seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.22309EMBOSS_001 Hin4II* |MaeII TaqII || SetI CviJI DdeI | Hpy178III* || TaiI | TspGWI |SetI | | BsiYI* \\ \ \ \ \\ \ \ \ ATGACGGACGTTGAAGGCTACCAACCTAAGAGCAAGGGGAAAATCTTTCCAGATATGGGT 10 20 30 40 50 60 ----:----|----:----|----:----|----:----|----:----|----:----| TACTGCCTGCAACTTCCGATGGTTGGATTCTCGTTCCCCTTTTAGAAAGGTCTATACCCA // / / / / / // || MaeII | SetI DdeI TaqII |BsiYI* |TaiI TspGWI Hpy178III* |SetI CviJI Hin4II* M T D V E G Y Q P K S K G K I F P D M G * R T L K A T N L R A R G K S F Q I W V D G R * R L P T * E Q G E N L S R Y G * ----:----|----:----|----:----|----:----|----:----|----:----| X V S T S P * W G L L L P F I K G S I P X S P R Q L S G V * S C P S F R E L Y P H R V N F A V L R L A L P F D K W I H T MnlI | Hpy188I | | TfiI | | HinfI | | | SfaNI | | | | AluI | | | | CviJI | | | | | SetI | | | | | |AlwNI | | | | | || MwoI | | | | | || | CviRI* | | | | | || | | MboI | | | | | || | | | DpnI PleI | | | | | || | | | |TaqI |MlyI | | | | | || | | | |ClaI HinfI |HphI | | HphI | | | || | | | |BstKTI \ \\ \ \ \ \ \ \ \\ \ \ \ \\ GAGTCCTTTTTCTCATCAGATGAGGATTCACCAGCTACTGATGCAGAGATCGATGAGAAC 70 80 90 100 110 120 ----:----|----:----|----:----|----:----|----:----|----:----| CTCAGGAAAAAGAGTAGTCTACTCCTAAGTGGTCGATGACTACGTCTCTAGCTACTCTTG / // / / / / / / / / // // | || | | HphI | | | MwoI | || |ClaI | || | Hpy188I | | AlwNI | || |TaqI | || MnlI | | CviJI | || MboI | |PleI | | SfaNI | |DpnI | |MlyI | | AluI | BstKTI | HphI | SetI CviRI* HinfI HinfI TfiI E S F F S S D E D S P A T D A E I D E N S P F S H Q M R I H Q L L M Q R S M R T V L F L I R * G F T S Y * C R D R * E L ----:----|----:----|----:----|----:----|----:----|----:----| S D K K E D S S S E G A V S A S I S S F H T R K R M L H P N V L * Q H L S R H S L G K E * * I L I * W S S I C L D I L V Cfr10I |HpaII || KasI || HgiCI* || |AcyI || |NarI || |Hin6I || ||GlaI || ||DinI || ||NlaIV MaeII || |||HhaI Hin4II* || ||||FatI | SetI || ||||NcoI | TaiI || ||||StyI | | Hpy188I || ||||SecI* | | | MaeII || ||||DsaI* | | | |BtrI || ||||HaeII | | | || SetI || |||||CviAII | | | || TaiI || |||||| MaeIII | | | || | BciVI || |||||| NlaIII \ \ \ \\ \ \ \\ \\\\\\ \ TATGATGATAACAGGGAAACGTCAGAAGGACGTGGAGAAAGGGATACCGGCGCCATGGTA 130 140 150 160 170 180 ----:----|----:----|----:----|----:----|----:----|----:----| ATACTACTATTGTCCCTTTGCAGTCTTCCTGCACCTCTTTCCCTATGGCCGCGGTACCAT / / / / // / ///// // | | | | || BciVI ||||| |DsaI* | | | | |MaeII ||||| |SecI* | | | | BtrI ||||| |StyI | | | TaiI ||||| |NcoI | | | SetI ||||| |FatI | | Hpy188I ||||| CviAII | MaeII ||||HgiCI* Hin4II* ||||NlaIII TaiI ||||KasI SetI |||Hin6I |||NarI |||AcyI ||NlaIV ||DinI ||GlaI |Cfr10I |HhaI HpaII HaeII Y D D N R E T S E G R G E R D T G A M V M M I T G K R Q K D V E K G I P A P W * * * * Q G N V R R T W R K G Y R R H G N ----:----|----:----|----:----|----:----|----:----|----:----| * S S L L S V D S P R P S L S V P A M T S H H Y C P F T L L V H L F P Y R R W P I I I V P F R * F S T S F P I G A G H Y AciI NspBII* |BisI CviJI ||BlsI |SecI* |||AciI || Hpy188I |||TauI || |MboII |||| TfiI || || MnlI Cac8I |||| HinfI \\ \\ \ \ \\\\ \ ACAGGGTTGAAGAAGCCTCGGAAAAAGACCAAGAGTAGCAGGCATACAGCGGCGGATTCC 190 200 210 220 230 240 ----:----|----:----|----:----|----:----|----:----|----:----| TGTCCCAACTTCTTCGGAGCCTTTTTCTGGTTCTCATCGTCCGTATGTCGCCGCCTAAGG / / // / / /// / / MaeIII | || MnlI Cac8I ||| | HinfI | |MboII ||| | TfiI | |SecI* ||| AciI | Hpy188I ||BisI CviJI ||AciI |BlsI NspBII* TauI T G L K K P R K K T K S S R H T A A D S Q G * R S L G K R P R V A G I Q R R I P R V E E A S E K D Q E * Q A Y S G G F L ----:----|----:----|----:----|----:----|----:----|----:----| V P N F F G R F F V L L L L C V A A S E L L T S S A E S F S W S Y C A Y L P P N C P Q L L R P F L G L T A P M C R R I G Hin6I |GlaI TfiI TspDTI |Eco47III HinfI |DdeI ||HhaI |EciI |Bpu10I |||HaeII || MnlI || HgaI |||| CviRI* \\ \ \\ \ \\\\ \ TCTATGAATCAAATGGACGCTAAGGATAAAGCGCTTTTGCAGGACACTAATAGCGATATA 250 260 270 280 290 300 ----:----|----:----|----:----|----:----|----:----|----:----| AGATACTTAGTTTACCTGCGATTCCTATTTCGCGAAAACGTCCTGTGATTATCGCTATAT / // / / //// / | |HinfI | Bpu10I |||Hin6I CviRI* | |TfiI | DdeI ||Eco47III | MnlI TspDTI ||GlaI EciI |HhaI HaeII HgaI S M N Q M D A K D K A L L Q D T N S D I L * I K W T L R I K R F C R T L I A I Y Y E S N G R * G * S A F A G H * * R Y T ----:----|----:----|----:----|----:----|----:----|----:----| E I F * I S A L S L A S K C S V L L S I R * S D F P R * P Y L A K A P C * Y R Y R H I L H V S L I F R K Q L V S I A I Y Hpy188I Hin4I | Hin4I | TspGWI Hpy178III* | | CviJI | | Hpy178III* |BsmAI | | |FatI | | | TfiI |Esp3I | | ||CviAII | | | HinfI || EcoP15I | | ||| NlaIII \ \ \ \ \\ \ \ \ \\\ \ CCAGCAGATTTCGTTCCAGATTCCGTCTCTGGAATGTTCAGAAGCCATGACTTTAGTTAT 310 320 330 340 350 360 ----:----|----:----|----:----|----:----|----:----|----:----| GGTCGTCTAAAGCAAGGTCTAAGGCAGAGACCTTACAAGTCTTCGGTACTGAAATCAATA / / / / / / / / // // | TspGWI | HinfI | | | | || |FatI Hin4I | TfiI | | | | || CviAII Hpy178III* | | | | |NlaIII | | | | CviJI | | | Hpy188I | | Hin4I | EcoP15I | Esp3I | BsmAI Hpy178III* P A D F V P D S V S G M F R S H D F S Y Q Q I S F Q I P S L E C S E A M T L V I S R F R S R F R L W N V Q K P * L * L F ----:----|----:----|----:----|----:----|----:----|----:----| G A S K T G S E T E P I N L L W S K L * V L L N R E L N R R Q F T * F G H S * N W C I E N W I G D R S H E S A M V K T I FatI |CviAII || NlaIII || | BssKI || | EcoRII || | | ScrFI || | | BseBI || | | | AsuI* || | | | DraII || | | | |CviJI || | | | |HaeIII || | | | |BmgT120I || | | | || MboI || | | | || XhoII || | | | || | DpnI || | | | || | |BstKTI || | | | || | || BinI* || | | | || | || | MaeIII || | | | || | || | Tsp45I || | | | || | || | | BsiYI* AciI || | | | || | || | | | Tsp4CI* | MseI || | | | ||NlaIV | || | | | | MboI \ \ \\ \ \ \ \\\ \ \\ \ \ \ \ \ TTGCGGTTAAGACCAGACCATGCTTCCAGGCCCCTTTGGATCTCGCCAAGTGACGGTAGG 370 380 390 400 410 420 ----:----|----:----|----:----|----:----|----:----|----:----| AACGCCAATTCTGGTCTGGTACGAAGGTCCGGGGAAACCTAGAGCGGTTCACTGCCATCC / / / // / /// // / / / / / / / | MseI | |FatI | ||DraII || | | | | | | BstKTI AciI | CviAII | ||AsuI* || | | | | | BplI NlaIII | || || | | | | | BplI | || || | | | | BplI | || || | | | | BplI | || || | | | Tsp4CI* | || || | | | Tsp45I | || || | | | MaeIII | || || | | BsiYI* | || || | BinI* | || || XhoII | || || MboI | || |DpnI | || BstKTI | |BmgT120I | |NlaIV | EcoRII | HaeIII | BssKI | CviJI BseBI ScrFI L R L R P D H A S R P L W I S P S D G R C G * D Q T M L P G P F G S R Q V T V G A V K T R P C F Q A P L D L A K * R * D ----:----|----:----|----:----|----:----|----:----|----:----| K R N L G S W A E L G R Q I E G L S P L N A T L V L G H K W A G K S R A L H R Y Q P * S W V M S G P G K P D R W T V T P GsuI Eco57MI | BplI | BplI | |MwoI | ||BplI | ||BplI | ||| CviJI | ||| |DdeI | ||| |Bpu10I | ||| || Hpy178III* BplI | ||| || | HphI BplI | ||| || | | DrdI DpnI | ||| || | | | MaeIII |BstKTI | ||| || | | | Tsp45I ||BplI | ||| || | | | BstEII ||BplI | ||| || | | | BspCNI ||| BinI* | ||| || | | | |BseMII ||| Hpy178III* | ||| || | | | || BsrDI \\\ \ \ \\\ \\ \ \ \ \\ \ ATCATTCTGGAGAGTTTCTCTCCATTAGCAGAACAGGCTCAGGACTTTCTGGTCACCATT 430 440 450 460 470 480 ----:----|----:----|----:----|----:----|----:----|----:----| TAGTAAGACCTCTCAAAGAGAGGTAATCGTCTTGTCCGAGTCCTGAAAGACCAGTGGTAA / / // / / // / // / / // / / | MboI |Hpy178III* | | |MwoI | || | | || | BstEII DpnI BinI* | | BplI | || | | || | Tsp45I | | BplI | || | | || | MaeIII | BplI | || | | || BsrDI | BplI | || | | |BseMII Eco57MI | || | | BspCNI GsuI | || | DrdI | || HphI | |Hpy178III* | Bpu10I | DdeI CviJI I I L E S F S P L A E Q A Q D F L V T I S F W R V S L H * Q N R L R T F W S P L H S G E F L S I S R T G S G L S G H H C ----:----|----:----|----:----|----:----|----:----|----:----| I M R S L K E G N A S C A * S K R T V M S * E P S N R E M L L V P E P S E P * W D N Q L T E R W * C F L S L V K Q D G N MslI |FatI |MnlI |BspHI ||CviAII ||Hpy178III* ||| MboI AccI ||| | DpnI CviRI* |Hpy166II ||| | |BstKTI | CviJI || TspDTI ||| NlaIII | || TspDTI \ \ \\ \ \\\ \ \ \\ \ GCAGAGCCTATAAGTAGACCCTCCCATATTCATGAATACAAGATCACAGCATACTCTTTG 490 500 510 520 530 540 ----:----|----:----|----:----|----:----|----:----|----:----| CGTCTCGGATATTCATCTGGGAGGGTATAAGTACTTATGTTCTAGTGTCGTATGAGAAAC / / // // // // / / | CviJI |TspDTI || |BspHI || TspDTI MmeI CviRI* |AccI || |FatI || MboI Hpy166II || | |DpnI || | BstKTI || Hpy178III* || CviAII |NlaIII MnlI MslI A E P I S R P S H I H E Y K I T A Y S L Q S L * V D P P I F M N T R S Q H T L C R A Y K * T L P Y S * I Q D H S I L F V ----:----|----:----|----:----|----:----|----:----|----:----| A S G I L L G E W I * S Y L I V A Y E K Q L A * L Y V R G Y E H I C S * L M S K C L R Y T S G G M N M F V L D C C V R Q MmeI | TseI | CviRI* | |BisI | ||BlsI | |||AluI | |||CviJI | |||PvuII | |||NspBII* FokI | |||| SfeI* Hin4I | |||| | BbvI Hin4I | |||| | | Esp3I EcoRV TspGWI | |||| SetI | | BsmAI BseGI | Hpy178III* \ \\\\ \ \ \ \ \ \ \ TATGCAGCTGTTTCTGTAGGGTTGGAGACGGATGATATCATTTCAGTTCTGGATAGATTA 550 560 570 580 590 600 ----:----|----:----|----:----|----:----|----:----|----:----| ATACGTCGACAAAGACATCCCAACCTCTGCCTACTATAGTAAAGTCAAGACCTATCTAAT //// / / / / / / / / / |||NspBII* | | BsmAI | | | | | Hpy178III* |||PvuII | | Esp3I | | | | FokI |||CviJI | BbvI | | | TspGWI |||TseI SfeI* | | Hin4I |||AluI | | Hin4I ||BisI | EcoRV |BlsI BseGI |SetI CviRI* Y A A V S V G L E T D D I I S V L D R L M Q L F L * G W R R M I S F Q F W I D Y C S C F C R V G D G * Y H F S S G * I I ----:----|----:----|----:----|----:----|----:----|----:----| Y A A T E T P N S V S S I M E T R S L N T H L Q K Q L T P S P H Y * K L E P Y I I C S N R Y P Q L R I I D N * N Q I S * Hin4I Hin4I | TfiI | HinfI | | TaqI Csp6I | | ClaI TspDTI |RsaI | | | TspDTI MseI | CviJI \\ \ \ \ \ \ \ \ TCCAAAGTACCCGTTGCTGAATCGATAATCAACTTCATTAAAGGGGCTACCATTTCATAC 610 620 630 640 650 660 ----:----|----:----|----:----|----:----|----:----|----:----| AGGTTTCATGGGCAACGACTTAGCTATTAGTTGAAGTAATTTCCCCGATGGTAAAGTATG /// / / / / / / ||Hin4I | ClaI | | CviJI Tsp4CI* ||Hin4I | TaqI | TspDTI |Csp6I TspDTI MseI RsaI HinfI TfiI S K V P V A E S I I N F I K G A T I S Y P K Y P L L N R * S T S L K G L P F H T Q S T R C * I D N Q L H * R G Y H F I R ----:----|----:----|----:----|----:----|----:----|----:----| D L T G T A S D I I L K M L P A V M E Y I W L V R Q Q I S L * S * * L P * W K M G F Y G N S F R Y D V E N F P S G N * V SpeI Tsp4CI* |MaeI MseI BsmAI \ \\ \ \ GGTAAAGTGAAACTAGTTATTAAGCATAATAGATATTTCGTGGAGACTACACAAGCAGAT 670 680 690 700 710 720 ----:----|----:----|----:----|----:----|----:----|----:----| CCATTTCACTTTGATCAATAATTCGTATTATCTATAAAGCACCTCTGATGTGTTCGTCTA // / / |SpeI MseI BsmAI MaeI G K V K L V I K H N R Y F V E T T Q A D V K * N * L L S I I D I S W R L H K Q I * S E T S Y * A * * I F R G D Y T S R Y ----:----|----:----|----:----|----:----|----:----|----:----| P L T F S T I L C L L Y K T S V V C A S R Y L S V L * * A Y Y I N R P S * V L L T F H F * N N L M I S I E H L S C L C I BsiYI* | AsuI* | Bsp120I | |AsuI* | |DraII | |BmgT120I | ||CviJI | ||NlaIV | ||HaeIII | ||BmgT120I | |||NlaIV | ||||ApaI CviRI* | ||||SduI BsaBI | MwoI | ||||BseSI |MboI | BstAPI TfiI | ||||HgiJII* || DpnI | | TspGWI HinfI | ||||| DdeI || |BstKTI \ \ \ \ \ \\\\\ \ \\ \\ ATTCTGCAAATGTTGCTGAATGATTCCGTCATCGGGCCCCTAAGAATAGATAGCGATCAT 730 740 750 760 770 780 ----:----|----:----|----:----|----:----|----:----|----:----| TAAGACGTTTACAACGACTTACTAAGGCAGTAGCCCGGGGATTCTTATCTATCGCTAGTA / / / / / / /// / / // / | | TspGWI | | | ||| DdeI | || MboI | BstAPI | | | ||Bsp120I | |DpnI | MwoI | | | ||DraII | BstKTI CviRI* | | | ||AsuI* BsaBI | | | |BmgT120I | | | |AsuI* | | | |NlaIV | | | BmgT120I | | | HaeIII | | | NlaIV | | | CviJI | | HgiJII* | | BseSI | | SduI | | ApaI | BsiYI* HinfI TfiI I L Q M L L N D S V I G P L R I D S D H F C K C C * M I P S S G P * E * I A I I S A N V A E * F R H R A P K N R * R S S ----:----|----:----|----:----|----:----|----:----|----:----| I R C I N S F S E T M P G R L I S L S * Y E A F T A S H N R * R A G L F L Y R D N Q L H Q Q I I G D D P G * S Y I A I M TatI |Csp6I TatI |Eco57I |Csp6I |Eco57MI ||RsaI ||RsaI ||| MnlI |||BseGI AciI ||| CviJI ||||SfeI* FokI | NspBII* AciI \\\ \ \\\\\ \ \ \ \ CAAGTACAGCCACCAGAGGATGTACTACAGCAACAACTTCAGCAAACCGCTGGAAAACCC 790 800 810 820 830 840 ----:----|----:----|----:----|----:----|----:----|----:----| GTTCATGTCGGTGGTCTCCTACATGATGTCGTTGTTGAAGTCGTTTGGCGACCTTTTGGG ///// / //// / / / ||||CviJI | |||| SfeI* FokI NspBII* |||MnlI | |||TatI AciI ||TatI | ||Csp6I |Csp6I | |RsaI RsaI | BseGI Eco57MI Eco57I Q V Q P P E D V L Q Q Q L Q Q T A G K P K Y S H Q R M Y Y S N N F S K P L E N P S T A T R G C T T A T T S A N R W K T R ----:----|----:----|----:----|----:----|----:----|----:----| * T C G G S S T S C C C S * C V A P F G D L V A V L P H V V A V V E A F R Q F V L Y L W W L I Y * L L L K L L G S S F G BceAI FauI MseI |Hpy188I CviJI CviRI* SetI FauI \ \ \\ \ \ \ \ GCTACTAATGTTAATCCGAACGATGTGGAAGCCGTGTTTAGTGCAGTTATAGGTGGTGAT 850 860 870 880 890 900 ----:----|----:----|----:----|----:----|----:----|----:----| CGATGATTACAATTAGGCTTGCTACACCTTCGGCACAAATCACGTCAATATCCACCACTA / / / / / / / / / AciI FauI | | BceAI CviJI CviRI* SetI BsgI | Hpy188I MseI A T N V N P N D V E A V F S A V I G G D L L M L I R T M W K P C L V Q L * V V I Y * C * S E R C G S R V * C S Y R W * * ----:----|----:----|----:----|----:----|----:----|----:----| A V L T L G F S T S A T N L A T I P P S R * * H * D S R H P L R T * H L * L H H S S I N I R V I H F G H K T C N Y T T I BdaI BdaI BceAI BsgI | SfaNI | AciI | |MboII TspEI | BsrBI | || MboII | BdaI | |HphI | || | MboII Hpy166II | BdaI \ \\ \ \\ \ \ \ \ \ AATGAGCGGGAAGAAGAAGATGATGATATTGATGCCGTTCACTCCTTTGAAATTGCCAAT 910 920 930 940 950 960 ----:----|----:----|----:----|----:----|----:----|----:----| TTACTCGCCCTTCTTCTTCTACTACTATAACTACGGCAAGTGAGGAAACTTTAACGGTTA / / / / / // / / / / FauI | AciI | | || MboII Hpy166II | TspEI BsrBI | | |SfaNI BdaI HphI | | MboII BdaI | MboII | BceAI BdaI BdaI N E R E E E D D D I D A V H S F E I A N M S G K K K M M I L M P F T P L K L P M * A G R R R * * Y * C R S L L * N C Q * ----:----|----:----|----:----|----:----|----:----|----:----| L S R S S S S S S I S A T * E K S I A L Y H A P L L L H H Y Q H R E S R Q F Q W I L P F F F I I I N I G N V G K F N G I Hpy178III* PleI | TaqI HinfI |MlyI |MmeI ClaI \ \\ \\ \ GAGTCTGTTGAAGTCGTAAAGAAAAGATGTCAAGAAATCGATTATCCCGTGTTGGAAGAA 970 980 990 1000 1010 1020 ----:----|----:----|----:----|----:----|----:----|----:----| CTCAGACAACTTCAGCATTTCTTTTCTACAGTTCTTTAGCTAATAGGGCACAACCTTCTT / / / / / HinfI PleI | | ClaI MlyI | | TaqI | Hpy178III* MmeI E S V E V V K K R C Q E I D Y P V L E E S L L K S * R K D V K K S I I P C W K N V C * S R K E K M S R N R L S R V G R I ----:----|----:----|----:----|----:----|----:----|----:----| S D T S T T F F L H * S I S * G T N S S H T Q Q L R L S F I D L F R N D R T P L L R N F D Y L F S T L F D I I G H Q F F BssKI | HpaII | ScrFI MboII | CauII* SetI \ \ \ \ TACGACTTTAGAAACGACCATAGAAACCCGGATTTAGATATTGATTTGAAACCTTCCACT 1030 1040 1050 1060 1070 1080 ----:----|----:----|----:----|----:----|----:----|----:----| ATGCTGAAATCTTTGCTGGTATCTTTGGGCCTAAATCTATAACTAAACTTTGGAAGGTGA / /// / MboII ||BssKI SetI |HpaII CauII* ScrFI Y D F R N D H R N P D L D I D L K P S T T T L E T T I E T R I * I L I * N L P L R L * K R P * K P G F R Y * F E T F H S ----:----|----:----|----:----|----:----|----:----|----:----| Y S K L F S W L F G S K S I S K F G E V I R S * F R G Y F G P N L Y Q N S V K W V V K S V V M S V R I * I N I Q F R G S Hpy178III* Cac8I Hin4II* | BseMII | SduI | Hpy188I | |BspCNI DdeI | HgiAI* \ \ \ \\ \ \ \ CAAATCAGACCCTATCAAGAAAAATCGCTGAGTAAAATGTTTGGTAATGGGCGTGCTCGT 1090 1100 1110 1120 1130 1140 ----:----|----:----|----:----|----:----|----:----|----:----| GTTTAGTCTGGGATAGTTCTTTTTAGCGACTCATTTTACAAACCATTACCCGCACGAGCA / / // / / | Hpy188I |Hpy178III* DdeI HgiAI* Hin4II* |BspCNI Cac8I BseMII SduI Q I R P Y Q E K S L S K M F G N G R A R K S D P I K K N R * V K C L V M G V L V N Q T L S R K I A E * N V W * W A C S F ----:----|----:----|----:----|----:----|----:----|----:----| * I L G * * S F D S L L I N P L P R A R E F * V R D L F I A S Y F T Q Y H A H E L D S G I L F F R Q T F H K T I P T S T FatI BspMI AciI |CviAII | TseI || NlaIII | |BisI || | Hin6I | ||BlsI || | |GlaI | |||FatI || | ||HhaI SetI | ||||CviAII \\ \ \\\ \ \ \\\\\ TCAGGGATTATTGTTTTGCCATGTGGCGCAGGTAAAACTTTGGTCGGTATTACCGCAGCA 1150 1160 1170 1180 1190 1200 ----:----|----:----|----:----|----:----|----:----|----:----| AGTCCCTAATAACAAAACGGTACACCGCGTCCATTTTGAAACCAGCCATAATGGCGTCGT / // //// //// | || |||SetI |||NlaIII | || ||Hin6I |||TseI | || |GlaI |||NspI | || HhaI ||BisI | |BspMI |BlsI | |FatI AciI | CviAII NlaIII S G I I V L P C G A G K T L V G I T A A Q G L L F C H V A Q V K L W S V L P Q H R D Y C F A M W R R * N F G R Y Y R S M ----:----|----:----|----:----|----:----|----:----|----:----| E P I I T K G H P A P L V K T P I V A A N L S * Q K A M H R L Y F K P R Y * R L * P N N N Q W T A C T F S Q D T N G C C TatI BsmAI |NspI | FatI |Csp6I TspDTI | |CviAII |NlaIII | TatI | || MnlI ||RsaI | |Csp6I | || |CviRI* ||| TspGWI | ||RsaI | || |NlaIII ||| |BbvI TspEI | ||| TspGWI | || || MwoI \\\ \\ \ \ \\\ \ \ \\ \\ \ TGTACTATAAAAAAATCCGTAATTGTTTTATGTACTTCATCAGTCTCCGTCATGCAATGG 1210 1220 1230 1240 1250 1260 ----:----|----:----|----:----|----:----|----:----|----:----| ACATGATATTTTTTTAGGCATTAACAAAATACATGAAGTAGTCAGAGGCAGTACGTTACC ///// / / /// / /// / / ||||TatI BbvI TspDTI ||TatI | ||| | BsrDI |||TspGWI TspEI |TspGWI | ||| MwoI |||Csp6I |Csp6I | ||CviRI* ||RsaI RsaI | ||FatI |FatI | |CviAII CviAII | BsmAI | MnlI NlaIII C T I K K S V I V L C T S S V S V M Q W V L * K N P * L F Y V L H Q S P S C N G Y Y K K I R N C F M Y F I S L R H A M E ----:----|----:----|----:----|----:----|----:----|----:----| H V I F F D T I T K H V E D T E T M C H M Y * L F I R L Q K I Y K M L R R * A I T S Y F F G Y N N * T S * * D G D H L P BsrDI | TseI Csp6I | |BisI Hpy166II | ||BlsI |RsaI SetI SetI | ||| TspEI BbvI || SetI | TspEI HphI | Hpy188I \ \\\ \ \ \\ \ \ \ \ \ \ AGGCAGCAATTTTTACAATGGTGTACCTTACAACCTGAAAATTGTGCTGTTTTCACCTCT 1270 1280 1290 1300 1310 1320 ----:----|----:----|----:----|----:----|----:----|----:----| TCCGTCGTTAAAAATGTTACCACATGGAATGTTGGACTTTTAACACGACAAAAGTGGAGA /// / / /// / // / / ||TseI TspEI | ||Csp6I SetI |HphI SetI Hpy188I |BisI | |RsaI TspEI BlsI | |SetI | Hpy166II BbvI R Q Q F L Q W C T L Q P E N C A V F T S G S N F Y N G V P Y N L K I V L F S P L A A I F T M V Y L T T * K L C C F H L * ----:----|----:----|----:----|----:----|----:----|----:----| L C C N K C H H V K C G S F Q A T K V E S A A I K V I T Y R V V Q F N H Q K * R P L L K * L P T G * L R F I T S N E G R Hpy178III* | TfiI | HinfI | | BetI* MnlI | | |HpaII XcmI \ \ \ \\ \ GATAATAAGGAAATGTTCCAGACAGAATCCGGTTTGGTTGTTTCCACTTATTCAATGGTG 1330 1340 1350 1360 1370 1380 ----:----|----:----|----:----|----:----|----:----|----:----| CTATTATTCCTTTACAAGGTCTGTCTTAGGCCAAACCAACAAAGGTGAATAAGTTACCAC / / / // / MnlI | | |BetI* XcmI | | HpaII | HinfI | TfiI Hpy178III* D N K E M F Q T E S G L V V S T Y S M V I I R K C S R Q N P V W L F P L I Q W W * * G N V P D R I R F G C F H L F N G G ----:----|----:----|----:----|----:----|----:----|----:----| S L L S I N W V S D P K T T E V * E I T Q Y Y P F T G S L I R N P Q K W K N L P I I L F H E L C F G T Q N N G S I * H H SetI | FatI | BspHI | |CviAII | |Hpy178III* Hpy178III* | ||BsmAI | AloI | ||Eco31I | AgeI | ||| TfiI | BetI* | ||| HinfI | Cfr10I | ||| NlaIII | |HpaII TspDTI \ \\\ \ \ \\ \ GCAAACACAAGAAACAGGTCTCATGATTCACAAAAAGTTATGGATTTCTTGACCGGTAGG 1390 1400 1410 1420 1430 1440 ----:----|----:----|----:----|----:----|----:----|----:----| CGTTTGTGTTCTTTGTCCAGAGTACTAAGTGTTTTTCAATACCTAAAGAACTGGCCATCC / / // // / / // / SetI | || |HinfI | | || TspDTI | || |TfiI | | |Cfr10I | || Eco31I | | |BetI* | || BsmAI | | |AgeI | |BspHI | | HpaII | |FatI | Hpy178III* | Hpy178III* AloI | CviAII NlaIII A N T R N R S H D S Q K V M D F L T G R Q T Q E T G L M I H K K L W I S * P V G K H K K Q V S * F T K S Y G F L D R * G ----:----|----:----|----:----|----:----|----:----|----:----| A F V L F L D * S E C F T I S K K V P L P L C L F C T E H N V F L * P N R S R Y C V C S V P R M I * L F N H I E Q G T P FatI |CviAII || NlaIII || | HgiCI* || | | NlaIV || | | | AciI || | | | SduI || | | | Cac8I || | | | BseSI || | | | | TspGWI || | | | | |AciI || | | | | |BisI || | | | | |BdaI || | | | | |BdaI TspDTI || | | | | ||BlsI BsrDI BdaI Hpy178III* || | | | | |||TauI | BtgZI BdaI | AloI || | | | | |||| FauI | | BsiYI* \ \ \ \\ \ \ \ \ \\\\ \ \ \ \ GAATGGGGGTTCATTATTCTTGACGAAGTTCATGTGGTGCCCGCCGCAATGTTCCGTAGG 1450 1460 1470 1480 1490 1500 ----:----|----:----|----:----|----:----|----:----|----:----| CTTACCCCCAAGTAATAAGAACTGCTTCAAGTACACCACGGGCGGCGTTACAAGGCATCC / / / / / // // /////// // / BdaI | | | | || || ||||||| |BsrDI BsiYI* BdaI | | | | || || ||||||| FauI | | | | || || ||||||AciI | | | | || || |||||BisI | | | | || || ||||BlsI | | | | || || |||AciI | | | | || || |||TauI | | | | || || ||BdaI | | | | || || ||BdaI | | | | || || |TspGWI | | | | || || |Cac8I | | | | || || HgiCI* | | | | || |NlaIV | | | | || BseSI | | | | || SduI | | | | |FatI | | | | CviAII | | | NlaIII | | Hpy178III* | AloI TspDTI E W G F I I L D E V H V V P A A M F R R N G G S L F L T K F M W C P P Q C S V G M G V H Y S * R S S C G A R R N V P * G ----:----|----:----|----:----|----:----|----:----|----:----| S H P N M I R S S T * T T G A A I N R L P I P T * * E Q R L E H P A R R L T G Y F P P E N N K V F N M H H G G C H E T P TseI |BisI ||BlsI BsmAI |||BccI BbvI TsoI Eco31I |||| MwoI TspEI |AciI BslFI \ \\\\ \ \ \\ \ GTGGTCTCCACCATCGCAGCACACGCCAAATTGGGACTGACCGCAACGCTGGTTAGAGAA 1510 1520 1530 1540 1550 1560 ----:----|----:----|----:----|----:----|----:----|----:----| CACCAGAGGTGGTAGCGTCGTGTGCGGTTTAACCCTGACTGGCGTTGCGACCAATCTCTT / / //// / / / / BtgZI | |||BccI TspEI TsoI AciI BslFI | ||MwoI BbvI | ||TseI | |BisI | BlsI Eco31I BsmAI V V S T I A A H A K L G L T A T L V R E W S P P S Q H T P N W D * P Q R W L E K G L H H R S T R Q I G T D R N A G * R R ----:----|----:----|----:----|----:----|----:----|----:----| T T E V M A A C A L N P S V A V S T L S P P R W W R L V R W I P V S R L A P * L H D G G D C C V G F Q S Q G C R Q N S F TspEI | MboII | | MboI | | | DpnI | | | |BstKTI | | | || Hpy188I | | | || |ApoI | | | || |TspEI | | | || || HphI | | | || || | MseI | | | || || | |TspEI | | | || || | || AsuI* | | | || || | || AvaII BccI | | | || || | || |BmgT120I TspEI \ \ \ \\ \\ \ \\ \\ \ GATGATAAAATTGGTGATCTGAATTTTTTAATTGGTCCAAAACTTTATGAAGCAAATTGG 1570 1580 1590 1600 1610 1620 ----:----|----:----|----:----|----:----|----:----|----:----| CTACTATTTTAACCACTAGACTTAAAAAATTAACCAGGTTTTGAAATACTTCGTTTAACC / / // / // / / // / / / | | || | || | | |AvaII | | TspDTI | | || | || | | |AsuI* | TspEI | | || | || | | BmgT120I BccI | | || | || | TspEI | | || | || MseI | | || | |TspEI | | || | |ApoI | | || | HphI | | || Hpy188I | | || MboI | | |DpnI | | BstKTI | TspEI MboII D D K I G D L N F L I G P K L Y E A N W M I K L V I * I F * L V Q N F M K Q I G * * N W * S E F F N W S K T L * S K L D ----:----|----:----|----:----|----:----|----:----|----:----| S S L I P S R F K K I P G F S * S A F Q L H Y F Q H D S N K L Q D L V K H L L N I I F N T I Q I K * N T W F K I F C I P TspDTI MnlI | TspEI | BslFI FatI | |BseGI FokI CviRI* | | CviRI* SetI |CviAII \ \\ \ \ \ \ \ \ \\ ATGGAATTATCCCAAAAAGGACATATTGCAAATGTCCAATGTGCAGAGGTTTGGTGTCCC 1630 1640 1650 1660 1670 1680 ----:----|----:----|----:----|----:----|----:----|----:----| TACCTTAATAGGGTTTTTCCTGTATAACGTTTACAGGTTACACGTCTCCAAACCACAGGG / / / / / / // // | TspEI FokI CviRI* MnlI | |SetI |BsgI BseGI | BslFI NlaIII CviRI* M E L S Q K G H I A N V Q C A E V W C P W N Y P K K D I L Q M S N V Q R F G V P G I I P K R T Y C K C P M C R G L V S H ----:----|----:----|----:----|----:----|----:----|----:----| I S N D W F P C I A F T W H A S T Q H G S P I I G F L V Y Q L H G I H L P K T D H F * G L F S M N C I D L T C L N P T G BsgI ApoI EcoP15I BsmI |NlaIII TspEI MseI | CviRI* | Tsp4CI* \\ \ \ \ \ \ \ ATGACAGCAGAATTTTACCAAGAGTATTTAAGAGAAACTGCAAGGAAAAGAATGCTACTG 1690 1700 1710 1720 1730 1740 ----:----|----:----|----:----|----:----|----:----|----:----| TACTGTCGTCTTAAAATGGTTCTCATAAATTCTCTTTGACGTTCCTTTTCTTACGATGAC // / / / / / / |FatI TspEI MseI | CviRI* | Tsp4CI* CviAII ApoI EcoP15I BsmI M T A E F Y Q E Y L R E T A R K R M L L * Q Q N F T K S I * E K L Q G K E C Y C D S R I L P R V F K R N C K E K N A T V ----:----|----:----|----:----|----:----|----:----|----:----| M V A S N * W S Y K L S V A L F L I S S W S L L I K G L T N L L F Q L S F F A V H C C F K V L L I * S F S C P F S H * Q TspDTI | AjuI | | FatI | | |CviAII | | ||Cac8I FatI | | ||| SphI BspHI | | ||| NspI |CviAII | | ||| NlaIII |Hpy178III* | | ||| | TspEI || TfiI | | ||| | | MmeI || HinfI | | ||| | | | MseI Hpy178III* || NlaIII | | ||| | | | |TspEI |MnlI AjuI \\ \ \ \ \\\ \ \ \ \\ \\ \ TATATCATGAATCCAACAAAGTTTCAGGCATGCCAATTCTTAATTCAATATCACGAAAGG 1750 1760 1770 1780 1790 1800 ----:----|----:----|----:----|----:----|----:----|----:----| ATATAGTACTTAGGTTGTTTCAAAGTCCGTACGGTTAAGAATTAAGTTATAGTGCTTTCC / // / // / /// / / / / /// | || HinfI |AjuI | ||| | | | TspEI ||Hpy178III* | || TfiI TspDTI | ||| | | MseI |AjuI | |BspHI | ||| | TspEI MnlI | |FatI | ||| MmeI | Hpy178III* | ||FatI | CviAII | |CviAII NlaIII | Cac8I NlaIII NspI SphI Y I M N P T K F Q A C Q F L I Q Y H E R I S * I Q Q S F R H A N S * F N I T K G Y H E S N K V S G M P I L N S I S R K E ----:----|----:----|----:----|----:----|----:----|----:----| Y I M F G V F N * A H W N K I * Y * S L T Y * S D L L T E P M G I R L E I D R F I D H I W C L K L C A L E * N L I V F P MboI | DpnI | |BstKTI | || HphI Hpy188I Hpy166II \ \\ \ \ \ AGGGGTGATAAGATCATCGTTTTTTCAGATAATGTTTACGCCTTACAAGAATATGCTTTG 1810 1820 1830 1840 1850 1860 ----:----|----:----|----:----|----:----|----:----|----:----| TCCCCACTATTCTAGTAGCAAAAAAGTCTATTACAAATGCGGAATGTTCTTATACGAAAC // / / / || HphI Hpy188I Hpy166II || MboI |DpnI BstKTI R G D K I I V F S D N V Y A L Q E Y A L G V I R S S F F Q I M F T P Y K N M L * G * * D H R F F R * C L R L T R I C F E ----:----|----:----|----:----|----:----|----:----|----:----| L P S L I M T K E S L T * A K C S Y A K S P H Y S * R K K L Y H K R R V L I H K P T I L D D N K * I I N V G * L F I S Q NlaIV CviRI* \ \ AAAATGGGAAAACCATTTATTTATGGTTCCACACCACAACAAGAGCGTATGAACATTCTG 1870 1880 1890 1900 1910 1920 ----:----|----:----|----:----|----:----|----:----|----:----| TTTTACCCTTTTGGTAAATAAATACCAAGGTGTGGTGTTGTTCTCGCATACTTGTAAGAC / / NlaIV CviRI* K M G K P F I Y G S T P Q Q E R M N I L K W E N H L F M V P H H N K S V * T F C N G K T I Y L W F H T T T R A Y E H S A ----:----|----:----|----:----|----:----|----:----|----:----| F I P F G N I * P E V G C C S R I F M R S F P F V M * K H N W V V V L A Y S C E F H S F W K N I T G C W L L L T H V N Q ApoI TspEI |TspDTI || BsrI || | TatI || | |Csp6I || | ||RsaI \\ \ \\\ CAAAATTTCCAGTACAATGACCAAATCAATACCATATTTCTATCAAAAGTTGGTGATACT 1930 1940 1950 1960 1970 1980 ----:----|----:----|----:----|----:----|----:----|----:----| GTTTTAAAGGTCATGTTACTGGTTTAGTTATGGTATAAAGATAGTTTTCAACCACTATGA / / /// | TspEI ||TatI | ApoI |Csp6I | BsrI RsaI TspDTI Q N F Q Y N D Q I N T I F L S K V G D T K I S S T M T K S I P Y F Y Q K L V I L K F P V Q * P N Q Y H I S I K S W * Y F ----:----|----:----|----:----|----:----|----:----|----:----| C F K W Y L S W I L V M N R D F T P S V A F N G T C H G F * Y W I E I L L Q H Y L I E L V I V L D I G Y K * * F N T I S AluI CviJI | SetI | | SetI HphI | | | MseI |TaqI | | | |TspEI BsmAI |ClaI BccI | | | || MboII Eco31I \\ \ \ \ \ \\ \ \ TCCATCGATTTACCAGAAGCTACCTGTTTAATTCAAATATCTTCGCACTATGGGTCTCGT 1990 2000 2010 2020 2030 2040 ----:----|----:----|----:----|----:----|----:----|----:----| AGGTAGCTAAATGGTCTTCGATGGACAAATTAAGTTTATAGAAGCGTGATACCCAGAGCA / / / / / / // / / | | BccI | | SetI || TspEI Hpy99I | ClaI | CviJI |MboII | TaqI | AluI MseI HphI SetI S I D L P E A T C L I Q I S S H Y G S R P S I Y Q K L P V * F K Y L R T M G L V H R F T R S Y L F N S N I F A L W V S S ----:----|----:----|----:----|----:----|----:----|----:----| E M S K G S A V Q K I * I D E C * P D R K W R N V L L * R N L E F I K A S H T E G D I * W F S G T * N L Y R R V I P R T ApoI TspEI | MseI | | MboII MaeII Hpy99I | | | AluI | SetI | Hpy178III* | | | CviJI | TaiI | | CviJI | | | | SetI | |Hin4II* SetI \ \ \ \ \ \ \ \ \ \\ \ CGTCAAGAAGCCCAAAGATTAGGAAGAATTTTAAGAGCTAAAAGACGTAATGACGAAGGT 2050 2060 2070 2080 2090 2100 ----:----|----:----|----:----|----:----|----:----|----:----| GCAGTTCTTCGGGTTTCTAATCCTTCTTAAAATTCTCGATTTTCTGCATTACTGCTTCCA / / / / /// / / // / | | CviJI | ||| CviJI | |Hin4II* SetI | Hpy178III* | ||| AluI | MaeII Eco31I | ||SetI TaiI BsmAI | |MboII SetI | MseI TspEI ApoI R Q E A Q R L G R I L R A K R R N D E G V K K P K D * E E F * E L K D V M T K V S R S P K I R K N F K S * K T * * R R F ----:----|----:----|----:----|----:----|----:----|----:----| R * S A W L N P L I K L A L L R L S S P D D L L G F I L F F K L L * F V Y H R L T L F G L S * S S N * S S F S T I V F T TatI BciVI |Csp6I | DdeI ||RsaI BsmAI \ \ \\\ \ TTCAATGCGTTCTTTTATTCTCTGGTTTCTAAGGATACGCAAGAAATGTACTACTCAACA 2110 2120 2130 2140 2150 2160 ----:----|----:----|----:----|----:----|----:----|----:----| AAGTTACGCAAGAAAATAAGAGACCAAAGATTCCTATGCGTTCTTTACATGATGAGTTGT / / /// BciVI DdeI ||TatI |Csp6I RsaI F N A F F Y S L V S K D T Q E M Y Y S T S M R S F I L W F L R I R K K C T T Q Q Q C V L L F S G F * G Y A R N V L L N K ----:----|----:----|----:----|----:----|----:----|----:----| K L A N K * E R T E L S V C S I Y * E V N * H T R K N E P K * P Y A L F T S S L E I R E K I R Q N R L I R L F H V V * C MboI | DpnI | |BstKTI | || MaeIII | || | SetI | || | | BsmI \ \\ \ \ \ AAGAGACAAGCGTTTTTGGTAGATCAAGGTTACGCATTCAAAGTCATTACACATTTACAC 2170 2180 2190 2200 2210 2220 ----:----|----:----|----:----|----:----|----:----|----:----| TTCTCTGTTCGCAAAAACCATCTAGTTCCAATGCGTAAGTTTCAGTAATGTGTAAATGTG / // // // BsmAI || |SetI |MaeIII || MboI BsmI |DpnI BstKTI K R Q A F L V D Q G Y A F K V I T H L H R D K R F W * I K V T H S K S L H I Y T E T S V F G R S R L R I Q S H Y T F T R ----:----|----:----|----:----|----:----|----:----|----:----| F L C A N K T S * P * A N L T M V C K C L S V L T K P L D L N R M * L * * V N V L S L R K Q Y I L T V C E F D N C M * V MaeII | SetI | TaiI | | TaqII | | | AluI TspGWI | | | CviJI | ApoI HphI | | | | SetI | TspEI |NdeI | | | | | CviRI* \ \ \\ \ \ \ \ \ \ GGAATGGAGAACATTCCAAATTTGGCATATGCTTCACCCAGAGAACGTAGAGAGCTATTG 2230 2240 2250 2260 2270 2280 ----:----|----:----|----:----|----:----|----:----|----:----| CCTTACCTCTTGTAAGGTTTAAACCGTATACGAAGTGGGTCTCTTGCATCTCTCGATAAC / / / / / / / / / / TspGWI | | NdeI | | | | CviJI CviRI* | HphI | | | | AluI TspEI | | | SetI ApoI | | TaqII | MaeII TaiI SetI G M E N I P N L A Y A S P R E R R E L L E W R T F Q I W H M L H P E N V E S Y C N G E H S K F G I C F T Q R T * R A I A ----:----|----:----|----:----|----:----|----:----|----:----| P I S F M G F K A Y A E G L S R L S S N R F P S C E L N P M H K V W L V Y L A I S H L V N W I Q C I S * G S F T S L * Q MboII |TseI |AluI |CviJI ||BisI |||BlsI |||SetI |||| MnlI |||| |MboII SetI HphI EcoP15I BcgI BbvI |||| || TaqI BcgI | TspEI \ \ \ \\\\ \\ \ \ \ \ CAAGAAGTATTATTGAAGAACGAAGAAGCTGCTGGTATCGAGGTCGGTGATGACGCTGAC 2290 2300 2310 2320 2330 2340 ----:----|----:----|----:----|----:----|----:----|----:----| GTTCTTCATAATAACTTCTTGCTTCTTCGACGACCATAGCTCCAGCCACTACTGCGACTG / / / / ////// / / / | BcgI BbvI | |||||MboII | BcgI HphI EcoP15I | ||||MnlI TaqI | |||TseI SetI | ||BisI | |BlsI | CviJI | AluI MboII SetI Q E V L L K N E E A A G I E V G D D A D K K Y Y * R T K K L L V S R S V M T L T R S I I E E R R S C W Y R G R * * R * Q ----:----|----:----|----:----|----:----|----:----|----:----| C S T N N F F S S A A P I S T P S S A S A L L I I S S R L L Q Q Y R P R H H R Q L F Y * Q L V F F S S T D L D T I V S V MnlI BceAI CviJI CfrI | MseI HaeIII | BalI | |SwaI | Hin4II* | CviJI | |BsaBI | |TspDTI HgaI MnlI SetI | HaeIII | |AhaIII* | || SetI \ \ \ \ \ \ \\ \ \\ \ AATTCTGTTGGGAGAGGTTCAAATGGCCACAAAAGATTTAAATCAAAGGCCGTTAGAGGT 2350 2360 2370 2380 2390 2400 ----:----|----:----|----:----|----:----|----:----|----:----| TTAAGACAACCCTCTCCAAGTTTACCGGTGTTTTCTAAATTTAGTTTCCGGCAATCTCCA / / / / / / // // / / | HgaI SetI | CfrI | |MseI || | SetI | MnlI HaeIII | AhaIII* || Hin4II* TspEI CviJI | BsaBI || TspDTI BalI | SwaI |HaeIII BceAI |CviJI MnlI N S V G R G S N G H K R F K S K A V R G I L L G E V Q M A T K D L N Q R P L E V F C W E R F K W P Q K I * I K G R * R * ----:----|----:----|----:----|----:----|----:----|----:----| L E T P L P E F P W L L N L D F A T L P C N Q Q S L N L H G C F I * I L P R * L I R N P S T * I A V F S K F * L G N S T Tsp4CI* SetI Cac8I HphI |Csp6I | HphI | AciI | MboII ||RsaI \ \ \ \ \ \ \\\ GAAGGTTCATTGTCTGGTCTTGCTGGCGGTGAAGATATGGCATATATGGAATACAGTACC 2410 2420 2430 2440 2450 2460 ----:----|----:----|----:----|----:----|----:----|----:----| CTTCCAAGTAACAGACCAGAACGACCGCCACTTCTATACCGTATATACCTTATGTCATGG / / / / / / / // SetI HphI | AciI | MboII | |Csp6I Cac8I HphI | RsaI Tsp4CI* E G S L S G L A G G E D M A Y M E Y S T K V H C L V L L A V K I W H I W N T V P R F I V W S C W R * R Y G I Y G I Q Y Q ----:----|----:----|----:----|----:----|----:----|----:----| S P E N D P R A P P S S I A Y I S Y L V H L N M T Q D Q Q R H L Y P M Y P I C Y F T * Q R T K S A T F I H C I H F V T G MseI VspI | Eco57I PsiI Hin4II* | Eco57MI | ApoI | FokI BseGI | | Hpy188I | TspEI \ \ \ \ \ \ \ \ AATAAAAACAAAGAACTGAAGGAACATCATCCATTAATCAGAAAGATGTATTATAAGAAT 2470 2480 2490 2500 2510 2520 ----:----|----:----|----:----|----:----|----:----|----:----| TTATTTTTGTTTCTTGACTTCCTTGTAGTAGGTAATTAGTCTTTCTACATAATATTCTTA / / / // / / | FokI BseGI || Hpy188I PsiI Hin4II* |VspI |MseI Eco57MI Eco57I N K N K E L K E H H P L I R K M Y Y K N I K T K N * R N I I H * S E R C I I R I * K Q R T E G T S S I N Q K D V L * E F ----:----|----:----|----:----|----:----|----:----|----:----| L L F L S S F S C * G N I L F I Y * L F W Y F C L V S P V D D M L * F S T N Y S I F V F F Q L F M M W * D S L H I I L I TTGAAGAAGTGA 2530 ----:----|-- AACTTCTTCACT / TspEI ApoI L K K * * R S X E E V X ----:----|-- K F F H N S S T Q L L S # Enzymes that cut Frequency Isoschizomers AccI 1 FblI,XmiI AciI 11 BspACI,SsiI AcyI 1 BsaHI,BssNI,BstACI,Hin1I,Hsp92I AgeI 1 AsiGI,BshTI,CspAI,PinAI AhaIII* 1 DraI AjuI 1 AloI 1 AluI 6 AluBI AlwNI 1 CaiI ApaI 1 ApoI 6 AcsI,XapI AsuI* 4 Cfr13I,PspPI,Sau96I,AspS9I AvaII 1 Bme18I,Eco47I,SinI,VpaK11BI BalI 1 MlsI,MluNI,MscI,Msp20I BbvI 5 BseXI,BstV1I,Lsp1109I BccI 3 BceAI 3 BcgI 1 BciVI 2 BfuI BdaI 4 BetI* 2 BsaWI BinI* 2 AlwI,BspPI,AclWI BisI 7 Fnu4HI,Fsp4HI,GluI,ItaI,SatI BlsI 7 BmgT120I 4 BplI 4 Bpu10I 2 BsaBI 2 Bse8I,BseJI BseBI 1 Bst2UI,BstNI,BstOI,MvaI BseGI 4 BstF5I,BtsCI BseMII 2 BseSI 2 BaeGI,BstSLI BsgI 2 BsiYI* 4 Bsc4I,BseLI,BslI,AfiI BslFI 2 BsmFI,FaqI BsmAI 8 Alw26I,BstMAI BsmI 2 BsaMI,Mva1269I,PctI Bsp120I 1 PspOMI BspCNI 2 BspHI 3 CciI,PagI,RcaI BspMI 1 BfuAI,Acc36I,BveI BsrBI 1 AccBSI,MbiI BsrDI 3 BseMI,Bse3DI BsrI 1 BseNI,Bse1I,BsrSI BssKI 2 BstSCI,StyD4I BstAPI 1 BstEII 1 BstPI,Eco91I,EcoO65I,PspEI BstKTI 8 BtgZI 1 BtrI 1 BmgBI,AjiI Cac8I 5 BstC8I CauII* 1 BcnI,BpuMI,NciI,AsuC2I Cfr10I 2 BsrFI,BssAI,Bse118I CfrI 1 AcoI,EaeI ClaI 4 Bsa29I,BseCI,BshVI,BspDI,BspXI,Bsu15I,BsuTUI,BanIII Csp6I 9 CviQI,RsaNI CviAII 12 CviJI 19 CviKI-1 CviRI* 12 HpyCH4V DdeI 6 BstDEI,HpyF3I DinI 1 EgeI,EheI,SfoI DpnI 8 MalI DraII 2 EcoO109I DrdI 1 AasI,DseDI DsaI* 1 BtgI,BstDSI EciI 1 Eco31I 3 Bso31I,BspTNI,BsaI Eco47III 1 Aor51HI,AfeI Eco57I 2 AcuI Eco57MI 3 EcoP15I 3 EcoRII 1 AjnI,Psp6I,PspGI EcoRV 1 Eco32I Esp3I 2 BsmBI FatI 12 FauI 3 SmuI FokI 4 GlaI 3 GsuI 1 BpmI HaeII 2 BstH2I HaeIII 4 BsnI,BsuRI,BshFI,PhoI HgaI 2 CseI HgiAI* 1 Bbv12I,BsiHKAI,Alw21I HgiCI* 2 BanI,BshNI,BspT107I,AccB1I HgiJII* 1 Eco24I,EcoT38I,FriOI,BanII HhaI 3 BstHHI,CfoI,AspLEI Hin4I 3 Hin4II* 6 HpyAV Hin6I 3 HinP1I,HspAI HinfI 11 HpaII 4 HapII,BsiSI,MspI HphI 12 AsuHPI Hpy166II 4 Hpy8I Hpy178III* 16 Hpy188III Hpy188I 10 Hpy99I 1 KasI 1 MaeI 1 FspBI,BfaI,XspI MaeII 5 HpyCH4IV MaeIII 4 MboI 8 Bsp143I,BssMI,BstMBI,DpnII,Kzo9I,BfuCI,NdeII,Sau3AI MboII 11 MlyI 2 SchI MmeI 3 MnlI 12 MseI 11 Tru1I,Tru9I MslI 1 RseI,SmiMI MwoI 5 HpyF10VI,BstMWI NarI 1 Mly113I NcoI 1 Bsp19I NdeI 1 FauNDI NlaIII 12 Hin1II,Hsp92II,FaeI NlaIV 6 BspLI,BmiI,PspN4I NspBII* 3 MspA1I NspI 2 BstNSI,XceI PleI 2 PpsI PsiI 1 AanI PvuII 1 RsaI 9 AfaI ScrFI 2 BmrFI,MspR9I,Bme1390I SduI 3 MhlI,Bsp1286I SecI* 2 BseDI,BssECI,BsaJI SetI 27 SfaNI 2 LweI SfeI* 2 BstSFI,SfcI,BfmI SpeI 1 BcuI,AhlI SphI 1 PaeI,BbuI StyI 1 Eco130I,EcoT14I,ErhI,BssT1I SwaI 1 SmiI TaiI 5 TaqI 5 TaqII 2 TatI 6 TauI 2 TfiI 9 PfeI TseI 5 ApeKI TsoI 1 Tsp45I 2 NmuCI Tsp4CI* 4 HpyCH4III,TaaI,Bst4CI TspDTI 12 TspEI 19 TasI,Tsp509I,Sse9I TspGWI 8 VspI 1 PshBI,AseI XcmI 1 XhoII 1 BstYI,MflI,PsuI,BstX2I # Enzymes which cut less frequently than the MINCUTS criterion # Enzymes < MINCUTS Frequency Isoschizomers # Enzymes which cut more frequently than the MAXCUTS criterion # Enzymes > MAXCUTS Frequency Isoschizomers # Enzymes that do not cut AarI AatII AbsI Acc65I AclI AflII AflIII AlfI ApaLI AscI Asp718I AsuII AvaI AvrII BaeI BamHI BarI BbvCI BbvII* Bce83I* BclI BfiI BglI BglII BmeT110I BmtI BsaAI BsaXI BsePI BseRI BseYI BsiI* Bsp1407I BspLU11I* BspMII* BspOI BssNAI Bst1107I BstXI BstZ17I BtsI Cfr9I CspCI DraIII Eam1105I Ecl136II EcoICRI EcoNI EcoRI EcoT22I EspI* FalI FnuDII* FseI FspAI GsaI HindII HindIII HpaI KpnI Ksp632I* MauBI McrI* MfeI MluI Mph1103I MroNI MstI* NaeI NgoMIV NheI NmeAIII NotI NruI NsiI OliI PacI PasI PflMI PfoI PmaCI PmeI PpiI PpuMI PshAI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SauI* ScaI SexAI SfiI SgfI SgrAI SgrDI SmaI SmlI SnaBI SplI* SrfI Sse232I* Sse8387I SspI StuI TspMI TspRI TstI Tth111I XbaI XhoI XmaCI XmaI XmaIII* XmnI ZraI Zsp2I # No. of cutting enzymes which do not match the # SITELEN, BLUNT, STICKY, COMMERCIAL, AMBIGUOUS criteria 769