Restriction Fragments for YIL047C-A



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The currently selected gene/sequence is :

YIL047C-A on chromosome IX from coordinates 265467 to 265099.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.18397.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 1 5 + TspDTI ATGAA 16 14 . . 5 10 + BspLU11I* ACATGT 5 9 . . 5 10 + NspI RCATGY 9 5 . . 5 10 + AflIII ACRYGT 5 9 . . 6 9 + FatI CATG 5 9 . . 6 9 + NlaIII CATG 9 5 . . 6 9 + CviAII CATG 6 8 . . 18 23 + Hpy166II GTNNAC 20 20 . . 22 25 + SetI ASST 25 21 . . 23 33 + BsiYI* CCNNNNNNNGG 29 26 . . 20 24 - HphI GGTGA 12 11 . . 32 38 + BstEII GGTNACC 32 37 . . 33 37 + MaeIII GTNAC 32 37 . . 36 41 + Cfr10I RCCGGY 36 40 . . 36 41 + BetI* WCCGGW 36 40 . . 36 41 + AgeI ACCGGT 36 40 . . 37 40 + HpaII CCGG 37 39 . . 39 43 + HphI GGTGA 51 50 . . 40 44 + Tsp45I GTSAC 39 44 . . 40 44 + MaeIII GTNAC 39 44 . . 42 46 + HgaI GACGC 51 56 . . 48 51 + MnlI CCTC 58 57 . . 46 51 - BseRI GAGGAG 37 35 . . 61 66 + MfeI CAATTG 61 65 . . 62 65 + TspEI AATT 61 65 . . 65 70 + MstI* TGCGCA 67 67 . . 66 69 + Hin6I GCGC 66 68 . . 66 69 + GlaI GCGC 67 67 . . 66 69 + HhaI GCGC 68 66 . . 70 73 + SetI ASST 73 69 . . 68 73 - BspMI ACCTGC 59 63 . . 75 79 + HphI GGTGA 87 86 . . 81 85 + TfiI GAWTC 81 84 . . 81 85 + HinfI GANTC 81 84 . . 113 116 + CviJI RGCY 114 114 . . 119 123 - TspDTI ATGAA 109 107 . . 127 130 + MseI TTAA 127 129 . . 137 140 + SetI ASST 140 136 . . 147 151 + Hin4II* CCTTC 157 156 . . 153 156 + FatI CATG 152 156 . . 153 156 + NlaIII CATG 156 152 . . 153 156 + CviAII CATG 153 155 . . 165 168 + MnlI CCTC 175 174 . . 169 173 + Tsp4CI* ACNGT 171 170 . . 182 185 + SetI ASST 185 181 . . 188 193 + Cac8I GCNNGC 190 190 . . 189 193 + FauI CCCGC 197 199 . . 190 193 + AciI CCGC 190 192 . . 196 200 + Tsp4CI* ACNGT 198 197 . . 197 202 + DsaI* CCRYGG 197 201 . . 197 202 + SecI* CCNNGG 197 201 . . 201 205 + BslFI GGGAC 215 219 . . 208 212 + Hpy188I TCNGA 210 209 . . 211 215 + MboII GAAGA 223 222 . . 216 219 + SetI ASST 219 215 . . 216 222 + PpuMI RGGWCCY 217 220 . . 216 222 + DraII RGGNCCY 217 220 . . 217 221 + BmgT120I GGNCC 218 219 . . 217 221 + AvaII GGWCC 217 220 . . 217 221 + AsuI* GGNCC 217 220 . . 214 218 - Hin4II* CCTTC 208 207 . . 220 223 + MnlI CCTC 230 229 . . 222 226 - TspGWI ACGGA 212 210 . . 240 243 + TaqI TCGA 240 242 . . 240 245 + Hpy178III* TCNNGA 241 243 . . 243 248 + XhoII RGATCY 243 247 . . 244 247 + DpnI GATC 245 245 . . 244 247 + BstKTI GATC 246 244 . . 244 247 + MboI GATC 243 247 . . 247 257 + BsiYI* CCNNNNNNNGG 253 250 . . 247 257 + PflMI CCANNNNNTGG 253 250 . . 244 248 - BinI* GGATC 238 239 . . 252 257 + StyI CCWWGG 252 256 . . 252 257 + SecI* CCNNGG 252 256 . . 256 261 + SduI GDGCHC 260 256 . . 256 261 + BseSI GKGCMC 260 256 . . 260 265 + NspI RCATGY 264 260 . . 261 264 + FatI CATG 260 264 . . 261 264 + NlaIII CATG 264 260 . . 261 264 + CviAII CATG 261 263 . . 263 266 + CviRI* TGCA 264 264 . . 280 290 + Hin4I GAYNNNNNVTC 271 266 303 298 280 290 + Eam1105I GACNNNNNGTC 285 284 . . 285 289 + Tsp4CI* ACNGT 287 286 . . 280 290 - Hin4I GAYNNNNNVTC 303 298 271 266 294 298 + Tsp4CI* ACNGT 296 295 . . 295 300 + DsaI* CCRYGG 295 299 . . 295 300 + SecI* CCNNGG 295 299 . . 299 303 + FokI GGATG 312 316 . . 299 303 + BseGI GGATG 305 303 . . 300 310 + Hin4I GAYNNNNNVTC 291 286 323 318 306 309 + SetI ASST 309 305 . . 307 312 + Eco31I GGTCTC 313 317 . . 308 312 + BsmAI GTCTC 313 317 . . 300 310 - Hin4I GAYNNNNNVTC 323 318 291 286 311 316 + Hpy178III* TCNNGA 312 314 . . 358 361 + CviJI RGCY 359 359 . . 365 368 + FatI CATG 364 368 . . 365 368 + NlaIII CATG 368 364 . . 365 368 + CviAII CATG 365 367 . . #--------------------------------------- # # # AciI: # [CCGC] # 179 190 # # AflIII: # [ACRYGT] # 5 364 # # AgeI: # [ACCGGT] # 36 333 # # AsuI*: # [GGNCC] # 152 217 # # AvaII: # [GGWCC] # 152 217 # # BetI*: # [WCCGGW] # 36 333 # # BinI*: # [GGATC] # 131 238 # # BmgT120I: # [GGNCC] # 151 218 # # BseGI: # [GGATG] # 64 305 # # BseRI: # [GAGGAG] # 37 332 # # BseSI: # [GKGCMC] # 109 260 # # BsiYI*: # [CCNNNNNNNGG] # 29 116 224 # # BslFI: # [GGGAC] # 154 215 # # BsmAI: # [GTCTC] # 56 313 # # BspLU11I*: # [ACATGT] # 5 364 # # BspMI: # [ACCTGC] # 59 310 # # BstEII: # [GGTNACC] # 32 337 # # BstKTI: # [GATC] # 123 246 # # Cac8I: # [GCNNGC] # 179 190 # # Cfr10I: # [RCCGGY] # 36 333 # # CviAII: # [CATG] # 4 6 104 108 147 # # CviJI: # [RGCY] # 10 114 245 # # CviRI*: # [TGCA] # 105 264 # # DpnI: # [GATC] # 124 245 # # DraII: # [RGGNCCY] # 152 217 # # DsaI*: # [CCRYGG] # 74 98 197 # # Eam1105I: # [GACNNNNNGTC] # 84 285 # # Eco31I: # [GGTCTC] # 56 313 # # FatI: # [CATG] # 5 5 104 108 147 # # FauI: # [CCCGC] # 172 197 # # FokI: # [GGATG] # 57 312 # # GlaI: # [GCGC] # 67 302 # # HgaI: # [GACGC] # 51 318 # # HhaI: # [GCGC] # 68 301 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 12 20 # 20 46 271 # # Hin4II*: # [CCTTC] # 51 157 161 # # Hin6I: # [GCGC] # 66 303 # # HinfI: # [GANTC] # 81 288 # # HpaII: # [CCGG] # 37 332 # # HphI: # [GGTGA] # 12 36 39 282 # # Hpy166II: # [GTNNAC] # 20 349 # # Hpy178III*: # [TCNNGA] # 57 71 241 # # Hpy188I: # [TCNGA] # 159 210 # # MaeIII: # [GTNAC] # 7 32 330 # # MboI: # [GATC] # 126 243 # # MboII: # [GAAGA] # 146 223 # # MfeI: # [CAATTG] # 61 308 # # MnlI: # [CCTC] # 55 58 117 139 # # MseI: # [TTAA] # 127 242 # # MstI*: # [TGCGCA] # 67 302 # # NlaIII: # [CATG] # 1 9 104 108 147 # # NspI: # [RCATGY] # 9 105 255 # # PflMI: # [CCANNNNNTGG] # 116 253 # # PpuMI: # [RGGWCCY] # 152 217 # # SduI: # [GDGCHC] # 109 260 # # SecI*: # [CCNNGG] # 43 55 74 197 # # SetI: # [ASST] # 25 34 45 48 60 67 # 90 # # StyI: # [CCWWGG] # 117 252 # # TaqI: # [TCGA] # 129 240 # # TfiI: # [GAWTC] # 81 288 # # Tsp45I: # [GTSAC] # 39 330 # # Tsp4CI*: # [ACNGT] # 9 27 73 89 171 # # TspDTI: # [ATGAA] # 16 93 260 # # TspEI: # [AATT] # 61 308 # # TspGWI: # [ACGGA] # 157 212 # # XhoII: # [RGATCY] # 126 243 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 369 # Reported_sequences: 1 # Reported_hitcount: 100 #---------------------------------------