Restriction Fragments for YHRCsigma3



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The currently selected gene/sequence is :

YHRCsigma3 on chromosome VIII from coordinates 466975 to 466884.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.14805.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 22 25 + TaiI ACGT 25 21 . . 22 25 + SetI ASST 25 21 . . 22 25 + MaeII ACGT 22 24 . . 19 29 - BaeI ACNNNNGTAYC 44 39 11 6 32 42 + Hin4I GAYNNNNNVTC 23 18 55 50 32 42 + Eam1105I GACNNNNNGTC 37 36 . . 38 41 + TaiI ACGT 41 37 . . 38 41 + SetI ASST 41 37 . . 38 41 + MaeII ACGT 38 40 . . 32 42 - Hin4I GAYNNNNNVTC 55 50 23 18 42 46 - FokI GGATG 28 32 . . 42 46 - BseGI GGATG 41 39 . . 49 54 + AclI AACGTT 50 52 . . 50 53 + TaiI ACGT 53 49 . . 50 53 + SetI ASST 53 49 . . 50 53 + MaeII ACGT 50 52 . . 53 57 - TspDTI ATGAA 43 41 . . 87 91 + MaeIII GTNAC 86 91 . . #--------------------------------------- # # # AclI: # [AACGTT] # 42 50 # # BaeI: # [ACNNNNGTAYC] # 11 33 48 # # BseGI: # [GGATG] # 41 51 # # Eam1105I: # [GACNNNNNGTC] # 37 55 # # FokI: # [GGATG] # 28 64 # # Hin4I: # [GAYNNNNNVTC] # 0 0 23 32 37 # # MaeII: # [ACGT] # 12 16 22 42 # # MaeIII: # [GTNAC] # 6 86 # # SetI: # [ASST] # 12 16 25 39 # # TaiI: # [ACGT] # 12 16 25 39 # # TspDTI: # [ATGAA] # 43 49 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 92 # Reported_sequences: 1 # Reported_hitcount: 18 #---------------------------------------