Restriction Fragments for YVH1/YIR026C



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The currently selected gene/sequence is :

YVH1/YIR026C on chromosome IX from coordinates 405967 to 404873.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.27734.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 3 6 + CviJI RGCY 4 4 . . 4 14 + MwoI GCNNNNNNNGC 10 7 . . 12 15 + CviRI* TGCA 13 13 . . 18 22 + DdeI CTNAG 18 21 . . 18 22 + BspCNI CTCAG 31 29 . . 18 22 + BseMII CTCAG 32 30 . . 22 27 + Hpy166II GTNNAC 24 24 . . 22 27 + AccI GTMKAC 23 25 . . 28 31 - MnlI CCTC 21 20 . . 34 38 + MaeIII GTNAC 33 38 . . 38 42 + BssKI CCNGG 37 42 . . 38 42 + BseBI CCWGG 39 40 . . 38 42 + ScrFI CCNGG 39 40 . . 38 42 + EcoRII CCWGG 37 42 . . 50 53 - MnlI CCTC 43 42 . . 64 69 + EciI GGCGGA 80 78 . . 68 72 + TfiI GAWTC 68 71 . . 68 72 + HinfI GANTC 68 71 . . 65 68 - AciI CCGC 65 67 . . 71 75 - TspGWI ACGGA 61 59 . . 78 81 + TspEI AATT 77 81 . . 101 106 + BsgI GTGCAG 122 120 . . 102 105 + CviRI* TGCA 103 103 . . 98 103 - TaqII CACCCA 88 86 . . 106 111 + ApoI RAATTY 106 110 . . 107 110 + TspEI AATT 106 110 . . 110 113 + MseI TTAA 110 112 . . 139 144 + ApoI RAATTY 139 143 . . 140 143 + TspEI AATT 139 143 . . 144 148 + BssKI CCNGG 143 148 . . 144 148 + BseBI CCWGG 145 146 . . 144 148 + ScrFI CCNGG 145 146 . . 144 148 + EcoRII CCWGG 143 148 . . 146 149 + SetI ASST 149 145 . . 153 158 + Hpy178III* TCNNGA 154 156 . . 174 177 + SetI ASST 177 173 . . 176 180 + MaeIII GTNAC 175 180 . . 197 201 + BccI CCATC 205 206 . . 199 204 + ClaI ATCGAT 200 202 . . 200 203 + TaqI TCGA 200 202 . . 214 218 + Tsp45I GTSAC 213 218 . . 214 218 + MaeIII GTNAC 213 218 . . 225 229 + BlsI GCNGC 227 226 . . 225 229 + BisI GCNGC 226 227 . . 225 229 + TseI GCWGC 225 228 . . 227 230 + CviRI* TGCA 228 228 . . 225 229 - BbvI GCAGC 212 216 . . 236 239 + TaqI TCGA 236 238 . . 239 243 + TspDTI ATGAA 254 252 . . 246 255 + XmnI GAANNNNTTC 250 250 . . 251 255 + TfiI GAWTC 251 254 . . 251 255 + HinfI GANTC 251 254 . . 253 257 - TspDTI ATGAA 243 241 . . 258 263 + BclI TGATCA 258 262 . . 259 262 + DpnI GATC 260 260 . . 259 262 + BstKTI GATC 261 259 . . 259 262 + MboI GATC 258 262 . . 278 282 + TspDTI ATGAA 293 291 . . 311 316 + Hpy178III* TCNNGA 312 314 . . 315 319 + MboII GAAGA 327 326 . . 322 332 + BsiYI* CCNNNNNNNGG 328 325 . . 322 332 + PflMI CCANNNNNTGG 328 325 . . 323 331 + DraIII CACNNNGTG 328 325 . . 327 330 + TaiI ACGT 330 326 . . 327 330 + SetI ASST 330 326 . . 327 330 + MaeII ACGT 327 329 . . 334 344 + MwoI GCNNNNNNNGC 340 337 . . 359 363 + HinfI GANTC 359 362 . . 362 367 + Hpy178III* TCNNGA 363 365 . . 363 368 + SmlI CTYRAG 363 367 . . 359 363 - PleI GAGTC 353 354 . . 363 368 + XhoI CTCGAG 363 367 . . 359 363 - MlyI GAGTC 353 353 . . 363 368 + BmeT110I CYCGRG 364 366 . . 363 368 + AvaI CYCGRG 363 367 . . 364 367 + TaqI TCGA 364 366 . . 364 369 + Hpy178III* TCNNGA 365 367 . . 355 366 - BcgI CGANNNNNNTGC 378 376 344 342 367 372 + XhoII RGATCY 367 371 . . 367 372 + BglII AGATCT 367 371 . . 368 371 + DpnI GATC 369 369 . . 368 371 + BstKTI GATC 370 368 . . 368 371 + MboI GATC 367 371 . . 373 377 + MaeIII GTNAC 372 377 . . 376 379 + SetI ASST 379 375 . . 377 381 + Hin4II* CCTTC 387 386 . . 379 383 - TspDTI ATGAA 369 367 . . 387 390 + CviJI RGCY 388 388 . . 392 395 + SetI ASST 395 391 . . 414 418 + Tsp45I GTSAC 413 418 . . 414 418 + MaeIII GTNAC 413 418 . . 426 429 + CviJI RGCY 427 427 . . 427 437 + MwoI GCNNNNNNNGC 433 430 . . 425 430 - TsoI TARCCA 415 413 . . 431 434 + CviRI* TGCA 432 432 . . 432 437 + Cac8I GCNNGC 434 434 . . 440 445 + Hpy178III* TCNNGA 441 443 . . 444 447 - MnlI CCTC 437 436 . . 447 451 + MboII GAAGA 459 458 . . 464 467 + CviJI RGCY 465 465 . . 474 479 + ApoI RAATTY 474 478 . . 475 478 + TspEI AATT 474 478 . . 480 483 + FatI CATG 479 483 . . 480 483 + NlaIII CATG 483 479 . . 480 483 + CviAII CATG 480 482 . . 478 482 - TspDTI ATGAA 468 466 . . 488 491 + TspEI AATT 487 491 . . 509 514 - TaqII CACCCA 499 497 . . 524 527 + TaqI TCGA 524 526 . . 514 524 - BsaXI ACNNNNNCTCC 536 533 506 503 515 525 - Hin4I GAYNNNNNVTC 538 533 506 501 530 533 + TaqI TCGA 530 532 . . 537 542 + GsaI CCCAGC 541 537 . . 537 542 + BseYI CCCAGC 537 541 . . 540 543 + CviJI RGCY 541 541 . . 549 554 + BtsI GCAGTG 556 554 . . 549 559 + MwoI GCNNNNNNNGC 555 552 . . 550 556 + TspRI CASTGNN 556 547 . . 557 560 + SetI ASST 560 556 . . 557 560 + CviJI RGCY 558 558 . . 557 560 + AluI AGCT 558 558 . . 559 564 + Eco57MI CTGRAG 580 578 . . 559 564 + Eco57I CTGAAG 580 578 . . 579 584 + XhoII RGATCY 579 583 . . 580 583 + DpnI GATC 581 581 . . 580 583 + BstKTI GATC 582 580 . . 580 583 + MboI GATC 579 583 . . 583 587 + BccI CCATC 591 592 . . 580 584 - BinI* GGATC 574 575 . . 590 594 + BlsI GCNGC 592 591 . . 590 594 + BisI GCNGC 591 592 . . 590 594 + BbvI GCAGC 602 606 . . 590 594 + TseI GCWGC 590 593 . . 596 599 + TspEI AATT 595 599 . . 600 605 + Eco31I GGTCTC 606 610 . . 601 605 + BsmAI GTCTC 606 610 . . 607 610 + TspEI AATT 606 610 . . 610 615 + Hpy178III* TCNNGA 611 613 . . 619 624 + AhaIII* TTTAAA 621 621 . . 620 623 + MseI TTAA 620 622 . . 625 629 + TfiI GAWTC 625 628 . . 625 629 + HinfI GANTC 625 628 . . 625 635 + Hin4I GAYNNNNNVTC 616 611 648 643 628 632 + Hpy188I TCNGA 630 629 . . 631 635 + PleI GAGTC 639 640 . . 631 635 + MlyI GAGTC 640 640 . . 631 635 + HinfI GANTC 631 634 . . 635 639 + Hpy99I CGWCG 639 634 . . 625 635 - Hin4I GAYNNNNNVTC 648 643 616 611 653 656 + TspEI AATT 652 656 . . 655 658 + MseI TTAA 655 657 . . 659 664 + Eco57MI CTGRAG 680 678 . . 659 664 + Eco57I CTGAAG 680 678 . . 663 666 + SetI ASST 666 662 . . 663 666 + CviJI RGCY 664 664 . . 663 666 + AluI AGCT 664 664 . . 665 669 + DdeI CTNAG 665 668 . . 669 673 + MboII GAAGA 681 680 . . 665 669 - BspCNI CTCAG 657 655 . . 665 669 - BseMII CTCAG 656 654 . . 669 674 - Ksp632I* CTCTTC 664 667 . . 669 675 - SapI GCTCTTC 664 667 . . 677 680 + SetI ASST 680 676 . . 678 684 + BstEII GGTNACC 678 683 . . 679 683 + Tsp45I GTSAC 678 683 . . 679 683 + MaeIII GTNAC 678 683 . . 681 690 + MslI CAYNNNNRTG 685 685 . . 681 690 + OliI CACNNNNGTG 685 685 . . 683 686 + AciI CCGC 683 685 . . 680 684 - HphI GGTGA 672 671 . . 685 690 + BtsI GCAGTG 692 690 . . 686 692 + TspRI CASTGNN 692 683 . . 702 707 + BsgI GTGCAG 723 721 . . 703 706 + CviRI* TGCA 704 704 . . 713 716 + TspEI AATT 712 716 . . 733 737 - TspDTI ATGAA 723 721 . . 738 741 + FnuDII* CGCG 739 739 . . 739 742 + Hin6I GCGC 739 741 . . 739 742 + GlaI GCGC 740 740 . . 739 742 + HhaI GCGC 741 739 . . 735 740 - BtgZI GCGATG 720 724 . . 742 745 + FatI CATG 741 745 . . 742 745 + NlaIII CATG 745 741 . . 742 745 + CviAII CATG 742 744 . . 760 764 + TfiI GAWTC 760 763 . . 760 764 + HinfI GANTC 760 763 . . 766 770 + Hpy188I TCNGA 768 767 . . 771 775 + BslFI GGGAC 785 789 . . 769 772 - MnlI CCTC 762 761 . . 773 783 + Hin4I GAYNNNNNVTC 764 759 796 791 778 782 - TspDTI ATGAA 768 766 . . 782 787 + Hpy178III* TCNNGA 783 785 . . 773 783 - Hin4I GAYNNNNNVTC 796 791 764 759 788 792 + BmgT120I GGNCC 789 790 . . 788 792 + AsuI* GGNCC 788 791 . . 786 789 - MnlI CCTC 779 778 . . 789 792 + CviJI RGCY 790 790 . . 789 792 + HaeIII GGCC 790 790 . . 790 794 + TauI GCSGC 793 790 . . 790 794 + BlsI GCNGC 792 791 . . 790 794 + BisI GCNGC 791 792 . . 791 794 + AciI CCGC 791 793 . . 801 805 + BccI CCATC 809 810 . . 819 824 + Hpy178III* TCNNGA 820 822 . . 823 827 + PleI GAGTC 831 832 . . 823 827 + MlyI GAGTC 832 832 . . 823 827 + HinfI GANTC 823 826 . . 825 829 + Tsp45I GTSAC 824 829 . . 825 829 + MaeIII GTNAC 824 829 . . 835 838 + TspEI AATT 834 838 . . 850 854 - TspDTI ATGAA 840 838 . . 854 857 + TaqI TCGA 854 856 . . 858 861 + SetI ASST 861 857 . . 861 866 + AhaIII* TTTAAA 863 863 . . 862 865 + MseI TTAA 862 864 . . 869 873 + FokI GGATG 882 886 . . 869 873 + BseGI GGATG 875 873 . . 872 875 + CviRI* TGCA 873 873 . . 870 874 - SfaNI GCATC 860 864 . . 876 879 + SetI ASST 879 875 . . 890 895 + Cac8I GCNNGC 892 892 . . 904 908 - Hin4II* CCTTC 898 897 . . 917 920 + FatI CATG 916 920 . . 917 920 + NlaIII CATG 920 916 . . 917 920 + CviAII CATG 917 919 . . 915 919 - TspDTI ATGAA 905 903 . . 922 926 + BssKI CCNGG 921 926 . . 922 926 + BseBI CCWGG 923 924 . . 922 926 + ScrFI CCNGG 923 924 . . 922 926 + EcoRII CCWGG 921 926 . . 925 928 + CviJI RGCY 926 926 . . 947 951 + MaeIII GTNAC 946 951 . . 944 947 - AciI CCGC 944 946 . . 953 957 + BsrI ACTGG 958 956 . . 961 966 + XhoII RGATCY 961 965 . . 961 965 + BinI* GGATC 969 970 . . 962 965 + DpnI GATC 963 963 . . 962 965 + BstKTI GATC 964 962 . . 962 965 + MboI GATC 961 965 . . 965 968 + MaeI CTAG 965 967 . . 967 970 + SetI ASST 970 966 . . 969 974 + BsgI GTGCAG 990 988 . . 970 973 + CviRI* TGCA 971 971 . . 990 994 - FokI GGATG 976 980 . . 994 998 + FauI CCCGC 1002 1004 . . 990 994 - BseGI GGATG 989 987 . . 995 998 + AciI CCGC 995 997 . . 1003 1009 + AarI CACCTGC 1013 1017 . . 1004 1007 + SetI ASST 1007 1003 . . 1004 1009 + BspMI ACCTGC 1013 1017 . . 1007 1010 + CviRI* TGCA 1008 1008 . . 1012 1017 + SpeI ACTAGT 1012 1016 . . 1013 1016 + MaeI CTAG 1013 1015 . . 1023 1027 + BinI* GGATC 1031 1032 . . 1024 1027 + DpnI GATC 1025 1025 . . 1024 1027 + BstKTI GATC 1026 1024 . . 1024 1027 + MboI GATC 1023 1027 . . 1028 1031 + TspEI AATT 1027 1031 . . 1044 1050 + TspRI CASTGNN 1050 1041 . . 1044 1049 - BtsI GCAGTG 1043 1041 . . 1059 1062 + FatI CATG 1058 1062 . . 1059 1062 + NlaIII CATG 1062 1058 . . 1059 1062 + CviAII CATG 1059 1061 . . 1064 1067 + MseI TTAA 1064 1066 . . 1061 1066 - TsoI TARCCA 1051 1049 . . 1066 1069 + TspEI AATT 1065 1069 . . 1072 1076 + TfiI GAWTC 1072 1075 . . 1072 1076 + HinfI GANTC 1072 1075 . . 1075 1079 + Hpy188I TCNGA 1077 1076 . . #--------------------------------------- # # # AarI: # [CACCTGC] # 82 1013 # # AccI: # [GTMKAC] # 23 1072 # # AciI: # [CCGC] # 51 65 100 108 153 618 # # AhaIII*: # [TTTAAA] # 232 242 621 # # AluI: # [AGCT] # 106 431 558 # # ApoI: # [RAATTY] # 33 106 335 621 # # AsuI*: # [GGNCC] # 307 788 # # AvaI: # [CYCGRG] # 363 732 # # BbvI: # [GCAGC] # 212 390 493 # # BccI: # [CCATC] # 205 218 286 386 # # BcgI: # [CGANNNNNNTGC] # 34 344 717 # # BclI: # [TGATCA] # 258 837 # # BglII: # [AGATCT] # 367 728 # # BinI*: # [GGATC] # 62 64 395 574 # # BisI: # [GCNGC] # 200 226 304 365 # # BlsI: # [GCNGC] # 200 227 303 365 # # BmeT110I: # [CYCGRG] # 364 731 # # BmgT120I: # [GGNCC] # 306 789 # # BsaXI: # [ACNNNNNCTCC] # 30 506 559 # # BseBI: # [CCWGG] # 39 106 172 778 # # BseGI: # [GGATG] # 106 114 875 # # BseMII: # [CTCAG] # 32 439 624 # # BseYI: # [CCCAGC] # 537 558 # # BsgI: # [GTGCAG] # 105 122 267 601 # # BsiYI*: # [CCNNNNNNNGG] # 328 767 # # BslFI: # [GGGAC] # 310 785 # # BsmAI: # [GTCTC] # 489 606 # # BspCNI: # [CTCAG] # 31 438 626 # # BspMI: # [ACCTGC] # 82 1013 # # BsrI: # [ACTGG] # 137 958 # # BssKI: # [CCNGG] # 37 106 174 778 # # BstEII: # [GGTNACC] # 417 678 # # BstKTI: # [GATC] # 62 69 109 212 261 382 # # BtgZI: # [GCGATG] # 375 720 # # BtsI: # [GCAGTG] # 52 136 351 556 # # Cac8I: # [GCNNGC] # 203 434 458 # # ClaI: # [ATCGAT] # 200 895 # # CviAII: # [CATG] # 36 142 175 262 480 # # CviJI: # [RGCY] # 4 17 38 39 76 106 # 126 136 169 384 # # CviRI*: # [TGCA] # 13 37 87 90 98 125 # 169 204 272 # # DdeI: # [CTNAG] # 18 430 647 # # DpnI: # [GATC] # 62 70 109 212 260 382 # # DraIII: # [CACNNNGTG] # 328 767 # # EciI: # [GGCGGA] # 80 1015 # # Eco31I: # [GGTCTC] # 489 606 # # Eco57I: # [CTGAAG] # 100 415 580 # # Eco57MI: # [CTGRAG] # 100 415 580 # # EcoRII: # [CCWGG] # 37 106 174 778 # # FatI: # [CATG] # 37 142 175 262 479 # # FauI: # [CCCGC] # 93 1002 # # FnuDII*: # [CGCG] # 356 739 # # FokI: # [GGATG] # 94 119 882 # # GlaI: # [GCGC] # 355 740 # # GsaI: # [CCCAGC] # 541 554 # # HaeIII: # [GGCC] # 305 790 # # HhaI: # [GCGC] # 354 741 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 32 32 # 32 78 116 299 506 # # Hin4II*: # [CCTTC] # 197 387 511 # # Hin6I: # [GCGC] # 356 739 # # HinfI: # [GANTC] # 6 23 63 68 108 129 # 183 249 266 # # HphI: # [GGTGA] # 423 672 # # Hpy166II: # [GTNNAC] # 24 1071 # # Hpy178III*: # [TCNNGA] # 2 37 51 76 154 158 # 170 172 275 # # Hpy188I: # [TCNGA] # 18 138 309 630 # # Hpy99I: # [CGWCG] # 456 639 # # Ksp632I*: # [CTCTTC] # 431 664 # # MaeI: # [CTAG] # 48 82 965 # # MaeII: # [ACGT] # 327 768 # # MaeIII: # [GTNAC] # 33 38 41 122 142 146 # 149 159 265 # # MboI: # [GATC] # 62 72 109 212 258 382 # # MboII: # [GAAGA] # 132 222 327 414 # # MlyI: # [GAGTC] # 192 263 287 353 # # MnlI: # [CCTC] # 17 21 22 316 325 394 # # MseI: # [TTAA] # 31 35 110 202 207 510 # # MslI: # [CAYNNNNRTG] # 410 685 # # MwoI: # [GCNNNNNNNGC] # 10 93 122 330 540 # # NlaIII: # [CATG] # 33 142 175 262 483 # # OliI: # [CACNNNNGTG] # 410 685 # # PflMI: # [CCANNNNNTGG] # 328 767 # # PleI: # [GAGTC] # 192 264 286 353 # # SapI: # [GCTCTTC] # 431 664 # # ScrFI: # [CCNGG] # 39 106 172 778 # # SetI: # [ASST] # 14 16 18 28 37 49 # 88 91 106 149 153 165 # 181 # # SfaNI: # [GCATC] # 235 860 # # SmlI: # [CTYRAG] # 363 732 # # SpeI: # [ACTAGT] # 83 1012 # # TaiI: # [ACGT] # 330 765 # # TaqI: # [TCGA] # 6 36 128 160 200 241 # 324 # # TaqII: # [CACCCA] # 88 411 596 # # TauI: # [GCSGC] # 302 793 # # TfiI: # [GAWTC] # 23 68 135 183 312 374 # # TseI: # [GCWGC] # 225 365 505 # # TsoI: # [TARCCA] # 44 415 636 # # Tsp45I: # [GTSAC] # 146 200 213 265 271 # # TspDTI: # [ATGAA] # 11 39 45 65 72 76 # 99 190 243 255 # # TspEI: # [AATT] # 11 13 29 30 33 38 # 46 60 77 108 122 193 # 335 # # TspGWI: # [ACGGA] # 61 1034 # # TspRI: # [CASTGNN] # 45 136 358 556 # # XhoI: # [CTCGAG] # 363 732 # # XhoII: # [RGATCY] # 134 212 367 382 # # XmnI: # [GAANNNNTTC] # 250 845 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1095 # Reported_sequences: 1 # Reported_hitcount: 269 #---------------------------------------