Restriction Fragments for YILCdelta5



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The currently selected gene/sequence is :

YILCdelta5 on chromosome IX from coordinates 336897 to 336708.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.12341.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 2 7 + AhaIII* TTTAAA 4 4 . . 3 6 + MseI TTAA 3 5 . . 8 12 + TspDTI ATGAA 23 21 . . 12 17 + ApoI RAATTY 12 16 . . 13 16 + TspEI AATT 12 16 . . 21 26 + SnaBI TACGTA 23 23 . . 21 26 + BsaAI YACGTR 23 23 . . 22 25 + TaiI ACGT 25 21 . . 22 25 + SetI ASST 25 21 . . 22 25 + MaeII ACGT 22 24 . . 29 32 + TaqI TCGA 29 31 . . 32 37 + SpeI ACTAGT 32 36 . . 33 36 + MaeI CTAG 33 35 . . 39 43 - TspDTI ATGAA 29 27 . . 48 51 + MaeI CTAG 48 50 . . 51 56 + NspI RCATGY 55 51 . . 52 55 + FatI CATG 51 55 . . 52 55 + NlaIII CATG 55 51 . . 52 55 + CviAII CATG 52 54 . . 55 59 + MaeIII GTNAC 54 59 . . 97 100 + Csp6I GTAC 97 99 . . 97 100 + RsaI GTAC 98 98 . . 101 105 + BccI CCATC 109 110 . . 104 108 + Hpy188I TCNGA 106 105 . . 110 113 + MseI TTAA 110 112 . . 113 117 + TspDTI ATGAA 128 126 . . 115 120 + BsmI GAATGC 121 119 . . 126 129 + CviRI* TGCA 127 127 . . 152 156 + TspDTI ATGAA 167 165 . . 176 179 + TspEI AATT 175 179 . . 182 187 + SspI AATATT 184 184 . . #--------------------------------------- # # # AhaIII*: # [TTTAAA] # 4 186 # # ApoI: # [RAATTY] # 12 178 # # BccI: # [CCATC] # 81 109 # # BsaAI: # [YACGTR] # 23 167 # # BsmI: # [GAATGC] # 69 121 # # Csp6I: # [GTAC] # 93 97 # # CviAII: # [CATG] # 52 138 # # CviRI*: # [TGCA] # 63 127 # # FatI: # [CATG] # 51 139 # # Hpy188I: # [TCNGA] # 84 106 # # MaeI: # [CTAG] # 15 33 142 # # MaeII: # [ACGT] # 22 168 # # MaeIII: # [GTNAC] # 54 136 # # MseI: # [TTAA] # 3 80 107 # # NlaIII: # [CATG] # 55 135 # # NspI: # [RCATGY] # 55 135 # # RsaI: # [GTAC] # 92 98 # # SetI: # [ASST] # 25 165 # # SnaBI: # [TACGTA] # 23 167 # # SpeI: # [ACTAGT] # 32 158 # # SspI: # [AATATT] # 6 184 # # TaiI: # [ACGT] # 25 165 # # TaqI: # [TCGA] # 29 161 # # TspDTI: # [ATGAA] # 6 23 23 39 99 # # TspEI: # [AATT] # 12 15 163 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 190 # Reported_sequences: 1 # Reported_hitcount: 31 #---------------------------------------