Restriction Fragments for TDH1/YJL052W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

TDH1/YJL052W on chromosome X from coordinates 338271 to 339269.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.18277.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 2 7 + BclI TGATCA 2 6 . . 3 6 + DpnI GATC 4 4 . . 3 6 + BstKTI GATC 5 3 . . 3 6 + MboI GATC 2 6 . . 5 9 + Hpy188I TCNGA 7 6 . . 9 12 + TspEI AATT 8 12 . . 17 20 + MseI TTAA 17 19 . . 20 24 + Tsp4CI* ACNGT 22 21 . . 32 36 + TfiI GAWTC 32 35 . . 32 36 + HinfI GANTC 32 35 . . 47 52 + Hpy178III* TCNNGA 48 50 . . 48 53 + SmlI CTYRAG 48 52 . . 48 53 + Bce83I* CTTGAG 69 67 . . 57 60 + CviJI RGCY 58 58 . . 62 65 + CviRI* TGCA 63 63 . . 80 83 + SetI ASST 83 79 . . 79 82 - MnlI CCTC 72 71 . . 91 96 + Hpy166II GTNNAC 93 93 . . 91 96 + HindII GTYRAC 93 93 . . 97 100 + DpnI GATC 98 98 . . 97 100 + BstKTI GATC 99 97 . . 97 100 + MboI GATC 96 100 . . 97 101 - BinI* GGATC 91 92 . . 114 125 + BcgI CGANNNNNNTGC 103 101 137 135 121 125 + BlsI GCNGC 123 122 . . 121 125 + BisI GCNGC 122 123 . . 121 125 + TseI GCWGC 121 124 . . 121 125 - BbvI GCAGC 108 112 . . 129 132 + FatI CATG 128 132 . . 129 132 + NlaIII CATG 132 128 . . 129 132 + CviAII CATG 129 131 . . 138 141 + Csp6I GTAC 138 140 . . 138 141 + RsaI GTAC 139 139 . . 142 146 + TfiI GAWTC 142 145 . . 142 146 + HinfI GANTC 142 145 . . 151 154 + FatI CATG 150 154 . . 151 154 + NlaIII CATG 154 150 . . 151 154 + CviAII CATG 151 153 . . 167 170 + Csp6I GTAC 167 169 . . 167 170 + RsaI GTAC 168 168 . . 158 168 - BaeI ACNNNNGTAYC 183 178 150 145 169 173 + Tsp4CI* ACNGT 171 170 . . 178 181 + FatI CATG 177 181 . . 178 181 + NlaIII CATG 181 177 . . 178 181 + CviAII CATG 178 180 . . 202 206 - BccI CCATC 196 197 . . 209 214 + Hpy178III* TCNNGA 210 212 . . 213 216 + DpnI GATC 214 214 . . 213 216 + BstKTI GATC 215 213 . . 213 216 + MboI GATC 212 216 . . 220 223 + SetI ASST 223 219 . . 237 242 + GsaI CCCAGC 241 237 . . 237 242 + BseYI CCCAGC 237 241 . . 233 237 - BsmAI GTCTC 227 231 . . 233 238 - Eco31I GGTCTC 227 231 . . 240 243 + SetI ASST 243 239 . . 240 243 + CviJI RGCY 241 241 . . 240 243 + AluI AGCT 241 241 . . 250 255 + DsaI* CCRYGG 250 254 . . 250 255 + StyI CCWWGG 250 254 . . 250 255 + NcoI CCATGG 250 254 . . 250 255 + SecI* CCNNGG 250 254 . . 251 254 + FatI CATG 250 254 . . 251 254 + NlaIII CATG 254 250 . . 251 254 + CviAII CATG 251 253 . . 267 270 + DpnI GATC 268 268 . . 267 270 + BstKTI GATC 269 267 . . 267 270 + MboI GATC 266 270 . . 268 273 + ClaI ATCGAT 269 271 . . 269 272 + TaqI TCGA 269 271 . . 280 285 + Hpy166II GTNNAC 282 282 . . 280 285 + HindII GTYRAC 282 282 . . 283 287 + HinfI GANTC 283 286 . . 283 287 - PleI GAGTC 277 278 . . 283 287 - MlyI GAGTC 277 277 . . 288 294 + TspRI CASTGNN 294 285 . . 289 293 + BsrI ACTGG 294 292 . . 305 308 + TspEI AATT 304 308 . . 314 317 + AciI CCGC 314 316 . . 314 319 + BsrBI CCGCTC 316 316 . . 331 335 + HgaI GACGC 340 345 . . 337 342 + NlaIV GGNNCC 339 339 . . 337 342 + HgiCI* GGYRCC 337 341 . . 347 350 + SetI ASST 350 346 . . 345 349 - Hin4II* CCTTC 339 338 . . 357 363 + TspRI CASTGNN 363 354 . . 357 362 - BtsI GCAGTG 356 354 . . 364 368 + BccI CCATC 372 373 . . 367 371 - MboII GAAGA 359 358 . . 370 374 - MboII GAAGA 362 361 . . 397 402 + Hpy166II GTNNAC 399 399 . . 397 402 + HpaI GTTAAC 399 399 . . 397 402 + HindII GTYRAC 399 399 . . 398 401 + MseI TTAA 398 400 . . 399 404 + TsoI TARCCA 415 413 . . 417 422 + Hpy178III* TCNNGA 418 420 . . 416 421 - Eco57MI CTGRAG 401 399 . . 416 421 - GsuI CTGGAG 401 399 . . 426 430 + MboII GAAGA 438 437 . . 433 437 + BsmAI GTCTC 438 442 . . 436 441 + MmeI TCCRAC 461 459 . . 449 452 + Csp6I GTAC 449 451 . . 449 452 + RsaI GTAC 450 450 . . 452 466 + XcmI CCANNNNNNNNNTGG 459 458 . . 455 466 + BstXI CCANNNNNNTGG 462 458 . . 458 462 + Tsp4CI* ACNGT 460 459 . . 465 468 + CviJI RGCY 466 466 . . 465 470 + NlaIV GGNNCC 467 467 . . 466 476 + MwoI GCNNNNNNNGC 472 469 . . 473 478 + BalI TGGCCA 475 475 . . 473 478 + CfrI YGGCCR 473 477 . . 474 477 + CviJI RGCY 475 475 . . 474 477 + HaeIII GGCC 475 475 . . 476 481 + StyI CCWWGG 476 480 . . 476 481 + SecI* CCNNGG 476 480 . . 479 482 + SetI ASST 482 478 . . 490 494 - SfaNI GCATC 480 484 . . 505 509 + MboII GAAGA 517 516 . . 510 513 + SetI ASST 513 509 . . 508 512 - Hin4II* CCTTC 502 501 . . 522 528 + TspRI CASTGNN 528 519 . . 523 527 + Tsp4CI* ACNGT 525 524 . . 526 531 + Hpy166II GTNNAC 528 528 . . 534 537 + FatI CATG 533 537 . . 534 537 + NlaIII CATG 537 533 . . 534 537 + CviAII CATG 534 536 . . 537 547 + Hin4I GAYNNNNNVTC 528 523 560 555 539 542 + AciI CCGC 539 541 . . 553 557 + Tsp4CI* ACNGT 555 554 . . 559 569 + Hin4I GAYNNNNNVTC 550 545 582 577 562 566 + BmgT120I GGNCC 563 564 . . 562 566 + AvaII GGWCC 562 565 . . 562 566 + AsuI* GGNCC 562 565 . . 559 563 - BccI CCATC 553 554 . . 565 569 + BccI CCATC 573 574 . . 559 569 - Hin4I GAYNNNNNVTC 582 577 550 545 566 570 - FokI GGATG 552 556 . . 566 570 - BseGI GGATG 565 563 . . 578 582 + BsrI ACTGG 583 581 . . 579 584 + Eco57MI CTGRAG 600 598 . . 579 584 + GsuI CTGGAG 600 598 . . 585 588 + SetI ASST 588 584 . . 584 587 - MnlI CCTC 577 576 . . 581 591 - BsaXI ACNNNNNCTCC 603 600 573 570 593 602 + XmnI GAANNNNTTC 597 597 . . 596 599 + AciI CCGC 596 598 . . 601 606 + BetI* WCCGGW 601 605 . . 602 605 + HpaII CCGG 602 604 . . 590 602 - BarI GAAGNNNNNNTAC 614 609 582 577 605 609 + MaeIII GTNAC 604 609 . . 616 620 + BccI CCATC 624 625 . . 620 623 + MnlI CCTC 630 629 . . 617 621 - FokI GGATG 603 607 . . 617 621 - BseGI GGATG 616 614 . . 625 630 + Cfr10I RCCGGY 625 629 . . 625 630 + BetI* WCCGGW 625 629 . . 625 630 + AgeI ACCGGT 625 629 . . 626 629 + HpaII CCGG 626 628 . . 631 641 + MwoI GCNNNNNNNGC 637 634 . . 631 635 + BlsI GCNGC 633 632 . . 631 635 + BisI GCNGC 632 633 . . 631 635 + TseI GCWGC 631 634 . . 635 639 + DdeI CTNAG 635 638 . . 631 635 - BbvI GCAGC 618 622 . . 639 642 + CviJI RGCY 640 640 . . 634 640 - Bpu10I CCTNAGC 635 638 . . 650 653 + SetI ASST 653 649 . . 657 667 + MwoI GCNNNNNNNGC 663 660 . . 662 665 + TspEI AATT 661 665 . . 665 668 + CviRI* TGCA 666 666 . . 669 672 + SetI ASST 672 668 . . 675 680 + Hpy166II GTNNAC 677 677 . . 675 680 + HindII GTYRAC 677 677 . . 679 684 + Cfr10I RCCGGY 679 683 . . 679 684 + BetI* WCCGGW 679 683 . . 679 684 + AgeI ACCGGT 679 683 . . 680 683 + HpaII CCGG 680 682 . . 687 690 + CviJI RGCY 688 688 . . 692 696 + Hpy188I TCNGA 694 693 . . 695 699 + PleI GAGTC 703 704 . . 695 699 + MlyI GAGTC 704 704 . . 695 699 + HinfI GANTC 695 698 . . 697 701 - BslFI GGGAC 682 686 . . 703 707 + Tsp4CI* ACNGT 705 704 . . 705 709 + Hpy99I CGWCG 709 704 . . 707 710 + TaqI TCGA 707 709 . . 715 719 - TspGWI ACGGA 705 703 . . 721 726 + Hpy166II GTNNAC 723 723 . . 721 726 + HindII GTYRAC 723 723 . . 724 735 + DrdI GACNNNNNNGTC 730 728 . . 730 734 + Tsp4CI* ACNGT 732 731 . . 738 743 - MmeI TCCRAC 719 717 . . 750 753 + SetI ASST 753 749 . . 750 753 + CviJI RGCY 751 751 . . 750 753 + AluI AGCT 751 751 . . 767 772 + Hpy178III* TCNNGA 768 770 . . 774 777 + CviJI RGCY 775 775 . . 771 775 - Hin4II* CCTTC 765 764 . . 775 785 + MwoI GCNNNNNNNGC 781 778 . . 779 782 + MseI TTAA 779 781 . . 783 786 + CviJI RGCY 784 784 . . 784 788 + BlsI GCNGC 786 785 . . 784 788 + BisI GCNGC 785 786 . . 784 788 + TseI GCWGC 784 787 . . 787 791 + TauI GCSGC 790 787 . . 787 791 + BlsI GCNGC 789 788 . . 787 791 + BisI GCNGC 788 789 . . 788 793 + NspBII* CMGCKG 790 790 . . 784 788 - BbvI GCAGC 771 775 . . 788 791 + AciI CCGC 788 790 . . 791 796 + Eco57MI CTGRAG 812 810 . . 791 796 + Eco57I CTGAAG 812 810 . . 795 798 + SetI ASST 798 794 . . 796 800 + BmgT120I GGNCC 797 798 . . 796 800 + AvaII GGWCC 796 799 . . 796 800 + AsuI* GGNCC 796 799 . . 793 797 - Hin4II* CCTTC 787 786 . . 799 809 + BsiYI* CCNNNNNNNGG 805 802 . . 802 806 + TspDTI ATGAA 817 815 . . 804 817 + AjuI GAANNNNNNNTTGG 796 791 828 823 804 808 - Hin4II* CCTTC 798 797 . . 818 822 + MaeIII GTNAC 817 822 . . 826 830 + MboII GAAGA 838 837 . . 829 833 - SfaNI GCATC 819 823 . . 832 836 - BceAI ACGGC 817 819 . . 838 842 + BsmAI GTCTC 843 847 . . 842 845 + MnlI CCTC 852 851 . . 844 848 + Hpy188I TCNGA 846 845 . . 840 845 - BseRI GAGGAG 831 829 . . 856 860 + HphI GGTGA 868 867 . . 857 861 + Tsp45I GTSAC 856 861 . . 857 861 + MaeIII GTNAC 856 861 . . 854 859 - TaqII CACCCA 844 842 . . 872 876 + BccI CCATC 880 881 . . 878 881 + TaqI TCGA 878 880 . . 875 879 - MboII GAAGA 867 866 . . 884 887 + MnlI CCTC 894 893 . . 880 884 - SfaNI GCATC 870 874 . . 887 892 + NspBII* CMGCKG 889 889 . . 887 890 + AciI CCGC 887 889 . . 893 898 + BciVI GTATCC 904 903 . . 898 903 + MfeI CAATTG 898 902 . . 899 902 + TspEI AATT 898 902 . . 903 907 + BsmAI GTCTC 908 912 . . 929 933 + BssKI CCNGG 928 933 . . 929 933 + BseBI CCWGG 930 931 . . 929 933 + ScrFI CCNGG 930 931 . . 929 933 + EcoRII CCWGG 928 933 . . 933 936 + Csp6I GTAC 933 935 . . 933 936 + RsaI GTAC 934 934 . . 947 951 + Tsp4CI* ACNGT 949 948 . . 947 957 + BsaXI ACNNNNNCTCC 937 934 967 964 950 954 + MaeIII GTNAC 949 954 . . 956 959 + AciI CCGC 956 958 . . 955 960 - EciI GGCGGA 945 943 . . 967 972 + Hpy166II GTNNAC 969 969 . . 967 972 + HindII GTYRAC 969 969 . . 975 978 + DpnI GATC 976 976 . . 975 978 + BstKTI GATC 977 975 . . 975 978 + MboI GATC 974 978 . . 977 980 + TaqI TCGA 977 979 . . 978 989 + BcgI CGANNNNNNTGC 967 965 . . 989 994 + StyI CCWWGG 989 993 . . 989 994 + SecI* CCNNGG 989 993 . . 993 996 + CviJI RGCY 994 994 . . 996 999 + MseI TTAA 996 998 . . #--------------------------------------- # # # AciI: # [CCGC] # 43 57 69 99 192 225 # 314 # # AgeI: # [ACCGGT] # 54 320 625 # # AjuI: # [GAANNNNNNNTTGG] # 32 171 796 # # AluI: # [AGCT] # 241 248 510 # # AsuI*: # [GGNCC] # 203 234 562 # # AvaII: # [GGWCC] # 203 234 562 # # BaeI: # [ACNNNNGTAYC] # 33 150 816 # # BalI: # [TGGCCA] # 475 524 # # BarI: # [GAAGNNNNNNTAC] # 32 385 582 # # BbvI: # [GCAGC] # 108 153 228 510 # # BccI: # [CCATC] # 20 51 119 176 181 196 # 256 # # Bce83I*: # [CTTGAG] # 69 930 # # BceAI: # [ACGGC] # 182 817 # # BcgI: # [CGANNNNNNTGC] # 32 34 103 830 # # BciVI: # [GTATCC] # 95 904 # # BclI: # [TGATCA] # 2 997 # # BetI*: # [WCCGGW] # 24 54 320 601 # # BinI*: # [GGATC] # 91 908 # # BisI: # [GCNGC] # 3 122 153 211 510 # # BlsI: # [GCNGC] # 3 123 153 210 510 # # BmgT120I: # [GGNCC] # 202 234 563 # # Bpu10I: # [CCTNAGC] # 364 635 # # BsaXI: # [ACNNNNNCTCC] # 30 30 32 334 573 # # BseBI: # [CCWGG] # 69 930 # # BseGI: # [GGATG] # 51 383 565 # # BseRI: # [GAGGAG] # 168 831 # # BseYI: # [CCCAGC] # 237 762 # # BsiYI*: # [CCNNNNNNNGG] # 194 805 # # BslFI: # [GGGAC] # 317 682 # # BsmAI: # [GTCTC] # 65 91 211 227 405 # # BsrBI: # [CCGCTC] # 316 683 # # BsrI: # [ACTGG] # 289 294 416 # # BssKI: # [CCNGG] # 71 928 # # BstKTI: # [GATC] # 5 22 54 94 116 708 # # BstXI: # [CCANNNNNNTGG] # 462 537 # # BtsI: # [GCAGTG] # 356 643 # # Cfr10I: # [RCCGGY] # 54 320 625 # # CfrI: # [YGGCCR] # 473 526 # # ClaI: # [ATCGAT] # 269 730 # # Csp6I: # [GTAC] # 29 66 138 282 484 # # CviAII: # [CATG] # 22 27 73 129 283 465 # # CviJI: # [RGCY] # 5 9 9 24 48 58 # 63 165 183 210 225 # # CviRI*: # [TGCA] # 63 333 603 # # DdeI: # [CTNAG] # 364 635 # # DpnI: # [GATC] # 4 23 54 94 116 708 # # DrdI: # [GACNNNNNNGTC] # 269 730 # # DsaI*: # [CCRYGG] # 250 749 # # EciI: # [GGCGGA] # 54 945 # # Eco31I: # [GGTCTC] # 227 772 # # Eco57I: # [CTGAAG] # 187 812 # # Eco57MI: # [CTGRAG] # 187 199 212 401 # # EcoRII: # [CCWGG] # 71 928 # # FatI: # [CATG] # 22 27 73 128 283 466 # # FokI: # [GGATG] # 51 396 552 # # GsaI: # [CCCAGC] # 241 758 # # GsuI: # [CTGGAG] # 199 399 401 # # HaeIII: # [GGCC] # 475 524 # # HgaI: # [GACGC] # 340 659 # # HgiCI*: # [GGYRCC] # 337 662 # # Hin4I: # [GAYNNNNNVTC] # 0 0 10 22 22 417 # 528 # # Hin4II*: # [CCTTC] # 11 22 163 201 263 339 # # HindII: # [GTYRAC] # 30 46 93 117 189 246 # 278 # # HinfI: # [GANTC] # 32 110 141 304 412 # # HpaI: # [GTTAAC] # 399 600 # # HpaII: # [CCGG] # 24 54 319 602 # # HphI: # [GGTGA] # 131 868 # # Hpy166II: # [GTNNAC] # 30 46 93 117 129 149 # 189 246 # # Hpy178III*: # [TCNNGA] # 48 162 208 231 350 # # Hpy188I: # [TCNGA] # 7 152 153 687 # # Hpy99I: # [CGWCG] # 290 709 # # MaeIII: # [GTNAC] # 39 50 93 213 604 # # MboI: # [GATC] # 2 25 54 94 116 708 # # MboII: # [GAAGA] # 3 29 76 79 132 321 # 359 # # MfeI: # [CAATTG] # 101 898 # # MlyI: # [GAGTC] # 277 295 427 # # MmeI: # [TCCRAC] # 258 280 461 # # MnlI: # [CCTC] # 42 53 72 105 222 505 # # MseI: # [TTAA] # 3 17 217 381 381 # # MwoI: # [GCNNNNNNNGC] # 26 118 165 218 472 # # NcoI: # [CCATGG] # 250 749 # # NlaIII: # [CATG] # 22 27 73 132 283 462 # # NlaIV: # [GGNNCC] # 128 339 532 # # NspBII*: # [CMGCKG] # 99 110 790 # # PleI: # [GAGTC] # 277 296 426 # # RsaI: # [GTAC] # 29 65 139 282 484 # # ScrFI: # [CCNGG] # 69 930 # # SecI*: # [CCNNGG] # 10 226 250 513 # # SetI: # [ASST] # 19 20 31 45 65 75 # 81 83 107 132 140 201 # # SfaNI: # [GCATC] # 51 129 339 480 # # SmlI: # [CTYRAG] # 48 951 # # StyI: # [CCWWGG] # 10 226 250 513 # # TaqI: # [TCGA] # 22 99 171 269 438 # # TaqII: # [CACCCA] # 155 844 # # TauI: # [GCSGC] # 209 790 # # TfiI: # [GAWTC] # 32 110 857 # # TseI: # [GCWGC] # 121 153 215 510 # # TsoI: # [TARCCA] # 415 584 # # Tsp45I: # [GTSAC] # 143 856 # # Tsp4CI*: # [ACNGT] # 22 27 30 50 65 149 # 150 217 289 # # TspDTI: # [ATGAA] # 182 817 # # TspEI: # [AATT] # 8 101 237 296 357 # # TspGWI: # [ACGGA] # 294 705 # # TspRI: # [CASTGNN] # 69 165 294 471 # # XcmI: # [CCANNNNNNNNNTGG] # 459 540 # # XmnI: # [GAANNNNTTC] # 402 597 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 999 # Reported_sequences: 1 # Reported_hitcount: 266 #---------------------------------------