Restriction Fragments for YJL015C



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The currently selected gene/sequence is :

YJL015C on chromosome X from coordinates 407426 to 407142.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.30040.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 5 9 + Tsp45I GTSAC 4 9 . . 5 9 + MaeIII GTNAC 4 9 . . 8 11 + TaiI ACGT 11 7 . . 8 11 + SetI ASST 11 7 . . 8 11 + MaeII ACGT 8 10 . . 11 14 + MseI TTAA 11 13 . . 34 38 + FokI GGATG 47 51 . . 34 38 + BseGI GGATG 40 38 . . 35 39 - BccI CCATC 29 30 . . 80 83 + CviJI RGCY 81 81 . . 79 84 - TsoI TARCCA 69 67 . . 87 95 + AlwNI CAGNNNCTG 92 89 . . 92 96 + Tsp4CI* ACNGT 94 93 . . 95 98 + Csp6I GTAC 95 97 . . 95 98 + RsaI GTAC 96 96 . . 111 117 + TspRI CASTGNN 117 108 . . 112 116 + Tsp4CI* ACNGT 114 113 . . 136 139 + CviJI RGCY 137 137 . . 137 147 + MwoI GCNNNNNNNGC 143 140 . . 135 140 - TsoI TARCCA 125 123 . . 148 152 + MboII GAAGA 160 159 . . 157 160 + CviRI* TGCA 158 158 . . 158 163 + BsrDI GCAATG 165 163 . . 166 177 + BcgI CGANNNNNNTGC 155 153 189 187 169 174 + TatI WGTACW 169 173 . . 169 174 + ScaI AGTACT 171 171 . . 170 173 + Csp6I GTAC 170 172 . . 170 173 + RsaI GTAC 171 171 . . 177 181 + Hpy99I CGWCG 181 176 . . 187 190 + TaiI ACGT 190 186 . . 187 190 + SetI ASST 190 186 . . 187 190 + MaeII ACGT 187 189 . . 213 216 + FatI CATG 212 216 . . 213 216 + NlaIII CATG 216 212 . . 213 216 + CviAII CATG 213 215 . . 211 215 - TspDTI ATGAA 201 199 . . 220 225 + Hpy166II GTNNAC 222 222 . . 227 230 + CviRI* TGCA 228 228 . . 229 234 + MfeI CAATTG 229 233 . . 230 233 + TspEI AATT 229 233 . . 234 238 + TfiI GAWTC 234 237 . . 234 238 + HinfI GANTC 234 237 . . 236 240 - TspDTI ATGAA 226 224 . . 254 258 + Hpy188I TCNGA 256 255 . . 264 269 - BsrDI GCAATG 263 261 . . #--------------------------------------- # # # AlwNI: # [CAGNNNCTG] # 92 193 # # BccI: # [CCATC] # 29 256 # # BcgI: # [CGANNNNNNTGC] # 34 96 155 # # BseGI: # [GGATG] # 40 245 # # BsrDI: # [GCAATG] # 22 98 165 # # Csp6I: # [GTAC] # 75 95 115 # # CviAII: # [CATG] # 72 213 # # CviJI: # [RGCY] # 56 81 148 # # CviRI*: # [TGCA] # 57 70 158 # # FatI: # [CATG] # 73 212 # # FokI: # [GGATG] # 47 238 # # HinfI: # [GANTC] # 51 234 # # Hpy166II: # [GTNNAC] # 63 222 # # Hpy188I: # [TCNGA] # 29 256 # # Hpy99I: # [CGWCG] # 104 181 # # MaeII: # [ACGT] # 8 98 179 # # MaeIII: # [GTNAC] # 4 281 # # MboII: # [GAAGA] # 125 160 # # MfeI: # [CAATTG] # 56 229 # # MseI: # [TTAA] # 11 274 # # MwoI: # [GCNNNNNNNGC] # 142 143 # # NlaIII: # [CATG] # 69 216 # # RsaI: # [GTAC] # 75 96 114 # # ScaI: # [AGTACT] # 114 171 # # SetI: # [ASST] # 11 95 179 # # TaiI: # [ACGT] # 11 95 179 # # TatI: # [WGTACW] # 116 169 # # TfiI: # [GAWTC] # 51 234 # # TsoI: # [TARCCA] # 56 69 160 # # Tsp45I: # [GTSAC] # 4 281 # # Tsp4CI*: # [ACNGT] # 20 94 171 # # TspDTI: # [ATGAA] # 25 59 201 # # TspEI: # [AATT] # 56 229 # # TspRI: # [CASTGNN] # 117 168 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 285 # Reported_sequences: 1 # Reported_hitcount: 45 #---------------------------------------