Restriction Fragments for YIR017W-A



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

YIR017W-A on chromosome IX from coordinates 383566 to 384165.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.9977.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 7 12 + Esp3I CGTCTC 13 17 . . 8 12 + BsmAI GTCTC 13 17 . . 13 16 + MseI TTAA 13 15 . . 28 31 + TspEI AATT 27 31 . . 37 47 + Hin4I GAYNNNNNVTC 28 23 60 55 40 43 + MseI TTAA 40 42 . . 49 52 + MseI TTAA 49 51 . . 61 64 + SetI ASST 64 60 . . 61 64 + CviJI RGCY 62 62 . . 61 64 + AluI AGCT 62 62 . . 93 98 + MfeI CAATTG 93 97 . . 94 97 + TspEI AATT 93 97 . . 106 111 + McrI* CGRYCG 109 107 . . 106 111 + PvuI CGATCG 109 107 . . 107 110 + DpnI GATC 108 108 . . 107 110 + BstKTI GATC 109 107 . . 107 110 + MboI GATC 106 110 . . 109 114 + Hpy178III* TCNNGA 110 112 . . 109 114 + NruI TCGCGA 111 111 . . 110 113 + FnuDII* CGCG 111 111 . . 134 137 + MseI TTAA 134 136 . . 141 146 + PstI CTGCAG 145 141 . . 141 146 + SfeI* CTRYAG 141 145 . . 142 145 + CviRI* TGCA 143 143 . . 143 147 + BlsI GCNGC 145 144 . . 143 147 + BisI GCNGC 144 145 . . 143 147 + BbvI GCAGC 155 159 . . 143 147 + TseI GCWGC 143 146 . . 145 148 + SetI ASST 148 144 . . 145 148 + CviJI RGCY 146 146 . . 145 148 + AluI AGCT 146 146 . . 153 156 + FatI CATG 152 156 . . 153 156 + NlaIII CATG 156 152 . . 153 156 + CviAII CATG 153 155 . . 151 155 - TspDTI ATGAA 141 139 . . 163 168 + ApoI RAATTY 163 167 . . 164 167 + TspEI AATT 163 167 . . 182 187 + BsmI GAATGC 188 186 . . 188 193 + NlaIV GGNNCC 190 190 . . 186 189 - AciI CCGC 186 188 . . 192 197 + NspBII* CMGCKG 194 194 . . 192 195 + AciI CCGC 192 194 . . 194 198 + BlsI GCNGC 196 195 . . 194 198 + BisI GCNGC 195 196 . . 194 198 + TseI GCWGC 194 197 . . 198 202 + Hpy99I CGWCG 202 197 . . 194 198 - BbvI GCAGC 181 185 . . 199 203 + HgaI GACGC 208 213 . . 199 204 + AcyI GRCGYC 200 202 . . 203 206 + MnlI CCTC 213 212 . . 216 220 - MboII GAAGA 208 207 . . 222 225 + AciI CCGC 222 224 . . 222 227 + BsrBI CCGCTC 224 224 . . 232 235 + MseI TTAA 232 234 . . 242 246 - BsrI ACTGG 242 240 . . 256 261 + SduI GDGCHC 260 256 . . 256 261 + HgiAI* GWGCWC 260 256 . . 267 270 + SetI ASST 270 266 . . 267 270 + CviJI RGCY 268 268 . . 267 270 + AluI AGCT 268 268 . . 272 275 + MnlI CCTC 282 281 . . 273 278 + Ksp632I* CTCTTC 279 282 . . 274 278 - MboII GAAGA 266 265 . . 292 302 + MwoI GCNNNNNNNGC 298 295 . . 290 295 - EcoP15I CAGCAG 262 264 . . 301 305 + TauI GCSGC 304 301 . . 301 305 + BlsI GCNGC 303 302 . . 301 305 + BisI GCNGC 302 303 . . 301 304 - AciI CCGC 301 303 . . 307 310 + SetI ASST 310 306 . . 308 312 + Hin4II* CCTTC 318 317 . . 313 323 + BsiYI* CCNNNNNNNGG 319 316 . . 322 325 + CviJI RGCY 323 323 . . 323 333 + MwoI GCNNNNNNNGC 329 326 . . 323 328 + Cac8I GCNNGC 325 325 . . 319 324 - NmeAIII GCCGAG 299 297 . . 326 329 + FnuDII* CGCG 327 327 . . 327 330 - AciI CCGC 327 329 . . 331 334 + SetI ASST 334 330 . . 331 334 + CviJI RGCY 332 332 . . 331 334 + AluI AGCT 332 332 . . 337 340 + MseI TTAA 337 339 . . 346 350 - BsmAI GTCTC 340 344 . . 353 357 + BssKI CCNGG 352 357 . . 353 357 + BseBI CCWGG 354 355 . . 353 357 + ScrFI CCNGG 354 355 . . 353 357 + EcoRII CCWGG 352 357 . . 350 354 - FokI GGATG 336 340 . . 350 354 - BseGI GGATG 349 347 . . 355 360 + BalI TGGCCA 357 357 . . 355 360 + CfrI YGGCCR 355 359 . . 356 359 + CviJI RGCY 357 357 . . 356 359 + HaeIII GGCC 357 357 . . 369 378 + MslI CAYNNNNRTG 373 373 . . 369 372 + FatI CATG 368 372 . . 369 372 + NlaIII CATG 372 368 . . 369 372 + CviAII CATG 369 371 . . 378 383 + SacI GAGCTC 382 378 . . 378 383 + Ecl136II GAGCTC 380 380 . . 378 383 + SduI GDGCHC 382 378 . . 378 383 + HgiJII* GRGCYC 382 378 . . 378 383 + HgiAI* GWGCWC 382 378 . . 379 382 + SetI ASST 382 378 . . 379 382 + CviJI RGCY 380 380 . . 379 382 + AluI AGCT 380 380 . . 383 386 + MnlI CCTC 393 392 . . 381 386 - BseRI GAGGAG 372 370 . . 386 389 + AciI CCGC 386 388 . . 387 390 + FnuDII* CGCG 388 388 . . 395 401 + TspRI CASTGNN 401 392 . . 395 400 - BtsI GCAGTG 394 392 . . 401 406 + TsoI TARCCA 417 415 . . 405 410 + EcoP15I CAGCAG 435 437 . . 407 411 + BlsI GCNGC 409 408 . . 407 411 + BisI GCNGC 408 409 . . 407 411 + BbvI GCAGC 419 423 . . 407 411 + TseI GCWGC 407 410 . . 410 420 + MwoI GCNNNNNNNGC 416 413 . . 410 415 + BsrDI GCAATG 417 415 . . 419 424 + Cac8I GCNNGC 421 421 . . 423 426 + Hin6I GCGC 423 425 . . 423 426 + GlaI GCGC 424 424 . . 423 426 + HhaI GCGC 425 423 . . 424 427 + FnuDII* CGCG 425 425 . . 425 435 + MwoI GCNNNNNNNGC 431 428 . . 425 430 + Cac8I GCNNGC 427 427 . . 427 432 + SduI GDGCHC 431 427 . . 427 437 + BplI GAGNNNNNCTC 418 413 450 445 427 432 + HgiJII* GRGCYC 431 427 . . 428 431 + CviJI RGCY 429 429 . . 430 435 + GsaI CCCAGC 434 430 . . 430 435 + BseYI CCCAGC 430 434 . . 433 436 + SetI ASST 436 432 . . 433 436 + CviJI RGCY 434 434 . . 433 436 + AluI AGCT 434 434 . . 436 440 + Hpy188I TCNGA 438 437 . . 427 437 - BplI GAGNNNNNCTC 450 445 418 413 439 443 + TfiI GAWTC 439 442 . . 439 443 + HinfI GANTC 439 442 . . 448 453 + EcoRV GATATC 450 450 . . 453 461 + AlwNI CAGNNNCTG 458 455 . . 454 457 + SetI ASST 457 453 . . 459 464 + SfeI* CTRYAG 459 463 . . 469 473 + Hpy188I TCNGA 471 470 . . 480 485 + Hpy166II GTNNAC 482 482 . . 484 487 + TaiI ACGT 487 483 . . 484 487 + SetI ASST 487 483 . . 484 487 + MaeII ACGT 484 486 . . 487 491 - TspDTI ATGAA 477 475 . . 494 498 + Hin4II* CCTTC 504 503 . . 501 506 + Cac8I GCNNGC 503 503 . . 504 507 + CviRI* TGCA 505 505 . . 505 510 + BsrDI GCAATG 512 510 . . 570 573 + TspEI AATT 569 573 . . 576 580 - MboII GAAGA 568 567 . . 575 580 - BbvII* GAAGAC 568 572 . . 581 585 + MaeIII GTNAC 580 585 . . 589 595 + TspRI CASTGNN 595 586 . . 590 594 + BsrI ACTGG 595 593 . . 595 600 + PsiI TTATAA 597 597 . . 592 597 - TsoI TARCCA 582 580 . . #--------------------------------------- # # # AciI: # [CCGC] # 6 26 30 59 79 186 # 214 # # AcyI: # [GRCGYC] # 200 400 # # AluI: # [AGCT] # 48 54 62 64 84 122 # 166 # # AlwNI: # [CAGNNNCTG] # 142 458 # # ApoI: # [RAATTY] # 163 437 # # BalI: # [TGGCCA] # 243 357 # # BbvI: # [GCAGC] # 26 155 181 238 # # BbvII*: # [GAAGAC] # 32 568 # # BisI: # [GCNGC] # 51 106 107 144 192 # # BlsI: # [GCNGC] # 51 106 107 145 191 # # BplI: # [GAGNNNNNCTC] # 0 0 32 150 418 # # BseBI: # [CCWGG] # 246 354 # # BseGI: # [GGATG] # 251 349 # # BseRI: # [GAGGAG] # 228 372 # # BseYI: # [CCCAGC] # 170 430 # # BsiYI*: # [CCNNNNNNNGG] # 281 319 # # BsmAI: # [GTCTC] # 13 260 327 # # BsmI: # [GAATGC] # 188 412 # # BsrBI: # [CCGCTC] # 224 376 # # BsrDI: # [GCAATG] # 88 95 417 # # BsrI: # [ACTGG] # 5 242 353 # # BssKI: # [CCNGG] # 248 352 # # BstKTI: # [GATC] # 109 491 # # BtsI: # [GCAGTG] # 206 394 # # Cac8I: # [GCNNGC] # 6 76 96 97 325 # # CfrI: # [YGGCCR] # 245 355 # # CviAII: # [CATG] # 153 216 231 # # CviJI: # [RGCY] # 5 9 23 25 49 55 # 62 84 122 166 # # CviRI*: # [TGCA] # 95 143 362 # # DpnI: # [GATC] # 108 492 # # Ecl136II: # [GAGCTC] # 220 380 # # EcoP15I: # [CAGCAG] # 165 173 262 # # EcoRII: # [CCWGG] # 248 352 # # EcoRV: # [GATATC] # 150 450 # # Esp3I: # [CGTCTC] # 13 587 # # FatI: # [CATG] # 152 216 232 # # FnuDII*: # [CGCG] # 37 61 111 175 216 # # FokI: # [GGATG] # 264 336 # # GlaI: # [GCGC] # 176 424 # # GsaI: # [CCCAGC] # 166 434 # # HaeIII: # [GGCC] # 243 357 # # HgaI: # [GACGC] # 208 392 # # HgiAI*: # [GWGCWC] # 122 218 260 # # HgiJII*: # [GRGCYC] # 49 169 382 # # HhaI: # [GCGC] # 175 425 # # Hin4I: # [GAYNNNNNVTC] # 28 32 540 # # Hin4II*: # [CCTTC] # 96 186 318 # # Hin6I: # [GCGC] # 177 423 # # HinfI: # [GANTC] # 161 439 # # Hpy166II: # [GTNNAC] # 118 482 # # Hpy178III*: # [TCNNGA] # 110 490 # # Hpy188I: # [TCNGA] # 33 129 438 # # Hpy99I: # [CGWCG] # 202 398 # # Ksp632I*: # [CTCTTC] # 279 321 # # MaeII: # [ACGT] # 116 484 # # MaeIII: # [GTNAC] # 20 580 # # MboI: # [GATC] # 106 494 # # MboII: # [GAAGA] # 32 58 208 302 # # McrI*: # [CGRYCG] # 109 491 # # MfeI: # [CAATTG] # 93 507 # # MnlI: # [CCTC] # 69 111 207 213 # # MseI: # [TTAA] # 9 13 27 85 98 105 # 263 # # MslI: # [CAYNNNNRTG] # 227 373 # # MwoI: # [GCNNNNNNNGC] # 15 31 87 169 298 # # NlaIII: # [CATG] # 156 216 228 # # NlaIV: # [GGNNCC] # 190 410 # # NmeAIII: # [GCCGAG] # 299 301 # # NruI: # [TCGCGA] # 111 489 # # NspBII*: # [CMGCKG] # 194 406 # # PsiI: # [TTATAA] # 3 597 # # PstI: # [CTGCAG] # 145 455 # # PvuI: # [CGATCG] # 109 491 # # SacI: # [GAGCTC] # 218 382 # # ScrFI: # [CCNGG] # 246 354 # # SduI: # [GDGCHC] # 49 122 169 260 # # SetI: # [ASST] # 21 24 30 40 48 54 # 64 84 113 122 # # SfeI*: # [CTRYAG] # 141 141 318 # # TaiI: # [ACGT] # 113 487 # # TauI: # [GCSGC] # 296 304 # # TfiI: # [GAWTC] # 161 439 # # TseI: # [GCWGC] # 51 143 193 213 # # TsoI: # [TARCCA] # 18 165 417 # # TspDTI: # [ATGAA] # 123 141 336 # # TspEI: # [AATT] # 27 31 66 70 406 # # TspRI: # [CASTGNN] # 5 194 401 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 600 # Reported_sequences: 1 # Reported_hitcount: 161 #---------------------------------------