Restriction Fragments for CSS1/YIL169C



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The currently selected gene/sequence is :

CSS1/YIL169C on chromosome IX from coordinates 26106 to 23119.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.25702.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 11 18 + PmeI GTTTAAAC 14 14 . . 12 17 + AhaIII* TTTAAA 14 14 . . 13 16 + MseI TTAA 13 15 . . 19 24 + ApoI RAATTY 19 23 . . 20 23 + TspEI AATT 19 23 . . 27 30 + SetI ASST 30 26 . . 27 30 + CviJI RGCY 28 28 . . 27 30 + AluI AGCT 28 28 . . 28 38 + MwoI GCNNNNNNNGC 34 31 . . 28 32 + BlsI GCNGC 30 29 . . 28 32 + BisI GCNGC 29 30 . . 28 32 + TseI GCWGC 28 31 . . 28 32 - BbvI GCAGC 15 19 . . 36 39 + SetI ASST 39 35 . . 36 39 + CviJI RGCY 37 37 . . 36 39 + AluI AGCT 37 37 . . 42 45 + CviJI RGCY 43 43 . . 42 45 + HaeIII GGCC 43 43 . . 42 46 + BmgT120I GGNCC 43 44 . . 42 46 + AsuI* GGNCC 42 45 . . 60 63 + CviRI* TGCA 61 61 . . 71 76 + TatI WGTACW 71 75 . . 71 76 + ScaI AGTACT 73 73 . . 72 75 + Csp6I GTAC 72 74 . . 72 75 + RsaI GTAC 73 73 . . 75 80 + SfeI* CTRYAG 75 79 . . 88 91 + SetI ASST 91 87 . . 89 92 + MnlI CCTC 99 98 . . 93 96 + TspEI AATT 92 96 . . 106 109 + SetI ASST 109 105 . . 106 109 + CviJI RGCY 107 107 . . 106 109 + AluI AGCT 107 107 . . 113 124 + BstXI CCANNNNNNTGG 120 116 . . 114 123 + MslI CAYNNNNRTG 118 118 . . 110 114 - FokI GGATG 96 100 . . 110 114 - BseGI GGATG 109 107 . . 114 123 + OliI CACNNNNGTG 118 118 . . 115 118 + SetI ASST 118 114 . . 116 119 + MnlI CCTC 126 125 . . 134 137 + MnlI CCTC 144 143 . . 136 141 + Hpy178III* TCNNGA 137 139 . . 139 144 + XhoII RGATCY 139 143 . . 139 144 + NlaIV GGNNCC 141 141 . . 139 144 + BamHI GGATCC 139 143 . . 139 143 + BinI* GGATC 147 148 . . 140 143 + DpnI GATC 141 141 . . 140 143 + BstKTI GATC 142 140 . . 140 143 + MboI GATC 139 143 . . 140 144 - BinI* GGATC 134 135 . . 142 146 - TspGWI ACGGA 132 130 . . 148 151 + TaqI TCGA 148 150 . . 150 153 + DpnI GATC 151 151 . . 150 153 + BstKTI GATC 152 150 . . 150 153 + MboI GATC 149 153 . . 167 171 + DdeI CTNAG 167 170 . . 169 179 + BplI GAGNNNNNCTC 160 155 192 187 167 171 - BspCNI CTCAG 159 157 . . 167 171 - BseMII CTCAG 158 156 . . 171 182 + DrdI GACNNNNNNGTC 177 175 . . 169 173 - BsmAI GTCTC 163 167 . . 176 179 + MnlI CCTC 186 185 . . 173 177 - FokI GGATG 159 163 . . 173 177 - BseGI GGATG 172 170 . . 169 179 - BplI GAGNNNNNCTC 192 187 160 155 182 185 + AciI CCGC 182 184 . . 190 195 + EcoRV GATATC 192 192 . . 195 200 + SmlI CTYRAG 195 199 . . 195 200 + AflII CTTAAG 195 199 . . 196 199 + MseI TTAA 196 198 . . 216 219 + SetI ASST 219 215 . . 216 219 + CviJI RGCY 217 217 . . 216 219 + AluI AGCT 217 217 . . 219 223 - TspDTI ATGAA 209 207 . . 221 225 - FokI GGATG 207 211 . . 221 225 - BseGI GGATG 220 218 . . 235 239 + BsmAI GTCTC 240 244 . . 236 241 + Hpy178III* TCNNGA 237 239 . . 237 242 + SmlI CTYRAG 237 241 . . 237 242 + XhoI CTCGAG 237 241 . . 237 242 + BmeT110I CYCGRG 238 240 . . 237 242 + AvaI CYCGRG 237 241 . . 238 241 + TaqI TCGA 238 240 . . 240 245 + SacI GAGCTC 244 240 . . 240 245 + Ecl136II GAGCTC 242 242 . . 240 245 + SduI GDGCHC 244 240 . . 240 245 + HgiJII* GRGCYC 244 240 . . 240 245 + HgiAI* GWGCWC 244 240 . . 241 244 + SetI ASST 244 240 . . 241 244 + CviJI RGCY 242 242 . . 241 244 + AluI AGCT 242 242 . . 250 254 + BmgT120I GGNCC 251 252 . . 250 254 + AvaII GGWCC 250 253 . . 250 254 + AsuI* GGNCC 250 253 . . 248 253 - TaqII GACCGA 238 236 . . 253 257 + BccI CCATC 261 262 . . 257 260 + MnlI CCTC 267 266 . . 254 258 - FokI GGATG 240 244 . . 258 263 + Ksp632I* CTCTTC 264 267 . . 254 258 - BseGI GGATG 253 251 . . 263 266 + MnlI CCTC 273 272 . . 259 263 - MboII GAAGA 251 250 . . 271 275 + BsmAI GTCTC 276 280 . . 273 278 + SmlI CTYRAG 273 277 . . 277 280 + SetI ASST 280 276 . . 277 280 + CviJI RGCY 278 278 . . 277 280 + AluI AGCT 278 278 . . 278 288 + MwoI GCNNNNNNNGC 284 281 . . 273 278 - Bce83I* CTTGAG 258 256 . . 286 289 + CviJI RGCY 287 287 . . 293 296 + MnlI CCTC 303 302 . . 294 299 + Ksp632I* CTCTTC 300 303 . . 295 299 - MboII GAAGA 287 286 . . 297 301 - TspDTI ATGAA 287 285 . . 299 303 - FokI GGATG 285 289 . . 299 303 - BseGI GGATG 298 296 . . 301 305 - TspGWI ACGGA 291 289 . . 307 311 + Hpy188I TCNGA 309 308 . . 319 322 + SetI ASST 322 318 . . 319 322 + CviJI RGCY 320 320 . . 319 322 + AluI AGCT 320 320 . . 334 338 - MboII GAAGA 326 325 . . 337 341 - MboII GAAGA 329 328 . . 340 344 - MboII GAAGA 332 331 . . 344 352 + AlwNI CAGNNNCTG 349 346 . . 345 348 + SetI ASST 348 344 . . 345 348 + CviJI RGCY 346 346 . . 345 348 + AluI AGCT 346 346 . . 341 346 - Eco57MI CTGRAG 326 324 . . 341 346 - Eco57I CTGAAG 326 324 . . 349 353 + Hpy188I TCNGA 351 350 . . 355 359 + BsmAI GTCTC 360 364 . . 357 362 + SmlI CTYRAG 357 361 . . 361 364 + SetI ASST 364 360 . . 361 364 + CviJI RGCY 362 362 . . 361 364 + AluI AGCT 362 362 . . 357 362 - Bce83I* CTTGAG 342 340 . . 378 381 + SetI ASST 381 377 . . 378 381 + CviJI RGCY 379 379 . . 378 381 + AluI AGCT 379 379 . . 383 386 + MnlI CCTC 393 392 . . 391 395 + Hpy188I TCNGA 393 392 . . 397 401 + BsmAI GTCTC 402 406 . . 399 404 + SmlI CTYRAG 399 403 . . 403 406 + SetI ASST 406 402 . . 403 406 + CviJI RGCY 404 404 . . 403 406 + AluI AGCT 404 404 . . 399 404 - Bce83I* CTTGAG 384 382 . . 418 422 - MboII GAAGA 410 409 . . 421 425 - MboII GAAGA 413 412 . . 424 428 - MboII GAAGA 416 415 . . 428 436 + AlwNI CAGNNNCTG 433 430 . . 429 432 + SetI ASST 432 428 . . 429 432 + CviJI RGCY 430 430 . . 429 432 + AluI AGCT 430 430 . . 425 430 - Eco57MI CTGRAG 410 408 . . 425 430 - Eco57I CTGAAG 410 408 . . 433 437 + Hpy188I TCNGA 435 434 . . 445 448 + SetI ASST 448 444 . . 445 448 + CviJI RGCY 446 446 . . 445 448 + AluI AGCT 446 446 . . 460 464 - MboII GAAGA 452 451 . . 467 470 + MnlI CCTC 477 476 . . 463 467 - MboII GAAGA 455 454 . . 467 473 + Bpu10I CCTNAGC 468 471 . . 467 473 + BbvCI CCTCAGC 468 471 . . 468 472 + DdeI CTNAG 468 471 . . 468 472 + BspCNI CTCAG 481 479 . . 468 472 + BseMII CTCAG 482 480 . . 470 478 + AlwNI CAGNNNCTG 475 472 . . 471 474 + SetI ASST 474 470 . . 471 474 + CviJI RGCY 472 472 . . 471 474 + AluI AGCT 472 472 . . 475 479 + Hpy188I TCNGA 477 476 . . 481 485 + BsmAI GTCTC 486 490 . . 483 488 + SmlI CTYRAG 483 487 . . 487 490 + SetI ASST 490 486 . . 487 490 + CviJI RGCY 488 488 . . 487 490 + AluI AGCT 488 488 . . 483 488 - Bce83I* CTTGAG 468 466 . . 504 507 + SetI ASST 507 503 . . 504 507 + CviJI RGCY 505 505 . . 504 507 + AluI AGCT 505 505 . . 505 515 + MwoI GCNNNNNNNGC 511 508 . . 509 512 + MnlI CCTC 519 518 . . 509 515 + Bpu10I CCTNAGC 510 513 . . 509 515 + BbvCI CCTCAGC 510 513 . . 510 514 + DdeI CTNAG 510 513 . . 510 514 + BspCNI CTCAG 523 521 . . 510 514 + BseMII CTCAG 524 522 . . 512 520 + AlwNI CAGNNNCTG 517 514 . . 513 516 + SetI ASST 516 512 . . 513 516 + CviJI RGCY 514 514 . . 513 516 + AluI AGCT 514 514 . . 517 521 + Hpy188I TCNGA 519 518 . . 523 527 + BsmAI GTCTC 528 532 . . 525 530 + SmlI CTYRAG 525 529 . . 529 532 + SetI ASST 532 528 . . 529 532 + CviJI RGCY 530 530 . . 529 532 + AluI AGCT 530 530 . . 525 530 - Bce83I* CTTGAG 510 508 . . 546 549 + SetI ASST 549 545 . . 546 549 + CviJI RGCY 547 547 . . 546 549 + AluI AGCT 547 547 . . 551 554 + MnlI CCTC 561 560 . . 559 563 + Hpy188I TCNGA 561 560 . . 565 569 + BsmAI GTCTC 570 574 . . 567 572 + SmlI CTYRAG 567 571 . . 571 574 + SetI ASST 574 570 . . 571 574 + CviJI RGCY 572 572 . . 571 574 + AluI AGCT 572 572 . . 567 572 - Bce83I* CTTGAG 552 550 . . 586 590 - MboII GAAGA 578 577 . . 589 593 - MboII GAAGA 581 580 . . 592 596 - MboII GAAGA 584 583 . . 596 604 + AlwNI CAGNNNCTG 601 598 . . 597 600 + SetI ASST 600 596 . . 597 600 + CviJI RGCY 598 598 . . 597 600 + AluI AGCT 598 598 . . 593 598 - Eco57MI CTGRAG 578 576 . . 593 598 - Eco57I CTGAAG 578 576 . . 601 605 + Hpy188I TCNGA 603 602 . . 613 616 + SetI ASST 616 612 . . 613 616 + CviJI RGCY 614 614 . . 613 616 + AluI AGCT 614 614 . . 628 632 - MboII GAAGA 620 619 . . 635 638 + MnlI CCTC 645 644 . . 631 635 - MboII GAAGA 623 622 . . 635 641 + Bpu10I CCTNAGC 636 639 . . 635 641 + BbvCI CCTCAGC 636 639 . . 636 640 + DdeI CTNAG 636 639 . . 636 640 + BspCNI CTCAG 649 647 . . 636 640 + BseMII CTCAG 650 648 . . 638 646 + AlwNI CAGNNNCTG 643 640 . . 639 642 + SetI ASST 642 638 . . 639 642 + CviJI RGCY 640 640 . . 639 642 + AluI AGCT 640 640 . . 643 647 + Hpy188I TCNGA 645 644 . . 649 653 + BsmAI GTCTC 654 658 . . 651 656 + SmlI CTYRAG 651 655 . . 655 658 + SetI ASST 658 654 . . 655 658 + CviJI RGCY 656 656 . . 655 658 + AluI AGCT 656 656 . . 651 656 - Bce83I* CTTGAG 636 634 . . 672 675 + SetI ASST 675 671 . . 672 675 + CviJI RGCY 673 673 . . 672 675 + AluI AGCT 673 673 . . 677 680 + MnlI CCTC 687 686 . . 685 689 + Hpy188I TCNGA 687 686 . . 691 695 + BsmAI GTCTC 696 700 . . 693 698 + SmlI CTYRAG 693 697 . . 697 700 + SetI ASST 700 696 . . 697 700 + CviJI RGCY 698 698 . . 697 700 + AluI AGCT 698 698 . . 693 698 - Bce83I* CTTGAG 678 676 . . 714 717 + SetI ASST 717 713 . . 714 717 + CviJI RGCY 715 715 . . 714 717 + AluI AGCT 715 715 . . 719 722 + MnlI CCTC 729 728 . . 733 737 + BsmAI GTCTC 738 742 . . 735 740 + SmlI CTYRAG 735 739 . . 739 742 + SetI ASST 742 738 . . 739 742 + CviJI RGCY 740 740 . . 739 742 + AluI AGCT 740 740 . . 740 750 + MwoI GCNNNNNNNGC 746 743 . . 735 740 - Bce83I* CTTGAG 720 718 . . 746 750 + BlsI GCNGC 748 747 . . 746 750 + BisI GCNGC 747 748 . . 746 750 + BbvI GCAGC 758 762 . . 746 750 + TseI GCWGC 746 749 . . 748 751 + CviJI RGCY 749 749 . . 757 761 + BsmAI GTCTC 762 766 . . 764 767 + AciI CCGC 764 766 . . 761 765 - FokI GGATG 747 751 . . 761 765 - BseGI GGATG 760 758 . . 775 778 + SetI ASST 778 774 . . 775 778 + CviJI RGCY 776 776 . . 775 778 + AluI AGCT 776 776 . . 777 782 + SmlI CTYRAG 777 781 . . 781 784 + SetI ASST 784 780 . . 781 784 + CviJI RGCY 782 782 . . 781 784 + AluI AGCT 782 782 . . 777 782 - Bce83I* CTTGAG 762 760 . . 790 793 + MnlI CCTC 800 799 . . 799 802 + SetI ASST 802 798 . . 800 803 + MnlI CCTC 810 809 . . 806 809 + AciI CCGC 806 808 . . 803 807 - FokI GGATG 789 793 . . 803 807 - BseGI GGATG 802 800 . . 808 818 + MwoI GCNNNNNNNGC 814 811 . . 818 821 + MnlI CCTC 828 827 . . 820 825 + BetI* WCCGGW 820 824 . . 821 824 + HpaII CCGG 821 823 . . 838 841 + TspEI AATT 837 841 . . 843 848 + SmlI CTYRAG 843 847 . . 843 848 + Bce83I* CTTGAG 864 862 . . 859 863 - MboII GAAGA 851 850 . . 865 869 + SfaNI GCATC 874 878 . . 860 865 - Eco57MI CTGRAG 845 843 . . 865 875 + MwoI GCNNNNNNNGC 871 868 . . 865 875 + BstAPI GCANNNNNTGC 871 868 . . 860 865 - Eco57I CTGAAG 845 843 . . 869 872 + MaeI CTAG 869 871 . . 873 876 + CviRI* TGCA 874 874 . . 874 879 + Cac8I GCNNGC 876 876 . . 878 881 + Hin6I GCGC 878 880 . . 878 881 + GlaI GCGC 879 879 . . 878 881 + HhaI GCGC 880 878 . . 889 894 + MmeI TCCRAC 914 912 . . 901 905 - MboII GAAGA 893 892 . . 908 913 + BtgZI GCGATG 923 927 . . 914 917 + Csp6I GTAC 914 916 . . 910 914 - BccI CCATC 904 905 . . 914 917 + RsaI GTAC 915 915 . . 942 948 + TspRI CASTGNN 948 939 . . 946 950 + HphI GGTGA 958 957 . . 949 952 + DpnI GATC 950 950 . . 949 952 + BstKTI GATC 951 949 . . 949 952 + MboI GATC 948 952 . . 961 965 + BsrI ACTGG 966 964 . . 972 975 + TspEI AATT 971 975 . . 975 978 + CviRI* TGCA 976 976 . . 984 987 + SetI ASST 987 983 . . 982 986 - Hin4II* CCTTC 976 975 . . 997 1007 + MwoI GCNNNNNNNGC 1003 1000 . . 997 1007 + BstAPI GCANNNNNTGC 1003 1000 . . 997 1001 + BlsI GCNGC 999 998 . . 997 1001 + BisI GCNGC 998 999 . . 997 1001 + BbvI GCAGC 1009 1013 . . 997 1001 + TseI GCWGC 997 1000 . . 998 1003 + PvuII CAGCTG 1000 1000 . . 998 1003 + NspBII* CMGCKG 1000 1000 . . 999 1002 + SetI ASST 1002 998 . . 999 1002 + CviJI RGCY 1000 1000 . . 999 1002 + AluI AGCT 1000 1000 . . 1003 1008 + NlaIV GGNNCC 1005 1005 . . 1003 1008 + HgiCI* GGYRCC 1003 1007 . . 1010 1013 + TspEI AATT 1009 1013 . . 1014 1018 + HinfI GANTC 1014 1017 . . 1014 1018 - PleI GAGTC 1008 1009 . . 1014 1018 - MlyI GAGTC 1008 1008 . . 1025 1029 + Tsp4CI* ACNGT 1027 1026 . . 1027 1031 + HphI GGTGA 1039 1038 . . 1028 1032 + Tsp45I GTSAC 1027 1032 . . 1028 1032 + MaeIII GTNAC 1027 1032 . . 1034 1039 + SspI AATATT 1036 1036 . . 1044 1048 + DdeI CTNAG 1044 1047 . . 1044 1048 + BspCNI CTCAG 1057 1055 . . 1044 1048 + BseMII CTCAG 1058 1056 . . 1046 1051 + PvuII CAGCTG 1048 1048 . . 1046 1051 + NspBII* CMGCKG 1048 1048 . . 1047 1050 + SetI ASST 1050 1046 . . 1047 1050 + CviJI RGCY 1048 1048 . . 1047 1050 + AluI AGCT 1048 1048 . . 1051 1062 + DrdI GACNNNNNNGTC 1057 1055 . . 1052 1055 + SetI ASST 1055 1051 . . 1060 1065 + Hpy166II GTNNAC 1062 1062 . . 1060 1065 + AccI GTMKAC 1061 1063 . . 1064 1068 + Tsp4CI* ACNGT 1066 1065 . . 1066 1070 + HphI GGTGA 1078 1077 . . 1067 1071 + Tsp45I GTSAC 1066 1071 . . 1067 1071 + MaeIII GTNAC 1066 1071 . . 1096 1099 + SetI ASST 1099 1095 . . 1102 1106 + BssKI CCNGG 1101 1106 . . 1102 1106 + BseBI CCWGG 1103 1104 . . 1102 1106 + ScrFI CCNGG 1103 1104 . . 1102 1106 + EcoRII CCWGG 1101 1106 . . 1104 1107 + SetI ASST 1107 1103 . . 1105 1110 + KpnI GGTACC 1109 1105 . . 1105 1110 + NlaIV GGNNCC 1107 1107 . . 1105 1110 + HgiCI* GGYRCC 1105 1109 . . 1105 1110 + Acc65I GGTACC 1105 1109 . . 1106 1109 + Csp6I GTAC 1106 1108 . . 1106 1109 + RsaI GTAC 1107 1107 . . 1111 1116 + EcoRI GAATTC 1111 1115 . . 1111 1116 + ApoI RAATTY 1111 1115 . . 1112 1115 + TspEI AATT 1111 1115 . . 1115 1118 + TaqI TCGA 1115 1117 . . 1123 1128 + BetI* WCCGGW 1123 1127 . . 1124 1127 + HpaII CCGG 1124 1126 . . 1126 1130 + HphI GGTGA 1138 1137 . . 1139 1142 + TaiI ACGT 1142 1138 . . 1139 1142 + SetI ASST 1142 1138 . . 1139 1142 + MaeII ACGT 1139 1141 . . 1141 1145 + Tsp45I GTSAC 1140 1145 . . 1141 1145 + MaeIII GTNAC 1140 1145 . . 1138 1143 - BtrI CACGTC 1140 1140 . . 1144 1149 + Cfr10I RCCGGY 1144 1148 . . 1144 1149 + BetI* WCCGGW 1144 1148 . . 1144 1149 + AgeI ACCGGT 1144 1148 . . 1145 1148 + HpaII CCGG 1145 1147 . . 1148 1152 + MaeIII GTNAC 1147 1152 . . 1160 1165 + Eco57MI CTGRAG 1181 1179 . . 1160 1165 + Eco57I CTGAAG 1181 1179 . . 1162 1166 + MboII GAAGA 1174 1173 . . 1165 1169 + TfiI GAWTC 1165 1168 . . 1165 1169 + HinfI GANTC 1165 1168 . . 1169 1174 + NspBII* CMGCKG 1171 1171 . . 1169 1172 + AciI CCGC 1169 1171 . . 1171 1175 + BlsI GCNGC 1173 1172 . . 1171 1175 + BisI GCNGC 1172 1173 . . 1171 1175 + TseI GCWGC 1171 1174 . . 1174 1184 + MwoI GCNNNNNNNGC 1180 1177 . . 1171 1175 - BbvI GCAGC 1158 1162 . . 1177 1180 + SetI ASST 1180 1176 . . 1178 1181 + MnlI CCTC 1188 1187 . . 1182 1185 + CviRI* TGCA 1183 1183 . . 1183 1187 + SfaNI GCATC 1192 1196 . . 1187 1191 + BccI CCATC 1195 1196 . . 1184 1188 - FokI GGATG 1170 1174 . . 1184 1188 - BseGI GGATG 1183 1181 . . 1196 1200 + Hin4II* CCTTC 1206 1205 . . 1220 1224 + Tsp4CI* ACNGT 1222 1221 . . 1221 1227 + TspRI CASTGNN 1227 1218 . . 1225 1229 + HphI GGTGA 1237 1236 . . 1226 1230 + Tsp45I GTSAC 1225 1230 . . 1226 1230 + MaeIII GTNAC 1225 1230 . . 1236 1241 + SmlI CTYRAG 1236 1240 . . 1236 1241 + AflII CTTAAG 1236 1240 . . 1237 1240 + MseI TTAA 1237 1239 . . 1257 1261 - Hin4II* CCTTC 1251 1250 . . 1261 1265 + HphI GGTGA 1273 1272 . . 1267 1271 + Tsp45I GTSAC 1266 1271 . . 1267 1271 + MaeIII GTNAC 1266 1271 . . 1269 1280 + TstI CACNNNNNNTCC 1260 1255 1292 1287 1270 1280 + BsaXI ACNNNNNCTCC 1260 1257 1290 1287 1288 1291 + TspEI AATT 1287 1291 . . 1294 1299 + NlaIV GGNNCC 1296 1296 . . 1294 1299 + HgiCI* GGYRCC 1294 1298 . . 1298 1302 + Hin4II* CCTTC 1308 1307 . . 1307 1312 + Hpy178III* TCNNGA 1308 1310 . . 1309 1312 + TaqI TCGA 1309 1311 . . 1325 1329 + Tsp4CI* ACNGT 1327 1326 . . 1339 1344 + BetI* WCCGGW 1339 1343 . . 1340 1343 + HpaII CCGG 1340 1342 . . 1343 1348 + Hpy166II GTNNAC 1345 1345 . . 1343 1348 + AccI GTMKAC 1344 1346 . . 1350 1353 + TaiI ACGT 1353 1349 . . 1350 1353 + SetI ASST 1353 1349 . . 1350 1353 + MaeII ACGT 1350 1352 . . 1355 1359 - BsmAI GTCTC 1349 1353 . . 1355 1360 - Esp3I CGTCTC 1349 1353 . . 1362 1368 + TspRI CASTGNN 1368 1359 . . 1364 1369 + Eco57MI CTGRAG 1385 1383 . . 1364 1369 + Eco57I CTGAAG 1385 1383 . . 1368 1371 + SetI ASST 1371 1367 . . 1366 1370 - Hin4II* CCTTC 1360 1359 . . 1375 1380 + Hpy166II GTNNAC 1377 1377 . . 1382 1386 + Tsp4CI* ACNGT 1384 1383 . . 1384 1388 + HphI GGTGA 1396 1395 . . 1389 1392 + TspEI AATT 1388 1392 . . 1391 1394 + MseI TTAA 1391 1393 . . 1394 1397 + SetI ASST 1397 1393 . . 1396 1399 + MaeI CTAG 1396 1398 . . 1409 1412 + Csp6I GTAC 1409 1411 . . 1409 1412 + RsaI GTAC 1410 1410 . . 1411 1414 + SetI ASST 1414 1410 . . 1424 1427 + TaqI TCGA 1424 1426 . . 1429 1439 + BsiYI* CCNNNNNNNGG 1435 1432 . . 1429 1439 + PflMI CCANNNNNTGG 1435 1432 . . 1430 1438 + AlwNI CAGNNNCTG 1435 1432 . . 1429 1433 - BsrI ACTGG 1429 1427 . . 1435 1440 + Hpy178III* TCNNGA 1436 1438 . . 1443 1446 + SetI ASST 1446 1442 . . 1445 1448 + Csp6I GTAC 1445 1447 . . 1445 1448 + RsaI GTAC 1446 1446 . . 1447 1451 + Tsp4CI* ACNGT 1449 1448 . . 1450 1455 + Hpy166II GTNNAC 1452 1452 . . 1450 1455 + HindII GTYRAC 1452 1452 . . 1462 1467 + BetI* WCCGGW 1462 1466 . . 1463 1466 + HpaII CCGG 1463 1465 . . 1465 1471 + BstEII GGTNACC 1465 1470 . . 1466 1470 + MaeIII GTNAC 1465 1470 . . 1469 1472 + SetI ASST 1472 1468 . . 1498 1502 + BsrI ACTGG 1503 1501 . . 1500 1505 + BalI TGGCCA 1502 1502 . . 1500 1505 + CfrI YGGCCR 1500 1504 . . 1501 1504 + CviJI RGCY 1502 1502 . . 1501 1504 + HaeIII GGCC 1502 1502 . . 1507 1511 + Tsp4CI* ACNGT 1509 1508 . . 1522 1526 + HphI GGTGA 1534 1533 . . 1526 1536 + FalI AAGNNNNNCTT 1517 1512 1549 1544 1527 1530 + SetI ASST 1530 1526 . . 1525 1529 - Hin4II* CCTTC 1519 1518 . . 1526 1536 - FalI AAGNNNNNCTT 1549 1544 1517 1512 1540 1545 + Hpy166II GTNNAC 1542 1542 . . 1540 1545 + HindII GTYRAC 1542 1542 . . 1551 1562 + BcgI CGANNNNNNTGC 1540 1538 1574 1572 1563 1574 + TstI CACNNNNNNTCC 1554 1549 1586 1581 1573 1583 + BsiYI* CCNNNNNNNGG 1579 1576 . . 1582 1585 + CviJI RGCY 1583 1583 . . 1581 1586 - TsoI TARCCA 1571 1569 . . 1587 1593 + TspRI CASTGNN 1593 1584 . . 1588 1592 + BsrI ACTGG 1593 1591 . . 1602 1605 + TspEI AATT 1601 1605 . . 1606 1616 + MwoI GCNNNNNNNGC 1612 1609 . . 1613 1618 + NspBII* CMGCKG 1615 1615 . . 1613 1616 + AciI CCGC 1613 1615 . . 1623 1629 + TspRI CASTGNN 1629 1620 . . 1623 1628 - BtsI GCAGTG 1622 1620 . . 1640 1644 + Tsp4CI* ACNGT 1642 1641 . . 1643 1646 + Csp6I GTAC 1643 1645 . . 1643 1646 + RsaI GTAC 1644 1644 . . 1648 1653 + Cfr10I RCCGGY 1648 1652 . . 1648 1653 + BetI* WCCGGW 1648 1652 . . 1648 1653 + AgeI ACCGGT 1648 1652 . . 1649 1652 + HpaII CCGG 1649 1651 . . 1662 1665 + CviRI* TGCA 1663 1663 . . 1668 1673 + StyI CCWWGG 1668 1672 . . 1668 1673 + SecI* CCNNGG 1668 1672 . . 1671 1674 + SetI ASST 1674 1670 . . 1673 1677 + MaeIII GTNAC 1672 1677 . . 1682 1685 + TspEI AATT 1681 1685 . . 1699 1704 + KpnI GGTACC 1703 1699 . . 1699 1704 + NlaIV GGNNCC 1701 1701 . . 1699 1704 + HgiCI* GGYRCC 1699 1703 . . 1699 1704 + Acc65I GGTACC 1699 1703 . . 1700 1703 + Csp6I GTAC 1700 1702 . . 1700 1703 + RsaI GTAC 1701 1701 . . 1702 1707 + BetI* WCCGGW 1702 1706 . . 1703 1706 + HpaII CCGG 1703 1705 . . 1706 1710 + TfiI GAWTC 1706 1709 . . 1706 1710 + HinfI GANTC 1706 1709 . . 1714 1717 + SetI ASST 1717 1713 . . 1714 1717 + CviJI RGCY 1715 1715 . . 1714 1717 + AluI AGCT 1715 1715 . . 1710 1715 - Eco57MI CTGRAG 1695 1693 . . 1710 1715 - GsuI CTGGAG 1695 1693 . . 1717 1721 + Hpy188I TCNGA 1719 1718 . . 1720 1731 + DrdI GACNNNNNNGTC 1726 1724 . . 1722 1727 - Eco57MI CTGRAG 1707 1705 . . 1722 1727 - Eco57I CTGAAG 1707 1705 . . 1728 1733 + Esp3I CGTCTC 1734 1738 . . 1729 1733 + BsmAI GTCTC 1734 1738 . . 1727 1731 - HgaI GACGC 1716 1721 . . 1732 1736 + Hpy188I TCNGA 1734 1733 . . 1733 1738 + Eco57MI CTGRAG 1754 1752 . . 1733 1738 + Eco57I CTGAAG 1754 1752 . . 1739 1743 + TfiI GAWTC 1739 1742 . . 1735 1739 - Hin4II* CCTTC 1729 1728 . . 1739 1743 + HinfI GANTC 1739 1742 . . 1746 1749 + CviRI* TGCA 1747 1747 . . 1749 1752 + SetI ASST 1752 1748 . . 1747 1752 - BspMI ACCTGC 1738 1742 . . 1747 1753 - AarI CACCTGC 1738 1742 . . 1766 1769 + SetI ASST 1769 1765 . . 1789 1799 + MwoI GCNNNNNNNGC 1795 1792 . . 1792 1797 + SpeI ACTAGT 1792 1796 . . 1793 1796 + MaeI CTAG 1793 1795 . . 1798 1802 + TauI GCSGC 1801 1798 . . 1798 1802 + BlsI GCNGC 1800 1799 . . 1798 1802 + BisI GCNGC 1799 1800 . . 1799 1802 + AciI CCGC 1799 1801 . . 1804 1808 - MboII GAAGA 1796 1795 . . 1806 1810 - TspDTI ATGAA 1796 1794 . . 1812 1818 + TspRI CASTGNN 1818 1809 . . 1808 1812 - FokI GGATG 1794 1798 . . 1808 1812 - BseGI GGATG 1807 1805 . . 1815 1818 + CviRI* TGCA 1816 1816 . . 1816 1820 + SfaNI GCATC 1825 1829 . . 1816 1826 + MwoI GCNNNNNNNGC 1822 1819 . . 1816 1826 + BstAPI GCANNNNNTGC 1822 1819 . . 1812 1817 - BtsI GCAGTG 1811 1809 . . 1829 1832 + AciI CCGC 1829 1831 . . 1837 1841 + MaeIII GTNAC 1836 1841 . . 1834 1838 - TspGWI ACGGA 1824 1822 . . 1840 1845 + Cfr10I RCCGGY 1840 1844 . . 1840 1845 + BetI* WCCGGW 1840 1844 . . 1840 1845 + AgeI ACCGGT 1840 1844 . . 1841 1844 + HpaII CCGG 1841 1843 . . 1851 1855 - TspDTI ATGAA 1841 1839 . . 1867 1871 + MaeIII GTNAC 1866 1871 . . 1864 1868 - TspGWI ACGGA 1854 1852 . . 1870 1875 + Cfr10I RCCGGY 1870 1874 . . 1870 1875 + BetI* WCCGGW 1870 1874 . . 1870 1875 + AgeI ACCGGT 1870 1874 . . 1871 1874 + HpaII CCGG 1871 1873 . . 1890 1894 - TspDTI ATGAA 1880 1878 . . 1906 1910 + MaeIII GTNAC 1905 1910 . . 1903 1907 - TspGWI ACGGA 1893 1891 . . 1909 1914 + Cfr10I RCCGGY 1909 1913 . . 1909 1914 + BetI* WCCGGW 1909 1913 . . 1909 1914 + AgeI ACCGGT 1909 1913 . . 1910 1913 + HpaII CCGG 1910 1912 . . 1929 1933 - TspDTI ATGAA 1919 1917 . . 1925 1936 - CspCI CAANNNNNGTGG 1949 1947 1914 1912 1945 1949 + MaeIII GTNAC 1944 1949 . . 1942 1946 - TspGWI ACGGA 1932 1930 . . 1948 1953 + Cfr10I RCCGGY 1948 1952 . . 1948 1953 + BetI* WCCGGW 1948 1952 . . 1948 1953 + AgeI ACCGGT 1948 1952 . . 1949 1952 + HpaII CCGG 1949 1951 . . 1963 1968 + Hpy166II GTNNAC 1965 1965 . . 1978 1981 + MaeI CTAG 1978 1980 . . 2002 2006 + Tsp4CI* ACNGT 2004 2003 . . 2020 2024 + Hpy188I TCNGA 2022 2021 . . 2023 2027 - BsmAI GTCTC 2017 2021 . . 2032 2036 + HinfI GANTC 2032 2035 . . 2032 2036 - PleI GAGTC 2026 2027 . . 2032 2036 - MlyI GAGTC 2026 2026 . . 2042 2046 + MaeIII GTNAC 2041 2046 . . 2045 2048 + TaiI ACGT 2048 2044 . . 2045 2048 + SetI ASST 2048 2044 . . 2045 2048 + MaeII ACGT 2045 2047 . . 2061 2072 + TstI CACNNNNNNTCC 2052 2047 2084 2079 2065 2069 + Tsp4CI* ACNGT 2067 2066 . . 2072 2075 + FatI CATG 2071 2075 . . 2072 2075 + NlaIII CATG 2075 2071 . . 2072 2075 + CviAII CATG 2072 2074 . . 2078 2082 - FokI GGATG 2064 2068 . . 2078 2082 - BseGI GGATG 2077 2075 . . 2087 2090 + AciI CCGC 2087 2089 . . 2108 2112 + TspDTI ATGAA 2123 2121 . . 2113 2117 + BsrI ACTGG 2118 2116 . . 2116 2120 + FokI GGATG 2129 2133 . . 2116 2120 + BseGI GGATG 2122 2120 . . 2120 2124 + Tsp45I GTSAC 2119 2124 . . 2120 2124 + MaeIII GTNAC 2119 2124 . . 2123 2126 + TaiI ACGT 2126 2122 . . 2123 2126 + SetI ASST 2126 2122 . . 2123 2126 + MaeII ACGT 2123 2125 . . 2128 2133 + TatI WGTACW 2128 2132 . . 2129 2132 + Csp6I GTAC 2129 2131 . . 2129 2132 + RsaI GTAC 2130 2130 . . 2137 2142 + Cfr10I RCCGGY 2137 2141 . . 2137 2142 + BetI* WCCGGW 2137 2141 . . 2137 2142 + AgeI ACCGGT 2137 2141 . . 2138 2141 + HpaII CCGG 2138 2140 . . 2149 2153 + MaeIII GTNAC 2148 2153 . . 2158 2162 + Tsp4CI* ACNGT 2160 2159 . . 2164 2168 - MboII GAAGA 2156 2155 . . 2187 2193 + TspRI CASTGNN 2193 2184 . . 2188 2192 + Tsp4CI* ACNGT 2190 2189 . . 2191 2195 + MaeIII GTNAC 2190 2195 . . 2197 2203 + TspRI CASTGNN 2203 2194 . . 2198 2202 + Tsp4CI* ACNGT 2200 2199 . . 2201 2205 + Tsp45I GTSAC 2200 2205 . . 2201 2205 + MaeIII GTNAC 2200 2205 . . 2212 2215 + CviJI RGCY 2213 2213 . . 2216 2220 + MaeIII GTNAC 2215 2220 . . 2227 2231 + Tsp4CI* ACNGT 2229 2228 . . 2230 2234 + Tsp45I GTSAC 2229 2234 . . 2230 2234 + MaeIII GTNAC 2229 2234 . . 2236 2240 + Hpy188I TCNGA 2238 2237 . . 2260 2270 + MwoI GCNNNNNNNGC 2266 2263 . . 2260 2270 + BstAPI GCANNNNNTGC 2266 2263 . . 2266 2276 + BsaXI ACNNNNNCTCC 2256 2253 2286 2283 2289 2295 + TspRI CASTGNN 2295 2286 . . 2290 2294 + Tsp4CI* ACNGT 2292 2291 . . 2293 2297 + Tsp45I GTSAC 2292 2297 . . 2293 2297 + MaeIII GTNAC 2292 2297 . . 2296 2300 + Tsp4CI* ACNGT 2298 2297 . . 2294 2298 - HphI GGTGA 2286 2285 . . 2299 2303 + MaeIII GTNAC 2298 2303 . . 2305 2311 + TspRI CASTGNN 2311 2302 . . 2306 2310 + Tsp4CI* ACNGT 2308 2307 . . 2309 2313 + Tsp45I GTSAC 2308 2313 . . 2309 2313 + MaeIII GTNAC 2308 2313 . . 2320 2323 + CviJI RGCY 2321 2321 . . 2324 2328 + MaeIII GTNAC 2323 2328 . . 2335 2339 + Tsp4CI* ACNGT 2337 2336 . . 2338 2342 + Tsp45I GTSAC 2337 2342 . . 2338 2342 + MaeIII GTNAC 2337 2342 . . 2341 2344 + SetI ASST 2344 2340 . . 2342 2345 + MnlI CCTC 2352 2351 . . 2339 2343 - HphI GGTGA 2331 2330 . . 2344 2348 + Hpy188I TCNGA 2346 2345 . . 2345 2350 + Eco57MI CTGRAG 2366 2364 . . 2345 2350 + Eco57I CTGAAG 2366 2364 . . 2349 2352 + SetI ASST 2352 2348 . . 2349 2352 + CviJI RGCY 2350 2350 . . 2349 2352 + AluI AGCT 2350 2350 . . 2350 2360 + MwoI GCNNNNNNNGC 2356 2353 . . 2352 2357 + Hpy178III* TCNNGA 2353 2355 . . 2354 2359 + Eco57MI CTGRAG 2375 2373 . . 2354 2359 + Eco57I CTGAAG 2375 2373 . . 2358 2361 + SetI ASST 2361 2357 . . 2358 2361 + CviJI RGCY 2359 2359 . . 2358 2361 + AluI AGCT 2359 2359 . . 2374 2378 + Tsp4CI* ACNGT 2376 2375 . . 2380 2384 - MboII GAAGA 2372 2371 . . 2391 2396 + Hpy166II GTNNAC 2393 2393 . . 2391 2394 + Csp6I GTAC 2391 2393 . . 2391 2394 + RsaI GTAC 2392 2392 . . 2397 2403 + TspRI CASTGNN 2403 2394 . . 2397 2402 - BtsI GCAGTG 2396 2394 . . 2404 2408 + Tsp4CI* ACNGT 2406 2405 . . 2407 2411 + Tsp45I GTSAC 2406 2411 . . 2407 2411 + MaeIII GTNAC 2406 2411 . . 2409 2414 + TaqII CACCCA 2425 2423 . . 2408 2412 - HphI GGTGA 2400 2399 . . 2413 2419 + TspRI CASTGNN 2419 2410 . . 2413 2422 + MslI CAYNNNNRTG 2417 2417 . . 2414 2418 + Tsp4CI* ACNGT 2416 2415 . . 2428 2432 + FokI GGATG 2441 2445 . . 2428 2432 + BseGI GGATG 2434 2432 . . 2443 2447 + Tsp4CI* ACNGT 2445 2444 . . 2446 2450 + Tsp45I GTSAC 2445 2450 . . 2446 2450 + MaeIII GTNAC 2445 2450 . . 2452 2456 + Hpy188I TCNGA 2454 2453 . . 2453 2458 + Eco57MI CTGRAG 2474 2472 . . 2453 2458 + Eco57I CTGAAG 2474 2472 . . 2457 2460 + SetI ASST 2460 2456 . . 2457 2460 + CviJI RGCY 2458 2458 . . 2457 2460 + AluI AGCT 2458 2458 . . 2458 2468 + MwoI GCNNNNNNNGC 2464 2461 . . 2460 2465 + Hpy178III* TCNNGA 2461 2463 . . 2462 2467 + Eco57MI CTGRAG 2483 2481 . . 2462 2467 + Eco57I CTGAAG 2483 2481 . . 2466 2469 + CviJI RGCY 2467 2467 . . 2479 2483 + Tsp4CI* ACNGT 2481 2480 . . 2479 2489 + BsaXI ACNNNNNCTCC 2469 2466 2499 2496 2504 2507 + AciI CCGC 2504 2506 . . 2509 2513 + Tsp4CI* ACNGT 2511 2510 . . 2512 2516 + MaeIII GTNAC 2511 2516 . . 2516 2520 + DdeI CTNAG 2516 2519 . . 2516 2520 + BspCNI CTCAG 2529 2527 . . 2516 2520 + BseMII CTCAG 2530 2528 . . 2518 2524 + TspRI CASTGNN 2524 2515 . . 2533 2537 + FokI GGATG 2546 2550 . . 2533 2537 + BseGI GGATG 2539 2537 . . 2548 2552 + Tsp4CI* ACNGT 2550 2549 . . 2551 2555 + Tsp45I GTSAC 2550 2555 . . 2551 2555 + MaeIII GTNAC 2550 2555 . . 2557 2561 + Hpy188I TCNGA 2559 2558 . . 2565 2570 + Hpy178III* TCNNGA 2566 2568 . . 2567 2572 + Eco57MI CTGRAG 2588 2586 . . 2567 2572 + Eco57I CTGAAG 2588 2586 . . 2569 2573 + MboII GAAGA 2581 2580 . . 2576 2581 - Eco57MI CTGRAG 2561 2559 . . 2576 2581 - Eco57I CTGAAG 2561 2559 . . 2587 2597 + BsaXI ACNNNNNCTCC 2577 2574 2607 2604 2611 2615 + Tsp4CI* ACNGT 2613 2612 . . 2614 2618 + MaeIII GTNAC 2613 2618 . . 2617 2621 + Tsp4CI* ACNGT 2619 2618 . . 2620 2624 + MaeIII GTNAC 2619 2624 . . 2626 2632 + TspRI CASTGNN 2632 2623 . . 2627 2631 + Tsp4CI* ACNGT 2629 2628 . . 2630 2634 + Tsp45I GTSAC 2629 2634 . . 2630 2634 + MaeIII GTNAC 2629 2634 . . 2639 2643 + BceAI ACGGC 2655 2657 . . 2641 2644 + CviJI RGCY 2642 2642 . . 2645 2649 + MaeIII GTNAC 2644 2649 . . 2651 2655 + DdeI CTNAG 2651 2654 . . 2656 2660 + Tsp4CI* ACNGT 2658 2657 . . 2659 2663 + Tsp45I GTSAC 2658 2663 . . 2659 2663 + MaeIII GTNAC 2658 2663 . . 2662 2665 + SetI ASST 2665 2661 . . 2663 2666 + MnlI CCTC 2673 2672 . . 2660 2664 - HphI GGTGA 2652 2651 . . 2665 2669 + Hpy188I TCNGA 2667 2666 . . 2666 2670 + DdeI CTNAG 2666 2669 . . 2669 2675 + DraII RGGNCCY 2670 2673 . . 2670 2673 + CviJI RGCY 2671 2671 . . 2666 2670 - BspCNI CTCAG 2658 2656 . . 2670 2673 + HaeIII GGCC 2671 2671 . . 2666 2670 - BseMII CTCAG 2657 2655 . . 2670 2675 + NlaIV GGNNCC 2672 2672 . . 2670 2674 + BmgT120I GGNCC 2671 2672 . . 2670 2674 + AsuI* GGNCC 2670 2673 . . 2668 2671 - MnlI CCTC 2661 2660 . . 2671 2681 + MwoI GCNNNNNNNGC 2677 2674 . . 2675 2680 + Eco57MI CTGRAG 2696 2694 . . 2675 2680 + Eco57I CTGAAG 2696 2694 . . 2679 2682 + CviJI RGCY 2680 2680 . . 2692 2696 + Tsp4CI* ACNGT 2694 2693 . . 2692 2702 + BsaXI ACNNNNNCTCC 2682 2679 2712 2709 2717 2720 + AciI CCGC 2717 2719 . . 2722 2726 + Tsp4CI* ACNGT 2724 2723 . . 2725 2729 + MaeIII GTNAC 2724 2729 . . 2729 2733 + DdeI CTNAG 2729 2732 . . 2729 2733 + BspCNI CTCAG 2742 2740 . . 2729 2733 + BseMII CTCAG 2743 2741 . . 2731 2737 + TspRI CASTGNN 2737 2728 . . 2735 2740 + BtgZI GCGATG 2750 2754 . . 2746 2750 + FokI GGATG 2759 2763 . . 2746 2750 + BseGI GGATG 2752 2750 . . 2761 2765 + Tsp4CI* ACNGT 2763 2762 . . 2764 2768 + Tsp45I GTSAC 2763 2768 . . 2764 2768 + MaeIII GTNAC 2763 2768 . . 2770 2774 + Hpy188I TCNGA 2772 2771 . . 2771 2776 + Eco57MI CTGRAG 2792 2790 . . 2771 2776 + Eco57I CTGAAG 2792 2790 . . 2775 2778 + SetI ASST 2778 2774 . . 2775 2778 + CviJI RGCY 2776 2776 . . 2775 2778 + AluI AGCT 2776 2776 . . 2791 2795 + Hpy188I TCNGA 2793 2792 . . 2789 2794 - Eco57MI CTGRAG 2774 2772 . . 2789 2794 - Eco57I CTGAAG 2774 2772 . . 2800 2804 + Hpy188I TCNGA 2802 2801 . . 2798 2803 - Eco57MI CTGRAG 2783 2781 . . 2798 2803 - Eco57I CTGAAG 2783 2781 . . 2808 2814 + TspRI CASTGNN 2814 2805 . . 2807 2811 - BsrI ACTGG 2807 2805 . . 2812 2816 + BlsI GCNGC 2814 2813 . . 2812 2816 + BisI GCNGC 2813 2814 . . 2812 2816 + TseI GCWGC 2812 2815 . . 2812 2816 - BbvI GCAGC 2799 2803 . . 2818 2824 + SauI* CCTNAGG 2819 2822 . . 2819 2823 + DdeI CTNAG 2819 2822 . . 2839 2845 + TspRI CASTGNN 2845 2836 . . 2840 2844 + BsrI ACTGG 2845 2843 . . 2844 2848 + MaeIII GTNAC 2843 2848 . . 2842 2847 - TsoI TARCCA 2832 2830 . . 2854 2858 + HphI GGTGA 2866 2865 . . 2870 2874 + MaeIII GTNAC 2869 2874 . . 2873 2876 + TaiI ACGT 2876 2872 . . 2873 2876 + SetI ASST 2876 2872 . . 2873 2876 + MaeII ACGT 2873 2875 . . 2876 2881 + Hpy178III* TCNNGA 2877 2879 . . 2887 2890 + SetI ASST 2890 2886 . . 2888 2891 + MnlI CCTC 2898 2897 . . 2885 2889 - HphI GGTGA 2877 2876 . . 2898 2901 + SetI ASST 2901 2897 . . 2900 2905 + Eco57MI CTGRAG 2921 2919 . . 2900 2905 + Eco57I CTGAAG 2921 2919 . . 2904 2907 + SetI ASST 2907 2903 . . 2904 2907 + CviJI RGCY 2905 2905 . . 2904 2907 + AluI AGCT 2905 2905 . . 2914 2918 + Tsp4CI* ACNGT 2916 2915 . . 2917 2921 + Tsp45I GTSAC 2916 2921 . . 2917 2921 + MaeIII GTNAC 2916 2921 . . 2922 2932 + MwoI GCNNNNNNNGC 2928 2925 . . 2922 2932 + BstAPI GCANNNNNTGC 2928 2925 . . 2925 2930 + SpeI ACTAGT 2925 2929 . . 2926 2929 + MaeI CTAG 2926 2928 . . 2952 2956 + Tsp4CI* ACNGT 2954 2953 . . 2955 2959 + Tsp45I GTSAC 2954 2959 . . 2955 2959 + MaeIII GTNAC 2954 2959 . . 2961 2965 + Hpy188I TCNGA 2963 2962 . . 2962 2966 + DdeI CTNAG 2962 2965 . . 2962 2966 - BspCNI CTCAG 2954 2952 . . 2962 2966 - BseMII CTCAG 2953 2951 . . 2964 2967 - MnlI CCTC 2957 2956 . . 2979 2982 + SetI ASST 2982 2978 . . #--------------------------------------- # # # AarI: # [CACCTGC] # 1250 1738 # # Acc65I: # [GGTACC] # 594 1105 1289 # # AccI: # [GTMKAC] # 283 1061 1644 # # AciI: # [CCGC] # 30 42 182 186 213 258 # 271 363 417 444 582 # # AflII: # [CTTAAG] # 195 1041 1752 # # AgeI: # [ACCGGT] # 30 39 39 189 192 504 # 851 1144 # # AhaIII*: # [TTTAAA] # 14 2974 # # AluI: # [AGCT] # 6 9 9 9 16 16 # 16 16 16 16 17 17 # 17 17 17 25 25 25 # 25 25 26 26 26 26 # 26 28 36 36 42 48 # 70 83 99 110 129 218 # 318 635 667 # # AlwNI: # [CAGNNNCTG] # 42 42 42 84 84 349 # 792 1553 # # ApoI: # [RAATTY] # 19 1092 1877 # # AsuI*: # [GGNCC] # 42 208 318 2420 # # AvaI: # [CYCGRG] # 237 2751 # # AvaII: # [GGWCC] # 250 2738 # # BalI: # [TGGCCA] # 1486 1502 # # BamHI: # [GGATCC] # 139 2849 # # BbvCI: # [CCTCAGC] # 42 126 468 2352 # # BbvI: # [GCAGC] # 15 149 189 251 743 1641 # # BccI: # [CCATC] # 261 291 643 1793 # # Bce83I*: # [CTTGAG] # 42 42 42 42 42 42 # 84 84 84 102 258 2124 # # BceAI: # [ACGGC] # 333 2655 # # BcgI: # [CGANNNNNNTGC] # 34 1414 1540 # # BetI*: # [WCCGGW] # 21 30 39 39 54 123 # 138 186 189 195 303 820 # 851 # # BinI*: # [GGATC] # 13 134 2841 # # BisI: # [GCNGC] # 29 174 175 251 627 718 # 1014 # # BlsI: # [GCNGC] # 30 174 174 251 627 718 # 1014 # # BmeT110I: # [CYCGRG] # 238 2750 # # BmgT120I: # [GGNCC] # 43 208 317 2420 # # BplI: # [GAGNNNNNCTC] # 0 0 32 160 2796 # # Bpu10I: # [CCTNAGC] # 42 126 468 2352 # # BsaXI: # [ACNNNNNCTCC] # 30 30 30 30 30 75 # 78 183 276 966 1260 # # BseBI: # [CCWGG] # 1103 1885 # # BseGI: # [GGATG] # 33 42 45 45 48 63 # 105 109 213 236 270 312 # 381 462 624 # # BseMII: # [CTCAG] # 35 42 86 126 127 158 # 210 324 408 1472 # # BsiYI*: # [CCNNNNNNNGG] # 144 1409 1435 # # BsmAI: # [GTCTC] # 24 36 42 42 42 42 # 42 77 84 84 84 163 # 283 385 587 971 # # BspCNI: # [CTCAG] # 34 42 84 126 129 159 # 212 322 408 1472 # # BspMI: # [ACCTGC] # 1250 1738 # # BsrI: # [ACTGG] # 38 74 90 143 463 525 # 689 966 # # BssKI: # [CCNGG] # 1101 1887 # # BstAPI: # [GCANNNNNTGC] # 60 132 444 662 819 871 # # BstEII: # [GGTNACC] # 1465 1523 # # BstKTI: # [GATC] # 10 142 799 2037 # # BstXI: # [CCANNNNNNTGG] # 120 2868 # # BtgZI: # [GCGATG] # 238 923 1827 # # BtrI: # [CACGTC] # 1140 1848 # # BtsI: # [GCAGTG] # 189 585 592 1622 # # Cac8I: # [GCNNGC] # 876 2112 # # Cfr10I: # [RCCGGY] # 30 39 39 189 192 504 # 851 1144 # # CfrI: # [YGGCCR] # 1488 1500 # # Csp6I: # [GTAC] # 36 57 72 192 198 262 # 303 429 597 842 # # CspCI: # [CAANNNNNGTGG] # 35 1039 1914 # # CviAII: # [CATG] # 916 2072 # # CviJI: # [RGCY] # 6 6 9 9 9 9 # 9 9 9 16 16 16 # 16 16 16 17 17 17 # 17 17 25 25 25 25 # 25 26 26 26 26 26 # 27 28 29 29 33 36 # 48 64 81 83 96 99 # 108 110 129 132 175 218 # 454 498 # # CviRI*: # [TGCA] # 61 69 84 102 207 480 # 813 1172 # # DdeI: # [CTNAG] # 15 26 42 63 90 126 # 135 143 167 301 408 1472 # # DpnI: # [GATC] # 10 141 799 2038 # # DraII: # [RGGNCCY] # 318 2670 # # DrdI: # [GACNNNNNNGTC] # 177 669 880 1262 # # Ecl136II: # [GAGCTC] # 242 2746 # # Eco57I: # [CTGAAG] # 9 9 9 9 27 47 # 67 78 78 84 99 108 # 129 168 204 267 322 326 # 336 612 # # Eco57MI: # [CTGRAG] # 9 9 9 9 12 27 # 47 67 78 78 84 99 # 108 129 168 204 267 310 # 326 336 612 # # EcoRI: # [GAATTC] # 1111 1877 # # EcoRII: # [CCWGG] # 1101 1887 # # EcoRV: # [GATATC] # 192 2796 # # Esp3I: # [CGTCTC] # 385 1254 1349 # # FalI: # [AAGNNNNNCTT] # 0 0 32 1439 1517 # # FatI: # [CATG] # 917 2071 # # FokI: # [GGATG] # 33 42 45 48 63 65 # 96 105 213 229 270 312 # 381 462 624 # # GlaI: # [GCGC] # 879 2109 # # GsuI: # [CTGGAG] # 1293 1695 # # HaeIII: # [GGCC] # 43 317 1169 1459 # # HgaI: # [GACGC] # 1272 1716 # # HgiAI*: # [GWGCWC] # 244 2744 # # HgiCI*: # [GGYRCC] # 102 189 405 1003 1289 # # HgiJII*: # [GRGCYC] # 244 2744 # # HhaI: # [GCGC] # 880 2108 # # Hin4II*: # [CCTTC] # 45 52 57 159 210 230 # 976 1259 # # Hin6I: # [GCGC] # 878 2110 # # HindII: # [GTYRAC] # 90 1446 1452 # # HinfI: # [GANTC] # 33 151 293 541 956 1014 # # HpaII: # [CCGG] # 21 30 39 39 54 123 # 138 186 189 195 303 821 # 850 # # HphI: # [GGTGA] # 11 36 39 45 60 69 # 81 99 111 123 138 214 # 252 752 958 # # Hpy166II: # [GTNNAC] # 32 75 90 283 423 428 # 595 1062 # # Hpy178III*: # [TCNNGA] # 100 105 108 111 128 137 # 311 917 1071 # # Hpy188I: # [TCNGA] # 9 15 21 25 42 42 # 42 42 42 42 42 42 # 42 105 105 108 108 108 # 161 216 288 309 1032 # # KpnI: # [GGTACC] # 594 1109 1285 # # Ksp632I*: # [CTCTTC] # 36 264 2688 # # MaeI: # [CTAG] # 62 185 397 527 869 948 # # MaeII: # [ACGT] # 78 115 211 695 750 1139 # # MaeIII: # [GTNAC] # 6 6 7 10 10 10 # 14 14 14 15 15 15 # 26 29 30 34 38 39 # 39 39 39 39 39 41 # 42 47 63 63 66 66 # 69 74 78 78 80 97 # 164 199 207 1027 # # MboI: # [GATC] # 10 139 799 2040 # # MboII: # [GAAGA] # 3 3 3 3 3 3 # 3 3 36 36 36 39 # 42 78 123 209 216 228 # 251 281 360 407 622 # # MlyI: # [GAGTC] # 962 1008 1018 # # MmeI: # [TCCRAC] # 914 2074 # # MnlI: # [CCTC] # 6 10 12 18 18 27 # 30 31 42 42 42 42 # 42 59 71 81 84 84 # 90 99 225 309 360 1164 # # MseI: # [TTAA] # 13 154 183 1041 1597 # # MslI: # [CAYNNNNRTG] # 118 571 2299 # # MwoI: # [GCNNNNNNNGC] # 27 34 57 60 68 90 # 108 132 177 183 213 227 # 235 250 251 432 444 # # NlaIII: # [CATG] # 913 2075 # # NlaIV: # [GGNNCC] # 102 141 189 316 405 864 # 971 # # NspBII*: # [CMGCKG] # 48 123 444 1000 1373 # # OliI: # [CACNNNNGTG] # 118 2870 # # PflMI: # [CCANNNNNTGG] # 1435 1553 # # PleI: # [GAGTC] # 962 1008 1018 # # PmeI: # [GTTTAAAC] # 14 2974 # # PvuII: # [CAGCTG] # 48 1000 1940 # # RsaI: # [GTAC] # 36 57 73 192 198 262 # 303 429 596 842 # # SacI: # [GAGCTC] # 244 2744 # # SauI*: # [CCTNAGG] # 169 2819 # # ScaI: # [AGTACT] # 73 2915 # # ScrFI: # [CCNGG] # 1103 1885 # # SduI: # [GDGCHC] # 244 2744 # # SecI*: # [CCNNGG] # 1320 1668 # # SetI: # [ASST] # 5 6 6 6 8 8 # 9 9 9 9 11 14 # 15 16 16 16 16 16 # 16 17 17 17 17 17 # 17 17 18 18 18 25 # 25 25 25 25 26 26 # 26 26 26 26 26 30 # 32 35 35 36 36 38 # 42 43 44 48 52 58 # 75 78 98 99 101 113 # 144 173 185 205 218 279 # # SfaNI: # [GCATC] # 318 633 874 1163 # # SfeI*: # [CTRYAG] # 75 2913 # # SmlI: # [CTYRAG] # 36 42 42 42 42 42 # 42 42 66 84 84 84 # 195 393 1752 # # SpeI: # [ACTAGT] # 63 1133 1792 # # SspI: # [AATATT] # 1036 1952 # # StyI: # [CCWWGG] # 1320 1668 # # TaiI: # [ACGT] # 78 112 211 695 750 1142 # # TaqI: # [TCGA] # 90 115 148 194 877 1564 # # TaqII: # [GACCGA] # 238 563 2187 # # TatI: # [WGTACW] # 71 860 2057 # # TauI: # [GCSGC] # 1187 1801 # # TfiI: # [GAWTC] # 33 541 1165 1249 # # TseI: # [GCWGC] # 28 174 176 251 718 1641 # # TsoI: # [TARCCA] # 156 1261 1571 # # Tsp45I: # [GTSAC] # 16 29 29 29 34 38 # 39 39 41 63 69 74 # 79 81 85 105 105 153 # 853 1027 # # Tsp4CI*: # [ACNGT] # 6 6 10 10 10 10 # 29 29 29 29 30 30 # 30 30 34 36 36 38 # 39 39 39 39 57 60 # 63 63 63 65 93 105 # 133 153 156 362 1027 # # TspDTI: # [ATGAA] # 39 39 45 78 204 209 # 865 1509 # # TspEI: # [AATT] # 19 38 73 80 101 102 # 134 176 213 745 1307 # # TspGWI: # [ACGGA] # 30 39 39 132 159 1056 # 1533 # # TspRI: # [CASTGNN] # 10 16 16 31 36 77 # 92 92 105 105 108 141 # 143 189 225 279 375 948 # # TstI: # [CACNNNNNNTCC] # 32 32 32 262 466 904 # 1260 # # XhoI: # [CTCGAG] # 237 2751 # # XhoII: # [RGATCY] # 139 2849 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 2988 # Reported_sequences: 1 # Reported_hitcount: 834 #---------------------------------------