Restriction Fragments for NUP159/YIL115C



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The currently selected gene/sequence is :

NUP159/YIL115C on chromosome IX from coordinates 148709 to 144327.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.16073.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 15 19 + FokI GGATG 28 32 . . 15 19 + BseGI GGATG 21 19 . . 12 16 - Hin4II* CCTTC 6 5 . . 17 21 + TspDTI ATGAA 32 30 . . 22 25 + Csp6I GTAC 22 24 . . 22 25 + RsaI GTAC 23 23 . . 27 33 + TspRI CASTGNN 33 24 . . 29 33 + DdeI CTNAG 29 32 . . 29 33 - BspCNI CTCAG 21 19 . . 29 33 - BseMII CTCAG 20 18 . . 31 35 - BsmAI GTCTC 25 29 . . 37 41 + Hpy188I TCNGA 39 38 . . 40 44 + MboII GAAGA 52 51 . . 40 45 + BbvII* GAAGAC 47 51 . . 53 56 + MseI TTAA 53 55 . . 63 66 + SetI ASST 66 62 . . 74 79 + ApoI RAATTY 74 78 . . 75 78 + TspEI AATT 74 78 . . 81 84 + SetI ASST 84 80 . . 82 86 + Hin4II* CCTTC 92 91 . . 86 90 + Hin4II* CCTTC 96 95 . . 92 96 + TspDTI ATGAA 107 105 . . 108 111 + CviRI* TGCA 109 109 . . 109 113 + SfaNI GCATC 118 122 . . 123 128 + Hpy178III* TCNNGA 124 126 . . 125 128 + TaqI TCGA 125 127 . . 137 141 + Tsp4CI* ACNGT 139 138 . . 157 161 + BlsI GCNGC 159 158 . . 157 161 + BisI GCNGC 158 159 . . 157 161 + TseI GCWGC 157 160 . . 161 164 + MnlI CCTC 171 170 . . 157 161 - BbvI GCAGC 144 148 . . 175 178 - AciI CCGC 175 177 . . 188 191 + TspEI AATT 187 191 . . 194 197 + TspEI AATT 193 197 . . 199 203 + DdeI CTNAG 199 202 . . 199 203 - BspCNI CTCAG 191 189 . . 199 203 - BseMII CTCAG 190 188 . . 205 208 + DpnI GATC 206 206 . . 205 208 + BstKTI GATC 207 205 . . 205 208 + MboI GATC 204 208 . . 203 213 - Hin4I GAYNNNNNVTC 226 221 194 189 214 217 + SetI ASST 217 213 . . 215 218 + MnlI CCTC 225 224 . . 212 216 - HphI GGTGA 204 203 . . 217 222 + MmeI TCCRAC 242 240 . . 217 221 + Hpy188I TCNGA 219 218 . . 220 224 + HinfI GANTC 220 223 . . 220 224 - PleI GAGTC 214 215 . . 220 224 - MlyI GAGTC 214 214 . . 231 236 + Hpy166II GTNNAC 233 233 . . 231 236 + HpaI GTTAAC 233 233 . . 231 236 + HindII GTYRAC 233 233 . . 228 232 - TspGWI ACGGA 218 216 . . 232 235 + MseI TTAA 232 234 . . 234 239 + AclI AACGTT 235 237 . . 235 238 + TaiI ACGT 238 234 . . 235 238 + SetI ASST 238 234 . . 235 238 + MaeII ACGT 235 237 . . 267 272 + SspI AATATT 269 269 . . 283 286 + FatI CATG 282 286 . . 283 286 + NlaIII CATG 286 282 . . 283 286 + CviAII CATG 283 285 . . 281 285 - TspDTI ATGAA 271 269 . . 286 290 + HphI GGTGA 298 297 . . 288 293 + BclI TGATCA 288 292 . . 289 292 + DpnI GATC 290 290 . . 289 292 + BstKTI GATC 291 289 . . 289 292 + MboI GATC 288 292 . . 293 296 + SetI ASST 296 292 . . 314 319 + EcoT22I ATGCAT 318 314 . . 315 318 + CviRI* TGCA 316 316 . . 316 327 - BcgI CGANNNNNNTGC 339 337 305 303 337 340 - MnlI CCTC 330 329 . . 341 344 + TspEI AATT 340 344 . . 349 354 + ApoI RAATTY 349 353 . . 350 353 + TspEI AATT 349 353 . . 353 358 + AsuII TTCGAA 354 356 . . 354 357 + TaqI TCGA 354 356 . . 358 362 + Tsp4CI* ACNGT 360 359 . . 361 365 + Tsp45I GTSAC 360 365 . . 361 365 + MaeIII GTNAC 360 365 . . 377 380 + CviJI RGCY 378 378 . . 379 383 - BsrI ACTGG 379 377 . . 388 393 + MfeI CAATTG 388 392 . . 389 392 + TspEI AATT 388 392 . . 393 397 + MboII GAAGA 405 404 . . 397 402 + AclI AACGTT 398 400 . . 398 401 + TaiI ACGT 401 397 . . 398 401 + SetI ASST 401 397 . . 398 401 + MaeII ACGT 398 400 . . 401 404 + MseI TTAA 401 403 . . 417 420 + TspEI AATT 416 420 . . 420 425 + AhaIII* TTTAAA 422 422 . . 421 424 + MseI TTAA 421 423 . . 423 428 + ApoI RAATTY 423 427 . . 424 427 + TspEI AATT 423 427 . . 446 452 + TspRI CASTGNN 452 443 . . 447 451 + BsrI ACTGG 452 450 . . 454 457 + MseI TTAA 454 456 . . 466 469 + TaqI TCGA 466 468 . . 470 474 + DdeI CTNAG 470 473 . . 477 481 + BsrI ACTGG 482 480 . . 487 492 + AclI AACGTT 488 490 . . 488 491 + TaiI ACGT 491 487 . . 488 491 + SetI ASST 491 487 . . 488 491 + MaeII ACGT 488 490 . . 490 494 + MaeIII GTNAC 489 494 . . 493 496 + SetI ASST 496 492 . . 494 497 + MnlI CCTC 504 503 . . 505 509 + Tsp45I GTSAC 504 509 . . 505 509 + MaeIII GTNAC 504 509 . . 510 515 + ApoI RAATTY 510 514 . . 511 514 + TspEI AATT 510 514 . . 558 561 + CviRI* TGCA 559 559 . . 560 563 + FatI CATG 559 563 . . 560 563 + NlaIII CATG 563 559 . . 560 563 + CviAII CATG 560 562 . . 587 590 + TspEI AATT 586 590 . . 603 607 + Tsp4CI* ACNGT 605 604 . . 607 611 + Hpy188I TCNGA 609 608 . . 611 614 + TspEI AATT 610 614 . . 616 620 + MboII GAAGA 628 627 . . 616 625 + XmnI GAANNNNTTC 620 620 . . 620 623 + SetI ASST 623 619 . . 620 623 + CviJI RGCY 621 621 . . 620 623 + AluI AGCT 621 621 . . 616 621 - Ksp632I* CTCTTC 611 614 . . 616 622 - SapI GCTCTTC 611 614 . . 625 629 - BsrI ACTGG 625 623 . . 631 635 + MboII GAAGA 643 642 . . 635 640 + SspI AATATT 637 637 . . 652 656 + MaeIII GTNAC 651 656 . . 682 685 - AciI CCGC 682 684 . . 703 707 + Hpy188I TCNGA 705 704 . . 708 713 + TaqII GACCGA 724 722 . . 706 710 - BsmAI GTCTC 700 704 . . 706 711 - Eco31I GGTCTC 700 704 . . 723 727 - TspDTI ATGAA 713 711 . . 730 733 + DpnI GATC 731 731 . . 730 733 + BstKTI GATC 732 730 . . 730 733 + MboI GATC 729 733 . . 740 745 + TatI WGTACW 740 744 . . 740 745 + Bsp1407I TGTACA 740 744 . . 741 744 + Csp6I GTAC 741 743 . . 741 744 + RsaI GTAC 742 742 . . 746 751 + PsiI TTATAA 748 748 . . 761 765 + BceAI ACGGC 777 779 . . 764 768 + BlsI GCNGC 766 765 . . 764 768 + BisI GCNGC 765 766 . . 764 768 + BbvI GCAGC 776 780 . . 764 768 + TseI GCWGC 764 767 . . 766 771 + HaeII RGCGCY 770 766 . . 767 770 + Hin6I GCGC 767 769 . . 767 770 + GlaI GCGC 768 768 . . 767 770 + HhaI GCGC 769 767 . . 770 773 + MnlI CCTC 780 779 . . 777 782 + Hpy178III* TCNNGA 778 780 . . 798 801 + SetI ASST 801 797 . . 799 802 + MnlI CCTC 809 808 . . 799 809 + BsiYI* CCNNNNNNNGG 805 802 . . 820 823 + SetI ASST 823 819 . . 821 826 + NlaIV GGNNCC 823 823 . . 833 838 + TatI WGTACW 833 837 . . 833 838 + Bsp1407I TGTACA 833 837 . . 834 837 + Csp6I GTAC 834 836 . . 834 837 + RsaI GTAC 835 835 . . 841 845 + MaeIII GTNAC 840 845 . . 844 847 + SetI ASST 847 843 . . 856 859 + MseI TTAA 856 858 . . 858 861 + TspEI AATT 857 861 . . 864 867 + SetI ASST 867 863 . . 870 873 + CviRI* TGCA 871 871 . . 868 872 - SfaNI GCATC 858 862 . . 875 878 + TaiI ACGT 878 874 . . 875 878 + SetI ASST 878 874 . . 875 878 + MaeII ACGT 875 877 . . 885 890 + Cac8I GCNNGC 887 887 . . 885 890 + BmtI GCTAGC 889 885 . . 885 890 + NheI GCTAGC 885 889 . . 886 889 + MaeI CTAG 886 888 . . 889 893 + SfaNI GCATC 898 902 . . 890 899 + MslI CAYNNNNRTG 894 894 . . 896 899 + FatI CATG 895 899 . . 896 899 + NlaIII CATG 899 895 . . 896 899 + CviAII CATG 896 898 . . 920 924 + BslFI GGGAC 934 938 . . 922 926 + HinfI GANTC 922 925 . . 925 928 + TaqI TCGA 925 927 . . 922 926 - PleI GAGTC 916 917 . . 922 926 - MlyI GAGTC 916 916 . . 942 945 + SetI ASST 945 941 . . 943 947 + Hin4II* CCTTC 953 952 . . 947 952 + SecI* CCNNGG 947 951 . . 948 952 + BssKI CCNGG 947 952 . . 948 952 + BseBI CCWGG 949 950 . . 948 952 + ScrFI CCNGG 949 950 . . 948 952 + EcoRII CCWGG 947 952 . . 952 956 + TfiI GAWTC 952 955 . . 952 956 + HinfI GANTC 952 955 . . 955 959 + Hpy188I TCNGA 957 956 . . 974 978 + BccI CCATC 982 983 . . 978 984 + TspRI CASTGNN 984 975 . . 982 985 - MnlI CCTC 975 974 . . 1007 1018 + CspCI CAANNNNNGTGG 995 993 1030 1028 1011 1014 + SetI ASST 1014 1010 . . 1021 1026 + Hpy166II GTNNAC 1023 1023 . . 1021 1026 + HindII GTYRAC 1023 1023 . . 1024 1029 + ZraI GACGTC 1026 1026 . . 1024 1029 + AcyI GRCGYC 1025 1027 . . 1024 1029 + AatII GACGTC 1028 1024 . . 1025 1028 + TaiI ACGT 1028 1024 . . 1025 1028 + SetI ASST 1028 1024 . . 1025 1028 + MaeII ACGT 1025 1027 . . 1026 1030 + Hpy99I CGWCG 1030 1025 . . 1030 1034 + MaeIII GTNAC 1029 1034 . . 1034 1039 - Eco57MI CTGRAG 1019 1017 . . 1034 1039 - Eco57I CTGAAG 1019 1017 . . 1047 1052 + XbaI TCTAGA 1047 1051 . . 1047 1052 + Hpy178III* TCNNGA 1048 1050 . . 1048 1051 + MaeI CTAG 1048 1050 . . 1053 1056 + SetI ASST 1056 1052 . . 1054 1064 + BsiYI* CCNNNNNNNGG 1060 1057 . . 1060 1065 + BetI* WCCGGW 1060 1064 . . 1061 1064 + HpaII CCGG 1061 1063 . . 1080 1090 + MwoI GCNNNNNNNGC 1086 1083 . . 1082 1092 + Hin4I GAYNNNNNVTC 1073 1068 1105 1100 1086 1090 + TauI GCSGC 1089 1086 . . 1086 1090 + BlsI GCNGC 1088 1087 . . 1086 1090 + BisI GCNGC 1087 1088 . . 1087 1090 + AciI CCGC 1087 1089 . . 1087 1092 + BsrBI CCGCTC 1089 1089 . . 1093 1098 + Hpy166II GTNNAC 1095 1095 . . 1111 1123 + BarI GAAGNNNNNNTAC 1103 1098 1135 1130 1113 1116 + SetI ASST 1116 1112 . . 1111 1115 - Hin4II* CCTTC 1105 1104 . . 1117 1120 + SetI ASST 1120 1116 . . 1117 1120 + CviJI RGCY 1118 1118 . . 1117 1120 + AluI AGCT 1118 1118 . . 1141 1144 + FatI CATG 1140 1144 . . 1141 1144 + NlaIII CATG 1144 1140 . . 1141 1144 + CviAII CATG 1141 1143 . . 1139 1143 - TspDTI ATGAA 1129 1127 . . 1147 1151 + BlsI GCNGC 1149 1148 . . 1147 1151 + BisI GCNGC 1148 1149 . . 1147 1151 + BbvI GCAGC 1159 1163 . . 1147 1151 + TseI GCWGC 1147 1150 . . 1160 1164 + TauI GCSGC 1163 1160 . . 1160 1164 + BlsI GCNGC 1162 1161 . . 1160 1164 + BisI GCNGC 1161 1162 . . 1161 1166 + CfrI YGGCCR 1161 1165 . . 1162 1165 + CviJI RGCY 1163 1163 . . 1162 1165 + HaeIII GGCC 1163 1163 . . 1163 1173 + MwoI GCNNNNNNNGC 1169 1166 . . 1160 1163 - AciI CCGC 1160 1162 . . 1179 1184 + Hpy178III* TCNNGA 1180 1182 . . 1183 1187 + TfiI GAWTC 1183 1186 . . 1183 1187 + HinfI GANTC 1183 1186 . . 1195 1198 + FatI CATG 1194 1198 . . 1195 1198 + NlaIII CATG 1198 1194 . . 1195 1198 + CviAII CATG 1195 1197 . . 1219 1223 + Hpy188I TCNGA 1221 1220 . . 1222 1231 + XmnI GAANNNNTTC 1226 1226 . . 1226 1231 + ApoI RAATTY 1226 1230 . . 1227 1230 + TspEI AATT 1226 1230 . . 1231 1241 + BsiYI* CCNNNNNNNGG 1237 1234 . . 1252 1256 + MboII GAAGA 1264 1263 . . 1271 1275 + TspDTI ATGAA 1286 1284 . . 1273 1277 + TfiI GAWTC 1273 1276 . . 1273 1277 + HinfI GANTC 1273 1276 . . 1278 1288 + FalI AAGNNNNNCTT 1269 1264 1301 1296 1284 1287 + CviJI RGCY 1285 1285 . . 1278 1288 - FalI AAGNNNNNCTT 1301 1296 1269 1264 1291 1295 + Hpy188I TCNGA 1293 1292 . . 1296 1300 + TfiI GAWTC 1296 1299 . . 1296 1300 + HinfI GANTC 1296 1299 . . 1296 1305 + XmnI GAANNNNTTC 1300 1300 . . 1324 1327 + CviJI RGCY 1325 1325 . . 1337 1340 + MseI TTAA 1337 1339 . . 1349 1357 + AlwNI CAGNNNCTG 1354 1351 . . 1350 1353 + CviJI RGCY 1351 1351 . . 1351 1355 + TauI GCSGC 1354 1351 . . 1351 1355 + BlsI GCNGC 1353 1352 . . 1351 1355 + BisI GCNGC 1352 1353 . . 1352 1357 + NspBII* CMGCKG 1354 1354 . . 1352 1355 + AciI CCGC 1352 1354 . . 1354 1358 + BlsI GCNGC 1356 1355 . . 1354 1358 + BisI GCNGC 1355 1356 . . 1354 1358 + TseI GCWGC 1354 1357 . . 1354 1358 - BbvI GCAGC 1341 1345 . . 1363 1366 + CviJI RGCY 1364 1364 . . 1364 1369 + Cac8I GCNNGC 1366 1366 . . 1366 1371 + PstI CTGCAG 1370 1366 . . 1366 1371 + SfeI* CTRYAG 1366 1370 . . 1367 1370 + CviRI* TGCA 1368 1368 . . 1371 1375 + BsmAI GTCTC 1376 1380 . . 1381 1385 - MboII GAAGA 1373 1372 . . 1387 1390 + SetI ASST 1390 1386 . . 1394 1399 + SduI GDGCHC 1398 1394 . . 1394 1399 + HgiAI* GWGCWC 1398 1394 . . 1399 1409 + BsiYI* CCNNNNNNNGG 1405 1402 . . 1400 1403 + MnlI CCTC 1410 1409 . . 1408 1412 + BinI* GGATC 1416 1417 . . 1409 1412 + DpnI GATC 1410 1410 . . 1409 1412 + BstKTI GATC 1411 1409 . . 1409 1412 + MboI GATC 1408 1412 . . 1415 1418 + AciI CCGC 1415 1417 . . 1412 1416 - FokI GGATG 1398 1402 . . 1412 1416 - BseGI GGATG 1411 1409 . . 1420 1425 + AhaIII* TTTAAA 1422 1422 . . 1421 1424 + MseI TTAA 1421 1423 . . 1425 1428 + TspEI AATT 1424 1428 . . 1429 1439 + Hin4I GAYNNNNNVTC 1420 1415 1452 1447 1435 1439 + BccI CCATC 1443 1444 . . 1429 1439 - Hin4I GAYNNNNNVTC 1452 1447 1420 1415 1441 1445 + BsmAI GTCTC 1446 1450 . . 1453 1458 + TatI WGTACW 1453 1457 . . 1453 1458 + ScaI AGTACT 1455 1455 . . 1454 1457 + Csp6I GTAC 1454 1456 . . 1454 1457 + RsaI GTAC 1455 1455 . . 1451 1455 - BsrI ACTGG 1451 1449 . . 1456 1460 + BsrI ACTGG 1461 1459 . . 1465 1469 - HgaI GACGC 1454 1459 . . 1470 1476 + TspRI CASTGNN 1476 1467 . . 1472 1477 + Hpy166II GTNNAC 1474 1474 . . 1469 1473 - BsrI ACTGG 1469 1467 . . 1480 1484 + HgaI GACGC 1489 1494 . . 1487 1495 + AlwNI CAGNNNCTG 1492 1489 . . 1488 1493 + XhoII RGATCY 1488 1492 . . 1489 1492 + DpnI GATC 1490 1490 . . 1489 1492 + BstKTI GATC 1491 1489 . . 1489 1492 + MboI GATC 1488 1492 . . 1489 1493 - BinI* GGATC 1483 1484 . . 1501 1507 + EspI* GCTNAGC 1502 1505 . . 1502 1506 + DdeI CTNAG 1502 1505 . . 1505 1508 + CviJI RGCY 1506 1506 . . 1507 1517 + BsiYI* CCNNNNNNNGG 1513 1510 . . 1507 1511 - BsrI ACTGG 1507 1505 . . 1522 1526 + FauI CCCGC 1530 1532 . . 1522 1532 + BsiYI* CCNNNNNNNGG 1528 1525 . . 1523 1526 + AciI CCGC 1523 1525 . . 1524 1527 + FnuDII* CGCG 1525 1525 . . 1525 1535 + MwoI GCNNNNNNNGC 1531 1528 . . 1529 1533 + Hpy188I TCNGA 1531 1530 . . 1533 1536 + SetI ASST 1536 1532 . . 1533 1536 + CviJI RGCY 1534 1534 . . 1533 1536 + AluI AGCT 1534 1534 . . 1548 1552 + Tsp4CI* ACNGT 1550 1549 . . 1551 1556 + Hpy166II GTNNAC 1553 1553 . . 1551 1556 + HindII GTYRAC 1553 1553 . . 1558 1562 + Hpy188I TCNGA 1560 1559 . . 1561 1574 + AjuI GAANNNNNNNTTGG 1553 1548 1585 1580 1574 1579 + Cac8I GCNNGC 1576 1576 . . 1577 1580 + CviJI RGCY 1578 1578 . . 1579 1583 + BccI CCATC 1587 1588 . . 1579 1589 + BsiYI* CCNNNNNNNGG 1585 1582 . . 1579 1589 + PflMI CCANNNNNTGG 1585 1582 . . 1589 1594 + SduI GDGCHC 1593 1589 . . 1589 1594 + HgiAI* GWGCWC 1593 1589 . . 1594 1604 + BsiYI* CCNNNNNNNGG 1600 1597 . . 1595 1598 + MnlI CCTC 1605 1604 . . 1603 1606 + CviJI RGCY 1604 1604 . . 1606 1611 + Hpy178III* TCNNGA 1607 1609 . . 1624 1628 + TfiI GAWTC 1624 1627 . . 1624 1628 + HinfI GANTC 1624 1627 . . 1629 1634 + Cac8I GCNNGC 1631 1631 . . 1630 1640 + BsiYI* CCNNNNNNNGG 1636 1633 . . 1634 1637 + MnlI CCTC 1644 1643 . . 1636 1641 + Hpy178III* TCNNGA 1637 1639 . . 1636 1641 + BetI* WCCGGW 1636 1640 . . 1636 1641 + BspMII* TCCGGA 1636 1640 . . 1637 1640 + HpaII CCGG 1637 1639 . . 1639 1644 + XhoII RGATCY 1639 1643 . . 1639 1643 + BinI* GGATC 1647 1648 . . 1640 1643 + DpnI GATC 1641 1641 . . 1640 1643 + BstKTI GATC 1642 1640 . . 1640 1643 + MboI GATC 1639 1643 . . 1655 1658 + CviJI RGCY 1656 1656 . . 1657 1667 + BsiYI* CCNNNNNNNGG 1663 1660 . . 1658 1661 + MnlI CCTC 1668 1667 . . 1667 1670 + Csp6I GTAC 1667 1669 . . 1667 1670 + RsaI GTAC 1668 1668 . . 1672 1676 + Hin4II* CCTTC 1682 1681 . . 1672 1682 + BsiYI* CCNNNNNNNGG 1678 1675 . . 1681 1684 + CviJI RGCY 1682 1682 . . 1684 1689 + Hpy178III* TCNNGA 1685 1687 . . 1689 1694 + ApoI RAATTY 1689 1693 . . 1690 1693 + TspEI AATT 1689 1693 . . 1692 1696 - TspDTI ATGAA 1682 1680 . . 1703 1706 + CviJI RGCY 1704 1704 . . 1704 1708 + TauI GCSGC 1707 1704 . . 1704 1708 + BlsI GCNGC 1706 1705 . . 1704 1708 + BisI GCNGC 1705 1706 . . 1705 1708 + AciI CCGC 1705 1707 . . 1707 1712 + Cac8I GCNNGC 1709 1709 . . 1708 1718 + BsiYI* CCNNNNNNNGG 1714 1711 . . 1712 1715 + MnlI CCTC 1722 1721 . . 1714 1719 + Hpy178III* TCNNGA 1715 1717 . . 1714 1719 + BetI* WCCGGW 1714 1718 . . 1714 1719 + BspMII* TCCGGA 1714 1718 . . 1715 1718 + HpaII CCGG 1715 1717 . . 1717 1722 + XhoII RGATCY 1717 1721 . . 1717 1721 + BinI* GGATC 1725 1726 . . 1718 1721 + DpnI GATC 1719 1719 . . 1718 1721 + BstKTI GATC 1720 1718 . . 1718 1721 + MboI GATC 1717 1721 . . 1722 1725 + CviRI* TGCA 1723 1723 . . 1733 1736 + CviJI RGCY 1734 1734 . . 1735 1745 + BsiYI* CCNNNNNNNGG 1741 1738 . . 1736 1739 + MnlI CCTC 1746 1745 . . 1745 1748 + Csp6I GTAC 1745 1747 . . 1745 1748 + RsaI GTAC 1746 1746 . . 1750 1754 + Hin4II* CCTTC 1760 1759 . . 1750 1760 + BsiYI* CCNNNNNNNGG 1756 1753 . . 1759 1762 + CviJI RGCY 1760 1760 . . 1762 1767 + Hpy178III* TCNNGA 1763 1765 . . 1767 1772 + ApoI RAATTY 1767 1771 . . 1768 1771 + TspEI AATT 1767 1771 . . 1770 1774 - TspDTI ATGAA 1760 1758 . . 1777 1783 + EspI* GCTNAGC 1778 1781 . . 1778 1782 + DdeI CTNAG 1778 1781 . . 1775 1780 - EcoP15I CAGCAG 1747 1749 . . 1781 1784 + CviJI RGCY 1782 1782 . . 1782 1786 + TauI GCSGC 1785 1782 . . 1778 1782 - BspCNI CTCAG 1770 1768 . . 1778 1782 - BseMII CTCAG 1769 1767 . . 1782 1786 + BlsI GCNGC 1784 1783 . . 1782 1786 + BisI GCNGC 1783 1784 . . 1783 1786 + AciI CCGC 1783 1785 . . 1785 1790 + Cac8I GCNNGC 1787 1787 . . 1786 1796 + BsiYI* CCNNNNNNNGG 1792 1789 . . 1792 1797 + Hpy178III* TCNNGA 1793 1795 . . 1792 1797 + BetI* WCCGGW 1792 1796 . . 1792 1797 + BspMII* TCCGGA 1792 1796 . . 1793 1796 + HpaII CCGG 1793 1795 . . 1795 1800 + XhoII RGATCY 1795 1799 . . 1795 1799 + BinI* GGATC 1803 1804 . . 1796 1799 + DpnI GATC 1797 1797 . . 1796 1799 + BstKTI GATC 1798 1796 . . 1796 1799 + MboI GATC 1795 1799 . . 1811 1814 + CviJI RGCY 1812 1812 . . 1813 1823 + BsiYI* CCNNNNNNNGG 1819 1816 . . 1814 1817 + MnlI CCTC 1824 1823 . . 1823 1826 + Csp6I GTAC 1823 1825 . . 1823 1826 + RsaI GTAC 1824 1824 . . 1830 1833 + CviRI* TGCA 1831 1831 . . 1835 1839 + Hpy188I TCNGA 1837 1836 . . 1831 1836 - BsmI GAATGC 1831 1829 . . 1842 1845 + CviRI* TGCA 1843 1843 . . 1843 1847 + SfaNI GCATC 1852 1856 . . 1850 1854 + MaeIII GTNAC 1849 1854 . . 1864 1869 + Hpy178III* TCNNGA 1865 1867 . . 1867 1872 + XhoII RGATCY 1867 1871 . . 1867 1872 + NlaIV GGNNCC 1869 1869 . . 1867 1872 + BamHI GGATCC 1867 1871 . . 1867 1871 + BinI* GGATC 1875 1876 . . 1868 1871 + DpnI GATC 1869 1869 . . 1868 1871 + BstKTI GATC 1870 1868 . . 1868 1871 + MboI GATC 1867 1871 . . 1868 1872 - BinI* GGATC 1862 1863 . . 1884 1887 + CviJI RGCY 1885 1885 . . 1885 1889 + BlsI GCNGC 1887 1886 . . 1885 1889 + BisI GCNGC 1886 1887 . . 1885 1889 + TseI GCWGC 1885 1888 . . 1887 1890 + CviRI* TGCA 1888 1888 . . 1885 1889 - BbvI GCAGC 1872 1876 . . 1896 1900 - TspDTI ATGAA 1886 1884 . . 1908 1911 + CviRI* TGCA 1909 1909 . . 1912 1916 + FauI CCCGC 1920 1922 . . 1913 1916 + AciI CCGC 1913 1915 . . 1922 1926 + TspDTI ATGAA 1937 1935 . . 1931 1935 + Hpy188I TCNGA 1933 1932 . . 1933 1937 + BinI* GGATC 1941 1942 . . 1934 1937 + DpnI GATC 1935 1935 . . 1934 1937 + BstKTI GATC 1936 1934 . . 1934 1937 + MboI GATC 1933 1937 . . 1956 1959 + SetI ASST 1959 1955 . . 1958 1963 + SfeI* CTRYAG 1958 1962 . . 1960 1964 + Tsp4CI* ACNGT 1962 1961 . . 1963 1968 + Hpy166II GTNNAC 1965 1965 . . 1963 1968 + HindII GTYRAC 1965 1965 . . 1969 1972 + CviJI RGCY 1970 1970 . . 1971 1981 + BsiYI* CCNNNNNNNGG 1977 1974 . . 1979 1982 + SetI ASST 1982 1978 . . 1978 1981 - MnlI CCTC 1971 1970 . . 1984 1988 + TfiI GAWTC 1984 1987 . . 1984 1988 + HinfI GANTC 1984 1987 . . 1984 1994 + Hin4I GAYNNNNNVTC 1975 1970 2007 2002 1990 1993 + TaiI ACGT 1993 1989 . . 1990 1993 + SetI ASST 1993 1989 . . 1990 1993 + MaeII ACGT 1990 1992 . . 1992 1996 + Tsp45I GTSAC 1991 1996 . . 1992 1996 + MaeIII GTNAC 1991 1996 . . 1984 1994 - Hin4I GAYNNNNNVTC 2007 2002 1975 1970 1995 1998 + SetI ASST 1998 1994 . . 1993 1997 - HphI GGTGA 1985 1984 . . 2002 2006 + BccI CCATC 2010 2011 . . 2002 2012 + BsiYI* CCNNNNNNNGG 2008 2005 . . 2002 2012 + PflMI CCANNNNNTGG 2008 2005 . . 2005 2009 - MboII GAAGA 1997 1996 . . 2014 2017 + DpnI GATC 2015 2015 . . 2014 2017 + BstKTI GATC 2016 2014 . . 2014 2017 + MboI GATC 2013 2017 . . 2023 2027 + MboII GAAGA 2035 2034 . . 2029 2033 + PleI GAGTC 2037 2038 . . 2029 2033 + MlyI GAGTC 2038 2038 . . 2029 2033 + HinfI GANTC 2029 2032 . . 2033 2037 + DdeI CTNAG 2033 2036 . . 2041 2051 + Hin4I GAYNNNNNVTC 2032 2027 2064 2059 2044 2049 + Hpy166II GTNNAC 2046 2046 . . 2044 2049 + AccI GTMKAC 2045 2047 . . 2047 2051 + HinfI GANTC 2047 2050 . . 2049 2054 + Ksp632I* CTCTTC 2055 2058 . . 2047 2051 - PleI GAGTC 2041 2042 . . 2047 2051 - MlyI GAGTC 2041 2041 . . 2050 2054 - MboII GAAGA 2042 2041 . . 2056 2059 + TaqI TCGA 2056 2058 . . 2053 2057 - MboII GAAGA 2045 2044 . . 2061 2064 + SetI ASST 2064 2060 . . 2069 2072 + Csp6I GTAC 2069 2071 . . 2069 2072 + RsaI GTAC 2070 2070 . . 2076 2079 + SetI ASST 2079 2075 . . 2083 2086 + SetI ASST 2086 2082 . . 2084 2087 + MnlI CCTC 2094 2093 . . 2125 2129 - MboII GAAGA 2117 2116 . . 2134 2139 + XhoII RGATCY 2134 2138 . . 2134 2138 + BinI* GGATC 2142 2143 . . 2135 2138 + DpnI GATC 2136 2136 . . 2135 2138 + BstKTI GATC 2137 2135 . . 2135 2138 + MboI GATC 2134 2138 . . 2137 2142 + Hpy178III* TCNNGA 2138 2140 . . 2151 2154 + CviJI RGCY 2152 2152 . . 2154 2159 + Hpy178III* TCNNGA 2155 2157 . . 2158 2162 + TfiI GAWTC 2158 2161 . . 2158 2162 + HinfI GANTC 2158 2161 . . 2167 2172 + NlaIV GGNNCC 2169 2169 . . 2170 2174 + Hpy188I TCNGA 2172 2171 . . 2182 2187 + AhaIII* TTTAAA 2184 2184 . . 2183 2186 + MseI TTAA 2183 2185 . . 2185 2190 + ApoI RAATTY 2185 2189 . . 2186 2189 + TspEI AATT 2185 2189 . . 2192 2195 + Csp6I GTAC 2192 2194 . . 2192 2195 + RsaI GTAC 2193 2193 . . 2195 2199 + DdeI CTNAG 2195 2198 . . 2195 2199 + BspCNI CTCAG 2208 2206 . . 2195 2199 + BseMII CTCAG 2209 2207 . . 2199 2202 + CviJI RGCY 2200 2200 . . 2192 2204 - BarI GAAGNNNNNNTAC 2216 2211 2184 2179 2205 2208 + SetI ASST 2208 2204 . . 2203 2207 - HphI GGTGA 2195 2194 . . 2211 2216 + Ksp632I* CTCTTC 2217 2220 . . 2212 2216 - MboII GAAGA 2204 2203 . . 2217 2221 + Tsp4CI* ACNGT 2219 2218 . . 2233 2237 - HphI GGTGA 2225 2224 . . 2246 2250 + Hin4II* CCTTC 2256 2255 . . 2272 2277 + XhoII RGATCY 2272 2276 . . 2272 2276 + BinI* GGATC 2280 2281 . . 2273 2276 + DpnI GATC 2274 2274 . . 2273 2276 + BstKTI GATC 2275 2273 . . 2273 2276 + MboI GATC 2272 2276 . . 2278 2281 + MseI TTAA 2278 2280 . . 2286 2291 + SduI GDGCHC 2290 2286 . . 2286 2291 + HgiJII* GRGCYC 2290 2286 . . 2287 2290 + CviJI RGCY 2288 2288 . . 2295 2301 + TspRI CASTGNN 2301 2292 . . 2294 2298 - BsrI ACTGG 2294 2292 . . 2315 2319 + TspDTI ATGAA 2330 2328 . . 2317 2321 + MboII GAAGA 2329 2328 . . 2330 2335 + BsrDI GCAATG 2337 2335 . . 2335 2340 + NlaIV GGNNCC 2337 2337 . . 2335 2339 + BmgT120I GGNCC 2336 2337 . . 2335 2339 + AvaII GGWCC 2335 2338 . . 2335 2339 + AsuI* GGNCC 2335 2338 . . 2336 2340 - BslFI GGGAC 2321 2325 . . 2342 2345 + TaiI ACGT 2345 2341 . . 2342 2345 + SetI ASST 2345 2341 . . 2342 2345 + MaeII ACGT 2342 2344 . . 2348 2351 - AciI CCGC 2348 2350 . . 2365 2369 + TfiI GAWTC 2365 2368 . . 2365 2369 + HinfI GANTC 2365 2368 . . 2371 2375 + Tsp4CI* ACNGT 2373 2372 . . 2377 2387 - Hin4I GAYNNNNNVTC 2400 2395 2368 2363 2386 2390 - MboII GAAGA 2378 2377 . . 2393 2396 + MaeI CTAG 2393 2395 . . 2400 2405 + BetI* WCCGGW 2400 2404 . . 2401 2404 + HpaII CCGG 2401 2403 . . 2411 2414 + MnlI CCTC 2421 2420 . . 2411 2416 + SecI* CCNNGG 2411 2415 . . 2413 2417 + Hpy188I TCNGA 2415 2414 . . 2415 2419 + FokI GGATG 2428 2432 . . 2415 2419 + BseGI GGATG 2421 2419 . . 2417 2421 + TspDTI ATGAA 2432 2430 . . 2419 2423 + MboII GAAGA 2431 2430 . . 2425 2429 + HphI GGTGA 2437 2436 . . 2422 2426 - BccI CCATC 2416 2417 . . 2435 2438 + TaqI TCGA 2435 2437 . . 2437 2442 + BseRI GAGGAG 2452 2450 . . 2437 2440 - MnlI CCTC 2430 2429 . . 2440 2443 - MnlI CCTC 2433 2432 . . 2446 2449 + Hin6I GCGC 2446 2448 . . 2446 2449 + GlaI GCGC 2447 2447 . . 2446 2449 + HhaI GCGC 2448 2446 . . 2465 2470 + Cac8I GCNNGC 2467 2467 . . 2468 2471 + SetI ASST 2471 2467 . . 2468 2471 + CviJI RGCY 2469 2469 . . 2468 2471 + AluI AGCT 2469 2469 . . 2495 2509 + XcmI CCANNNNNNNNNTGG 2502 2501 . . 2497 2502 + SspI AATATT 2499 2499 . . 2505 2508 + FatI CATG 2504 2508 . . 2505 2508 + NlaIII CATG 2508 2504 . . 2505 2508 + CviAII CATG 2505 2507 . . 2508 2511 + CviJI RGCY 2509 2509 . . 2514 2519 + AhaIII* TTTAAA 2516 2516 . . 2515 2518 + MseI TTAA 2515 2517 . . 2524 2534 + BsiYI* CCNNNNNNNGG 2530 2527 . . 2539 2542 + FatI CATG 2538 2542 . . 2539 2542 + NlaIII CATG 2542 2538 . . 2539 2542 + CviAII CATG 2539 2541 . . 2554 2558 + Hpy188I TCNGA 2556 2555 . . 2557 2561 + TfiI GAWTC 2557 2560 . . 2557 2561 + HinfI GANTC 2557 2560 . . 2562 2566 - BceAI ACGGC 2547 2549 . . 2577 2580 + CviRI* TGCA 2578 2578 . . 2584 2589 + SspI AATATT 2586 2586 . . 2599 2602 + SetI ASST 2602 2598 . . 2599 2602 + CviJI RGCY 2600 2600 . . 2599 2602 + AluI AGCT 2600 2600 . . 2609 2615 + AarI CACCTGC 2619 2623 . . 2610 2613 + SetI ASST 2613 2609 . . 2610 2615 + BspMI ACCTGC 2619 2623 . . 2627 2631 + MaeIII GTNAC 2626 2631 . . 2638 2642 + TspDTI ATGAA 2653 2651 . . 2659 2663 + Tsp4CI* ACNGT 2661 2660 . . 2665 2669 - HphI GGTGA 2657 2656 . . 2674 2678 + MboII GAAGA 2686 2685 . . 2679 2682 + SetI ASST 2682 2678 . . 2679 2682 + CviJI RGCY 2680 2680 . . 2679 2682 + AluI AGCT 2680 2680 . . 2695 2700 + SpeI ACTAGT 2695 2699 . . 2696 2699 + MaeI CTAG 2696 2698 . . 2705 2711 + DraII RGGNCCY 2706 2709 . . 2706 2712 + DraII RGGNCCY 2707 2710 . . 2706 2711 + SduI GDGCHC 2710 2706 . . 2706 2711 + Bsp120I GGGCCC 2706 2710 . . 2706 2711 + NlaIV GGNNCC 2708 2708 . . 2706 2710 + BmgT120I GGNCC 2707 2708 . . 2706 2711 + HgiJII* GRGCYC 2710 2706 . . 2706 2711 + BseSI GKGCMC 2710 2706 . . 2706 2710 + AsuI* GGNCC 2706 2709 . . 2706 2711 + ApaI GGGCCC 2710 2706 . . 2707 2710 + CviJI RGCY 2708 2708 . . 2707 2710 + HaeIII GGCC 2708 2708 . . 2707 2712 + NlaIV GGNNCC 2709 2709 . . 2707 2711 + BmgT120I GGNCC 2708 2709 . . 2707 2711 + AsuI* GGNCC 2707 2710 . . 2716 2719 + SetI ASST 2719 2715 . . 2721 2725 + MboII GAAGA 2733 2732 . . 2721 2726 - Ksp632I* CTCTTC 2716 2719 . . 2733 2737 + TfiI GAWTC 2733 2736 . . 2733 2737 + HinfI GANTC 2733 2736 . . 2736 2740 - TspGWI ACGGA 2726 2724 . . 2730 2740 - BsaXI ACNNNNNCTCC 2752 2749 2722 2719 2755 2759 + MboII GAAGA 2767 2766 . . 2764 2768 + BsrI ACTGG 2769 2767 . . 2765 2770 + Eco57MI CTGRAG 2786 2784 . . 2765 2770 + GsuI CTGGAG 2786 2784 . . 2784 2788 + BinI* GGATC 2792 2793 . . 2785 2788 + DpnI GATC 2786 2786 . . 2785 2788 + BstKTI GATC 2787 2785 . . 2785 2788 + MboI GATC 2784 2788 . . 2788 2797 + MslI CAYNNNNRTG 2792 2792 . . 2800 2810 + BsiYI* CCNNNNNNNGG 2806 2803 . . 2800 2810 + PflMI CCANNNNNTGG 2806 2803 . . 2806 2810 - BccI CCATC 2800 2801 . . 2808 2813 - TsoI TARCCA 2798 2796 . . 2818 2823 + Hpy178III* TCNNGA 2819 2821 . . 2845 2849 + Hpy188I TCNGA 2847 2846 . . 2875 2879 + MboII GAAGA 2887 2886 . . 2875 2884 + XmnI GAANNNNTTC 2879 2879 . . 2879 2884 + ApoI RAATTY 2879 2883 . . 2880 2883 + TspEI AATT 2879 2883 . . 2889 2893 + Tsp45I GTSAC 2888 2893 . . 2889 2893 + MaeIII GTNAC 2888 2893 . . 2897 2900 + TaiI ACGT 2900 2896 . . 2897 2900 + SetI ASST 2900 2896 . . 2897 2900 + MaeII ACGT 2897 2899 . . 2899 2904 + Hpy166II GTNNAC 2901 2901 . . 2896 2901 - BtrI CACGTC 2898 2898 . . 2907 2912 + Hpy178III* TCNNGA 2908 2910 . . 2907 2912 + BspHI TCATGA 2907 2911 . . 2908 2911 + FatI CATG 2907 2911 . . 2908 2911 + NlaIII CATG 2911 2907 . . 2908 2911 + CviAII CATG 2908 2910 . . 2909 2913 + TspDTI ATGAA 2924 2922 . . 2939 2944 + Hpy166II GTNNAC 2941 2941 . . 2939 2944 + HindII GTYRAC 2941 2941 . . 2943 2948 + NspI RCATGY 2947 2943 . . 2944 2947 + FatI CATG 2943 2947 . . 2944 2947 + NlaIII CATG 2947 2943 . . 2944 2947 + CviAII CATG 2944 2946 . . 2955 2960 + BclI TGATCA 2955 2959 . . 2956 2959 + DpnI GATC 2957 2957 . . 2956 2959 + BstKTI GATC 2958 2956 . . 2956 2959 + MboI GATC 2955 2959 . . 2958 2963 + Hpy178III* TCNNGA 2959 2961 . . 2962 2972 + Hin4I GAYNNNNNVTC 2953 2948 2985 2980 2962 2972 - Hin4I GAYNNNNNVTC 2985 2980 2953 2948 2980 2984 + TspDTI ATGAA 2995 2993 . . 2984 2988 + TspDTI ATGAA 2999 2997 . . 2991 2996 + AsuII TTCGAA 2992 2994 . . 2992 2995 + TaqI TCGA 2992 2994 . . 2994 2998 + MboII GAAGA 3006 3005 . . 3002 3006 + TspDTI ATGAA 3017 3015 . . 3014 3019 + Hpy166II GTNNAC 3016 3016 . . 3014 3019 + HindII GTYRAC 3016 3016 . . 3011 3014 - AciI CCGC 3011 3013 . . 3009 3014 - BsrBI CCGCTC 3011 3011 . . 3019 3029 + BsiYI* CCNNNNNNNGG 3025 3022 . . 3019 3029 + PflMI CCANNNNNTGG 3025 3022 . . 3025 3028 + FatI CATG 3024 3028 . . 3025 3028 + NlaIII CATG 3028 3024 . . 3025 3028 + CviAII CATG 3025 3027 . . 3033 3044 + BstXI CCANNNNNNTGG 3040 3036 . . 3033 3038 + StyI CCWWGG 3033 3037 . . 3033 3038 + SecI* CCNNGG 3033 3037 . . 3040 3044 - BccI CCATC 3034 3035 . . 3065 3069 + TspDTI ATGAA 3080 3078 . . 3075 3080 + ApoI RAATTY 3075 3079 . . 3076 3079 + TspEI AATT 3075 3079 . . 3082 3086 + TfiI GAWTC 3082 3085 . . 3082 3086 + HinfI GANTC 3082 3085 . . 3104 3107 + TspEI AATT 3103 3107 . . 3106 3111 + AsuII TTCGAA 3107 3109 . . 3107 3110 + TaqI TCGA 3107 3109 . . 3109 3113 + MboII GAAGA 3121 3120 . . 3109 3114 + BbvII* GAAGAC 3116 3120 . . 3112 3122 + Hin4I GAYNNNNNVTC 3103 3098 3135 3130 3112 3122 - Hin4I GAYNNNNNVTC 3135 3130 3103 3098 3124 3128 + Hpy188I TCNGA 3126 3125 . . 3121 3125 - MboII GAAGA 3113 3112 . . 3127 3131 + MboII GAAGA 3139 3138 . . 3122 3127 - Eco57MI CTGRAG 3107 3105 . . 3122 3127 - Eco57I CTGAAG 3107 3105 . . 3127 3132 - Ksp632I* CTCTTC 3122 3125 . . 3130 3133 - MnlI CCTC 3123 3122 . . 3133 3137 + HgaI GACGC 3142 3147 . . 3135 3138 + FnuDII* CGCG 3136 3136 . . 3136 3141 + Cac8I GCNNGC 3138 3138 . . 3149 3153 + Tsp4CI* ACNGT 3151 3150 . . 3152 3157 + Hpy166II GTNNAC 3154 3154 . . 3152 3157 + AccI GTMKAC 3153 3155 . . 3161 3166 + BtsI GCAGTG 3168 3166 . . 3162 3168 + TspRI CASTGNN 3168 3159 . . 3170 3173 + MseI TTAA 3170 3172 . . 3175 3179 + TfiI GAWTC 3175 3178 . . 3175 3179 + HinfI GANTC 3175 3178 . . 3178 3182 + Hpy188I TCNGA 3180 3179 . . 3184 3194 + Hin4I GAYNNNNNVTC 3175 3170 3207 3202 3188 3193 + BspLU11I* ACATGT 3188 3192 . . 3188 3193 + NspI RCATGY 3192 3188 . . 3188 3193 + AflIII ACRYGT 3188 3192 . . 3189 3192 + FatI CATG 3188 3192 . . 3189 3192 + NlaIII CATG 3192 3188 . . 3189 3192 + CviAII CATG 3189 3191 . . 3192 3196 + Tsp45I GTSAC 3191 3196 . . 3192 3196 + MaeIII GTNAC 3191 3196 . . 3184 3194 - Hin4I GAYNNNNNVTC 3207 3202 3175 3170 3200 3204 + Tsp4CI* ACNGT 3202 3201 . . 3201 3207 + TspRI CASTGNN 3207 3198 . . 3203 3207 + Tsp45I GTSAC 3202 3207 . . 3203 3207 + MaeIII GTNAC 3202 3207 . . 3207 3211 + BssKI CCNGG 3206 3211 . . 3207 3210 + HpaII CCGG 3207 3209 . . 3207 3211 + ScrFI CCNGG 3208 3209 . . 3207 3211 + CauII* CCSGG 3208 3209 . . 3210 3214 + FokI GGATG 3223 3227 . . 3210 3214 + BseGI GGATG 3216 3214 . . 3212 3216 + TspDTI ATGAA 3227 3225 . . 3223 3227 + Hpy188I TCNGA 3225 3224 . . 3224 3228 + DdeI CTNAG 3224 3227 . . 3226 3230 + PleI GAGTC 3234 3235 . . 3226 3230 + MlyI GAGTC 3235 3235 . . 3226 3230 + HinfI GANTC 3226 3229 . . 3224 3228 - BspCNI CTCAG 3216 3214 . . 3224 3228 - BseMII CTCAG 3215 3213 . . 3240 3244 + BsrI ACTGG 3245 3243 . . 3244 3248 + MboII GAAGA 3256 3255 . . 3250 3255 + VspI ATTAAT 3251 3253 . . 3251 3254 + MseI TTAA 3251 3253 . . 3261 3272 + BdaI TGANNNNNNTCA 3250 3248 3284 3282 3263 3266 + SetI ASST 3266 3262 . . 3263 3266 + CviJI RGCY 3264 3264 . . 3263 3266 + AluI AGCT 3264 3264 . . 3267 3270 + SetI ASST 3270 3266 . . 3268 3271 + MnlI CCTC 3278 3277 . . 3268 3278 + BsiYI* CCNNNNNNNGG 3274 3271 . . 3271 3274 + FatI CATG 3270 3274 . . 3271 3274 + NlaIII CATG 3274 3270 . . 3271 3274 + CviAII CATG 3271 3273 . . 3261 3272 - BdaI TGANNNNNNTCA 3284 3282 3250 3248 3281 3286 + HindIII AAGCTT 3281 3285 . . 3282 3285 + SetI ASST 3285 3281 . . 3282 3285 + CviJI RGCY 3283 3283 . . 3282 3285 + AluI AGCT 3283 3283 . . 3283 3293 + MwoI GCNNNNNNNGC 3289 3286 . . 3286 3291 + AhaIII* TTTAAA 3288 3288 . . 3287 3290 + MseI TTAA 3287 3289 . . 3293 3298 + BsaAI YACGTR 3295 3295 . . 3293 3298 + PmaCI CACGTG 3295 3295 . . 3294 3297 + TaiI ACGT 3297 3293 . . 3294 3297 + SetI ASST 3297 3293 . . 3294 3297 + MaeII ACGT 3294 3296 . . 3304 3309 + HaeII RGCGCY 3308 3304 . . 3304 3309 + Eco47III AGCGCT 3306 3306 . . 3305 3308 + Hin6I GCGC 3305 3307 . . 3305 3308 + GlaI GCGC 3306 3306 . . 3305 3308 + HhaI GCGC 3307 3305 . . 3311 3316 + NspBII* CMGCKG 3313 3313 . . 3311 3314 + AciI CCGC 3311 3313 . . 3324 3329 + TatI WGTACW 3324 3328 . . 3324 3329 + Bsp1407I TGTACA 3324 3328 . . 3325 3328 + Csp6I GTAC 3325 3327 . . 3325 3328 + RsaI GTAC 3326 3326 . . 3333 3337 - TspDTI ATGAA 3323 3321 . . 3340 3344 + MboII GAAGA 3352 3351 . . 3340 3345 + BbvII* GAAGAC 3347 3351 . . 3346 3349 + TspEI AATT 3345 3349 . . 3355 3359 + TfiI GAWTC 3355 3358 . . 3355 3359 + HinfI GANTC 3355 3358 . . 3374 3377 + SetI ASST 3377 3373 . . 3379 3383 + Hpy188I TCNGA 3381 3380 . . 3389 3394 + StyI CCWWGG 3389 3393 . . 3389 3394 + SecI* CCNNGG 3389 3393 . . 3398 3402 + TspDTI ATGAA 3413 3411 . . 3409 3413 + TspGWI ACGGA 3424 3422 . . 3411 3415 + FokI GGATG 3424 3428 . . 3411 3415 + BseGI GGATG 3417 3415 . . 3412 3416 - SfaNI GCATC 3402 3406 . . 3417 3426 + MslI CAYNNNNRTG 3421 3421 . . 3434 3438 + Tsp4CI* ACNGT 3436 3435 . . 3436 3441 + TatI WGTACW 3436 3440 . . 3437 3442 + Hpy166II GTNNAC 3439 3439 . . 3437 3440 + Csp6I GTAC 3437 3439 . . 3437 3440 + RsaI GTAC 3438 3438 . . 3445 3449 + Tsp4CI* ACNGT 3447 3446 . . 3449 3452 + MseI TTAA 3449 3451 . . 3453 3457 - Hin4II* CCTTC 3447 3446 . . 3463 3468 + SalI GTCGAC 3463 3467 . . 3463 3468 + Hpy166II GTNNAC 3465 3465 . . 3463 3468 + HindII GTYRAC 3465 3465 . . 3463 3468 + AccI GTMKAC 3464 3466 . . 3464 3467 + TaqI TCGA 3464 3466 . . 3466 3475 + PshAI GACNNNNGTC 3470 3470 . . 3476 3479 + CviRI* TGCA 3477 3477 . . 3477 3487 + MwoI GCNNNNNNNGC 3483 3480 . . 3482 3486 + DdeI CTNAG 3482 3485 . . 3485 3488 + CviJI RGCY 3486 3486 . . 3482 3486 - BspCNI CTCAG 3474 3472 . . 3482 3486 - BseMII CTCAG 3473 3471 . . 3496 3501 + BspLU11I* ACATGT 3496 3500 . . 3496 3501 + NspI RCATGY 3500 3496 . . 3496 3501 + AflIII ACRYGT 3496 3500 . . 3497 3500 + FatI CATG 3496 3500 . . 3497 3500 + NlaIII CATG 3500 3496 . . 3497 3500 + CviAII CATG 3497 3499 . . 3502 3505 + TspEI AATT 3501 3505 . . 3525 3528 + SetI ASST 3528 3524 . . 3526 3530 + HphI GGTGA 3538 3537 . . 3527 3531 + Tsp45I GTSAC 3526 3531 . . 3527 3531 + MaeIII GTNAC 3526 3531 . . 3523 3527 - Hin4II* CCTTC 3517 3516 . . 3535 3538 + TspEI AATT 3534 3538 . . 3544 3555 + AlfI GCANNNNNNTGC 3533 3531 3567 3565 3549 3554 + BsrDI GCAATG 3556 3554 . . 3553 3556 + CviRI* TGCA 3554 3554 . . 3544 3555 - AlfI GCANNNNNNTGC 3567 3565 3533 3531 3565 3570 + MfeI CAATTG 3565 3569 . . 3566 3569 + TspEI AATT 3565 3569 . . 3575 3580 + SspI AATATT 3577 3577 . . 3588 3591 + CviRI* TGCA 3589 3589 . . 3601 3606 + SspI AATATT 3603 3603 . . 3621 3626 + ApoI RAATTY 3621 3625 . . 3622 3625 + TspEI AATT 3621 3625 . . 3628 3631 + TaiI ACGT 3631 3627 . . 3628 3631 + SetI ASST 3631 3627 . . 3628 3631 + MaeII ACGT 3628 3630 . . 3631 3634 + MseI TTAA 3631 3633 . . 3634 3637 + SetI ASST 3637 3633 . . 3646 3651 + TatI WGTACW 3646 3650 . . 3647 3650 + Csp6I GTAC 3647 3649 . . 3647 3650 + RsaI GTAC 3648 3648 . . 3654 3658 + Hpy188I TCNGA 3656 3655 . . 3658 3662 + Tsp4CI* ACNGT 3660 3659 . . 3659 3664 + McrI* CGRYCG 3662 3660 . . 3662 3665 + TaqI TCGA 3662 3664 . . 3666 3669 + SetI ASST 3669 3665 . . 3666 3669 + CviJI RGCY 3667 3667 . . 3666 3669 + AluI AGCT 3667 3667 . . 3668 3672 + DdeI CTNAG 3668 3671 . . 3672 3677 + Hpy166II GTNNAC 3674 3674 . . 3668 3672 - BspCNI CTCAG 3660 3658 . . 3668 3672 - BseMII CTCAG 3659 3657 . . 3676 3680 + Tsp4CI* ACNGT 3678 3677 . . 3681 3685 + Hpy188I TCNGA 3683 3682 . . 3684 3688 - BccI CCATC 3678 3679 . . 3695 3700 + Hpy178III* TCNNGA 3696 3698 . . 3695 3700 + BetI* WCCGGW 3695 3699 . . 3695 3700 + BspMII* TCCGGA 3695 3699 . . 3696 3699 + HpaII CCGG 3696 3698 . . 3696 3706 + BsiYI* CCNNNNNNNGG 3702 3699 . . 3693 3697 - FokI GGATG 3679 3683 . . 3693 3697 - BseGI GGATG 3692 3690 . . 3705 3710 - BciVI GTATCC 3699 3698 . . 3720 3725 + BclI TGATCA 3720 3724 . . 3721 3724 + DpnI GATC 3722 3722 . . 3721 3724 + BstKTI GATC 3723 3721 . . 3721 3724 + MboI GATC 3720 3724 . . 3727 3730 + TaqI TCGA 3727 3729 . . 3731 3735 + SfaNI GCATC 3740 3744 . . 3732 3736 - FokI GGATG 3718 3722 . . 3732 3736 - BseGI GGATG 3731 3729 . . 3741 3745 + HphI GGTGA 3753 3752 . . 3747 3757 + MwoI GCNNNNNNNGC 3753 3750 . . 3749 3752 + Csp6I GTAC 3749 3751 . . 3749 3752 + RsaI GTAC 3750 3750 . . 3751 3755 + Tsp4CI* ACNGT 3753 3752 . . 3752 3758 + TspRI CASTGNN 3758 3749 . . 3756 3759 - AciI CCGC 3756 3758 . . 3774 3779 + Hpy166II GTNNAC 3776 3776 . . 3774 3779 + BssNAI GTATAC 3776 3776 . . 3774 3779 + AccI GTMKAC 3775 3777 . . 3791 3799 + AlwNI CAGNNNCTG 3796 3793 . . 3795 3798 + CviJI RGCY 3796 3796 . . 3793 3796 - MnlI CCTC 3786 3785 . . 3800 3805 + ApoI RAATTY 3800 3804 . . 3801 3804 + TspEI AATT 3800 3804 . . 3808 3811 + MseI TTAA 3808 3810 . . 3811 3816 + SspI AATATT 3813 3813 . . 3816 3820 + Hpy188I TCNGA 3818 3817 . . 3833 3838 + Hpy166II GTNNAC 3835 3835 . . 3842 3845 + CviRI* TGCA 3843 3843 . . 3864 3869 + Hpy178III* TCNNGA 3865 3867 . . 3869 3872 + SetI ASST 3872 3868 . . 3871 3876 + Eco57MI CTGRAG 3892 3890 . . 3871 3876 + Eco57I CTGAAG 3892 3890 . . 3873 3877 - Hin4II* CCTTC 3867 3866 . . 3883 3887 + MaeIII GTNAC 3882 3887 . . 3896 3901 + Hpy178III* TCNNGA 3897 3899 . . 3909 3912 + CviRI* TGCA 3910 3910 . . 3920 3925 + Eco57MI CTGRAG 3941 3939 . . 3920 3925 + Eco57I CTGAAG 3941 3939 . . 3922 3926 + MboII GAAGA 3934 3933 . . 3929 3934 + TsoI TARCCA 3945 3943 . . 3930 3933 + CviJI RGCY 3931 3931 . . 3936 3939 + CviRI* TGCA 3937 3937 . . 3943 3948 + BseRI GAGGAG 3958 3956 . . 3943 3946 - MnlI CCTC 3936 3935 . . 3945 3955 - BsaXI ACNNNNNCTCC 3967 3964 3937 3934 3958 3961 + MseI TTAA 3958 3960 . . 3961 3966 + EcoT22I ATGCAT 3965 3961 . . 3962 3965 + CviRI* TGCA 3963 3963 . . 3964 3973 + MslI CAYNNNNRTG 3968 3968 . . 3973 3977 - SfaNI GCATC 3963 3967 . . 3979 3982 + TaqI TCGA 3979 3981 . . 3989 3992 + TaiI ACGT 3992 3988 . . 3989 3992 + SetI ASST 3992 3988 . . 3989 3992 + MaeII ACGT 3989 3991 . . 3985 3990 - BciVI GTATCC 3979 3978 . . 3992 3995 + MseI TTAA 3992 3994 . . 3996 4001 + XhoII RGATCY 3996 4000 . . 3996 4001 + BglII AGATCT 3996 4000 . . 3997 4000 + DpnI GATC 3998 3998 . . 3997 4000 + BstKTI GATC 3999 3997 . . 3997 4000 + MboI GATC 3996 4000 . . 4000 4004 + DdeI CTNAG 4000 4003 . . 4000 4004 + BspCNI CTCAG 4013 4011 . . 4000 4004 + BseMII CTCAG 4014 4012 . . 4004 4009 + Cac8I GCNNGC 4006 4006 . . 4008 4012 + SfaNI GCATC 4017 4021 . . 4013 4016 + TspEI AATT 4012 4016 . . 4019 4024 + BsmI GAATGC 4025 4023 . . 4022 4025 + CviRI* TGCA 4023 4023 . . 4029 4039 + Hin4I GAYNNNNNVTC 4020 4015 4052 4047 4029 4039 + Eam1105I GACNNNNNGTC 4034 4033 . . 4035 4038 + TaiI ACGT 4038 4034 . . 4035 4038 + SetI ASST 4038 4034 . . 4035 4038 + MaeII ACGT 4035 4037 . . 4029 4039 - Hin4I GAYNNNNNVTC 4052 4047 4020 4015 4043 4046 + SetI ASST 4046 4042 . . 4043 4046 + CviJI RGCY 4044 4044 . . 4043 4046 + AluI AGCT 4044 4044 . . 4049 4052 + TaqI TCGA 4049 4051 . . 4058 4061 + AciI CCGC 4058 4060 . . 4057 4062 - EciI GGCGGA 4047 4045 . . 4065 4068 + TspEI AATT 4064 4068 . . 4066 4071 + VspI ATTAAT 4067 4069 . . 4067 4070 + MseI TTAA 4067 4069 . . 4076 4081 + Eco57MI CTGRAG 4097 4095 . . 4076 4081 + Eco57I CTGAAG 4097 4095 . . 4078 4082 + MboII GAAGA 4090 4089 . . 4078 4083 - Ksp632I* CTCTTC 4073 4076 . . 4095 4100 + AhaIII* TTTAAA 4097 4097 . . 4096 4099 + MseI TTAA 4096 4098 . . 4100 4103 + TspEI AATT 4099 4103 . . 4104 4109 + Hpy166II GTNNAC 4106 4106 . . 4107 4113 + TspRI CASTGNN 4113 4104 . . 4108 4112 + Tsp4CI* ACNGT 4110 4109 . . 4116 4129 + AjuI GAANNNNNNNTTGG 4108 4103 4140 4135 4147 4157 + MwoI GCNNNNNNNGC 4153 4150 . . 4153 4156 + MaeI CTAG 4153 4155 . . 4155 4158 + SetI ASST 4158 4154 . . 4155 4158 + CviJI RGCY 4156 4156 . . 4155 4158 + AluI AGCT 4156 4156 . . 4160 4170 + FalI AAGNNNNNCTT 4151 4146 4183 4178 4162 4166 + TfiI GAWTC 4162 4165 . . 4162 4166 + HinfI GANTC 4162 4165 . . 4170 4173 + CviRI* TGCA 4171 4171 . . 4160 4170 - FalI AAGNNNNNCTT 4183 4178 4151 4146 4172 4177 + BsaAI YACGTR 4174 4174 . . 4172 4177 + PmaCI CACGTG 4174 4174 . . 4173 4176 + TaiI ACGT 4176 4172 . . 4173 4176 + SetI ASST 4176 4172 . . 4173 4176 + MaeII ACGT 4173 4175 . . 4175 4179 + Tsp45I GTSAC 4174 4179 . . 4175 4179 + MaeIII GTNAC 4174 4179 . . 4178 4182 + Tsp4CI* ACNGT 4180 4179 . . 4181 4186 + Hpy166II GTNNAC 4183 4183 . . 4199 4202 + TspEI AATT 4198 4202 . . 4222 4225 + SetI ASST 4225 4221 . . 4224 4228 + MaeIII GTNAC 4223 4228 . . 4228 4233 + MfeI CAATTG 4228 4232 . . 4229 4232 + TspEI AATT 4228 4232 . . 4234 4239 + BseRI GAGGAG 4249 4247 . . 4234 4237 - MnlI CCTC 4227 4226 . . 4242 4245 + SetI ASST 4245 4241 . . 4251 4254 + CviJI RGCY 4252 4252 . . 4256 4260 + Hpy99I CGWCG 4260 4255 . . 4244 4256 - BarI GAAGNNNNNNTAC 4268 4263 4236 4231 4262 4265 + TaqI TCGA 4262 4264 . . 4264 4274 + Hin4I GAYNNNNNVTC 4255 4250 4287 4282 4265 4270 + EcoT22I ATGCAT 4269 4265 . . 4266 4269 + CviRI* TGCA 4267 4267 . . 4267 4271 + SfaNI GCATC 4276 4280 . . 4264 4268 - SfaNI GCATC 4254 4258 . . 4271 4274 + MnlI CCTC 4281 4280 . . 4268 4272 - FokI GGATG 4254 4258 . . 4272 4277 + Ksp632I* CTCTTC 4278 4281 . . 4268 4272 - BseGI GGATG 4267 4265 . . 4273 4277 - MboII GAAGA 4265 4264 . . 4290 4293 + FatI CATG 4289 4293 . . 4290 4293 + NlaIII CATG 4293 4289 . . 4290 4293 + CviAII CATG 4290 4292 . . 4291 4295 + TspDTI ATGAA 4306 4304 . . 4300 4305 + AhaIII* TTTAAA 4302 4302 . . 4301 4304 + MseI TTAA 4301 4303 . . 4322 4327 + BalI TGGCCA 4324 4324 . . 4322 4327 + CfrI YGGCCR 4322 4326 . . 4323 4326 + CviJI RGCY 4324 4324 . . 4323 4326 + HaeIII GGCC 4324 4324 . . 4326 4329 + FatI CATG 4325 4329 . . 4326 4329 + NlaIII CATG 4329 4325 . . 4326 4329 + CviAII CATG 4326 4328 . . 4327 4331 + TspDTI ATGAA 4342 4340 . . 4344 4347 + TspEI AATT 4343 4347 . . 4348 4352 + HphI GGTGA 4360 4359 . . 4355 4359 - MboII GAAGA 4347 4346 . . 4362 4367 + ApoI RAATTY 4362 4366 . . 4363 4366 + TspEI AATT 4362 4366 . . 4371 4374 + FatI CATG 4370 4374 . . 4371 4374 + NlaIII CATG 4374 4370 . . 4371 4374 + CviAII CATG 4371 4373 . . #--------------------------------------- # # # AarI: # [CACCTGC] # 1764 2619 # # AatII: # [GACGTC] # 1028 3355 # # AccI: # [GTMKAC] # 311 311 608 1108 2045 # # AciI: # [CCGC] # 63 73 78 108 130 175 # 182 192 300 302 325 405 # 435 445 507 663 # # AclI: # [AACGTT] # 90 163 235 3895 # # AcyI: # [GRCGYC] # 1025 3358 # # AflIII: # [ACRYGT] # 308 887 3188 # # AhaIII*: # [TTTAAA] # 81 205 332 422 762 772 # 809 1000 # # AjuI: # [GAANNNNNNNTTGG] # 32 32 243 1553 2523 # # AlfI: # [GCANNNNNNTGC] # 0 0 34 816 3533 # # AluI: # [AGCT] # 19 80 112 131 227 377 # 384 416 497 584 621 935 # # AlwNI: # [CAGNNNCTG] # 138 587 1354 2304 # # ApaI: # [GGGCCC] # 1673 2710 # # ApoI: # [RAATTY] # 21 74 74 78 87 179 # 196 275 418 463 546 562 # 694 716 # # AsuI*: # [GGNCC] # 1 371 1676 2335 # # AsuII: # [TTCGAA] # 115 354 1276 2638 # # AvaII: # [GGWCC] # 2048 2335 # # BalI: # [TGGCCA] # 59 4324 # # BamHI: # [GGATCC] # 1867 2516 # # BarI: # [GAAGNNNNNNTAC] # 32 32 32 115 1049 1103 # 2020 # # BbvI: # [GCAGC] # 144 182 383 531 632 2511 # # BbvII*: # [GAAGAC] # 47 231 1036 3069 # # BccI: # [CCATC] # 144 234 384 406 423 461 # 644 705 982 # # BceAI: # [ACGGC] # 777 1770 1836 # # BcgI: # [CGANNNNNNTGC] # 34 305 4044 # # BciVI: # [GTATCC] # 280 404 3699 # # BclI: # [TGATCA] # 288 663 765 2667 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 1099 3250 # # BetI*: # [WCCGGW] # 78 78 576 608 688 1060 # 1295 # # BglII: # [AGATCT] # 387 3996 # # BinI*: # [GGATC] # 13 59 66 67 78 78 # 138 164 201 512 1416 1591 # # BisI: # [GCNGC] # 3 13 61 78 103 158 # 191 322 350 607 2497 # # BlsI: # [GCNGC] # 3 13 61 78 103 159 # 191 322 350 607 2496 # # BmgT120I: # [GGNCC] # 1 371 1675 2336 # # BmtI: # [GCTAGC] # 889 3494 # # BsaAI: # [YACGTR] # 209 879 3295 # # BsaXI: # [ACNNNNNCTCC] # 30 30 416 1185 2722 # # BseBI: # [CCWGG] # 949 3434 # # BseGI: # [GGATG] # 21 39 116 201 275 536 # 795 1010 1390 # # BseMII: # [CTCAG] # 20 170 186 258 355 369 # 440 1006 1579 # # BseRI: # [GAGGAG] # 134 291 1506 2452 # # BseSI: # [GKGCMC] # 1673 2710 # # BsiYI*: # [CCNNNNNNNGG] # 15 15 15 15 27 27 # 27 31 36 36 36 57 # 108 158 168 177 219 249 # 255 276 428 522 681 805 # # BslFI: # [GGGAC] # 934 1387 2062 # # BsmAI: # [GTCTC] # 25 70 675 676 2937 # # BsmI: # [GAATGC] # 358 1831 2194 # # Bsp120I: # [GGGCCC] # 1677 2706 # # Bsp1407I: # [TGTACA] # 93 740 1059 2491 # # BspCNI: # [CTCAG] # 21 170 186 258 353 370 # 438 1008 1579 # # BspHI: # [TCATGA] # 1476 2907 # # BspLU11I*: # [ACATGT] # 308 887 3188 # # BspMI: # [ACCTGC] # 1764 2619 # # BspMII*: # [TCCGGA] # 78 78 688 1636 1903 # # BsrBI: # [CCGCTC] # 1089 1372 1922 # # BsrDI: # [GCAATG] # 827 1219 2337 # # BsrI: # [ACTGG] # 8 10 30 38 73 143 # 379 475 476 787 826 1138 # # BssKI: # [CCNGG] # 947 1177 2259 # # BssNAI: # [GTATAC] # 607 3776 # # BstKTI: # [GATC] # 66 72 78 78 80 80 # 84 121 138 151 171 207 # 276 384 441 512 679 765 # # BstXI: # [CCANNNNNNTGG] # 1343 3040 # # BtrI: # [CACGTC] # 1485 2898 # # BtsI: # [GCAGTG] # 1215 3168 # # Cac8I: # [GCNNGC] # 55 78 78 210 377 479 # 671 680 868 887 # # CauII*: # [CCSGG] # 1175 3208 # # CfrI: # [YGGCCR] # 61 1161 3161 # # Csp6I: # [GTAC] # 22 78 78 93 102 112 # 123 210 213 246 620 634 # 719 1133 # # CspCI: # [CAANNNNNGTGG] # 35 995 3353 # # CviAII: # [CATG] # 12 34 36 36 45 54 # 81 82 164 226 245 277 # 283 336 369 793 1310 # # CviJI: # [RGCY] # 13 19 22 22 26 26 # 26 26 28 28 30 30 # 40 40 44 45 48 52 # 59 72 73 80 85 88 # 91 96 112 113 122 129 # 135 142 181 181 182 203 # 243 378 497 556 # # CviRI*: # [TGCA] # 12 21 26 27 35 45 # 60 67 77 96 108 109 # 116 148 207 243 254 312 # 355 497 669 899 # # DdeI: # [CTNAG] # 29 162 170 186 255 258 # 271 276 332 383 1029 1032 # # DpnI: # [GATC] # 66 72 78 78 80 80 # 84 121 138 151 171 206 # 276 385 441 512 679 765 # # DraII: # [RGGNCCY] # 1 1676 2706 # # Eam1105I: # [GACNNNNNGTC] # 349 4034 # # EciI: # [GGCGGA] # 336 4047 # # Eco31I: # [GGTCTC] # 700 3683 # # Eco47III: # [AGCGCT] # 1077 3306 # # Eco57I: # [CTGAAG] # 49 156 286 785 1019 2088 # # Eco57MI: # [CTGRAG] # 49 156 286 321 785 1019 # 1767 # # EcoP15I: # [CAGCAG] # 1747 2636 # # EcoRII: # [CCWGG] # 947 3436 # # EcoT22I: # [ATGCAT] # 114 304 318 3647 # # EspI*: # [GCTNAGC] # 276 1502 2605 # # FalI: # [AAGNNNNNCTT] # 0 0 0 0 32 32 # 200 1269 2850 # # FatI: # [CATG] # 13 34 36 36 45 54 # 81 82 164 226 245 277 # 282 336 369 793 1310 # # FauI: # [CCCGC] # 390 1530 2463 # # FnuDII*: # [CGCG] # 1247 1525 1611 # # FokI: # [GGATG] # 28 39 129 201 255 536 # 795 1030 1370 # # GlaI: # [GCGC] # 768 859 1077 1679 # # GsuI: # [CTGGAG] # 1597 2786 # # HaeII: # [RGCGCY] # 770 1075 2538 # # HaeIII: # [GGCC] # 59 1163 1545 1616 # # HgaI: # [GACGC] # 35 1241 1454 1653 # # HgiAI*: # [GWGCWC] # 195 1398 2790 # # HgiJII*: # [GRGCYC] # 420 1673 2290 # # HhaI: # [GCGC] # 769 859 1076 1679 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 0 0 # 0 0 0 0 0 0 # 25 32 32 32 32 32 # 32 32 32 32 32 32 # 40 96 118 194 203 304 # 315 523 553 813 847 # # Hin4II*: # [CCTTC] # 4 6 70 78 86 152 # 350 496 516 577 857 1191 # # Hin6I: # [GCGC] # 767 859 1078 1679 # # HindII: # [GTYRAC] # 75 233 412 449 530 790 # 918 976 # # HindIII: # [AAGCTT] # 1102 3281 # # HinfI: # [GANTC] # 18 23 30 45 51 90 # 93 111 129 176 192 207 # 220 221 231 328 349 360 # 702 807 # # HpaI: # [GTTAAC] # 233 4150 # # HpaII: # [CCGG] # 78 78 489 576 608 687 # 806 1061 # # HphI: # [GGTGA] # 23 30 94 204 210 212 # 215 220 607 881 1687 # # Hpy166II: # [GTNNAC] # 26 40 59 72 75 77 # 79 81 102 138 200 209 # 233 271 285 379 412 790 # 855 # # Hpy178III*: # [TCNNGA] # 17 30 30 30 32 48 # 48 51 72 89 124 132 # 169 270 273 427 486 654 # 664 737 # # Hpy188I: # [TCNGA] # 27 29 39 45 54 72 # 96 96 135 141 156 180 # 238 239 243 252 264 275 # 277 279 291 390 565 # # Hpy99I: # [CGWCG] # 123 1030 3230 # # Ksp632I*: # [CTCTTC] # 105 162 205 406 499 611 # 951 1444 # # MaeI: # [CTAG] # 162 230 303 886 1345 1457 # # MaeII: # [ACGT] # 46 90 138 150 163 210 # 235 334 352 361 387 397 # 555 965 # # MaeIII: # [GTNAC] # 11 15 49 129 142 147 # 160 189 189 262 292 303 # 324 356 360 635 820 # # MboI: # [GATC] # 66 72 78 78 80 80 # 84 121 138 151 171 204 # 276 387 441 512 679 765 # # MboII: # [GAAGA] # 3 7 8 15 18 34 # 36 38 47 49 52 53 # 72 82 87 96 107 109 # 117 119 120 125 156 175 # 223 255 353 582 621 624 # # McrI*: # [CGRYCG] # 721 3662 # # MfeI: # [CAATTG] # 155 388 663 3177 # # MlyI: # [GAGTC] # 3 214 702 1122 1148 1194 # # MmeI: # [TCCRAC] # 242 4141 # # MnlI: # [CCTC] # 3 9 24 24 29 39 # 54 54 54 78 102 105 # 123 147 150 155 166 171 # 174 195 276 291 327 435 # 508 690 # # MseI: # [TTAA] # 20 29 33 34 36 53 # 75 81 82 84 95 150 # 162 169 177 179 182 205 # 237 402 481 655 762 # # MslI: # [CAYNNNNRTG] # 415 547 629 894 1898 # # MwoI: # [GCNNNNNNNGC] # 83 194 230 270 362 400 # 1086 1758 # # NheI: # [GCTAGC] # 885 3498 # # NlaIII: # [CATG] # 9 34 36 36 45 54 # 81 82 164 226 245 277 # 286 336 369 793 1310 # # NlaIV: # [GGNNCC] # 1 168 300 371 823 1046 # 1674 # # NspBII*: # [CMGCKG] # 1070 1354 1959 # # NspI: # [RCATGY] # 245 308 883 2947 # # PflMI: # [CCANNNNNTGG] # 219 423 798 1358 1585 # # PleI: # [GAGTC] # 4 214 702 1121 1149 1193 # # PmaCI: # [CACGTG] # 209 879 3295 # # PshAI: # [GACNNNNGTC] # 913 3470 # # PsiI: # [TTATAA] # 748 3635 # # PstI: # [CTGCAG] # 1370 3013 # # RsaI: # [GTAC] # 23 78 78 93 102 112 # 123 210 213 246 620 633 # 719 1133 # # SalI: # [GTCGAC] # 920 3463 # # SapI: # [GCTCTTC] # 611 3772 # # ScaI: # [AGTACT] # 1455 2928 # # ScrFI: # [CCNGG] # 949 1175 2259 # # SduI: # [GDGCHC] # 195 420 697 1398 1673 # # SecI*: # [CCNNGG] # 356 622 947 994 1464 # # SetI: # [ASST] # 4 4 5 5 6 7 # 8 11 11 11 12 14 # 15 15 18 18 20 20 # 21 22 23 24 28 32 # 37 46 49 58 60 66 # 66 67 69 69 80 90 # 103 105 112 120 122 126 # 127 131 133 137 138 146 # 151 178 181 203 270 366 # 423 # # SfaNI: # [GCATC] # 22 40 54 107 118 223 # 237 338 740 954 1550 # # SfeI*: # [CTRYAG] # 592 1366 2425 # # SpeI: # [ACTAGT] # 1688 2695 # # SspI: # [AATATT] # 26 87 210 269 368 570 # 991 1862 # # StyI: # [CCWWGG] # 356 994 3033 # # TaiI: # [ACGT] # 46 90 138 150 163 207 # 238 334 352 361 387 397 # 555 965 # # TaqI: # [TCGA] # 65 70 112 115 121 125 # 198 213 229 252 357 379 # 459 557 1131 # # TaqII: # [GACCGA] # 724 3659 # # TatI: # [WGTACW] # 93 112 210 620 737 740 # 1871 # # TauI: # [GCSGC] # 74 78 191 353 1089 2598 # # TfiI: # [GAWTC] # 23 90 93 174 176 180 # 192 207 221 231 328 349 # 360 807 952 # # TseI: # [GCWGC] # 157 207 383 531 607 2498 # # TsoI: # [TARCCA] # 438 1147 2798 # # Tsp45I: # [GTSAC] # 11 144 209 303 324 360 # 648 897 1487 # # Tsp4CI*: # [ACNGT] # 11 18 51 70 75 139 # 154 203 213 221 234 245 # 257 288 357 412 490 945 # # TspDTI: # [ATGAA] # 4 18 32 36 41 51 # 63 71 75 78 90 96 # 102 126 147 157 164 221 # 271 393 396 416 442 893 # # TspEI: # [AATT] # 6 7 9 19 21 24 # 28 28 30 31 33 35 # 39 52 56 74 76 78 # 87 99 113 115 147 156 # 179 196 198 212 242 247 # 265 369 418 694 # # TspGWI: # [ACGGA] # 218 698 959 2508 # # TspRI: # [CASTGNN] # 33 39 270 355 419 492 # 532 551 825 867 # # VspI: # [ATTAAT] # 316 816 3251 # # XbaI: # [TCTAGA] # 1047 3336 # # XcmI: # [CCANNNNNNNNNTGG] # 1881 2502 # # XhoII: # [RGATCY] # 72 78 78 138 151 267 # 387 1488 1724 # # XmnI: # [GAANNNNTTC] # 74 606 620 1504 1579 # # ZraI: # [GACGTC] # 1026 3357 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 4383 # Reported_sequences: 1 # Reported_hitcount: 1071 #---------------------------------------