Restriction Fragments for YHR137C-A



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The currently selected gene/sequence is :

YHR137C-A on chromosome VIII from coordinates 376388 to 375738.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.21392.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 10 16 + PpuMI RGGWCCY 11 14 . . 10 16 + DraII RGGNCCY 11 14 . . 11 16 + NlaIV GGNNCC 13 13 . . 11 15 + BmgT120I GGNCC 12 13 . . 11 15 + AvaII GGWCC 11 14 . . 11 15 + AsuI* GGNCC 11 14 . . 15 20 + StyI CCWWGG 15 19 . . 15 20 + SecI* CCNNGG 15 19 . . 19 22 + CviJI RGCY 20 20 . . 26 29 + MseI TTAA 26 28 . . 40 44 + BmgT120I GGNCC 41 42 . . 40 44 + AvaII GGWCC 40 43 . . 40 44 + AsuI* GGNCC 40 43 . . 46 51 + Hpy166II GTNNAC 48 48 . . 46 51 + HindII GTYRAC 48 48 . . 43 47 - BsrI ACTGG 43 41 . . 49 59 + Hin4I GAYNNNNNVTC 40 35 72 67 53 56 + TspEI AATT 52 56 . . 58 61 + TaqI TCGA 58 60 . . 49 59 - Hin4I GAYNNNNNVTC 72 67 40 35 79 82 + TaqI TCGA 79 81 . . 91 97 + PasI CCCWGGG 92 95 . . 91 96 + SecI* CCNNGG 91 95 . . 92 96 + BssKI CCNGG 91 96 . . 92 96 + BseBI CCWGG 93 94 . . 92 97 + SecI* CCNNGG 92 96 . . 92 96 + ScrFI CCNGG 93 94 . . 92 96 + EcoRII CCWGG 91 96 . . 95 99 + BslFI GGGAC 109 113 . . 97 101 + HinfI GANTC 97 100 . . 97 107 + Hin4I GAYNNNNNVTC 88 83 120 115 97 107 + Eam1105I GACNNNNNGTC 102 101 . . 97 101 - PleI GAGTC 91 92 . . 97 101 - MlyI GAGTC 91 91 . . 106 109 + TaqI TCGA 106 108 . . 97 107 - Hin4I GAYNNNNNVTC 120 115 88 83 110 113 + SetI ASST 113 109 . . 108 112 - Hin4II* CCTTC 102 101 . . 115 118 + SetI ASST 118 114 . . 120 129 + MslI CAYNNNNRTG 124 124 . . 118 122 - TspDTI ATGAA 108 106 . . 122 127 + Hpy178III* TCNNGA 123 125 . . 126 130 - BccI CCATC 120 121 . . 131 135 + FokI GGATG 144 148 . . 131 135 + BseGI GGATG 137 135 . . 135 145 + Hin4I GAYNNNNNVTC 126 121 158 153 136 146 + BsaXI ACNNNNNCTCC 126 123 156 153 142 152 + MwoI GCNNNNNNNGC 148 145 . . 146 151 + SfeI* CTRYAG 146 150 . . 150 153 + CviJI RGCY 151 151 . . 152 155 + MnlI CCTC 162 161 . . 168 171 + CviJI RGCY 169 169 . . 170 174 + BssKI CCNGG 169 174 . . 170 173 + HpaII CCGG 170 172 . . 170 175 + SecI* CCNNGG 170 174 . . 170 174 + ScrFI CCNGG 171 172 . . 170 174 + CauII* CCSGG 171 172 . . 174 178 + HphI GGTGA 186 185 . . 181 186 + Hpy166II GTNNAC 183 183 . . 186 191 + Ksp632I* CTCTTC 192 195 . . 187 191 - MboII GAAGA 179 178 . . 195 204 + MslI CAYNNNNRTG 199 199 . . 199 203 + Tsp4CI* ACNGT 201 200 . . 200 206 + TspRI CASTGNN 206 197 . . 206 209 + TaqI TCGA 206 208 . . 208 212 + TfiI GAWTC 208 211 . . 208 212 + HinfI GANTC 208 211 . . 212 216 + Hpy99I CGWCG 216 211 . . 219 222 + TspEI AATT 218 222 . . 232 235 + SetI ASST 235 231 . . 235 238 + MseI TTAA 235 237 . . 244 248 + TfiI GAWTC 244 247 . . 244 248 + HinfI GANTC 244 247 . . 252 257 + NlaIV GGNNCC 254 254 . . 252 256 + BmgT120I GGNCC 253 254 . . 252 256 + AvaII GGWCC 252 255 . . 252 256 + AsuI* GGNCC 252 255 . . 249 254 - MmeI TCCRAC 230 228 . . 255 259 + FauI CCCGC 263 265 . . 256 259 + AciI CCGC 256 258 . . 266 270 + FokI GGATG 279 283 . . 266 270 + BseGI GGATG 272 270 . . 272 276 - FokI GGATG 258 262 . . 272 276 - BseGI GGATG 271 269 . . 276 280 - BsrI ACTGG 276 274 . . 275 280 - BfiI ACTGGG 270 269 . . 283 287 + HinfI GANTC 283 286 . . 283 293 + Hin4I GAYNNNNNVTC 274 269 306 301 283 291 + Tth111I GACNNNGTC 286 287 . . 283 287 - PleI GAGTC 277 278 . . 283 287 - MlyI GAGTC 277 277 . . 288 293 + Esp3I CGTCTC 294 298 . . 289 293 + BsmAI GTCTC 294 298 . . 286 290 - TspGWI ACGGA 276 274 . . 295 299 + Hpy188I TCNGA 297 296 . . 303 306 + CviJI RGCY 304 304 . . 304 309 - GsaI CCCAGC 308 304 . . 304 309 - BseYI CCCAGC 304 308 . . 312 317 + VspI ATTAAT 313 315 . . 313 316 + MseI TTAA 313 315 . . 315 318 + TspEI AATT 314 318 . . 318 323 + Hpy178III* TCNNGA 319 321 . . 322 332 + Hin4I GAYNNNNNVTC 313 308 345 340 322 332 - Hin4I GAYNNNNNVTC 345 340 313 308 334 337 + MseI TTAA 334 336 . . 350 353 - MnlI CCTC 343 342 . . 356 361 + Hpy166II GTNNAC 358 358 . . 362 367 + StyI CCWWGG 362 366 . . 362 367 + SecI* CCNNGG 362 366 . . 372 375 + CviRI* TGCA 373 373 . . 387 390 + TaiI ACGT 390 386 . . 387 390 + SetI ASST 390 386 . . 387 390 + MaeII ACGT 387 389 . . 390 393 + CviRI* TGCA 391 391 . . 380 391 - TstI CACNNNNNNTCC 404 399 372 367 391 396 + BsrDI GCAATG 398 396 . . 400 403 + SetI ASST 403 399 . . 400 403 + CviJI RGCY 401 401 . . 400 403 + AluI AGCT 401 401 . . 404 410 + TspRI CASTGNN 410 401 . . 405 409 + BsrI ACTGG 410 408 . . 405 410 + BfiI ACTGGG 415 414 . . 411 416 + XhoII RGATCY 411 415 . . 411 416 + NlaIV GGNNCC 413 413 . . 411 416 + BamHI GGATCC 411 415 . . 411 415 + BinI* GGATC 419 420 . . 412 422 + Hin4I GAYNNNNNVTC 403 398 435 430 412 415 + DpnI GATC 413 413 . . 412 415 + BstKTI GATC 414 412 . . 412 415 + MboI GATC 411 415 . . 412 416 - BinI* GGATC 406 407 . . 422 436 + XcmI CCANNNNNNNNNTGG 429 428 . . 412 422 - Hin4I GAYNNNNNVTC 435 430 403 398 428 431 + Hin6I GCGC 428 430 . . 428 431 + GlaI GCGC 429 429 . . 428 431 + HhaI GCGC 430 428 . . 431 436 + DsaI* CCRYGG 431 435 . . 431 436 + SecI* CCNNGG 431 435 . . 430 434 - BceAI ACGGC 415 417 . . 436 441 + Hpy166II GTNNAC 438 438 . . 443 446 + TspEI AATT 442 446 . . 466 469 + MseI TTAA 466 468 . . 499 503 + TfiI GAWTC 499 502 . . 499 503 + HinfI GANTC 499 502 . . 502 505 + TaqI TCGA 502 504 . . 527 532 - BsmI GAATGC 527 525 . . 537 540 + SetI ASST 540 536 . . 537 540 + CviJI RGCY 538 538 . . 537 540 + AluI AGCT 538 538 . . 538 543 + Cac8I GCNNGC 540 540 . . 538 543 + BmtI GCTAGC 542 538 . . 538 543 + NheI GCTAGC 538 542 . . 539 542 + MaeI CTAG 539 541 . . 541 544 + SetI ASST 544 540 . . 541 544 + CviJI RGCY 542 542 . . 541 544 + AluI AGCT 542 542 . . 549 554 + ZraI GACGTC 551 551 . . 549 554 + AcyI GRCGYC 550 552 . . 549 554 + AatII GACGTC 553 549 . . 550 553 + TaiI ACGT 553 549 . . 550 553 + SetI ASST 553 549 . . 550 553 + MaeII ACGT 550 552 . . 556 560 + Hpy188I TCNGA 558 557 . . 557 561 + DdeI CTNAG 557 560 . . 561 566 + EcoRV GATATC 563 563 . . 557 561 - BspCNI CTCAG 549 547 . . 557 561 - BseMII CTCAG 548 546 . . 565 569 + Hpy188I TCNGA 567 566 . . 571 575 + SfaNI GCATC 580 584 . . 572 576 - FokI GGATG 558 562 . . 572 576 - BseGI GGATG 571 569 . . 580 585 + HindIII AAGCTT 580 584 . . 581 584 + SetI ASST 584 580 . . 581 584 + CviJI RGCY 582 582 . . 581 584 + AluI AGCT 582 582 . . 587 591 + Hpy188I TCNGA 589 588 . . 591 596 + XhoII RGATCY 591 595 . . 591 596 + BglII AGATCT 591 595 . . 592 595 + DpnI GATC 593 593 . . 592 595 + BstKTI GATC 594 592 . . 592 595 + MboI GATC 591 595 . . 605 610 + Ksp632I* CTCTTC 611 614 . . 609 614 + Hpy178III* TCNNGA 610 612 . . 606 610 - MboII GAAGA 598 597 . . 610 615 + Eco57MI CTGRAG 631 629 . . 610 615 + GsuI CTGGAG 631 629 . . 620 624 + Tsp4CI* ACNGT 622 621 . . 627 636 + XmnI GAANNNNTTC 631 631 . . 623 628 - MmeI TCCRAC 604 602 . . 628 638 + FalI AAGNNNNNCTT 619 614 651 646 632 636 - MboII GAAGA 624 623 . . 638 641 + MseI TTAA 638 640 . . #--------------------------------------- # # # AatII: # [GACGTC] # 98 553 # # AciI: # [CCGC] # 256 395 # # AcyI: # [GRCGYC] # 101 550 # # AluI: # [AGCT] # 4 40 69 137 401 # # AsuI*: # [GGNCC] # 11 29 212 399 # # AvaII: # [GGWCC] # 11 29 212 399 # # BamHI: # [GGATCC] # 240 411 # # BccI: # [CCATC] # 120 531 # # BceAI: # [ACGGC] # 236 415 # # BfiI: # [ACTGGG] # 145 236 270 # # BglII: # [AGATCT] # 60 591 # # BinI*: # [GGATC] # 13 232 406 # # BmgT120I: # [GGNCC] # 12 29 212 398 # # BmtI: # [GCTAGC] # 109 542 # # BsaXI: # [ACNNNNNCTCC] # 30 126 495 # # BseBI: # [CCWGG] # 93 558 # # BseGI: # [GGATG] # 1 80 134 137 299 # # BseMII: # [CTCAG] # 103 548 # # BseYI: # [CCCAGC] # 304 347 # # BslFI: # [GGGAC] # 109 542 # # BsmAI: # [GTCTC] # 294 357 # # BsmI: # [GAATGC] # 124 527 # # BspCNI: # [CTCAG] # 102 549 # # BsrDI: # [GCAATG] # 253 398 # # BsrI: # [ACTGG] # 43 134 233 241 # # BssKI: # [CCNGG] # 78 91 482 # # BstKTI: # [GATC] # 57 180 414 # # Cac8I: # [GCNNGC] # 111 540 # # CauII*: # [CCSGG] # 171 480 # # CviJI: # [RGCY] # 4 18 20 40 69 97 # 131 135 137 # # CviRI*: # [TGCA] # 18 260 373 # # DdeI: # [CTNAG] # 94 557 # # DpnI: # [GATC] # 58 180 413 # # DraII: # [RGGNCCY] # 11 640 # # DsaI*: # [CCRYGG] # 220 431 # # Eam1105I: # [GACNNNNNGTC] # 102 549 # # Eco57MI: # [CTGRAG] # 20 631 # # EcoRII: # [CCWGG] # 91 560 # # EcoRV: # [GATATC] # 88 563 # # Esp3I: # [CGTCTC] # 294 357 # # FalI: # [AAGNNNNNCTT] # 0 32 619 # # FauI: # [CCCGC] # 263 388 # # FokI: # [GGATG] # 21 93 114 144 279 # # GlaI: # [GCGC] # 222 429 # # GsaI: # [CCCAGC] # 308 343 # # GsuI: # [CTGGAG] # 20 631 # # HhaI: # [GCGC] # 221 430 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 0 0 # 0 0 6 7 16 32 # 32 32 32 32 32 40 # 58 116 216 # # Hin4II*: # [CCTTC] # 102 549 # # Hin6I: # [GCGC] # 223 428 # # HindII: # [GTYRAC] # 48 603 # # HindIII: # [AAGCTT] # 71 580 # # HinfI: # [GANTC] # 36 39 97 111 152 216 # # HpaII: # [CCGG] # 170 481 # # HphI: # [GGTGA] # 186 465 # # Hpy166II: # [GTNNAC] # 48 80 135 175 213 # # Hpy178III*: # [TCNNGA] # 41 123 196 291 # # Hpy188I: # [TCNGA] # 9 22 62 261 297 # # Hpy99I: # [CGWCG] # 216 435 # # Ksp632I*: # [CTCTTC] # 40 192 419 # # MaeI: # [CTAG] # 112 539 # # MaeII: # [ACGT] # 101 163 387 # # MboI: # [GATC] # 60 180 411 # # MboII: # [GAAGA] # 26 27 179 419 # # MlyI: # [GAGTC] # 91 186 374 # # MmeI: # [TCCRAC] # 47 230 374 # # MnlI: # [CCTC] # 162 181 308 # # MseI: # [TTAA] # 13 21 26 78 132 172 # 209 # # MslI: # [CAYNNNNRTG] # 75 124 452 # # MwoI: # [GCNNNNNNNGC] # 148 503 # # NheI: # [GCTAGC] # 113 538 # # NlaIV: # [GGNNCC] # 13 159 238 241 # # PasI: # [CCCWGGG] # 92 559 # # PleI: # [GAGTC] # 91 186 374 # # PpuMI: # [RGGWCCY] # 11 640 # # ScrFI: # [CCNGG] # 78 93 480 # # SecI*: # [CCNNGG] # 1 15 69 76 78 192 # 220 # # SetI: # [ASST] # 4 5 9 13 31 67 # 113 117 137 155 # # SfaNI: # [GCATC] # 71 580 # # SfeI*: # [CTRYAG] # 146 505 # # StyI: # [CCWWGG] # 15 289 347 # # TaiI: # [ACGT] # 98 163 390 # # TaqI: # [TCGA] # 21 27 58 100 149 296 # # TfiI: # [GAWTC] # 36 152 208 255 # # Tsp4CI*: # [ACNGT] # 29 201 421 # # TspDTI: # [ATGAA] # 108 543 # # TspEI: # [AATT] # 52 96 128 166 209 # # TspGWI: # [ACGGA] # 276 375 # # TspRI: # [CASTGNN] # 204 206 241 # # TstI: # [CACNNNNNNTCC] # 32 247 372 # # Tth111I: # [GACNNNGTC] # 286 365 # # VspI: # [ATTAAT] # 313 338 # # XcmI: # [CCANNNNNNNNNTGG] # 222 429 # # XhoII: # [RGATCY] # 60 180 411 # # XmnI: # [GAANNNNTTC] # 20 631 # # ZraI: # [GACGTC] # 100 551 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 651 # Reported_sequences: 1 # Reported_hitcount: 192 #---------------------------------------