Restriction Fragments for YHRCdelta12



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The currently selected gene/sequence is :

YHRCdelta12 on chromosome VIII from coordinates 464147 to 463920.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.10302.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 29 33 + Tsp45I GTSAC 28 33 . . 29 33 + MaeIII GTNAC 28 33 . . 31 41 + Hin4I GAYNNNNNVTC 22 17 54 49 31 41 + Eam1105I GACNNNNNGTC 36 35 . . 32 37 + SpeI ACTAGT 32 36 . . 33 36 + MaeI CTAG 33 35 . . 31 41 - Hin4I GAYNNNNNVTC 54 49 22 17 41 44 + FatI CATG 40 44 . . 41 44 + NlaIII CATG 44 40 . . 41 44 + CviAII CATG 41 43 . . 44 48 + MaeIII GTNAC 43 48 . . 62 70 + DraIII CACNNNGTG 67 64 . . 64 67 + FnuDII* CGCG 65 65 . . 65 68 - AciI CCGC 65 67 . . 74 78 + MboII GAAGA 86 85 . . 91 95 + BslFI GGGAC 105 109 . . 93 101 + Tth111I GACNNNGTC 96 97 . . 93 104 + DrdI GACNNNNNNGTC 99 97 . . 96 100 + Tsp4CI* ACNGT 98 97 . . 101 105 + Hpy99I CGWCG 105 100 . . 103 106 + TaqI TCGA 103 105 . . 107 110 + TspEI AATT 106 110 . . 108 113 + VspI ATTAAT 109 111 . . 109 112 + MseI TTAA 109 111 . . 117 120 + SetI ASST 120 116 . . 117 120 + CviJI RGCY 118 118 . . 117 120 + AluI AGCT 118 118 . . 125 128 + CviRI* TGCA 126 126 . . 147 151 + FokI GGATG 160 164 . . 147 151 + BseGI GGATG 153 151 . . 152 156 + TspDTI ATGAA 167 165 . . 179 182 + SetI ASST 182 178 . . 177 181 - Hin4II* CCTTC 171 170 . . 184 187 + TspEI AATT 183 187 . . 185 190 + VspI ATTAAT 186 188 . . 186 189 + MseI TTAA 186 188 . . 188 193 + SspI AATATT 190 190 . . 193 198 + Hpy178III* TCNNGA 194 196 . . 193 198 + BspHI TCATGA 193 197 . . 194 197 + FatI CATG 193 197 . . 194 197 + NlaIII CATG 197 193 . . 194 197 + CviAII CATG 194 196 . . 192 196 - TspDTI ATGAA 182 180 . . 207 212 + TaqII GACCGA 223 221 . . 211 215 + TfiI GAWTC 211 214 . . 211 215 + HinfI GANTC 211 214 . . 215 225 + BsiYI* CCNNNNNNNGG 221 218 . . 215 225 + PflMI CCANNNNNTGG 221 218 . . #--------------------------------------- # # # AciI: # [CCGC] # 65 163 # # AluI: # [AGCT] # 110 118 # # BseGI: # [GGATG] # 75 153 # # BsiYI*: # [CCNNNNNNNGG] # 7 221 # # BslFI: # [GGGAC] # 105 123 # # BspHI: # [TCATGA] # 35 193 # # CviAII: # [CATG] # 34 41 153 # # CviJI: # [RGCY] # 110 118 # # CviRI*: # [TGCA] # 102 126 # # DraIII: # [CACNNNGTG] # 67 161 # # DrdI: # [GACNNNNNNGTC] # 99 129 # # Eam1105I: # [GACNNNNNGTC] # 36 192 # # FatI: # [CATG] # 35 40 153 # # FnuDII*: # [CGCG] # 65 163 # # FokI: # [GGATG] # 68 160 # # Hin4I: # [GAYNNNNNVTC] # 0 0 22 32 174 # # Hin4II*: # [CCTTC] # 57 171 # # HinfI: # [GANTC] # 17 211 # # Hpy178III*: # [TCNNGA] # 34 194 # # Hpy99I: # [CGWCG] # 105 123 # # MaeI: # [CTAG] # 33 195 # # MaeIII: # [GTNAC] # 15 28 185 # # MboII: # [GAAGA] # 86 142 # # MseI: # [TTAA] # 42 77 109 # # NlaIII: # [CATG] # 31 44 153 # # PflMI: # [CCANNNNNTGG] # 7 221 # # SetI: # [ASST] # 46 62 120 # # SpeI: # [ACTAGT] # 32 196 # # SspI: # [AATATT] # 38 190 # # TaqI: # [TCGA] # 103 125 # # TaqII: # [GACCGA] # 5 223 # # TfiI: # [GAWTC] # 17 211 # # Tsp45I: # [GTSAC] # 28 200 # # Tsp4CI*: # [ACNGT] # 98 130 # # TspDTI: # [ATGAA] # 15 46 167 # # TspEI: # [AATT] # 45 77 106 # # Tth111I: # [GACNNNGTC] # 96 132 # # VspI: # [ATTAAT] # 42 77 109 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 228 # Reported_sequences: 1 # Reported_hitcount: 48 #---------------------------------------