Restriction Fragments for YHR219C-A



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The currently selected gene/sequence is :

YHR219C-A on chromosome VIII from coordinates 561681 to 561199.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.22436.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 6 11 + Cac8I GCNNGC 8 8 . . 16 21 + PstI CTGCAG 20 16 . . 16 21 + SfeI* CTRYAG 16 20 . . 17 20 + CviRI* TGCA 18 18 . . 18 22 + BlsI GCNGC 20 19 . . 18 22 + BisI GCNGC 19 20 . . 18 22 + BbvI GCAGC 30 34 . . 18 22 + TseI GCWGC 18 21 . . 20 23 + SetI ASST 23 19 . . 20 23 + CviJI RGCY 21 21 . . 20 23 + AluI AGCT 21 21 . . 26 29 + TaiI ACGT 29 25 . . 26 29 + SetI ASST 29 25 . . 26 29 + MaeII ACGT 26 28 . . 28 31 + Csp6I GTAC 28 30 . . 28 31 + RsaI GTAC 29 29 . . 33 43 + MwoI GCNNNNNNNGC 39 36 . . 35 40 + NlaIV GGNNCC 37 37 . . 35 39 + BmgT120I GGNCC 36 37 . . 35 39 + AvaII GGWCC 35 38 . . 35 39 + AsuI* GGNCC 35 38 . . 33 36 - AciI CCGC 33 35 . . 36 47 + DrdI GACNNNNNNGTC 42 40 . . 40 45 + PstI CTGCAG 44 40 . . 40 45 + SfeI* CTRYAG 40 44 . . 41 44 + CviRI* TGCA 42 42 . . 49 54 + HaeII RGCGCY 53 49 . . 49 54 + Eco47III AGCGCT 51 51 . . 50 53 + Hin6I GCGC 50 52 . . 50 53 + GlaI GCGC 51 51 . . 50 53 + HhaI GCGC 52 50 . . 58 61 + FatI CATG 57 61 . . 58 61 + NlaIII CATG 61 57 . . 58 61 + CviAII CATG 58 60 . . 82 87 + MmeI TCCRAC 107 105 . . 90 95 + Hpy178III* TCNNGA 91 93 . . 91 96 + SmlI CTYRAG 91 95 . . 91 96 + XhoI CTCGAG 91 95 . . 91 96 + BmeT110I CYCGRG 92 94 . . 91 96 + AvaI CYCGRG 91 95 . . 92 95 + TaqI TCGA 92 94 . . 95 100 + HaeII RGCGCY 99 95 . . 95 100 + Eco47III AGCGCT 97 97 . . 96 99 + Hin6I GCGC 96 98 . . 96 99 + GlaI GCGC 97 97 . . 96 99 + HhaI GCGC 98 96 . . 109 120 + DrdI GACNNNNNNGTC 115 113 . . 110 114 + Tsp4CI* ACNGT 112 111 . . 126 129 + MnlI CCTC 136 135 . . 134 138 - MboII GAAGA 126 125 . . 138 142 + BssKI CCNGG 137 142 . . 138 142 + ScrFI CCNGG 139 140 . . 138 142 + CauII* CCSGG 139 140 . . 139 142 + HpaII CCGG 139 141 . . 154 158 - BslFI GGGAC 139 143 . . 157 161 - BsrI ACTGG 157 155 . . 156 161 - BfiI ACTGGG 151 150 . . 168 173 + TatI WGTACW 168 172 . . 168 173 + ScaI AGTACT 170 170 . . 169 172 + Csp6I GTAC 169 171 . . 169 172 + RsaI GTAC 170 170 . . 172 177 + PstI CTGCAG 176 172 . . 172 177 + SfeI* CTRYAG 172 176 . . 173 176 + CviRI* TGCA 174 174 . . 174 178 + BlsI GCNGC 176 175 . . 174 178 + BisI GCNGC 175 176 . . 174 178 + BbvI GCAGC 186 190 . . 174 178 + TseI GCWGC 174 177 . . 179 182 + SetI ASST 182 178 . . 180 183 + MnlI CCTC 190 189 . . 189 192 + TaqI TCGA 189 191 . . 186 190 - MboII GAAGA 178 177 . . 185 190 - BbvII* GAAGAC 178 182 . . 191 195 + TfiI GAWTC 191 194 . . 191 195 + HinfI GANTC 191 194 . . 215 220 + PsiI TTATAA 217 217 . . 232 235 + DpnI GATC 233 233 . . 232 235 + BstKTI GATC 234 232 . . 232 235 + MboI GATC 231 235 . . 238 242 + Hin4II* CCTTC 248 247 . . 241 246 + Hpy178III* TCNNGA 242 244 . . 246 251 + NspI RCATGY 250 246 . . 247 250 + FatI CATG 246 250 . . 247 250 + NlaIII CATG 250 246 . . 247 250 + CviAII CATG 247 249 . . 250 253 - AciI CCGC 250 252 . . 261 264 + CviJI RGCY 262 262 . . 261 266 + NlaIV GGNNCC 263 263 . . 270 275 + Cac8I GCNNGC 272 272 . . 275 278 + MnlI CCTC 285 284 . . 282 285 + TaqI TCGA 282 284 . . 284 288 + TfiI GAWTC 284 287 . . 284 288 + HinfI GANTC 284 287 . . 298 301 + TspEI AATT 297 301 . . 306 311 + Ksp632I* CTCTTC 312 315 . . 307 311 - MboII GAAGA 299 298 . . 310 314 - MboII GAAGA 302 301 . . 314 317 + MaeI CTAG 314 316 . . 321 326 + BalI TGGCCA 323 323 . . 321 326 + CfrI YGGCCR 321 325 . . 322 325 + CviJI RGCY 323 323 . . 322 325 + HaeIII GGCC 323 323 . . 324 328 + BssKI CCNGG 323 328 . . 324 328 + BseBI CCWGG 325 326 . . 324 328 + ScrFI CCNGG 325 326 . . 324 328 + EcoRII CCWGG 323 328 . . 326 329 + SetI ASST 329 325 . . 327 332 + KpnI GGTACC 331 327 . . 327 332 + NlaIV GGNNCC 329 329 . . 327 332 + HgiCI* GGYRCC 327 331 . . 327 332 + Acc65I GGTACC 327 331 . . 328 331 + Csp6I GTAC 328 330 . . 328 331 + RsaI GTAC 329 329 . . 339 342 + TspEI AATT 338 342 . . 331 344 - AjuI GAANNNNNNNTTGG 356 351 324 319 351 355 + Hpy188I TCNGA 353 352 . . 352 356 + DdeI CTNAG 352 355 . . 352 356 - BspCNI CTCAG 344 342 . . 352 356 - BseMII CTCAG 343 341 . . 356 366 + BplI GAGNNNNNCTC 347 342 379 374 360 365 + XhoII RGATCY 360 364 . . 360 365 + BglII AGATCT 360 364 . . 361 364 + DpnI GATC 362 362 . . 361 364 + BstKTI GATC 363 361 . . 361 364 + MboI GATC 360 364 . . 354 364 - Hin4I GAYNNNNNVTC 377 372 345 340 356 366 - BplI GAGNNNNNCTC 379 374 347 342 385 390 + ApoI RAATTY 385 389 . . 386 389 + TspEI AATT 385 389 . . 399 404 + SfeI* CTRYAG 399 403 . . 404 410 + SanDI GGGWCCC 405 408 . . 404 410 + PpuMI RGGWCCY 405 408 . . 404 410 + DraII RGGNCCY 405 408 . . 404 409 + NlaIV GGNNCC 406 406 . . 405 410 + NlaIV GGNNCC 407 407 . . 405 409 + BmgT120I GGNCC 406 407 . . 405 409 + AvaII GGWCC 405 408 . . 405 409 + AsuI* GGNCC 405 408 . . 406 410 - BslFI GGGAC 391 395 . . 410 420 + BsiYI* CCNNNNNNNGG 416 413 . . 410 420 + PflMI CCANNNNNTGG 416 413 . . 411 414 + FatI CATG 410 414 . . 411 414 + NlaIII CATG 414 410 . . 411 414 + CviAII CATG 411 413 . . 419 422 + CviJI RGCY 420 420 . . 422 425 + TaqI TCGA 422 424 . . 428 432 - MboII GAAGA 420 419 . . 427 432 - BbvII* GAAGAC 420 424 . . 450 453 + MnlI CCTC 460 459 . . 462 466 + BlsI GCNGC 464 463 . . 462 466 + BisI GCNGC 463 464 . . 462 466 + BbvI GCAGC 474 478 . . 462 466 + TseI GCWGC 462 465 . . 465 470 - BsmI GAATGC 465 463 . . 478 481 + SetI ASST 481 477 . . 478 481 + CviJI RGCY 479 479 . . 478 481 + AluI AGCT 479 479 . . 480 483 + MaeI CTAG 480 482 . . #--------------------------------------- # # # Acc65I: # [GGTACC] # 156 327 # # AciI: # [CCGC] # 33 217 233 # # AjuI: # [GAANNNNNNNTTGG] # 32 127 324 # # AluI: # [AGCT] # 4 21 458 # # ApoI: # [RAATTY] # 98 385 # # AsuI*: # [GGNCC] # 35 78 370 # # AvaI: # [CYCGRG] # 91 392 # # AvaII: # [GGWCC] # 35 78 370 # # BalI: # [TGGCCA] # 160 323 # # BbvI: # [GCAGC] # 9 30 156 288 # # BbvII*: # [GAAGAC] # 63 178 242 # # BfiI: # [ACTGGG] # 151 332 # # BglII: # [AGATCT] # 123 360 # # BisI: # [GCNGC] # 19 20 156 288 # # BlsI: # [GCNGC] # 19 20 156 288 # # BmeT110I: # [CYCGRG] # 92 391 # # BmgT120I: # [GGNCC] # 36 77 370 # # BplI: # [GAGNNNNNCTC] # 0 0 32 104 347 # # BseBI: # [CCWGG] # 158 325 # # BseMII: # [CTCAG] # 140 343 # # BsiYI*: # [CCNNNNNNNGG] # 67 416 # # BslFI: # [GGGAC] # 92 139 252 # # BsmI: # [GAATGC] # 18 465 # # BspCNI: # [CTCAG] # 139 344 # # BsrI: # [ACTGG] # 157 326 # # BssKI: # [CCNGG] # 137 160 186 # # BstKTI: # [GATC] # 120 129 234 # # Cac8I: # [GCNNGC] # 8 211 264 # # CauII*: # [CCSGG] # 139 344 # # CfrI: # [YGGCCR] # 162 321 # # Csp6I: # [GTAC] # 28 141 155 159 # # CviAII: # [CATG] # 58 72 164 189 # # CviJI: # [RGCY] # 4 21 59 61 97 241 # # CviRI*: # [TGCA] # 18 24 132 309 # # DdeI: # [CTNAG] # 131 352 # # DpnI: # [GATC] # 121 129 233 # # DraII: # [RGGNCCY] # 78 405 # # DrdI: # [GACNNNNNNGTC] # 42 73 368 # # Eco47III: # [AGCGCT] # 46 51 386 # # EcoRII: # [CCWGG] # 160 323 # # FatI: # [CATG] # 57 73 164 189 # # GlaI: # [GCGC] # 46 51 386 # # HaeII: # [RGCGCY] # 46 53 384 # # HaeIII: # [GGCC] # 160 323 # # HgiCI*: # [GGYRCC] # 156 327 # # HhaI: # [GCGC] # 46 52 385 # # Hin4I: # [GAYNNNNNVTC] # 32 106 345 # # Hin4II*: # [CCTTC] # 235 248 # # Hin6I: # [GCGC] # 46 50 387 # # HinfI: # [GANTC] # 93 191 199 # # HpaII: # [CCGG] # 139 344 # # Hpy178III*: # [TCNNGA] # 91 151 241 # # Hpy188I: # [TCNGA] # 130 353 # # KpnI: # [GGTACC] # 152 331 # # Ksp632I*: # [CTCTTC] # 171 312 # # MaeI: # [CTAG] # 3 166 314 # # MaeII: # [ACGT] # 26 457 # # MboI: # [GATC] # 123 129 231 # # MboII: # [GAAGA] # 3 52 63 118 121 126 # # MmeI: # [TCCRAC] # 107 376 # # MnlI: # [CCTC] # 23 54 95 136 175 # # MwoI: # [GCNNNNNNNGC] # 39 444 # # NlaIII: # [CATG] # 61 69 164 189 # # NlaIV: # [GGNNCC] # 1 37 66 76 77 226 # # NspI: # [RCATGY] # 233 250 # # PflMI: # [CCANNNNNTGG] # 67 416 # # PpuMI: # [RGGWCCY] # 78 405 # # PsiI: # [TTATAA] # 217 266 # # PstI: # [CTGCAG] # 20 24 132 307 # # RsaI: # [GTAC] # 29 141 154 159 # # SanDI: # [GGGWCCC] # 78 405 # # ScaI: # [AGTACT] # 170 313 # # ScrFI: # [CCNGG] # 139 158 186 # # SetI: # [ASST] # 2 6 23 147 152 153 # # SfeI*: # [CTRYAG] # 16 24 84 132 227 # # SmlI: # [CTYRAG] # 91 392 # # TaiI: # [ACGT] # 29 454 # # TaqI: # [TCGA] # 61 92 93 97 140 # # TatI: # [WGTACW] # 168 315 # # TfiI: # [GAWTC] # 93 191 199 # # TseI: # [GCWGC] # 18 21 156 288 # # Tsp4CI*: # [ACNGT] # 112 371 # # TspEI: # [AATT] # 41 47 98 297 # # XhoI: # [CTCGAG] # 91 392 # # XhoII: # [RGATCY] # 123 360 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 483 # Reported_sequences: 1 # Reported_hitcount: 158 #---------------------------------------