Restriction Fragments for YILWsigma1



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

YILWsigma1 on chromosome IX from coordinates 205220 to 205559.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.25667.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 16 27 + BdaI TGANNNNNNTCA 5 3 39 37 16 27 - BdaI TGANNNNNNTCA 39 37 5 3 32 42 + Hin4I GAYNNNNNVTC 23 18 55 50 32 42 + Eam1105I GACNNNNNGTC 37 36 . . 38 41 + TaiI ACGT 41 37 . . 38 41 + SetI ASST 41 37 . . 38 41 + MaeII ACGT 38 40 . . 40 44 + Tsp45I GTSAC 39 44 . . 40 44 + MaeIII GTNAC 39 44 . . 32 42 - Hin4I GAYNNNNNVTC 55 50 23 18 41 45 - HphI GGTGA 33 32 . . 49 54 + AclI AACGTT 50 52 . . 50 53 + TaiI ACGT 53 49 . . 50 53 + SetI ASST 53 49 . . 50 53 + MaeII ACGT 50 52 . . 53 57 - TspDTI ATGAA 43 41 . . 64 69 + NdeI CATATG 65 67 . . 67 71 + TspDTI ATGAA 82 80 . . 75 78 + SetI ASST 78 74 . . 78 83 + PsiI TTATAA 80 80 . . 96 99 + FatI CATG 95 99 . . 96 99 + NlaIII CATG 99 95 . . 96 99 + CviAII CATG 96 98 . . 99 103 - BsmAI GTCTC 93 97 . . 112 115 + SetI ASST 115 111 . . 113 117 + Hin4II* CCTTC 123 122 . . 119 122 + MaeI CTAG 119 121 . . 121 124 + SetI ASST 124 120 . . 121 124 + CviJI RGCY 122 122 . . 121 124 + AluI AGCT 122 122 . . 153 156 + TspEI AATT 152 156 . . 162 167 + XhoII RGATCY 162 166 . . 163 166 + DpnI GATC 164 164 . . 163 166 + BstKTI GATC 165 163 . . 163 166 + MboI GATC 162 166 . . 165 169 + Hpy188I TCNGA 167 166 . . 163 167 - BinI* GGATC 157 158 . . 170 174 + TfiI GAWTC 170 173 . . 170 174 + HinfI GANTC 170 173 . . 174 177 + AciI CCGC 174 176 . . 175 178 + FnuDII* CGCG 176 176 . . 176 179 + Hin6I GCGC 176 178 . . 176 179 + GlaI GCGC 177 177 . . 176 179 + HhaI GCGC 178 176 . . 188 192 + DdeI CTNAG 188 191 . . 196 200 + TfiI GAWTC 196 199 . . 196 200 + HinfI GANTC 196 199 . . 198 202 - TspDTI ATGAA 188 186 . . 226 230 + MaeIII GTNAC 225 230 . . 233 237 - TspGWI ACGGA 223 221 . . 239 242 + TspEI AATT 238 242 . . 240 245 + VspI ATTAAT 241 243 . . 241 244 + MseI TTAA 241 243 . . 245 249 + Hpy188I TCNGA 247 246 . . 253 257 + Tsp4CI* ACNGT 255 254 . . 265 269 + BsrI ACTGG 270 268 . . 269 273 + MaeIII GTNAC 268 273 . . 267 272 - TsoI TARCCA 257 255 . . 278 282 + DdeI CTNAG 278 281 . . 283 287 + Tsp4CI* ACNGT 285 284 . . 302 307 + Hpy178III* TCNNGA 303 305 . . 311 316 + BetI* WCCGGW 311 315 . . 312 315 + HpaII CCGG 312 314 . . 320 327 + PspXI VCTCGAGB 321 325 . . 321 326 + SmlI CTYRAG 321 325 . . 321 326 + XhoI CTCGAG 321 325 . . 321 326 + BmeT110I CYCGRG 322 324 . . 321 326 + AvaI CYCGRG 321 325 . . 322 325 + TaqI TCGA 322 324 . . 324 329 + SduI GDGCHC 328 324 . . 324 329 + HgiJII* GRGCYC 328 324 . . 325 328 + CviJI RGCY 326 326 . . #--------------------------------------- # # # AciI: # [CCGC] # 166 174 # # AclI: # [AACGTT] # 50 290 # # AluI: # [AGCT] # 122 218 # # AvaI: # [CYCGRG] # 19 321 # # BdaI: # [TGANNNNNNTCA] # 0 0 5 34 301 # # BetI*: # [WCCGGW] # 29 311 # # BinI*: # [GGATC] # 157 183 # # BmeT110I: # [CYCGRG] # 18 322 # # BsmAI: # [GTCTC] # 93 247 # # BsrI: # [ACTGG] # 70 270 # # BstKTI: # [GATC] # 165 175 # # CviAII: # [CATG] # 96 244 # # CviJI: # [RGCY] # 14 122 204 # # DdeI: # [CTNAG] # 62 90 188 # # DpnI: # [GATC] # 164 176 # # Eam1105I: # [GACNNNNNGTC] # 37 303 # # FatI: # [CATG] # 95 245 # # FnuDII*: # [CGCG] # 164 176 # # GlaI: # [GCGC] # 163 177 # # HgiJII*: # [GRGCYC] # 12 328 # # HhaI: # [GCGC] # 162 178 # # Hin4I: # [GAYNNNNNVTC] # 0 0 23 32 285 # # Hin4II*: # [CCTTC] # 123 217 # # Hin6I: # [GCGC] # 164 176 # # HinfI: # [GANTC] # 26 144 170 # # HpaII: # [CCGG] # 28 312 # # HphI: # [GGTGA] # 33 307 # # Hpy178III*: # [TCNNGA] # 37 303 # # Hpy188I: # [TCNGA] # 80 93 167 # # MaeI: # [CTAG] # 119 221 # # MaeII: # [ACGT] # 12 38 290 # # MaeIII: # [GTNAC] # 39 43 72 186 # # MboI: # [GATC] # 162 178 # # MseI: # [TTAA] # 99 241 # # NdeI: # [CATATG] # 65 275 # # NlaIII: # [CATG] # 99 241 # # PsiI: # [TTATAA] # 80 260 # # PspXI: # [VCTCGAGB] # 19 321 # # SduI: # [GDGCHC] # 12 328 # # SetI: # [ASST] # 9 12 25 37 41 216 # # SmlI: # [CTYRAG] # 19 321 # # TaiI: # [ACGT] # 12 41 287 # # TaqI: # [TCGA] # 18 322 # # TfiI: # [GAWTC] # 26 144 170 # # TsoI: # [TARCCA] # 83 257 # # Tsp45I: # [GTSAC] # 39 301 # # Tsp4CI*: # [ACNGT] # 30 55 255 # # TspDTI: # [ATGAA] # 39 43 106 152 # # TspEI: # [AATT] # 86 102 152 # # TspGWI: # [ACGGA] # 117 223 # # VspI: # [ATTAAT] # 99 241 # # XhoI: # [CTCGAG] # 19 321 # # XhoII: # [RGATCY] # 162 178 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 340 # Reported_sequences: 1 # Reported_hitcount: 72 #---------------------------------------