Restriction Fragments for YIR036W-A



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

YIR036W-A on chromosome IX from coordinates 422635 to 423036.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.4120.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 10 13 + HpaII CCGG 10 12 . . 18 21 + MnlI CCTC 28 27 . . 37 40 + TaqI TCGA 37 39 . . 37 42 + Hpy178III* TCNNGA 38 40 . . 42 45 + SetI ASST 45 41 . . 39 43 - BsmAI GTCTC 33 37 . . 39 44 - Eco31I GGTCTC 33 37 . . 47 51 - TspGWI ACGGA 37 35 . . 56 59 + TaqI TCGA 56 58 . . 58 61 - MnlI CCTC 51 50 . . 70 81 + DrdI GACNNNNNNGTC 76 74 . . 73 78 + ApoI RAATTY 73 77 . . 74 77 + TspEI AATT 73 77 . . 82 85 + CviRI* TGCA 83 83 . . 85 89 + Tsp4CI* ACNGT 87 86 . . 81 86 - BsgI GTGCAG 66 64 . . 98 101 + CviRI* TGCA 99 99 . . 99 109 + MwoI GCNNNNNNNGC 105 102 . . 99 109 + BstAPI GCANNNNNTGC 105 102 . . 106 111 + PstI CTGCAG 110 106 . . 106 111 + SfeI* CTRYAG 106 110 . . 107 110 + CviRI* TGCA 108 108 . . 115 118 + SetI ASST 118 114 . . 115 118 + CviJI RGCY 116 116 . . 115 118 + AluI AGCT 116 116 . . 119 123 - TspGWI ACGGA 109 107 . . 136 141 + Hpy166II GTNNAC 138 138 . . 136 141 + AccI GTMKAC 137 139 . . 133 137 - BceAI ACGGC 118 120 . . 143 148 + EcoT22I ATGCAT 147 143 . . 144 147 + CviRI* TGCA 145 145 . . 142 146 - SfaNI GCATC 132 136 . . 145 150 - BsmI GAATGC 145 143 . . 148 152 - TspDTI ATGAA 138 136 . . 156 159 + MnlI CCTC 166 165 . . 157 162 + Ksp632I* CTCTTC 163 166 . . 161 164 + TaqI TCGA 161 163 . . 158 162 - MboII GAAGA 150 149 . . 167 171 + Tsp4CI* ACNGT 169 168 . . 177 187 + BsiYI* CCNNNNNNNGG 183 180 . . 178 183 + MfeI CAATTG 178 182 . . 174 178 - HphI GGTGA 166 165 . . 179 182 + TspEI AATT 178 182 . . 182 185 + CviRI* TGCA 183 183 . . 183 188 + Cac8I GCNNGC 185 185 . . 186 189 + CviJI RGCY 187 187 . . 186 189 + HaeIII GGCC 187 187 . . 193 203 + BsiYI* CCNNNNNNNGG 199 196 . . 194 199 + DsaI* CCRYGG 194 198 . . 194 199 + SecI* CCNNGG 194 198 . . 202 207 + NlaIV GGNNCC 204 204 . . 202 207 + HgiCI* GGYRCC 202 206 . . 200 203 - MnlI CCTC 193 192 . . 205 208 + SetI ASST 208 204 . . 221 224 + SetI ASST 224 220 . . 235 239 + Hin4II* CCTTC 245 244 . . 249 254 + PsiI TTATAA 251 251 . . 275 280 + Hpy178III* TCNNGA 276 278 . . 275 280 + NruI TCGCGA 277 277 . . 276 279 + FnuDII* CGCG 277 277 . . 282 285 + CviJI RGCY 283 283 . . 301 305 + Hin4II* CCTTC 311 310 . . 304 308 - MboII GAAGA 296 295 . . 296 308 - BarI GAAGNNNNNNTAC 320 315 288 283 315 318 + CviRI* TGCA 316 316 . . 329 334 + TsoI TARCCA 345 343 . . 330 333 + CviJI RGCY 331 331 . . 346 349 + SetI ASST 349 345 . . 366 369 - MnlI CCTC 359 358 . . 378 381 + FatI CATG 377 381 . . 378 381 + NlaIII CATG 381 377 . . 378 381 + CviAII CATG 378 380 . . 381 384 - MnlI CCTC 374 373 . . 386 390 + Tsp4CI* ACNGT 388 387 . . 399 402 + MseI TTAA 399 401 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 137 265 # # AluI: # [AGCT] # 116 286 # # ApoI: # [RAATTY] # 73 329 # # BarI: # [GAAGNNNNNNTAC] # 32 82 288 # # BceAI: # [ACGGC] # 118 284 # # BsgI: # [GTGCAG] # 66 336 # # BsiYI*: # [CCNNNNNNNGG] # 16 183 203 # # BsmAI: # [GTCTC] # 33 369 # # BsmI: # [GAATGC] # 145 257 # # BstAPI: # [GCANNNNNTGC] # 105 297 # # Cac8I: # [GCNNGC] # 185 217 # # CviAII: # [CATG] # 24 378 # # CviJI: # [RGCY] # 48 71 71 96 116 # # CviRI*: # [TGCA] # 9 16 37 38 83 86 # 133 # # DrdI: # [GACNNNNNNGTC] # 76 326 # # DsaI*: # [CCRYGG] # 194 208 # # Eco31I: # [GGTCTC] # 33 369 # # EcoT22I: # [ATGCAT] # 147 255 # # FatI: # [CATG] # 25 377 # # FnuDII*: # [CGCG] # 125 277 # # HaeIII: # [GGCC] # 187 215 # # HgiCI*: # [GGYRCC] # 200 202 # # Hin4II*: # [CCTTC] # 66 91 245 # # HpaII: # [CCGG] # 10 392 # # HphI: # [GGTGA] # 166 236 # # Hpy166II: # [GTNNAC] # 138 264 # # Hpy178III*: # [TCNNGA] # 38 126 238 # # Ksp632I*: # [CTCTTC] # 163 239 # # MboII: # [GAAGA] # 106 146 150 # # MfeI: # [CAATTG] # 178 224 # # MnlI: # [CCTC] # 15 23 27 28 28 115 # 166 # # MseI: # [TTAA] # 3 399 # # MwoI: # [GCNNNNNNNGC] # 105 297 # # NlaIII: # [CATG] # 21 381 # # NlaIV: # [GGNNCC] # 198 204 # # NruI: # [TCGCGA] # 125 277 # # PsiI: # [TTATAA] # 151 251 # # PstI: # [CTGCAG] # 110 292 # # SecI*: # [CCNNGG] # 194 208 # # SetI: # [ASST] # 16 45 53 73 90 125 # # SfaNI: # [GCATC] # 132 270 # # SfeI*: # [CTRYAG] # 106 296 # # TaqI: # [TCGA] # 19 37 105 241 # # TsoI: # [TARCCA] # 57 345 # # Tsp4CI*: # [ACNGT] # 14 82 87 219 # # TspDTI: # [ATGAA] # 138 264 # # TspEI: # [AATT] # 73 105 224 # # TspGWI: # [ACGGA] # 37 72 293 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 402 # Reported_sequences: 1 # Reported_hitcount: 75 #---------------------------------------