Restriction Fragments for SSL2/YIL143C



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The currently selected gene/sequence is :

SSL2/YIL143C on chromosome IX from coordinates 83041 to 80510.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.27611.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 4 8 + TspGWI ACGGA 19 17 . . 8 11 + TaiI ACGT 11 7 . . 8 11 + SetI ASST 11 7 . . 8 11 + MaeII ACGT 8 10 . . 16 19 + CviJI RGCY 17 17 . . 13 17 - Hin4II* CCTTC 7 6 . . 24 27 + SetI ASST 27 23 . . 26 30 + DdeI CTNAG 26 29 . . 48 53 + Hpy178III* TCNNGA 49 51 . . 49 59 + BsiYI* CCNNNNNNNGG 55 52 . . 58 62 + HphI GGTGA 70 69 . . 56 61 - TaqII CACCCA 46 44 . . 61 65 + PleI GAGTC 69 70 . . 61 65 + MlyI GAGTC 70 70 . . 61 65 + HinfI GANTC 61 64 . . 76 80 + Hpy188I TCNGA 78 77 . . 85 89 + TfiI GAWTC 85 88 . . 82 85 - MnlI CCTC 75 74 . . 85 89 + HinfI GANTC 85 88 . . 88 92 - HphI GGTGA 80 79 . . 92 100 + AlwNI CAGNNNCTG 97 94 . . 93 96 + SetI ASST 96 92 . . 93 96 + CviJI RGCY 94 94 . . 93 96 + AluI AGCT 94 94 . . 94 104 + MwoI GCNNNNNNNGC 100 97 . . 102 105 + CviRI* TGCA 103 103 . . 100 104 - SfaNI GCATC 90 94 . . 108 111 + DpnI GATC 109 109 . . 108 111 + BstKTI GATC 110 108 . . 108 111 + MboI GATC 107 111 . . 109 114 + ClaI ATCGAT 110 112 . . 110 113 + TaqI TCGA 110 112 . . 139 142 + TaiI ACGT 142 138 . . 139 142 + SetI ASST 142 138 . . 139 142 + MaeII ACGT 139 141 . . 142 146 + Hpy188I TCNGA 144 143 . . 145 149 - Hin4II* CCTTC 139 138 . . 150 153 + TaiI ACGT 153 149 . . 150 153 + SetI ASST 153 149 . . 150 153 + MaeII ACGT 150 152 . . 149 154 - BtrI CACGTC 151 151 . . 166 171 + Cfr10I RCCGGY 166 170 . . 167 170 + HpaII CCGG 167 169 . . 162 167 - BciVI GTATCC 156 155 . . 169 174 + NarI GGCGCC 170 172 . . 169 174 + KasI GGCGCC 169 173 . . 169 174 + DinI GGCGCC 171 171 . . 169 174 + HaeII RGCGCY 173 169 . . 169 174 + NlaIV GGNNCC 171 171 . . 169 174 + AcyI GRCGYC 170 172 . . 169 174 + HgiCI* GGYRCC 169 173 . . 170 173 + Hin6I GCGC 170 172 . . 170 173 + GlaI GCGC 171 171 . . 170 173 + HhaI GCGC 172 170 . . 173 178 + DsaI* CCRYGG 173 177 . . 173 178 + StyI CCWWGG 173 177 . . 173 178 + NcoI CCATGG 173 177 . . 173 178 + SecI* CCNNGG 173 177 . . 174 177 + FatI CATG 173 177 . . 174 177 + NlaIII CATG 177 173 . . 174 177 + CviAII CATG 174 176 . . 178 182 + MaeIII GTNAC 177 182 . . 189 193 + MboII GAAGA 201 200 . . 194 197 + CviJI RGCY 195 195 . . 196 199 + MnlI CCTC 206 205 . . 196 201 + SecI* CCNNGG 196 200 . . 198 202 + Hpy188I TCNGA 200 199 . . 218 223 + Cac8I GCNNGC 220 220 . . 227 232 + NspBII* CMGCKG 229 229 . . 229 233 + TauI GCSGC 232 229 . . 229 233 + BlsI GCNGC 231 230 . . 229 233 + BisI GCNGC 230 231 . . 231 236 + EciI GGCGGA 247 245 . . 229 232 - AciI CCGC 229 231 . . 235 239 + TfiI GAWTC 235 238 . . 235 239 + HinfI GANTC 235 238 . . 232 235 - AciI CCGC 232 234 . . 239 242 + MnlI CCTC 249 248 . . 244 248 + TspDTI ATGAA 259 257 . . 246 250 + TfiI GAWTC 246 249 . . 246 250 + HinfI GANTC 246 249 . . 256 260 + HgaI GACGC 265 270 . . 260 264 + DdeI CTNAG 260 263 . . 259 265 - Bpu10I CCTNAGC 260 263 . . 270 275 + HaeII RGCGCY 274 270 . . 270 275 + Eco47III AGCGCT 272 272 . . 271 274 + Hin6I GCGC 271 273 . . 271 274 + GlaI GCGC 272 272 . . 271 274 + HhaI GCGC 273 271 . . 278 281 + CviRI* TGCA 279 279 . . 302 307 + EcoP15I CAGCAG 332 334 . . 315 320 + Hpy178III* TCNNGA 316 318 . . 319 323 + TfiI GAWTC 319 322 . . 319 323 + HinfI GANTC 319 322 . . 319 329 + Hin4I GAYNNNNNVTC 310 305 342 337 324 329 + Esp3I CGTCTC 330 334 . . 325 329 + BsmAI GTCTC 330 334 . . 322 326 - TspGWI ACGGA 312 310 . . 328 333 + Hpy178III* TCNNGA 329 331 . . 338 342 + Hpy188I TCNGA 340 339 . . 343 346 + CviJI RGCY 344 344 . . 346 349 + FatI CATG 345 349 . . 346 349 + NlaIII CATG 349 345 . . 346 349 + CviAII CATG 346 348 . . 363 366 - AciI CCGC 363 365 . . 367 370 + MseI TTAA 367 369 . . 379 382 + FatI CATG 378 382 . . 379 382 + NlaIII CATG 382 378 . . 379 382 + CviAII CATG 379 381 . . 386 390 + BssKI CCNGG 385 390 . . 386 390 + BseBI CCWGG 387 388 . . 386 390 + ScrFI CCNGG 387 388 . . 386 390 + EcoRII CCWGG 385 390 . . 388 394 + DraII RGGNCCY 389 392 . . 389 392 + CviJI RGCY 390 390 . . 389 392 + HaeIII GGCC 390 390 . . 389 394 + NlaIV GGNNCC 391 391 . . 389 393 + BmgT120I GGNCC 390 391 . . 389 393 + AsuI* GGNCC 389 392 . . 398 403 + XhoII RGATCY 398 402 . . 398 402 + BinI* GGATC 406 407 . . 399 402 + DpnI GATC 400 400 . . 399 402 + BstKTI GATC 401 399 . . 399 402 + MboI GATC 398 402 . . 406 416 + BsiYI* CCNNNNNNNGG 412 409 . . 410 414 + Tsp45I GTSAC 409 414 . . 410 414 + MaeIII GTNAC 409 414 . . 413 417 + Tsp4CI* ACNGT 415 414 . . 419 423 + BinI* GGATC 427 428 . . 420 423 + DpnI GATC 421 421 . . 420 423 + BstKTI GATC 422 420 . . 420 423 + MboI GATC 419 423 . . 426 431 + Hpy178III* TCNNGA 427 429 . . 427 432 + Eco57MI CTGRAG 448 446 . . 427 432 + GsuI CTGGAG 448 446 . . 430 440 + BplI GAGNNNNNCTC 421 416 453 448 432 442 + BplI GAGNNNNNCTC 423 418 455 450 430 440 - BplI GAGNNNNNCTC 453 448 421 416 432 442 - BplI GAGNNNNNCTC 455 450 423 418 448 458 + MwoI GCNNNNNNNGC 454 451 . . 456 459 + CviJI RGCY 457 457 . . 458 462 + DdeI CTNAG 458 461 . . 458 462 + BspCNI CTCAG 471 469 . . 458 462 + BseMII CTCAG 472 470 . . 459 464 + Hpy178III* TCNNGA 460 462 . . 457 463 - Bpu10I CCTNAGC 458 461 . . 463 474 + DrdI GACNNNNNNGTC 469 467 . . 471 477 + BstEII GGTNACC 471 476 . . 472 476 + Tsp45I GTSAC 471 476 . . 472 476 + MaeIII GTNAC 471 476 . . 473 477 - HphI GGTGA 465 464 . . 480 483 + CviRI* TGCA 481 481 . . 477 482 - BsrDI GCAATG 476 474 . . 485 488 + CviJI RGCY 486 486 . . 494 499 + Hpy166II GTNNAC 496 496 . . 494 499 + AccI GTMKAC 495 497 . . 500 503 + MnlI CCTC 510 509 . . 505 514 + MslI CAYNNNNRTG 509 509 . . 510 515 + Hpy178III* TCNNGA 511 513 . . 510 515 + BspHI TCATGA 510 514 . . 511 514 + FatI CATG 510 514 . . 511 514 + NlaIII CATG 514 510 . . 511 514 + CviAII CATG 511 513 . . 512 516 + TspDTI ATGAA 527 525 . . 509 513 - TspDTI ATGAA 499 497 . . 522 525 + DpnI GATC 523 523 . . 522 525 + BstKTI GATC 524 522 . . 522 525 + MboI GATC 521 525 . . 543 546 + CviRI* TGCA 544 544 . . 544 548 + BlsI GCNGC 546 545 . . 544 548 + BisI GCNGC 545 546 . . 544 548 + BbvI GCAGC 556 560 . . 544 548 + TseI GCWGC 544 547 . . 545 550 + PvuII CAGCTG 547 547 . . 545 550 + NspBII* CMGCKG 547 547 . . 546 549 + SetI ASST 549 545 . . 546 549 + CviJI RGCY 547 547 . . 546 549 + AluI AGCT 547 547 . . 554 559 + SfeI* CTRYAG 554 558 . . 561 566 - MmeI TCCRAC 542 540 . . 568 572 + TspGWI ACGGA 583 581 . . 565 569 - BsmAI GTCTC 559 563 . . 570 574 + FokI GGATG 583 587 . . 565 570 - Esp3I CGTCTC 559 563 . . 570 574 + BseGI GGATG 576 574 . . 574 579 + EcoRV GATATC 576 576 . . 588 593 + Hpy178III* TCNNGA 589 591 . . 592 602 + Hin4I GAYNNNNNVTC 583 578 615 610 592 602 - Hin4I GAYNNNNNVTC 615 610 583 578 607 610 + Csp6I GTAC 607 609 . . 607 610 + RsaI GTAC 608 608 . . 619 623 + TfiI GAWTC 619 622 . . 619 623 + HinfI GANTC 619 622 . . 621 626 + ClaI ATCGAT 622 624 . . 622 625 + TaqI TCGA 622 624 . . 634 638 - TspDTI ATGAA 624 622 . . 638 641 + MseI TTAA 638 640 . . 645 648 + CviJI RGCY 646 646 . . 654 658 - TspDTI ATGAA 644 642 . . 659 663 + Tsp4CI* ACNGT 661 660 . . 672 677 + SpeI ACTAGT 672 676 . . 673 676 + MaeI CTAG 673 675 . . 680 683 + MseI TTAA 680 682 . . 703 707 - BsmAI GTCTC 697 701 . . 725 728 + CviRI* TGCA 726 726 . . 726 736 + MwoI GCNNNNNNNGC 732 729 . . 726 736 + BstAPI GCANNNNNTGC 732 729 . . 742 746 + TfiI GAWTC 742 745 . . 742 746 + HinfI GANTC 742 745 . . 746 756 + BsiYI* CCNNNNNNNGG 752 749 . . 745 749 - TspGWI ACGGA 735 733 . . 754 760 + DraII RGGNCCY 755 758 . . 754 759 + SduI GDGCHC 758 754 . . 754 759 + Bsp120I GGGCCC 754 758 . . 754 759 + NlaIV GGNNCC 756 756 . . 754 758 + BmgT120I GGNCC 755 756 . . 754 759 + HgiJII* GRGCYC 758 754 . . 754 759 + BseSI GKGCMC 758 754 . . 754 758 + AsuI* GGNCC 754 757 . . 754 759 + ApaI GGGCCC 758 754 . . 755 758 + CviJI RGCY 756 756 . . 755 758 + HaeIII GGCC 756 756 . . 755 760 + NlaIV GGNNCC 757 757 . . 755 759 + BmgT120I GGNCC 756 757 . . 755 759 + AsuI* GGNCC 755 758 . . 760 764 + DdeI CTNAG 760 763 . . 769 778 + BsaBI GATNNNNATC 773 773 . . 775 778 + DpnI GATC 776 776 . . 775 778 + BstKTI GATC 777 775 . . 775 778 + MboI GATC 774 778 . . 783 788 + TatI WGTACW 783 787 . . 784 787 + Csp6I GTAC 784 786 . . 784 787 + RsaI GTAC 785 785 . . 788 791 + CviJI RGCY 789 789 . . 798 802 + FokI GGATG 811 815 . . 798 802 + BseGI GGATG 804 802 . . 796 799 - MnlI CCTC 789 788 . . 801 806 + TatI WGTACW 801 805 . . 802 805 + Csp6I GTAC 802 804 . . 802 805 + RsaI GTAC 803 803 . . 805 810 + SfeI* CTRYAG 805 809 . . 817 822 - Eco57MI CTGRAG 802 800 . . 817 822 - Eco57I CTGAAG 802 800 . . 827 832 + NspBII* CMGCKG 829 829 . . 827 830 + AciI CCGC 827 829 . . 838 842 + FauI CCCGC 846 848 . . 839 842 + AciI CCGC 839 841 . . 851 854 + MseI TTAA 851 853 . . 855 859 + Hpy188I TCNGA 857 856 . . 870 873 + CviJI RGCY 871 871 . . 871 875 - BceAI ACGGC 856 858 . . 881 886 + BsgI GTGCAG 902 900 . . 882 885 + CviRI* TGCA 883 883 . . 891 894 + SetI ASST 894 890 . . 895 899 + HphI GGTGA 907 906 . . 906 909 - AciI CCGC 906 908 . . 904 909 - BsrBI CCGCTC 906 906 . . 910 914 + MboII GAAGA 922 921 . . 906 910 - FauI CCCGC 899 901 . . 913 917 + MboII GAAGA 925 924 . . 916 920 + MboII GAAGA 928 927 . . 930 941 + BdaI TGANNNNNNTCA 919 917 953 951 931 935 - SfaNI GCATC 921 925 . . 937 942 + Hpy166II GTNNAC 939 939 . . 934 938 - BceAI ACGGC 919 921 . . 930 941 - BdaI TGANNNNNNTCA 953 951 919 917 951 954 + TspEI AATT 950 954 . . 961 965 + PleI GAGTC 969 970 . . 961 965 + MlyI GAGTC 970 970 . . 961 965 + HinfI GANTC 961 964 . . 990 995 + Hpy178III* TCNNGA 991 993 . . 997 1002 + ClaI ATCGAT 998 1000 . . 998 1001 + TaqI TCGA 998 1000 . . 1015 1019 + MboII GAAGA 1027 1026 . . 1011 1016 - MmeI TCCRAC 992 990 . . 1047 1051 + BssKI CCNGG 1046 1051 . . 1047 1051 + ScrFI CCNGG 1048 1049 . . 1047 1051 + CauII* CCSGG 1048 1049 . . 1048 1051 + HpaII CCGG 1048 1050 . . 1071 1074 + SetI ASST 1074 1070 . . 1072 1076 + Hin4II* CCTTC 1082 1081 . . 1085 1089 + Hpy188I TCNGA 1087 1086 . . 1095 1100 + Hpy178III* TCNNGA 1096 1098 . . 1108 1112 + DdeI CTNAG 1108 1111 . . 1108 1112 - BspCNI CTCAG 1100 1098 . . 1108 1112 - BseMII CTCAG 1099 1097 . . 1131 1136 + Cac8I GCNNGC 1133 1133 . . 1133 1138 + SduI GDGCHC 1137 1133 . . 1133 1138 + HgiAI* GWGCWC 1137 1133 . . 1160 1163 + FatI CATG 1159 1163 . . 1160 1163 + NlaIII CATG 1163 1159 . . 1160 1163 + CviAII CATG 1160 1162 . . 1166 1169 + Hin6I GCGC 1166 1168 . . 1166 1169 + GlaI GCGC 1167 1167 . . 1166 1169 + HhaI GCGC 1168 1166 . . 1170 1173 + SetI ASST 1173 1169 . . 1168 1173 - BspMI ACCTGC 1159 1163 . . 1193 1196 + AciI CCGC 1193 1195 . . 1195 1199 + BlsI GCNGC 1197 1196 . . 1195 1199 + BisI GCNGC 1196 1197 . . 1195 1199 + BbvI GCAGC 1207 1211 . . 1195 1199 + TseI GCWGC 1195 1198 . . 1198 1203 + NspI RCATGY 1202 1198 . . 1199 1202 + FatI CATG 1198 1202 . . 1199 1202 + NlaIII CATG 1202 1198 . . 1199 1202 + CviAII CATG 1199 1201 . . 1201 1206 + TatI WGTACW 1201 1205 . . 1202 1205 + Csp6I GTAC 1202 1204 . . 1202 1205 + RsaI GTAC 1203 1203 . . 1216 1220 - TspGWI ACGGA 1206 1204 . . 1221 1224 + TspEI AATT 1220 1224 . . 1231 1236 + TatI WGTACW 1231 1235 . . 1232 1235 + Csp6I GTAC 1232 1234 . . 1232 1235 + RsaI GTAC 1233 1233 . . 1236 1240 - TspDTI ATGAA 1226 1224 . . 1243 1247 + BsmAI GTCTC 1248 1252 . . 1246 1250 - TspGWI ACGGA 1236 1234 . . 1251 1254 + FatI CATG 1250 1254 . . 1251 1254 + NlaIII CATG 1254 1250 . . 1251 1254 + CviAII CATG 1251 1253 . . 1253 1256 + CviRI* TGCA 1254 1254 . . 1254 1264 + MwoI GCNNNNNNNGC 1260 1257 . . 1254 1259 + BsrDI GCAATG 1261 1259 . . 1260 1263 - MnlI CCTC 1253 1252 . . 1263 1267 + BlsI GCNGC 1265 1264 . . 1263 1267 + BisI GCNGC 1264 1265 . . 1263 1267 + BbvI GCAGC 1275 1279 . . 1263 1267 + TseI GCWGC 1263 1266 . . 1268 1271 + TspEI AATT 1267 1271 . . 1281 1286 + Hpy166II GTNNAC 1283 1283 . . 1283 1286 + Csp6I GTAC 1283 1285 . . 1283 1286 + RsaI GTAC 1284 1284 . . 1285 1288 + SetI ASST 1288 1284 . . 1293 1296 + SetI ASST 1296 1292 . . 1300 1303 + TspEI AATT 1299 1303 . . 1315 1318 + SetI ASST 1318 1314 . . 1316 1319 + MnlI CCTC 1326 1325 . . 1313 1317 - HphI GGTGA 1305 1304 . . 1318 1322 + Hpy188I TCNGA 1320 1319 . . 1337 1342 + Hpy178III* TCNNGA 1338 1340 . . 1345 1349 + TfiI GAWTC 1345 1348 . . 1345 1349 + HinfI GANTC 1345 1348 . . 1348 1353 + BetI* WCCGGW 1348 1352 . . 1349 1352 + HpaII CCGG 1349 1351 . . 1364 1378 + XcmI CCANNNNNNNNNTGG 1371 1370 . . 1396 1399 + SetI ASST 1399 1395 . . 1397 1402 + Eco31I GGTCTC 1403 1407 . . 1398 1402 + BsmAI GTCTC 1403 1407 . . 1401 1406 + Hpy178III* TCNNGA 1402 1404 . . 1401 1406 + BspHI TCATGA 1401 1405 . . 1402 1405 + FatI CATG 1401 1405 . . 1402 1405 + NlaIII CATG 1405 1401 . . 1402 1405 + CviAII CATG 1402 1404 . . 1405 1409 + TfiI GAWTC 1405 1408 . . 1405 1409 + HinfI GANTC 1405 1408 . . 1427 1432 + Hpy178III* TCNNGA 1428 1430 . . 1432 1437 + Cfr10I RCCGGY 1432 1436 . . 1432 1437 + BetI* WCCGGW 1432 1436 . . 1432 1437 + AgeI ACCGGT 1432 1436 . . 1433 1436 + HpaII CCGG 1433 1435 . . 1440 1452 - AloI GAACNNNNNNTCC 1464 1459 1432 1427 1450 1454 - TspDTI ATGAA 1440 1438 . . 1458 1463 + Hpy178III* TCNNGA 1459 1461 . . 1461 1472 + BdaI TGANNNNNNTCA 1450 1448 1484 1482 1471 1474 + FatI CATG 1470 1474 . . 1471 1474 + NlaIII CATG 1474 1470 . . 1471 1474 + CviAII CATG 1471 1473 . . 1461 1472 - BdaI TGANNNNNNTCA 1484 1482 1450 1448 1469 1473 - TspDTI ATGAA 1459 1457 . . 1476 1481 + NlaIV GGNNCC 1478 1478 . . 1476 1481 + HgiCI* GGYRCC 1476 1480 . . 1477 1482 + SduI GDGCHC 1481 1477 . . 1477 1482 + BseSI GKGCMC 1481 1477 . . 1479 1484 + Cac8I GCNNGC 1481 1481 . . 1480 1484 + FauI CCCGC 1488 1490 . . 1481 1484 + AciI CCGC 1481 1483 . . 1483 1487 + TauI GCSGC 1486 1483 . . 1483 1487 + BlsI GCNGC 1485 1484 . . 1483 1487 + BisI GCNGC 1484 1485 . . 1484 1487 + AciI CCGC 1484 1486 . . 1486 1491 + BsrDI GCAATG 1493 1491 . . 1494 1504 + BsiYI* CCNNNNNNNGG 1500 1497 . . 1493 1497 - TspGWI ACGGA 1483 1481 . . 1503 1508 + Eco31I GGTCTC 1509 1513 . . 1504 1508 + BsmAI GTCTC 1509 1513 . . 1511 1515 + BccI CCATC 1519 1520 . . 1516 1526 + MwoI GCNNNNNNNGC 1522 1519 . . 1516 1520 + BlsI GCNGC 1518 1517 . . 1516 1520 + BisI GCNGC 1517 1518 . . 1516 1520 + BbvI GCAGC 1528 1532 . . 1516 1520 + TseI GCWGC 1516 1519 . . 1512 1517 - BtgZI GCGATG 1497 1501 . . 1529 1532 + TspEI AATT 1528 1532 . . 1533 1537 + BslFI GGGAC 1547 1551 . . 1541 1544 + AciI CCGC 1541 1543 . . 1550 1555 - TsoI TARCCA 1540 1538 . . 1558 1562 + MboII GAAGA 1570 1569 . . 1569 1572 + TspEI AATT 1568 1572 . . 1573 1577 + HphI GGTGA 1585 1584 . . 1576 1579 + DpnI GATC 1577 1577 . . 1576 1579 + BstKTI GATC 1578 1576 . . 1576 1579 + MboI GATC 1575 1579 . . 1578 1582 + Hpy188I TCNGA 1580 1579 . . 1581 1586 + ApoI RAATTY 1581 1585 . . 1582 1585 + TspEI AATT 1581 1585 . . 1588 1591 + MseI TTAA 1588 1590 . . 1590 1593 + TspEI AATT 1589 1593 . . 1594 1598 + BmgT120I GGNCC 1595 1596 . . 1594 1598 + AvaII GGWCC 1594 1597 . . 1594 1598 + AsuI* GGNCC 1594 1597 . . 1607 1611 + TspDTI ATGAA 1622 1620 . . 1615 1618 + TspEI AATT 1614 1618 . . 1619 1623 + FokI GGATG 1632 1636 . . 1619 1623 + BseGI GGATG 1625 1623 . . 1620 1624 - BccI CCATC 1614 1615 . . 1625 1628 + TspEI AATT 1624 1628 . . 1647 1650 + CviRI* TGCA 1648 1648 . . 1661 1666 + BsgI GTGCAG 1682 1680 . . 1662 1665 + CviRI* TGCA 1663 1663 . . 1667 1670 + SetI ASST 1670 1666 . . 1666 1669 - MnlI CCTC 1659 1658 . . 1680 1683 + FatI CATG 1679 1683 . . 1680 1683 + NlaIII CATG 1683 1679 . . 1680 1683 + CviAII CATG 1680 1682 . . 1676 1680 - BslFI GGGAC 1661 1665 . . 1685 1690 + EcoP15I CAGCAG 1715 1717 . . 1690 1695 + ApoI RAATTY 1690 1694 . . 1691 1694 + TspEI AATT 1690 1694 . . 1708 1711 + MseI TTAA 1708 1710 . . 1719 1722 + CviRI* TGCA 1720 1720 . . 1730 1735 + BsmI GAATGC 1736 1734 . . 1737 1741 + Tsp4CI* ACNGT 1739 1738 . . 1745 1750 + Hpy178III* TCNNGA 1746 1748 . . 1745 1750 + BspHI TCATGA 1745 1749 . . 1746 1749 + FatI CATG 1745 1749 . . 1746 1749 + NlaIII CATG 1749 1745 . . 1746 1749 + CviAII CATG 1746 1748 . . 1747 1751 + TspDTI ATGAA 1762 1760 . . 1749 1753 + TfiI GAWTC 1749 1752 . . 1749 1753 + HinfI GANTC 1749 1752 . . 1752 1757 + MmeI TCCRAC 1777 1775 . . 1768 1773 + NspI RCATGY 1772 1768 . . 1768 1773 + Cac8I GCNNGC 1770 1770 . . 1768 1773 + SphI GCATGC 1772 1768 . . 1769 1772 + FatI CATG 1768 1772 . . 1769 1772 + NlaIII CATG 1772 1768 . . 1769 1772 + CviAII CATG 1769 1771 . . 1775 1778 + TspEI AATT 1774 1778 . . 1780 1783 + MseI TTAA 1780 1782 . . 1782 1785 + TspEI AATT 1781 1785 . . 1773 1786 - AjuI GAANNNNNNNTTGG 1798 1793 1766 1761 1791 1796 + Hpy178III* TCNNGA 1792 1794 . . 1800 1803 - MnlI CCTC 1793 1792 . . 1804 1808 + HphI GGTGA 1816 1815 . . 1812 1815 + DpnI GATC 1813 1813 . . 1812 1815 + BstKTI GATC 1814 1812 . . 1812 1815 + MboI GATC 1811 1815 . . 1825 1829 + Hpy188I TCNGA 1827 1826 . . 1834 1839 + Hpy166II GTNNAC 1836 1836 . . 1885 1890 + NlaIV GGNNCC 1887 1887 . . 1909 1913 + TspDTI ATGAA 1924 1922 . . 1919 1922 + CviRI* TGCA 1920 1920 . . 1923 1928 + ApoI RAATTY 1923 1927 . . 1924 1927 + TspEI AATT 1923 1927 . . 1931 1936 + TatI WGTACW 1931 1935 . . 1932 1935 + Csp6I GTAC 1932 1934 . . 1932 1935 + RsaI GTAC 1933 1933 . . 1929 1933 - BsrI ACTGG 1929 1927 . . 1972 1976 + HphI GGTGA 1984 1983 . . 1982 1986 + BccI CCATC 1990 1991 . . 1984 1989 + ClaI ATCGAT 1985 1987 . . 1985 1988 + TaqI TCGA 1985 1987 . . 1998 2001 + SetI ASST 2001 1997 . . 1998 2001 + CviJI RGCY 1999 1999 . . 1998 2001 + AluI AGCT 1999 1999 . . 2002 2005 + SetI ASST 2005 2001 . . 2008 2011 + MseI TTAA 2008 2010 . . 2010 2013 + TspEI AATT 2009 2013 . . 2020 2024 - MboII GAAGA 2012 2011 . . 2033 2038 + Eco31I GGTCTC 2039 2043 . . 2034 2038 + BsmAI GTCTC 2039 2043 . . 2038 2042 + Hpy99I CGWCG 2042 2037 . . 2043 2048 + Hpy178III* TCNNGA 2044 2046 . . 2049 2052 + CviJI RGCY 2050 2050 . . 2063 2067 + MboII GAAGA 2075 2074 . . 2066 2071 + ApoI RAATTY 2066 2070 . . 2067 2070 + TspEI AATT 2066 2070 . . 2071 2074 + MseI TTAA 2071 2073 . . 2076 2079 + SetI ASST 2079 2075 . . 2076 2079 + CviJI RGCY 2077 2077 . . 2076 2079 + AluI AGCT 2077 2077 . . 2085 2088 + TaiI ACGT 2088 2084 . . 2085 2088 + SetI ASST 2088 2084 . . 2085 2088 + MaeII ACGT 2085 2087 . . 2097 2100 + SetI ASST 2100 2096 . . 2095 2099 - Hin4II* CCTTC 2089 2088 . . 2129 2133 + DdeI CTNAG 2129 2132 . . 2133 2138 - BciVI GTATCC 2127 2126 . . 2147 2152 + TatI WGTACW 2147 2151 . . 2148 2151 + Csp6I GTAC 2148 2150 . . 2148 2151 + RsaI GTAC 2149 2149 . . 2163 2167 - BsmAI GTCTC 2157 2161 . . 2182 2185 + DpnI GATC 2183 2183 . . 2182 2185 + BstKTI GATC 2184 2182 . . 2182 2185 + MboI GATC 2181 2185 . . 2187 2190 + SetI ASST 2190 2186 . . 2189 2193 + MaeIII GTNAC 2188 2193 . . 2194 2199 - BsmI GAATGC 2194 2192 . . 2219 2223 + TspGWI ACGGA 2234 2232 . . 2238 2243 + ApoI RAATTY 2238 2242 . . 2239 2242 + TspEI AATT 2238 2242 . . 2246 2251 + NdeI CATATG 2247 2249 . . 2255 2260 + TaqII CACCCA 2271 2269 . . 2254 2258 - HphI GGTGA 2246 2245 . . 2265 2268 + TaiI ACGT 2268 2264 . . 2265 2268 + SetI ASST 2268 2264 . . 2265 2268 + MaeII ACGT 2265 2267 . . 2273 2276 + SetI ASST 2276 2272 . . 2273 2276 + CviJI RGCY 2274 2274 . . 2273 2276 + AluI AGCT 2274 2274 . . 2279 2282 + CviRI* TGCA 2280 2280 . . 2295 2299 + MboII GAAGA 2307 2306 . . 2301 2312 + BcgI CGANNNNNNTGC 2290 2288 2324 2322 2302 2306 + MboII GAAGA 2314 2313 . . 2307 2310 + SetI ASST 2310 2306 . . 2307 2310 + CviJI RGCY 2308 2308 . . 2307 2310 + AluI AGCT 2308 2308 . . 2308 2312 + BlsI GCNGC 2310 2309 . . 2308 2312 + BisI GCNGC 2309 2310 . . 2308 2312 + TseI GCWGC 2308 2311 . . 2308 2312 - BbvI GCAGC 2295 2299 . . 2309 2314 - EcoP15I CAGCAG 2281 2283 . . 2318 2321 + TaqI TCGA 2318 2320 . . 2321 2324 + SetI ASST 2324 2320 . . 2320 2323 - MnlI CCTC 2313 2312 . . 2326 2330 + HphI GGTGA 2338 2337 . . 2332 2336 + HgaI GACGC 2341 2346 . . 2341 2344 + TspEI AATT 2340 2344 . . 2355 2358 + SetI ASST 2358 2354 . . 2354 2357 - MnlI CCTC 2347 2346 . . 2364 2369 + BalI TGGCCA 2366 2366 . . 2364 2369 + CfrI YGGCCR 2364 2368 . . 2365 2368 + CviJI RGCY 2366 2366 . . 2365 2368 + HaeIII GGCC 2366 2366 . . 2375 2384 + BsaBI GATNNNNATC 2379 2379 . . 2376 2383 + SwaI ATTTAAAT 2379 2379 . . 2377 2382 + AhaIII* TTTAAA 2379 2379 . . 2378 2381 + MseI TTAA 2378 2380 . . 2388 2391 + CviJI RGCY 2389 2389 . . 2388 2391 + HaeIII GGCC 2389 2389 . . 2389 2393 - BceAI ACGGC 2374 2376 . . 2397 2400 + SetI ASST 2400 2396 . . 2398 2402 + HphI GGTGA 2410 2409 . . 2396 2399 - MnlI CCTC 2389 2388 . . 2403 2406 + SetI ASST 2406 2402 . . 2401 2405 - Hin4II* CCTTC 2395 2394 . . 2406 2410 - TspDTI ATGAA 2396 2394 . . 2422 2427 + Cac8I GCNNGC 2424 2424 . . 2428 2432 + HphI GGTGA 2440 2439 . . 2426 2429 - AciI CCGC 2426 2428 . . 2431 2435 + MboII GAAGA 2443 2442 . . 2453 2457 + Tsp4CI* ACNGT 2455 2454 . . 2456 2459 + Csp6I GTAC 2456 2458 . . 2456 2459 + RsaI GTAC 2457 2457 . . 2476 2481 + Eco57MI CTGRAG 2497 2495 . . 2476 2481 + Eco57I CTGAAG 2497 2495 . . 2478 2482 - Hin4II* CCTTC 2472 2471 . . 2488 2492 - FokI GGATG 2474 2478 . . 2488 2492 - BseGI GGATG 2487 2485 . . 2493 2498 + VspI ATTAAT 2494 2496 . . 2494 2497 + MseI TTAA 2494 2496 . . 2498 2502 + Hpy188I TCNGA 2500 2499 . . 2511 2516 + PsiI TTATAA 2513 2513 . . 2517 2522 + ApoI RAATTY 2517 2521 . . 2518 2521 + TspEI AATT 2517 2521 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 495 2037 # # AciI: # [CCGC] # 3 3 12 57 67 106 # 131 229 287 288 464 885 # # AcyI: # [GRCGYC] # 170 2362 # # AgeI: # [ACCGGT] # 1100 1432 # # AhaIII*: # [TTTAAA] # 153 2379 # # AjuI: # [GAANNNNNNNTTGG] # 32 734 1766 # # AloI: # [GAACNNNNNNTCC] # 32 1068 1432 # # AluI: # [AGCT] # 34 78 94 197 224 453 # 1452 # # AlwNI: # [CAGNNNCTG] # 97 2435 # # ApaI: # [GGGCCC] # 758 1774 # # ApoI: # [RAATTY] # 15 109 143 172 233 279 # 1581 # # AsuI*: # [GGNCC] # 1 365 389 839 938 # # AvaII: # [GGWCC] # 938 1594 # # BalI: # [TGGCCA] # 166 2366 # # BbvI: # [GCAGC] # 68 237 253 556 651 767 # # BccI: # [CCATC] # 95 376 542 1519 # # BceAI: # [ACGGC] # 63 158 856 1455 # # BcgI: # [CGANNNNNNTGC] # 34 208 2290 # # BciVI: # [GTATCC] # 156 405 1971 # # BdaI: # [TGANNNNNNTCA] # 0 0 0 0 34 34 # 497 919 1048 # # BetI*: # [WCCGGW] # 84 1100 1348 # # BinI*: # [GGATC] # 21 406 2105 # # BisI: # [GCNGC] # 33 68 220 223 230 315 # 651 792 # # BlsI: # [GCNGC] # 33 68 220 222 231 315 # 651 792 # # BmgT120I: # [GGNCC] # 1 365 390 839 937 # # BplI: # [GAGNNNNNCTC] # 0 0 0 0 2 2 # 30 421 2077 # # Bpu10I: # [CCTNAGC] # 198 260 2074 # # BsaBI: # [GATNNNNATC] # 153 773 1606 # # BseBI: # [CCWGG] # 387 2145 # # BseGI: # [GGATG] # 45 228 576 821 862 # # BseMII: # [CTCAG] # 472 627 1433 # # BseSI: # [GKGCMC] # 723 758 1051 # # BsgI: # [GTGCAG] # 780 850 902 # # BsiYI*: # [CCNNNNNNNGG] # 55 340 357 748 1032 # # BslFI: # [GGGAC] # 114 871 1547 # # BsmAI: # [GTCTC] # 106 118 138 155 229 330 # 375 530 551 # # BsmI: # [GAATGC] # 338 458 1736 # # Bsp120I: # [GGGCCC] # 754 1778 # # BspCNI: # [CTCAG] # 471 629 1432 # # BspHI: # [TCATGA] # 344 510 787 891 # # BspMI: # [ACCTGC] # 1159 1373 # # BsrBI: # [CCGCTC] # 906 1626 # # BsrDI: # [GCAATG] # 232 476 785 1039 # # BsrI: # [ACTGG] # 603 1929 # # BssKI: # [CCNGG] # 385 661 1486 # # BstAPI: # [GCANNNNNTGC] # 732 1800 # # BstEII: # [GGTNACC] # 471 2061 # # BstKTI: # [GATC] # 21 102 110 236 253 291 # 348 370 801 # # BtgZI: # [GCGATG] # 1035 1497 # # BtrI: # [CACGTC] # 151 2381 # # Cac8I: # [GCNNGC] # 108 220 289 348 654 913 # # CauII*: # [CCSGG] # 1048 1484 # # Cfr10I: # [RCCGGY] # 166 1100 1266 # # CfrI: # [YGGCCR] # 168 2364 # # ClaI: # [ATCGAT] # 110 376 512 547 987 # # Csp6I: # [GTAC] # 18 30 51 76 177 216 # 308 400 607 649 # # CviAII: # [CATG] # 23 33 39 52 66 69 # 132 151 172 174 209 649 # 763 # # CviJI: # [RGCY] # 17 23 27 29 33 34 # 46 51 58 61 67 77 # 82 99 101 110 143 149 # 197 1128 # # CviRI*: # [TGCA] # 15 57 63 103 157 176 # 182 200 202 252 360 371 # 394 # # DdeI: # [CTNAG] # 26 198 234 302 348 403 # 1021 # # DinI: # [GGCGCC] # 171 2361 # # DpnI: # [GATC] # 21 102 109 236 253 291 # 349 370 801 # # DraII: # [RGGNCCY] # 366 389 1777 # # DrdI: # [GACNNNNNNGTC] # 469 2063 # # DsaI*: # [CCRYGG] # 173 2359 # # EciI: # [GGCGGA] # 247 2285 # # Eco31I: # [GGTCTC] # 106 493 530 1403 # # Eco47III: # [AGCGCT] # 272 2260 # # Eco57I: # [CTGAAG] # 35 802 1695 # # Eco57MI: # [CTGRAG] # 35 354 448 1695 # # EcoP15I: # [CAGCAG] # 251 332 566 1383 # # EcoRII: # [CCWGG] # 385 2147 # # EcoRV: # [GATATC] # 576 1956 # # Esp3I: # [CGTCTC] # 229 330 1973 # # FatI: # [CATG] # 23 33 39 52 66 69 # 132 151 172 173 209 649 # 764 # # FauI: # [CCCGC] # 53 589 846 1044 # # FokI: # [GGATG] # 58 228 583 821 842 # # GlaI: # [GCGC] # 101 171 895 1365 # # GsuI: # [CTGGAG] # 448 2084 # # HaeII: # [RGCGCY] # 101 173 2258 # # HaeIII: # [GGCC] # 23 143 366 390 1610 # # HgaI: # [GACGC] # 191 265 2076 # # HgiAI*: # [GWGCWC] # 1137 1395 # # HgiCI*: # [GGYRCC] # 169 1056 1307 # # HgiJII*: # [GRGCYC] # 758 1774 # # HhaI: # [GCGC] # 101 172 895 1364 # # Hin4I: # [GAYNNNNNVTC] # 0 0 32 32 241 310 # 1917 # # Hin4II*: # [CCTTC] # 7 60 77 132 306 943 # 1007 # # Hin6I: # [GCGC] # 101 170 895 1366 # # HinfI: # [GANTC] # 11 24 60 61 73 123 # 150 219 300 344 384 783 # # HpaII: # [CCGG] # 84 167 301 881 1099 # # HphI: # [GGTGA] # 10 30 70 72 92 92 # 168 231 262 280 385 398 # 442 # # Hpy166II: # [GTNNAC] # 344 443 496 553 696 # # Hpy178III*: # [TCNNGA] # 13 26 31 33 46 49 # 51 64 78 98 105 242 # 252 267 287 402 488 # # Hpy188I: # [TCNGA] # 32 56 66 78 140 230 # 233 247 260 517 673 # # Hpy99I: # [CGWCG] # 490 2042 # # KasI: # [GGCGCC] # 169 2363 # # MaeI: # [CTAG] # 673 1859 # # MaeII: # [ACGT] # 8 11 131 180 267 1935 # # MaeIII: # [GTNAC] # 62 177 232 344 1717 # # MboI: # [GATC] # 21 102 107 236 253 291 # 351 370 801 # # MboII: # [GAAGA] # 3 3 7 63 89 99 # 129 201 232 442 543 721 # # MlyI: # [GAGTC] # 70 900 1562 # # MmeI: # [TCCRAC] # 450 542 755 785 # # MnlI: # [CCTC] # 34 42 43 73 75 131 # 134 143 261 279 333 464 # 520 # # MseI: # [TTAA] # 38 42 63 72 116 120 # 171 228 271 307 367 737 # # MslI: # [CAYNNNNRTG] # 509 2023 # # MwoI: # [GCNNNNNNNGC] # 100 262 278 354 528 1010 # # NarI: # [GGCGCC] # 170 2362 # # NcoI: # [CCATGG] # 173 2359 # # NdeI: # [CATATG] # 285 2247 # # NlaIII: # [CATG] # 23 33 39 52 66 69 # 132 151 172 177 209 649 # 760 # # NlaIV: # [GGNNCC] # 1 171 220 365 409 645 # 721 # # NspBII*: # [CMGCKG] # 229 282 318 1703 # # NspI: # [RCATGY] # 570 760 1202 # # PleI: # [GAGTC] # 69 900 1563 # # PsiI: # [TTATAA] # 19 2513 # # PvuII: # [CAGCTG] # 547 1985 # # RsaI: # [GTAC] # 18 30 51 75 177 216 # 308 400 608 649 # # ScrFI: # [CCNGG] # 387 661 1484 # # SduI: # [GDGCHC] # 344 379 758 1051 # # SecI*: # [CCNNGG] # 23 173 2336 # # SetI: # [ASST] # 4 6 8 8 9 11 # 11 12 14 16 22 34 # 34 42 46 69 74 78 # 81 90 99 115 126 180 # 271 331 345 396 # # SfaNI: # [GCATC] # 90 831 1611 # # SfeI*: # [CTRYAG] # 251 554 1727 # # SpeI: # [ACTAGT] # 672 1860 # # SphI: # [GCATGC] # 760 1772 # # StyI: # [CCWWGG] # 173 2359 # # SwaI: # [ATTTAAAT] # 153 2379 # # TaiI: # [ACGT] # 11 11 131 180 264 1935 # # TaqI: # [TCGA] # 110 214 333 376 512 987 # # TaqII: # [CACCCA] # 46 261 2225 # # TatI: # [WGTACW] # 18 30 216 385 400 700 # 783 # # TauI: # [GCSGC] # 232 1046 1254 # # TfiI: # [GAWTC] # 11 60 73 85 123 150 # 300 344 603 783 # # TseI: # [GCWGC] # 68 224 253 544 651 792 # # TsoI: # [TARCCA] # 992 1540 # # Tsp45I: # [GTSAC] # 62 409 2061 # # Tsp4CI*: # [ACNGT] # 77 246 415 716 1078 # # TspDTI: # [ATGAA] # 19 20 28 97 136 140 # 162 163 214 240 259 472 # 582 # # TspEI: # [AATT] # 7 8 10 13 15 25 # 32 40 47 57 66 84 # 86 102 142 172 177 229 # 270 950 # # TspGWI: # [ACGGA] # 19 30 152 247 271 293 # 298 471 751 # # VspI: # [ATTAAT] # 38 2494 # # XcmI: # [CCANNNNNNNNNTGG] # 1161 1371 # # XhoII: # [RGATCY] # 398 2134 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 2532 # Reported_sequences: 1 # Reported_hitcount: 575 #---------------------------------------