Restriction Fragments for YHR214C-D



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The currently selected gene/sequence is :

YHR214C-D on chromosome VIII from coordinates 550941 to 550648.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.3505.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 6 10 + BmgT120I GGNCC 7 8 . . 6 10 + AvaII GGWCC 6 9 . . 6 10 + AsuI* GGNCC 6 9 . . 12 21 + MslI CAYNNNNRTG 16 16 . . 12 21 + OliI CACNNNNGTG 16 16 . . 20 25 + BalI TGGCCA 22 22 . . 20 25 + CfrI YGGCCR 20 24 . . 21 24 + CviJI RGCY 22 22 . . 21 24 + HaeIII GGCC 22 22 . . 23 27 + BccI CCATC 31 32 . . 46 50 + HphI GGTGA 58 57 . . 47 52 + Hpy166II GTNNAC 49 49 . . 51 54 + SetI ASST 54 50 . . 58 61 + FatI CATG 57 61 . . 58 61 + NlaIII CATG 61 57 . . 58 61 + CviAII CATG 58 60 . . 71 76 + SfeI* CTRYAG 71 75 . . 90 94 + BlsI GCNGC 92 91 . . 90 94 + BisI GCNGC 91 92 . . 90 94 + TseI GCWGC 90 93 . . 90 94 - BbvI GCAGC 77 81 . . 99 103 - TspDTI ATGAA 89 87 . . 101 105 - FokI GGATG 87 91 . . 101 105 - BseGI GGATG 100 98 . . 107 110 + MaeI CTAG 107 109 . . 110 115 + EcoRI GAATTC 110 114 . . 110 115 + ApoI RAATTY 110 114 . . 111 114 + TspEI AATT 110 114 . . 114 119 + Hpy178III* TCNNGA 115 117 . . 114 119 + BspHI TCATGA 114 118 . . 115 118 + FatI CATG 114 118 . . 115 118 + NlaIII CATG 118 114 . . 115 118 + CviAII CATG 115 117 . . 113 117 - TspDTI ATGAA 103 101 . . 119 122 + SetI ASST 122 118 . . 118 121 - MnlI CCTC 111 110 . . 138 143 + PsiI TTATAA 140 140 . . 157 160 + SetI ASST 160 156 . . 157 163 + SexAI ACCWGGT 157 162 . . 158 162 + BssKI CCNGG 157 162 . . 158 162 + BseBI CCWGG 159 160 . . 158 162 + ScrFI CCNGG 159 160 . . 158 162 + EcoRII CCWGG 157 162 . . 158 168 + BsiYI* CCNNNNNNNGG 164 161 . . 183 188 + ApoI RAATTY 183 187 . . 184 187 + TspEI AATT 183 187 . . 194 197 + MseI TTAA 194 196 . . 198 203 + BetI* WCCGGW 198 202 . . 199 202 + HpaII CCGG 199 201 . . 210 214 + Hpy188I TCNGA 212 211 . . 211 215 + DdeI CTNAG 211 214 . . 214 217 + SetI ASST 217 213 . . 214 217 + CviJI RGCY 215 215 . . 214 217 + AluI AGCT 215 215 . . 211 215 - BspCNI CTCAG 203 201 . . 211 215 - BseMII CTCAG 202 200 . . 222 227 - MmeI TCCRAC 203 201 . . 231 234 + TspEI AATT 230 234 . . 249 254 + EcoT22I ATGCAT 253 249 . . 250 253 + CviRI* TGCA 251 251 . . 251 255 + SfaNI GCATC 260 264 . . 257 261 + Tsp4CI* ACNGT 259 258 . . 262 265 + CviRI* TGCA 263 263 . . 264 269 - BsrDI GCAATG 263 261 . . 274 278 + Tsp4CI* ACNGT 276 275 . . 289 294 + PsiI TTATAA 291 291 . . #--------------------------------------- # # # AluI: # [AGCT] # 79 215 # # ApoI: # [RAATTY] # 73 110 111 # # AsuI*: # [GGNCC] # 6 288 # # AvaII: # [GGWCC] # 6 288 # # BalI: # [TGGCCA] # 22 272 # # BbvI: # [GCAGC] # 77 217 # # BccI: # [CCATC] # 31 263 # # BetI*: # [WCCGGW] # 96 198 # # BisI: # [GCNGC] # 91 203 # # BlsI: # [GCNGC] # 92 202 # # BmgT120I: # [GGNCC] # 7 287 # # BseBI: # [CCWGG] # 135 159 # # BseGI: # [GGATG] # 100 194 # # BseMII: # [CTCAG] # 92 202 # # BsiYI*: # [CCNNNNNNNGG] # 130 164 # # BspCNI: # [CTCAG] # 91 203 # # BspHI: # [TCATGA] # 114 180 # # BsrDI: # [GCAATG] # 31 263 # # BssKI: # [CCNGG] # 137 157 # # CfrI: # [YGGCCR] # 20 274 # # CviAII: # [CATG] # 57 58 179 # # CviJI: # [RGCY] # 22 79 193 # # CviRI*: # [TGCA] # 12 31 251 # # DdeI: # [CTNAG] # 83 211 # # EcoRI: # [GAATTC] # 110 184 # # EcoRII: # [CCWGG] # 137 157 # # EcoT22I: # [ATGCAT] # 41 253 # # FatI: # [CATG] # 57 57 180 # # FokI: # [GGATG] # 87 207 # # HaeIII: # [GGCC] # 22 272 # # HpaII: # [CCGG] # 95 199 # # HphI: # [GGTGA] # 58 236 # # Hpy166II: # [GTNNAC] # 49 245 # # Hpy178III*: # [TCNNGA] # 115 179 # # Hpy188I: # [TCNGA] # 82 212 # # MaeI: # [CTAG] # 107 187 # # MmeI: # [TCCRAC] # 91 203 # # MnlI: # [CCTC] # 111 183 # # MseI: # [TTAA] # 100 194 # # MslI: # [CAYNNNNRTG] # 16 278 # # NlaIII: # [CATG] # 57 61 176 # # OliI: # [CACNNNNGTG] # 16 278 # # PsiI: # [TTATAA] # 3 140 151 # # ScrFI: # [CCNGG] # 135 159 # # SetI: # [ASST] # 38 54 57 68 77 # # SexAI: # [ACCWGGT] # 137 157 # # SfaNI: # [GCATC] # 34 260 # # SfeI*: # [CTRYAG] # 71 223 # # TseI: # [GCWGC] # 90 204 # # Tsp4CI*: # [ACNGT] # 17 18 259 # # TspDTI: # [ATGAA] # 14 89 191 # # TspEI: # [AATT] # 47 64 73 110 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 294 # Reported_sequences: 1 # Reported_hitcount: 66 #---------------------------------------