Restriction Fragments for DAL3/YIR032C



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The currently selected gene/sequence is :

DAL3/YIR032C on chromosome IX from coordinates 415617 to 415030.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.4154.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 3 9 + BstEII GGTNACC 3 8 . . 3 7 + HphI GGTGA 15 14 . . 4 8 + Tsp45I GTSAC 3 8 . . 4 8 + MaeIII GTNAC 3 8 . . 7 11 + Tsp4CI* ACNGT 9 8 . . 8 13 + DsaI* CCRYGG 8 12 . . 8 13 + SecI* CCNNGG 8 12 . . 15 20 + EciI GGCGGA 31 29 . . 16 19 - AciI CCGC 16 18 . . 19 23 - BsmAI GTCTC 13 17 . . 27 38 + DrdI GACNNNNNNGTC 33 31 . . 34 38 + PleI GAGTC 42 43 . . 34 38 + MlyI GAGTC 43 43 . . 34 38 + HinfI GANTC 34 37 . . 38 42 + Hin4II* CCTTC 48 47 . . 41 44 + TaqI TCGA 41 43 . . 43 48 + BseRI GAGGAG 58 56 . . 43 46 - MnlI CCTC 36 35 . . 52 56 + BslFI GGGAC 66 70 . . 60 63 + TspEI AATT 59 63 . . 71 75 + TspDTI ATGAA 86 84 . . 73 77 + MboII GAAGA 85 84 . . 73 78 - Ksp632I* CTCTTC 68 71 . . 86 90 + FokI GGATG 99 103 . . 86 90 + BseGI GGATG 92 90 . . 89 92 + CviRI* TGCA 90 90 . . 87 91 - SfaNI GCATC 77 81 . . 95 98 + SetI ASST 98 94 . . 105 108 + SetI ASST 108 104 . . 108 111 + CviRI* TGCA 109 109 . . 114 118 + BssKI CCNGG 113 118 . . 114 118 + BseBI CCWGG 115 116 . . 114 119 + SecI* CCNNGG 114 118 . . 114 118 + ScrFI CCNGG 115 116 . . 114 118 + EcoRII CCWGG 113 118 . . 125 136 + BcgI CGANNNNNNTGC 114 112 148 146 126 129 + DpnI GATC 127 127 . . 126 129 + BstKTI GATC 128 126 . . 126 129 + MboI GATC 125 129 . . 131 134 + TspEI AATT 130 134 . . 145 148 + CviJI RGCY 146 146 . . 147 151 + BssKI CCNGG 146 151 . . 147 151 + BseBI CCWGG 148 149 . . 147 151 + ScrFI CCNGG 148 149 . . 147 151 + EcoRII CCWGG 146 151 . . 149 152 + SetI ASST 152 148 . . 154 164 - Hin4I GAYNNNNNVTC 177 172 145 140 167 171 - BsrI ACTGG 167 165 . . 180 185 + MfeI CAATTG 180 184 . . 181 184 + TspEI AATT 180 184 . . 185 190 + NlaIV GGNNCC 187 187 . . 186 195 + XmnI GAANNNNTTC 190 190 . . 188 191 + SetI ASST 191 187 . . 194 198 - TspGWI ACGGA 184 182 . . 209 212 + CviJI RGCY 210 210 . . 210 214 + TauI GCSGC 213 210 . . 210 214 + BlsI GCNGC 212 211 . . 210 214 + BisI GCNGC 211 212 . . 211 214 + AciI CCGC 211 213 . . 215 218 + SetI ASST 218 214 . . 233 238 + SmlI CTYRAG 233 237 . . 238 241 + CviJI RGCY 239 239 . . 233 238 - Bce83I* CTTGAG 218 216 . . 238 243 + NlaIV GGNNCC 240 240 . . 273 276 + MnlI CCTC 283 282 . . 275 278 + TaqI TCGA 275 277 . . 282 286 + SfaNI GCATC 291 295 . . 283 287 - FokI GGATG 269 273 . . 283 287 - BseGI GGATG 282 280 . . 285 289 - TspGWI ACGGA 275 273 . . 293 296 + Csp6I GTAC 293 295 . . 293 296 + RsaI GTAC 294 294 . . 301 304 + TaiI ACGT 304 300 . . 301 304 + SetI ASST 304 300 . . 301 304 + MaeII ACGT 301 303 . . 322 325 + TaiI ACGT 325 321 . . 322 325 + SetI ASST 325 321 . . 322 325 + MaeII ACGT 322 324 . . 326 331 + NspBII* CMGCKG 328 328 . . 328 338 + MwoI GCNNNNNNNGC 334 331 . . 328 331 - AciI CCGC 328 330 . . 363 366 + TspEI AATT 362 366 . . 373 383 + FalI AAGNNNNNCTT 364 359 396 391 374 377 + CviJI RGCY 375 375 . . 373 383 - FalI AAGNNNNNCTT 396 391 364 359 384 389 + Hpy166II GTNNAC 386 386 . . 384 389 + AccI GTMKAC 385 387 . . 388 391 + TaiI ACGT 391 387 . . 388 391 + SetI ASST 391 387 . . 388 391 + MaeII ACGT 388 390 . . 393 396 - MnlI CCTC 386 385 . . 396 399 + CviJI RGCY 397 397 . . 403 408 + BspLU11I* ACATGT 403 407 . . 403 408 + NspI RCATGY 407 403 . . 403 408 + AflIII ACRYGT 403 407 . . 404 407 + FatI CATG 403 407 . . 404 407 + NlaIII CATG 407 403 . . 404 407 + CviAII CATG 404 406 . . 420 423 + CviJI RGCY 421 421 . . 420 423 + HaeIII GGCC 421 421 . . 424 428 + MaeIII GTNAC 423 428 . . 421 425 - BceAI ACGGC 406 408 . . 427 430 + SetI ASST 430 426 . . 433 436 + CviJI RGCY 434 434 . . 435 439 + DdeI CTNAG 435 438 . . 438 441 + SetI ASST 441 437 . . 439 444 + KpnI GGTACC 443 439 . . 439 444 + NlaIV GGNNCC 441 441 . . 439 444 + HgiCI* GGYRCC 439 443 . . 439 444 + Acc65I GGTACC 439 443 . . 440 443 + Csp6I GTAC 440 442 . . 434 440 - Bpu10I CCTNAGC 435 438 . . 440 443 + RsaI GTAC 441 441 . . 442 445 + SetI ASST 445 441 . . 443 447 + BssKI CCNGG 442 447 . . 443 447 + BseBI CCWGG 444 445 . . 443 447 + ScrFI CCNGG 444 445 . . 443 447 + EcoRII CCWGG 442 447 . . 447 452 + NspI RCATGY 451 447 . . 447 452 + Cac8I GCNNGC 449 449 . . 447 452 + SphI GCATGC 451 447 . . 448 451 + FatI CATG 447 451 . . 448 451 + NlaIII CATG 451 447 . . 448 451 + CviAII CATG 448 450 . . 451 454 + Hin6I GCGC 451 453 . . 451 454 + GlaI GCGC 452 452 . . 451 454 + HhaI GCGC 453 451 . . 456 459 + FatI CATG 455 459 . . 456 459 + NlaIII CATG 459 455 . . 456 459 + CviAII CATG 456 458 . . 462 467 + TatI WGTACW 462 466 . . 462 467 + ScaI AGTACT 464 464 . . 463 466 + Csp6I GTAC 463 465 . . 463 466 + RsaI GTAC 464 464 . . 475 479 + MboII GAAGA 487 486 . . 499 502 + MseI TTAA 499 501 . . 513 518 + Hpy178III* TCNNGA 514 516 . . 516 521 + NlaIV GGNNCC 518 518 . . 516 520 + BmgT120I GGNCC 517 518 . . 516 520 + AvaII GGWCC 516 519 . . 516 520 + AsuI* GGNCC 516 519 . . 527 530 + CviJI RGCY 528 528 . . 527 530 + HaeIII GGCC 528 528 . . 527 531 + BmgT120I GGNCC 528 529 . . 527 531 + AsuI* GGNCC 527 530 . . 529 534 + BmeT110I CYCGRG 530 532 . . 529 534 + AvaI CYCGRG 529 533 . . 534 538 - Hin4II* CCTTC 528 527 . . 539 543 + Tsp4CI* ACNGT 541 540 . . 547 551 + MboII GAAGA 559 558 . . 555 560 + SfeI* CTRYAG 555 559 . . 560 565 + BtgZI GCGATG 575 579 . . 562 566 - BccI CCATC 556 557 . . 567 571 + Hpy99I CGWCG 571 566 . . 569 572 + TaiI ACGT 572 568 . . 569 572 + SetI ASST 572 568 . . 569 572 + MaeII ACGT 569 571 . . #--------------------------------------- # # # Acc65I: # [GGTACC] # 149 439 # # AccI: # [GTMKAC] # 203 385 # # AciI: # [CCGC] # 16 117 195 260 # # AflIII: # [ACRYGT] # 185 403 # # AsuI*: # [GGNCC] # 11 61 516 # # AvaI: # [CYCGRG] # 59 529 # # AvaII: # [GGWCC] # 72 516 # # BccI: # [CCATC] # 32 556 # # Bce83I*: # [CTTGAG] # 218 370 # # BceAI: # [ACGGC] # 182 406 # # BcgI: # [CGANNNNNNTGC] # 34 114 440 # # BisI: # [GCNGC] # 211 377 # # BlsI: # [GCNGC] # 212 376 # # BmeT110I: # [CYCGRG] # 58 530 # # BmgT120I: # [GGNCC] # 11 60 517 # # Bpu10I: # [CCTNAGC] # 153 435 # # BseBI: # [CCWGG] # 33 115 144 296 # # BseGI: # [GGATG] # 92 190 306 # # BseRI: # [GAGGAG] # 58 530 # # BslFI: # [GGGAC] # 66 522 # # BsmAI: # [GTCTC] # 13 575 # # BspLU11I*: # [ACATGT] # 185 403 # # BsrI: # [ACTGG] # 167 421 # # BssKI: # [CCNGG] # 33 113 146 296 # # BstEII: # [GGTNACC] # 3 585 # # BstKTI: # [GATC] # 128 460 # # BtgZI: # [GCGATG] # 13 575 # # Cac8I: # [GCNNGC] # 139 449 # # Csp6I: # [GTAC] # 23 125 147 293 # # CviAII: # [CATG] # 8 44 132 404 # # CviJI: # [RGCY] # 13 22 24 29 60 64 # 94 136 146 # # CviRI*: # [TGCA] # 19 90 479 # # DdeI: # [CTNAG] # 153 435 # # DpnI: # [GATC] # 127 461 # # DrdI: # [GACNNNNNNGTC] # 33 555 # # DsaI*: # [CCRYGG] # 8 580 # # EciI: # [GGCGGA] # 31 557 # # EcoRII: # [CCWGG] # 33 113 146 296 # # FalI: # [AAGNNNNNCTT] # 0 0 32 192 364 # # FatI: # [CATG] # 8 44 133 403 # # FokI: # [GGATG] # 99 170 319 # # GlaI: # [GCGC] # 136 452 # # HaeIII: # [GGCC] # 60 107 421 # # HgiCI*: # [GGYRCC] # 149 439 # # HhaI: # [GCGC] # 135 453 # # Hin4I: # [GAYNNNNNVTC] # 32 145 411 # # Hin4II*: # [CCTTC] # 48 60 480 # # Hin6I: # [GCGC] # 137 451 # # HinfI: # [GANTC] # 34 554 # # HphI: # [GGTGA] # 15 573 # # Hpy166II: # [GTNNAC] # 202 386 # # Hpy178III*: # [TCNNGA] # 74 514 # # Hpy99I: # [CGWCG] # 17 571 # # KpnI: # [GGTACC] # 145 443 # # Ksp632I*: # [CTCTTC] # 68 520 # # MaeII: # [ACGT] # 19 21 66 181 301 # # MaeIII: # [GTNAC] # 3 165 420 # # MboI: # [GATC] # 125 463 # # MboII: # [GAAGA] # 29 72 85 402 # # MfeI: # [CAATTG] # 180 408 # # MlyI: # [GAGTC] # 43 545 # # MnlI: # [CCTC] # 36 103 202 247 # # MseI: # [TTAA] # 89 499 # # MwoI: # [GCNNNNNNNGC] # 254 334 # # NlaIII: # [CATG] # 8 44 129 407 # # NlaIV: # [GGNNCC] # 53 70 77 187 201 # # NspBII*: # [CMGCKG] # 260 328 # # NspI: # [RCATGY] # 44 137 407 # # PleI: # [GAGTC] # 42 546 # # RsaI: # [GTAC] # 23 124 147 294 # # ScaI: # [AGTACT] # 124 464 # # ScrFI: # [CCNGG] # 33 115 144 296 # # SecI*: # [CCNNGG] # 8 106 474 # # SetI: # [ASST] # 4 10 11 16 21 27 # 39 39 44 66 86 98 # 127 # # SfaNI: # [GCATC] # 77 214 297 # # SfeI*: # [CTRYAG] # 33 555 # # SmlI: # [CTYRAG] # 233 355 # # SphI: # [GCATGC] # 137 451 # # TaiI: # [ACGT] # 16 21 66 181 304 # # TaqI: # [TCGA] # 41 234 313 # # TatI: # [WGTACW] # 126 462 # # TauI: # [GCSGC] # 213 375 # # Tsp45I: # [GTSAC] # 3 585 # # Tsp4CI*: # [ACNGT] # 9 47 532 # # TspDTI: # [ATGAA] # 86 502 # # TspEI: # [AATT] # 50 59 71 182 226 # # TspGWI: # [ACGGA] # 91 184 313 # # XmnI: # [GAANNNNTTC] # 190 398 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 588 # Reported_sequences: 1 # Reported_hitcount: 157 #---------------------------------------