAAG] # 400 864 1382 # # Eco57MI: # [CTGRAG] # 25 108 139 261 365 864 # 884 # # EcoP15I: # [CAGCAG] # 3 199 264 648 1532 # # EcoRI: # [GAATTC] # 1314 1332 # # EcoRII: # [CCWGG] # 11 135 139 164 2197 # # EcoT22I: # [ATGCAT] # 163 2483 # # Esp3I: # [CGTCTC] # 495 608 751 792 # # FalI: # [AAGNNNNNCTT] # 0 0 0 0 32 32 # 346 1029 1207 # # FatI: # [CATG] # 117 169 286 2074 # # FauI: # [CCCGC] # 579 2067 # # FnuDII*: # [CGCG] # 931 1715 # # FokI: # [GGATG] # 44 51 103 249 345 753 # 1101 # # GlaI: # [GCGC] # 18 33 36 113 156 247 # 270 349 414 458 552 # # GsuI: # [CTGGAG] # 25 108 261 884 1368 # # HaeII: # [RGCGCY] # 18 115 156 550 832 975 # # HaeIII: # [GGCC] # 64 102 157 360 411 596 # 956 # # HgaI: # [GACGC] # 280 382 1984 # # HgiAI*: # [GWGCWC] # 102 427 447 1670 # # HgiCI*: # [GGYRCC] # 249 305 2092 # # HgiJII*: # [GRGCYC] # 334 976 1336 # # HhaI: # [GCGC] # 18 33 36 114 156 247 # 270 349 414 458 551 # # Hin4I: # [GAYNNNNNVTC] # 4 32 32 32 32 124 # 289 529 1572 # # Hin4II*: # [CCTTC] # 36 98 116 155 180 2061 # # Hin6I: # [GCGC] # 18 33 36 112 156 247 # 270 349 414 458 553 # # HindII: # [GTYRAC] # 123 239 414 900 970 # # HinfI: # [GANTC] # 325 381 1940 # # HpaII: # [CCGG] # 314 391 960 981 # # HphI: # [GGTGA] # 112 129 225 2180 # # Hpy166II: # [GTNNAC] # 92 123 147 228 414 742 # 900 # # Hpy178III*: # [TCNNGA] # 65 75 97 121 228 258 # 450 458 894 # # Hpy188I: # [TCNGA] # 15 15 36 38 50 69 # 100 108 109 119 135 213 # 300 321 325 342 351 # # Hpy99I: # [CGWCG] # 148 555 639 1304 # # KasI: # [GGCGCC] # 554 2092 # # Ksp632I*: # [CTCTTC] # 66 132 162 177 231 294 # 522 1062 # # MaeI: # [CTAG] # 3 9 12 25 28 47 # 63 81 96 129 131 137 # 159 159 165 183 246 396 # 577 # # MaeII: # [ACGT] # 61 1279 1306 # # MaeIII: # [GTNAC] # 54 62 67 87 138 168 # 198 247 291 416 918 # # MboI: # [GATC] # 70 148 163 179 528 715 # 843 # # MboII: # [GAAGA] # 3 3 12 15 24 33 # 63 66 81 108 108 126 # 132 150 162 162 170 193 # 219 307 509 # # McrI*: # [CGRYCG] # 201 2445 # # MmeI: # [TCCRAC] # 170 188 272 523 645 848 # # MnlI: # [CCTC] # 3 6 6 6 9 11 # 13 15 15 18 21 33 # 36 36 42 45 48 48 # 54 57 63 67 74 75 # 96 132 142 162 183 225 # 225 234 446 # # MseI: # [TTAA] # 9 205 397 423 430 1182 # # MslI: # [CAYNNNNRTG] # 131 215 504 738 1058 # # MstI*: # [TGCGCA] # 167 2479 # # MwoI: # [GCNNNNNNNGC] # 12 27 44 49 70 108 # 147 312 339 387 567 584 # # NarI: # [GGCGCC] # 553 2093 # # NdeI: # [CATATG] # 225 843 1578 # # NheI: # [GCTAGC] # 371 613 1662 # # NlaIII: # [CATG] # 121 165 286 2074 # # NlaIV: # [GGNNCC] # 56 193 241 303 343 473 # 476 561 # # NmeAIII: # [GCCGAG] # 393 2253 # # NspBII*: # [CMGCKG] # 339 381 574 620 732 # # NspI: # [RCATGY] # 121 2525 # # OliI: # [CACNNNNGTG] # 346 504 738 1058 # # PfoI: # [TCCNGGA] # 126 312 2208 # # PpiI: # [GAACNNNNNCTC] # 32 534 2080 # # PshAI: # [GACNNNNGTC] # 1251 1395 # # PsiI: # [TTATAA] # 98 386 2162 # # PspXI: # [VCTCGAGB] # 93 774 1779 # # PvuII: # [CAGCTG] # 1294 1352 # # RsaI: # [GTAC] # 30 66 72 90 119 280 # 324 381 639 645 # # SacI: # [GAGCTC] # 976 1670 # # SapI: # [GCTCTTC] # 231 750 1665 # # ScaI: # [AGTACT] # 669 969 1008 # # ScrFI: # [CCNGG] # 11 38 126 135 141 391 # 824 980 # # SduI: # [GDGCHC] # 102 334 427 447 1336 # # SecI*: # [CCNNGG] # 86 105 121 159 234 243 # 411 568 719 # # SetI: # [ASST] # 11 12 15 18 18 21 # 27 27 28 30 31 35 # 35 36 40 42 43 45 # 47 48 51 53 57 57 # 59 64 74 75 76 78 # 94 102 108 110 111 141 # 175 219 333 # # SexAI: # [ACCWGGT] # 139 2507 # # SfaNI: # [GCATC] # 22 156 656 764 1048 # # SfeI*: # [CTRYAG] # 561 575 610 900 # # SmlI: # [CTYRAG] # 36 36 66 93 738 780 # 897 # # SpeI: # [ACTAGT] # 12 507 694 1433 # # SspI: # [AATATT] # 391 1073 1182 # # StyI: # [CCWWGG] # 234 243 516 727 926 # # TaiI: # [ACGT] # 64 1279 1303 # # TaqI: # [TCGA] # 74 93 102 141 149 156 # 426 429 516 560 # # TaqII: # [CACCCA] # 442 2204 # # TatI: # [WGTACW] # 669 971 1006 # # TauI: # [GCSGC] # 30 32 135 306 375 478 # 531 759 # # TfiI: # [GAWTC] # 325 381 1940 # # TseI: # [GCWGC] # 3 33 46 86 87 177 # 182 260 309 312 534 617 # # TsoI: # [TARCCA] # 135 236 387 513 1375 # # Tsp45I: # [GTSAC] # 334 416 1896 # # Tsp4CI*: # [ACNGT] # 11 21 33 39 51 56 # 57 57 87 203 231 262 # 303 355 388 492 # # TspDTI: # [ATGAA] # 174 500 794 1178 # # TspEI: # [AATT] # 9 36 111 255 278 288 # 300 361 372 636 # # TspGWI: # [ACGGA] # 629 709 1308 # # TspRI: # [CASTGNN] # 12 21 33 54 56 93 # 150 153 161 180 200 438 # 495 600 # # TstI: # [CACNNNNNNTCC] # 32 481 2133 # # Tth111I: # [GACNNNGTC] # 541 2105 # # XbaI: # [TCTAGA] # 459 2187 # # XhoI: # [CTCGAG] # 93 102 774 1677 # # XhoII: # [RGATCY] # 70 715 839 1022 # # XmnI: # [GAANNNNTTC] # 1269 1377 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 2646 # Reported_sequences: 1 # Reported_hitcount: 783 #---------------------------------------