Restriction Fragments for BBC1/YJL020C



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The currently selected gene/sequence is :

BBC1/YJL020C on chromosome X from coordinates 402410 to 398937.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.15102.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 5 10 + Hpy166II GTNNAC 7 7 . . 16 21 + SduI GDGCHC 20 16 . . 16 21 + BseSI GKGCMC 20 16 . . 20 24 + Hin4II* CCTTC 30 29 . . 26 29 + SetI ASST 29 25 . . 33 36 + CviJI RGCY 34 34 . . 35 39 + DdeI CTNAG 35 38 . . 35 39 + BspCNI CTCAG 48 46 . . 35 39 + BseMII CTCAG 49 47 . . 52 56 + Hpy188I TCNGA 54 53 . . 61 64 - MnlI CCTC 54 53 . . 68 75 + SwaI ATTTAAAT 71 71 . . 69 74 + AhaIII* TTTAAA 71 71 . . 70 73 + MseI TTAA 70 72 . . 72 77 + ApoI RAATTY 72 76 . . 73 76 + TspEI AATT 72 76 . . 78 89 + BdaI TGANNNNNNTCA 67 65 101 99 85 88 + DpnI GATC 86 86 . . 85 88 + BstKTI GATC 87 85 . . 85 88 + MboI GATC 84 88 . . 87 92 + Hpy178III* TCNNGA 88 90 . . 78 89 - BdaI TGANNNNNNTCA 101 99 67 65 100 104 + MaeIII GTNAC 99 104 . . 103 106 + TaiI ACGT 106 102 . . 103 106 + SetI ASST 106 102 . . 103 106 + MaeII ACGT 103 105 . . 112 116 + MboII GAAGA 124 123 . . 112 117 + BbvII* GAAGAC 119 123 . . 115 119 + HgaI GACGC 124 129 . . 115 120 + AcyI GRCGYC 116 118 . . 121 134 + AjuI GAANNNNNNNTTGG 113 108 145 140 126 129 + Csp6I GTAC 126 128 . . 126 129 + RsaI GTAC 127 127 . . 133 137 + HphI GGTGA 145 144 . . 125 135 - BaeI ACNNNNGTAYC 150 145 117 112 141 155 + XcmI CCANNNNNNNNNTGG 148 147 . . 141 145 + BssKI CCNGG 140 145 . . 141 145 + BseBI CCWGG 142 143 . . 141 145 + ScrFI CCNGG 142 143 . . 141 145 + EcoRII CCWGG 140 145 . . 145 149 + TfiI GAWTC 145 148 . . 145 149 + HinfI GANTC 145 148 . . 156 160 + Hpy99I CGWCG 160 155 . . 158 161 + TaiI ACGT 161 157 . . 158 161 + SetI ASST 161 157 . . 158 161 + MaeII ACGT 158 160 . . 166 169 - MnlI CCTC 159 158 . . 176 181 + Hpy178III* TCNNGA 177 179 . . 173 177 - MboII GAAGA 165 164 . . 202 207 + XhoII RGATCY 202 206 . . 202 206 + BinI* GGATC 210 211 . . 203 206 + DpnI GATC 204 204 . . 203 206 + BstKTI GATC 205 203 . . 203 206 + MboI GATC 202 206 . . 205 209 + Hpy188I TCNGA 207 206 . . 206 211 + Eco57MI CTGRAG 227 225 . . 206 211 + Eco57I CTGAAG 227 225 . . 211 216 - MmeI TCCRAC 192 190 . . 222 225 + CviJI RGCY 223 223 . . 226 230 + TfiI GAWTC 226 229 . . 226 230 + HinfI GANTC 226 229 . . 234 238 + Hpy188I TCNGA 236 235 . . 235 245 + BsiYI* CCNNNNNNNGG 241 238 . . 241 246 + Cfr10I RCCGGY 241 245 . . 241 246 + BetI* WCCGGW 241 245 . . 241 246 + AgeI ACCGGT 241 245 . . 242 245 + HpaII CCGG 242 244 . . 244 249 + NlaIV GGNNCC 246 246 . . 249 255 + TspRI CASTGNN 255 246 . . 251 255 + DdeI CTNAG 251 254 . . 251 255 - BspCNI CTCAG 243 241 . . 251 255 - BseMII CTCAG 242 240 . . 258 261 + TaiI ACGT 261 257 . . 258 261 + SetI ASST 261 257 . . 258 261 + MaeII ACGT 258 260 . . 260 263 + Csp6I GTAC 260 262 . . 260 263 + RsaI GTAC 261 261 . . 269 272 + CviJI RGCY 270 270 . . 271 274 + HpaII CCGG 271 273 . . 287 293 + PpuMI RGGWCCY 288 291 . . 287 293 + DraII RGGNCCY 288 291 . . 288 292 + BmgT120I GGNCC 289 290 . . 288 292 + AvaII GGWCC 288 291 . . 288 292 + AsuI* GGNCC 288 291 . . 290 293 + SetI ASST 293 289 . . 298 303 + SduI GDGCHC 302 298 . . 298 303 + HgiJII* GRGCYC 302 298 . . 299 302 + CviJI RGCY 300 300 . . 318 322 + MboII GAAGA 330 329 . . 335 339 + BssKI CCNGG 334 339 . . 335 338 + HpaII CCGG 335 337 . . 335 339 + ScrFI CCNGG 336 337 . . 335 339 + CauII* CCSGG 336 337 . . 337 341 + BmgT120I GGNCC 338 339 . . 337 341 + AsuI* GGNCC 337 340 . . 338 341 + CviJI RGCY 339 339 . . 338 341 + HaeIII GGCC 339 339 . . 341 347 + TspRI CASTGNN 347 338 . . 340 344 - BsrI ACTGG 340 338 . . 346 350 - BsrI ACTGG 346 344 . . 354 357 + CviJI RGCY 355 355 . . 355 359 + TauI GCSGC 358 355 . . 355 359 + BlsI GCNGC 357 356 . . 355 359 + BisI GCNGC 356 357 . . 356 359 + AciI CCGC 356 358 . . 359 364 + SfeI* CTRYAG 359 363 . . 361 365 + Tsp4CI* ACNGT 363 362 . . 362 368 + TspRI CASTGNN 368 359 . . 367 371 - BsrI ACTGG 367 365 . . 375 378 + CviJI RGCY 376 376 . . 376 380 + TauI GCSGC 379 376 . . 376 380 + BlsI GCNGC 378 377 . . 376 380 + BisI GCNGC 377 378 . . 377 380 + AciI CCGC 377 379 . . 380 385 + SfeI* CTRYAG 380 384 . . 382 386 + Tsp4CI* ACNGT 384 383 . . 383 389 + TspRI CASTGNN 389 380 . . 388 392 - BsrI ACTGG 388 386 . . 396 399 + CviJI RGCY 397 397 . . 397 401 + TauI GCSGC 400 397 . . 397 407 + MwoI GCNNNNNNNGC 403 400 . . 397 401 + BlsI GCNGC 399 398 . . 397 401 + BisI GCNGC 398 399 . . 398 401 + AciI CCGC 398 400 . . 401 406 + SfeI* CTRYAG 401 405 . . 403 413 + BaeI ACNNNNGTAYC 392 387 425 420 404 409 + EcoP15I CAGCAG 434 436 . . 406 416 + MwoI GCNNNNNNNGC 412 409 . . 419 422 + SetI ASST 422 418 . . 420 424 + BmgT120I GGNCC 421 422 . . 420 424 + AvaII GGWCC 420 423 . . 420 424 + AsuI* GGNCC 420 423 . . 427 430 + FatI CATG 426 430 . . 427 430 + NlaIII CATG 430 426 . . 427 430 + CviAII CATG 427 429 . . 464 469 + DsaI* CCRYGG 464 468 . . 464 469 + StyI CCWWGG 464 468 . . 464 469 + NcoI CCATGG 464 468 . . 464 469 + SecI* CCNNGG 464 468 . . 465 468 + FatI CATG 464 468 . . 465 468 + NlaIII CATG 468 464 . . 465 468 + CviAII CATG 465 467 . . 469 473 + TfiI GAWTC 469 472 . . 469 473 + HinfI GANTC 469 472 . . 479 482 + TspEI AATT 478 482 . . 484 494 + FalI AAGNNNNNCTT 475 470 507 502 486 489 + CviJI RGCY 487 487 . . 483 487 - Hin4II* CCTTC 477 476 . . 487 492 + Cac8I GCNNGC 489 489 . . 484 494 - FalI AAGNNNNNCTT 507 502 475 470 503 506 + MseI TTAA 503 505 . . 507 512 + Hpy178III* TCNNGA 508 510 . . 517 521 + TspGWI ACGGA 532 530 . . 564 573 + MslI CAYNNNNRTG 568 568 . . 567 570 + SetI ASST 570 566 . . 581 586 + MfeI CAATTG 581 585 . . 582 585 + TspEI AATT 581 585 . . 592 596 - SfaNI GCATC 582 586 . . 610 614 - BslFI GGGAC 595 599 . . 615 620 + SecI* CCNNGG 615 619 . . 616 620 + BssKI CCNGG 615 620 . . 616 620 + BseBI CCWGG 617 618 . . 616 620 + ScrFI CCNGG 617 618 . . 616 620 + EcoRII CCWGG 615 620 . . 620 625 + EcoRI GAATTC 620 624 . . 620 625 + ApoI RAATTY 620 624 . . 621 624 + TspEI AATT 620 624 . . 624 629 + MmeI TCCRAC 649 647 . . 637 640 + TaiI ACGT 640 636 . . 637 640 + SetI ASST 640 636 . . 637 640 + MaeII ACGT 637 639 . . 645 650 + Hpy178III* TCNNGA 646 648 . . 660 664 - BslFI GGGAC 645 649 . . 690 695 + Hpy178III* TCNNGA 691 693 . . 699 702 + TspEI AATT 698 702 . . 700 705 + VspI ATTAAT 701 703 . . 701 704 + MseI TTAA 701 703 . . 707 712 + Hpy166II GTNNAC 709 709 . . 707 712 + SduI GDGCHC 711 707 . . 707 712 + BseSI GKGCMC 711 707 . . 707 712 + ApaLI GTGCAC 707 711 . . 707 712 + HgiAI* GWGCWC 711 707 . . 708 711 + CviRI* TGCA 709 709 . . 713 716 + SetI ASST 716 712 . . 725 729 + MboII GAAGA 737 736 . . 737 741 + DdeI CTNAG 737 740 . . 737 741 - BspCNI CTCAG 729 727 . . 737 741 - BseMII CTCAG 728 726 . . 749 752 + TspEI AATT 748 752 . . 751 754 + MseI TTAA 751 753 . . 756 760 - Hin4II* CCTTC 750 749 . . 775 778 + CviJI RGCY 776 776 . . 779 782 + MseI TTAA 779 781 . . 789 792 + TspEI AATT 788 792 . . 822 825 + CviJI RGCY 823 823 . . 823 827 + BlsI GCNGC 825 824 . . 823 827 + BisI GCNGC 824 825 . . 823 827 + TseI GCWGC 823 826 . . 825 828 + CviRI* TGCA 826 826 . . 823 827 - BbvI GCAGC 810 814 . . 832 836 + MboII GAAGA 844 843 . . 835 839 + MboII GAAGA 847 846 . . 842 847 + Hpy178III* TCNNGA 843 845 . . 843 848 + SmlI CTYRAG 843 847 . . 843 848 + Bce83I* CTTGAG 864 862 . . 855 858 + CviJI RGCY 856 856 . . 856 862 + EspI* GCTNAGC 857 860 . . 857 861 + DdeI CTNAG 857 860 . . 860 863 + SetI ASST 863 859 . . 860 863 + CviJI RGCY 861 861 . . 860 863 + AluI AGCT 861 861 . . 863 866 + TaqI TCGA 863 865 . . 874 877 + FatI CATG 873 877 . . 874 877 + NlaIII CATG 877 873 . . 874 877 + CviAII CATG 874 876 . . 875 879 + TspDTI ATGAA 890 888 . . 879 882 + TaiI ACGT 882 878 . . 879 882 + SetI ASST 882 878 . . 879 882 + MaeII ACGT 879 881 . . 889 894 + Hpy166II GTNNAC 891 891 . . 889 894 + HpaI GTTAAC 891 891 . . 889 894 + HindII GTYRAC 891 891 . . 886 889 - AciI CCGC 886 888 . . 890 893 + MseI TTAA 890 892 . . 901 906 + SduI GDGCHC 905 901 . . 901 906 + HgiJII* GRGCYC 905 901 . . 902 905 + CviJI RGCY 903 903 . . 910 914 + TspGWI ACGGA 925 923 . . 917 922 + Eco57MI CTGRAG 938 936 . . 917 922 + Eco57I CTGAAG 938 936 . . 919 923 + MboII GAAGA 931 930 . . 928 932 + MboII GAAGA 940 939 . . 935 939 + TspDTI ATGAA 950 948 . . 954 957 + SetI ASST 957 953 . . 958 963 + ApoI RAATTY 958 962 . . 959 962 + TspEI AATT 958 962 . . 974 978 + DdeI CTNAG 974 977 . . 976 981 + SduI GDGCHC 980 976 . . 976 981 + HgiAI* GWGCWC 980 976 . . 974 978 - BspCNI CTCAG 966 964 . . 974 978 - BseMII CTCAG 965 963 . . 987 992 + NlaIV GGNNCC 989 989 . . 985 988 - MnlI CCTC 978 977 . . 991 994 + AciI CCGC 991 993 . . 1001 1004 + TspEI AATT 1000 1004 . . 1030 1033 + SetI ASST 1033 1029 . . 1031 1034 + MnlI CCTC 1041 1040 . . 1036 1039 + FnuDII* CGCG 1037 1037 . . 1046 1050 + TspDTI ATGAA 1061 1059 . . 1048 1052 - Hin4II* CCTTC 1042 1041 . . 1076 1079 + TspEI AATT 1075 1079 . . 1088 1093 + TatI WGTACW 1088 1092 . . 1089 1094 + Hpy166II GTNNAC 1091 1091 . . 1089 1092 + Csp6I GTAC 1089 1091 . . 1089 1092 + RsaI GTAC 1090 1090 . . 1104 1108 + Hpy188I TCNGA 1106 1105 . . 1114 1119 + BseRI GAGGAG 1129 1127 . . 1117 1121 + PleI GAGTC 1125 1126 . . 1114 1117 - MnlI CCTC 1107 1106 . . 1117 1121 + MlyI GAGTC 1126 1126 . . 1117 1121 + HinfI GANTC 1117 1120 . . 1119 1123 + Tsp45I GTSAC 1118 1123 . . 1119 1123 + MaeIII GTNAC 1118 1123 . . 1126 1136 + MwoI GCNNNNNNNGC 1132 1129 . . 1132 1135 - MnlI CCTC 1125 1124 . . 1139 1144 + BseRI GAGGAG 1154 1152 . . 1139 1142 - MnlI CCTC 1132 1131 . . 1141 1151 - BsaXI ACNNNNNCTCC 1163 1160 1133 1130 1154 1158 + DdeI CTNAG 1154 1157 . . 1154 1158 - BspCNI CTCAG 1146 1144 . . 1154 1158 - BseMII CTCAG 1145 1143 . . 1156 1159 - MnlI CCTC 1149 1148 . . 1153 1159 - Bpu10I CCTNAGC 1154 1157 . . 1153 1159 - BbvCI CCTCAGC 1154 1157 . . 1170 1173 + SetI ASST 1173 1169 . . 1172 1176 + MaeIII GTNAC 1171 1176 . . 1175 1179 + BceAI ACGGC 1191 1193 . . 1176 1181 + CfrI YGGCCR 1176 1180 . . 1177 1180 + CviJI RGCY 1178 1178 . . 1177 1180 + HaeIII GGCC 1178 1178 . . 1180 1183 + FatI CATG 1179 1183 . . 1180 1183 + NlaIII CATG 1183 1179 . . 1180 1183 + CviAII CATG 1180 1182 . . 1181 1185 + TspDTI ATGAA 1196 1194 . . 1183 1187 + MboII GAAGA 1195 1194 . . 1183 1188 + BbvII* GAAGAC 1190 1194 . . 1192 1195 - MnlI CCTC 1185 1184 . . 1207 1210 - MnlI CCTC 1200 1199 . . 1216 1220 + MboII GAAGA 1228 1227 . . 1216 1221 - Ksp632I* CTCTTC 1211 1214 . . 1219 1222 - MnlI CCTC 1212 1211 . . 1222 1226 + MboII GAAGA 1234 1233 . . 1231 1235 + MboII GAAGA 1243 1242 . . 1236 1240 + TfiI GAWTC 1236 1239 . . 1236 1240 + HinfI GANTC 1236 1239 . . 1231 1236 - Ksp632I* CTCTTC 1226 1229 . . 1244 1248 + BmgT120I GGNCC 1245 1246 . . 1244 1248 + AsuI* GGNCC 1244 1247 . . 1245 1248 + CviJI RGCY 1246 1246 . . 1245 1248 + HaeIII GGCC 1246 1246 . . 1246 1250 + TauI GCSGC 1249 1246 . . 1246 1250 + BlsI GCNGC 1248 1247 . . 1246 1250 + BisI GCNGC 1247 1248 . . 1247 1250 + AciI CCGC 1247 1249 . . 1266 1269 + CviJI RGCY 1267 1267 . . 1265 1270 - TsoI TARCCA 1255 1253 . . 1279 1284 + HaeII RGCGCY 1283 1279 . . 1280 1283 + Hin6I GCGC 1280 1282 . . 1280 1283 + GlaI GCGC 1281 1281 . . 1280 1283 + HhaI GCGC 1282 1280 . . 1283 1286 + MaeI CTAG 1283 1285 . . 1289 1293 + TfiI GAWTC 1289 1292 . . 1289 1293 + HinfI GANTC 1289 1292 . . 1295 1300 + SduI GDGCHC 1299 1295 . . 1295 1300 + HgiAI* GWGCWC 1299 1295 . . 1310 1313 + MseI TTAA 1310 1312 . . 1316 1320 + Hin4II* CCTTC 1326 1325 . . 1326 1329 + CviJI RGCY 1327 1327 . . 1330 1335 + Hpy178III* TCNNGA 1331 1333 . . 1331 1336 + Eco57MI CTGRAG 1352 1350 . . 1331 1336 + GsuI CTGGAG 1352 1350 . . 1336 1341 - MmeI TCCRAC 1317 1315 . . 1348 1352 + BccI CCATC 1356 1357 . . 1351 1355 + Hpy188I TCNGA 1353 1352 . . 1354 1358 + MboII GAAGA 1366 1365 . . 1358 1361 + CviJI RGCY 1359 1359 . . 1354 1359 - Ksp632I* CTCTTC 1349 1352 . . 1354 1360 - SapI GCTCTTC 1349 1352 . . 1383 1387 - Hin4II* CCTTC 1377 1376 . . 1387 1392 + BseRI GAGGAG 1402 1400 . . 1387 1390 - MnlI CCTC 1380 1379 . . 1390 1395 + BseRI GAGGAG 1405 1403 . . 1390 1393 - MnlI CCTC 1383 1382 . . 1395 1400 + NlaIV GGNNCC 1397 1397 . . 1393 1396 - MnlI CCTC 1386 1385 . . 1398 1401 + SetI ASST 1401 1397 . . 1406 1409 + SetI ASST 1409 1405 . . 1406 1409 + CviJI RGCY 1407 1407 . . 1406 1409 + AluI AGCT 1407 1407 . . 1419 1422 + SetI ASST 1422 1418 . . 1420 1423 + MnlI CCTC 1430 1429 . . 1421 1426 - BsiI* CACGAG 1421 1425 . . 1431 1434 + SetI ASST 1434 1430 . . 1447 1452 + Hpy166II GTNNAC 1449 1449 . . 1447 1452 + HindII GTYRAC 1449 1449 . . 1486 1490 + DdeI CTNAG 1486 1489 . . 1491 1502 + BdaI TGANNNNNNTCA 1480 1478 1514 1512 1494 1498 + MboII GAAGA 1506 1505 . . 1497 1501 + TfiI GAWTC 1497 1500 . . 1497 1501 + HinfI GANTC 1497 1500 . . 1491 1502 - BdaI TGANNNNNNTCA 1514 1512 1480 1478 1506 1512 + TspRI CASTGNN 1512 1503 . . 1518 1528 + Hin4I GAYNNNNNVTC 1509 1504 1541 1536 1518 1522 + HgaI GACGC 1527 1532 . . 1522 1525 + MaeI CTAG 1522 1524 . . 1524 1529 + XhoII RGATCY 1524 1528 . . 1525 1528 + DpnI GATC 1526 1526 . . 1525 1528 + BstKTI GATC 1527 1525 . . 1525 1528 + MboI GATC 1524 1528 . . 1528 1531 + MnlI CCTC 1538 1537 . . 1518 1528 - Hin4I GAYNNNNNVTC 1541 1536 1509 1504 1525 1529 - BinI* GGATC 1519 1520 . . 1531 1534 + FatI CATG 1530 1534 . . 1531 1534 + NlaIII CATG 1534 1530 . . 1531 1534 + CviAII CATG 1531 1533 . . 1533 1536 + CviRI* TGCA 1534 1534 . . 1546 1549 + FatI CATG 1545 1549 . . 1546 1549 + NlaIII CATG 1549 1545 . . 1546 1549 + CviAII CATG 1546 1548 . . 1547 1551 + TspDTI ATGAA 1562 1560 . . 1564 1567 + FatI CATG 1563 1567 . . 1564 1567 + NlaIII CATG 1567 1563 . . 1564 1567 + CviAII CATG 1564 1566 . . 1568 1573 + Hpy166II GTNNAC 1570 1570 . . 1568 1571 + Csp6I GTAC 1568 1570 . . 1568 1571 + RsaI GTAC 1569 1569 . . 1585 1588 + TspEI AATT 1584 1588 . . 1590 1593 + SetI ASST 1593 1589 . . 1590 1593 + CviJI RGCY 1591 1591 . . 1590 1593 + AluI AGCT 1591 1591 . . 1591 1595 + BlsI GCNGC 1593 1592 . . 1591 1595 + BisI GCNGC 1592 1593 . . 1591 1595 + TseI GCWGC 1591 1594 . . 1591 1595 - BbvI GCAGC 1578 1582 . . 1592 1597 - EcoP15I CAGCAG 1564 1566 . . 1605 1608 + CviRI* TGCA 1606 1606 . . 1611 1616 + Hpy178III* TCNNGA 1612 1614 . . 1609 1613 - FokI GGATG 1595 1599 . . 1609 1613 - BseGI GGATG 1608 1606 . . 1621 1625 + Hpy188I TCNGA 1623 1622 . . 1627 1630 + FatI CATG 1626 1630 . . 1627 1630 + NlaIII CATG 1630 1626 . . 1627 1630 + CviAII CATG 1627 1629 . . 1630 1634 + TfiI GAWTC 1630 1633 . . 1630 1634 + HinfI GANTC 1630 1633 . . 1633 1637 + Hpy188I TCNGA 1635 1634 . . 1637 1641 + TspDTI ATGAA 1652 1650 . . 1639 1643 + MboII GAAGA 1651 1650 . . 1654 1659 + BsiI* CACGAG 1654 1658 . . 1665 1669 + FokI GGATG 1678 1682 . . 1665 1669 + BseGI GGATG 1671 1669 . . 1663 1666 - MnlI CCTC 1656 1655 . . 1668 1671 + CviRI* TGCA 1669 1669 . . 1666 1670 - SfaNI GCATC 1656 1660 . . 1675 1679 - BccI CCATC 1669 1670 . . 1685 1694 + XmnI GAANNNNTTC 1689 1689 . . 1693 1696 + TaqI TCGA 1693 1695 . . 1689 1694 - BsmI GAATGC 1689 1687 . . 1695 1699 - BccI CCATC 1689 1690 . . 1704 1709 + Cac8I GCNNGC 1706 1706 . . 1706 1711 + HindIII AAGCTT 1706 1710 . . 1707 1710 + SetI ASST 1710 1706 . . 1707 1710 + CviJI RGCY 1708 1708 . . 1707 1710 + AluI AGCT 1708 1708 . . 1713 1717 + Hpy188I TCNGA 1715 1714 . . 1719 1722 + SetI ASST 1722 1718 . . 1729 1733 + PleI GAGTC 1737 1738 . . 1729 1733 + MlyI GAGTC 1738 1738 . . 1729 1733 + HinfI GANTC 1729 1732 . . 1732 1736 + Hpy188I TCNGA 1734 1733 . . 1742 1745 + MseI TTAA 1742 1744 . . 1744 1747 + TspEI AATT 1743 1747 . . 1747 1752 + Hpy178III* TCNNGA 1748 1750 . . 1747 1752 + BetI* WCCGGW 1747 1751 . . 1747 1752 + BspMII* TCCGGA 1747 1751 . . 1748 1751 + HpaII CCGG 1748 1750 . . 1757 1762 + ApoI RAATTY 1757 1761 . . 1758 1761 + TspEI AATT 1757 1761 . . 1765 1768 + MnlI CCTC 1775 1774 . . 1766 1771 + SmlI CTYRAG 1766 1770 . . 1767 1772 + Hpy178III* TCNNGA 1768 1770 . . 1766 1771 - Bce83I* CTTGAG 1751 1749 . . 1787 1791 + Tsp4CI* ACNGT 1789 1788 . . 1790 1793 + Csp6I GTAC 1790 1792 . . 1790 1793 + RsaI GTAC 1791 1791 . . 1793 1798 + PstI CTGCAG 1797 1793 . . 1793 1798 + SfeI* CTRYAG 1793 1797 . . 1794 1797 + CviRI* TGCA 1795 1795 . . 1807 1810 + FatI CATG 1806 1810 . . 1807 1810 + NlaIII CATG 1810 1806 . . 1807 1810 + CviAII CATG 1807 1809 . . 1818 1823 + ApoI RAATTY 1818 1822 . . 1819 1822 + TspEI AATT 1818 1822 . . 1821 1825 - TspDTI ATGAA 1811 1809 . . 1837 1841 + BsrI ACTGG 1842 1840 . . 1855 1859 + BsmAI GTCTC 1860 1864 . . 1857 1862 + Ksp632I* CTCTTC 1863 1866 . . 1858 1862 - MboII GAAGA 1850 1849 . . 1879 1884 + Cac8I GCNNGC 1881 1881 . . 1891 1894 + TaqI TCGA 1891 1893 . . 1896 1901 + ApoI RAATTY 1896 1900 . . 1897 1900 + TspEI AATT 1896 1900 . . 1913 1923 + BsiYI* CCNNNNNNNGG 1919 1916 . . 1931 1936 + EciI GGCGGA 1947 1945 . . 1932 1935 - AciI CCGC 1932 1934 . . 1953 1958 + Hpy178III* TCNNGA 1954 1956 . . 1953 1958 + BspHI TCATGA 1953 1957 . . 1954 1957 + FatI CATG 1953 1957 . . 1954 1957 + NlaIII CATG 1957 1953 . . 1954 1957 + CviAII CATG 1954 1956 . . 1960 1964 + Tsp4CI* ACNGT 1962 1961 . . 1972 1975 + TspEI AATT 1971 1975 . . 1974 1978 - TspDTI ATGAA 1964 1962 . . 1980 1983 + CviRI* TGCA 1981 1981 . . 1984 1987 + MseI TTAA 1984 1986 . . 1994 1997 + AciI CCGC 1994 1996 . . 1994 1999 + BsrBI CCGCTC 1996 1996 . . 1997 2002 + SmlI CTYRAG 1997 2001 . . 1998 2003 + Hpy178III* TCNNGA 1999 2001 . . 1997 2002 - Bce83I* CTTGAG 1982 1980 . . 2008 2011 + TaqI TCGA 2008 2010 . . 2010 2020 + Hin4I GAYNNNNNVTC 2001 1996 2033 2028 2014 2024 + MwoI GCNNNNNNNGC 2020 2017 . . 2014 2018 + BlsI GCNGC 2016 2015 . . 2014 2018 + BisI GCNGC 2015 2016 . . 2010 2014 - BccI CCATC 2004 2005 . . 2014 2018 + BbvI GCAGC 2026 2030 . . 2014 2018 + TseI GCWGC 2014 2017 . . 2016 2019 + SetI ASST 2019 2015 . . 2016 2019 + CviJI RGCY 2017 2017 . . 2016 2019 + AluI AGCT 2017 2017 . . 2017 2027 + MwoI GCNNNNNNNGC 2023 2020 . . 2021 2026 + EcoP15I CAGCAG 2051 2053 . . 2018 2023 - Eco57MI CTGRAG 2003 2001 . . 2018 2023 - GsuI CTGGAG 2003 2001 . . 2023 2027 + BlsI GCNGC 2025 2024 . . 2023 2027 + BisI GCNGC 2024 2025 . . 2023 2027 + BbvI GCAGC 2035 2039 . . 2023 2027 + TseI GCWGC 2023 2026 . . 2025 2028 + SetI ASST 2028 2024 . . 2025 2028 + CviJI RGCY 2026 2026 . . 2025 2028 + AluI AGCT 2026 2026 . . 2027 2032 - Eco57MI CTGRAG 2012 2010 . . 2027 2032 - GsuI CTGGAG 2012 2010 . . 2029 2033 - BsrI ACTGG 2029 2027 . . 2035 2039 + DdeI CTNAG 2035 2038 . . 2042 2045 + SetI ASST 2045 2041 . . 2046 2050 + Tsp45I GTSAC 2045 2050 . . 2046 2050 + MaeIII GTNAC 2045 2050 . . 2052 2057 + Hpy178III* TCNNGA 2053 2055 . . 2053 2059 + SauI* CCTNAGG 2054 2057 . . 2050 2054 - FokI GGATG 2036 2040 . . 2054 2058 + DdeI CTNAG 2054 2057 . . 2050 2054 - BseGI GGATG 2049 2047 . . 2054 2058 - BspCNI CTCAG 2046 2044 . . 2054 2058 - BseMII CTCAG 2045 2043 . . 2056 2059 - MnlI CCTC 2049 2048 . . 2063 2066 + SetI ASST 2066 2062 . . 2064 2069 + NlaIV GGNNCC 2066 2066 . . 2064 2069 + HgiCI* GGYRCC 2064 2068 . . 2070 2073 + SetI ASST 2073 2069 . . 2071 2074 + MnlI CCTC 2081 2080 . . 2074 2079 + TaqII CACCCA 2090 2088 . . 2074 2082 + DraIII CACNNNGTG 2079 2076 . . 2073 2077 - HphI GGTGA 2065 2064 . . 2078 2084 + TspRI CASTGNN 2084 2075 . . 2077 2081 - BsrI ACTGG 2077 2075 . . 2076 2081 - BfiI ACTGGG 2071 2070 . . 2083 2087 + Hin4II* CCTTC 2093 2092 . . 2088 2091 + CviRI* TGCA 2089 2089 . . 2089 2099 + MwoI GCNNNNNNNGC 2095 2092 . . 2089 2099 + BstAPI GCANNNNNTGC 2095 2092 . . 2087 2092 - BsgI GTGCAG 2072 2070 . . 2093 2096 + MnlI CCTC 2103 2102 . . 2097 2100 + CviRI* TGCA 2098 2098 . . 2100 2103 + SetI ASST 2103 2099 . . 2101 2104 + MnlI CCTC 2111 2110 . . 2096 2101 - BsgI GTGCAG 2081 2079 . . 2102 2107 - Eco57MI CTGRAG 2087 2085 . . 2107 2110 + Csp6I GTAC 2107 2109 . . 2102 2107 - GsuI CTGGAG 2087 2085 . . 2107 2110 + RsaI GTAC 2108 2108 . . 2104 2108 - BsrI ACTGG 2104 2102 . . 2111 2114 + MnlI CCTC 2121 2120 . . 2115 2118 + CviRI* TGCA 2116 2116 . . 2118 2121 + SetI ASST 2121 2117 . . 2119 2123 + Hin4II* CCTTC 2129 2128 . . 2114 2119 - BsgI GTGCAG 2099 2097 . . 2120 2125 - Eco57MI CTGRAG 2105 2103 . . 2125 2128 + Csp6I GTAC 2125 2127 . . 2125 2128 + RsaI GTAC 2126 2126 . . 2120 2125 - Eco57I CTGAAG 2105 2103 . . 2129 2132 + MnlI CCTC 2139 2138 . . 2133 2136 + CviRI* TGCA 2134 2134 . . 2136 2139 + SetI ASST 2139 2135 . . 2137 2140 + MnlI CCTC 2147 2146 . . 2132 2137 - BsgI GTGCAG 2117 2115 . . 2138 2143 - Eco57MI CTGRAG 2123 2121 . . 2143 2146 + Csp6I GTAC 2143 2145 . . 2138 2143 - GsuI CTGGAG 2123 2121 . . 2143 2146 + RsaI GTAC 2144 2144 . . 2140 2144 - BsrI ACTGG 2140 2138 . . 2145 2148 + SetI ASST 2148 2144 . . 2146 2149 + MnlI CCTC 2156 2155 . . 2147 2152 - Eco57MI CTGRAG 2132 2130 . . 2152 2162 + MwoI GCNNNNNNNGC 2158 2155 . . 2147 2152 - GsuI CTGGAG 2132 2130 . . 2154 2157 + SetI ASST 2157 2153 . . 2155 2158 + MnlI CCTC 2165 2164 . . 2156 2161 - Eco57MI CTGRAG 2141 2139 . . 2161 2171 + MwoI GCNNNNNNNGC 2167 2164 . . 2161 2171 + BstAPI GCANNNNNTGC 2167 2164 . . 2156 2161 - GsuI CTGGAG 2141 2139 . . 2169 2172 + CviRI* TGCA 2170 2170 . . 2172 2175 + SetI ASST 2175 2171 . . 2173 2177 + Hin4II* CCTTC 2183 2182 . . 2168 2173 - BsgI GTGCAG 2153 2151 . . 2174 2179 - Eco57MI CTGRAG 2159 2157 . . 2179 2182 + Csp6I GTAC 2179 2181 . . 2179 2182 + RsaI GTAC 2180 2180 . . 2174 2179 - Eco57I CTGAAG 2159 2157 . . 2188 2191 + Csp6I GTAC 2188 2190 . . 2188 2191 + RsaI GTAC 2189 2189 . . 2185 2189 - BsrI ACTGG 2185 2183 . . 2184 2189 - BfiI ACTGGG 2179 2178 . . 2197 2200 + Csp6I GTAC 2197 2199 . . 2197 2200 + RsaI GTAC 2198 2198 . . 2194 2198 - BsrI ACTGG 2194 2192 . . 2193 2198 - BfiI ACTGGG 2188 2187 . . 2199 2202 + SetI ASST 2202 2198 . . 2200 2203 + MnlI CCTC 2210 2209 . . 2201 2206 - Eco57MI CTGRAG 2186 2184 . . 2201 2206 - GsuI CTGGAG 2186 2184 . . 2203 2207 - BsrI ACTGG 2203 2201 . . 2209 2213 - MboII GAAGA 2201 2200 . . 2210 2215 - Eco57MI CTGRAG 2195 2193 . . 2215 2225 + MwoI GCNNNNNNNGC 2221 2218 . . 2210 2215 - Eco57I CTGAAG 2195 2193 . . 2217 2220 + SetI ASST 2220 2216 . . 2218 2221 + MnlI CCTC 2228 2227 . . 2219 2224 - Eco57MI CTGRAG 2204 2202 . . 2224 2234 + MwoI GCNNNNNNNGC 2230 2227 . . 2219 2224 - GsuI CTGGAG 2204 2202 . . 2229 2233 + DdeI CTNAG 2229 2232 . . 2229 2233 + BspCNI CTCAG 2242 2240 . . 2229 2233 + BseMII CTCAG 2243 2241 . . 2235 2238 + SetI ASST 2238 2234 . . 2236 2240 + Hin4II* CCTTC 2246 2245 . . 2244 2247 + SetI ASST 2247 2243 . . 2245 2248 + MnlI CCTC 2255 2254 . . 2246 2251 - Eco57MI CTGRAG 2231 2229 . . 2251 2254 + Csp6I GTAC 2251 2253 . . 2246 2251 - GsuI CTGGAG 2231 2229 . . 2251 2254 + RsaI GTAC 2252 2252 . . 2248 2252 - BsrI ACTGG 2248 2246 . . 2253 2256 + SetI ASST 2256 2252 . . 2254 2257 + MnlI CCTC 2264 2263 . . 2256 2261 + MmeI TCCRAC 2281 2279 . . 2262 2265 + SetI ASST 2265 2261 . . 2263 2266 + MnlI CCTC 2273 2272 . . 2264 2269 - Eco57MI CTGRAG 2249 2247 . . 2269 2279 + MwoI GCNNNNNNNGC 2275 2272 . . 2264 2269 - GsuI CTGGAG 2249 2247 . . 2271 2274 + SetI ASST 2274 2270 . . 2272 2275 + MnlI CCTC 2282 2281 . . 2273 2278 - Eco57MI CTGRAG 2258 2256 . . 2278 2288 + MwoI GCNNNNNNNGC 2284 2281 . . 2273 2278 - GsuI CTGGAG 2258 2256 . . 2280 2283 + SetI ASST 2283 2279 . . 2281 2284 + MnlI CCTC 2291 2290 . . 2286 2291 + HaeII RGCGCY 2290 2286 . . 2287 2290 + Hin6I GCGC 2287 2289 . . 2287 2290 + GlaI GCGC 2288 2288 . . 2282 2287 - Eco57MI CTGRAG 2267 2265 . . 2287 2297 + MwoI GCNNNNNNNGC 2293 2290 . . 2282 2287 - GsuI CTGGAG 2267 2265 . . 2287 2290 + HhaI GCGC 2289 2287 . . 2289 2299 + MwoI GCNNNNNNNGC 2295 2292 . . 2290 2293 + MnlI CCTC 2300 2299 . . 2296 2299 + Hin6I GCGC 2296 2298 . . 2296 2299 + GlaI GCGC 2297 2297 . . 2296 2299 + HhaI GCGC 2298 2296 . . 2298 2302 + BlsI GCNGC 2300 2299 . . 2298 2302 + BisI GCNGC 2299 2300 . . 2298 2302 + TseI GCWGC 2298 2301 . . 2298 2302 - BbvI GCAGC 2285 2289 . . 2312 2316 + TspDTI ATGAA 2327 2325 . . 2320 2324 + MboII GAAGA 2332 2331 . . 2325 2330 + BspLU11I* ACATGT 2325 2329 . . 2325 2330 + NspI RCATGY 2329 2325 . . 2325 2330 + AflIII ACRYGT 2325 2329 . . 2326 2329 + FatI CATG 2325 2329 . . 2326 2329 + NlaIII CATG 2329 2325 . . 2326 2329 + CviAII CATG 2326 2328 . . 2346 2350 + Hin4II* CCTTC 2356 2355 . . 2352 2355 + SetI ASST 2355 2351 . . 2356 2361 + BciVI GTATCC 2367 2366 . . 2359 2363 + Hpy188I TCNGA 2361 2360 . . 2360 2365 + SecI* CCNNGG 2360 2364 . . 2362 2365 - MnlI CCTC 2355 2354 . . 2371 2375 - FokI GGATG 2357 2361 . . 2371 2375 - BseGI GGATG 2370 2368 . . 2392 2395 + AciI CCGC 2392 2394 . . 2391 2396 - EciI GGCGGA 2381 2379 . . 2402 2407 + BsaAI YACGTR 2404 2404 . . 2402 2407 + PmaCI CACGTG 2404 2404 . . 2403 2406 + TaiI ACGT 2406 2402 . . 2403 2406 + SetI ASST 2406 2402 . . 2403 2406 + MaeII ACGT 2403 2405 . . 2405 2410 + Hpy166II GTNNAC 2407 2407 . . 2405 2410 + SduI GDGCHC 2409 2405 . . 2405 2410 + BseSI GKGCMC 2409 2405 . . 2405 2410 + ApaLI GTGCAC 2405 2409 . . 2405 2410 + HgiAI* GWGCWC 2409 2405 . . 2406 2409 + CviRI* TGCA 2407 2407 . . 2416 2419 + Csp6I GTAC 2416 2418 . . 2416 2419 + RsaI GTAC 2417 2417 . . 2413 2417 - BsrI ACTGG 2413 2411 . . 2421 2426 + Cfr10I RCCGGY 2421 2425 . . 2422 2425 + HpaII CCGG 2422 2424 . . 2424 2427 + CviJI RGCY 2425 2425 . . 2434 2439 + ApoI RAATTY 2434 2438 . . 2435 2438 + TspEI AATT 2434 2438 . . 2440 2444 + HinfI GANTC 2440 2443 . . 2443 2447 + Hpy188I TCNGA 2445 2444 . . 2440 2444 - PleI GAGTC 2434 2435 . . 2440 2444 - MlyI GAGTC 2434 2434 . . 2454 2457 + CviJI RGCY 2455 2455 . . 2472 2475 + SetI ASST 2475 2471 . . 2472 2475 + CviJI RGCY 2473 2473 . . 2472 2475 + AluI AGCT 2473 2473 . . 2476 2480 + Hin4II* CCTTC 2486 2485 . . 2482 2485 + MnlI CCTC 2492 2491 . . 2480 2485 - BseRI GAGGAG 2471 2469 . . 2491 2500 + MslI CAYNNNNRTG 2495 2495 . . 2500 2504 + Tsp45I GTSAC 2499 2504 . . 2500 2504 + MaeIII GTNAC 2499 2504 . . 2503 2507 + BceAI ACGGC 2519 2521 . . 2505 2508 + CviJI RGCY 2506 2506 . . 2505 2508 + HaeIII GGCC 2506 2506 . . 2507 2510 + MnlI CCTC 2517 2516 . . 2514 2517 + SetI ASST 2517 2513 . . 2515 2519 + Hin4II* CCTTC 2525 2524 . . 2528 2531 + Csp6I GTAC 2528 2530 . . 2528 2531 + RsaI GTAC 2529 2529 . . 2538 2541 + TaiI ACGT 2541 2537 . . 2538 2541 + SetI ASST 2541 2537 . . 2538 2541 + MaeII ACGT 2538 2540 . . 2538 2543 + AflIII ACRYGT 2538 2542 . . 2548 2551 + SetI ASST 2551 2547 . . 2549 2552 + MnlI CCTC 2559 2558 . . 2550 2554 + DdeI CTNAG 2550 2553 . . 2550 2554 + BspCNI CTCAG 2563 2561 . . 2550 2554 + BseMII CTCAG 2564 2562 . . 2553 2558 + TatI WGTACW 2553 2557 . . 2553 2558 + ScaI AGTACT 2555 2555 . . 2554 2557 + Csp6I GTAC 2554 2556 . . 2554 2557 + RsaI GTAC 2555 2555 . . 2575 2579 + TfiI GAWTC 2575 2578 . . 2575 2579 + HinfI GANTC 2575 2578 . . 2578 2583 + Hpy178III* TCNNGA 2579 2581 . . 2590 2593 + AciI CCGC 2590 2592 . . 2592 2602 + MwoI GCNNNNNNNGC 2598 2595 . . 2596 2599 + FatI CATG 2595 2599 . . 2596 2599 + NlaIII CATG 2599 2595 . . 2596 2599 + CviAII CATG 2596 2598 . . 2602 2606 + Hin4II* CCTTC 2612 2611 . . 2604 2608 - TspDTI ATGAA 2594 2592 . . 2610 2620 + Hin4I GAYNNNNNVTC 2601 2596 2633 2628 2610 2620 + Eam1105I GACNNNNNGTC 2615 2614 . . 2613 2617 + TfiI GAWTC 2613 2616 . . 2613 2617 + HinfI GANTC 2613 2616 . . 2610 2620 - Hin4I GAYNNNNNVTC 2633 2628 2601 2596 2626 2630 + TfiI GAWTC 2626 2629 . . 2626 2630 + HinfI GANTC 2626 2629 . . 2628 2632 - TspDTI ATGAA 2618 2616 . . 2635 2638 + FatI CATG 2634 2638 . . 2635 2638 + NlaIII CATG 2638 2634 . . 2635 2638 + CviAII CATG 2635 2637 . . 2638 2642 + PleI GAGTC 2646 2647 . . 2638 2642 + MlyI GAGTC 2647 2647 . . 2638 2642 + HinfI GANTC 2638 2641 . . 2641 2645 + Hpy188I TCNGA 2643 2642 . . 2657 2660 + SetI ASST 2660 2656 . . 2655 2659 - Hin4II* CCTTC 2649 2648 . . 2661 2664 + CviJI RGCY 2662 2662 . . 2661 2664 + HaeIII GGCC 2662 2662 . . 2663 2666 + MnlI CCTC 2673 2672 . . 2674 2677 + SetI ASST 2677 2673 . . 2672 2676 - Hin4II* CCTTC 2666 2665 . . 2676 2681 + Hpy166II GTNNAC 2678 2678 . . 2676 2681 + HindII GTYRAC 2678 2678 . . 2681 2685 + Hpy99I CGWCG 2685 2680 . . 2682 2686 + HgaI GACGC 2691 2696 . . 2686 2689 + FatI CATG 2685 2689 . . 2686 2689 + NlaIII CATG 2689 2685 . . 2686 2689 + CviAII CATG 2686 2688 . . 2704 2709 + MmeI TCCRAC 2729 2727 . . 2711 2714 + TaiI ACGT 2714 2710 . . 2711 2714 + SetI ASST 2714 2710 . . 2711 2714 + MaeII ACGT 2711 2713 . . 2714 2719 + Hpy178III* TCNNGA 2715 2717 . . 2726 2729 + MseI TTAA 2726 2728 . . 2730 2733 + CviRI* TGCA 2731 2731 . . 2741 2745 - BccI CCATC 2735 2736 . . 2749 2754 + VspI ATTAAT 2750 2752 . . 2750 2753 + MseI TTAA 2750 2752 . . 2759 2762 + Hin6I GCGC 2759 2761 . . 2759 2762 + GlaI GCGC 2760 2760 . . 2759 2762 + HhaI GCGC 2761 2759 . . 2761 2771 + MwoI GCNNNNNNNGC 2767 2764 . . 2761 2771 + BstAPI GCANNNNNTGC 2767 2764 . . 2763 2766 + SetI ASST 2766 2762 . . 2764 2767 + MnlI CCTC 2774 2773 . . 2781 2785 + Hpy188I TCNGA 2783 2782 . . 2782 2787 + Eco57MI CTGRAG 2803 2801 . . 2782 2787 + Eco57I CTGAAG 2803 2801 . . 2785 2795 + FalI AAGNNNNNCTT 2776 2771 2808 2803 2785 2795 - FalI AAGNNNNNCTT 2808 2803 2776 2771 2799 2808 + BsaBI GATNNNNATC 2803 2803 . . 2799 2803 - BccI CCATC 2793 2794 . . 2805 2815 + Hin4I GAYNNNNNVTC 2796 2791 2828 2823 2805 2808 + DpnI GATC 2806 2806 . . 2805 2808 + BstKTI GATC 2807 2805 . . 2805 2808 + MboI GATC 2804 2808 . . 2806 2811 + ClaI ATCGAT 2807 2809 . . 2807 2810 + TaqI TCGA 2807 2809 . . 2811 2816 + BclI TGATCA 2811 2815 . . 2812 2815 + DpnI GATC 2813 2813 . . 2812 2815 + BstKTI GATC 2814 2812 . . 2812 2815 + MboI GATC 2811 2815 . . 2805 2815 - Hin4I GAYNNNNNVTC 2828 2823 2796 2791 2818 2822 - TspDTI ATGAA 2808 2806 . . 2845 2850 - TaqII CACCCA 2835 2833 . . 2890 2894 + DdeI CTNAG 2890 2893 . . 2894 2898 + TfiI GAWTC 2894 2897 . . 2894 2898 + HinfI GANTC 2894 2897 . . 2905 2908 + SetI ASST 2908 2904 . . 2909 2912 + MseI TTAA 2909 2911 . . 2914 2919 + TatI WGTACW 2914 2918 . . 2915 2918 + Csp6I GTAC 2915 2917 . . 2915 2918 + RsaI GTAC 2916 2916 . . 2920 2923 + CviJI RGCY 2921 2921 . . 2932 2936 + Tsp4CI* ACNGT 2934 2933 . . 2935 2939 + MaeIII GTNAC 2934 2939 . . 2940 2944 + HinfI GANTC 2940 2943 . . 2942 2947 + Ksp632I* CTCTTC 2948 2951 . . 2940 2944 - PleI GAGTC 2934 2935 . . 2940 2944 - MlyI GAGTC 2934 2934 . . 2946 2951 + Hpy178III* TCNNGA 2947 2949 . . 2943 2947 - MboII GAAGA 2935 2934 . . 2935 2947 - BarI GAAGNNNNNNTAC 2959 2954 2927 2922 2950 2959 + XmnI GAANNNNTTC 2954 2954 . . 2959 2963 + BccI CCATC 2967 2968 . . 2960 2965 - BtgZI GCGATG 2945 2949 . . 2969 2972 + TaqI TCGA 2969 2971 . . 2965 2969 - BsrI ACTGG 2965 2963 . . 2984 2989 + BsaAI YACGTR 2986 2986 . . 2985 2988 + TaiI ACGT 2988 2984 . . 2985 2988 + SetI ASST 2988 2984 . . 2985 2988 + MaeII ACGT 2985 2987 . . 2991 2996 + Hpy178III* TCNNGA 2992 2994 . . 2996 3000 + TspDTI ATGAA 3011 3009 . . 2997 3008 + BdaI TGANNNNNNTCA 2986 2984 3020 3018 2997 3008 - BdaI TGANNNNNNTCA 3020 3018 2986 2984 3010 3013 + SetI ASST 3013 3009 . . 3014 3017 + MseI TTAA 3014 3016 . . 3042 3046 - TspDTI ATGAA 3032 3030 . . 3051 3056 + ApoI RAATTY 3051 3055 . . 3052 3055 + TspEI AATT 3051 3055 . . 3066 3069 + CviJI RGCY 3067 3067 . . 3068 3072 + DdeI CTNAG 3068 3071 . . 3072 3077 + ApoI RAATTY 3072 3076 . . 3073 3076 + TspEI AATT 3072 3076 . . 3081 3085 + Tsp45I GTSAC 3080 3085 . . 3081 3085 + MaeIII GTNAC 3080 3085 . . 3087 3090 + TspEI AATT 3086 3090 . . 3091 3096 + BciVI GTATCC 3102 3101 . . 3094 3098 + Hpy188I TCNGA 3096 3095 . . 3097 3102 + EcoRI GAATTC 3097 3101 . . 3097 3102 + ApoI RAATTY 3097 3101 . . 3098 3101 + TspEI AATT 3097 3101 . . 3135 3140 + AclI AACGTT 3136 3138 . . 3136 3139 + TaiI ACGT 3139 3135 . . 3136 3139 + SetI ASST 3139 3135 . . 3136 3139 + MaeII ACGT 3136 3138 . . 3143 3146 + Hin6I GCGC 3143 3145 . . 3143 3146 + GlaI GCGC 3144 3144 . . 3143 3146 + HhaI GCGC 3145 3143 . . 3159 3164 + PsiI TTATAA 3161 3161 . . 3165 3168 + SetI ASST 3168 3164 . . 3166 3176 + EcoNI CCTNNNNNAGG 3170 3171 . . 3166 3176 + BsiYI* CCNNNNNNNGG 3172 3169 . . 3167 3172 + Eco57MI CTGRAG 3188 3186 . . 3167 3172 + Eco57I CTGAAG 3188 3186 . . 3169 3173 + MboII GAAGA 3181 3180 . . 3176 3180 + TfiI GAWTC 3176 3179 . . 3172 3176 - Hin4II* CCTTC 3166 3165 . . 3176 3180 + HinfI GANTC 3176 3179 . . 3179 3182 + TaqI TCGA 3179 3181 . . 3183 3187 + Tsp4CI* ACNGT 3185 3184 . . 3190 3194 + TspDTI ATGAA 3205 3203 . . 3192 3196 + MboII GAAGA 3204 3203 . . 3196 3199 + SetI ASST 3199 3195 . . 3196 3199 + CviJI RGCY 3197 3197 . . 3196 3199 + AluI AGCT 3197 3197 . . 3192 3197 - Ksp632I* CTCTTC 3187 3190 . . 3192 3198 - SapI GCTCTTC 3187 3190 . . 3204 3208 + MboII GAAGA 3216 3215 . . 3209 3212 + MseI TTAA 3209 3211 . . 3213 3219 + SexAI ACCWGGT 3213 3218 . . 3214 3218 + BssKI CCNGG 3213 3218 . . 3214 3218 + BseBI CCWGG 3215 3216 . . 3214 3218 + ScrFI CCNGG 3215 3216 . . 3214 3218 + EcoRII CCWGG 3213 3218 . . 3216 3219 + SetI ASST 3219 3215 . . 3217 3221 + HphI GGTGA 3229 3228 . . 3218 3222 + Tsp45I GTSAC 3217 3222 . . 3218 3222 + MaeIII GTNAC 3217 3222 . . 3231 3234 + TspEI AATT 3230 3234 . . 3240 3243 + CviJI RGCY 3241 3241 . . 3240 3243 + HaeIII GGCC 3241 3241 . . 3244 3249 + ApoI RAATTY 3244 3248 . . 3245 3248 + TspEI AATT 3244 3248 . . 3252 3255 + SetI ASST 3255 3251 . . 3252 3255 + CviJI RGCY 3253 3253 . . 3252 3255 + AluI AGCT 3253 3253 . . 3264 3267 + DpnI GATC 3265 3265 . . 3264 3267 + BstKTI GATC 3266 3264 . . 3264 3267 + MboI GATC 3263 3267 . . 3266 3270 + Hpy188I TCNGA 3268 3267 . . 3279 3282 + TspEI AATT 3278 3282 . . 3286 3291 + AclI AACGTT 3287 3289 . . 3287 3290 + TaiI ACGT 3290 3286 . . 3287 3290 + SetI ASST 3290 3286 . . 3287 3290 + MaeII ACGT 3287 3289 . . 3289 3294 - MmeI TCCRAC 3270 3268 . . 3298 3302 + HinfI GANTC 3298 3301 . . 3298 3302 - PleI GAGTC 3292 3293 . . 3298 3302 - MlyI GAGTC 3292 3292 . . 3302 3305 + AciI CCGC 3302 3304 . . 3304 3308 + TauI GCSGC 3307 3304 . . 3304 3308 + BlsI GCNGC 3306 3305 . . 3304 3308 + BisI GCNGC 3305 3306 . . 3305 3308 + AciI CCGC 3305 3307 . . 3305 3310 + BsrBI CCGCTC 3307 3307 . . 3301 3306 - EciI GGCGGA 3291 3289 . . 3309 3313 - TspGWI ACGGA 3299 3297 . . 3316 3319 + TaqI TCGA 3316 3318 . . 3325 3329 + MaeIII GTNAC 3324 3329 . . 3365 3368 + TaqI TCGA 3365 3367 . . 3372 3377 + Hpy178III* TCNNGA 3373 3375 . . 3373 3378 + BsiI* CACGAG 3373 3377 . . 3376 3379 - MnlI CCTC 3369 3368 . . 3397 3400 + SetI ASST 3400 3396 . . 3397 3400 + CviJI RGCY 3398 3398 . . 3397 3400 + AluI AGCT 3398 3398 . . 3404 3407 + SetI ASST 3407 3403 . . 3404 3407 + CviJI RGCY 3405 3405 . . 3404 3407 + AluI AGCT 3405 3405 . . 3405 3411 + EspI* GCTNAGC 3406 3409 . . 3406 3410 + DdeI CTNAG 3406 3409 . . 3409 3412 + CviJI RGCY 3410 3410 . . 3411 3425 + XcmI CCANNNNNNNNNTGG 3418 3417 . . 3412 3417 + NdeI CATATG 3413 3415 . . 3415 3419 + TspDTI ATGAA 3430 3428 . . 3430 3433 + MseI TTAA 3430 3432 . . 3434 3437 + SetI ASST 3437 3433 . . 3440 3444 + Hpy188I TCNGA 3442 3441 . . 3444 3447 + TspEI AATT 3443 3447 . . 3455 3458 - MnlI CCTC 3448 3447 . . 3460 3465 + SnaBI TACGTA 3462 3462 . . 3460 3465 + BsaAI YACGTR 3462 3462 . . 3461 3464 + TaiI ACGT 3464 3460 . . 3461 3464 + SetI ASST 3464 3460 . . 3461 3464 + MaeII ACGT 3461 3463 . . 3457 3462 - BciVI GTATCC 3451 3450 . . 3465 3468 + SetI ASST 3468 3464 . . #--------------------------------------- # # # AciI: # [CCGC] # 3 21 21 62 105 169 # 198 256 356 398 488 685 # 712 # # AclI: # [AACGTT] # 151 187 3136 # # AcyI: # [GRCGYC] # 116 3358 # # AflIII: # [ACRYGT] # 213 936 2325 # # AgeI: # [ACCGGT] # 241 3233 # # AhaIII*: # [TTTAAA] # 71 3403 # # AjuI: # [GAANNNNNNNTTGG] # 32 113 3329 # # AluI: # [AGCT] # 7 9 56 69 117 145 # 184 309 447 546 724 861 # # ApaLI: # [GTGCAC] # 707 1069 1698 # # ApoI: # [RAATTY] # 21 25 61 72 78 147 # 230 338 538 548 617 799 # # AsuI*: # [GGNCC] # 49 83 288 824 2230 # # AvaII: # [GGWCC] # 132 288 3054 # # BaeI: # [ACNNNNGTAYC] # 33 33 117 242 3049 # # BarI: # [GAAGNNNNNNTAC] # 32 515 2927 # # BbvCI: # [CCTCAGC] # 1154 2320 # # BbvI: # [GCAGC] # 9 250 448 768 810 1189 # # BbvII*: # [GAAGAC] # 119 1071 2284 # # BccI: # [CCATC] # 20 58 174 313 315 507 # 731 1356 # # Bce83I*: # [CTTGAG] # 231 864 887 1492 # # BceAI: # [ACGGC] # 955 1191 1328 # # BciVI: # [GTATCC] # 23 349 735 2367 # # BclI: # [TGATCA] # 663 2811 # # BdaI: # [TGANNNNNNTCA] # 0 0 0 0 0 0 # 34 34 34 67 454 1379 # 1472 # # BetI*: # [WCCGGW] # 241 1506 1727 # # BfiI: # [ACTGGG] # 9 108 1286 2071 # # BinI*: # [GGATC] # 210 1309 1955 # # BisI: # [GCNGC] # 9 21 21 169 275 345 # 356 423 423 426 1006 # # BlsI: # [GCNGC] # 9 21 21 168 275 345 # 357 423 423 426 1006 # # BmgT120I: # [GGNCC] # 49 83 289 824 2229 # # Bpu10I: # [CCTNAGC] # 1154 2320 # # BsaAI: # [YACGTR] # 12 476 582 2404 # # BsaBI: # [GATNNNNATC] # 671 2803 # # BsaXI: # [ACNNNNNCTCC] # 30 1133 2311 # # BseBI: # [CCWGG] # 142 259 475 2598 # # BseGI: # [GGATG] # 63 321 378 1104 1608 # # BseMII: # [CTCAG] # 49 180 193 198 237 321 # 486 900 910 # # BseRI: # [GAGGAG] # 3 25 248 1003 1066 1129 # # BseSI: # [GKGCMC] # 20 691 1065 1698 # # BsgI: # [GTGCAG] # 9 18 18 36 1321 2072 # # BsiI*: # [CACGAG] # 101 233 1421 1719 # # BsiYI*: # [CCNNNNNNNGG] # 241 302 1253 1678 # # BslFI: # [GGGAC] # 50 595 2829 # # BsmAI: # [GTCTC] # 1614 1860 # # BsmI: # [GAATGC] # 1689 1785 # # BspCNI: # [CTCAG] # 48 180 195 196 237 321 # 486 900 911 # # BspHI: # [TCATGA] # 1521 1953 # # BspLU11I*: # [ACATGT] # 1149 2325 # # BspMII*: # [TCCGGA] # 1727 1747 # # BsrBI: # [CCGCTC] # 167 1311 1996 # # BsrI: # [ACTGG] # 6 9 9 21 21 27 # 36 45 45 48 165 187 # 340 509 552 1454 # # BssKI: # [CCNGG] # 140 194 261 281 2598 # # BstAPI: # [GCANNNNNTGC] # 72 600 707 2095 # # BstKTI: # [GATC] # 7 87 118 208 452 1280 # 1322 # # BtgZI: # [GCGATG] # 529 2945 # # Cac8I: # [GCNNGC] # 175 489 1217 1593 # # CauII*: # [CCSGG] # 336 3138 # # Cfr10I: # [RCCGGY] # 241 1053 2180 # # CfrI: # [YGGCCR] # 1176 2298 # # ClaI: # [ATCGAT] # 667 2807 # # Csp6I: # [GTAC] # 9 9 18 18 26 36 # 54 112 126 134 165 222 # 317 361 479 559 829 # # CviAII: # [CATG] # 15 18 38 39 51 63 # 147 180 270 306 351 372 # 409 427 788 # # CviJI: # [RGCY] # 5 5 7 9 12 16 # 18 21 21 21 30 30 # 32 33 33 34 39 42 # 44 47 47 48 60 64 # 68 90 117 130 145 146 # 156 184 189 259 275 289 # 309 399 # # CviRI*: # [TGCA] # 9 18 18 36 63 72 # 108 117 126 186 237 324 # 708 709 743 # # DdeI: # [CTNAG] # 19 35 68 117 120 175 # 178 180 216 321 332 338 # 340 486 549 # # DpnI: # [GATC] # 7 86 118 209 452 1280 # 1322 # # DraII: # [RGGNCCY] # 288 3186 # # DraIII: # [CACNNNGTG] # 1395 2079 # # DsaI*: # [CCRYGG] # 464 3010 # # Eam1105I: # [GACNNNNNGTC] # 859 2615 # # EciI: # [GGCGGA] # 183 434 910 1947 # # Eco57I: # [CTGAAG] # 36 54 227 286 385 608 # 711 1167 # # Eco57MI: # [CTGRAG] # 9 9 9 9 9 9 # 9 18 18 18 18 27 # 27 75 227 286 385 414 # 536 651 711 # # EcoNI: # [CCTNNNNNAGG] # 304 3170 # # EcoP15I: # [CAGCAG] # 434 487 1130 1423 # # EcoRI: # [GAATTC] # 377 620 2477 # # EcoRII: # [CCWGG] # 140 261 475 2598 # # EspI*: # [GCTNAGC] # 68 857 2549 # # FalI: # [AAGNNNNNCTT] # 0 0 0 0 32 32 # 475 666 2269 # # FatI: # [CATG] # 15 18 38 39 51 63 # 147 180 270 306 351 372 # 409 426 789 # # FnuDII*: # [CGCG] # 1037 2437 # # FokI: # [GGATG] # 83 321 358 1117 1595 # # GlaI: # [GCGC] # 9 330 384 463 1007 1281 # # GsuI: # [CTGGAG] # 9 9 9 9 9 18 # 18 27 36 45 75 651 # 1207 1352 # # HaeII: # [RGCGCY] # 1007 1184 1283 # # HaeIII: # [GGCC] # 68 156 233 339 579 839 # 1260 # # HgaI: # [GACGC] # 124 783 1164 1403 # # HgiAI*: # [GWGCWC] # 269 319 711 1065 1110 # # HgiCI*: # [GGYRCC] # 1410 2064 # # HgiJII*: # [GRGCYC] # 302 603 2569 # # HhaI: # [GCGC] # 9 329 384 463 1007 1282 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 0 0 # 32 32 32 32 163 460 # 568 646 1509 # # Hin4II*: # [CCTTC] # 17 30 36 37 39 51 # 54 63 87 110 130 273 # 284 292 308 447 500 716 # # Hin6I: # [GCGC] # 9 331 384 463 1007 1280 # # HindII: # [GTYRAC] # 558 796 891 1229 # # HindIII: # [AAGCTT] # 1706 1768 # # HinfI: # [GANTC] # 12 13 38 46 53 81 # 99 119 122 133 135 145 # 176 208 236 243 256 648 # 711 # # HpaI: # [GTTAAC] # 891 2583 # # HpaII: # [CCGG] # 29 64 242 674 1052 1413 # # HphI: # [GGTGA] # 145 245 1164 1920 # # Hpy166II: # [GTNNAC] # 7 121 182 200 271 358 # 702 796 837 # # Hpy178III*: # [TCNNGA] # 20 45 45 45 54 88 # 89 101 136 136 138 152 # 186 232 281 331 381 488 # 526 # # Hpy188I: # [TCNGA] # 12 19 29 32 54 80 # 84 140 153 172 174 198 # 247 270 313 627 870 # # Hpy99I: # [CGWCG] # 160 789 2525 # # Ksp632I*: # [CTCTTC] # 15 123 239 287 514 1085 # 1211 # # MaeI: # [CTAG] # 239 1283 1952 # # MaeII: # [ACGT] # 13 55 100 103 135 151 # 151 173 174 242 274 379 # 1524 # # MaeIII: # [GTNAC] # 53 99 107 137 146 150 # 435 454 874 1019 # # MboI: # [GATC] # 7 84 118 211 452 1280 # 1322 # # MboII: # [GAAGA] # 3 6 9 9 12 23 # 33 41 84 107 123 124 # 131 140 145 165 199 246 # 255 258 351 407 603 # # MfeI: # [CAATTG] # 581 2893 # # MlyI: # [GAGTC] # 182 213 287 358 612 696 # 1126 # # MmeI: # [TCCRAC] # 192 204 448 457 541 668 # 964 # # MnlI: # [CCTC] # 3 3 7 8 8 9 # 9 9 9 9 9 9 # 10 12 15 17 18 18 # 18 22 25 26 27 32 # 36 42 44 45 54 55 # 63 66 79 101 105 108 # 114 118 119 137 168 274 # 595 819 # # MseI: # [TTAA] # 24 28 44 50 70 105 # 111 159 195 198 221 242 # 420 432 433 742 # # MslI: # [CAYNNNNRTG] # 568 979 1927 # # MwoI: # [GCNNNNNNNGC] # 2 3 9 9 9 9 # 9 45 54 63 72 169 # 303 403 707 720 888 # # NcoI: # [CCATGG] # 464 3010 # # NdeI: # [CATATG] # 61 3413 # # NlaIII: # [CATG] # 15 18 38 39 51 63 # 147 180 270 306 351 372 # 409 430 785 # # NlaIV: # [GGNNCC] # 246 408 669 743 1408 # # NspI: # [RCATGY] # 1145 2329 # # PleI: # [GAGTC] # 182 212 288 358 612 697 # 1125 # # PmaCI: # [CACGTG] # 1070 2404 # # PpuMI: # [RGGWCCY] # 288 3186 # # PsiI: # [TTATAA] # 313 3161 # # PstI: # [CTGCAG] # 1677 1797 # # RsaI: # [GTAC] # 9 9 18 18 26 36 # 54 112 127 134 165 222 # 317 361 479 558 829 # # SapI: # [GCTCTTC] # 287 1349 1838 # # SauI*: # [CCTNAGG] # 1420 2054 # # ScaI: # [AGTACT] # 919 2555 # # ScrFI: # [CCNGG] # 142 194 259 281 2598 # # SduI: # [GDGCHC] # 20 75 194 282 319 409 # 1065 1110 # # SecI*: # [CCNNGG] # 151 464 1114 1745 # # SetI: # [ASST] # 4 6 7 7 8 9 # 9 9 9 9 9 9 # 9 10 12 12 13 17 # 17 18 18 18 18 18 # 19 20 21 24 25 27 # 27 29 29 30 30 31 # 32 35 36 37 42 51 # 52 55 69 70 72 75 # 76 76 77 80 100 109 # 110 117 126 129 140 142 # 147 148 159 228 297 # # SexAI: # [ACCWGGT] # 261 3213 # # SfaNI: # [GCATC] # 582 1074 1818 # # SfeI*: # [CTRYAG] # 21 21 359 1392 1681 # # SmlI: # [CTYRAG] # 231 843 923 1477 # # SnaBI: # [TACGTA] # 12 3462 # # StyI: # [CCWWGG] # 464 3010 # # SwaI: # [ATTTAAAT] # 71 3403 # # TaiI: # [ACGT] # 10 55 100 106 135 151 # 151 173 174 242 274 379 # 1524 # # TaqI: # [TCGA] # 49 109 117 137 162 198 # 210 799 830 863 # # TaqII: # [CACCCA] # 639 745 2090 # # TatI: # [WGTACW] # 361 560 1088 1465 # # TauI: # [GCSGC] # 21 21 167 358 849 2058 # # TfiI: # [GAWTC] # 13 38 53 81 133 145 # 208 243 268 282 298 767 # 945 # # TseI: # [GCWGC] # 9 275 423 768 823 1176 # # TsoI: # [TARCCA] # 1255 2219 # # Tsp45I: # [GTSAC] # 137 257 454 581 927 1118 # # Tsp4CI*: # [ACNGT] # 21 173 251 289 363 972 # 1405 # # TspDTI: # [ATGAA] # 21 24 44 60 90 111 # 135 153 159 173 190 203 # 225 267 363 366 890 # # TspEI: # [AATT] # 11 14 14 14 21 31 # 34 39 40 42 50 61 # 72 75 75 78 78 103 # 133 159 165 170 406 463 # 509 617 # # TspGWI: # [ACGGA] # 175 393 532 2374 # # TspRI: # [CASTGNN] # 21 21 92 255 572 1123 # 1390 # # VspI: # [ATTAAT] # 701 724 2049 # # XcmI: # [CCANNNNNNNNNTGG] # 56 148 3270 # # XhoII: # [RGATCY] # 202 1322 1950 # # XmnI: # [GAANNNNTTC] # 520 1265 1689 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 3474 # Reported_sequences: 1 # Reported_hitcount: 928 #---------------------------------------