Restriction Fragments for KOG1/YHR186C



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The currently selected gene/sequence is :

KOG1/YHR186C on chromosome VIII from coordinates 480672 to 475999.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.9369.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 4 7 + HpaII CCGG 4 6 . . 16 20 + BmgT120I GGNCC 17 18 . . 16 20 + AvaII GGWCC 16 19 . . 16 20 + AsuI* GGNCC 16 19 . . 18 21 + SetI ASST 21 17 . . 19 22 + MnlI CCTC 29 28 . . 19 25 + Bpu10I CCTNAGC 20 23 . . 19 25 + BbvCI CCTCAGC 20 23 . . 20 24 + DdeI CTNAG 20 23 . . 20 24 + BspCNI CTCAG 33 31 . . 20 24 + BseMII CTCAG 34 32 . . 23 26 + CviJI RGCY 24 24 . . 24 34 + MwoI GCNNNNNNNGC 30 27 . . 28 31 + MseI TTAA 28 30 . . 32 35 + CviJI RGCY 33 33 . . 43 47 + Tsp4CI* ACNGT 45 44 . . 45 49 + HphI GGTGA 57 56 . . 55 58 + FatI CATG 54 58 . . 55 58 + NlaIII CATG 58 54 . . 55 58 + CviAII CATG 55 57 . . 51 55 - BsmAI GTCTC 45 49 . . 64 68 + MboII GAAGA 76 75 . . 64 69 - Ksp632I* CTCTTC 59 62 . . 64 70 - SapI GCTCTTC 59 62 . . 80 84 + Tsp45I GTSAC 79 84 . . 80 84 + MaeIII GTNAC 79 84 . . 101 106 + Hpy178III* TCNNGA 102 104 . . 101 106 + NruI TCGCGA 103 103 . . 102 105 + FnuDII* CGCG 103 103 . . 105 114 + XmnI GAANNNNTTC 109 109 . . 112 116 - TspDTI ATGAA 102 100 . . 156 160 + Hpy188I TCNGA 158 157 . . 159 163 + TfiI GAWTC 159 162 . . 159 163 + HinfI GANTC 159 162 . . 162 167 + Hpy178III* TCNNGA 163 165 . . 164 169 + Eco57MI CTGRAG 185 183 . . 164 169 + Eco57I CTGAAG 185 183 . . 166 170 + MboII GAAGA 178 177 . . 166 171 - Ksp632I* CTCTTC 161 164 . . 169 172 - MnlI CCTC 162 161 . . 184 193 + BsaBI GATNNNNATC 188 188 . . 203 206 + CviJI RGCY 204 204 . . 219 223 + MboII GAAGA 231 230 . . 226 230 + TspDTI ATGAA 241 239 . . 232 235 + DpnI GATC 233 233 . . 232 235 + BstKTI GATC 234 232 . . 232 235 + MboI GATC 231 235 . . 234 237 + TaqI TCGA 234 236 . . 240 244 + MboII GAAGA 252 251 . . 240 245 + BbvII* GAAGAC 247 251 . . 244 248 + Tsp4CI* ACNGT 246 245 . . 253 257 + TauI GCSGC 256 253 . . 253 257 + BlsI GCNGC 255 254 . . 253 257 + BisI GCNGC 254 255 . . 255 258 + CviJI RGCY 256 256 . . 255 258 + HaeIII GGCC 256 256 . . 255 259 + BmgT120I GGNCC 256 257 . . 255 259 + AsuI* GGNCC 255 258 . . 253 256 - AciI CCGC 253 255 . . 273 278 + ApoI RAATTY 273 277 . . 274 277 + TspEI AATT 273 277 . . 279 282 + SetI ASST 282 278 . . 284 287 + TaqI TCGA 284 286 . . 286 289 + DpnI GATC 287 287 . . 286 289 + BstKTI GATC 288 286 . . 286 289 + MboI GATC 285 289 . . 286 290 - BinI* GGATC 280 281 . . 301 305 + TspDTI ATGAA 316 314 . . 303 307 + MboII GAAGA 315 314 . . 303 308 + BbvII* GAAGAC 310 314 . . 310 319 + MslI CAYNNNNRTG 314 314 . . 310 314 - FokI GGATG 296 300 . . 310 314 - BseGI GGATG 309 307 . . 332 335 + FatI CATG 331 335 . . 332 335 + NlaIII CATG 335 331 . . 332 335 + CviAII CATG 332 334 . . 337 342 + Hpy166II GTNNAC 339 339 . . 337 342 + AccI GTMKAC 338 340 . . 348 353 + ApoI RAATTY 348 352 . . 349 352 + TspEI AATT 348 352 . . 353 359 + PfoI TCCNGGA 353 358 . . 354 358 + BssKI CCNGG 353 358 . . 354 358 + BseBI CCWGG 355 356 . . 354 358 + ScrFI CCNGG 355 356 . . 354 358 + EcoRII CCWGG 353 358 . . 358 362 + HinfI GANTC 358 361 . . 361 364 + TaqI TCGA 361 363 . . 358 362 - PleI GAGTC 352 353 . . 358 362 - MlyI GAGTC 352 352 . . 363 367 + MboII GAAGA 375 374 . . 381 384 + TspEI AATT 380 384 . . 402 406 + Tsp4CI* ACNGT 404 403 . . 405 408 + Csp6I GTAC 405 407 . . 405 408 + RsaI GTAC 406 406 . . 441 446 + HindIII AAGCTT 441 445 . . 442 445 + SetI ASST 445 441 . . 442 445 + CviJI RGCY 443 443 . . 442 445 + AluI AGCT 443 443 . . 447 452 + XhoII RGATCY 447 451 . . 447 452 + NlaIV GGNNCC 449 449 . . 447 452 + BamHI GGATCC 447 451 . . 447 451 + BinI* GGATC 455 456 . . 448 451 + DpnI GATC 449 449 . . 448 451 + BstKTI GATC 450 448 . . 448 451 + MboI GATC 447 451 . . 448 452 - BinI* GGATC 442 443 . . 462 466 + FokI GGATG 475 479 . . 462 466 + BseGI GGATG 468 466 . . 460 463 - MnlI CCTC 453 452 . . 471 475 + Tsp4CI* ACNGT 473 472 . . 478 481 + CviRI* TGCA 479 479 . . 487 491 + DdeI CTNAG 487 490 . . 489 499 + FalI AAGNNNNNCTT 480 475 512 507 491 495 + MboII GAAGA 503 502 . . 491 500 + XmnI GAANNNNTTC 495 495 . . 489 499 - FalI AAGNNNNNCTT 512 507 480 475 500 504 + DdeI CTNAG 500 503 . . 505 509 + MboII GAAGA 517 516 . . 505 517 + BarI GAAGNNNNNNTAC 497 492 529 524 512 517 - BciVI GTATCC 506 505 . . 530 534 + TspGWI ACGGA 545 543 . . 535 538 + FatI CATG 534 538 . . 535 538 + NlaIII CATG 538 534 . . 535 538 + CviAII CATG 535 537 . . 532 543 - TstI CACNNNNNNTCC 556 551 524 519 544 550 + Bpu10I CCTNAGC 545 548 . . 545 549 + DdeI CTNAG 545 548 . . 548 551 + CviJI RGCY 549 549 . . 561 564 + SetI ASST 564 560 . . 562 566 + HphI GGTGA 574 573 . . 566 571 + ApoI RAATTY 566 570 . . 567 570 + TspEI AATT 566 570 . . 585 588 + SetI ASST 588 584 . . 584 587 - MnlI CCTC 577 576 . . 603 608 + BetI* WCCGGW 603 607 . . 604 607 + HpaII CCGG 604 606 . . 620 623 + SetI ASST 623 619 . . 620 625 + BspMI ACCTGC 629 633 . . 623 626 + CviRI* TGCA 624 624 . . 631 636 - TsoI TARCCA 621 619 . . 636 639 + SetI ASST 639 635 . . 640 643 + Hin6I GCGC 640 642 . . 640 643 + GlaI GCGC 641 641 . . 640 643 + HhaI GCGC 642 640 . . 644 647 + FatI CATG 643 647 . . 644 647 + NlaIII CATG 647 643 . . 644 647 + CviAII CATG 644 646 . . 664 667 + CviRI* TGCA 665 665 . . 668 672 + Tsp4CI* ACNGT 670 669 . . 669 675 + TspRI CASTGNN 675 666 . . 684 689 + VspI ATTAAT 685 687 . . 685 688 + MseI TTAA 685 687 . . 696 700 + Hpy188I TCNGA 698 697 . . 707 710 + CviRI* TGCA 708 708 . . 714 717 - MnlI CCTC 707 706 . . 719 722 + MseI TTAA 719 721 . . 728 732 + TspDTI ATGAA 743 741 . . 730 734 + MboII GAAGA 742 741 . . 730 735 - Ksp632I* CTCTTC 725 728 . . 733 736 - MnlI CCTC 726 725 . . 741 746 + Hpy178III* TCNNGA 742 744 . . 741 746 + BspHI TCATGA 741 745 . . 742 745 + FatI CATG 741 745 . . 742 745 + NlaIII CATG 745 741 . . 742 745 + CviAII CATG 742 744 . . 751 755 + TauI GCSGC 754 751 . . 751 755 + BlsI GCNGC 753 752 . . 751 755 + BisI GCNGC 752 753 . . 752 755 + AciI CCGC 752 754 . . 753 756 + FnuDII* CGCG 754 754 . . 754 757 + Hin6I GCGC 754 756 . . 754 757 + GlaI GCGC 755 755 . . 754 757 + HhaI GCGC 756 754 . . 757 761 + BccI CCATC 765 766 . . 760 764 - HphI GGTGA 752 751 . . 771 774 + SetI ASST 774 770 . . 771 774 + CviJI RGCY 772 772 . . 771 774 + AluI AGCT 772 772 . . 767 772 - Eco57MI CTGRAG 752 750 . . 767 772 - Eco57I CTGAAG 752 750 . . 777 782 + Hpy178III* TCNNGA 778 780 . . 791 794 + TspEI AATT 790 794 . . 795 798 + CviJI RGCY 796 796 . . 797 800 + MnlI CCTC 807 806 . . 802 805 + CviRI* TGCA 803 803 . . 808 812 + Hpy188I TCNGA 810 809 . . 812 816 + TspDTI ATGAA 827 825 . . 828 832 + PleI GAGTC 836 837 . . 828 832 + MlyI GAGTC 837 837 . . 828 832 + HinfI GANTC 828 831 . . 831 836 + Hpy178III* TCNNGA 832 834 . . 831 836 + BetI* WCCGGW 831 835 . . 831 836 + BspMII* TCCGGA 831 835 . . 832 835 + HpaII CCGG 832 834 . . 841 845 + FauI CCCGC 849 851 . . 842 845 + AciI CCGC 842 844 . . 868 871 + SetI ASST 871 867 . . 882 891 + BsaBI GATNNNNATC 886 886 . . 887 891 + SfaNI GCATC 896 900 . . 888 892 - FokI GGATG 874 878 . . 888 892 - BseGI GGATG 887 885 . . 890 894 - TspGWI ACGGA 880 878 . . 903 906 + FatI CATG 902 906 . . 903 906 + NlaIII CATG 906 902 . . 903 906 + CviAII CATG 903 905 . . 905 908 + CviRI* TGCA 906 906 . . 909 913 + Tsp45I GTSAC 908 913 . . 909 913 + MaeIII GTNAC 908 913 . . 910 914 - HphI GGTGA 902 901 . . 916 919 + MseI TTAA 916 918 . . 922 926 + TfiI GAWTC 922 925 . . 922 926 + HinfI GANTC 922 925 . . 935 940 + ApoI RAATTY 935 939 . . 936 939 + TspEI AATT 935 939 . . 943 948 + AsuII TTCGAA 944 946 . . 944 947 + TaqI TCGA 944 946 . . 941 945 - MboII GAAGA 933 932 . . 952 957 + TatI WGTACW 952 956 . . 952 957 + ScaI AGTACT 954 954 . . 953 956 + Csp6I GTAC 953 955 . . 953 956 + RsaI GTAC 954 954 . . 966 970 + Tsp4CI* ACNGT 968 967 . . 973 977 + HphI GGTGA 985 984 . . 984 993 + XmnI GAANNNNTTC 988 988 . . 1010 1013 + TaiI ACGT 1013 1009 . . 1010 1013 + SetI ASST 1013 1009 . . 1010 1013 + MaeII ACGT 1010 1012 . . 1020 1025 + SecI* CCNNGG 1020 1024 . . 1021 1025 + BssKI CCNGG 1020 1025 . . 1021 1025 + BseBI CCWGG 1022 1023 . . 1021 1025 + ScrFI CCNGG 1022 1023 . . 1021 1025 + EcoRII CCWGG 1020 1025 . . 1033 1037 + Hpy188I TCNGA 1035 1034 . . 1032 1037 - MmeI TCCRAC 1013 1011 . . 1047 1051 - BceAI ACGGC 1032 1034 . . 1063 1066 + TspEI AATT 1062 1066 . . 1077 1080 + SetI ASST 1080 1076 . . 1077 1080 + CviJI RGCY 1078 1078 . . 1077 1080 + AluI AGCT 1078 1078 . . 1084 1088 + TspGWI ACGGA 1099 1097 . . 1091 1096 + MfeI CAATTG 1091 1095 . . 1092 1095 + TspEI AATT 1091 1095 . . 1095 1098 + CviRI* TGCA 1096 1096 . . 1097 1100 + FatI CATG 1096 1100 . . 1097 1100 + NlaIII CATG 1100 1096 . . 1097 1100 + CviAII CATG 1097 1099 . . 1107 1110 + MnlI CCTC 1117 1116 . . 1103 1107 - FokI GGATG 1089 1093 . . 1103 1107 - BseGI GGATG 1102 1100 . . 1130 1133 + TspEI AATT 1129 1133 . . 1136 1140 + Hpy188I TCNGA 1138 1137 . . 1141 1144 + FatI CATG 1140 1144 . . 1141 1144 + NlaIII CATG 1144 1140 . . 1141 1144 + CviAII CATG 1141 1143 . . 1147 1150 + MseI TTAA 1147 1149 . . 1156 1160 + BlsI GCNGC 1158 1157 . . 1156 1160 + BisI GCNGC 1157 1158 . . 1156 1160 + TseI GCWGC 1156 1159 . . 1159 1162 + Hin6I GCGC 1159 1161 . . 1159 1162 + GlaI GCGC 1160 1160 . . 1159 1162 + HhaI GCGC 1161 1159 . . 1156 1160 - BbvI GCAGC 1143 1147 . . 1166 1170 + Hpy188I TCNGA 1168 1167 . . 1170 1175 + ApoI RAATTY 1170 1174 . . 1171 1174 + TspEI AATT 1170 1174 . . 1176 1180 - MboII GAAGA 1168 1167 . . 1180 1183 + MaeI CTAG 1180 1182 . . 1181 1186 + TsoI TARCCA 1197 1195 . . 1182 1185 + CviJI RGCY 1183 1183 . . 1198 1203 + DsaI* CCRYGG 1198 1202 . . 1198 1203 + SecI* CCNNGG 1198 1202 . . 1197 1201 - BceAI ACGGC 1182 1184 . . 1203 1206 + Csp6I GTAC 1203 1205 . . 1203 1206 + RsaI GTAC 1204 1204 . . 1206 1211 + MfeI CAATTG 1206 1210 . . 1207 1210 + TspEI AATT 1206 1210 . . 1212 1216 + BccI CCATC 1220 1221 . . 1213 1217 - FokI GGATG 1199 1203 . . 1213 1217 - BseGI GGATG 1212 1210 . . 1222 1226 + Hpy188I TCNGA 1224 1223 . . 1225 1228 + DpnI GATC 1226 1226 . . 1225 1228 + BstKTI GATC 1227 1225 . . 1225 1228 + MboI GATC 1224 1228 . . 1227 1232 + Hpy178III* TCNNGA 1228 1230 . . 1225 1229 - BinI* GGATC 1219 1220 . . 1232 1235 + TspEI AATT 1231 1235 . . 1247 1252 + TsoI TARCCA 1263 1261 . . 1255 1259 - FokI GGATG 1241 1245 . . 1255 1259 - BseGI GGATG 1254 1252 . . 1280 1285 + TsoI TARCCA 1296 1294 . . 1281 1284 + CviJI RGCY 1282 1282 . . 1283 1288 + DsaI* CCRYGG 1283 1287 . . 1283 1288 + StyI CCWWGG 1283 1287 . . 1283 1288 + NcoI CCATGG 1283 1287 . . 1283 1288 + SecI* CCNNGG 1283 1287 . . 1284 1287 + FatI CATG 1283 1287 . . 1284 1287 + NlaIII CATG 1287 1283 . . 1284 1287 + CviAII CATG 1284 1286 . . 1287 1291 + FokI GGATG 1300 1304 . . 1287 1291 + BseGI GGATG 1293 1291 . . 1289 1293 + TspDTI ATGAA 1304 1302 . . 1293 1298 + TatI WGTACW 1293 1297 . . 1293 1298 + ScaI AGTACT 1295 1295 . . 1294 1297 + Csp6I GTAC 1294 1296 . . 1294 1297 + RsaI GTAC 1295 1295 . . 1315 1318 + DpnI GATC 1316 1316 . . 1315 1318 + BstKTI GATC 1317 1315 . . 1315 1318 + MboI GATC 1314 1318 . . 1317 1321 + Hpy188I TCNGA 1319 1318 . . 1327 1337 + MwoI GCNNNNNNNGC 1333 1330 . . 1330 1333 + MnlI CCTC 1340 1339 . . 1333 1337 + FauI CCCGC 1341 1343 . . 1328 1333 - BseRI GAGGAG 1319 1317 . . 1334 1337 + AciI CCGC 1334 1336 . . 1344 1348 + DdeI CTNAG 1344 1347 . . 1350 1354 + PleI GAGTC 1358 1359 . . 1350 1354 + MlyI GAGTC 1359 1359 . . 1350 1354 + HinfI GANTC 1350 1353 . . 1353 1357 + Hpy188I TCNGA 1355 1354 . . 1359 1363 + TfiI GAWTC 1359 1362 . . 1359 1363 + HinfI GANTC 1359 1362 . . 1378 1381 + SetI ASST 1381 1377 . . 1394 1398 + BlsI GCNGC 1396 1395 . . 1394 1398 + BisI GCNGC 1395 1396 . . 1394 1398 + BbvI GCAGC 1406 1410 . . 1394 1398 + TseI GCWGC 1394 1397 . . 1396 1399 + SetI ASST 1399 1395 . . 1396 1399 + CviJI RGCY 1397 1397 . . 1396 1399 + AluI AGCT 1397 1397 . . 1400 1404 + DdeI CTNAG 1400 1403 . . 1405 1408 + TaqI TCGA 1405 1407 . . 1407 1412 + BsmI GAATGC 1413 1411 . . 1425 1428 + CviJI RGCY 1426 1426 . . 1427 1430 + HpaII CCGG 1427 1429 . . 1447 1450 + TaqI TCGA 1447 1449 . . 1447 1452 + Hpy178III* TCNNGA 1448 1450 . . 1455 1458 + CviRI* TGCA 1456 1456 . . 1456 1461 + BtsI GCAGTG 1463 1461 . . 1457 1463 + TspRI CASTGNN 1463 1454 . . 1460 1465 + BalI TGGCCA 1462 1462 . . 1460 1465 + CfrI YGGCCR 1460 1464 . . 1461 1464 + CviJI RGCY 1462 1462 . . 1461 1464 + HaeIII GGCC 1462 1462 . . 1465 1468 + TspEI AATT 1464 1468 . . 1468 1471 + MseI TTAA 1468 1470 . . 1471 1476 + TatI WGTACW 1471 1475 . . 1472 1475 + Csp6I GTAC 1472 1474 . . 1472 1475 + RsaI GTAC 1473 1473 . . 1479 1483 + BsmAI GTCTC 1484 1488 . . 1483 1486 + MaeI CTAG 1483 1485 . . 1507 1512 + Hpy178III* TCNNGA 1508 1510 . . 1526 1529 + CviRI* TGCA 1527 1527 . . 1531 1534 + SetI ASST 1534 1530 . . 1531 1534 + CviJI RGCY 1532 1532 . . 1531 1534 + AluI AGCT 1532 1532 . . 1544 1547 + TspEI AATT 1543 1547 . . 1549 1554 + EcoP15I CAGCAG 1579 1581 . . 1551 1555 + BlsI GCNGC 1553 1552 . . 1551 1555 + BisI GCNGC 1552 1553 . . 1551 1555 + BbvI GCAGC 1563 1567 . . 1551 1555 + TseI GCWGC 1551 1554 . . 1552 1557 + EcoP15I CAGCAG 1582 1584 . . 1554 1558 + BlsI GCNGC 1556 1555 . . 1554 1558 + BisI GCNGC 1555 1556 . . 1554 1558 + BbvI GCAGC 1566 1570 . . 1554 1558 + TseI GCWGC 1554 1557 . . 1574 1577 + TspEI AATT 1573 1577 . . 1596 1601 + ApoI RAATTY 1596 1600 . . 1597 1600 + TspEI AATT 1596 1600 . . 1613 1616 + TspEI AATT 1612 1616 . . 1640 1644 + Hpy188I TCNGA 1642 1641 . . 1647 1651 + FauI CCCGC 1655 1657 . . 1648 1651 + AciI CCGC 1648 1650 . . 1650 1660 + MwoI GCNNNNNNNGC 1656 1653 . . 1651 1657 + Bpu10I CCTNAGC 1652 1655 . . 1652 1656 + DdeI CTNAG 1652 1655 . . 1652 1656 - BspCNI CTCAG 1644 1642 . . 1652 1656 - BseMII CTCAG 1643 1641 . . 1656 1660 + BlsI GCNGC 1658 1657 . . 1656 1660 + BisI GCNGC 1657 1658 . . 1656 1660 + BbvI GCAGC 1668 1672 . . 1656 1660 + TseI GCWGC 1656 1659 . . 1658 1661 + SetI ASST 1661 1657 . . 1658 1661 + CviJI RGCY 1659 1659 . . 1658 1661 + AluI AGCT 1659 1659 . . 1676 1679 + SetI ASST 1679 1675 . . 1680 1684 - MboII GAAGA 1672 1671 . . 1694 1697 + CviRI* TGCA 1695 1695 . . 1695 1699 + SfaNI GCATC 1704 1708 . . 1700 1705 + Hpy166II GTNNAC 1702 1702 . . 1700 1705 + BssNAI GTATAC 1702 1702 . . 1689 1700 - BcgI CGANNNNNNTGC 1712 1710 1678 1676 1700 1705 + AccI GTMKAC 1701 1703 . . 1703 1708 + BsaAI YACGTR 1705 1705 . . 1704 1707 + TaiI ACGT 1707 1703 . . 1704 1707 + SetI ASST 1707 1703 . . 1704 1707 + MaeII ACGT 1704 1706 . . 1714 1717 + Csp6I GTAC 1714 1716 . . 1714 1717 + RsaI GTAC 1715 1715 . . 1723 1728 + Hpy178III* TCNNGA 1724 1726 . . 1741 1744 + CviJI RGCY 1742 1742 . . 1741 1744 + HaeIII GGCC 1742 1742 . . 1741 1745 + BmgT120I GGNCC 1742 1743 . . 1741 1745 + AsuI* GGNCC 1741 1744 . . 1744 1749 + DsaI* CCRYGG 1744 1748 . . 1744 1749 + SecI* CCNNGG 1744 1748 . . 1749 1752 + CviJI RGCY 1750 1750 . . 1753 1758 + Hpy166II GTNNAC 1755 1755 . . 1753 1758 + AccI GTMKAC 1754 1756 . . 1757 1760 + SetI ASST 1760 1756 . . 1761 1765 + Tsp45I GTSAC 1760 1765 . . 1761 1765 + MaeIII GTNAC 1760 1765 . . 1773 1776 + SetI ASST 1776 1772 . . 1778 1782 - MboII GAAGA 1770 1769 . . 1787 1790 + TaiI ACGT 1790 1786 . . 1787 1790 + SetI ASST 1790 1786 . . 1787 1790 + MaeII ACGT 1787 1789 . . 1796 1799 + SetI ASST 1799 1795 . . 1796 1799 + CviJI RGCY 1797 1797 . . 1796 1799 + AluI AGCT 1797 1797 . . 1797 1801 + BlsI GCNGC 1799 1798 . . 1797 1801 + BisI GCNGC 1798 1799 . . 1797 1801 + TseI GCWGC 1797 1800 . . 1797 1801 - BbvI GCAGC 1784 1788 . . 1811 1819 + AlwNI CAGNNNCTG 1816 1813 . . 1815 1818 + SetI ASST 1818 1814 . . 1817 1821 + DdeI CTNAG 1817 1820 . . 1817 1821 - BspCNI CTCAG 1809 1807 . . 1817 1821 - BseMII CTCAG 1808 1806 . . 1826 1829 + CviJI RGCY 1827 1827 . . 1829 1833 + BccI CCATC 1837 1838 . . 1830 1834 - FokI GGATG 1816 1820 . . 1830 1834 - BseGI GGATG 1829 1827 . . 1834 1837 + MaeI CTAG 1834 1836 . . 1847 1852 + SduI GDGCHC 1851 1847 . . 1847 1852 + Bsp120I GGGCCC 1847 1851 . . 1847 1852 + NlaIV GGNNCC 1849 1849 . . 1847 1851 + BmgT120I GGNCC 1848 1849 . . 1847 1852 + HgiJII* GRGCYC 1851 1847 . . 1847 1852 + BseSI GKGCMC 1851 1847 . . 1847 1851 + AsuI* GGNCC 1847 1850 . . 1847 1852 + ApaI GGGCCC 1851 1847 . . 1848 1851 + CviJI RGCY 1849 1849 . . 1848 1851 + HaeIII GGCC 1849 1849 . . 1848 1852 + BmgT120I GGNCC 1849 1850 . . 1848 1852 + AsuI* GGNCC 1848 1851 . . 1857 1860 + FatI CATG 1856 1860 . . 1857 1860 + NlaIII CATG 1860 1856 . . 1857 1860 + CviAII CATG 1857 1859 . . 1889 1892 + TspEI AATT 1888 1892 . . 1890 1895 + VspI ATTAAT 1891 1893 . . 1891 1894 + MseI TTAA 1891 1893 . . 1921 1925 + MaeIII GTNAC 1920 1925 . . 1924 1928 + Tsp4CI* ACNGT 1926 1925 . . 1925 1931 + TspRI CASTGNN 1931 1922 . . 1933 1936 + Csp6I GTAC 1933 1935 . . 1933 1936 + RsaI GTAC 1934 1934 . . 1935 1938 + SetI ASST 1938 1934 . . 1936 1946 + BsiYI* CCNNNNNNNGG 1942 1939 . . 1949 1952 + MseI TTAA 1949 1951 . . 1955 1960 + NspI RCATGY 1959 1955 . . 1956 1959 + FatI CATG 1955 1959 . . 1956 1959 + NlaIII CATG 1959 1955 . . 1956 1959 + CviAII CATG 1956 1958 . . 1962 1965 + SetI ASST 1965 1961 . . 1962 1965 + CviJI RGCY 1963 1963 . . 1962 1965 + AluI AGCT 1963 1963 . . 1972 1975 + CviJI RGCY 1973 1973 . . 1973 1979 + EspI* GCTNAGC 1974 1977 . . 1974 1978 + DdeI CTNAG 1974 1977 . . 1974 1978 + BspCNI CTCAG 1987 1985 . . 1974 1978 + BseMII CTCAG 1988 1986 . . 1977 1980 + CviJI RGCY 1978 1978 . . 1980 1983 + FatI CATG 1979 1983 . . 1980 1983 + NlaIII CATG 1983 1979 . . 1980 1983 + CviAII CATG 1980 1982 . . 1994 1999 + NlaIV GGNNCC 1996 1996 . . 2001 2006 + Hpy166II GTNNAC 2003 2003 . . 2001 2006 + HindII GTYRAC 2003 2003 . . 2013 2016 + CviRI* TGCA 2014 2014 . . 2014 2018 + SfaNI GCATC 2023 2027 . . 2015 2020 - BtgZI GCGATG 2000 2004 . . 2032 2036 + BmgT120I GGNCC 2033 2034 . . 2032 2036 + AvaII GGWCC 2032 2035 . . 2032 2036 + AsuI* GGNCC 2032 2035 . . 2035 2039 + BccI CCATC 2043 2044 . . 2041 2046 + Hpy178III* TCNNGA 2042 2044 . . 2038 2042 - MboII GAAGA 2030 2029 . . 2051 2055 + TspDTI ATGAA 2066 2064 . . 2057 2062 + Cac8I GCNNGC 2059 2059 . . 2077 2082 + MmeI TCCRAC 2102 2100 . . 2075 2079 - FokI GGATG 2061 2065 . . 2075 2079 - BseGI GGATG 2074 2072 . . 2085 2089 + BmgT120I GGNCC 2086 2087 . . 2085 2089 + AsuI* GGNCC 2085 2088 . . 2086 2089 + CviJI RGCY 2087 2087 . . 2086 2089 + HaeIII GGCC 2087 2087 . . 2097 2101 - MboII GAAGA 2089 2088 . . 2110 2119 + MslI CAYNNNNRTG 2114 2114 . . 2129 2133 + TspDTI ATGAA 2144 2142 . . 2139 2142 + SetI ASST 2142 2138 . . 2139 2142 + CviJI RGCY 2140 2140 . . 2139 2142 + AluI AGCT 2140 2140 . . 2145 2148 + CviJI RGCY 2146 2146 . . 2161 2165 + SfaNI GCATC 2170 2174 . . 2171 2176 + AsuII TTCGAA 2172 2174 . . 2172 2175 + TaqI TCGA 2172 2174 . . 2182 2185 + MnlI CCTC 2192 2191 . . 2197 2200 + TspEI AATT 2196 2200 . . 2214 2218 + BsrI ACTGG 2219 2217 . . 2218 2223 + Hpy166II GTNNAC 2220 2220 . . 2218 2223 + HindII GTYRAC 2220 2220 . . 2224 2234 + FalI AAGNNNNNCTT 2215 2210 2247 2242 2224 2234 - FalI AAGNNNNNCTT 2247 2242 2215 2210 2245 2248 + TspEI AATT 2244 2248 . . 2248 2252 + Hpy188I TCNGA 2250 2249 . . 2249 2253 + DdeI CTNAG 2249 2252 . . 2249 2253 - BspCNI CTCAG 2241 2239 . . 2249 2253 - BseMII CTCAG 2240 2238 . . 2263 2267 + DdeI CTNAG 2263 2266 . . 2268 2272 + Tsp4CI* ACNGT 2270 2269 . . 2269 2275 + TspRI CASTGNN 2275 2266 . . 2289 2292 + SetI ASST 2292 2288 . . 2312 2322 + EcoNI CCTNNNNNAGG 2316 2317 . . 2312 2322 + BsiYI* CCNNNNNNNGG 2318 2315 . . 2314 2317 + MseI TTAA 2314 2316 . . 2320 2323 + SetI ASST 2323 2319 . . 2332 2336 + TspDTI ATGAA 2347 2345 . . 2338 2342 + BsrI ACTGG 2343 2341 . . 2352 2357 + SspI AATATT 2354 2354 . . 2372 2378 + PpuMI RGGWCCY 2373 2376 . . 2372 2378 + DraII RGGNCCY 2373 2376 . . 2373 2378 + NlaIV GGNNCC 2375 2375 . . 2373 2377 + BmgT120I GGNCC 2374 2375 . . 2373 2377 + AvaII GGWCC 2373 2376 . . 2373 2377 + AsuI* GGNCC 2373 2376 . . 2370 2374 - Hin4II* CCTTC 2364 2363 . . 2380 2383 + Csp6I GTAC 2380 2382 . . 2380 2383 + RsaI GTAC 2381 2381 . . 2382 2385 + SetI ASST 2385 2381 . . 2384 2389 + Eco57MI CTGRAG 2405 2403 . . 2384 2389 + Eco57I CTGAAG 2405 2403 . . 2393 2402 + XmnI GAANNNNTTC 2397 2397 . . 2397 2400 + SetI ASST 2400 2396 . . 2397 2400 + CviJI RGCY 2398 2398 . . 2397 2400 + AluI AGCT 2398 2398 . . 2409 2412 + CviRI* TGCA 2410 2410 . . 2414 2425 + BdaI TGANNNNNNTCA 2403 2401 2437 2435 2415 2424 + XmnI GAANNNNTTC 2419 2419 . . 2414 2425 - BdaI TGANNNNNNTCA 2437 2435 2403 2401 2422 2426 - TspDTI ATGAA 2412 2410 . . 2428 2433 + Hpy178III* TCNNGA 2429 2431 . . 2436 2441 + Hpy178III* TCNNGA 2437 2439 . . 2442 2445 + CviRI* TGCA 2443 2443 . . 2440 2444 - SfaNI GCATC 2430 2434 . . 2447 2450 + SetI ASST 2450 2446 . . 2446 2449 - MnlI CCTC 2439 2438 . . 2451 2454 + TspEI AATT 2450 2454 . . 2462 2465 + CviRI* TGCA 2463 2463 . . 2470 2475 + ApoI RAATTY 2470 2474 . . 2471 2474 + TspEI AATT 2470 2474 . . 2476 2480 + MboII GAAGA 2488 2487 . . 2481 2485 + Tsp4CI* ACNGT 2483 2482 . . 2492 2495 + TspEI AATT 2491 2495 . . 2502 2512 + MwoI GCNNNNNNNGC 2508 2505 . . 2504 2507 + TspEI AATT 2503 2507 . . 2508 2518 + BsaXI ACNNNNNCTCC 2498 2495 2528 2525 2511 2515 + SfaNI GCATC 2520 2524 . . 2529 2533 + BccI CCATC 2537 2538 . . 2544 2548 + Tsp4CI* ACNGT 2546 2545 . . 2549 2554 + GsaI CCCAGC 2553 2549 . . 2549 2554 + BseYI CCCAGC 2549 2553 . . 2547 2551 - BslFI GGGAC 2532 2536 . . 2553 2563 + MwoI GCNNNNNNNGC 2559 2556 . . 2557 2562 + EcoP15I CAGCAG 2587 2589 . . 2559 2563 + BlsI GCNGC 2561 2560 . . 2559 2563 + BisI GCNGC 2560 2561 . . 2559 2563 + BbvI GCAGC 2571 2575 . . 2559 2563 + TseI GCWGC 2559 2562 . . 2560 2565 + EcoP15I CAGCAG 2590 2592 . . 2562 2566 + BlsI GCNGC 2564 2563 . . 2562 2566 + BisI GCNGC 2563 2564 . . 2562 2566 + BbvI GCAGC 2574 2578 . . 2562 2566 + TseI GCWGC 2562 2565 . . 2565 2569 + SfaNI GCATC 2574 2578 . . 2565 2575 + MwoI GCNNNNNNNGC 2571 2568 . . 2568 2573 + Hpy178III* TCNNGA 2569 2571 . . 2569 2574 + SmlI CTYRAG 2569 2573 . . 2569 2574 + Bce83I* CTTGAG 2590 2588 . . 2574 2578 + BlsI GCNGC 2576 2575 . . 2574 2578 + BisI GCNGC 2575 2576 . . 2574 2578 + BbvI GCAGC 2586 2590 . . 2574 2578 + TseI GCWGC 2574 2577 . . 2575 2580 + EcoP15I CAGCAG 2605 2607 . . 2577 2581 + BlsI GCNGC 2579 2578 . . 2577 2581 + BisI GCNGC 2578 2579 . . 2577 2581 + BbvI GCAGC 2589 2593 . . 2577 2581 + TseI GCWGC 2577 2580 . . 2578 2583 + EcoP15I CAGCAG 2608 2610 . . 2584 2588 + TspDTI ATGAA 2599 2597 . . 2589 2592 + TspEI AATT 2588 2592 . . 2601 2605 + TfiI GAWTC 2601 2604 . . 2601 2605 + HinfI GANTC 2601 2604 . . 2621 2624 + CviRI* TGCA 2622 2622 . . 2632 2635 + MaeI CTAG 2632 2634 . . 2649 2652 - MnlI CCTC 2642 2641 . . 2656 2659 + MseI TTAA 2656 2658 . . 2670 2673 + TspEI AATT 2669 2673 . . 2682 2685 + DpnI GATC 2683 2683 . . 2682 2685 + BstKTI GATC 2684 2682 . . 2682 2691 + BsaBI GATNNNNATC 2686 2686 . . 2682 2685 + MboI GATC 2681 2685 . . 2694 2697 + SetI ASST 2697 2693 . . 2698 2701 + MseI TTAA 2698 2700 . . 2707 2717 + Hin4I GAYNNNNNVTC 2698 2693 2730 2725 2707 2711 - BccI CCATC 2701 2702 . . 2712 2716 - TspDTI ATGAA 2702 2700 . . 2707 2717 - Hin4I GAYNNNNNVTC 2730 2725 2698 2693 2723 2727 - TspGWI ACGGA 2713 2711 . . 2733 2738 + SpeI ACTAGT 2733 2737 . . 2734 2737 + MaeI CTAG 2734 2736 . . 2760 2764 + PleI GAGTC 2768 2769 . . 2760 2764 + MlyI GAGTC 2769 2769 . . 2760 2764 + HinfI GANTC 2760 2763 . . 2765 2769 + MaeIII GTNAC 2764 2769 . . 2809 2813 + MboII GAAGA 2821 2820 . . 2805 2810 - MmeI TCCRAC 2786 2784 . . 2836 2841 + EcoRV GATATC 2838 2838 . . 2847 2850 + TaqI TCGA 2847 2849 . . 2849 2858 + XmnI GAANNNNTTC 2853 2853 . . 2857 2861 + Hpy188I TCNGA 2859 2858 . . 2881 2886 + NlaIV GGNNCC 2883 2883 . . 2895 2901 + TspRI CASTGNN 2901 2892 . . 2896 2900 + Tsp4CI* ACNGT 2898 2897 . . 2912 2917 + Hpy178III* TCNNGA 2913 2915 . . 2916 2920 + TfiI GAWTC 2916 2919 . . 2916 2920 + HinfI GANTC 2916 2919 . . 2920 2923 + MaeI CTAG 2920 2922 . . 2922 2925 + CviJI RGCY 2923 2923 . . 2926 2930 + MboII GAAGA 2938 2937 . . 2928 2933 + XhoII RGATCY 2928 2932 . . 2929 2932 + DpnI GATC 2930 2930 . . 2929 2932 + BstKTI GATC 2931 2929 . . 2929 2932 + MboI GATC 2928 2932 . . 2929 2933 - BinI* GGATC 2923 2924 . . 2951 2954 + TspEI AATT 2950 2954 . . 2963 2966 + SetI ASST 2966 2962 . . 2961 2966 - BspMI ACCTGC 2952 2956 . . 2987 2990 + TspEI AATT 2986 2990 . . 2989 2992 + MseI TTAA 2989 2991 . . 2992 2997 + HaeII RGCGCY 2996 2992 . . 2993 2996 + Hin6I GCGC 2993 2995 . . 2993 2996 + GlaI GCGC 2994 2994 . . 2993 2996 + HhaI GCGC 2995 2993 . . 2997 3011 + XcmI CCANNNNNNNNNTGG 3004 3003 . . 3011 3016 + Hpy166II GTNNAC 3013 3013 . . 3013 3017 + BmgT120I GGNCC 3014 3015 . . 3013 3017 + AvaII GGWCC 3013 3016 . . 3013 3017 + AsuI* GGNCC 3013 3016 . . 3021 3024 + CviRI* TGCA 3022 3022 . . 3034 3039 + ApoI RAATTY 3034 3038 . . 3035 3038 + TspEI AATT 3034 3038 . . 3052 3055 + SetI ASST 3055 3051 . . 3052 3055 + CviJI RGCY 3053 3053 . . 3052 3055 + AluI AGCT 3053 3053 . . 3055 3058 + TaqI TCGA 3055 3057 . . 3060 3063 + SetI ASST 3063 3059 . . 3060 3063 + CviJI RGCY 3061 3061 . . 3060 3063 + AluI AGCT 3061 3061 . . 3063 3067 - HphI GGTGA 3055 3054 . . 3070 3074 + BsrI ACTGG 3075 3073 . . 3076 3081 + ApoI RAATTY 3076 3080 . . 3077 3080 + TspEI AATT 3076 3080 . . 3086 3089 + MseI TTAA 3086 3088 . . 3088 3091 + TspEI AATT 3087 3091 . . 3091 3095 - MboII GAAGA 3083 3082 . . 3098 3101 + SetI ASST 3101 3097 . . 3096 3101 - BspMI ACCTGC 3087 3091 . . 3104 3107 + TspEI AATT 3103 3107 . . 3117 3121 - TspDTI ATGAA 3107 3105 . . 3125 3128 + DpnI GATC 3126 3126 . . 3125 3128 + BstKTI GATC 3127 3125 . . 3125 3128 + MboI GATC 3124 3128 . . 3133 3137 - HphI GGTGA 3125 3124 . . 3144 3147 - MnlI CCTC 3137 3136 . . 3158 3163 + EcoT22I ATGCAT 3162 3158 . . 3159 3162 + CviRI* TGCA 3160 3160 . . 3160 3165 - BsmI GAATGC 3160 3158 . . 3174 3184 + MwoI GCNNNNNNNGC 3180 3177 . . 3184 3187 + FatI CATG 3183 3187 . . 3184 3187 + NlaIII CATG 3187 3183 . . 3184 3187 + CviAII CATG 3184 3186 . . 3195 3205 + Hin4I GAYNNNNNVTC 3186 3181 3218 3213 3195 3200 + EcoRV GATATC 3197 3197 . . 3195 3205 - Hin4I GAYNNNNNVTC 3218 3213 3186 3181 3202 3206 - FokI GGATG 3188 3192 . . 3202 3206 - BseGI GGATG 3201 3199 . . 3210 3213 - AciI CCGC 3210 3212 . . 3222 3225 + SetI ASST 3225 3221 . . 3222 3225 + CviJI RGCY 3223 3223 . . 3222 3225 + AluI AGCT 3223 3223 . . 3233 3238 + Hpy178III* TCNNGA 3234 3236 . . 3234 3245 + BstXI CCANNNNNNTGG 3241 3237 . . 3237 3241 + PleI GAGTC 3245 3246 . . 3237 3241 + MlyI GAGTC 3246 3246 . . 3237 3241 + HinfI GANTC 3237 3240 . . 3253 3257 + TfiI GAWTC 3253 3256 . . 3253 3257 + HinfI GANTC 3253 3256 . . 3263 3267 + Tsp45I GTSAC 3262 3267 . . 3263 3267 + MaeIII GTNAC 3262 3267 . . 3265 3269 + HinfI GANTC 3265 3268 . . 3268 3272 + Hpy188I TCNGA 3270 3269 . . 3265 3269 - PleI GAGTC 3259 3260 . . 3265 3269 - MlyI GAGTC 3259 3259 . . 3271 3281 + Hin4I GAYNNNNNVTC 3262 3257 3294 3289 3271 3281 - Hin4I GAYNNNNNVTC 3294 3289 3262 3257 3292 3295 + TaqI TCGA 3292 3294 . . 3295 3299 + TspDTI ATGAA 3310 3308 . . 3307 3310 + SetI ASST 3310 3306 . . 3308 3311 + MnlI CCTC 3318 3317 . . 3318 3324 + SanDI GGGWCCC 3319 3322 . . 3318 3324 + PpuMI RGGWCCY 3319 3322 . . 3318 3324 + DraII RGGNCCY 3319 3322 . . 3318 3323 + NlaIV GGNNCC 3320 3320 . . 3319 3324 + NlaIV GGNNCC 3321 3321 . . 3319 3323 + BmgT120I GGNCC 3320 3321 . . 3319 3323 + AvaII GGWCC 3319 3322 . . 3319 3323 + AsuI* GGNCC 3319 3322 . . 3323 3326 + MnlI CCTC 3333 3332 . . 3323 3328 + SecI* CCNNGG 3323 3327 . . 3320 3324 - BslFI GGGAC 3305 3309 . . 3324 3329 + BmeT110I CYCGRG 3325 3327 . . 3324 3329 + AvaI CYCGRG 3324 3328 . . 3328 3331 + CviJI RGCY 3329 3329 . . 3355 3360 + SspI AATATT 3357 3357 . . 3365 3370 + SfeI* CTRYAG 3365 3369 . . 3368 3373 + PvuII CAGCTG 3370 3370 . . 3368 3373 + NspBII* CMGCKG 3370 3370 . . 3369 3372 + SetI ASST 3372 3368 . . 3369 3372 + CviJI RGCY 3370 3370 . . 3369 3372 + AluI AGCT 3370 3370 . . 3371 3375 + DdeI CTNAG 3371 3374 . . 3375 3379 + HinfI GANTC 3375 3378 . . 3371 3375 - BspCNI CTCAG 3363 3361 . . 3371 3375 - BseMII CTCAG 3362 3360 . . 3373 3377 - BsmAI GTCTC 3367 3371 . . 3375 3379 - PleI GAGTC 3369 3370 . . 3375 3379 - MlyI GAGTC 3369 3369 . . 3383 3387 + TfiI GAWTC 3383 3386 . . 3383 3387 + HinfI GANTC 3383 3386 . . 3386 3391 + Hpy178III* TCNNGA 3387 3389 . . 3386 3391 + BetI* WCCGGW 3386 3390 . . 3386 3391 + BspMII* TCCGGA 3386 3390 . . 3387 3390 + HpaII CCGG 3387 3389 . . 3409 3414 + MfeI CAATTG 3409 3413 . . 3410 3413 + TspEI AATT 3409 3413 . . 3418 3421 + MseI TTAA 3418 3420 . . 3443 3448 + BsaAI YACGTR 3445 3445 . . 3443 3448 + PmaCI CACGTG 3445 3445 . . 3444 3447 + TaiI ACGT 3447 3443 . . 3444 3447 + SetI ASST 3447 3443 . . 3444 3447 + MaeII ACGT 3444 3446 . . 3447 3458 + BdaI TGANNNNNNTCA 3436 3434 3470 3468 3448 3457 + XmnI GAANNNNTTC 3452 3452 . . 3449 3454 + SspI AATATT 3451 3451 . . 3456 3461 + Hpy178III* TCNNGA 3457 3459 . . 3447 3458 - BdaI TGANNNNNNTCA 3470 3468 3436 3434 3469 3473 + TspDTI ATGAA 3484 3482 . . 3483 3488 + EciI GGCGGA 3499 3497 . . 3481 3484 - MnlI CCTC 3474 3473 . . 3486 3490 + FokI GGATG 3499 3503 . . 3486 3490 + BseGI GGATG 3492 3490 . . 3484 3487 - AciI CCGC 3484 3486 . . 3488 3492 + TspDTI ATGAA 3503 3501 . . 3492 3495 + SetI ASST 3495 3491 . . 3493 3497 + BssKI CCNGG 3492 3497 . . 3493 3497 + BseBI CCWGG 3494 3495 . . 3493 3497 + ScrFI CCNGG 3494 3495 . . 3493 3497 + EcoRII CCWGG 3492 3497 . . 3497 3502 + BtsI GCAGTG 3504 3502 . . 3498 3504 + TspRI CASTGNN 3504 3495 . . 3515 3518 + MaeI CTAG 3515 3517 . . 3528 3531 + TaiI ACGT 3531 3527 . . 3528 3531 + SetI ASST 3531 3527 . . 3528 3531 + MaeII ACGT 3528 3530 . . 3525 3529 - BsmAI GTCTC 3519 3523 . . 3530 3534 + MaeIII GTNAC 3529 3534 . . 3525 3530 - Esp3I CGTCTC 3519 3523 . . 3539 3543 + TspDTI ATGAA 3554 3552 . . 3551 3557 + PfoI TCCNGGA 3551 3556 . . 3552 3556 + BssKI CCNGG 3551 3556 . . 3552 3556 + BseBI CCWGG 3553 3554 . . 3552 3556 + ScrFI CCNGG 3553 3554 . . 3552 3556 + EcoRII CCWGG 3551 3556 . . 3575 3578 + TspEI AATT 3574 3578 . . 3579 3584 + ClaI ATCGAT 3580 3582 . . 3580 3583 + TaqI TCGA 3580 3582 . . 3592 3595 + TspEI AATT 3591 3595 . . 3596 3600 + BmgT120I GGNCC 3597 3598 . . 3596 3600 + AvaII GGWCC 3596 3599 . . 3596 3600 + AsuI* GGNCC 3596 3599 . . 3605 3608 + TspEI AATT 3604 3608 . . 3615 3620 + AhaIII* TTTAAA 3617 3617 . . 3616 3619 + MseI TTAA 3616 3618 . . 3629 3634 + Hpy166II GTNNAC 3631 3631 . . 3629 3634 + HindII GTYRAC 3631 3631 . . 3643 3647 + TspDTI ATGAA 3658 3656 . . 3654 3658 - BsrI ACTGG 3654 3652 . . 3653 3658 - BfiI ACTGGG 3648 3647 . . 3661 3665 + MboII GAAGA 3673 3672 . . 3664 3667 + DpnI GATC 3665 3665 . . 3664 3667 + BstKTI GATC 3666 3664 . . 3664 3667 + MboI GATC 3663 3667 . . 3667 3672 + PvuII CAGCTG 3669 3669 . . 3667 3672 + NspBII* CMGCKG 3669 3669 . . 3668 3671 + SetI ASST 3671 3667 . . 3668 3671 + CviJI RGCY 3669 3669 . . 3668 3671 + AluI AGCT 3669 3669 . . 3677 3682 + PstI CTGCAG 3681 3677 . . 3677 3682 + SfeI* CTRYAG 3677 3681 . . 3678 3681 + CviRI* TGCA 3679 3679 . . 3689 3701 + BarI GAAGNNNNNNTAC 3681 3676 3713 3708 3692 3695 + Csp6I GTAC 3692 3694 . . 3692 3695 + RsaI GTAC 3693 3693 . . 3700 3704 + Tsp4CI* ACNGT 3702 3701 . . 3726 3734 + Tth111I GACNNNGTC 3729 3730 . . 3736 3741 + ApoI RAATTY 3736 3740 . . 3737 3740 + TspEI AATT 3736 3740 . . 3749 3752 + Csp6I GTAC 3749 3751 . . 3749 3752 + RsaI GTAC 3750 3750 . . 3769 3773 + MaeIII GTNAC 3768 3773 . . 3776 3779 + SetI ASST 3779 3775 . . 3788 3791 + MseI TTAA 3788 3790 . . 3793 3803 + BplI GAGNNNNNCTC 3784 3779 3816 3811 3795 3799 + FokI GGATG 3808 3812 . . 3795 3799 + BseGI GGATG 3801 3799 . . 3793 3796 - MnlI CCTC 3786 3785 . . 3799 3803 + HinfI GANTC 3799 3802 . . 3799 3803 - PleI GAGTC 3793 3794 . . 3799 3803 - MlyI GAGTC 3793 3793 . . 3793 3803 - BplI GAGNNNNNCTC 3816 3811 3784 3779 3803 3806 + AciI CCGC 3803 3805 . . 3802 3807 - EciI GGCGGA 3792 3790 . . 3819 3822 + SetI ASST 3822 3818 . . 3826 3830 + Hpy188I TCNGA 3828 3827 . . 3823 3827 - MboII GAAGA 3815 3814 . . 3824 3829 - Eco57MI CTGRAG 3809 3807 . . 3824 3829 - Eco57I CTGAAG 3809 3807 . . 3830 3834 + Tsp4CI* ACNGT 3832 3831 . . 3851 3855 - BsmAI GTCTC 3845 3849 . . 3865 3870 + SalI GTCGAC 3865 3869 . . 3865 3870 + Hpy166II GTNNAC 3867 3867 . . 3865 3870 + HindII GTYRAC 3867 3867 . . 3865 3870 + AccI GTMKAC 3866 3868 . . 3866 3869 + TaqI TCGA 3866 3868 . . 3871 3874 + SetI ASST 3874 3870 . . 3874 3879 + AhaIII* TTTAAA 3876 3876 . . 3875 3878 + MseI TTAA 3875 3877 . . 3879 3882 + TspEI AATT 3878 3882 . . 3883 3888 + BciVI GTATCC 3894 3893 . . 3887 3890 + AciI CCGC 3887 3889 . . 3890 3894 + BssKI CCNGG 3889 3894 . . 3890 3894 + BseBI CCWGG 3891 3892 . . 3890 3894 + ScrFI CCNGG 3891 3892 . . 3890 3894 + EcoRII CCWGG 3889 3894 . . 3891 3896 + Eco57MI CTGRAG 3912 3910 . . 3886 3891 - EciI GGCGGA 3876 3874 . . 3891 3896 + GsuI CTGGAG 3912 3910 . . 3898 3901 + CviJI RGCY 3899 3899 . . 3896 3899 - MnlI CCTC 3889 3888 . . 3903 3908 + TaqII GACCGA 3919 3917 . . 3921 3924 + SetI ASST 3924 3920 . . 3923 3926 + MaeI CTAG 3923 3925 . . 3925 3930 + XhoII RGATCY 3925 3929 . . 3926 3929 + DpnI GATC 3927 3927 . . 3926 3929 + BstKTI GATC 3928 3926 . . 3926 3929 + MboI GATC 3925 3929 . . 3926 3930 - BinI* GGATC 3920 3921 . . 3933 3936 + SetI ASST 3936 3932 . . 3935 3940 + Hpy166II GTNNAC 3937 3937 . . 3951 3955 + MaeIII GTNAC 3950 3955 . . 3949 3954 - TsoI TARCCA 3939 3937 . . 3964 3968 + BinI* GGATC 3972 3973 . . 3962 3965 - MnlI CCTC 3955 3954 . . 3965 3968 + DpnI GATC 3966 3966 . . 3965 3968 + BstKTI GATC 3967 3965 . . 3965 3968 + MboI GATC 3964 3968 . . 3972 3977 + Hpy166II GTNNAC 3974 3974 . . 3972 3977 + HpaI GTTAAC 3974 3974 . . 3972 3977 + HindII GTYRAC 3974 3974 . . 3973 3976 + MseI TTAA 3973 3975 . . 3979 3983 + BsrI ACTGG 3984 3982 . . 3982 3986 + HphI GGTGA 3994 3993 . . 3989 3992 + MseI TTAA 3989 3991 . . 3992 3995 + SetI ASST 3995 3991 . . 3999 4009 + BsiYI* CCNNNNNNNGG 4005 4002 . . 3998 4002 - TspGWI ACGGA 3988 3986 . . 4008 4012 + FokI GGATG 4021 4025 . . 4008 4012 + BseGI GGATG 4014 4012 . . 4010 4017 + FspAI RTGCGCAY 4013 4013 . . 4011 4016 + MstI* TGCGCA 4013 4013 . . 4012 4015 + Hin6I GCGC 4012 4014 . . 4012 4015 + GlaI GCGC 4013 4013 . . 4012 4015 + HhaI GCGC 4014 4012 . . 4009 4013 - SfaNI GCATC 3999 4003 . . 4024 4028 + Tsp4CI* ACNGT 4026 4025 . . 4033 4038 + Hpy166II GTNNAC 4035 4035 . . 4044 4047 + CviRI* TGCA 4045 4045 . . 4060 4063 + MseI TTAA 4060 4062 . . 4062 4065 + TspEI AATT 4061 4065 . . 4067 4071 - FokI GGATG 4053 4057 . . 4067 4071 - BseGI GGATG 4066 4064 . . 4074 4084 + Hin4I GAYNNNNNVTC 4065 4060 4097 4092 4075 4079 + BceAI ACGGC 4091 4093 . . 4077 4080 + CviJI RGCY 4078 4078 . . 4080 4085 + BclI TGATCA 4080 4084 . . 4081 4084 + DpnI GATC 4082 4082 . . 4081 4084 + BstKTI GATC 4083 4081 . . 4081 4084 + MboI GATC 4080 4084 . . 4074 4084 - Hin4I GAYNNNNNVTC 4097 4092 4065 4060 4085 4088 + SetI ASST 4088 4084 . . 4085 4088 + CviJI RGCY 4086 4086 . . 4085 4088 + AluI AGCT 4086 4086 . . 4086 4091 + Cac8I GCNNGC 4088 4088 . . 4086 4091 + BmtI GCTAGC 4090 4086 . . 4086 4091 + NheI GCTAGC 4086 4090 . . 4087 4090 + MaeI CTAG 4087 4089 . . 4092 4095 + SetI ASST 4095 4091 . . 4090 4095 - BspMI ACCTGC 4081 4085 . . 4100 4104 - MboII GAAGA 4092 4091 . . 4108 4118 + MwoI GCNNNNNNNGC 4114 4111 . . 4108 4118 + BstAPI GCANNNNNTGC 4114 4111 . . 4116 4119 + CviRI* TGCA 4117 4117 . . 4123 4126 + CviJI RGCY 4124 4124 . . 4123 4128 + NlaIV GGNNCC 4125 4125 . . 4120 4124 - BccI CCATC 4114 4115 . . 4129 4133 + DdeI CTNAG 4129 4132 . . 4129 4133 - BspCNI CTCAG 4121 4119 . . 4129 4133 - BseMII CTCAG 4120 4118 . . 4141 4144 + DpnI GATC 4142 4142 . . 4141 4144 + BstKTI GATC 4143 4141 . . 4141 4144 + MboI GATC 4140 4144 . . 4147 4152 + Esp3I CGTCTC 4153 4157 . . 4148 4152 + BsmAI GTCTC 4153 4157 . . 4149 4154 + Hpy178III* TCNNGA 4150 4152 . . 4145 4149 - BceAI ACGGC 4130 4132 . . 4151 4154 + TaqI TCGA 4151 4153 . . 4153 4156 + DpnI GATC 4154 4154 . . 4153 4156 + BstKTI GATC 4155 4153 . . 4153 4156 + MboI GATC 4152 4156 . . 4153 4157 - BinI* GGATC 4147 4148 . . 4162 4166 + TfiI GAWTC 4162 4165 . . 4162 4166 + HinfI GANTC 4162 4165 . . 4167 4170 + FatI CATG 4166 4170 . . 4167 4170 + NlaIII CATG 4170 4166 . . 4167 4170 + CviAII CATG 4167 4169 . . 4173 4176 + TaiI ACGT 4176 4172 . . 4173 4176 + SetI ASST 4176 4172 . . 4173 4176 + MaeII ACGT 4173 4175 . . 4174 4178 + Hpy99I CGWCG 4178 4173 . . 4179 4184 + Eco57MI CTGRAG 4200 4198 . . 4179 4184 + GsuI CTGGAG 4200 4198 . . 4185 4188 + SetI ASST 4188 4184 . . 4185 4188 + CviJI RGCY 4186 4186 . . 4185 4188 + AluI AGCT 4186 4186 . . 4214 4217 + MseI TTAA 4214 4216 . . 4223 4227 - TspDTI ATGAA 4213 4211 . . 4236 4239 + SetI ASST 4239 4235 . . 4235 4238 - MnlI CCTC 4228 4227 . . 4241 4245 + MaeIII GTNAC 4240 4245 . . 4239 4244 - TsoI TARCCA 4229 4227 . . 4250 4253 + TspEI AATT 4249 4253 . . 4257 4263 + TspRI CASTGNN 4263 4254 . . 4247 4257 - Hin4I GAYNNNNNVTC 4270 4265 4238 4233 4263 4266 + SetI ASST 4266 4262 . . 4263 4266 + CviJI RGCY 4264 4264 . . 4263 4266 + AluI AGCT 4264 4264 . . 4291 4301 + Hin4I GAYNNNNNVTC 4282 4277 4314 4309 4297 4300 + SetI ASST 4300 4296 . . 4298 4302 + BmgT120I GGNCC 4299 4300 . . 4298 4302 + AvaII GGWCC 4298 4301 . . 4298 4302 + AsuI* GGNCC 4298 4301 . . 4300 4304 + Hpy188I TCNGA 4302 4301 . . 4291 4301 - Hin4I GAYNNNNNVTC 4314 4309 4282 4277 4303 4307 + MboII GAAGA 4315 4314 . . 4305 4310 + XhoII RGATCY 4305 4309 . . 4306 4309 + DpnI GATC 4307 4307 . . 4306 4309 + BstKTI GATC 4308 4306 . . 4306 4309 + MboI GATC 4305 4309 . . 4308 4313 + BetI* WCCGGW 4308 4312 . . 4309 4312 + HpaII CCGG 4309 4311 . . 4306 4310 - BinI* GGATC 4300 4301 . . 4315 4319 + TfiI GAWTC 4315 4318 . . 4315 4319 + HinfI GANTC 4315 4318 . . 4326 4331 + BclI TGATCA 4326 4330 . . 4327 4330 + DpnI GATC 4328 4328 . . 4327 4330 + BstKTI GATC 4329 4327 . . 4327 4330 + MboI GATC 4326 4330 . . 4334 4337 + TaiI ACGT 4337 4333 . . 4334 4337 + SetI ASST 4337 4333 . . 4334 4337 + MaeII ACGT 4334 4336 . . 4336 4340 + MaeIII GTNAC 4335 4340 . . 4343 4347 + DdeI CTNAG 4343 4346 . . 4343 4347 + BspCNI CTCAG 4356 4354 . . 4343 4347 + BseMII CTCAG 4357 4355 . . 4347 4350 + Csp6I GTAC 4347 4349 . . 4347 4350 + RsaI GTAC 4348 4348 . . 4349 4353 + Tsp4CI* ACNGT 4351 4350 . . 4378 4381 + TaiI ACGT 4381 4377 . . 4378 4381 + SetI ASST 4381 4377 . . 4378 4381 + MaeII ACGT 4378 4380 . . 4381 4384 + CviRI* TGCA 4382 4382 . . 4382 4387 + NspI RCATGY 4386 4382 . . 4382 4387 + Cac8I GCNNGC 4384 4384 . . 4382 4387 + SphI GCATGC 4386 4382 . . 4377 4382 - BtrI CACGTC 4379 4379 . . 4383 4386 + FatI CATG 4382 4386 . . 4383 4386 + NlaIII CATG 4386 4382 . . 4383 4386 + CviAII CATG 4383 4385 . . 4385 4388 + CviRI* TGCA 4386 4386 . . 4388 4391 + SetI ASST 4391 4387 . . 4386 4391 - BspMI ACCTGC 4377 4381 . . 4392 4397 + Hpy178III* TCNNGA 4393 4395 . . 4392 4397 + BspHI TCATGA 4392 4396 . . 4393 4402 + MslI CAYNNNNRTG 4397 4397 . . 4393 4396 + FatI CATG 4392 4396 . . 4393 4396 + NlaIII CATG 4396 4392 . . 4393 4396 + CviAII CATG 4393 4395 . . 4391 4395 - TspDTI ATGAA 4381 4379 . . 4398 4403 + NspI RCATGY 4402 4398 . . 4398 4403 + Cac8I GCNNGC 4400 4400 . . 4398 4403 + SphI GCATGC 4402 4398 . . 4399 4402 + FatI CATG 4398 4402 . . 4399 4402 + NlaIII CATG 4402 4398 . . 4399 4402 + CviAII CATG 4399 4401 . . 4404 4408 + Hpy188I TCNGA 4406 4405 . . 4410 4413 + TspEI AATT 4409 4413 . . 4420 4424 + BslFI GGGAC 4434 4438 . . 4450 4455 + Hpy178III* TCNNGA 4451 4453 . . 4451 4456 + Eco57MI CTGRAG 4472 4470 . . 4451 4456 + GsuI CTGGAG 4472 4470 . . 4455 4460 + XhoII RGATCY 4455 4459 . . 4455 4460 + BglII AGATCT 4455 4459 . . 4456 4459 + DpnI GATC 4457 4457 . . 4456 4459 + BstKTI GATC 4458 4456 . . 4456 4459 + MboI GATC 4455 4459 . . 4464 4469 + EcoRI GAATTC 4464 4468 . . 4464 4469 + ApoI RAATTY 4464 4468 . . 4465 4468 + TspEI AATT 4464 4468 . . 4471 4476 + AhaIII* TTTAAA 4473 4473 . . 4472 4475 + MseI TTAA 4472 4474 . . 4483 4492 + MslI CAYNNNNRTG 4487 4487 . . 4495 4499 + MaeIII GTNAC 4494 4499 . . 4501 4506 + TatI WGTACW 4501 4505 . . 4502 4505 + Csp6I GTAC 4502 4504 . . 4502 4505 + RsaI GTAC 4503 4503 . . 4509 4512 + CviJI RGCY 4510 4510 . . 4510 4514 + TauI GCSGC 4513 4510 . . 4510 4514 + BlsI GCNGC 4512 4511 . . 4510 4514 + BisI GCNGC 4511 4512 . . 4511 4514 + AciI CCGC 4511 4513 . . 4520 4524 + TfiI GAWTC 4520 4523 . . 4520 4524 + HinfI GANTC 4520 4523 . . 4530 4534 + Tsp45I GTSAC 4529 4534 . . 4530 4534 + MaeIII GTNAC 4529 4534 . . 4532 4538 + TspRI CASTGNN 4538 4529 . . 4533 4537 + Tsp4CI* ACNGT 4535 4534 . . 4542 4547 + Ksp632I* CTCTTC 4548 4551 . . 4543 4547 - MboII GAAGA 4535 4534 . . 4552 4556 + Hpy188I TCNGA 4554 4553 . . 4556 4561 + EcoT22I ATGCAT 4560 4556 . . 4557 4560 + CviRI* TGCA 4558 4558 . . 4555 4559 - SfaNI GCATC 4545 4549 . . 4570 4573 + TaqI TCGA 4570 4572 . . 4567 4571 - MboII GAAGA 4559 4558 . . 4575 4578 + CviJI RGCY 4576 4576 . . 4572 4576 - BccI CCATC 4566 4567 . . 4581 4585 + BccI CCATC 4589 4590 . . 4585 4588 + MnlI CCTC 4595 4594 . . 4582 4586 - FokI GGATG 4568 4572 . . 4582 4586 - BseGI GGATG 4581 4579 . . 4603 4607 + TauI GCSGC 4606 4603 . . 4603 4607 + BlsI GCNGC 4605 4604 . . 4603 4607 + BisI GCNGC 4604 4605 . . 4604 4609 + CfrI YGGCCR 4604 4608 . . 4605 4608 + CviJI RGCY 4606 4606 . . 4605 4608 + HaeIII GGCC 4606 4606 . . 4603 4606 - AciI CCGC 4603 4605 . . 4612 4615 + TspEI AATT 4611 4615 . . 4617 4622 + Hpy178III* TCNNGA 4618 4620 . . 4621 4625 + TfiI GAWTC 4621 4624 . . 4621 4625 + HinfI GANTC 4621 4624 . . 4648 4652 + MboII GAAGA 4660 4659 . . 4648 4653 + BbvII* GAAGAC 4655 4659 . . 4659 4662 + TspEI AATT 4658 4662 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 53 338 808 1363 2112 # # AciI: # [CCGC] # 71 84 90 92 253 274 # 314 319 492 499 624 1562 # # AhaIII*: # [TTTAAA] # 201 259 597 3617 # # AluI: # [AGCT] # 8 78 100 127 135 138 # 147 162 166 177 258 299 # 306 319 329 410 417 443 # 655 # # AlwNI: # [CAGNNNCTG] # 1816 2858 # # ApaI: # [GGGCCC] # 1851 2823 # # ApoI: # [RAATTY] # 42 75 210 218 235 273 # 369 426 564 660 728 874 # # AsuI*: # [GGNCC] # 1 16 53 106 184 239 # 277 288 306 376 640 702 # 1486 # # AsuII: # [TTCGAA] # 944 1228 2502 # # AvaI: # [CYCGRG] # 1350 3324 # # AvaII: # [GGWCC] # 16 277 306 341 376 640 # 702 2016 # # BalI: # [TGGCCA] # 1462 3212 # # BamHI: # [GGATCC] # 447 4227 # # BarI: # [GAAGNNNNNNTAC] # 32 32 497 961 3152 # # BbvCI: # [CCTCAGC] # 20 4654 # # BbvI: # [GCAGC] # 3 3 3 12 102 116 # 157 263 787 1143 2085 # # BbvII*: # [GAAGAC] # 19 63 247 4345 # # BccI: # [CCATC] # 23 85 164 206 452 455 # 494 617 765 1413 # # Bce83I*: # [CTTGAG] # 2084 2590 # # BceAI: # [ACGGC] # 39 150 544 1032 2909 # # BcgI: # [CGANNNNNNTGC] # 34 1678 2962 # # BciVI: # [GTATCC] # 506 780 3388 # # BclI: # [TGATCA] # 246 348 4080 # # BdaI: # [TGANNNNNNTCA] # 0 0 0 0 34 34 # 999 1204 2403 # # BetI*: # [WCCGGW] # 228 366 603 922 2555 # # BfiI: # [ACTGGG] # 1026 3648 # # BglII: # [AGATCT] # 219 4455 # # BinI*: # [GGATC] # 13 52 153 162 175 280 # 374 764 997 1704 # # BisI: # [GCNGC] # 3 3 3 12 70 93 # 102 141 157 238 254 405 # 498 762 1933 # # BlsI: # [GCNGC] # 3 3 3 12 69 93 # 102 141 157 238 255 405 # 498 762 1933 # # BmeT110I: # [CYCGRG] # 1349 3325 # # BmgT120I: # [GGNCC] # 1 17 53 106 184 239 # 277 288 306 375 640 702 # 1486 # # BmtI: # [GCTAGC] # 584 4090 # # BplI: # [GAGNNNNNCTC] # 0 0 32 858 3784 # # Bpu10I: # [CCTNAGC] # 20 525 1107 3022 # # BsaAI: # [YACGTR] # 1229 1705 1740 # # BsaBI: # [GATNNNNATC] # 188 698 1800 1988 # # BsaXI: # [ACNNNNNCTCC] # 30 2146 2498 # # BseBI: # [CCWGG] # 59 338 355 667 783 2472 # # BseGI: # [GGATG] # 39 42 52 93 110 159 # 213 215 245 291 309 309 # 419 515 536 1127 # # BseMII: # [CTCAG] # 34 165 180 237 252 317 # 758 1122 1609 # # BseRI: # [GAGGAG] # 1319 3355 # # BseSI: # [GKGCMC] # 1851 2823 # # BseYI: # [CCCAGC] # 2125 2549 # # BsiYI*: # [CCNNNNNNNGG] # 376 669 1687 1942 # # BslFI: # [GGGAC] # 240 773 1129 2532 # # BsmAI: # [GTCTC] # 45 152 308 326 521 1439 # 1883 # # BsmI: # [GAATGC] # 1413 1514 1747 # # Bsp120I: # [GGGCCC] # 1847 2827 # # BspCNI: # [CTCAG] # 33 165 178 235 254 318 # 758 1122 1611 # # BspHI: # [TCATGA] # 282 741 3651 # # BspMI: # [ACCTGC] # 135 296 297 629 994 2323 # # BspMII*: # [TCCGGA] # 831 1288 2555 # # BsrI: # [ACTGG] # 124 330 579 690 732 2219 # # BssKI: # [CCNGG] # 59 338 353 667 785 2472 # # BssNAI: # [GTATAC] # 1702 2972 # # BstAPI: # [GCANNNNNTGC] # 560 4114 # # BstKTI: # [GATC] # 12 21 39 54 60 90 # 116 129 153 162 196 216 # 234 247 262 539 777 1367 # # BstXI: # [CCANNNNNNTGG] # 1433 3241 # # BtgZI: # [GCGATG] # 2000 2674 # # BtrI: # [CACGTC] # 295 4379 # # BtsI: # [GCAGTG] # 1170 1463 2041 # # Cac8I: # [GCNNGC] # 16 274 296 2029 2059 # # CfrI: # [YGGCCR] # 70 1460 3144 # # ClaI: # [ATCGAT] # 1094 3580 # # Csp6I: # [GTAC] # 57 91 155 172 178 219 # 242 250 405 447 548 598 # 1312 # # CviAII: # [CATG] # 6 10 24 44 55 98 # 99 109 143 161 194 203 # 216 275 277 573 983 1204 # # CviJI: # [RGCY] # 5 6 8 8 8 9 # 10 22 24 24 29 30 # 30 36 38 41 47 52 # 53 62 66 68 70 78 # 83 99 105 106 106 109 # 114 115 127 130 162 171 # 179 187 223 230 246 252 # 282 299 525 # # CviRI*: # [TGCA] # 4 20 33 41 43 71 # 72 95 103 116 138 145 # 159 168 172 190 265 319 # 360 366 396 400 479 519 # # DdeI: # [CTNAG] # 13 14 20 45 56 157 # 165 214 252 275 331 467 # 758 799 1108 # # DpnI: # [GATC] # 12 21 39 54 60 90 # 116 129 153 162 196 217 # 233 247 262 539 777 1367 # # DraII: # [RGGNCCY] # 946 1355 2373 # # DsaI*: # [CCRYGG] # 85 461 1198 2930 # # EciI: # [GGCGGA] # 84 293 798 3499 # # Eco57I: # [CTGAAG] # 185 567 865 1404 1653 # # Eco57MI: # [CTGRAG] # 103 185 202 272 288 567 # 1404 1653 # # EcoNI: # [CCTNNNNNAGG] # 2316 2358 # # EcoP15I: # [CAGCAG] # 3 3 3 15 1005 1579 # 2066 # # EcoRI: # [GAATTC] # 210 4464 # # EcoRII: # [CCWGG] # 59 338 353 667 785 2472 # # EcoRV: # [GATATC] # 359 1477 2838 # # EcoT22I: # [ATGCAT] # 114 1398 3162 # # Esp3I: # [CGTCTC] # 521 634 3519 # # EspI*: # [GCTNAGC] # 1974 2700 # # FalI: # [AAGNNNNNCTT] # 0 0 0 0 32 32 # 480 1703 2427 # # FatI: # [CATG] # 6 10 24 44 54 98 # 99 109 143 161 194 203 # 216 276 277 573 983 1204 # # FauI: # [CCCGC] # 314 492 849 3019 # # FnuDII*: # [CGCG] # 103 651 3920 # # FokI: # [GGATG] # 32 42 59 106 110 179 # 213 215 245 296 309 311 # 399 515 516 1127 # # FspAI: # [RTGCGCAY] # 661 4013 # # GlaI: # [GCGC] # 114 405 641 661 1019 1834 # # GsaI: # [CCCAGC] # 2121 2553 # # GsuI: # [CTGGAG] # 202 272 288 3912 # # HaeII: # [RGCGCY] # 1678 2996 # # HaeIII: # [GGCC] # 68 107 238 256 280 1206 # 2519 # # HgiJII*: # [GRGCYC] # 1851 2823 # # HhaI: # [GCGC] # 114 405 642 660 1019 1834 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 0 0 # 0 0 0 0 12 32 # 32 32 32 32 32 44 # 141 360 456 771 2698 # # Hin4II*: # [CCTTC] # 2310 2364 # # Hin6I: # [GCGC] # 114 405 640 662 1019 1834 # # HindII: # [GTYRAC] # 107 217 236 700 1411 2003 # # HindIII: # [AAGCTT] # 441 4233 # # HinfI: # [GANTC] # 8 9 12 16 53 94 # 101 110 153 156 159 159 # 199 205 321 363 416 428 # 470 1242 # # HpaI: # [GTTAAC] # 700 3974 # # HpaII: # [CCGG] # 4 228 365 595 600 922 # 1960 # # HphI: # [GGTGA] # 57 70 83 150 178 517 # 680 869 2070 # # Hpy166II: # [GTNNAC] # 37 53 61 70 217 236 # 248 339 618 639 793 1363 # # Hpy178III*: # [TCNNGA] # 8 36 54 56 58 60 # 61 70 102 132 153 167 # 216 220 243 318 321 344 # 387 396 579 693 # # Hpy188I: # [TCNGA] # 30 36 56 95 103 104 # 112 120 148 158 225 287 # 411 474 540 558 608 609 # # Hpy99I: # [CGWCG] # 496 4178 # # Ksp632I*: # [CTCTTC] # 59 102 126 564 3823 # # MaeI: # [CTAG] # 102 164 186 303 351 408 # 587 595 798 1180 # # MaeII: # [ACGT] # 44 83 84 161 296 645 # 694 1010 1657 # # MaeIII: # [GTNAC] # 35 79 95 145 159 160 # 182 239 267 290 498 829 # 844 852 # # MboI: # [GATC] # 12 21 39 54 60 90 # 116 129 153 162 196 219 # 231 247 262 539 777 1367 # # MboII: # [GAAGA] # 14 14 21 24 53 59 # 60 63 76 98 101 102 # 117 128 142 145 191 220 # 223 225 235 260 277 333 # 399 504 590 # # MfeI: # [CAATTG] # 115 1091 1265 2203 # # MlyI: # [GAGTC] # 13 110 352 424 477 485 # 522 881 1410 # # MmeI: # [TCCRAC] # 684 1013 1089 1888 # # MnlI: # [CCTC] # 15 19 29 66 79 81 # 103 124 130 133 141 181 # 203 223 247 273 291 310 # 312 367 495 852 # # MseI: # [TTAA] # 16 28 34 42 58 71 # 87 97 98 154 172 197 # 198 202 231 258 291 321 # 332 342 365 423 657 # # MslI: # [CAYNNNNRTG] # 90 187 314 1800 2283 # # MstI*: # [TGCGCA] # 661 4013 # # MwoI: # [GCNNNNNNNGC] # 12 30 51 323 560 609 # 852 934 1303 # # NcoI: # [CCATGG] # 1283 3391 # # NheI: # [GCTAGC] # 588 4086 # # NlaIII: # [CATG] # 6 10 24 44 58 98 # 99 109 143 161 194 203 # 216 272 277 573 983 1204 # # NlaIV: # [GGNNCC] # 1 147 379 437 449 508 # 549 804 1400 # # NruI: # [TCGCGA] # 103 4571 # # NspBII*: # [CMGCKG] # 299 1005 3370 # # NspI: # [RCATGY] # 16 272 1959 2427 # # PfoI: # [TCCNGGA] # 353 1123 3198 # # PleI: # [GAGTC] # 14 110 352 424 477 484 # 522 881 1410 # # PmaCI: # [CACGTG] # 1229 3445 # # PpuMI: # [RGGWCCY] # 946 1355 2373 # # PstI: # [CTGCAG] # 993 3681 # # PvuII: # [CAGCTG] # 299 1005 3370 # # RsaI: # [GTAC] # 57 91 155 171 178 219 # 242 250 406 447 548 598 # 1312 # # SalI: # [GTCGAC] # 809 3865 # # SanDI: # [GGGWCCC] # 1355 3319 # # SapI: # [GCTCTTC] # 59 4615 # # ScaI: # [AGTACT] # 341 954 3379 # # ScrFI: # [CCNGG] # 59 338 355 667 783 2472 # # SduI: # [GDGCHC] # 1851 2823 # # SecI*: # [CCNNGG] # 85 178 461 1020 1351 1579 # # SetI: # [ASST] # 7 8 9 10 12 12 # 14 15 16 16 18 18 # 19 21 24 27 27 28 # 31 34 35 36 37 38 # 43 44 48 50 50 51 # 52 53 59 62 62 67 # 75 81 85 89 93 97 # 108 119 120 124 127 135 # 135 140 142 150 163 177 # 247 261 269 283 301 # # SfaNI: # [GCATC] # 54 90 129 147 260 319 # 546 808 896 1425 # # SfeI*: # [CTRYAG] # 312 997 3365 # # SmlI: # [CTYRAG] # 2105 2569 # # SpeI: # [ACTAGT] # 1941 2733 # # SphI: # [GCATGC] # 16 272 4386 # # SspI: # [AATATT] # 94 1003 1223 2354 # # StyI: # [CCWWGG] # 1283 3391 # # TaiI: # [ACGT] # 44 83 84 161 293 645 # 694 1013 1657 # # TaqI: # [TCGA] # 42 50 77 104 208 234 # 237 285 286 288 419 461 # 583 675 725 # # TaqII: # [GACCGA] # 755 3919 # # TatI: # [WGTACW] # 173 178 341 952 3030 # # TauI: # [GCSGC] # 68 93 256 498 3759 # # TfiI: # [GAWTC] # 53 101 130 153 159 205 # 315 337 437 763 779 1242 # # TseI: # [GCWGC] # 3 3 3 12 102 141 # 157 238 762 1156 2097 # # TsoI: # [TARCCA] # 33 66 290 445 576 621 # 2643 # # Tsp45I: # [GTSAC] # 79 145 829 852 1267 1502 # # Tsp4CI*: # [ACNGT] # 45 63 69 130 139 158 # 184 194 197 201 213 298 # 325 344 352 804 958 # # TspDTI: # [ATGAA] # 19 51 65 75 78 84 # 102 103 104 139 168 174 # 187 203 203 293 405 427 # 477 555 762 # # TspEI: # [AATT] # 11 12 13 16 16 16 # 17 20 21 23 25 29 # 30 32 36 36 38 41 # 42 47 48 48 55 75 # 79 81 85 127 132 142 # 145 147 160 165 183 186 # 188 206 224 233 273 276 # 281 306 308 # # TspGWI: # [ACGGA] # 219 335 545 686 1275 1614 # # TspRI: # [CASTGNN] # 136 275 344 468 603 626 # 675 759 788 # # TstI: # [CACNNNNNNTCC] # 32 524 4118 # # Tth111I: # [GACNNNGTC] # 945 3729 # # VspI: # [ATTAAT] # 685 1206 2783 # # XcmI: # [CCANNNNNNNNNTGG] # 1670 3004 # # XhoII: # [RGATCY] # 150 219 380 447 997 2481 # # XmnI: # [GAANNNNTTC] # 22 109 386 434 493 599 # 1222 1409 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 4674 # Reported_sequences: 1 # Reported_hitcount: 1095 #---------------------------------------