Restriction Fragments for FUR1/YHR128W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

FUR1/YHR128W on chromosome VIII from coordinates 362115 to 362765.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.29112.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 7 11 + Hpy188I TCNGA 9 8 . . 9 14 + NlaIV GGNNCC 11 11 . . 17 20 + MseI TTAA 17 19 . . 23 26 + TaiI ACGT 26 22 . . 23 26 + SetI ASST 26 22 . . 23 26 + MaeII ACGT 23 25 . . 25 30 + Hpy166II GTNNAC 27 27 . . 25 30 + AccI GTMKAC 26 28 . . 36 39 + SetI ASST 39 35 . . 37 40 + MnlI CCTC 47 46 . . 48 58 + BsiYI* CCNNNNNNNGG 54 51 . . 48 58 + PflMI CCANNNNNTGG 54 51 . . 49 54 + MfeI CAATTG 49 53 . . 50 53 + TspEI AATT 49 53 . . 54 59 - GsaI CCCAGC 58 54 . . 54 59 - BseYI CCCAGC 54 58 . . 62 67 + TatI WGTACW 62 66 . . 62 67 + Bsp1407I TGTACA 62 66 . . 63 68 + Hpy166II GTNNAC 65 65 . . 63 66 + Csp6I GTAC 63 65 . . 63 66 + RsaI GTAC 64 64 . . 68 72 + BccI CCATC 76 77 . . 74 78 + Hpy188I TCNGA 76 75 . . 92 95 + MaeI CTAG 92 94 . . 96 99 + SetI ASST 99 95 . . 103 107 - TspDTI ATGAA 93 91 . . 115 119 + Hpy188I TCNGA 117 116 . . 122 126 + TfiI GAWTC 122 125 . . 122 126 + HinfI GANTC 122 125 . . 128 132 + Hpy188I TCNGA 130 129 . . 142 146 + MboII GAAGA 154 153 . . 147 150 + SetI ASST 150 146 . . 145 149 - Hin4II* CCTTC 139 138 . . 156 160 + BccI CCATC 164 165 . . 162 165 + SetI ASST 165 161 . . 167 170 + CviRI* TGCA 168 168 . . 177 180 + TspEI AATT 176 180 . . 205 210 + AsuII TTCGAA 206 208 . . 206 209 + TaqI TCGA 206 208 . . 194 207 - AjuI GAANNNNNNNTTGG 219 214 187 182 210 213 + SetI ASST 213 209 . . 208 212 - Hin4II* CCTTC 202 201 . . 214 218 + BsmAI GTCTC 219 223 . . 222 225 + FatI CATG 221 225 . . 222 225 + NlaIII CATG 225 221 . . 222 225 + CviAII CATG 222 224 . . 220 224 - TspDTI ATGAA 210 208 . . 251 255 + Hpy188I TCNGA 253 252 . . 255 258 + SetI ASST 258 254 . . 255 258 + CviJI RGCY 256 256 . . 255 258 + AluI AGCT 256 256 . . 259 263 + HphI GGTGA 271 270 . . 262 266 + TfiI GAWTC 262 265 . . 262 266 + HinfI GANTC 262 265 . . 264 269 + ClaI ATCGAT 265 267 . . 265 268 + TaqI TCGA 265 267 . . 267 271 - BccI CCATC 261 262 . . 280 283 + MseI TTAA 280 282 . . 287 291 + Tsp4CI* ACNGT 289 288 . . 284 288 - BsmAI GTCTC 278 282 . . 295 298 + SetI ASST 298 294 . . 314 319 + ApoI RAATTY 314 318 . . 315 318 + TspEI AATT 314 318 . . 319 322 + MseI TTAA 319 321 . . 321 324 + TspEI AATT 320 324 . . 329 333 + BslFI GGGAC 343 347 . . 333 344 + BcgI CGANNNNNNTGC 322 320 356 354 334 339 + BseRI GAGGAG 349 347 . . 334 337 - MnlI CCTC 327 326 . . 337 341 - BsmAI GTCTC 331 335 . . 368 371 + TspEI AATT 367 371 . . 376 379 - MnlI CCTC 369 368 . . 385 389 + Hpy188I TCNGA 387 386 . . 376 386 - Hin4I GAYNNNNNVTC 399 394 367 362 391 394 + SetI ASST 394 390 . . 398 402 - MboII GAAGA 390 389 . . 397 402 - BbvII* GAAGAC 390 394 . . 411 421 + BsiYI* CCNNNNNNNGG 417 414 . . 417 422 + Cac8I GCNNGC 419 419 . . 419 424 + BalI TGGCCA 421 421 . . 419 424 + CfrI YGGCCR 419 423 . . 420 423 + CviJI RGCY 421 421 . . 420 423 + HaeIII GGCC 421 421 . . 423 430 + SgrAI CRCCGGYG 424 428 . . 424 429 + Cfr10I RCCGGY 424 428 . . 424 429 + BetI* WCCGGW 424 428 . . 424 429 + AgeI ACCGGT 424 428 . . 425 428 + HpaII CCGG 425 427 . . 436 446 + MwoI GCNNNNNNNGC 442 439 . . 441 444 + FatI CATG 440 444 . . 441 444 + NlaIII CATG 444 440 . . 441 444 + CviAII CATG 441 443 . . 444 447 + CviJI RGCY 445 445 . . 446 451 + SfeI* CTRYAG 446 450 . . 443 448 - TsoI TARCCA 433 431 . . 455 460 + Hpy178III* TCNNGA 456 458 . . 461 464 + MseI TTAA 461 463 . . 468 471 + SetI ASST 471 467 . . 467 470 - MnlI CCTC 460 459 . . 473 476 + MseI TTAA 473 475 . . 476 479 + CviJI RGCY 477 477 . . 485 490 + ApoI RAATTY 485 489 . . 486 489 + TspEI AATT 485 489 . . 496 499 + MseI TTAA 496 498 . . 500 503 + SetI ASST 503 499 . . 507 512 + SfeI* CTRYAG 507 511 . . 517 521 - Hin4II* CCTTC 511 510 . . 535 538 + FatI CATG 534 538 . . 535 538 + NlaIII CATG 538 534 . . 535 538 + CviAII CATG 535 537 . . 538 542 + TauI GCSGC 541 538 . . 538 542 + BlsI GCNGC 540 539 . . 538 542 + BisI GCNGC 539 540 . . 539 542 + AciI CCGC 539 541 . . 542 546 + Hin4II* CCTTC 552 551 . . 551 554 + SetI ASST 554 550 . . 550 553 - MnlI CCTC 543 542 . . 554 558 + Hpy188I TCNGA 556 555 . . 558 561 + TspEI AATT 557 561 . . 562 566 + MaeIII GTNAC 561 566 . . 565 569 + BsrI ACTGG 570 568 . . 568 573 + NlaIV GGNNCC 570 570 . . 568 573 + HgiCI* GGYRCC 568 572 . . 569 574 + SduI GDGCHC 573 569 . . 569 574 + BseSI GKGCMC 573 569 . . 573 576 + MnlI CCTC 583 582 . . 575 578 + TaqI TCGA 575 577 . . 577 586 + PshAI GACNNNNGTC 581 581 . . 582 585 + SetI ASST 585 581 . . 581 584 - MnlI CCTC 574 573 . . 585 590 + XbaI TCTAGA 585 589 . . 585 590 + Hpy178III* TCNNGA 586 588 . . 586 589 + MaeI CTAG 586 588 . . 590 594 + TspDTI ATGAA 605 603 . . 604 607 + MaeI CTAG 604 606 . . 610 614 + BssKI CCNGG 609 614 . . 610 614 + BseBI CCWGG 611 612 . . 610 615 + SecI* CCNNGG 610 614 . . 610 614 + ScrFI CCNGG 611 612 . . 610 614 + EcoRII CCWGG 609 614 . . 610 620 + BsiYI* CCNNNNNNNGG 616 613 . . 619 623 + HphI GGTGA 631 630 . . 620 624 + Tsp45I GTSAC 619 624 . . 620 624 + MaeIII GTNAC 619 624 . . 617 622 - TaqII CACCCA 607 605 . . 628 632 + HphI GGTGA 640 639 . . 629 633 + Tsp45I GTSAC 628 633 . . 629 633 + MaeIII GTNAC 628 633 . . 641 645 + Tsp4CI* ACNGT 643 642 . . 648 651 + MseI TTAA 648 650 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 26 625 # # AciI: # [CCGC] # 112 539 # # AgeI: # [ACCGGT] # 227 424 # # AjuI: # [GAANNNNNNNTTGG] # 32 187 432 # # AluI: # [AGCT] # 256 395 # # ApoI: # [RAATTY] # 166 171 314 # # AsuII: # [TTCGAA] # 206 445 # # BalI: # [TGGCCA] # 230 421 # # BbvII*: # [GAAGAC] # 261 390 # # BccI: # [CCATC] # 76 88 97 390 # # BcgI: # [CGANNNNNNTGC] # 34 295 322 # # BetI*: # [WCCGGW] # 227 424 # # BisI: # [GCNGC] # 112 539 # # BlsI: # [GCNGC] # 111 540 # # BseBI: # [CCWGG] # 40 611 # # BseRI: # [GAGGAG] # 302 349 # # BseSI: # [GKGCMC] # 78 573 # # BseYI: # [CCCAGC] # 54 597 # # BsiYI*: # [CCNNNNNNNGG] # 35 54 199 363 # # BslFI: # [GGGAC] # 308 343 # # BsmAI: # [GTCTC] # 53 59 219 320 # # Bsp1407I: # [TGTACA] # 62 589 # # BsrI: # [ACTGG] # 81 570 # # BssKI: # [CCNGG] # 42 609 # # Cac8I: # [GCNNGC] # 232 419 # # Cfr10I: # [RCCGGY] # 227 424 # # CfrI: # [YGGCCR] # 232 419 # # ClaI: # [ATCGAT] # 265 386 # # Csp6I: # [GTAC] # 63 588 # # CviAII: # [CATG] # 94 116 219 222 # # CviJI: # [RGCY] # 24 32 165 174 256 # # CviRI*: # [TGCA] # 168 483 # # EcoRII: # [CCWGG] # 42 609 # # FatI: # [CATG] # 94 117 219 221 # # GsaI: # [CCCAGC] # 58 593 # # HaeIII: # [GGCC] # 230 421 # # HgiCI*: # [GGYRCC] # 83 568 # # Hin4I: # [GAYNNNNNVTC] # 32 252 367 # # Hin4II*: # [CCTTC] # 41 63 99 139 309 # # HinfI: # [GANTC] # 122 140 389 # # HpaII: # [CCGG] # 226 425 # # HphI: # [GGTGA] # 9 11 271 360 # # Hpy166II: # [GTNNAC] # 27 38 586 # # Hpy178III*: # [TCNNGA] # 65 130 456 # # Hpy188I: # [TCNGA] # 9 13 41 67 95 123 # 134 169 # # MaeI: # [CTAG] # 18 47 92 494 # # MaeII: # [ACGT] # 23 628 # # MaeIII: # [GTNAC] # 9 23 58 561 # # MboII: # [GAAGA] # 154 236 261 # # MfeI: # [CAATTG] # 49 602 # # MnlI: # [CCTC] # 9 31 42 47 68 83 # 91 280 # # MseI: # [TTAA] # 3 12 17 23 39 142 # 152 263 # # MwoI: # [GCNNNNNNNGC] # 209 442 # # NlaIII: # [CATG] # 94 113 219 225 # # NlaIV: # [GGNNCC] # 11 81 559 # # PflMI: # [CCANNNNNTGG] # 54 597 # # PshAI: # [GACNNNNGTC] # 70 581 # # RsaI: # [GTAC] # 64 587 # # ScrFI: # [CCNGG] # 40 611 # # SduI: # [GDGCHC] # 78 573 # # SecI*: # [CCNNGG] # 41 610 # # SetI: # [ASST] # 13 15 26 31 32 40 # 45 48 51 51 60 66 # 77 96 # # SfeI*: # [CTRYAG] # 61 144 446 # # SgrAI: # [CRCCGGYG] # 227 424 # # TaiI: # [ACGT] # 26 625 # # TaqI: # [TCGA] # 59 76 206 310 # # TaqII: # [CACCCA] # 44 607 # # TatI: # [WGTACW] # 62 589 # # TauI: # [GCSGC] # 110 541 # # TfiI: # [GAWTC] # 122 140 389 # # TsoI: # [TARCCA] # 218 433 # # Tsp45I: # [GTSAC] # 9 23 619 # # Tsp4CI*: # [ACNGT] # 8 289 354 # # TspDTI: # [ATGAA] # 46 93 117 395 # # TspEI: # [AATT] # 6 47 49 72 94 118 # 127 138 # # XbaI: # [TCTAGA] # 66 585 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 651 # Reported_sequences: 1 # Reported_hitcount: 150 #---------------------------------------