Restriction Fragments for BCY1/YIL033C



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The currently selected gene/sequence is :

BCY1/YIL033C on chromosome IX from coordinates 291669 to 290419.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.11030.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 17 22 + StyI CCWWGG 17 21 . . 17 22 + SecI* CCNNGG 17 21 . . 22 26 + TfiI GAWTC 22 25 . . 22 26 + HinfI GANTC 22 25 . . 27 32 + Cac8I GCNNGC 29 29 . . 30 33 + CviJI RGCY 31 31 . . 35 38 + TspEI AATT 34 38 . . 38 41 + CviRI* TGCA 39 39 . . 42 46 + Tsp4CI* ACNGT 44 43 . . 47 52 + Hpy178III* TCNNGA 48 50 . . 64 68 + TauI GCSGC 67 64 . . 64 68 + BlsI GCNGC 66 65 . . 64 68 + BisI GCNGC 65 66 . . 65 68 + AciI CCGC 65 67 . . 72 76 - TspGWI ACGGA 62 60 . . 76 81 + MmeI TCCRAC 101 99 . . 76 80 + Hpy188I TCNGA 78 77 . . 84 88 - MboII GAAGA 76 75 . . 85 90 - Eco57MI CTGRAG 70 68 . . 85 90 - Eco57I CTGAAG 70 68 . . 95 98 + AciI CCGC 95 97 . . 94 99 - EciI GGCGGA 84 82 . . 116 119 + CviJI RGCY 117 117 . . 131 141 + BplI GAGNNNNNCTC 122 117 154 149 138 141 + MnlI CCTC 148 147 . . 139 144 + SmlI CTYRAG 139 143 . . 131 141 - BplI GAGNNNNNCTC 154 149 122 117 144 147 + CviJI RGCY 145 145 . . 144 147 + HaeIII GGCC 145 145 . . 139 144 - Bce83I* CTTGAG 124 122 . . 146 150 + BssKI CCNGG 145 150 . . 146 150 + BseBI CCWGG 147 148 . . 146 151 + SecI* CCNNGG 146 150 . . 146 150 + ScrFI CCNGG 147 148 . . 146 150 + EcoRII CCWGG 145 150 . . 150 155 + NlaIV GGNNCC 152 152 . . 152 155 + CviJI RGCY 153 153 . . 157 162 + ApoI RAATTY 157 161 . . 158 161 + TspEI AATT 157 161 . . 161 164 + MseI TTAA 161 163 . . 186 191 + Hpy178III* TCNNGA 187 189 . . 186 191 + BetI* WCCGGW 186 190 . . 186 191 + BspMII* TCCGGA 186 190 . . 187 190 + HpaII CCGG 187 189 . . 189 194 + NlaIV GGNNCC 191 191 . . 196 201 + BseRI GAGGAG 211 209 . . 199 203 + PleI GAGTC 207 208 . . 196 199 - MnlI CCTC 189 188 . . 199 203 + MlyI GAGTC 208 208 . . 199 203 + HinfI GANTC 199 202 . . 208 213 + Hpy178III* TCNNGA 209 211 . . 213 216 + SetI ASST 216 212 . . 214 217 + MnlI CCTC 224 223 . . 222 225 + SetI ASST 225 221 . . 222 225 + CviJI RGCY 223 223 . . 222 225 + AluI AGCT 223 223 . . 235 240 + Hpy178III* TCNNGA 236 238 . . 238 243 + XhoII RGATCY 238 242 . . 239 242 + DpnI GATC 240 240 . . 239 242 + BstKTI GATC 241 239 . . 239 248 + BsaBI GATNNNNATC 243 243 . . 239 242 + MboI GATC 238 242 . . 241 246 + Hpy178III* TCNNGA 242 244 . . 239 243 - BinI* GGATC 233 234 . . 245 248 + DpnI GATC 246 246 . . 245 248 + BstKTI GATC 247 245 . . 245 248 + MboI GATC 244 248 . . 247 250 + TaqI TCGA 247 249 . . 274 279 + Hpy166II GTNNAC 276 276 . . 281 287 + PpuMI RGGWCCY 282 285 . . 281 287 + DraII RGGNCCY 282 285 . . 282 287 + NlaIV GGNNCC 284 284 . . 282 286 + BmgT120I GGNCC 283 284 . . 282 286 + AvaII GGWCC 282 285 . . 282 286 + AsuI* GGNCC 282 285 . . 280 283 - MnlI CCTC 273 272 . . 283 293 + Hin4I GAYNNNNNVTC 274 269 306 301 284 294 + BsaXI ACNNNNNCTCC 274 271 304 301 292 297 + MmeI TCCRAC 317 315 . . 296 299 + TaiI ACGT 299 295 . . 296 299 + SetI ASST 299 295 . . 296 299 + MaeII ACGT 296 298 . . 296 301 + AflIII ACRYGT 296 300 . . 301 306 + AhaIII* TTTAAA 303 303 . . 302 305 + MseI TTAA 302 304 . . 314 317 + MseI TTAA 314 316 . . 316 319 + TspEI AATT 315 319 . . 324 328 + FauI CCCGC 332 334 . . 325 328 + AciI CCGC 325 327 . . 342 346 - HphI GGTGA 334 333 . . 348 353 + Cac8I GCNNGC 350 350 . . 358 362 + MboII GAAGA 370 369 . . 374 377 + MaeI CTAG 374 376 . . 384 394 + Hin4I GAYNNNNNVTC 375 370 407 402 385 395 + BsaXI ACNNNNNCTCC 375 372 405 402 394 397 + MnlI CCTC 404 403 . . 392 397 - BseRI GAGGAG 383 381 . . 407 410 + CviRI* TGCA 408 408 . . 423 433 + FalI AAGNNNNNCTT 414 409 446 441 428 431 + Csp6I GTAC 428 430 . . 428 431 + RsaI GTAC 429 429 . . 423 433 - FalI AAGNNNNNCTT 446 441 414 409 442 446 + HphI GGTGA 454 453 . . 448 451 + SetI ASST 451 447 . . 445 449 - BsmAI GTCTC 439 443 . . 445 450 - Eco31I GGTCTC 439 443 . . 463 466 + TspEI AATT 462 466 . . 477 481 + HinfI GANTC 477 480 . . 477 487 + Hin4I GAYNNNNNVTC 468 463 500 495 480 485 + Hpy178III* TCNNGA 481 483 . . 477 481 - PleI GAGTC 471 472 . . 477 481 - MlyI GAGTC 471 471 . . 479 484 - Eco57MI CTGRAG 464 462 . . 484 487 + DpnI GATC 485 485 . . 484 487 + BstKTI GATC 486 484 . . 479 484 - GsuI CTGGAG 464 462 . . 484 487 + MboI GATC 483 487 . . 477 487 - Hin4I GAYNNNNNVTC 500 495 468 463 502 506 + Hpy188I TCNGA 504 503 . . 507 517 + MwoI GCNNNNNNNGC 513 510 . . 507 517 + BstAPI GCANNNNNTGC 513 510 . . 507 511 + BlsI GCNGC 509 508 . . 507 511 + BisI GCNGC 508 509 . . 507 511 + BbvI GCAGC 519 523 . . 507 511 + TseI GCWGC 507 510 . . 511 516 + MfeI CAATTG 511 515 . . 512 515 + TspEI AATT 511 515 . . 515 518 + CviRI* TGCA 516 516 . . 522 526 + BsrI ACTGG 527 525 . . 531 536 + ClaI ATCGAT 532 534 . . 532 535 + TaqI TCGA 532 534 . . 534 537 + DpnI GATC 535 535 . . 534 537 + BstKTI GATC 536 534 . . 534 537 + MboI GATC 533 537 . . 534 538 - BinI* GGATC 528 529 . . 536 540 - TspGWI ACGGA 526 524 . . 560 563 + SetI ASST 563 559 . . 560 563 + CviJI RGCY 561 561 . . 560 563 + AluI AGCT 561 561 . . 565 569 + TfiI GAWTC 565 568 . . 565 569 + HinfI GANTC 565 568 . . 565 575 + Hin4I GAYNNNNNVTC 556 551 588 583 568 573 + MmeI TCCRAC 593 591 . . 568 572 + Hpy188I TCNGA 570 569 . . 571 575 + HinfI GANTC 571 574 . . 571 575 - PleI GAGTC 565 566 . . 571 575 - MlyI GAGTC 565 565 . . 581 584 + CviJI RGCY 582 582 . . 592 595 + TspEI AATT 591 595 . . 597 602 + Hpy178III* TCNNGA 598 600 . . 598 603 + Eco57MI CTGRAG 619 617 . . 598 603 + GsuI CTGGAG 619 617 . . 601 606 + BseRI GAGGAG 616 614 . . 601 604 - MnlI CCTC 594 593 . . 600 610 - BsaXI ACNNNNNCTCC 622 619 592 589 601 611 - Hin4I GAYNNNNNVTC 624 619 592 587 610 614 - TspGWI ACGGA 600 598 . . 613 617 - BslFI GGGAC 598 602 . . 621 624 + SetI ASST 624 620 . . 642 645 + SetI ASST 645 641 . . 643 649 + BstEII GGTNACC 643 648 . . 643 647 + HphI GGTGA 655 654 . . 644 648 + Tsp45I GTSAC 643 648 . . 644 648 + MaeIII GTNAC 643 648 . . 648 653 + StyI CCWWGG 648 652 . . 648 653 + SecI* CCNNGG 648 652 . . 653 657 + BslFI GGGAC 667 671 . . 669 673 + Hpy99I CGWCG 673 668 . . 671 674 + TaqI TCGA 671 673 . . 680 683 + Csp6I GTAC 680 682 . . 680 683 + RsaI GTAC 681 681 . . 682 686 + Tsp4CI* ACNGT 684 683 . . 685 690 + Hpy166II GTNNAC 687 687 . . 685 690 + HindII GTYRAC 687 687 . . 688 699 + DrdI GACNNNNNNGTC 694 692 . . 695 698 + TaiI ACGT 698 694 . . 695 698 + SetI ASST 698 694 . . 695 698 + MaeII ACGT 695 697 . . 697 702 + Hpy166II GTNNAC 699 699 . . 697 702 + HindII GTYRAC 699 699 . . 703 714 + DrdI GACNNNNNNGTC 709 707 . . 710 713 + SetI ASST 713 709 . . 712 717 + Hpy166II GTNNAC 714 714 . . 712 717 + HindII GTYRAC 714 714 . . 717 722 + Ksp632I* CTCTTC 723 726 . . 718 722 - MboII GAAGA 710 709 . . 722 726 + BssKI CCNGG 721 726 . . 722 725 + HpaII CCGG 722 724 . . 722 726 + ScrFI CCNGG 723 724 . . 722 726 + CauII* CCSGG 723 724 . . 724 728 + BmgT120I GGNCC 725 726 . . 724 728 + AsuI* GGNCC 724 727 . . 725 728 + CviJI RGCY 726 726 . . 725 728 + HaeIII GGCC 726 726 . . 727 731 + BssKI CCNGG 726 731 . . 727 731 + BseBI CCWGG 728 729 . . 727 731 + ScrFI CCNGG 728 729 . . 727 731 + EcoRII CCWGG 726 731 . . 730 733 + CviJI RGCY 731 731 . . 730 735 + NlaIV GGNNCC 732 732 . . 734 744 + BsiYI* CCNNNNNNNGG 740 737 . . 732 737 - Eco57MI CTGRAG 717 715 . . 732 737 - GsuI CTGGAG 717 715 . . 734 738 - BsrI ACTGG 734 732 . . 758 763 + TatI WGTACW 758 762 . . 758 763 + Bsp1407I TGTACA 758 762 . . 759 762 + Csp6I GTAC 759 761 . . 759 762 + RsaI GTAC 760 760 . . 766 769 + CviJI RGCY 767 767 . . 769 772 + MnlI CCTC 779 778 . . 775 779 + BlsI GCNGC 777 776 . . 775 779 + BisI GCNGC 776 777 . . 770 775 - BsiI* CACGAG 770 774 . . 775 779 + TseI GCWGC 775 778 . . 775 779 - BbvI GCAGC 762 766 . . 781 785 + Tsp4CI* ACNGT 783 782 . . 793 796 + SetI ASST 796 792 . . 794 797 + MnlI CCTC 804 803 . . 796 801 + MmeI TCCRAC 821 819 . . 796 800 + Hpy188I TCNGA 798 797 . . 800 804 + Tsp4CI* ACNGT 802 801 . . 812 817 + SduI GDGCHC 816 812 . . 812 817 + HgiJII* GRGCYC 816 812 . . 813 816 + CviJI RGCY 814 814 . . 816 821 + XbaI TCTAGA 816 820 . . 816 821 + Hpy178III* TCNNGA 817 819 . . 817 820 + MaeI CTAG 817 819 . . 824 827 + CviJI RGCY 825 825 . . 829 832 + SetI ASST 832 828 . . 830 834 + Hin4II* CCTTC 840 839 . . 827 831 - HphI GGTGA 819 818 . . 833 837 + Hpy188I TCNGA 835 834 . . 831 836 - Eco57MI CTGRAG 816 814 . . 831 836 - Eco57I CTGAAG 816 814 . . 851 855 + BlsI GCNGC 853 852 . . 851 855 + BisI GCNGC 852 853 . . 851 855 + BbvI GCAGC 863 867 . . 851 855 + TseI GCWGC 851 854 . . 853 856 + SetI ASST 856 852 . . 853 856 + CviJI RGCY 854 854 . . 853 856 + AluI AGCT 854 854 . . 860 865 + Hpy178III* TCNNGA 861 863 . . 864 868 + MboII GAAGA 876 875 . . 869 873 + HinfI GANTC 869 872 . . 864 869 - Ksp632I* CTCTTC 859 862 . . 867 871 - BsmAI GTCTC 861 865 . . 869 873 - PleI GAGTC 863 864 . . 869 873 - MlyI GAGTC 863 863 . . 873 876 + FatI CATG 872 876 . . 873 876 + NlaIII CATG 876 872 . . 873 876 + CviAII CATG 873 875 . . 883 886 + DpnI GATC 884 884 . . 883 886 + BstKTI GATC 885 883 . . 883 886 + MboI GATC 882 886 . . 891 895 + MboII GAAGA 903 902 . . 896 901 + NspI RCATGY 900 896 . . 896 901 + Cac8I GCNNGC 898 898 . . 891 896 - Ksp632I* CTCTTC 886 889 . . 896 901 + SphI GCATGC 900 896 . . 897 900 + FatI CATG 896 900 . . 897 900 + NlaIII CATG 900 896 . . 897 900 + CviAII CATG 897 899 . . 891 897 - SapI GCTCTTC 886 889 . . 901 905 - BsrI ACTGG 901 899 . . 909 913 + MboII GAAGA 921 920 . . 909 914 - Ksp632I* CTCTTC 904 907 . . 921 926 + SnaBI TACGTA 923 923 . . 921 926 + BsaAI YACGTR 923 923 . . 922 925 + TaiI ACGT 925 921 . . 922 925 + SetI ASST 925 921 . . 922 925 + MaeII ACGT 922 924 . . 923 928 + SplI* CGTACG 923 927 . . 924 927 + Csp6I GTAC 924 926 . . 924 927 + RsaI GTAC 925 925 . . 927 932 + McrI* CGRYCG 930 928 . . 929 933 + Tsp4CI* ACNGT 931 930 . . 934 944 + MwoI GCNNNNNNNGC 940 937 . . 948 951 + CviRI* TGCA 949 949 . . 950 956 + TspRI CASTGNN 956 947 . . 946 950 - SfaNI GCATC 936 940 . . 951 955 + BsrI ACTGG 956 954 . . 954 959 - BciVI GTATCC 948 947 . . 962 967 + XhoII RGATCY 962 966 . . 962 967 + BglII AGATCT 962 966 . . 963 966 + DpnI GATC 964 964 . . 963 966 + BstKTI GATC 965 963 . . 963 966 + MboI GATC 962 966 . . 971 974 + CviJI RGCY 972 972 . . 973 977 + BssKI CCNGG 972 977 . . 973 976 + HpaII CCGG 973 975 . . 973 977 + ScrFI CCNGG 974 975 . . 973 977 + CauII* CCSGG 974 975 . . 976 980 + HphI GGTGA 988 987 . . 990 995 + Hpy178III* TCNNGA 991 993 . . 990 995 + NruI TCGCGA 992 992 . . 991 994 + FnuDII* CGCG 992 992 . . 994 997 - MnlI CCTC 987 986 . . 997 1001 + HphI GGTGA 1009 1008 . . 999 1004 + BclI TGATCA 999 1003 . . 1000 1003 + DpnI GATC 1001 1001 . . 1000 1003 + BstKTI GATC 1002 1000 . . 1000 1003 + MboI GATC 999 1003 . . 1021 1024 + MseI TTAA 1021 1023 . . 1023 1026 + TspEI AATT 1022 1026 . . 1029 1032 + Csp6I GTAC 1029 1031 . . 1029 1032 + RsaI GTAC 1030 1030 . . 1031 1035 + TspGWI ACGGA 1046 1044 . . 1035 1038 + SetI ASST 1038 1034 . . 1035 1038 + CviJI RGCY 1036 1036 . . 1035 1038 + AluI AGCT 1036 1036 . . 1039 1044 + Hpy166II GTNNAC 1041 1041 . . 1042 1047 + ZraI GACGTC 1044 1044 . . 1042 1047 + AcyI GRCGYC 1043 1045 . . 1042 1047 + AatII GACGTC 1046 1042 . . 1043 1046 + TaiI ACGT 1046 1042 . . 1043 1046 + SetI ASST 1046 1042 . . 1043 1046 + MaeII ACGT 1043 1045 . . 1044 1049 + Esp3I CGTCTC 1050 1054 . . 1045 1049 + BsmAI GTCTC 1050 1054 . . 1049 1053 + DdeI CTNAG 1049 1052 . . 1056 1060 + BmgT120I GGNCC 1057 1058 . . 1056 1060 + AsuI* GGNCC 1056 1059 . . 1053 1057 - Hin4II* CCTTC 1047 1046 . . 1057 1060 + CviJI RGCY 1058 1058 . . 1057 1060 + HaeIII GGCC 1058 1058 . . 1059 1064 + StyI CCWWGG 1059 1063 . . 1059 1064 + SecI* CCNNGG 1059 1063 . . 1062 1065 + SetI ASST 1065 1061 . . 1087 1090 + FatI CATG 1086 1090 . . 1087 1090 + NlaIII CATG 1090 1086 . . 1087 1090 + CviAII CATG 1087 1089 . . 1099 1103 + HphI GGTGA 1111 1110 . . 1107 1110 + CviJI RGCY 1108 1108 . . 1107 1110 + HaeIII GGCC 1108 1108 . . 1134 1137 + CviJI RGCY 1135 1135 . . 1134 1137 + HaeIII GGCC 1135 1135 . . 1137 1143 + TspRI CASTGNN 1143 1134 . . 1138 1142 + Tsp4CI* ACNGT 1140 1139 . . 1141 1145 + Tsp45I GTSAC 1140 1145 . . 1141 1145 + MaeIII GTNAC 1140 1145 . . 1169 1179 + BsiYI* CCNNNNNNNGG 1175 1172 . . 1193 1198 + AclI AACGTT 1194 1196 . . 1194 1197 + TaiI ACGT 1197 1193 . . 1194 1197 + SetI ASST 1197 1193 . . 1194 1197 + MaeII ACGT 1194 1196 . . 1196 1201 + Hpy166II GTNNAC 1198 1198 . . 1200 1204 + BsrI ACTGG 1205 1203 . . 1200 1205 + BfiI ACTGGG 1210 1209 . . 1203 1209 + PpuMI RGGWCCY 1204 1207 . . 1203 1209 + DraII RGGNCCY 1204 1207 . . 1203 1208 + NlaIV GGNNCC 1205 1205 . . 1204 1208 + BmgT120I GGNCC 1205 1206 . . 1204 1208 + AvaII GGWCC 1204 1207 . . 1204 1208 + AsuI* GGNCC 1204 1207 . . 1208 1213 + PstI CTGCAG 1212 1208 . . 1208 1213 + SfeI* CTRYAG 1208 1212 . . 1209 1212 + CviRI* TGCA 1210 1210 . . 1213 1218 + Hpy166II GTNNAC 1215 1215 . . 1213 1218 + AccI GTMKAC 1214 1216 . . 1217 1220 + TaiI ACGT 1220 1216 . . 1217 1220 + SetI ASST 1220 1216 . . 1217 1220 + MaeII ACGT 1217 1219 . . 1222 1225 + MseI TTAA 1222 1224 . . 1226 1229 + SetI ASST 1229 1225 . . 1226 1229 + CviJI RGCY 1227 1227 . . 1226 1229 + AluI AGCT 1227 1227 . . 1234 1237 + DpnI GATC 1235 1235 . . 1234 1237 + BstKTI GATC 1236 1234 . . 1234 1237 + MboI GATC 1233 1237 . . 1234 1238 - BinI* GGATC 1228 1229 . . 1248 1251 + MseI TTAA 1248 1250 . . #--------------------------------------- # # # AatII: # [GACGTC] # 205 1046 # # AccI: # [GTMKAC] # 37 1214 # # AciI: # [CCGC] # 30 65 230 926 # # AclI: # [AACGTT] # 57 1194 # # AcyI: # [GRCGYC] # 208 1043 # # AflIII: # [ACRYGT] # 296 955 # # AhaIII*: # [TTTAAA] # 303 948 # # AluI: # [AGCT] # 24 182 191 223 293 338 # # ApoI: # [RAATTY] # 157 1094 # # AsuI*: # [GGNCC] # 47 148 282 332 442 # # AvaII: # [GGWCC] # 47 282 922 # # BbvI: # [GCAGC] # 101 243 388 519 # # Bce83I*: # [CTTGAG] # 124 1127 # # BciVI: # [GTATCC] # 303 948 # # BclI: # [TGATCA] # 252 999 # # BetI*: # [WCCGGW] # 186 1065 # # BfiI: # [ACTGGG] # 41 1210 # # BglII: # [AGATCT] # 289 962 # # BinI*: # [GGATC] # 23 233 295 700 # # BisI: # [GCNGC] # 65 76 268 399 443 # # BlsI: # [GCNGC] # 66 76 268 398 443 # # BmgT120I: # [GGNCC] # 46 148 283 332 442 # # BplI: # [GAGNNNNNCTC] # 0 0 32 122 1097 # # BsaAI: # [YACGTR] # 328 923 # # BsaBI: # [GATNNNNATC] # 243 1008 # # BsaXI: # [ACNNNNNCTCC] # 30 30 30 71 187 274 # 629 # # BseBI: # [CCWGG] # 147 523 581 # # BseRI: # [GAGGAG] # 172 211 233 635 # # BsiI*: # [CACGAG] # 481 770 # # BsiYI*: # [CCNNNNNNNGG] # 76 435 740 # # BslFI: # [GGGAC] # 69 584 598 # # BsmAI: # [GTCTC] # 189 201 422 439 # # Bsp1407I: # [TGTACA] # 493 758 # # BspMII*: # [TCCGGA] # 186 1065 # # BsrI: # [ACTGG] # 46 55 167 207 249 527 # # BssKI: # [CCNGG] # 5 145 246 279 576 # # BstAPI: # [GCANNNNNTGC] # 513 738 # # BstEII: # [GGTNACC] # 608 643 # # BstKTI: # [GATC] # 6 15 37 50 80 234 # 239 241 349 # # Cac8I: # [GCNNGC] # 29 321 353 548 # # CauII*: # [CCSGG] # 251 277 723 # # ClaI: # [ATCGAT] # 532 719 # # Csp6I: # [GTAC] # 79 105 165 222 252 428 # # CviAII: # [CATG] # 24 164 190 873 # # CviJI: # [RGCY] # 5 8 11 21 22 24 # 27 28 29 31 36 47 # 50 64 70 86 92 118 # 144 338 # # CviRI*: # [TGCA] # 39 41 108 261 369 433 # # DdeI: # [CTNAG] # 202 1049 # # DpnI: # [GATC] # 6 16 37 50 80 234 # 239 240 349 # # DraII: # [RGGNCCY] # 47 282 922 # # DrdI: # [GACNNNNNNGTC] # 15 542 694 # # EciI: # [GGCGGA] # 84 1167 # # Eco31I: # [GGTCTC] # 439 812 # # Eco57I: # [CTGAAG] # 70 435 746 # # Eco57MI: # [CTGRAG] # 70 98 99 155 394 435 # # EcoRII: # [CCWGG] # 145 525 581 # # Esp3I: # [CGTCTC] # 201 1050 # # FalI: # [AAGNNNNNCTT] # 0 0 32 414 805 # # FatI: # [CATG] # 24 165 190 872 # # FauI: # [CCCGC] # 332 919 # # FnuDII*: # [CGCG] # 259 992 # # GsuI: # [CTGGAG] # 98 155 464 534 # # HaeIII: # [GGCC] # 27 50 116 145 332 581 # # HgiJII*: # [GRGCYC] # 435 816 # # Hin4I: # [GAYNNNNNVTC] # 0 0 4 32 32 32 # 32 32 56 61 69 274 # 627 # # Hin4II*: # [CCTTC] # 204 207 840 # # HindII: # [GTYRAC] # 12 15 537 687 # # HinfI: # [GANTC] # 6 22 88 177 278 298 # 382 # # HpaII: # [CCGG] # 187 251 278 535 # # HphI: # [GGTGA] # 21 102 120 140 164 169 # 201 334 # # Hpy166II: # [GTNNAC] # 12 15 17 36 157 276 # 327 411 # # Hpy178III*: # [TCNNGA] # 6 22 27 44 48 117 # 130 139 219 239 260 # # Hpy188I: # [TCNGA] # 37 66 78 228 416 426 # # Hpy99I: # [CGWCG] # 578 673 # # Ksp632I*: # [CTCTTC] # 18 27 136 347 723 # # MaeI: # [CTAG] # 374 434 443 # # MaeII: # [ACGT] # 23 34 121 151 227 296 # 399 # # MaeIII: # [GTNAC] # 111 497 643 # # MboI: # [GATC] # 6 18 37 50 80 234 # 238 239 349 # # MboII: # [GAAGA] # 18 27 76 166 294 330 # 340 # # McrI*: # [CGRYCG] # 321 930 # # MfeI: # [CAATTG] # 511 740 # # MlyI: # [GAGTC] # 94 208 263 298 388 # # MmeI: # [TCCRAC] # 101 216 228 276 430 # # MnlI: # [CCTC] # 25 35 41 49 131 148 # 183 185 190 264 # # MseI: # [TTAA] # 3 12 26 141 161 201 # 707 # # MwoI: # [GCNNNNNNNGC] # 311 427 513 # # NlaIII: # [CATG] # 24 161 190 876 # # NlaIV: # [GGNNCC] # 39 46 93 152 448 473 # # NruI: # [TCGCGA] # 259 992 # # NspI: # [RCATGY] # 351 900 # # PleI: # [GAGTC] # 94 207 264 298 388 # # PpuMI: # [RGGWCCY] # 47 282 922 # # PstI: # [CTGCAG] # 39 1212 # # RsaI: # [GTAC] # 79 105 165 221 252 429 # # SapI: # [GCTCTTC] # 365 886 # # ScrFI: # [CCNGG] # 5 147 246 277 576 # # SduI: # [GDGCHC] # 435 816 # # SecI*: # [CCNNGG] # 17 129 192 411 502 # # SetI: # [ASST] # 8 9 9 15 19 21 # 22 23 24 36 53 61 # 69 74 83 112 113 132 # 152 216 # # SfaNI: # [GCATC] # 315 936 # # SfeI*: # [CTRYAG] # 43 1208 # # SmlI: # [CTYRAG] # 139 1112 # # SnaBI: # [TACGTA] # 328 923 # # SphI: # [GCATGC] # 351 900 # # SplI*: # [CGTACG] # 328 923 # # StyI: # [CCWWGG] # 17 192 411 631 # # TaiI: # [ACGT] # 23 31 121 151 227 299 # 399 # # TaqI: # [TCGA] # 139 247 285 580 # # TatI: # [WGTACW] # 493 758 # # TauI: # [GCSGC] # 67 1184 # # TfiI: # [GAWTC] # 22 543 686 # # TseI: # [GCWGC] # 76 268 400 507 # # Tsp45I: # [GTSAC] # 111 497 643 # # Tsp4CI*: # [ACNGT] # 19 44 99 111 129 209 # 640 # # TspEI: # [AATT] # 34 49 80 123 147 158 # 229 431 # # TspGWI: # [ACGGA] # 62 74 205 446 464 # # TspRI: # [CASTGNN] # 108 187 956 # # XbaI: # [TCTAGA] # 435 816 # # XhoII: # [RGATCY] # 238 289 724 # # ZraI: # [GACGTC] # 207 1044 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1251 # Reported_sequences: 1 # Reported_hitcount: 371 #---------------------------------------