Restriction Fragments for SPL2/YHR136C



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The currently selected gene/sequence is :

SPL2/YHR136C on chromosome VIII from coordinates 375100 to 374654.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.4865.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 3 8 + SduI GDGCHC 7 3 . . 3 8 + BseSI GKGCMC 7 3 . . 18 23 + VspI ATTAAT 19 21 . . 19 22 + MseI TTAA 19 21 . . 37 42 + AclI AACGTT 38 40 . . 38 41 + TaiI ACGT 41 37 . . 38 41 + SetI ASST 41 37 . . 38 41 + MaeII ACGT 38 40 . . 41 45 - TspDTI ATGAA 31 29 . . 64 67 + CviJI RGCY 65 65 . . 66 80 + XcmI CCANNNNNNNNNTGG 73 72 . . 66 70 + BssKI CCNGG 65 70 . . 66 70 + BseBI CCWGG 67 68 . . 66 71 + SecI* CCNNGG 66 70 . . 66 70 + ScrFI CCNGG 67 68 . . 66 70 + EcoRII CCWGG 65 70 . . 75 78 + TspEI AATT 74 78 . . 80 89 + PshAI GACNNNNGTC 84 84 . . 87 91 + BsmAI GTCTC 92 96 . . 94 97 + FnuDII* CGCG 95 95 . . 95 99 + TauI GCSGC 98 95 . . 95 99 + BlsI GCNGC 97 96 . . 95 99 + BisI GCNGC 96 97 . . 95 98 - AciI CCGC 95 97 . . 110 115 + EcoP15I CAGCAG 140 142 . . 140 143 + MseI TTAA 140 142 . . 148 154 + BstEII GGTNACC 148 153 . . 149 153 + Tsp45I GTSAC 148 153 . . 149 153 + MaeIII GTNAC 148 153 . . 145 149 - BccI CCATC 139 140 . . 153 163 + BsiYI* CCNNNNNNNGG 159 156 . . 150 154 - HphI GGTGA 142 141 . . 164 168 + MboII GAAGA 176 175 . . 175 179 + Hpy188I TCNGA 177 176 . . 176 181 + SecI* CCNNGG 176 180 . . 178 183 + BseRI GAGGAG 193 191 . . 178 181 - MnlI CCTC 171 170 . . 183 193 + Hin4I GAYNNNNNVTC 174 169 206 201 183 186 + DpnI GATC 184 184 . . 183 186 + BstKTI GATC 185 183 . . 183 186 + MboI GATC 182 186 . . 200 203 + CviJI RGCY 201 201 . . 198 201 - MnlI CCTC 191 190 . . 210 215 + BsgI GTGCAG 231 229 . . 211 214 + CviRI* TGCA 212 212 . . 218 221 + CviJI RGCY 219 219 . . 216 219 - MnlI CCTC 209 208 . . 216 226 - Hin4I GAYNNNNNVTC 239 234 207 202 230 233 + MaeI CTAG 230 232 . . 233 237 + MaeIII GTNAC 232 237 . . 245 250 + BsaAI YACGTR 247 247 . . 246 249 + TaiI ACGT 249 245 . . 246 249 + SetI ASST 249 245 . . 246 249 + MaeII ACGT 246 248 . . 254 259 - BtgZI GCGATG 239 243 . . 261 265 + Tsp45I GTSAC 260 265 . . 261 265 + MaeIII GTNAC 260 265 . . 271 275 + DdeI CTNAG 271 274 . . 271 275 + BspCNI CTCAG 284 282 . . 271 275 + BseMII CTCAG 285 283 . . 273 279 + TspRI CASTGNN 279 270 . . 277 281 + BinI* GGATC 285 286 . . 278 281 + DpnI GATC 279 279 . . 278 281 + BstKTI GATC 280 278 . . 278 281 + MboI GATC 277 281 . . 286 291 + AhaIII* TTTAAA 288 288 . . 287 290 + MseI TTAA 287 289 . . 302 307 + TatI WGTACW 302 306 . . 303 306 + Csp6I GTAC 303 305 . . 303 306 + RsaI GTAC 304 304 . . 307 312 + VspI ATTAAT 308 310 . . 308 311 + MseI TTAA 308 310 . . 319 323 + TfiI GAWTC 319 322 . . 319 323 + HinfI GANTC 319 322 . . 331 334 + Hin6I GCGC 331 333 . . 331 334 + GlaI GCGC 332 332 . . 331 334 + HhaI GCGC 333 331 . . 333 337 + BlsI GCNGC 335 334 . . 333 337 + BisI GCNGC 334 335 . . 333 337 + BbvI GCAGC 345 349 . . 333 337 + TseI GCWGC 333 336 . . 336 340 - HgaI GACGC 325 330 . . 346 350 + BlsI GCNGC 348 347 . . 346 350 + BisI GCNGC 347 348 . . 346 350 + TseI GCWGC 346 349 . . 349 353 + TauI GCSGC 352 349 . . 349 353 + BlsI GCNGC 351 350 . . 349 353 + BisI GCNGC 350 351 . . 346 350 - BbvI GCAGC 333 337 . . 350 353 + AciI CCGC 350 352 . . 352 356 - BceAI ACGGC 337 339 . . 358 361 + MseI TTAA 358 360 . . 362 367 + Cac8I GCNNGC 364 364 . . 365 368 + SetI ASST 368 364 . . 365 368 + CviJI RGCY 366 366 . . 365 368 + AluI AGCT 366 366 . . 366 371 + Cac8I GCNNGC 368 368 . . 373 376 + FatI CATG 372 376 . . 373 376 + NlaIII CATG 376 372 . . 373 376 + CviAII CATG 373 375 . . 391 399 + Tth111I GACNNNGTC 394 395 . . 392 396 + Tsp4CI* ACNGT 394 393 . . 406 410 + MboII GAAGA 418 417 . . 406 411 + BbvII* GAAGAC 413 417 . . 415 420 + AsuII TTCGAA 416 418 . . 416 419 + TaqI TCGA 416 418 . . 418 422 + MboII GAAGA 430 429 . . 421 425 + TfiI GAWTC 421 424 . . 421 425 + HinfI GANTC 421 424 . . 427 431 + Hpy188I TCNGA 429 428 . . 433 437 + MboII GAAGA 445 444 . . 442 447 + Hpy178III* TCNNGA 443 445 . . 442 447 + BspHI TCATGA 442 446 . . 443 446 + FatI CATG 442 446 . . 443 446 + NlaIII CATG 446 442 . . 443 446 + CviAII CATG 443 445 . . #--------------------------------------- # # # AciI: # [CCGC] # 95 97 255 # # AclI: # [AACGTT] # 38 409 # # AhaIII*: # [TTTAAA] # 159 288 # # AluI: # [AGCT] # 81 366 # # AsuII: # [TTCGAA] # 31 416 # # BbvI: # [GCAGC] # 12 102 333 # # BbvII*: # [GAAGAC] # 34 413 # # BccI: # [CCATC] # 139 308 # # BceAI: # [ACGGC] # 110 337 # # BinI*: # [GGATC] # 162 285 # # BisI: # [GCNGC] # 3 13 96 97 238 # # BlsI: # [GCNGC] # 3 13 96 97 238 # # BsaAI: # [YACGTR] # 200 247 # # BseBI: # [CCWGG] # 67 380 # # BseMII: # [CTCAG] # 162 285 # # BseRI: # [GAGGAG] # 193 254 # # BseSI: # [GKGCMC] # 7 440 # # BsgI: # [GTGCAG] # 216 231 # # BsiYI*: # [CCNNNNNNNGG] # 159 288 # # BsmAI: # [GTCTC] # 92 355 # # BspCNI: # [CTCAG] # 163 284 # # BspHI: # [TCATGA] # 5 442 # # BssKI: # [CCNGG] # 65 382 # # BstEII: # [GGTNACC] # 148 299 # # BstKTI: # [GATC] # 95 167 185 # # BtgZI: # [GCGATG] # 208 239 # # Cac8I: # [GCNNGC] # 4 79 364 # # Csp6I: # [GTAC] # 144 303 # # CviAII: # [CATG] # 4 70 373 # # CviJI: # [RGCY] # 18 65 81 136 147 # # CviRI*: # [TGCA] # 212 235 # # DdeI: # [CTNAG] # 176 271 # # DpnI: # [GATC] # 95 168 184 # # EcoP15I: # [CAGCAG] # 140 307 # # EcoRII: # [CCWGG] # 65 382 # # FatI: # [CATG] # 5 70 372 # # FnuDII*: # [CGCG] # 95 352 # # GlaI: # [GCGC] # 115 332 # # HgaI: # [GACGC] # 122 325 # # HhaI: # [GCGC] # 114 333 # # Hin4I: # [GAYNNNNNVTC] # 1 32 32 174 208 # # Hin6I: # [GCGC] # 116 331 # # HinfI: # [GANTC] # 26 102 319 # # HphI: # [GGTGA] # 142 305 # # Hpy178III*: # [TCNNGA] # 4 443 # # Hpy188I: # [TCNGA] # 18 177 252 # # MaeI: # [CTAG] # 217 230 # # MaeII: # [ACGT] # 38 201 208 # # MaeIII: # [GTNAC] # 28 84 148 187 # # MboI: # [GATC] # 95 170 182 # # MboII: # [GAAGA] # 2 12 15 176 242 # # MnlI: # [CCTC] # 18 20 171 238 # # MseI: # [TTAA] # 19 21 50 89 121 147 # # NlaIII: # [CATG] # 1 70 376 # # PshAI: # [GACNNNNGTC] # 84 363 # # RsaI: # [GTAC] # 143 304 # # ScrFI: # [CCNGG] # 67 380 # # SduI: # [GDGCHC] # 7 440 # # SecI*: # [CCNNGG] # 66 110 271 # # SetI: # [ASST] # 41 79 119 208 # # TaiI: # [ACGT] # 41 198 208 # # TaqI: # [TCGA] # 31 416 # # TatI: # [WGTACW] # 145 302 # # TauI: # [GCSGC] # 95 98 254 # # TfiI: # [GAWTC] # 26 102 319 # # TseI: # [GCWGC] # 13 101 333 # # Tsp45I: # [GTSAC] # 112 148 187 # # Tsp4CI*: # [ACNGT] # 53 394 # # TspDTI: # [ATGAA] # 31 416 # # TspEI: # [AATT] # 74 373 # # TspRI: # [CASTGNN] # 168 279 # # Tth111I: # [GACNNNGTC] # 53 394 # # VspI: # [ATTAAT] # 19 139 289 # # XcmI: # [CCANNNNNNNNNTGG] # 73 374 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 447 # Reported_sequences: 1 # Reported_hitcount: 116 #---------------------------------------