Restriction Fragments for JEM1/YJL073W



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The currently selected gene/sequence is :

JEM1/YJL073W on chromosome X from coordinates 303181 to 305118.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.15626.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 3 12 + BsaBI GATNNNNATC 7 7 . . 9 12 + DpnI GATC 10 10 . . 9 12 + BstKTI GATC 11 9 . . 9 12 + MboI GATC 8 12 . . 12 17 + BmeT110I CYCGRG 13 15 . . 12 17 + AvaI CYCGRG 12 16 . . 13 18 + Hpy178III* TCNNGA 14 16 . . 20 24 + Tsp4CI* ACNGT 22 21 . . 16 21 - BciVI GTATCC 10 9 . . 51 56 + TatI WGTACW 51 55 . . 51 56 + ScaI AGTACT 53 53 . . 52 55 + Csp6I GTAC 52 54 . . 52 55 + RsaI GTAC 53 53 . . 49 53 - BsrI ACTGG 49 47 . . 57 62 + EcoRV GATATC 59 59 . . 63 66 + CviJI RGCY 64 64 . . 68 72 + DdeI CTNAG 68 71 . . 84 87 + CviJI RGCY 85 85 . . 86 90 + DdeI CTNAG 86 89 . . 90 94 + MaeIII GTNAC 89 94 . . 86 90 - BspCNI CTCAG 78 76 . . 86 90 - BseMII CTCAG 77 75 . . 108 113 + ApoI RAATTY 108 112 . . 109 112 + TspEI AATT 108 112 . . 111 116 + AhaIII* TTTAAA 113 113 . . 112 115 + MseI TTAA 112 114 . . 118 123 + Hpy166II GTNNAC 120 120 . . 118 123 + AccI GTMKAC 119 121 . . 121 131 + Hin4I GAYNNNNNVTC 112 107 144 139 123 129 + TspRI CASTGNN 129 120 . . 127 131 + TfiI GAWTC 127 130 . . 127 131 + HinfI GANTC 127 130 . . 121 131 - Hin4I GAYNNNNNVTC 144 139 112 107 136 150 + XcmI CCANNNNNNNNNTGG 143 142 . . 144 158 + XcmI CCANNNNNNNNNTGG 151 150 . . 149 153 + BinI* GGATC 157 158 . . 150 153 + DpnI GATC 151 151 . . 150 153 + BstKTI GATC 152 150 . . 150 153 + MboI GATC 149 153 . . 154 159 - GsaI CCCAGC 158 154 . . 154 159 - BseYI CCCAGC 154 158 . . 162 167 - MmeI TCCRAC 143 141 . . 175 178 + CviRI* TGCA 176 176 . . 177 180 + FatI CATG 176 180 . . 177 180 + NlaIII CATG 180 176 . . 177 180 + CviAII CATG 177 179 . . 180 190 + Hin4I GAYNNNNNVTC 171 166 203 198 184 194 + MwoI GCNNNNNNNGC 190 187 . . 186 191 + XhoII RGATCY 186 190 . . 186 191 + NlaIV GGNNCC 188 188 . . 186 191 + BamHI GGATCC 186 190 . . 186 190 + BinI* GGATC 194 195 . . 187 190 + DpnI GATC 188 188 . . 187 190 + BstKTI GATC 189 187 . . 187 190 + MboI GATC 186 190 . . 184 187 - AciI CCGC 184 186 . . 180 190 - Hin4I GAYNNNNNVTC 203 198 171 166 187 191 - BinI* GGATC 181 182 . . 208 212 + Hpy188I TCNGA 210 209 . . 212 215 + TaiI ACGT 215 211 . . 212 215 + SetI ASST 215 211 . . 212 215 + MaeII ACGT 212 214 . . 216 219 + TspEI AATT 215 219 . . 223 226 + MaeI CTAG 223 225 . . 224 229 + TsoI TARCCA 240 238 . . 225 228 + CviJI RGCY 226 226 . . 246 249 + TspEI AATT 245 249 . . 251 254 + CviJI RGCY 252 252 . . 255 258 + DpnI GATC 256 256 . . 255 258 + BstKTI GATC 257 255 . . 255 258 + MboI GATC 254 258 . . 255 259 - BinI* GGATC 249 250 . . 260 263 + TspEI AATT 259 263 . . 265 270 + MmeI TCCRAC 290 288 . . 271 274 + DpnI GATC 272 272 . . 271 274 + BstKTI GATC 273 271 . . 271 280 + BsaBI GATNNNNATC 275 275 . . 271 274 + MboI GATC 270 274 . . 280 284 + DdeI CTNAG 280 283 . . 285 288 + SetI ASST 288 284 . . 285 288 + CviJI RGCY 286 286 . . 285 288 + AluI AGCT 286 286 . . 290 293 + MseI TTAA 290 292 . . 314 317 + MseI TTAA 314 316 . . 317 321 + TspDTI ATGAA 332 330 . . 330 333 + SetI ASST 333 329 . . 344 347 + SetI ASST 347 343 . . 344 347 + CviJI RGCY 345 345 . . 344 347 + AluI AGCT 345 345 . . 345 350 + Cac8I GCNNGC 347 347 . . 348 353 + EciI GGCGGA 364 362 . . 349 352 - AciI CCGC 349 351 . . 359 362 + CviJI RGCY 360 360 . . 357 360 - MnlI CCTC 350 349 . . 360 370 + MwoI GCNNNNNNNGC 366 363 . . 368 371 + SetI ASST 371 367 . . 368 371 + CviJI RGCY 369 369 . . 368 371 + AluI AGCT 369 369 . . 377 380 + TaqI TCGA 377 379 . . 386 390 + FokI GGATG 399 403 . . 386 390 + BseGI GGATG 392 390 . . 392 396 + BslFI GGGAC 406 410 . . 398 401 + SetI ASST 401 397 . . 401 404 + CviRI* TGCA 402 402 . . 402 406 + SfaNI GCATC 411 415 . . 405 409 + Hpy188I TCNGA 407 406 . . 411 415 + FokI GGATG 424 428 . . 411 415 + BseGI GGATG 417 415 . . 408 412 - Hin4II* CCTTC 402 401 . . 414 419 + BclI TGATCA 414 418 . . 415 418 + DpnI GATC 416 416 . . 415 418 + BstKTI GATC 417 415 . . 415 418 + MboI GATC 414 418 . . 443 447 + TspDTI ATGAA 458 456 . . 449 453 + BccI CCATC 457 458 . . 452 456 + Hpy188I TCNGA 454 453 . . 467 471 - FokI GGATG 453 457 . . 467 471 - BseGI GGATG 466 464 . . 476 479 + TaiI ACGT 479 475 . . 476 479 + SetI ASST 479 475 . . 476 479 + MaeII ACGT 476 478 . . 487 490 - MnlI CCTC 480 479 . . 490 494 + MboII GAAGA 502 501 . . 499 503 + Hpy188I TCNGA 501 500 . . 500 504 + DdeI CTNAG 500 503 . . 503 506 + SetI ASST 506 502 . . 503 506 + CviJI RGCY 504 504 . . 503 506 + AluI AGCT 504 504 . . 500 504 - BspCNI CTCAG 492 490 . . 500 504 - BseMII CTCAG 491 489 . . 504 508 + BlsI GCNGC 506 505 . . 504 508 + BisI GCNGC 505 506 . . 504 508 + TseI GCWGC 504 507 . . 504 508 - BbvI GCAGC 491 495 . . 521 526 + SplI* CGTACG 521 525 . . 522 525 + Csp6I GTAC 522 524 . . 522 525 + RsaI GTAC 523 523 . . 519 523 - BceAI ACGGC 504 506 . . 529 534 + Hpy166II GTNNAC 531 531 . . 529 534 + HpaI GTTAAC 531 531 . . 529 534 + HindII GTYRAC 531 531 . . 530 533 + MseI TTAA 530 532 . . 533 536 + TaiI ACGT 536 532 . . 533 536 + SetI ASST 536 532 . . 533 536 + MaeII ACGT 533 535 . . 533 543 + BsaXI ACNNNNNCTCC 523 520 553 550 537 540 + MnlI CCTC 547 546 . . 547 556 + MslI CAYNNNNRTG 551 551 . . 550 555 + ClaI ATCGAT 551 553 . . 551 554 + TaqI TCGA 551 553 . . 571 574 + MaeI CTAG 571 573 . . 573 576 + SetI ASST 576 572 . . 573 576 + CviJI RGCY 574 574 . . 573 576 + AluI AGCT 574 574 . . 575 580 + Eco57MI CTGRAG 596 594 . . 575 580 + Eco57I CTGAAG 596 594 . . 577 581 + MboII GAAGA 589 588 . . 582 585 + TspEI AATT 581 585 . . 587 590 + TaiI ACGT 590 586 . . 587 590 + SetI ASST 590 586 . . 587 590 + MaeII ACGT 587 589 . . 597 600 + SetI ASST 600 596 . . 597 600 + CviJI RGCY 598 598 . . 597 600 + AluI AGCT 598 598 . . 598 602 + BlsI GCNGC 600 599 . . 598 602 + BisI GCNGC 599 600 . . 598 602 + TseI GCWGC 598 601 . . 601 605 + BlsI GCNGC 603 602 . . 601 605 + BisI GCNGC 602 603 . . 601 605 + TseI GCWGC 601 604 . . 598 602 - BbvI GCAGC 585 589 . . 599 604 - EcoP15I CAGCAG 571 573 . . 601 605 - BbvI GCAGC 588 592 . . 630 633 + MnlI CCTC 640 639 . . 632 635 + TaqI TCGA 632 634 . . 645 648 + CviJI RGCY 646 646 . . 646 651 + Cac8I GCNNGC 648 648 . . 646 651 + BmtI GCTAGC 650 646 . . 646 651 + NheI GCTAGC 646 650 . . 647 650 + MaeI CTAG 647 649 . . 644 649 - TsoI TARCCA 634 632 . . 649 652 + SetI ASST 652 648 . . 649 652 + CviJI RGCY 650 650 . . 649 652 + AluI AGCT 650 650 . . 651 656 + SmlI CTYRAG 651 655 . . 651 656 + AflII CTTAAG 651 655 . . 652 655 + MseI TTAA 652 654 . . 673 676 + TaqI TCGA 673 675 . . 675 679 + TfiI GAWTC 675 678 . . 675 679 + HinfI GANTC 675 678 . . 677 681 - TspDTI ATGAA 667 665 . . 703 706 + SetI ASST 706 702 . . 708 713 + TatI WGTACW 708 712 . . 709 712 + Csp6I GTAC 709 711 . . 709 712 + RsaI GTAC 710 710 . . 713 716 + MseI TTAA 713 715 . . 717 721 + DdeI CTNAG 717 720 . . 717 721 + BspCNI CTCAG 730 728 . . 717 721 + BseMII CTCAG 731 729 . . 718 722 + Hpy188I TCNGA 720 719 . . 721 725 - SfaNI GCATC 711 715 . . 725 729 + Hpy99I CGWCG 729 724 . . 724 728 - HgaI GACGC 713 718 . . 752 763 + BstXI CCANNNNNNTGG 759 755 . . 753 762 + MslI CAYNNNNRTG 757 757 . . 784 788 + DdeI CTNAG 784 787 . . 787 790 + CviJI RGCY 788 788 . . 803 806 + TspEI AATT 802 806 . . 812 815 + SetI ASST 815 811 . . 813 817 + HphI GGTGA 825 824 . . 816 820 + TfiI GAWTC 816 819 . . 816 820 + HinfI GANTC 816 819 . . 820 824 + BccI CCATC 828 829 . . 832 840 + AlwNI CAGNNNCTG 837 834 . . 833 838 + XhoII RGATCY 833 837 . . 834 844 + Hin4I GAYNNNNNVTC 825 820 857 852 834 837 + DpnI GATC 835 835 . . 834 837 + BstKTI GATC 836 834 . . 834 837 + MboI GATC 833 837 . . 836 842 + PfoI TCCNGGA 836 841 . . 837 841 + BssKI CCNGG 836 841 . . 837 841 + BseBI CCWGG 838 839 . . 837 841 + ScrFI CCNGG 838 839 . . 837 841 + EcoRII CCWGG 836 841 . . 834 838 - BinI* GGATC 828 829 . . 840 845 + XhoII RGATCY 840 844 . . 840 845 + NlaIV GGNNCC 842 842 . . 840 845 + BamHI GGATCC 840 844 . . 840 844 + BinI* GGATC 848 849 . . 841 844 + DpnI GATC 842 842 . . 841 844 + BstKTI GATC 843 841 . . 841 844 + MboI GATC 840 844 . . 834 844 - Hin4I GAYNNNNNVTC 857 852 825 820 841 845 - BinI* GGATC 835 836 . . 847 857 + MwoI GCNNNNNNNGC 853 850 . . 847 857 + BstAPI GCANNNNNTGC 853 850 . . 851 856 + NdeI CATATG 852 854 . . 854 859 + EcoT22I ATGCAT 858 854 . . 855 858 + CviRI* TGCA 856 856 . . 891 894 + TspEI AATT 890 894 . . 898 903 + ApoI RAATTY 898 902 . . 899 902 + TspEI AATT 898 902 . . 909 913 - Hin4II* CCTTC 903 902 . . 920 923 + CviJI RGCY 921 921 . . 937 941 + TspDTI ATGAA 952 950 . . 941 946 + ApoI RAATTY 941 945 . . 942 945 + TspEI AATT 941 945 . . 947 950 + MseI TTAA 947 949 . . 964 969 + AhaIII* TTTAAA 966 966 . . 965 968 + MseI TTAA 965 967 . . 988 991 - MnlI CCTC 981 980 . . 993 996 + TspEI AATT 992 996 . . 1003 1008 + MfeI CAATTG 1003 1007 . . 1004 1007 + TspEI AATT 1003 1007 . . 1012 1016 + MboII GAAGA 1024 1023 . . 1012 1017 + BbvII* GAAGAC 1019 1023 . . 1012 1021 + XmnI GAANNNNTTC 1016 1016 . . 1016 1019 + TaiI ACGT 1019 1015 . . 1016 1019 + SetI ASST 1019 1015 . . 1016 1019 + MaeII ACGT 1016 1018 . . 1023 1027 + Tsp4CI* ACNGT 1025 1024 . . 1027 1030 + TaqI TCGA 1027 1029 . . 1027 1032 + Hpy178III* TCNNGA 1028 1030 . . 1032 1035 + SetI ASST 1035 1031 . . 1029 1033 - BsmAI GTCTC 1023 1027 . . 1029 1034 - Eco31I GGTCTC 1023 1027 . . 1044 1049 + ApoI RAATTY 1044 1048 . . 1045 1048 + TspEI AATT 1044 1048 . . 1051 1056 + AsuII TTCGAA 1052 1054 . . 1052 1055 + TaqI TCGA 1052 1054 . . 1054 1058 + MboII GAAGA 1066 1065 . . 1056 1061 + XhoII RGATCY 1056 1060 . . 1057 1060 + DpnI GATC 1058 1058 . . 1057 1060 + BstKTI GATC 1059 1057 . . 1057 1060 + MboI GATC 1056 1060 . . 1057 1061 - BinI* GGATC 1051 1052 . . 1072 1076 - BccI CCATC 1066 1067 . . 1114 1117 + MaeI CTAG 1114 1116 . . 1117 1122 + Hpy166II GTNNAC 1119 1119 . . 1117 1122 + BssNAI GTATAC 1119 1119 . . 1117 1122 + AccI GTMKAC 1118 1120 . . 1126 1130 + TfiI GAWTC 1126 1129 . . 1126 1130 + HinfI GANTC 1126 1129 . . 1143 1146 + CviJI RGCY 1144 1144 . . 1148 1151 + MaeI CTAG 1148 1150 . . 1152 1155 + FatI CATG 1151 1155 . . 1152 1155 + NlaIII CATG 1155 1151 . . 1152 1155 + CviAII CATG 1152 1154 . . 1155 1159 + PleI GAGTC 1163 1164 . . 1155 1159 + MlyI GAGTC 1164 1164 . . 1155 1159 + HinfI GANTC 1155 1158 . . 1158 1163 + Hpy178III* TCNNGA 1159 1161 . . 1158 1163 + BetI* WCCGGW 1158 1162 . . 1158 1163 + BspMII* TCCGGA 1158 1162 . . 1159 1162 + HpaII CCGG 1159 1161 . . 1162 1167 + ZraI GACGTC 1164 1164 . . 1162 1167 + AcyI GRCGYC 1163 1165 . . 1162 1167 + AatII GACGTC 1166 1162 . . 1163 1166 + TaiI ACGT 1166 1162 . . 1163 1166 + SetI ASST 1166 1162 . . 1163 1166 + MaeII ACGT 1163 1165 . . 1166 1171 + Hpy178III* TCNNGA 1167 1169 . . 1173 1176 + SetI ASST 1176 1172 . . 1173 1176 + CviJI RGCY 1174 1174 . . 1173 1176 + AluI AGCT 1174 1174 . . 1174 1184 + MwoI GCNNNNNNNGC 1180 1177 . . 1179 1183 + BsrI ACTGG 1184 1182 . . 1182 1185 + CviJI RGCY 1183 1183 . . 1183 1187 + BlsI GCNGC 1185 1184 . . 1183 1187 + BisI GCNGC 1184 1185 . . 1183 1187 + TseI GCWGC 1183 1186 . . 1186 1189 + Hin6I GCGC 1186 1188 . . 1186 1189 + GlaI GCGC 1187 1187 . . 1186 1189 + HhaI GCGC 1188 1186 . . 1183 1187 - BbvI GCAGC 1170 1174 . . 1188 1192 - BceAI ACGGC 1173 1175 . . 1209 1212 - MnlI CCTC 1202 1201 . . 1212 1217 - BsmI GAATGC 1212 1210 . . 1225 1228 + MaeI CTAG 1225 1227 . . 1232 1235 + FatI CATG 1231 1235 . . 1232 1235 + NlaIII CATG 1235 1231 . . 1232 1235 + CviAII CATG 1232 1234 . . 1245 1250 + Hpy178III* TCNNGA 1246 1248 . . 1248 1252 + FokI GGATG 1261 1265 . . 1248 1252 + BseGI GGATG 1254 1252 . . 1249 1259 + Hin4I GAYNNNNNVTC 1240 1235 1272 1267 1249 1253 - SfaNI GCATC 1239 1243 . . 1253 1257 + DdeI CTNAG 1253 1256 . . 1258 1262 + Hpy188I TCNGA 1260 1259 . . 1249 1259 - Hin4I GAYNNNNNVTC 1272 1267 1240 1235 1269 1272 + SetI ASST 1272 1268 . . 1275 1278 + SetI ASST 1278 1274 . . 1277 1281 + DdeI CTNAG 1277 1280 . . 1277 1281 - BspCNI CTCAG 1269 1267 . . 1277 1281 - BseMII CTCAG 1268 1266 . . 1282 1286 + Tsp4CI* ACNGT 1284 1283 . . 1279 1283 - BsmAI GTCTC 1273 1277 . . 1279 1284 - Esp3I CGTCTC 1273 1277 . . 1302 1307 + EcoRI GAATTC 1302 1306 . . 1302 1307 + ApoI RAATTY 1302 1306 . . 1303 1306 + TspEI AATT 1302 1306 . . 1312 1315 + MnlI CCTC 1322 1321 . . 1333 1338 + Hpy166II GTNNAC 1335 1335 . . 1346 1349 + MseI TTAA 1346 1348 . . 1357 1360 + SetI ASST 1360 1356 . . 1365 1368 + SetI ASST 1368 1364 . . 1366 1369 + MnlI CCTC 1376 1375 . . 1374 1378 + Tsp4CI* ACNGT 1376 1375 . . 1388 1391 + TspEI AATT 1387 1391 . . 1408 1413 + ApoI RAATTY 1408 1412 . . 1409 1412 + TspEI AATT 1408 1412 . . 1424 1428 + BceAI ACGGC 1440 1442 . . 1426 1429 + CviJI RGCY 1427 1427 . . 1426 1429 + HaeIII GGCC 1427 1427 . . 1428 1431 + MnlI CCTC 1438 1437 . . 1431 1435 + DdeI CTNAG 1431 1434 . . 1431 1435 + BspCNI CTCAG 1444 1442 . . 1431 1435 + BseMII CTCAG 1445 1443 . . 1432 1436 + Hpy188I TCNGA 1434 1433 . . 1435 1439 + PleI GAGTC 1443 1444 . . 1435 1439 + MlyI GAGTC 1444 1444 . . 1435 1439 + HinfI GANTC 1435 1438 . . 1437 1442 + SalI GTCGAC 1437 1441 . . 1437 1442 + Hpy166II GTNNAC 1439 1439 . . 1437 1442 + HindII GTYRAC 1439 1439 . . 1437 1442 + AccI GTMKAC 1438 1440 . . 1438 1441 + TaqI TCGA 1438 1440 . . 1450 1455 + BsaAI YACGTR 1452 1452 . . 1451 1454 + TaiI ACGT 1454 1450 . . 1451 1454 + SetI ASST 1454 1450 . . 1451 1454 + MaeII ACGT 1451 1453 . . 1456 1460 + TspDTI ATGAA 1471 1469 . . 1458 1462 + MboII GAAGA 1470 1469 . . 1458 1467 + XmnI GAANNNNTTC 1462 1462 . . 1466 1470 + Hpy188I TCNGA 1468 1467 . . 1484 1487 + TspEI AATT 1483 1487 . . 1492 1495 + SetI ASST 1495 1491 . . 1512 1516 + Hpy188I TCNGA 1514 1513 . . 1503 1514 - BcgI CGANNNNNNTGC 1526 1524 1492 1490 1515 1520 + ApoI RAATTY 1515 1519 . . 1516 1519 + TspEI AATT 1515 1519 . . 1529 1534 + SspI AATATT 1531 1531 . . 1535 1540 + MmeI TCCRAC 1560 1558 . . 1549 1554 + EcoP15I CAGCAG 1579 1581 . . 1551 1555 + BlsI GCNGC 1553 1552 . . 1551 1555 + BisI GCNGC 1552 1553 . . 1551 1555 + BbvI GCAGC 1563 1567 . . 1551 1555 + TseI GCWGC 1551 1554 . . 1576 1586 + MwoI GCNNNNNNNGC 1582 1579 . . 1581 1586 + GsaI CCCAGC 1585 1581 . . 1581 1586 + BseYI CCCAGC 1581 1585 . . 1583 1588 + EcoP15I CAGCAG 1613 1615 . . 1585 1589 + BlsI GCNGC 1587 1586 . . 1585 1589 + BisI GCNGC 1586 1587 . . 1585 1589 + BbvI GCAGC 1597 1601 . . 1585 1589 + TseI GCWGC 1585 1588 . . 1587 1592 + HaeII RGCGCY 1591 1587 . . 1588 1591 + Hin6I GCGC 1588 1590 . . 1588 1591 + GlaI GCGC 1589 1589 . . 1588 1591 + HhaI GCGC 1590 1588 . . 1602 1612 + BsiYI* CCNNNNNNNGG 1608 1605 . . 1617 1622 + PsiI TTATAA 1619 1619 . . 1622 1627 + ApoI RAATTY 1622 1626 . . 1623 1626 + TspEI AATT 1622 1626 . . 1640 1643 + MaeI CTAG 1640 1642 . . 1646 1649 + MaeI CTAG 1646 1648 . . 1650 1655 + AsuII TTCGAA 1651 1653 . . 1651 1654 + TaqI TCGA 1651 1653 . . 1673 1680 + SwaI ATTTAAAT 1676 1676 . . 1674 1679 + AhaIII* TTTAAA 1676 1676 . . 1675 1678 + MseI TTAA 1675 1677 . . 1677 1682 + ApoI RAATTY 1677 1681 . . 1678 1681 + TspEI AATT 1677 1681 . . 1681 1684 + MseI TTAA 1681 1683 . . 1682 1687 + TsoI TARCCA 1698 1696 . . 1696 1701 + TaqII CACCCA 1712 1710 . . 1713 1716 + CviJI RGCY 1714 1714 . . 1713 1716 + HaeIII GGCC 1714 1714 . . 1735 1739 + TfiI GAWTC 1735 1738 . . 1735 1739 + HinfI GANTC 1735 1738 . . 1735 1744 + XmnI GAANNNNTTC 1739 1739 . . 1740 1743 + TspEI AATT 1739 1743 . . 1743 1748 + Hpy178III* TCNNGA 1744 1746 . . 1755 1759 + Tsp45I GTSAC 1754 1759 . . 1755 1759 + MaeIII GTNAC 1754 1759 . . 1766 1770 + TspDTI ATGAA 1781 1779 . . 1772 1777 + SplI* CGTACG 1772 1776 . . 1773 1776 + Csp6I GTAC 1773 1775 . . 1773 1776 + RsaI GTAC 1774 1774 . . 1783 1786 + MseI TTAA 1783 1785 . . 1803 1807 - Hin4II* CCTTC 1797 1796 . . 1813 1816 + DpnI GATC 1814 1814 . . 1813 1816 + BstKTI GATC 1815 1813 . . 1813 1816 + MboI GATC 1812 1816 . . 1819 1824 + Hpy178III* TCNNGA 1820 1822 . . 1823 1826 + DpnI GATC 1824 1824 . . 1823 1826 + BstKTI GATC 1825 1823 . . 1823 1826 + MboI GATC 1822 1826 . . 1832 1836 + FauI CCCGC 1840 1842 . . 1833 1836 + AciI CCGC 1833 1835 . . 1835 1839 + TauI GCSGC 1838 1835 . . 1835 1839 + BlsI GCNGC 1837 1836 . . 1835 1839 + BisI GCNGC 1836 1837 . . 1836 1839 + AciI CCGC 1836 1838 . . 1849 1852 + MnlI CCTC 1859 1858 . . 1849 1855 + SauI* CCTNAGG 1850 1853 . . 1850 1854 + DdeI CTNAG 1850 1853 . . 1850 1854 + BspCNI CTCAG 1863 1861 . . 1850 1854 + BseMII CTCAG 1864 1862 . . 1854 1860 + DraII RGGNCCY 1855 1858 . . 1854 1859 + NlaIV GGNNCC 1856 1856 . . 1855 1859 + BmgT120I GGNCC 1856 1857 . . 1855 1859 + AsuI* GGNCC 1855 1858 . . 1856 1859 + CviJI RGCY 1857 1857 . . 1856 1859 + HaeIII GGCC 1857 1857 . . 1858 1863 + StyI CCWWGG 1858 1862 . . 1858 1863 + SecI* CCNNGG 1858 1862 . . 1858 1863 + AvrII CCTAGG 1858 1862 . . 1859 1862 + MaeI CTAG 1859 1861 . . 1871 1876 + BspLU11I* ACATGT 1871 1875 . . 1871 1876 + NspI RCATGY 1875 1871 . . 1871 1876 + AflIII ACRYGT 1871 1875 . . 1872 1875 + FatI CATG 1871 1875 . . 1872 1875 + NlaIII CATG 1875 1871 . . 1872 1875 + CviAII CATG 1872 1874 . . 1884 1890 + PfoI TCCNGGA 1884 1889 . . 1885 1889 + BssKI CCNGG 1884 1889 . . 1885 1889 + BseBI CCWGG 1886 1887 . . 1885 1889 + ScrFI CCNGG 1886 1887 . . 1885 1889 + EcoRII CCWGG 1884 1889 . . 1885 1895 + BsiYI* CCNNNNNNNGG 1891 1888 . . 1885 1895 + PflMI CCANNNNNTGG 1891 1888 . . 1894 1897 + CviJI RGCY 1895 1895 . . 1904 1908 + Hpy188I TCNGA 1906 1905 . . 1912 1915 + CviJI RGCY 1913 1913 . . 1915 1920 + AhaIII* TTTAAA 1917 1917 . . 1916 1919 + MseI TTAA 1916 1918 . . 1921 1925 + TspDTI ATGAA 1936 1934 . . 1923 1928 + ApoI RAATTY 1923 1927 . . 1924 1927 + TspEI AATT 1923 1927 . . 1931 1934 + CviJI RGCY 1932 1932 . . #--------------------------------------- # # # AatII: # [GACGTC] # 772 1166 # # AccI: # [GTMKAC] # 119 320 500 999 # # AciI: # [CCGC] # 3 102 165 184 1484 # # AcyI: # [GRCGYC] # 775 1163 # # AflII: # [CTTAAG] # 651 1287 # # AflIII: # [ACRYGT] # 67 1871 # # AhaIII*: # [TTTAAA] # 21 113 241 710 853 # # AluI: # [AGCT] # 24 24 52 59 70 135 # 286 524 764 # # AlwNI: # [CAGNNNCTG] # 837 1101 # # ApoI: # [RAATTY] # 15 43 55 103 106 107 # 107 108 246 258 790 # # AsuI*: # [GGNCC] # 83 1855 # # AsuII: # [TTCGAA] # 287 599 1052 # # AvaI: # [CYCGRG] # 12 1926 # # AvrII: # [CCTAGG] # 80 1858 # # BamHI: # [GGATCC] # 186 654 1098 # # BbvI: # [GCAGC] # 3 34 94 341 393 491 # 582 # # BbvII*: # [GAAGAC] # 919 1019 # # BccI: # [CCATC] # 238 371 457 872 # # BceAI: # [ACGGC] # 267 498 504 669 # # BcgI: # [CGANNNNNNTGC] # 34 412 1492 # # BciVI: # [GTATCC] # 10 1928 # # BclI: # [TGATCA] # 414 1524 # # BetI*: # [WCCGGW] # 780 1158 # # BinI*: # [GGATC] # 7 13 13 24 55 157 # 203 579 887 # # BisI: # [GCNGC] # 3 34 94 102 250 368 # 505 582 # # BlsI: # [GCNGC] # 3 34 94 101 250 368 # 506 582 # # BmeT110I: # [CYCGRG] # 13 1925 # # BmgT120I: # [GGNCC] # 82 1856 # # BmtI: # [GCTAGC] # 650 1288 # # BsaAI: # [YACGTR] # 486 1452 # # BsaBI: # [GATNNNNATC] # 7 268 1663 # # BsaXI: # [ACNNNNNCTCC] # 30 523 1385 # # BseBI: # [CCWGG] # 52 838 1048 # # BseGI: # [GGATG] # 25 49 392 684 788 # # BseMII: # [CTCAG] # 74 77 177 240 414 419 # 537 # # BseYI: # [CCCAGC] # 154 357 1427 # # BsiYI*: # [CCNNNNNNNGG] # 47 283 1608 # # BslFI: # [GGGAC] # 406 1532 # # BsmAI: # [GTCTC] # 250 665 1023 # # BsmI: # [GAATGC] # 726 1212 # # BspCNI: # [CTCAG] # 75 78 175 238 414 419 # 539 # # BspLU11I*: # [ACATGT] # 67 1871 # # BspMII*: # [TCCGGA] # 780 1158 # # BsrI: # [ACTGG] # 49 754 1135 # # BssKI: # [CCNGG] # 54 836 1048 # # BssNAI: # [GTATAC] # 819 1119 # # BstAPI: # [GCANNNNNTGC] # 853 1085 # # BstKTI: # [GATC] # 7 10 11 16 37 68 # 113 141 144 216 419 756 # # BstXI: # [CCANNNNNNTGG] # 759 1179 # # Cac8I: # [GCNNGC] # 301 347 1290 # # ClaI: # [ATCGAT] # 551 1387 # # Csp6I: # [GTAC] # 52 165 187 470 1064 # # CviAII: # [CATG] # 66 80 177 640 975 # # CviJI: # [RGCY] # 4 6 9 9 15 18 # 19 21 24 26 30 34 # 38 48 59 64 70 133 # 135 138 141 143 223 244 # 287 # # CviRI*: # [TGCA] # 176 226 454 1082 # # DdeI: # [CTNAG] # 18 24 67 68 88 154 # 194 217 220 419 469 # # DpnI: # [GATC] # 7 10 10 16 37 68 # 114 141 144 216 419 756 # # DraII: # [RGGNCCY] # 83 1855 # # EciI: # [GGCGGA] # 364 1574 # # Eco31I: # [GGTCTC] # 915 1023 # # Eco57I: # [CTGAAG] # 596 1342 # # Eco57MI: # [CTGRAG] # 596 1342 # # EcoP15I: # [CAGCAG] # 34 325 571 1008 # # EcoRI: # [GAATTC] # 636 1302 # # EcoRII: # [CCWGG] # 54 836 1048 # # EcoRV: # [GATATC] # 59 1879 # # EcoT22I: # [ATGCAT] # 858 1080 # # Esp3I: # [CGTCTC] # 665 1273 # # FatI: # [CATG] # 67 80 176 640 975 # # FauI: # [CCCGC] # 98 1840 # # FokI: # [GGATG] # 25 29 399 677 808 # # GlaI: # [GCGC] # 349 402 1187 # # GsaI: # [CCCAGC] # 158 353 1427 # # HaeII: # [RGCGCY] # 347 1591 # # HaeIII: # [GGCC] # 81 143 287 1427 # # HgaI: # [GACGC] # 713 1225 # # HhaI: # [GCGC] # 348 402 1188 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 0 0 # 0 0 27 32 32 32 # 32 112 383 622 666 # # Hin4II*: # [CCTTC] # 141 402 501 894 # # Hin6I: # [GCGC] # 350 402 1186 # # HindII: # [GTYRAC] # 499 531 908 # # HinfI: # [GANTC] # 29 127 141 203 280 300 # 310 548 # # HpaI: # [GTTAAC] # 531 1407 # # HpaII: # [CCGG] # 779 1159 # # HphI: # [GGTGA] # 825 1113 # # Hpy166II: # [GTNNAC] # 104 120 216 411 499 588 # # Hpy178III*: # [TCNNGA] # 8 14 76 79 118 131 # 498 1014 # # Hpy188I: # [TCNGA] # 32 34 46 47 47 174 # 197 210 219 392 540 # # Hpy99I: # [CGWCG] # 729 1209 # # MaeI: # [CTAG] # 6 34 76 77 79 213 # 223 348 415 467 # # MaeII: # [ACGT] # 54 57 147 212 264 288 # 429 487 # # MaeIII: # [GTNAC] # 89 184 1665 # # MboI: # [GATC] # 7 8 10 16 37 68 # 116 141 144 216 419 756 # # MboII: # [GAAGA] # 42 87 404 435 468 502 # # MfeI: # [CAATTG] # 935 1003 # # MlyI: # [GAGTC] # 280 494 1164 # # MmeI: # [TCCRAC] # 143 147 378 1270 # # MnlI: # [CCTC] # 54 62 67 79 93 120 # 130 221 341 350 421 # # MseI: # [TTAA] # 6 18 22 24 61 102 # 112 122 133 178 216 234 # 329 381 # # MslI: # [CAYNNNNRTG] # 206 551 1181 # # MwoI: # [GCNNNNNNNGC] # 176 190 327 356 402 487 # # NdeI: # [CATATG] # 852 1086 # # NheI: # [GCTAGC] # 646 1292 # # NlaIII: # [CATG] # 63 80 180 640 975 # # NlaIV: # [GGNNCC] # 82 188 654 1014 # # NspI: # [RCATGY] # 63 1875 # # PflMI: # [CCANNNNNTGG] # 47 1891 # # PfoI: # [TCCNGGA] # 54 836 1048 # # PleI: # [GAGTC] # 280 495 1163 # # PsiI: # [TTATAA] # 319 1619 # # RsaI: # [GTAC] # 53 164 187 470 1064 # # SalI: # [GTCGAC] # 501 1437 # # SauI*: # [CCTNAGG] # 88 1850 # # ScaI: # [AGTACT] # 53 1885 # # ScrFI: # [CCNGG] # 52 838 1048 # # SecI*: # [CCNNGG] # 80 1858 # # SetI: # [ASST] # 6 8 10 10 14 14 # 16 24 27 30 30 40 # 41 45 52 54 73 78 # 82 86 96 109 131 204 # 215 443 # # SfaNI: # [GCATC] # 300 411 528 699 # # SmlI: # [CTYRAG] # 651 1287 # # SplI*: # [CGTACG] # 166 521 1251 # # SspI: # [AATATT] # 407 1531 # # StyI: # [CCWWGG] # 80 1858 # # SwaI: # [ATTTAAAT] # 262 1676 # # TaiI: # [ACGT] # 54 57 147 215 264 288 # 429 484 # # TaqI: # [TCGA] # 25 41 81 174 213 287 # 354 377 386 # # TaqII: # [CACCCA] # 226 1712 # # TatI: # [WGTACW] # 51 657 1230 # # TauI: # [GCSGC] # 100 1838 # # TfiI: # [GAWTC] # 127 141 203 310 548 609 # # TseI: # [GCWGC] # 3 34 94 353 368 504 # 582 # # TsoI: # [TARCCA] # 240 240 394 1064 # # Tsp45I: # [GTSAC] # 184 1754 # # Tsp4CI*: # [ACNGT] # 22 92 259 562 1003 # # TspDTI: # [ATGAA] # 2 126 155 209 285 310 # 332 519 # # TspEI: # [AATT] # 8 11 14 15 21 30 # 32 41 43 51 55 62 # 75 85 88 107 107 108 # 184 221 258 322 # # TspRI: # [CASTGNN] # 129 1809 # # XcmI: # [CCANNNNNNNNNTGG] # 8 143 1787 # # XhoII: # [RGATCY] # 7 186 216 647 882 # # XmnI: # [GAANNNNTTC] # 199 277 446 1016 # # ZraI: # [GACGTC] # 774 1164 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1938 # Reported_sequences: 1 # Reported_hitcount: 481 #---------------------------------------