Restriction Fragments for YHRWtau3



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

YHRWtau3 on chromosome VIII from coordinates 146844 to 147114.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.27631.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 30 34 + Tsp4CI* ACNGT 32 31 . . 44 48 - TspDTI ATGAA 34 32 . . 63 66 + CviRI* TGCA 64 64 . . 64 75 + AlfI GCANNNNNNTGC 53 51 87 85 65 74 + MslI CAYNNNNRTG 69 69 . . 65 68 + FatI CATG 64 68 . . 65 68 + NlaIII CATG 68 64 . . 65 68 + CviAII CATG 65 67 . . 67 72 - TaqII CACCCA 57 55 . . 64 75 - AlfI GCANNNNNNTGC 87 85 53 51 80 83 + CviRI* TGCA 81 81 . . 84 88 + MboII GAAGA 96 95 . . 84 89 + BbvII* GAAGAC 91 95 . . 111 115 - BsmAI GTCTC 105 109 . . 129 132 + SetI ASST 132 128 . . 128 131 - MnlI CCTC 121 120 . . 134 140 + DraII RGGNCCY 135 138 . . 135 138 + CviJI RGCY 136 136 . . 135 138 + HaeIII GGCC 136 136 . . 135 139 + BmgT120I GGNCC 136 137 . . 135 139 + AsuI* GGNCC 135 138 . . 151 160 + MslI CAYNNNNRTG 155 155 . . 159 162 + CviRI* TGCA 160 160 . . 166 175 + MslI CAYNNNNRTG 170 170 . . 156 166 - Hin4I GAYNNNNNVTC 179 174 147 142 183 188 - TaqII CACCCA 173 171 . . 188 192 + Tsp45I GTSAC 187 192 . . 188 192 + MaeIII GTNAC 187 192 . . 194 197 + MseI TTAA 194 196 . . 203 208 + Hpy178III* TCNNGA 204 206 . . 205 209 + Tsp45I GTSAC 204 209 . . 205 209 + MaeIII GTNAC 204 209 . . 217 221 - MboII GAAGA 209 208 . . 239 242 + MseI TTAA 239 241 . . 242 245 + SetI ASST 245 241 . . 243 247 + HphI GGTGA 255 254 . . 244 249 + Hpy166II GTNNAC 246 246 . . 254 257 + AciI CCGC 254 256 . . 256 261 + NspI RCATGY 260 256 . . 257 260 + FatI CATG 256 260 . . 257 260 + NlaIII CATG 260 256 . . 257 260 + CviAII CATG 257 259 . . #--------------------------------------- # # # AciI: # [CCGC] # 17 254 # # AlfI: # [GCANNNNNNTGC] # 0 0 34 53 184 # # AsuI*: # [GGNCC] # 135 136 # # BbvII*: # [GAAGAC] # 91 180 # # BmgT120I: # [GGNCC] # 135 136 # # BsmAI: # [GTCTC] # 105 166 # # CviAII: # [CATG] # 14 65 192 # # CviJI: # [RGCY] # 135 136 # # CviRI*: # [TGCA] # 17 64 79 111 # # DraII: # [RGGNCCY] # 135 136 # # FatI: # [CATG] # 15 64 192 # # HaeIII: # [GGCC] # 135 136 # # Hin4I: # [GAYNNNNNVTC] # 32 92 147 # # HphI: # [GGTGA] # 16 255 # # Hpy166II: # [GTNNAC] # 25 246 # # Hpy178III*: # [TCNNGA] # 67 204 # # MaeIII: # [GTNAC] # 17 67 187 # # MboII: # [GAAGA] # 62 96 113 # # MnlI: # [CCTC] # 121 150 # # MseI: # [TTAA] # 32 45 194 # # MslI: # [CAYNNNNRTG] # 15 69 86 101 # # NlaIII: # [CATG] # 11 68 192 # # NspI: # [RCATGY] # 11 260 # # SetI: # [ASST] # 26 113 132 # # TaqII: # [CACCCA] # 57 98 116 # # Tsp45I: # [GTSAC] # 17 67 187 # # Tsp4CI*: # [ACNGT] # 32 239 # # TspDTI: # [ATGAA] # 34 237 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 271 # Reported_sequences: 1 # Reported_hitcount: 42 #---------------------------------------