Restriction Fragments for FLO5/YHR211W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

FLO5/YHR211W on chromosome VIII from coordinates 525392 to 528619.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.31823.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 5 10 + MfeI CAATTG 5 9 . . 6 9 + TspEI AATT 5 9 . . 9 12 + CviRI* TGCA 10 10 . . 16 22 + TspRI CASTGNN 22 13 . . 19 22 + CviRI* TGCA 20 20 . . 16 21 - BtsI GCAGTG 15 13 . . 10 21 - AlfI GCANNNNNNTGC 33 31 . . 39 42 + CviJI RGCY 40 40 . . 39 42 + HaeIII GGCC 40 40 . . 40 50 + MwoI GCNNNNNNNGC 46 43 . . 40 50 + BglI GCCNNNNNGGC 46 43 . . 54 57 + TspEI AATT 53 57 . . 55 60 + VspI ATTAAT 56 58 . . 56 59 + MseI TTAA 56 58 . . 63 66 + CviJI RGCY 64 64 . . 63 66 + HaeIII GGCC 64 64 . . 64 74 + MwoI GCNNNNNNNGC 70 67 . . 65 68 + MnlI CCTC 75 74 . . 65 71 + SauI* CCTNAGG 66 69 . . 66 70 + DdeI CTNAG 66 69 . . 66 70 + BspCNI CTCAG 79 77 . . 66 70 + BseMII CTCAG 80 78 . . 67 72 + Hpy178III* TCNNGA 68 70 . . 70 75 + NlaIV GGNNCC 72 72 . . 72 75 + CviJI RGCY 73 73 . . 73 83 + MwoI GCNNNNNNNGC 79 76 . . 73 83 + BglI GCCNNNNNGGC 79 76 . . 79 82 - MnlI CCTC 72 71 . . 82 87 + Cac8I GCNNGC 84 84 . . 92 97 + EcoP15I CAGCAG 122 124 . . 94 99 + Cac8I GCNNGC 96 96 . . 97 100 + CviJI RGCY 98 98 . . 97 100 + HaeIII GGCC 98 98 . . 99 113 + XcmI CCANNNNNNNNNTGG 106 105 . . 102 105 - MnlI CCTC 95 94 . . 113 117 + FokI GGATG 126 130 . . 113 117 + BseGI GGATG 119 117 . . 115 119 + TspDTI ATGAA 130 128 . . 123 128 + ApoI RAATTY 123 127 . . 124 127 + TspEI AATT 123 127 . . 132 136 - BsrI ACTGG 132 130 . . 138 142 - TspDTI ATGAA 128 126 . . 148 152 + TfiI GAWTC 148 151 . . 148 152 + HinfI GANTC 148 151 . . 152 155 + MnlI CCTC 162 161 . . 162 167 + AsuII TTCGAA 163 165 . . 163 166 + TaqI TCGA 163 165 . . 165 170 + BsmI GAATGC 171 169 . . 168 171 + CviRI* TGCA 169 169 . . 169 173 + BlsI GCNGC 171 170 . . 169 173 + BisI GCNGC 170 171 . . 169 173 + BbvI GCAGC 181 185 . . 169 173 + TseI GCWGC 169 172 . . 172 182 + MwoI GCNNNNNNNGC 178 175 . . 180 183 + CviJI RGCY 181 181 . . 194 197 + MnlI CCTC 204 203 . . 208 211 + MaeI CTAG 208 210 . . 210 213 + SetI ASST 213 209 . . 218 222 + Hpy188I TCNGA 220 219 . . 217 222 - MmeI TCCRAC 198 196 . . 221 224 - MnlI CCTC 214 213 . . 238 241 + TaqI TCGA 238 240 . . 246 251 + PsiI TTATAA 248 248 . . 250 255 + SspI AATATT 252 252 . . 267 271 - TspDTI ATGAA 257 255 . . 289 292 + MnlI CCTC 299 298 . . 290 295 + SmlI CTYRAG 290 294 . . 291 296 + Hpy178III* TCNNGA 292 294 . . 295 299 + MboII GAAGA 307 306 . . 290 295 - Bce83I* CTTGAG 275 273 . . 298 302 + TfiI GAWTC 298 301 . . 298 302 + HinfI GANTC 298 301 . . 311 315 + BsrI ACTGG 316 314 . . 311 316 + BfiI ACTGGG 321 320 . . 316 320 + FokI GGATG 329 333 . . 316 320 + BseGI GGATG 322 320 . . 319 322 + CviRI* TGCA 320 320 . . 317 321 - SfaNI GCATC 307 311 . . 329 334 - TaqII CACCCA 319 317 . . 354 357 + TspEI AATT 353 357 . . 357 360 + CviRI* TGCA 358 358 . . 362 366 + BsrI ACTGG 367 365 . . 363 368 + Eco57MI CTGRAG 384 382 . . 363 368 + GsuI CTGGAG 384 382 . . 367 372 + TatI WGTACW 367 371 . . 367 372 + ScaI AGTACT 369 369 . . 368 371 + Csp6I GTAC 368 370 . . 368 371 + RsaI GTAC 369 369 . . 404 407 + TaiI ACGT 407 403 . . 404 407 + SetI ASST 407 403 . . 404 407 + MaeII ACGT 404 406 . . 406 410 + MaeIII GTNAC 405 410 . . 412 415 + MaeI CTAG 412 414 . . 423 426 + SetI ASST 426 422 . . 439 449 + BsiYI* CCNNNNNNNGG 445 442 . . 455 460 + AflIII ACRYGT 455 459 . . 457 460 + TaiI ACGT 460 456 . . 457 460 + SetI ASST 460 456 . . 457 460 + MaeII ACGT 457 459 . . 468 471 + CviRI* TGCA 469 469 . . 472 476 + Tsp4CI* ACNGT 474 473 . . 481 485 + TfiI GAWTC 481 484 . . 481 485 + HinfI GANTC 481 484 . . 486 489 + CviRI* TGCA 487 487 . . 489 492 + TspEI AATT 488 492 . . 510 515 - BsrDI GCAATG 509 507 . . 517 522 + AsuII TTCGAA 518 520 . . 518 521 + TaqI TCGA 518 520 . . 519 530 + BcgI CGANNNNNNTGC 508 506 542 540 509 520 - BcgI CGANNNNNNTGC 532 530 498 496 527 532 + Hpy166II GTNNAC 529 529 . . 527 532 + SduI GDGCHC 531 527 . . 527 532 + BseSI GKGCMC 531 527 . . 527 532 + ApaLI GTGCAC 527 531 . . 527 532 + HgiAI* GWGCWC 531 527 . . 528 531 + CviRI* TGCA 529 529 . . 540 543 + SetI ASST 543 539 . . 541 544 + MnlI CCTC 551 550 . . 545 549 + BccI CCATC 553 554 . . 549 554 + BtrI CACGTC 551 551 . . 550 553 + TaiI ACGT 553 549 . . 550 553 + SetI ASST 553 549 . . 550 553 + MaeII ACGT 550 552 . . 551 555 + Hpy99I CGWCG 555 550 . . 552 557 + SalI GTCGAC 552 556 . . 552 557 + Hpy166II GTNNAC 554 554 . . 552 557 + HindII GTYRAC 554 554 . . 552 557 + AccI GTMKAC 553 555 . . 553 556 + TaqI TCGA 553 555 . . 581 584 + CviJI RGCY 582 582 . . 583 588 + DsaI* CCRYGG 583 587 . . 583 588 + StyI CCWWGG 583 587 . . 583 588 + NcoI CCATGG 583 587 . . 583 588 + SecI* CCNNGG 583 587 . . 583 593 + BsiYI* CCNNNNNNNGG 589 586 . . 583 593 + PflMI CCANNNNNTGG 589 586 . . 584 587 + FatI CATG 583 587 . . 584 587 + NlaIII CATG 587 583 . . 584 587 + CviAII CATG 584 586 . . 588 592 + FokI GGATG 601 605 . . 588 592 + BseGI GGATG 594 592 . . 589 593 - BccI CCATC 583 584 . . 596 600 + BsmAI GTCTC 601 605 . . 614 622 + AlwNI CAGNNNCTG 619 616 . . 616 620 + BslFI GGGAC 630 634 . . 619 623 + Tsp4CI* ACNGT 621 620 . . 622 627 + Hpy166II GTNNAC 624 624 . . 622 627 + AccI GTMKAC 623 625 . . 626 631 + BspLU11I* ACATGT 626 630 . . 626 631 + NspI RCATGY 630 626 . . 626 631 + AflIII ACRYGT 626 630 . . 627 630 + FatI CATG 626 630 . . 627 630 + NlaIII CATG 630 626 . . 627 630 + CviAII CATG 627 629 . . 633 636 + CviRI* TGCA 634 634 . . 634 639 + Cac8I GCNNGC 636 636 . . 637 640 + CviJI RGCY 638 638 . . 649 654 + NspBII* CMGCKG 651 651 . . 649 652 + AciI CCGC 649 651 . . 652 657 + Eco57MI CTGRAG 673 671 . . 652 657 + Eco57I CTGAAG 673 671 . . 654 666 + BarI GAAGNNNNNNTAC 646 641 678 673 656 659 + SetI ASST 659 655 . . 654 658 - Hin4II* CCTTC 648 647 . . 661 666 + Hpy166II GTNNAC 663 663 . . 674 684 + BsiYI* CCNNNNNNNGG 680 677 . . 673 677 - BceAI ACGGC 658 660 . . 680 684 + BssKI CCNGG 679 684 . . 680 684 + BseBI CCWGG 681 682 . . 680 685 + SecI* CCNNGG 680 684 . . 680 684 + ScrFI CCNGG 681 682 . . 680 684 + EcoRII CCWGG 679 684 . . 684 689 + SduI GDGCHC 688 684 . . 684 689 + BseSI GKGCMC 688 684 . . 686 691 + Cac8I GCNNGC 688 688 . . 695 706 + CspCI CAANNNNNGTGG 683 681 718 716 696 699 + TspEI AATT 695 699 . . 707 710 + TspEI AATT 706 710 . . 719 722 + Csp6I GTAC 719 721 . . 715 719 - BccI CCATC 709 710 . . 719 722 + RsaI GTAC 720 720 . . 724 728 + Tsp4CI* ACNGT 726 725 . . 718 728 - BaeI ACNNNNGTAYC 743 738 710 705 742 746 - Hin4II* CCTTC 736 735 . . 747 750 + Csp6I GTAC 747 749 . . 747 750 + RsaI GTAC 748 748 . . 749 752 + TaiI ACGT 752 748 . . 749 752 + SetI ASST 752 748 . . 749 752 + MaeII ACGT 749 751 . . 751 756 + Hpy166II GTNNAC 753 753 . . 768 779 + BdaI TGANNNNNNTCA 757 755 791 789 769 778 + PshAI GACNNNNGTC 773 773 . . 770 773 + SetI ASST 773 769 . . 772 776 + DdeI CTNAG 772 775 . . 768 779 - BdaI TGANNNNNNTCA 791 789 757 755 784 787 + TspEI AATT 783 787 . . 787 792 + TatI WGTACW 787 791 . . 788 791 + Csp6I GTAC 788 790 . . 788 791 + RsaI GTAC 789 789 . . 799 802 + DpnI GATC 800 800 . . 799 802 + BstKTI GATC 801 799 . . 799 802 + MboI GATC 798 802 . . 802 806 + Hin4II* CCTTC 812 811 . . 799 803 - BinI* GGATC 793 794 . . 818 821 + MaeI CTAG 818 820 . . 839 849 + BsiYI* CCNNNNNNNGG 845 842 . . 842 845 + CviJI RGCY 843 843 . . 844 849 + DsaI* CCRYGG 844 848 . . 844 849 + StyI CCWWGG 844 848 . . 844 849 + NcoI CCATGG 844 848 . . 844 849 + SecI* CCNNGG 844 848 . . 844 854 + BsiYI* CCNNNNNNNGG 850 847 . . 845 848 + FatI CATG 844 848 . . 845 848 + NlaIII CATG 848 844 . . 845 848 + CviAII CATG 845 847 . . 848 852 + BmgT120I GGNCC 849 850 . . 848 852 + AvaII GGWCC 848 851 . . 848 852 + AsuI* GGNCC 848 851 . . 850 855 + Cfr10I RCCGGY 850 854 . . 850 855 + BetI* WCCGGW 850 854 . . 850 855 + AgeI ACCGGT 850 854 . . 851 854 + HpaII CCGG 851 853 . . 854 857 + Csp6I GTAC 854 856 . . 854 857 + RsaI GTAC 855 855 . . 854 866 - BarI GAAGNNNNNNTAC 878 873 846 841 873 879 + TspRI CASTGNN 879 870 . . 873 882 + MslI CAYNNNNRTG 877 877 . . 869 873 - FokI GGATG 855 859 . . 869 873 - BseGI GGATG 868 866 . . 875 879 + DdeI CTNAG 875 878 . . 875 879 - BspCNI CTCAG 867 865 . . 875 879 - BseMII CTCAG 866 864 . . 881 892 + BdaI TGANNNNNNTCA 870 868 904 902 887 891 + BccI CCATC 895 896 . . 881 892 - BdaI TGANNNNNNTCA 904 902 870 868 890 894 - HphI GGTGA 882 881 . . 902 906 + Tsp4CI* ACNGT 904 903 . . 908 911 + TspEI AATT 907 911 . . 910 913 + MseI TTAA 910 912 . . 917 921 + TspDTI ATGAA 932 930 . . 922 926 + Tsp4CI* ACNGT 924 923 . . 935 939 + Hpy188I TCNGA 937 936 . . 942 947 + MmeI TCCRAC 967 965 . . 951 954 + SetI ASST 954 950 . . 951 954 + CviJI RGCY 952 952 . . 951 954 + AluI AGCT 952 952 . . 952 957 + Cac8I GCNNGC 954 954 . . 952 957 + BmtI GCTAGC 956 952 . . 952 957 + NheI GCTAGC 952 956 . . 953 956 + MaeI CTAG 953 955 . . 959 963 + BccI CCATC 967 968 . . 974 984 + BsiYI* CCNNNNNNNGG 980 977 . . 977 980 + CviJI RGCY 978 978 . . 979 984 + DsaI* CCRYGG 979 983 . . 979 984 + StyI CCWWGG 979 983 . . 979 984 + NcoI CCATGG 979 983 . . 979 984 + SecI* CCNNGG 979 983 . . 979 989 + BsiYI* CCNNNNNNNGG 985 982 . . 980 983 + FatI CATG 979 983 . . 980 983 + NlaIII CATG 983 979 . . 980 983 + CviAII CATG 980 982 . . 983 987 + BmgT120I GGNCC 984 985 . . 983 987 + AvaII GGWCC 983 986 . . 983 987 + AsuI* GGNCC 983 986 . . 985 990 + Cfr10I RCCGGY 985 989 . . 985 990 + BetI* WCCGGW 985 989 . . 985 990 + AgeI ACCGGT 985 989 . . 986 989 + HpaII CCGG 986 988 . . 989 992 + Csp6I GTAC 989 991 . . 989 992 + RsaI GTAC 990 990 . . 997 1000 + SetI ASST 1000 996 . . 998 1001 + MnlI CCTC 1008 1007 . . 995 999 - HphI GGTGA 987 986 . . 1008 1014 + TspRI CASTGNN 1014 1005 . . 1008 1017 + MslI CAYNNNNRTG 1012 1012 . . 1004 1008 - FokI GGATG 990 994 . . 1004 1008 - BseGI GGATG 1003 1001 . . 1010 1014 + DdeI CTNAG 1010 1013 . . 1010 1014 - BspCNI CTCAG 1002 1000 . . 1010 1014 - BseMII CTCAG 1001 999 . . 1016 1027 + BdaI TGANNNNNNTCA 1005 1003 1039 1037 1017 1026 + PshAI GACNNNNGTC 1021 1021 . . 1021 1025 + Tsp4CI* ACNGT 1023 1022 . . 1024 1028 + Tsp45I GTSAC 1023 1028 . . 1024 1028 + MaeIII GTNAC 1023 1028 . . 1027 1032 + Cfr10I RCCGGY 1027 1031 . . 1027 1032 + BetI* WCCGGW 1027 1031 . . 1016 1027 - BdaI TGANNNNNNTCA 1039 1037 1005 1003 1027 1032 + AgeI ACCGGT 1027 1031 . . 1028 1031 + HpaII CCGG 1028 1030 . . 1025 1029 - HphI GGTGA 1017 1016 . . 1030 1035 + KpnI GGTACC 1034 1030 . . 1030 1035 + NlaIV GGNNCC 1032 1032 . . 1030 1035 + HgiCI* GGYRCC 1030 1034 . . 1030 1035 + Acc65I GGTACC 1030 1034 . . 1031 1034 + Csp6I GTAC 1031 1033 . . 1031 1034 + RsaI GTAC 1032 1032 . . 1037 1041 + Tsp4CI* ACNGT 1039 1038 . . 1040 1045 + Hpy166II GTNNAC 1042 1042 . . 1040 1045 + HindII GTYRAC 1042 1042 . . 1057 1061 + Tsp4CI* ACNGT 1059 1058 . . 1077 1082 + MmeI TCCRAC 1102 1100 . . 1081 1086 + SpeI ACTAGT 1081 1085 . . 1082 1085 + MaeI CTAG 1082 1084 . . 1087 1090 - MnlI CCTC 1080 1079 . . 1109 1119 + BsiYI* CCNNNNNNNGG 1115 1112 . . 1114 1119 + DsaI* CCRYGG 1114 1118 . . 1114 1119 + StyI CCWWGG 1114 1118 . . 1114 1119 + NcoI CCATGG 1114 1118 . . 1114 1119 + SecI* CCNNGG 1114 1118 . . 1114 1124 + BsiYI* CCNNNNNNNGG 1120 1117 . . 1115 1118 + FatI CATG 1114 1118 . . 1115 1118 + NlaIII CATG 1118 1114 . . 1115 1118 + CviAII CATG 1115 1117 . . 1118 1122 + BmgT120I GGNCC 1119 1120 . . 1118 1122 + AvaII GGWCC 1118 1121 . . 1118 1122 + AsuI* GGNCC 1118 1121 . . 1120 1125 + Cfr10I RCCGGY 1120 1124 . . 1120 1125 + BetI* WCCGGW 1120 1124 . . 1120 1125 + AgeI ACCGGT 1120 1124 . . 1121 1124 + HpaII CCGG 1121 1123 . . 1124 1127 + Csp6I GTAC 1124 1126 . . 1124 1127 + RsaI GTAC 1125 1125 . . 1132 1135 + SetI ASST 1135 1131 . . 1133 1136 + MnlI CCTC 1143 1142 . . 1130 1134 - HphI GGTGA 1122 1121 . . 1143 1149 + TspRI CASTGNN 1149 1140 . . 1143 1152 + MslI CAYNNNNRTG 1147 1147 . . 1139 1143 - FokI GGATG 1125 1129 . . 1139 1143 - BseGI GGATG 1138 1136 . . 1145 1149 + DdeI CTNAG 1145 1148 . . 1145 1149 - BspCNI CTCAG 1137 1135 . . 1145 1149 - BseMII CTCAG 1136 1134 . . 1156 1160 + Tsp4CI* ACNGT 1158 1157 . . 1159 1163 + Tsp45I GTSAC 1158 1163 . . 1159 1163 + MaeIII GTNAC 1158 1163 . . 1162 1167 + Cfr10I RCCGGY 1162 1166 . . 1162 1167 + BetI* WCCGGW 1162 1166 . . 1162 1167 + AgeI ACCGGT 1162 1166 . . 1163 1166 + HpaII CCGG 1163 1165 . . 1165 1170 + KpnI GGTACC 1169 1165 . . 1165 1170 + NlaIV GGNNCC 1167 1167 . . 1165 1170 + HgiCI* GGYRCC 1165 1169 . . 1165 1170 + Acc65I GGTACC 1165 1169 . . 1166 1169 + Csp6I GTAC 1166 1168 . . 1166 1169 + RsaI GTAC 1167 1167 . . 1172 1176 + Tsp4CI* ACNGT 1174 1173 . . 1175 1180 + Hpy166II GTNNAC 1177 1177 . . 1175 1180 + HindII GTYRAC 1177 1177 . . 1192 1196 + Tsp4CI* ACNGT 1194 1193 . . 1212 1217 + MmeI TCCRAC 1237 1235 . . 1216 1221 + SpeI ACTAGT 1216 1220 . . 1217 1220 + MaeI CTAG 1217 1219 . . 1222 1225 - MnlI CCTC 1215 1214 . . 1244 1254 + BsiYI* CCNNNNNNNGG 1250 1247 . . 1249 1254 + DsaI* CCRYGG 1249 1253 . . 1249 1254 + StyI CCWWGG 1249 1253 . . 1249 1254 + NcoI CCATGG 1249 1253 . . 1249 1254 + SecI* CCNNGG 1249 1253 . . 1249 1259 + BsiYI* CCNNNNNNNGG 1255 1252 . . 1250 1253 + FatI CATG 1249 1253 . . 1250 1253 + NlaIII CATG 1253 1249 . . 1250 1253 + CviAII CATG 1250 1252 . . 1253 1257 + BmgT120I GGNCC 1254 1255 . . 1253 1257 + AvaII GGWCC 1253 1256 . . 1253 1257 + AsuI* GGNCC 1253 1256 . . 1255 1260 + Cfr10I RCCGGY 1255 1259 . . 1255 1260 + BetI* WCCGGW 1255 1259 . . 1255 1260 + AgeI ACCGGT 1255 1259 . . 1256 1259 + HpaII CCGG 1256 1258 . . 1259 1262 + Csp6I GTAC 1259 1261 . . 1259 1262 + RsaI GTAC 1260 1260 . . 1267 1270 + SetI ASST 1270 1266 . . 1268 1271 + MnlI CCTC 1278 1277 . . 1265 1269 - HphI GGTGA 1257 1256 . . 1278 1284 + TspRI CASTGNN 1284 1275 . . 1274 1278 - FokI GGATG 1260 1264 . . 1274 1278 - BseGI GGATG 1273 1271 . . 1280 1284 + DdeI CTNAG 1280 1283 . . 1283 1286 + SetI ASST 1286 1282 . . 1284 1290 + BstEII GGTNACC 1284 1289 . . 1280 1284 - BspCNI CTCAG 1272 1270 . . 1280 1284 - BseMII CTCAG 1271 1269 . . 1282 1285 - MnlI CCTC 1275 1274 . . 1285 1289 + MaeIII GTNAC 1284 1289 . . 1292 1296 + BccI CCATC 1300 1301 . . 1296 1302 + TspRI CASTGNN 1302 1293 . . 1297 1301 + BsrI ACTGG 1302 1300 . . 1300 1305 + KpnI GGTACC 1304 1300 . . 1300 1305 + NlaIV GGNNCC 1302 1302 . . 1300 1305 + HgiCI* GGYRCC 1300 1304 . . 1300 1305 + Acc65I GGTACC 1300 1304 . . 1301 1304 + Csp6I GTAC 1301 1303 . . 1301 1304 + RsaI GTAC 1302 1302 . . 1307 1311 + Tsp4CI* ACNGT 1309 1308 . . 1310 1315 + Hpy166II GTNNAC 1312 1312 . . 1310 1315 + HindII GTYRAC 1312 1312 . . 1327 1331 + Tsp4CI* ACNGT 1329 1328 . . 1347 1352 + MmeI TCCRAC 1372 1370 . . 1351 1356 + SpeI ACTAGT 1351 1355 . . 1352 1355 + MaeI CTAG 1352 1354 . . 1357 1360 - MnlI CCTC 1350 1349 . . 1379 1389 + BsiYI* CCNNNNNNNGG 1385 1382 . . 1384 1389 + DsaI* CCRYGG 1384 1388 . . 1384 1389 + StyI CCWWGG 1384 1388 . . 1384 1389 + NcoI CCATGG 1384 1388 . . 1384 1389 + SecI* CCNNGG 1384 1388 . . 1384 1394 + BsiYI* CCNNNNNNNGG 1390 1387 . . 1385 1388 + FatI CATG 1384 1388 . . 1385 1388 + NlaIII CATG 1388 1384 . . 1385 1388 + CviAII CATG 1385 1387 . . 1388 1392 + BmgT120I GGNCC 1389 1390 . . 1388 1392 + AvaII GGWCC 1388 1391 . . 1388 1392 + AsuI* GGNCC 1388 1391 . . 1390 1395 + Cfr10I RCCGGY 1390 1394 . . 1390 1395 + BetI* WCCGGW 1390 1394 . . 1390 1395 + AgeI ACCGGT 1390 1394 . . 1391 1394 + HpaII CCGG 1391 1393 . . 1394 1397 + Csp6I GTAC 1394 1396 . . 1394 1397 + RsaI GTAC 1395 1395 . . 1402 1405 + SetI ASST 1405 1401 . . 1403 1406 + MnlI CCTC 1413 1412 . . 1400 1404 - HphI GGTGA 1392 1391 . . 1415 1419 + DdeI CTNAG 1415 1418 . . 1415 1419 - BspCNI CTCAG 1407 1405 . . 1415 1419 - BseMII CTCAG 1406 1404 . . 1421 1432 + BdaI TGANNNNNNTCA 1410 1408 1444 1442 1422 1431 + PshAI GACNNNNGTC 1426 1426 . . 1426 1430 + Tsp4CI* ACNGT 1428 1427 . . 1429 1433 + Tsp45I GTSAC 1428 1433 . . 1429 1433 + MaeIII GTNAC 1428 1433 . . 1432 1437 + Cfr10I RCCGGY 1432 1436 . . 1432 1437 + BetI* WCCGGW 1432 1436 . . 1421 1432 - BdaI TGANNNNNNTCA 1444 1442 1410 1408 1432 1437 + AgeI ACCGGT 1432 1436 . . 1433 1436 + HpaII CCGG 1433 1435 . . 1430 1434 - HphI GGTGA 1422 1421 . . 1435 1440 + KpnI GGTACC 1439 1435 . . 1435 1440 + NlaIV GGNNCC 1437 1437 . . 1435 1440 + HgiCI* GGYRCC 1435 1439 . . 1435 1440 + Acc65I GGTACC 1435 1439 . . 1436 1439 + Csp6I GTAC 1436 1438 . . 1436 1439 + RsaI GTAC 1437 1437 . . 1442 1446 + Tsp4CI* ACNGT 1444 1443 . . 1445 1450 + Hpy166II GTNNAC 1447 1447 . . 1445 1450 + HindII GTYRAC 1447 1447 . . 1462 1466 + Tsp4CI* ACNGT 1464 1463 . . 1475 1479 + Hpy188I TCNGA 1477 1476 . . 1482 1487 + MmeI TCCRAC 1507 1505 . . 1488 1494 + TspRI CASTGNN 1494 1485 . . 1487 1491 - BsrI ACTGG 1487 1485 . . 1494 1497 + SetI ASST 1497 1493 . . 1492 1496 - Hin4II* CCTTC 1486 1485 . . 1512 1518 + TspRI CASTGNN 1518 1509 . . 1519 1524 + DsaI* CCRYGG 1519 1523 . . 1519 1524 + StyI CCWWGG 1519 1523 . . 1519 1524 + NcoI CCATGG 1519 1523 . . 1519 1524 + SecI* CCNNGG 1519 1523 . . 1519 1529 + BsiYI* CCNNNNNNNGG 1525 1522 . . 1519 1529 + PflMI CCANNNNNTGG 1525 1522 . . 1520 1523 + FatI CATG 1519 1523 . . 1520 1523 + NlaIII CATG 1523 1519 . . 1520 1523 + CviAII CATG 1520 1522 . . 1525 1529 + BsrI ACTGG 1530 1528 . . 1529 1532 + Csp6I GTAC 1529 1531 . . 1529 1532 + RsaI GTAC 1530 1530 . . 1537 1540 + SetI ASST 1540 1536 . . 1538 1541 + MnlI CCTC 1548 1547 . . 1535 1539 - HphI GGTGA 1527 1526 . . 1550 1554 + DdeI CTNAG 1550 1553 . . 1553 1556 + SetI ASST 1556 1552 . . 1554 1560 + BstEII GGTNACC 1554 1559 . . 1550 1554 - BspCNI CTCAG 1542 1540 . . 1550 1554 - BseMII CTCAG 1541 1539 . . 1552 1555 - MnlI CCTC 1545 1544 . . 1555 1559 + MaeIII GTNAC 1554 1559 . . 1562 1566 + BccI CCATC 1570 1571 . . 1566 1572 + TspRI CASTGNN 1572 1563 . . 1567 1571 + BsrI ACTGG 1572 1570 . . 1570 1575 + KpnI GGTACC 1574 1570 . . 1570 1575 + NlaIV GGNNCC 1572 1572 . . 1570 1575 + HgiCI* GGYRCC 1570 1574 . . 1570 1575 + Acc65I GGTACC 1570 1574 . . 1571 1574 + Csp6I GTAC 1571 1573 . . 1571 1574 + RsaI GTAC 1572 1572 . . 1577 1581 + Tsp4CI* ACNGT 1579 1578 . . 1580 1585 + Hpy166II GTNNAC 1582 1582 . . 1580 1585 + HindII GTYRAC 1582 1582 . . 1597 1601 + Tsp4CI* ACNGT 1599 1598 . . 1617 1622 + MmeI TCCRAC 1642 1640 . . 1621 1626 + SpeI ACTAGT 1621 1625 . . 1622 1625 + MaeI CTAG 1622 1624 . . 1627 1630 - MnlI CCTC 1620 1619 . . 1649 1659 + BsiYI* CCNNNNNNNGG 1655 1652 . . 1654 1659 + DsaI* CCRYGG 1654 1658 . . 1654 1659 + StyI CCWWGG 1654 1658 . . 1654 1659 + NcoI CCATGG 1654 1658 . . 1654 1659 + SecI* CCNNGG 1654 1658 . . 1654 1664 + BsiYI* CCNNNNNNNGG 1660 1657 . . 1655 1658 + FatI CATG 1654 1658 . . 1655 1658 + NlaIII CATG 1658 1654 . . 1655 1658 + CviAII CATG 1655 1657 . . 1658 1662 + BmgT120I GGNCC 1659 1660 . . 1658 1662 + AvaII GGWCC 1658 1661 . . 1658 1662 + AsuI* GGNCC 1658 1661 . . 1660 1665 + BetI* WCCGGW 1660 1664 . . 1661 1664 + HpaII CCGG 1661 1663 . . 1672 1675 + SetI ASST 1675 1671 . . 1673 1676 + MnlI CCTC 1683 1682 . . 1670 1674 - HphI GGTGA 1662 1661 . . 1683 1689 + TspRI CASTGNN 1689 1680 . . 1683 1692 + MslI CAYNNNNRTG 1687 1687 . . 1679 1683 - FokI GGATG 1665 1669 . . 1679 1683 - BseGI GGATG 1678 1676 . . 1685 1689 + DdeI CTNAG 1685 1688 . . 1685 1689 - BspCNI CTCAG 1677 1675 . . 1685 1689 - BseMII CTCAG 1676 1674 . . 1696 1700 + Tsp4CI* ACNGT 1698 1697 . . 1699 1703 + Tsp45I GTSAC 1698 1703 . . 1699 1703 + MaeIII GTNAC 1698 1703 . . 1702 1707 + Cfr10I RCCGGY 1702 1706 . . 1702 1707 + BetI* WCCGGW 1702 1706 . . 1702 1707 + AgeI ACCGGT 1702 1706 . . 1703 1706 + HpaII CCGG 1703 1705 . . 1705 1710 + KpnI GGTACC 1709 1705 . . 1705 1710 + NlaIV GGNNCC 1707 1707 . . 1705 1710 + HgiCI* GGYRCC 1705 1709 . . 1705 1710 + Acc65I GGTACC 1705 1709 . . 1706 1709 + Csp6I GTAC 1706 1708 . . 1706 1709 + RsaI GTAC 1707 1707 . . 1712 1716 + Tsp4CI* ACNGT 1714 1713 . . 1715 1720 + Hpy166II GTNNAC 1717 1717 . . 1715 1720 + HindII GTYRAC 1717 1717 . . 1732 1736 + Tsp4CI* ACNGT 1734 1733 . . 1752 1757 + MmeI TCCRAC 1777 1775 . . 1756 1761 + SpeI ACTAGT 1756 1760 . . 1757 1760 + MaeI CTAG 1757 1759 . . 1762 1765 - MnlI CCTC 1755 1754 . . 1775 1778 + MaeI CTAG 1775 1777 . . 1784 1794 + BsiYI* CCNNNNNNNGG 1790 1787 . . 1789 1794 + DsaI* CCRYGG 1789 1793 . . 1789 1794 + StyI CCWWGG 1789 1793 . . 1789 1794 + NcoI CCATGG 1789 1793 . . 1789 1794 + SecI* CCNNGG 1789 1793 . . 1789 1799 + BsiYI* CCNNNNNNNGG 1795 1792 . . 1789 1799 + PflMI CCANNNNNTGG 1795 1792 . . 1790 1793 + FatI CATG 1789 1793 . . 1790 1793 + NlaIII CATG 1793 1789 . . 1790 1793 + CviAII CATG 1790 1792 . . 1795 1799 + BsrI ACTGG 1800 1798 . . 1799 1802 + Csp6I GTAC 1799 1801 . . 1799 1802 + RsaI GTAC 1800 1800 . . 1799 1811 - BarI GAAGNNNNNNTAC 1823 1818 1791 1786 1820 1824 + DdeI CTNAG 1820 1823 . . 1823 1826 + SetI ASST 1826 1822 . . 1824 1830 + BstEII GGTNACC 1824 1829 . . 1820 1824 - BspCNI CTCAG 1812 1810 . . 1820 1824 - BseMII CTCAG 1811 1809 . . 1822 1825 - MnlI CCTC 1815 1814 . . 1825 1829 + MaeIII GTNAC 1824 1829 . . 1832 1836 + BccI CCATC 1840 1841 . . 1837 1842 + Cfr10I RCCGGY 1837 1841 . . 1837 1842 + BetI* WCCGGW 1837 1841 . . 1837 1842 + AgeI ACCGGT 1837 1841 . . 1838 1841 + HpaII CCGG 1838 1840 . . 1835 1839 - HphI GGTGA 1827 1826 . . 1840 1845 + KpnI GGTACC 1844 1840 . . 1840 1845 + NlaIV GGNNCC 1842 1842 . . 1840 1845 + HgiCI* GGYRCC 1840 1844 . . 1840 1845 + Acc65I GGTACC 1840 1844 . . 1841 1844 + Csp6I GTAC 1841 1843 . . 1841 1844 + RsaI GTAC 1842 1842 . . 1847 1851 + Tsp4CI* ACNGT 1849 1848 . . 1850 1855 + Hpy166II GTNNAC 1852 1852 . . 1850 1855 + HindII GTYRAC 1852 1852 . . 1867 1871 + Tsp4CI* ACNGT 1869 1868 . . 1880 1884 + Hpy188I TCNGA 1882 1881 . . 1883 1895 + PsrI GAACNNNNNNTAC 1875 1870 1907 1902 1887 1892 + MmeI TCCRAC 1912 1910 . . 1898 1902 + BccI CCATC 1906 1907 . . 1904 1908 - FokI GGATG 1890 1894 . . 1904 1908 - BseGI GGATG 1903 1901 . . 1907 1911 - BsrI ACTGG 1907 1905 . . 1918 1922 - MboII GAAGA 1910 1909 . . 1924 1929 + Hpy178III* TCNNGA 1925 1927 . . 1921 1925 - MboII GAAGA 1913 1912 . . 1922 1927 - Eco57MI CTGRAG 1907 1905 . . 1922 1927 - Eco57I CTGAAG 1907 1905 . . 1934 1938 - HphI GGTGA 1926 1925 . . 1939 1942 + SetI ASST 1942 1938 . . 1939 1942 + CviJI RGCY 1940 1940 . . 1939 1942 + AluI AGCT 1940 1940 . . 1947 1952 + BtrI CACGTC 1949 1949 . . 1948 1951 + TaiI ACGT 1951 1947 . . 1948 1951 + SetI ASST 1951 1947 . . 1948 1951 + MaeII ACGT 1948 1950 . . 1951 1955 - MboII GAAGA 1943 1942 . . 1950 1955 - BbvII* GAAGAC 1943 1947 . . 1955 1958 + FnuDII* CGCG 1956 1956 . . 1956 1960 - HgaI GACGC 1945 1950 . . 1962 1965 + TspEI AATT 1961 1965 . . 1981 1991 + BsiYI* CCNNNNNNNGG 1987 1984 . . 1982 1985 + MaeI CTAG 1982 1984 . . 1985 1990 + BsrDI GCAATG 1992 1990 . . 1990 2001 - TstI CACNNNNNNTCC 2014 2009 1982 1977 2006 2009 + MnlI CCTC 2016 2015 . . 2009 2012 + MnlI CCTC 2019 2018 . . 2010 2014 + DdeI CTNAG 2010 2013 . . 2010 2014 + BspCNI CTCAG 2023 2021 . . 2010 2014 + BseMII CTCAG 2024 2022 . . 2007 2012 - BseRI GAGGAG 1998 1996 . . 2016 2019 + TspEI AATT 2015 2019 . . 2020 2024 - MboII GAAGA 2012 2011 . . 2023 2027 - MboII GAAGA 2015 2014 . . 2029 2033 + Tsp45I GTSAC 2028 2033 . . 2029 2033 + MaeIII GTNAC 2028 2033 . . 2024 2029 - Eco57MI CTGRAG 2009 2007 . . 2024 2029 - Eco57I CTGAAG 2009 2007 . . 2035 2039 - MboII GAAGA 2027 2026 . . 2041 2044 + MaeI CTAG 2041 2043 . . 2044 2048 + Tsp45I GTSAC 2043 2048 . . 2044 2048 + MaeIII GTNAC 2043 2048 . . 2047 2050 + SetI ASST 2050 2046 . . 2048 2051 + MnlI CCTC 2058 2057 . . 2049 2054 + Ksp632I* CTCTTC 2055 2058 . . 2045 2049 - HphI GGTGA 2037 2036 . . 2050 2054 - MboII GAAGA 2042 2041 . . 2053 2057 - MboII GAAGA 2045 2044 . . 2055 2059 - TspDTI ATGAA 2045 2043 . . 2059 2063 - TspDTI ATGAA 2049 2047 . . 2069 2072 + MnlI CCTC 2079 2078 . . 2065 2069 - MboII GAAGA 2057 2056 . . 2080 2084 - MboII GAAGA 2072 2071 . . 2083 2087 - MboII GAAGA 2075 2074 . . 2095 2098 + SetI ASST 2098 2094 . . 2096 2099 + MnlI CCTC 2106 2105 . . 2113 2117 + Hpy188I TCNGA 2115 2114 . . 2116 2120 + TfiI GAWTC 2116 2119 . . 2116 2120 + HinfI GANTC 2116 2119 . . 2129 2133 - FokI GGATG 2115 2119 . . 2129 2133 - BseGI GGATG 2128 2126 . . 2131 2135 - TspGWI ACGGA 2121 2119 . . 2139 2144 + MmeI TCCRAC 2164 2162 . . 2144 2148 - BsrI ACTGG 2144 2142 . . 2140 2153 - AjuI GAANNNNNNNTTGG 2165 2160 2133 2128 2155 2158 + SetI ASST 2158 2154 . . 2156 2159 + MnlI CCTC 2166 2165 . . 2167 2171 + Hpy188I TCNGA 2169 2168 . . 2164 2168 - MboII GAAGA 2156 2155 . . 2168 2172 + DdeI CTNAG 2168 2171 . . 2168 2172 - BspCNI CTCAG 2160 2158 . . 2168 2172 - BseMII CTCAG 2159 2157 . . 2187 2190 + CviJI RGCY 2188 2188 . . 2189 2192 + MaeI CTAG 2189 2191 . . 2198 2201 + MnlI CCTC 2208 2207 . . 2194 2198 - MboII GAAGA 2186 2185 . . 2199 2204 + Ksp632I* CTCTTC 2205 2208 . . 2200 2204 - MboII GAAGA 2192 2191 . . 2207 2210 + MnlI CCTC 2217 2216 . . 2203 2207 - MboII GAAGA 2195 2194 . . 2208 2213 + Ksp632I* CTCTTC 2214 2217 . . 2209 2213 - MboII GAAGA 2201 2200 . . 2217 2222 + Ksp632I* CTCTTC 2223 2226 . . 2221 2225 + Hpy188I TCNGA 2223 2222 . . 2218 2222 - MboII GAAGA 2210 2209 . . 2224 2228 + TfiI GAWTC 2224 2227 . . 2224 2228 + HinfI GANTC 2224 2227 . . 2227 2231 - HphI GGTGA 2219 2218 . . 2235 2239 + BsmAI GTCTC 2240 2244 . . 2244 2249 + ApoI RAATTY 2244 2248 . . 2245 2248 + TspEI AATT 2244 2248 . . 2248 2252 - MboII GAAGA 2240 2239 . . 2251 2255 - MboII GAAGA 2243 2242 . . 2253 2257 - TspDTI ATGAA 2243 2241 . . 2265 2268 + SetI ASST 2268 2264 . . 2269 2273 + MaeIII GTNAC 2268 2273 . . 2274 2280 + TspRI CASTGNN 2280 2271 . . 2273 2277 - BsrI ACTGG 2273 2271 . . 2287 2290 + CviJI RGCY 2288 2288 . . 2287 2290 + HaeIII GGCC 2288 2288 . . 2289 2293 + BssKI CCNGG 2288 2293 . . 2289 2293 + BseBI CCWGG 2290 2291 . . 2289 2293 + ScrFI CCNGG 2290 2291 . . 2289 2293 + EcoRII CCWGG 2288 2293 . . 2302 2306 - MboII GAAGA 2294 2293 . . 2304 2308 - TspDTI ATGAA 2294 2292 . . 2312 2318 + AarI CACCTGC 2322 2326 . . 2313 2316 + SetI ASST 2316 2312 . . 2313 2318 + BspMI ACCTGC 2322 2326 . . 2352 2358 + BstEII GGTNACC 2352 2357 . . 2353 2357 + MaeIII GTNAC 2352 2357 . . 2351 2356 - TsoI TARCCA 2341 2339 . . 2356 2360 + Tsp4CI* ACNGT 2358 2357 . . 2359 2363 + Tsp45I GTSAC 2358 2363 . . 2359 2363 + MaeIII GTNAC 2358 2363 . . 2363 2367 - FokI GGATG 2349 2353 . . 2363 2367 - BseGI GGATG 2362 2360 . . 2371 2375 + TfiI GAWTC 2371 2374 . . 2371 2375 + HinfI GANTC 2371 2374 . . 2377 2380 + FatI CATG 2376 2380 . . 2377 2380 + NlaIII CATG 2380 2376 . . 2377 2380 + CviAII CATG 2377 2379 . . 2382 2387 + Hpy166II GTNNAC 2384 2384 . . 2383 2388 + TatI WGTACW 2383 2387 . . 2384 2387 + Csp6I GTAC 2384 2386 . . 2384 2387 + RsaI GTAC 2385 2385 . . 2389 2393 + TfiI GAWTC 2389 2392 . . 2389 2393 + HinfI GANTC 2389 2392 . . 2393 2397 + BccI CCATC 2401 2402 . . 2399 2402 + MnlI CCTC 2409 2408 . . 2397 2402 - BseRI GAGGAG 2388 2386 . . 2414 2419 + DsaI* CCRYGG 2414 2418 . . 2414 2419 + SecI* CCNNGG 2414 2418 . . 2416 2420 + BceAI ACGGC 2432 2434 . . 2417 2422 + CfrI YGGCCR 2417 2421 . . 2418 2421 + CviJI RGCY 2419 2419 . . 2418 2421 + HaeIII GGCC 2419 2419 . . 2422 2426 + Tsp4CI* ACNGT 2424 2423 . . 2425 2429 + MaeIII GTNAC 2424 2429 . . 2428 2432 + Tsp4CI* ACNGT 2430 2429 . . 2435 2439 + TauI GCSGC 2438 2435 . . 2435 2439 + BlsI GCNGC 2437 2436 . . 2435 2439 + BisI GCNGC 2436 2437 . . 2437 2442 + AcyI GRCGYC 2438 2440 . . 2435 2438 - AciI CCGC 2435 2437 . . 2440 2444 + Tsp45I GTSAC 2439 2444 . . 2440 2444 + MaeIII GTNAC 2439 2444 . . 2438 2442 - HgaI GACGC 2427 2432 . . 2451 2456 + Hpy166II GTNNAC 2453 2453 . . 2451 2456 + BssNAI GTATAC 2453 2453 . . 2451 2456 + AccI GTMKAC 2452 2454 . . 2457 2462 + BsaAI YACGTR 2459 2459 . . 2457 2465 + DraIII CACNNNGTG 2462 2459 . . 2457 2462 + PmaCI CACGTG 2459 2459 . . 2458 2461 + TaiI ACGT 2461 2457 . . 2458 2461 + SetI ASST 2461 2457 . . 2458 2461 + MaeII ACGT 2458 2460 . . 2462 2467 + NlaIV GGNNCC 2464 2464 . . 2462 2467 + HgiCI* GGYRCC 2462 2466 . . 2463 2468 + SduI GDGCHC 2467 2463 . . 2463 2468 + BseSI GKGCMC 2467 2463 . . 2482 2486 - BsmAI GTCTC 2476 2480 . . 2498 2503 + StyI CCWWGG 2498 2502 . . 2498 2503 + SecI* CCNNGG 2498 2502 . . 2503 2507 + BslFI GGGAC 2517 2521 . . 2519 2524 + StyI CCWWGG 2519 2523 . . 2519 2524 + SecI* CCNNGG 2519 2523 . . 2524 2528 + BslFI GGGAC 2538 2542 . . 2563 2567 + Tsp4CI* ACNGT 2565 2564 . . 2569 2573 + MaeIII GTNAC 2568 2573 . . 2574 2577 + TspEI AATT 2573 2577 . . 2578 2582 - MboII GAAGA 2570 2569 . . 2587 2591 + TfiI GAWTC 2587 2590 . . 2587 2591 + HinfI GANTC 2587 2590 . . 2590 2594 + Hpy188I TCNGA 2592 2591 . . 2593 2603 + Hin4I GAYNNNNNVTC 2584 2579 2616 2611 2595 2600 + NdeI CATATG 2596 2598 . . 2603 2607 + DdeI CTNAG 2603 2606 . . 2607 2617 + Hin4I GAYNNNNNVTC 2598 2593 2630 2625 2608 2618 + BsaXI ACNNNNNCTCC 2598 2595 2628 2625 2611 2621 + MwoI GCNNNNNNNGC 2617 2614 . . 2619 2622 + CviJI RGCY 2620 2620 . . 2615 2620 - Eco57MI CTGRAG 2600 2598 . . 2615 2620 - GsuI CTGGAG 2600 2598 . . 2628 2633 + Hpy166II GTNNAC 2630 2630 . . 2628 2633 + AccI GTMKAC 2629 2631 . . 2637 2643 + TspRI CASTGNN 2643 2634 . . 2637 2642 - BtsI GCAGTG 2636 2634 . . 2648 2651 + MseI TTAA 2648 2650 . . 2651 2655 + BceAI ACGGC 2667 2669 . . 2656 2660 + MaeIII GTNAC 2655 2660 . . 2675 2678 + FatI CATG 2674 2678 . . 2675 2678 + NlaIII CATG 2678 2674 . . 2675 2678 + CviAII CATG 2675 2677 . . 2698 2702 + TfiI GAWTC 2698 2701 . . 2698 2702 + HinfI GANTC 2698 2701 . . 2701 2704 + TaqI TCGA 2701 2703 . . 2719 2722 + MaeI CTAG 2719 2721 . . 2722 2726 + MaeIII GTNAC 2721 2726 . . 2725 2729 + Tsp4CI* ACNGT 2727 2726 . . 2728 2732 + MaeIII GTNAC 2727 2732 . . 2740 2744 + TfiI GAWTC 2740 2743 . . 2740 2744 + HinfI GANTC 2740 2743 . . 2755 2759 + Hpy188I TCNGA 2757 2756 . . 2768 2774 + AarI CACCTGC 2778 2782 . . 2769 2772 + SetI ASST 2772 2768 . . 2769 2774 + BspMI ACCTGC 2778 2782 . . 2767 2771 - HphI GGTGA 2759 2758 . . 2782 2785 + TaqI TCGA 2782 2784 . . 2783 2787 + Hpy99I CGWCG 2787 2782 . . 2785 2789 + BceAI ACGGC 2801 2803 . . 2786 2791 + CfrI YGGCCR 2786 2790 . . 2787 2790 + CviJI RGCY 2788 2788 . . 2787 2790 + HaeIII GGCC 2788 2788 . . 2789 2794 + DsaI* CCRYGG 2789 2793 . . 2789 2794 + SecI* CCNNGG 2789 2793 . . 2791 2795 + BceAI ACGGC 2807 2809 . . 2793 2796 + CviJI RGCY 2794 2794 . . 2797 2801 + Tsp4CI* ACNGT 2799 2798 . . 2812 2816 + MaeIII GTNAC 2811 2816 . . 2815 2819 + Tsp4CI* ACNGT 2817 2816 . . 2828 2831 + FatI CATG 2827 2831 . . 2828 2831 + NlaIII CATG 2831 2827 . . 2828 2831 + CviAII CATG 2828 2830 . . 2832 2836 - BsmAI GTCTC 2826 2830 . . 2832 2837 - Eco31I GGTCTC 2826 2830 . . 2849 2853 + TspDTI ATGAA 2864 2862 . . 2853 2857 + Tsp4CI* ACNGT 2855 2854 . . 2863 2866 + SetI ASST 2866 2862 . . 2863 2866 + CviJI RGCY 2864 2864 . . 2863 2866 + AluI AGCT 2864 2864 . . 2872 2876 + TspDTI ATGAA 2887 2885 . . 2876 2880 + Tsp4CI* ACNGT 2878 2877 . . 2877 2883 + TspRI CASTGNN 2883 2874 . . 2886 2892 + TspRI CASTGNN 2892 2883 . . 2885 2889 - BsrI ACTGG 2885 2883 . . 2890 2894 - BsmAI GTCTC 2884 2888 . . 2900 2910 + BsiYI* CCNNNNNNNGG 2906 2903 . . 2905 2909 + BsrI ACTGG 2910 2908 . . 2905 2910 + BfiI ACTGGG 2915 2914 . . 2910 2913 + CviJI RGCY 2911 2911 . . 2911 2915 + BlsI GCNGC 2913 2912 . . 2911 2915 + BisI GCNGC 2912 2913 . . 2911 2915 + TseI GCWGC 2911 2914 . . 2915 2919 + DdeI CTNAG 2915 2918 . . 2911 2915 - BbvI GCAGC 2898 2902 . . 2912 2917 - EcoP15I CAGCAG 2884 2886 . . 2915 2919 - BspCNI CTCAG 2907 2905 . . 2915 2919 - BseMII CTCAG 2906 2904 . . 2917 2921 - BsmAI GTCTC 2911 2915 . . 2927 2932 + EcoP15I CAGCAG 2957 2959 . . 2932 2936 + Tsp45I GTSAC 2931 2936 . . 2932 2936 + MaeIII GTNAC 2931 2936 . . 2935 2938 + SetI ASST 2938 2934 . . 2936 2939 + MnlI CCTC 2946 2945 . . 2937 2942 + Ksp632I* CTCTTC 2943 2946 . . 2933 2937 - HphI GGTGA 2925 2924 . . 2938 2942 - MboII GAAGA 2930 2929 . . 2947 2952 + Hpy178III* TCNNGA 2948 2950 . . 2951 2955 + TfiI GAWTC 2951 2954 . . 2951 2955 + HinfI GANTC 2951 2954 . . 2976 2979 + SetI ASST 2979 2975 . . 2976 2979 + CviJI RGCY 2977 2977 . . 2976 2979 + AluI AGCT 2977 2977 . . 2981 2984 + AciI CCGC 2981 2983 . . 2982 2985 + FnuDII* CGCG 2983 2983 . . 2984 2989 + McrI* CGRYCG 2987 2985 . . 2985 2990 + TaqII GACCGA 3001 2999 . . 2999 3003 + Tsp45I GTSAC 2998 3003 . . 2999 3003 + MaeIII GTNAC 2998 3003 . . 3003 3008 + EcoP15I CAGCAG 3033 3035 . . 3022 3027 + BciVI GTATCC 3033 3032 . . 3025 3029 + Hpy188I TCNGA 3027 3026 . . 3031 3035 + BsrI ACTGG 3036 3034 . . 3042 3045 + FatI CATG 3041 3045 . . 3042 3045 + NlaIII CATG 3045 3041 . . 3042 3045 + CviAII CATG 3042 3044 . . 3045 3049 + PleI GAGTC 3053 3054 . . 3045 3049 + MlyI GAGTC 3054 3054 . . 3045 3049 + HinfI GANTC 3045 3048 . . 3059 3063 + BssKI CCNGG 3058 3063 . . 3059 3062 + HpaII CCGG 3059 3061 . . 3059 3063 + ScrFI CCNGG 3060 3061 . . 3059 3063 + CauII* CCSGG 3060 3061 . . 3066 3071 + SduI GDGCHC 3070 3066 . . 3066 3071 + HgiAI* GWGCWC 3070 3066 . . 3083 3086 + CviJI RGCY 3084 3084 . . 3085 3088 + MnlI CCTC 3095 3094 . . 3078 3089 - BcgI CGANNNNNNTGC 3101 3099 3067 3065 3108 3111 + FatI CATG 3107 3111 . . 3108 3111 + NlaIII CATG 3111 3107 . . 3108 3111 + CviAII CATG 3108 3110 . . 3115 3120 + XhoII RGATCY 3115 3119 . . 3115 3119 + BinI* GGATC 3123 3124 . . 3116 3119 + DpnI GATC 3117 3117 . . 3116 3119 + BstKTI GATC 3118 3116 . . 3116 3119 + MboI GATC 3115 3119 . . 3119 3122 + MaeI CTAG 3119 3121 . . 3121 3126 + TatI WGTACW 3121 3125 . . 3122 3125 + Csp6I GTAC 3122 3124 . . 3122 3125 + RsaI GTAC 3123 3123 . . 3126 3129 + SetI ASST 3129 3125 . . 3126 3129 + CviJI RGCY 3127 3127 . . 3126 3129 + AluI AGCT 3127 3127 . . 3137 3142 + ApoI RAATTY 3137 3141 . . 3138 3141 + TspEI AATT 3137 3141 . . 3145 3148 + TaiI ACGT 3148 3144 . . 3145 3148 + SetI ASST 3148 3144 . . 3145 3148 + MaeII ACGT 3145 3147 . . 3151 3161 + MwoI GCNNNNNNNGC 3157 3154 . . 3151 3156 + Cac8I GCNNGC 3153 3153 . . 3155 3160 + BtsI GCAGTG 3162 3160 . . 3156 3162 + TspRI CASTGNN 3162 3153 . . 3161 3175 + XcmI CCANNNNNNNNNTGG 3168 3167 . . 3166 3169 + SetI ASST 3169 3165 . . 3166 3169 + CviJI RGCY 3167 3167 . . 3166 3169 + AluI AGCT 3167 3167 . . 3171 3175 + BsrI ACTGG 3176 3174 . . 3173 3178 + CfrI YGGCCR 3173 3177 . . 3174 3177 + CviJI RGCY 3175 3175 . . 3174 3177 + HaeIII GGCC 3175 3175 . . 3175 3180 + Cfr10I RCCGGY 3175 3179 . . 3176 3179 + HpaII CCGG 3176 3178 . . 3187 3190 + MseI TTAA 3187 3189 . . 3194 3198 - MboII GAAGA 3186 3185 . . 3193 3198 - BbvII* GAAGAC 3186 3190 . . 3196 3200 - TspDTI ATGAA 3186 3184 . . 3198 3203 - BsrDI GCAATG 3197 3195 . . 3202 3206 - HgaI GACGC 3191 3196 . . 3213 3218 + Cac8I GCNNGC 3215 3215 . . 3219 3222 + TspEI AATT 3218 3222 . . 3225 3228 + MseI TTAA 3225 3227 . . #--------------------------------------- # # # AarI: # [CACCTGC] # 450 456 2322 # # Acc65I: # [GGTACC] # 135 135 135 135 135 135 # 1030 1388 # # AccI: # [GTMKAC] # 70 177 553 599 1829 # # AciI: # [CCGC] # 247 546 649 1786 # # AcyI: # [GRCGYC] # 790 2438 # # AflIII: # [ACRYGT] # 171 455 2602 # # AgeI: # [ACCGGT] # 42 42 42 93 93 135 # 135 135 270 850 1391 # # AjuI: # [GAANNNNNNNTTGG] # 32 1063 2133 # # AlfI: # [GCANNNNNNTGC] # 33 3195 # # AluI: # [AGCT] # 40 61 113 150 924 952 # 988 # # AlwNI: # [CAGNNNCTG] # 619 2609 # # ApaLI: # [GTGCAC] # 527 2701 # # ApoI: # [RAATTY] # 91 123 893 2121 # # AsuI*: # [GGNCC] # 135 135 135 135 270 848 # 1570 # # AsuII: # [TTCGAA] # 163 355 2710 # # AvaII: # [GGWCC] # 135 135 135 135 270 848 # 1570 # # BaeI: # [ACNNNNGTAYC] # 33 710 2485 # # BarI: # [GAAGNNNNNNTAC] # 32 32 32 168 646 913 # 1405 # # BbvI: # [GCAGC] # 181 330 2717 # # BbvII*: # [GAAGAC] # 42 1243 1943 # # BccI: # [CCATC] # 30 66 72 126 186 270 # 270 333 495 553 827 # # Bce83I*: # [CTTGAG] # 275 2953 # # BceAI: # [ACGGC] # 6 134 235 421 658 1774 # # BcgI: # [CGANNNNNNTGC] # 10 10 24 34 127 498 # 2525 # # BciVI: # [GTATCC] # 195 3033 # # BdaI: # [TGANNNNNNTCA] # 0 0 0 0 0 0 # 0 0 34 34 34 34 # 79 101 371 757 1784 # # BetI*: # [WCCGGW] # 42 42 42 42 93 93 # 135 135 135 228 850 1391 # # BfiI: # [ACTGGG] # 313 321 2594 # # BglI: # [GCCNNNNNGGC] # 33 46 3149 # # BinI*: # [GGATC] # 105 793 2330 # # BisI: # [GCNGC] # 170 316 476 2266 # # BlsI: # [GCNGC] # 171 315 476 2266 # # BmgT120I: # [GGNCC] # 135 135 135 135 270 849 # 1569 # # BmtI: # [GCTAGC] # 956 2272 # # BsaAI: # [YACGTR] # 769 2459 # # BsaXI: # [ACNNNNNCTCC] # 30 600 2598 # # BseBI: # [CCWGG] # 681 938 1609 # # BseGI: # [GGATG] # 119 135 135 135 203 225 # 225 234 272 274 405 866 # # BseMII: # [CTCAG] # 80 135 135 135 135 135 # 135 135 135 213 322 747 # 786 # # BseRI: # [GAGGAG] # 390 840 1998 # # BseSI: # [GKGCMC] # 157 531 761 1779 # # BsiYI*: # [CCNNNNNNNGG] # 5 5 5 5 5 5 # 5 91 130 130 130 130 # 130 130 135 144 165 192 # 322 445 919 # # BslFI: # [GGGAC] # 21 630 690 1887 # # BsmAI: # [GTCTC] # 27 58 236 317 350 601 # 1639 # # BsmI: # [GAATGC] # 171 3057 # # BspCNI: # [CTCAG] # 79 135 135 135 135 135 # 135 135 137 211 321 747 # 788 # # BspLU11I*: # [ACATGT] # 626 2602 # # BspMI: # [ACCTGC] # 450 456 2322 # # BsrDI: # [GCAATG] # 31 509 1205 1483 # # BsrI: # [ACTGG] # 25 42 43 51 52 107 # 126 129 132 140 184 185 # 228 237 612 935 # # BssKI: # [CCNGG] # 170 679 770 1609 # # BssNAI: # [GTATAC] # 775 2453 # # BstEII: # [GGTNACC] # 270 270 528 876 1284 # # BstKTI: # [GATC] # 110 801 2317 # # BtrI: # [CACGTC] # 551 1279 1398 # # BtsI: # [GCAGTG] # 15 66 526 2621 # # Cac8I: # [GCNNGC] # 12 13 52 62 84 266 # 540 2199 # # CauII*: # [CCSGG] # 168 3060 # # Cfr10I: # [RCCGGY] # 42 42 42 53 93 93 # 135 135 135 270 850 1338 # # CfrI: # [YGGCCR] # 55 369 387 2417 # # Csp6I: # [GTAC] # 28 41 42 42 42 42 # 42 42 66 93 93 93 # 93 93 106 135 135 351 # 368 543 738 # # CspCI: # [CAANNNNNGTGG] # 35 683 2510 # # CviAII: # [CATG] # 43 66 120 135 135 135 # 135 135 135 135 153 214 # 218 298 584 587 # # CviJI: # [RGCY] # 6 8 9 24 25 26 # 40 40 43 47 53 56 # 66 70 83 100 107 109 # 131 168 201 205 248 401 # 962 # # CviRI*: # [TGCA] # 10 10 18 38 42 105 # 111 149 151 2594 # # DdeI: # [CTNAG] # 66 103 135 135 135 135 # 135 135 135 158 190 312 # 313 435 706 # # DpnI: # [GATC] # 111 800 2317 # # DraIII: # [CACNNNGTG] # 766 2462 # # DsaI*: # [CCRYGG] # 135 135 135 135 135 135 # 135 261 375 439 583 625 # # Eco31I: # [GGTCTC] # 402 2826 # # Eco57I: # [CTGAAG] # 102 673 1219 1234 # # Eco57MI: # [CTGRAG] # 102 289 384 591 628 1234 # # EcoP15I: # [CAGCAG] # 73 76 122 195 2762 # # EcoRII: # [CCWGG] # 679 940 1609 # # FatI: # [CATG] # 43 66 121 135 135 135 # 135 135 135 135 153 214 # 218 298 583 587 # # FnuDII*: # [CGCG] # 245 1027 1956 # # FokI: # [GGATG] # 126 135 135 135 203 225 # 225 234 254 272 405 879 # # GsuI: # [CTGGAG] # 384 628 2216 # # HaeIII: # [GGCC] # 24 34 40 53 131 369 # 387 2190 # # HgaI: # [GACGC] # 37 482 764 1945 # # HgiAI*: # [GWGCWC] # 158 531 2539 # # HgiCI*: # [GGYRCC] # 135 135 135 135 135 135 # 622 766 1030 # # Hin4I: # [GAYNNNNNVTC] # 14 14 18 598 2584 # # Hin4II*: # [CCTTC] # 76 88 648 674 1742 # # HindII: # [GTYRAC] # 135 135 135 135 135 135 # 488 554 1376 # # HinfI: # [GANTC] # 18 42 94 108 111 147 # 148 150 183 183 198 211 # 1635 # # HpaII: # [CCGG] # 42 42 42 42 52 93 # 93 117 135 135 135 228 # 851 1221 # # HphI: # [GGTGA] # 30 30 99 105 105 105 # 111 135 135 135 165 166 # 182 303 540 882 # # Hpy166II: # [GTNNAC] # 25 39 69 70 90 135 # 135 135 135 135 135 177 # 289 529 532 598 # # Hpy178III*: # [TCNNGA] # 68 224 280 1023 1633 # # Hpy188I: # [TCNGA] # 54 54 165 201 220 233 # 270 369 405 540 717 # # Hpy99I: # [CGWCG] # 441 555 2232 # # KpnI: # [GGTACC] # 135 135 135 135 135 135 # 1034 1384 # # Ksp632I*: # [CTCTTC] # 9 9 150 285 720 2055 # # MaeI: # [CTAG] # 18 59 109 129 135 135 # 135 135 148 204 207 208 # 270 400 406 530 # # MaeII: # [ACGT] # 53 83 93 199 404 510 # 687 1199 # # MaeIII: # [GTNAC] # 6 6 15 15 66 66 # 67 84 84 87 120 126 # 126 126 129 135 144 144 # 204 225 230 405 618 # # MboI: # [GATC] # 113 798 2317 # # MboII: # [GAAGA] # 3 3 3 3 3 3 # 6 6 9 12 12 15 # 15 30 30 30 42 51 # 69 81 256 276 307 360 # 1603 # # McrI*: # [CGRYCG] # 241 2987 # # MfeI: # [CAATTG] # 5 3223 # # MlyI: # [GAGTC] # 174 3054 # # MmeI: # [TCCRAC] # 135 135 135 135 135 135 # 135 198 252 769 1064 # # MnlI: # [CCTC] # 3 3 3 3 9 10 # 20 21 27 39 42 42 # 60 60 60 63 63 63 # 67 72 72 72 72 72 # 72 85 132 133 149 192 # 201 252 457 537 # # MseI: # [TTAA] # 3 38 56 539 854 1738 # # MslI: # [CAYNNNNRTG] # 135 135 540 877 1541 # # MwoI: # [GCNNNNNNNGC] # 9 24 46 71 99 540 # 2439 # # NcoI: # [CCATGG] # 135 135 135 135 135 135 # 135 261 583 1439 # # NdeI: # [CATATG] # 632 2596 # # NheI: # [GCTAGC] # 952 2276 # # NlaIII: # [CATG] # 43 66 117 135 135 135 # 135 135 135 135 153 214 # 218 298 587 587 # # NlaIV: # [GGNNCC] # 72 135 135 135 135 135 # 135 622 764 960 # # NspBII*: # [CMGCKG] # 651 2577 # # NspI: # [RCATGY] # 630 2598 # # PflMI: # [CCANNNNNTGG] # 270 589 936 1433 # # PleI: # [GAGTC] # 175 3053 # # PmaCI: # [CACGTG] # 769 2459 # # PshAI: # [GACNNNNGTC] # 248 405 773 1802 # # PsiI: # [TTATAA] # 248 2980 # # PsrI: # [GAACNNNNNNTAC] # 32 1321 1875 # # RsaI: # [GTAC] # 28 41 42 42 42 42 # 42 42 66 93 93 93 # 93 93 105 135 135 351 # 369 543 738 # # SalI: # [GTCGAC] # 552 2676 # # SauI*: # [CCTNAGG] # 66 3162 # # ScaI: # [AGTACT] # 369 2859 # # ScrFI: # [CCNGG] # 168 681 770 1609 # # SduI: # [GDGCHC] # 157 158 531 603 1779 # # SecI*: # [CCNNGG] # 21 84 97 135 135 135 # 135 135 135 135 164 270 # 439 583 625 # # SetI: # [ASST] # 9 10 16 16 19 19 # 21 21 34 41 43 46 # 48 48 59 60 72 83 # 92 93 94 99 106 110 # 116 119 119 135 135 145 # 150 151 181 194 213 311 # # SfaNI: # [GCATC] # 307 2921 # # SmlI: # [CTYRAG] # 290 2938 # # SpeI: # [ACTAGT] # 135 135 135 270 1081 1472 # # SspI: # [AATATT] # 252 2976 # # StyI: # [CCWWGG] # 21 135 135 135 135 135 # 135 135 261 583 709 709 # # TaiI: # [ACGT] # 53 80 93 199 407 510 # 687 1199 # # TaqI: # [TCGA] # 35 75 81 163 280 446 # 2148 # # TaqII: # [CACCCA] # 227 319 2682 # # TatI: # [WGTACW] # 107 367 420 738 1596 # # TauI: # [GCSGC] # 790 2438 # # TfiI: # [GAWTC] # 18 42 108 111 147 148 # 150 183 198 211 277 1635 # # TseI: # [GCWGC] # 169 317 2742 # # TsoI: # [TARCCA] # 887 2341 # # Tsp45I: # [GTSAC] # 15 67 81 135 230 270 # 270 315 330 492 1023 # # Tsp4CI*: # [ACNGT] # 6 16 16 16 16 18 # 20 20 20 20 20 20 # 20 20 23 38 66 72 # 99 99 99 99 105 115 # 115 115 135 147 162 178 # 350 474 489 # # TspDTI: # [ATGAA] # 2 4 23 42 51 127 # 128 194 299 570 675 1113 # # TspEI: # [AATT] # 5 10 11 48 54 70 # 77 81 124 135 207 229 # 230 329 564 1054 # # TspGWI: # [ACGGA] # 1107 2121 # # TspRI: # [CASTGNN] # 9 18 22 24 54 66 # 117 135 135 135 192 240 # 270 363 591 857 # # TstI: # [CACNNNNNNTCC] # 32 1214 1982 # # VspI: # [ATTAAT] # 56 3172 # # XcmI: # [CCANNNNNNNNNTGG] # 60 106 3062 # # XhoII: # [RGATCY] # 113 3115 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 3228 # Reported_sequences: 1 # Reported_hitcount: 912 #---------------------------------------