Restriction Fragments for WSS1/YHR134W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

WSS1/YHR134W on chromosome VIII from coordinates 371749 to 372558.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.21046.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 1 5 + TspDTI ATGAA 16 14 . . 3 7 + MboII GAAGA 15 14 . . 3 8 + BbvII* GAAGAC 10 14 . . 10 14 - Hin4II* CCTTC 4 3 . . 22 25 + CviJI RGCY 23 23 . . 25 29 + BccI CCATC 33 34 . . 39 49 + BsiYI* CCNNNNNNNGG 45 42 . . 39 49 + PflMI CCANNNNNTGG 45 42 . . 40 43 + FatI CATG 39 43 . . 40 43 + NlaIII CATG 43 39 . . 40 43 + CviAII CATG 40 42 . . 49 54 + Cac8I GCNNGC 51 51 . . 51 56 + SduI GDGCHC 55 51 . . 51 56 + HgiJII* GRGCYC 55 51 . . 52 55 + CviJI RGCY 53 53 . . 52 57 + NlaIV GGNNCC 54 54 . . 49 52 - AciI CCGC 49 51 . . 49 53 - FauI CCCGC 42 44 . . 60 70 + BplI GAGNNNNNCTC 51 46 83 78 62 66 + TfiI GAWTC 62 65 . . 62 66 + HinfI GANTC 62 65 . . 67 70 + MnlI CCTC 77 76 . . 60 70 - BplI GAGNNNNNCTC 83 78 51 46 78 82 + FauI CCCGC 86 88 . . 79 82 + AciI CCGC 79 81 . . 96 99 + CviJI RGCY 97 97 . . 104 107 + CviRI* TGCA 105 105 . . 105 115 + MwoI GCNNNNNNNGC 111 108 . . 113 116 + CviJI RGCY 114 114 . . 124 128 + MboII GAAGA 136 135 . . 130 133 + Hin6I GCGC 130 132 . . 130 133 + GlaI GCGC 131 131 . . 130 133 + HhaI GCGC 132 130 . . 127 131 - SfaNI GCATC 117 121 . . 135 140 + ApoI RAATTY 135 139 . . 136 139 + TspEI AATT 135 139 . . 139 142 + MseI TTAA 139 141 . . 175 179 + TspDTI ATGAA 190 188 . . 177 181 - Hin4II* CCTTC 171 170 . . 196 200 + Tsp45I GTSAC 195 200 . . 196 200 + MaeIII GTNAC 195 200 . . 200 211 + BstXI CCANNNNNNTGG 207 203 . . 221 226 + BsaAI YACGTR 223 223 . . 221 226 + PmaCI CACGTG 223 223 . . 222 225 + TaiI ACGT 225 221 . . 222 225 + SetI ASST 225 221 . . 222 225 + MaeII ACGT 222 224 . . 225 230 + BclI TGATCA 225 229 . . 226 229 + DpnI GATC 227 227 . . 226 229 + BstKTI GATC 228 226 . . 226 229 + MboI GATC 225 229 . . 233 237 + HinfI GANTC 233 236 . . 233 237 - PleI GAGTC 227 228 . . 233 237 - MlyI GAGTC 227 227 . . 237 241 + BssKI CCNGG 236 241 . . 237 241 + BseBI CCWGG 238 239 . . 237 242 + SecI* CCNNGG 237 241 . . 237 241 + ScrFI CCNGG 238 239 . . 237 241 + EcoRII CCWGG 236 241 . . 244 248 + TspDTI ATGAA 259 257 . . 248 251 + TaiI ACGT 251 247 . . 248 251 + SetI ASST 251 247 . . 248 251 + MaeII ACGT 248 250 . . 250 255 + Hpy166II GTNNAC 252 252 . . 250 255 + HindII GTYRAC 252 252 . . 255 260 + DsaI* CCRYGG 255 259 . . 255 260 + StyI CCWWGG 255 259 . . 255 260 + NcoI CCATGG 255 259 . . 255 260 + SecI* CCNNGG 255 259 . . 256 259 + FatI CATG 255 259 . . 256 259 + NlaIII CATG 259 255 . . 256 259 + CviAII CATG 256 258 . . 259 264 + XhoII RGATCY 259 263 . . 259 263 + BinI* GGATC 267 268 . . 260 263 + DpnI GATC 261 261 . . 260 263 + BstKTI GATC 262 260 . . 260 263 + MboI GATC 259 263 . . 263 267 + DdeI CTNAG 263 266 . . 273 277 + MaeIII GTNAC 272 277 . . 276 280 + Tsp4CI* ACNGT 278 277 . . 280 283 + MseI TTAA 280 282 . . 284 288 - SfaNI GCATC 274 278 . . 290 295 + DsaI* CCRYGG 290 294 . . 290 295 + SecI* CCNNGG 290 294 . . 292 296 + TspGWI ACGGA 307 305 . . 294 298 + FokI GGATG 307 311 . . 294 298 + BseGI GGATG 300 298 . . 305 308 + TspEI AATT 304 308 . . 314 319 + DsaI* CCRYGG 314 318 . . 314 319 + StyI CCWWGG 314 318 . . 314 319 + NcoI CCATGG 314 318 . . 314 319 + SecI* CCNNGG 314 318 . . 315 318 + FatI CATG 314 318 . . 315 318 + NlaIII CATG 318 314 . . 315 318 + CviAII CATG 315 317 . . 330 335 + SduI GDGCHC 334 330 . . 330 335 + BseSI GKGCMC 334 330 . . 331 336 + NlaIV GGNNCC 333 333 . . 331 336 + HgiCI* GGYRCC 331 335 . . 332 343 + AlfI GCANNNNNNTGC 321 319 355 353 336 339 + FatI CATG 335 339 . . 336 339 + NlaIII CATG 339 335 . . 336 339 + CviAII CATG 336 338 . . 339 343 + BlsI GCNGC 341 340 . . 339 343 + BisI GCNGC 340 341 . . 339 343 + TseI GCWGC 339 342 . . 341 344 + CviRI* TGCA 342 342 . . 339 343 - BbvI GCAGC 326 330 . . 332 343 - AlfI GCANNNNNNTGC 355 353 321 319 340 345 - BsgI GTGCAG 325 323 . . 347 350 + TspEI AATT 346 350 . . 349 352 + MseI TTAA 349 351 . . 358 361 + TspEI AATT 357 361 . . 367 371 + BmgT120I GGNCC 368 369 . . 367 371 + AvaII GGWCC 367 370 . . 367 371 + AsuI* GGNCC 367 370 . . 368 373 + Hpy166II GTNNAC 370 370 . . 365 370 - TaqII GACCGA 355 353 . . 395 398 + SetI ASST 398 394 . . 395 398 + CviJI RGCY 396 396 . . 395 398 + AluI AGCT 396 396 . . 397 400 + MaeI CTAG 397 399 . . 413 417 - Hin4II* CCTTC 407 406 . . 417 422 + BsrDI GCAATG 424 422 . . 438 441 + SetI ASST 441 437 . . 437 440 - MnlI CCTC 430 429 . . 444 447 + Csp6I GTAC 444 446 . . 444 447 + RsaI GTAC 445 445 . . 464 468 + Tsp4CI* ACNGT 466 465 . . 467 472 + Hpy166II GTNNAC 469 469 . . 467 472 + HindII GTYRAC 469 469 . . 471 474 + TaiI ACGT 474 470 . . 471 474 + SetI ASST 474 470 . . 471 474 + MaeII ACGT 471 473 . . 472 477 + Esp3I CGTCTC 478 482 . . 473 477 + BsmAI GTCTC 478 482 . . 482 486 + MboII GAAGA 494 493 . . 482 487 - Ksp632I* CTCTTC 477 480 . . 482 488 - SapI GCTCTTC 477 480 . . 495 498 + TaiI ACGT 498 494 . . 495 498 + SetI ASST 498 494 . . 495 498 + MaeII ACGT 495 497 . . 506 511 + EcoRV GATATC 508 508 . . 511 521 + EcoNI CCTNNNNNAGG 515 516 . . 511 521 + BsiYI* CCNNNNNNNGG 517 514 . . 526 531 + TatI WGTACW 526 530 . . 526 531 + ScaI AGTACT 528 528 . . 527 530 + Csp6I GTAC 527 529 . . 527 530 + RsaI GTAC 528 528 . . 561 564 + SetI ASST 564 560 . . 581 584 + CviRI* TGCA 582 582 . . 582 586 + SfaNI GCATC 591 595 . . 585 590 + Hpy178III* TCNNGA 586 588 . . 576 586 - Hin4I GAYNNNNNVTC 599 594 567 562 583 587 - FokI GGATG 569 573 . . 583 587 - BseGI GGATG 582 580 . . 589 592 - MnlI CCTC 582 581 . . 589 599 - Hin4I GAYNNNNNVTC 612 607 580 575 602 607 + SfeI* CTRYAG 602 606 . . 612 615 + SetI ASST 615 611 . . 611 614 - MnlI CCTC 604 603 . . 616 619 + TspEI AATT 615 619 . . 621 626 + BmeT110I CYCGRG 622 624 . . 621 626 + AvaI CYCGRG 621 625 . . 634 638 + TauI GCSGC 637 634 . . 634 644 + MwoI GCNNNNNNNGC 640 637 . . 634 638 + BlsI GCNGC 636 635 . . 634 638 + BisI GCNGC 635 636 . . 636 639 + CviJI RGCY 637 637 . . 637 647 + MwoI GCNNNNNNNGC 643 640 . . 634 637 - AciI CCGC 634 636 . . 642 645 + CviRI* TGCA 643 643 . . 643 647 + BlsI GCNGC 645 644 . . 643 647 + BisI GCNGC 644 645 . . 643 647 + BbvI GCAGC 655 659 . . 643 647 + TseI GCWGC 643 646 . . 645 648 + CviJI RGCY 646 646 . . 667 670 + DpnI GATC 668 668 . . 667 670 + BstKTI GATC 669 667 . . 667 670 + MboI GATC 666 670 . . 677 680 - AciI CCGC 677 679 . . 682 686 - BsmAI GTCTC 676 680 . . 687 691 + MboII GAAGA 699 698 . . 682 687 - Esp3I CGTCTC 676 680 . . 700 703 + DpnI GATC 701 701 . . 700 703 + BstKTI GATC 702 700 . . 700 703 + MboI GATC 699 703 . . 718 723 + SspI AATATT 720 720 . . 725 729 + BlsI GCNGC 727 726 . . 725 729 + BisI GCNGC 726 727 . . 725 729 + BbvI GCAGC 737 741 . . 725 729 + TseI GCWGC 725 728 . . 727 730 + SetI ASST 730 726 . . 727 730 + CviJI RGCY 728 728 . . 727 730 + AluI AGCT 728 728 . . 732 736 + DdeI CTNAG 732 735 . . 764 767 + SetI ASST 767 763 . . 763 766 - MnlI CCTC 756 755 . . 766 769 + Csp6I GTAC 766 768 . . 766 769 + RsaI GTAC 767 767 . . 771 776 + SmaI CCCGGG 773 773 . . 771 776 + Cfr9I CCCGGG 771 775 . . 771 775 + BssKI CCNGG 770 775 . . 771 776 + SecI* CCNNGG 771 775 . . 771 776 + BmeT110I CYCGRG 772 774 . . 771 775 + ScrFI CCNGG 772 773 . . 771 775 + CauII* CCSGG 772 773 . . 771 776 + AvaI CYCGRG 771 775 . . 772 776 + BssKI CCNGG 771 776 . . 772 775 + HpaII CCGG 772 774 . . 772 776 + ScrFI CCNGG 773 774 . . 772 776 + CauII* CCSGG 773 774 . . 788 791 + TaqI TCGA 788 790 . . 799 802 + DpnI GATC 800 800 . . 799 802 + BstKTI GATC 801 799 . . 799 802 + MboI GATC 798 802 . . 807 810 + MseI TTAA 807 809 . . #--------------------------------------- # # # AciI: # [CCGC] # 30 43 49 133 555 # # AlfI: # [GCANNNNNNTGC] # 0 0 34 321 455 # # AluI: # [AGCT] # 82 332 396 # # ApoI: # [RAATTY] # 135 675 # # AsuI*: # [GGNCC] # 367 443 # # AvaI: # [CYCGRG] # 39 150 621 # # AvaII: # [GGWCC] # 367 443 # # BbvI: # [GCAGC] # 73 82 326 329 # # BbvII*: # [GAAGAC] # 10 800 # # BccI: # [CCATC] # 33 777 # # BclI: # [TGATCA] # 225 585 # # BinI*: # [GGATC] # 267 543 # # BisI: # [GCNGC] # 9 82 84 295 340 # # BlsI: # [GCNGC] # 9 82 83 295 341 # # BmeT110I: # [CYCGRG] # 38 150 622 # # BmgT120I: # [GGNCC] # 368 442 # # BplI: # [GAGNNNNNCTC] # 0 0 32 51 727 # # BsaAI: # [YACGTR] # 223 587 # # BseBI: # [CCWGG] # 238 572 # # BseGI: # [GGATG] # 228 282 300 # # BseSI: # [GKGCMC] # 334 476 # # BsgI: # [GTGCAG] # 325 485 # # BsiYI*: # [CCNNNNNNNGG] # 45 293 472 # # BsmAI: # [GTCTC] # 134 198 478 # # BsrDI: # [GCAATG] # 386 424 # # BssKI: # [CCNGG] # 1 39 236 534 # # BstKTI: # [GATC] # 9 33 34 99 228 407 # # BstXI: # [CCANNNNNNTGG] # 207 603 # # Cac8I: # [GCNNGC] # 51 759 # # CauII*: # [CCSGG] # 1 37 772 # # Cfr9I: # [CCCGGG] # 39 771 # # Csp6I: # [GTAC] # 44 83 239 444 # # CviAII: # [CATG] # 21 40 59 216 474 # # CviJI: # [RGCY] # 9 17 23 30 44 82 # 82 241 282 # # CviRI*: # [TGCA] # 61 105 167 237 240 # # DdeI: # [CTNAG] # 78 263 469 # # DpnI: # [GATC] # 10 33 34 99 227 407 # # DsaI*: # [CCRYGG] # 24 35 255 496 # # EcoNI: # [CCTNNNNNAGG] # 295 515 # # EcoRII: # [CCWGG] # 236 574 # # EcoRV: # [GATATC] # 302 508 # # Esp3I: # [CGTCTC] # 134 198 478 # # FatI: # [CATG] # 21 39 59 216 475 # # FauI: # [CCCGC] # 42 44 724 # # FokI: # [GGATG] # 241 262 307 # # GlaI: # [GCGC] # 131 679 # # HgiCI*: # [GGYRCC] # 331 479 # # HgiJII*: # [GRGCYC] # 55 755 # # HhaI: # [GCGC] # 132 678 # # Hin4I: # [GAYNNNNNVTC] # 13 13 19 198 567 # # Hin4II*: # [CCTTC] # 4 167 236 403 # # Hin6I: # [GCGC] # 130 680 # # HindII: # [GTYRAC] # 217 252 341 # # HinfI: # [GANTC] # 62 171 577 # # HpaII: # [CCGG] # 38 772 # # Hpy166II: # [GTNNAC] # 99 118 252 341 # # Hpy178III*: # [TCNNGA] # 224 586 # # Ksp632I*: # [CTCTTC] # 333 477 # # MaeI: # [CTAG] # 397 413 # # MaeII: # [ACGT] # 24 26 222 223 315 # # MaeIII: # [GTNAC] # 77 195 538 # # MboI: # [GATC] # 12 33 34 99 225 407 # # MboII: # [GAAGA] # 15 111 121 205 358 # # MlyI: # [GAGTC] # 227 583 # # MnlI: # [CCTC] # 22 54 77 152 152 353 # # MseI: # [TTAA] # 3 69 139 141 458 # # MwoI: # [GCNNNNNNNGC] # 3 111 167 529 # # NcoI: # [CCATGG] # 59 255 496 # # NlaIII: # [CATG] # 21 43 59 216 471 # # NlaIV: # [GGNNCC] # 54 279 477 # # PflMI: # [CCANNNNNTGG] # 45 765 # # PleI: # [GAGTC] # 227 583 # # PmaCI: # [CACGTG] # 223 587 # # RsaI: # [GTAC] # 43 83 239 445 # # SapI: # [GCTCTTC] # 333 477 # # ScaI: # [AGTACT] # 282 528 # # ScrFI: # [CCNGG] # 1 37 238 534 # # SduI: # [GDGCHC] # 55 279 476 # # SecI*: # [CCNNGG] # 18 24 35 39 237 457 # # SetI: # [ASST] # 24 26 33 37 43 43 # 51 66 115 147 225 # # SfaNI: # [GCATC] # 117 157 219 317 # # SfeI*: # [CTRYAG] # 208 602 # # SmaI: # [CCCGGG] # 37 773 # # SspI: # [AATATT] # 90 720 # # StyI: # [CCWWGG] # 59 255 496 # # TaiI: # [ACGT] # 24 26 223 225 312 # # TaqI: # [TCGA] # 22 788 # # TaqII: # [GACCGA] # 355 455 # # TatI: # [WGTACW] # 284 526 # # TauI: # [GCSGC] # 173 637 # # TfiI: # [GAWTC] # 62 748 # # TseI: # [GCWGC] # 82 85 304 339 # # Tsp45I: # [GTSAC] # 195 615 # # Tsp4CI*: # [ACNGT] # 188 278 344 # # TspDTI: # [ATGAA] # 16 69 174 551 # # TspEI: # [AATT] # 11 42 135 169 195 258 # # TspGWI: # [ACGGA] # 307 503 # # XhoII: # [RGATCY] # 259 551 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 810 # Reported_sequences: 1 # Reported_hitcount: 217 #---------------------------------------