Restriction Fragments for YHRCdelta6



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The currently selected gene/sequence is :

YHRCdelta6 on chromosome VIII from coordinates 133684 to 133565.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.24781.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 12 16 + TfiI GAWTC 12 15 . . 9 12 - MnlI CCTC 2 1 . . 12 16 + HinfI GANTC 12 15 . . 37 42 + SpeI ACTAGT 37 41 . . 38 41 + MaeI CTAG 38 40 . . 49 52 + CviRI* TGCA 50 50 . . 54 57 + SetI ASST 57 53 . . 54 57 + CviJI RGCY 55 55 . . 54 57 + AluI AGCT 55 55 . . 58 63 + EcoRV GATATC 60 60 . . 66 69 + CviJI RGCY 67 67 . . 68 72 + Hin4II* CCTTC 78 77 . . 71 75 - HphI GGTGA 63 62 . . 98 101 + MseI TTAA 98 100 . . 113 118 + SspI AATATT 115 115 . . #--------------------------------------- # # # AluI: # [AGCT] # 55 65 # # CviJI: # [RGCY] # 12 53 55 # # CviRI*: # [TGCA] # 50 70 # # EcoRV: # [GATATC] # 60 60 # # Hin4II*: # [CCTTC] # 42 78 # # HinfI: # [GANTC] # 12 108 # # HphI: # [GGTGA] # 57 63 # # MaeI: # [CTAG] # 38 82 # # MnlI: # [CCTC] # 2 118 # # MseI: # [TTAA] # 22 98 # # SetI: # [ASST] # 57 63 # # SpeI: # [ACTAGT] # 37 83 # # SspI: # [AATATT] # 5 115 # # TfiI: # [GAWTC] # 12 108 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 120 # Reported_sequences: 1 # Reported_hitcount: 15 #---------------------------------------