Restriction Fragments for CTF8/YHR191C



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The currently selected gene/sequence is :

CTF8/YHR191C on chromosome VIII from coordinates 486631 to 486230.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.26720.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 5 8 + MaeI CTAG 5 7 . . 19 23 - SfaNI GCATC 9 13 . . 23 26 + MaeI CTAG 23 25 . . 39 44 + Hpy166II GTNNAC 41 41 . . 39 44 + HindII GTYRAC 41 41 . . 42 50 + Tth111I GACNNNGTC 45 46 . . 45 49 + Tsp4CI* ACNGT 47 46 . . 49 54 + Hpy178III* TCNNGA 50 52 . . 65 70 + DsaI* CCRYGG 65 69 . . 65 70 + SecI* CCNNGG 65 69 . . 67 71 + Tsp4CI* ACNGT 69 68 . . 69 73 + HphI GGTGA 81 80 . . 79 82 + AciI CCGC 79 81 . . 86 90 + FokI GGATG 99 103 . . 86 90 + BseGI GGATG 92 90 . . 93 97 - SfaNI GCATC 83 87 . . 97 102 + Eco57MI CTGRAG 118 116 . . 97 102 + GsuI CTGGAG 118 116 . . 102 106 + TfiI GAWTC 102 105 . . 102 106 + HinfI GANTC 102 105 . . 108 111 + SetI ASST 111 107 . . 109 113 + HphI GGTGA 121 120 . . 110 115 + Hpy166II GTNNAC 112 112 . . 115 118 + MaeI CTAG 115 117 . . 124 130 + SauI* CCTNAGG 125 128 . . 125 129 + DdeI CTNAG 125 128 . . 130 140 + Hin4I GAYNNNNNVTC 121 116 153 148 130 140 + Eam1105I GACNNNNNGTC 135 134 . . 136 146 + MwoI GCNNNNNNNGC 142 139 . . 137 141 + Hpy99I CGWCG 141 136 . . 130 140 - Hin4I GAYNNNNNVTC 153 148 121 116 136 140 - HgaI GACGC 125 130 . . 141 146 + Cac8I GCNNGC 143 143 . . 147 152 + BseRI GAGGAG 162 160 . . 147 150 - MnlI CCTC 140 139 . . 150 160 + BplI GAGNNNNNCTC 141 136 173 168 154 158 + TfiI GAWTC 154 157 . . 154 158 + HinfI GANTC 154 157 . . 150 160 - BplI GAGNNNNNCTC 173 168 141 136 160 170 + BsiYI* CCNNNNNNNGG 166 163 . . 166 169 - MnlI CCTC 159 158 . . 172 175 + SetI ASST 175 171 . . 170 174 - Hin4II* CCTTC 164 163 . . 177 183 + TspRI CASTGNN 183 174 . . 166 177 - PpiI GAACNNNNNCTC 189 184 157 152 179 184 + Hpy166II GTNNAC 181 181 . . 188 192 + BceAI ACGGC 204 206 . . 199 202 + MseI TTAA 199 201 . . 216 220 + MaeIII GTNAC 215 220 . . 227 231 + TspGWI ACGGA 242 240 . . 236 240 + BmgT120I GGNCC 237 238 . . 236 240 + AsuI* GGNCC 236 239 . . 234 237 - MnlI CCTC 227 226 . . 237 240 + CviJI RGCY 238 238 . . 237 240 + HaeIII GGCC 238 238 . . 239 250 - CspCI CAANNNNNGTGG 263 261 228 226 266 271 + Hpy166II GTNNAC 268 268 . . 270 274 + BsrI ACTGG 275 273 . . 270 275 + BfiI ACTGGG 280 279 . . 278 281 + SetI ASST 281 277 . . 279 283 + HphI GGTGA 291 290 . . 280 284 + Tsp45I GTSAC 279 284 . . 280 284 + MaeIII GTNAC 279 284 . . 276 280 - Hin4II* CCTTC 270 269 . . 287 290 + SetI ASST 290 286 . . 287 290 + CviJI RGCY 288 288 . . 287 290 + AluI AGCT 288 288 . . 289 292 + MaeI CTAG 289 291 . . 293 296 + TaiI ACGT 296 292 . . 293 296 + SetI ASST 296 292 . . 293 296 + MaeII ACGT 293 295 . . 295 298 + Csp6I GTAC 295 297 . . 295 298 + RsaI GTAC 296 296 . . 300 304 - BccI CCATC 294 295 . . 310 315 + EcoT22I ATGCAT 314 310 . . 311 314 + CviRI* TGCA 312 312 . . 319 322 + TspEI AATT 318 322 . . 321 326 + AsuII TTCGAA 322 324 . . 322 325 + TaqI TCGA 322 324 . . 335 338 + SetI ASST 338 334 . . 338 341 + TaqI TCGA 338 340 . . 341 344 + TspEI AATT 340 344 . . 355 359 + TspDTI ATGAA 370 368 . . 364 374 + FalI AAGNNNNNCTT 355 350 387 382 365 368 + SetI ASST 368 364 . . 374 377 + MseI TTAA 374 376 . . 364 374 - FalI AAGNNNNNCTT 387 382 355 350 382 387 + StuI AGGCCT 384 384 . . 383 386 + CviJI RGCY 384 384 . . 383 386 + HaeIII GGCC 384 384 . . 385 388 + MnlI CCTC 395 394 . . 390 393 + SetI ASST 393 389 . . #--------------------------------------- # # # AciI: # [CCGC] # 79 323 # # AluI: # [AGCT] # 114 288 # # AsuI*: # [GGNCC] # 166 236 # # AsuII: # [TTCGAA] # 80 322 # # BccI: # [CCATC] # 108 294 # # BceAI: # [ACGGC] # 198 204 # # BfiI: # [ACTGGG] # 122 280 # # BmgT120I: # [GGNCC] # 165 237 # # BplI: # [GAGNNNNNCTC] # 0 0 32 141 229 # # BseGI: # [GGATG] # 92 310 # # BseRI: # [GAGGAG] # 162 240 # # BsiYI*: # [CCNNNNNNNGG] # 166 236 # # BsrI: # [ACTGG] # 127 275 # # Cac8I: # [GCNNGC] # 143 259 # # Csp6I: # [GTAC] # 107 295 # # CspCI: # [CAANNNNNGTGG] # 35 139 228 # # CviJI: # [RGCY] # 18 50 96 238 # # CviRI*: # [TGCA] # 90 312 # # DdeI: # [CTNAG] # 125 277 # # DsaI*: # [CCRYGG] # 65 337 # # Eam1105I: # [GACNNNNNGTC] # 135 267 # # Eco57MI: # [CTGRAG] # 118 284 # # EcoT22I: # [ATGCAT] # 88 314 # # FalI: # [AAGNNNNNCTT] # 0 0 15 32 355 # # FokI: # [GGATG] # 99 303 # # GsuI: # [CTGGAG] # 118 284 # # HaeIII: # [GGCC] # 18 146 238 # # HgaI: # [GACGC] # 125 277 # # Hin4I: # [GAYNNNNNVTC] # 0 0 32 121 249 # # Hin4II*: # [CCTTC] # 106 132 164 # # HindII: # [GTYRAC] # 41 361 # # HinfI: # [GANTC] # 52 102 248 # # HphI: # [GGTGA] # 40 81 111 170 # # Hpy166II: # [GTNNAC] # 41 69 71 87 134 # # Hpy178III*: # [TCNNGA] # 50 352 # # Hpy99I: # [CGWCG] # 141 261 # # MaeI: # [CTAG] # 5 18 92 113 174 # # MaeII: # [ACGT] # 109 293 # # MaeIII: # [GTNAC] # 64 123 215 # # MnlI: # [CCTC] # 7 19 68 140 168 # # MseI: # [TTAA] # 28 175 199 # # MwoI: # [GCNNNNNNNGC] # 142 260 # # PpiI: # [GAACNNNNNCTC] # 32 157 213 # # RsaI: # [GTAC] # 106 296 # # SauI*: # [CCTNAGG] # 125 277 # # SecI*: # [CCNNGG] # 65 337 # # SetI: # [ASST] # 6 9 9 25 30 42 # 64 106 111 # # SfaNI: # [GCATC] # 9 74 319 # # StuI: # [AGGCCT] # 18 384 # # TaiI: # [ACGT] # 106 296 # # TaqI: # [TCGA] # 16 64 322 # # TfiI: # [GAWTC] # 52 102 248 # # Tsp45I: # [GTSAC] # 123 279 # # Tsp4CI*: # [ACNGT] # 22 47 333 # # TspDTI: # [ATGAA] # 32 370 # # TspEI: # [AATT] # 22 62 318 # # TspGWI: # [ACGGA] # 160 242 # # TspRI: # [CASTGNN] # 183 219 # # Tth111I: # [GACNNNGTC] # 45 357 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 402 # Reported_sequences: 1 # Reported_hitcount: 92 #---------------------------------------