Restriction Fragments for IRE1/YHR079C



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The currently selected gene/sequence is :

IRE1/YHR079C on chromosome VIII from coordinates 261591 to 258244.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.23310.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 5 10 + Hpy166II GTNNAC 7 7 . . 5 10 + AccI GTMKAC 6 8 . . 11 16 + AsuII TTCGAA 12 14 . . 12 15 + TaqI TCGA 12 14 . . 14 18 + MboII GAAGA 26 25 . . 20 25 + BspLU11I* ACATGT 20 24 . . 20 25 + NspI RCATGY 24 20 . . 20 25 + AflIII ACRYGT 20 24 . . 21 24 + FatI CATG 20 24 . . 21 24 + NlaIII CATG 24 20 . . 21 24 + CviAII CATG 21 23 . . 35 41 + TspRI CASTGNN 41 32 . . 35 40 - BtsI GCAGTG 34 32 . . 54 58 - TspDTI ATGAA 44 42 . . 59 63 + BccI CCATC 67 68 . . 56 60 - FokI GGATG 42 46 . . 56 60 - BseGI GGATG 55 53 . . 68 71 + FatI CATG 67 71 . . 68 71 + NlaIII CATG 71 67 . . 68 71 + CviAII CATG 68 70 . . 66 70 - TspDTI ATGAA 56 54 . . 86 91 + Esp3I CGTCTC 92 96 . . 87 91 + BsmAI GTCTC 92 96 . . 94 97 + SetI ASST 97 93 . . 95 98 + MnlI CCTC 105 104 . . 95 105 + BsiYI* CCNNNNNNNGG 101 98 . . 96 101 + SmlI CTYRAG 96 100 . . 96 101 - Bce83I* CTTGAG 81 79 . . 102 106 + TauI GCSGC 105 102 . . 102 106 + BlsI GCNGC 104 103 . . 102 106 + BisI GCNGC 103 104 . . 102 105 - AciI CCGC 102 104 . . 115 119 + MboII GAAGA 127 126 . . 119 123 + TspDTI ATGAA 134 132 . . 128 131 + MnlI CCTC 138 137 . . 140 143 + SetI ASST 143 139 . . 140 143 + CviJI RGCY 141 141 . . 140 143 + AluI AGCT 141 141 . . 145 148 + TspEI AATT 144 148 . . 201 207 + TspRI CASTGNN 207 198 . . 203 208 + Eco57MI CTGRAG 224 222 . . 203 208 + Eco57I CTGAAG 224 222 . . 207 210 + SetI ASST 210 206 . . 205 209 - Hin4II* CCTTC 199 198 . . 209 214 + Hpy166II GTNNAC 211 211 . . 220 224 + TspDTI ATGAA 235 233 . . 226 230 + DdeI CTNAG 226 229 . . 226 230 + BspCNI CTCAG 239 237 . . 226 230 + BseMII CTCAG 240 238 . . 229 232 + SetI ASST 232 228 . . 229 232 + CviJI RGCY 230 230 . . 229 232 + AluI AGCT 230 230 . . 237 242 + MmeI TCCRAC 262 260 . . 260 265 - EcoP15I CAGCAG 232 234 . . 270 273 + TaqI TCGA 270 272 . . 271 275 + Hpy99I CGWCG 275 270 . . 273 276 + TaiI ACGT 276 272 . . 273 276 + SetI ASST 276 272 . . 273 276 + MaeII ACGT 273 275 . . 272 277 - BtrI CACGTC 274 274 . . 291 294 + TaiI ACGT 294 290 . . 291 294 + SetI ASST 294 290 . . 291 294 + MaeII ACGT 291 293 . . 297 300 + CviJI RGCY 298 298 . . 298 302 + BlsI GCNGC 300 299 . . 298 302 + BisI GCNGC 299 300 . . 298 302 + TseI GCWGC 298 301 . . 298 302 - BbvI GCAGC 285 289 . . 304 307 + TspEI AATT 303 307 . . 314 319 + Hpy166II GTNNAC 316 316 . . 316 319 + Csp6I GTAC 316 318 . . 316 319 + RsaI GTAC 317 317 . . 330 334 + TfiI GAWTC 330 333 . . 330 334 + HinfI GANTC 330 333 . . 349 353 + DdeI CTNAG 349 352 . . 349 353 - BspCNI CTCAG 341 339 . . 349 353 - BseMII CTCAG 340 338 . . 358 362 + Hpy188I TCNGA 360 359 . . 373 377 + BlsI GCNGC 375 374 . . 373 377 + BisI GCNGC 374 375 . . 373 377 + BbvI GCAGC 385 389 . . 373 377 + TseI GCWGC 373 376 . . 375 378 + CviJI RGCY 376 376 . . 378 382 + Hpy99I CGWCG 382 377 . . 380 383 + TaiI ACGT 383 379 . . 380 383 + SetI ASST 383 379 . . 380 383 + MaeII ACGT 380 382 . . 385 388 - MnlI CCTC 378 377 . . 389 394 + Hpy166II GTNNAC 391 391 . . 397 400 + FatI CATG 396 400 . . 397 400 + NlaIII CATG 400 396 . . 397 400 + CviAII CATG 397 399 . . 395 399 - TspDTI ATGAA 385 383 . . 401 406 + SfeI* CTRYAG 401 405 . . 410 414 + MboII GAAGA 422 421 . . 426 431 + NdeI CATATG 427 429 . . 437 440 + TaqI TCGA 437 439 . . 445 454 + XmnI GAANNNNTTC 449 449 . . 447 452 + ApoI RAATTY 447 451 . . 448 451 + TspEI AATT 447 451 . . 456 459 + SetI ASST 459 455 . . 457 460 + MnlI CCTC 467 466 . . 459 463 + Hpy188I TCNGA 461 460 . . 477 480 + SetI ASST 480 476 . . 478 482 + Hin4II* CCTTC 488 487 . . 481 484 + TaqI TCGA 481 483 . . 484 487 + SetI ASST 487 483 . . 483 486 - MnlI CCTC 476 475 . . 494 499 + NdeI CATATG 495 497 . . 497 501 + TspDTI ATGAA 512 510 . . 501 506 + AclI AACGTT 502 504 . . 502 505 + TaiI ACGT 505 501 . . 502 505 + SetI ASST 505 501 . . 502 505 + MaeII ACGT 502 504 . . 516 519 + SetI ASST 519 515 . . 523 527 + HphI GGTGA 535 534 . . 526 530 - BccI CCATC 520 521 . . 531 543 + PsrI GAACNNNNNNTAC 523 518 555 550 545 548 + MseI TTAA 545 547 . . 551 561 + BsiYI* CCNNNNNNNGG 557 554 . . 552 556 + BccI CCATC 560 561 . . 561 565 + MaeIII GTNAC 560 565 . . 606 610 + FauI CCCGC 614 616 . . 607 612 + NspBII* CMGCKG 609 609 . . 607 610 + AciI CCGC 607 609 . . 609 613 + BlsI GCNGC 611 610 . . 609 613 + BisI GCNGC 610 611 . . 609 613 + TseI GCWGC 609 612 . . 611 614 + CviRI* TGCA 612 612 . . 609 613 - BbvI GCAGC 596 600 . . 610 615 - BsgI GTGCAG 595 593 . . 623 634 + CspCI CAANNNNNGTGG 611 609 646 644 625 630 + SspI AATATT 627 627 . . 635 638 + MseI TTAA 635 637 . . 632 637 - TsoI TARCCA 622 620 . . 640 644 + HinfI GANTC 640 643 . . 643 648 + Hpy178III* TCNNGA 644 646 . . 640 644 - PleI GAGTC 634 635 . . 640 644 - MlyI GAGTC 634 634 . . 651 654 + TspEI AATT 650 654 . . 658 662 + MboII GAAGA 670 669 . . 662 666 + TspDTI ATGAA 677 675 . . 668 671 + SetI ASST 671 667 . . 670 675 + Hpy166II GTNNAC 672 672 . . 670 675 + AccI GTMKAC 671 673 . . 675 681 + TspRI CASTGNN 681 672 . . 676 680 + BsrI ACTGG 681 679 . . 679 683 + BinI* GGATC 687 688 . . 680 683 + DpnI GATC 681 681 . . 680 683 + BstKTI GATC 682 680 . . 680 683 + MboI GATC 679 683 . . 681 686 + ClaI ATCGAT 682 684 . . 682 685 + TaqI TCGA 682 684 . . 699 704 + Hpy166II GTNNAC 701 701 . . 699 704 + BssNAI GTATAC 701 701 . . 699 704 + AccI GTMKAC 700 702 . . 710 715 + BspLU11I* ACATGT 710 714 . . 710 715 + NspI RCATGY 714 710 . . 710 715 + AflIII ACRYGT 710 714 . . 711 714 + FatI CATG 710 714 . . 711 714 + NlaIII CATG 714 710 . . 711 714 + CviAII CATG 711 713 . . 721 725 + HphI GGTGA 733 732 . . 726 729 + TspEI AATT 725 729 . . 740 744 + Hpy188I TCNGA 742 741 . . 742 746 + BmgT120I GGNCC 743 744 . . 742 746 + AvaII GGWCC 742 745 . . 742 746 + AsuI* GGNCC 742 745 . . 744 747 + SetI ASST 747 743 . . 744 750 + SexAI ACCWGGT 744 749 . . 745 749 + BssKI CCNGG 744 749 . . 745 749 + BseBI CCWGG 746 747 . . 745 749 + ScrFI CCNGG 746 747 . . 745 749 + EcoRII CCWGG 744 749 . . 767 772 + Hpy178III* TCNNGA 768 770 . . 770 775 + NlaIV GGNNCC 772 772 . . 772 775 + CviJI RGCY 773 773 . . 774 784 + BsiYI* CCNNNNNNNGG 780 777 . . 774 784 + PflMI CCANNNNNTGG 780 777 . . 770 780 - BsaXI ACNNNNNCTCC 792 789 762 759 770 781 - TstI CACNNNNNNTCC 794 789 762 757 792 795 + SetI ASST 795 791 . . 793 799 + SauI* CCTNAGG 794 797 . . 794 798 + DdeI CTNAG 794 797 . . 790 794 - HphI GGTGA 782 781 . . 796 801 + BseRI GAGGAG 811 809 . . 794 798 - BspCNI CTCAG 786 784 . . 794 798 - BseMII CTCAG 785 783 . . 796 799 - MnlI CCTC 789 788 . . 801 805 + MboII GAAGA 813 812 . . 813 818 + Hpy178III* TCNNGA 814 816 . . 811 816 - Eco57MI CTGRAG 796 794 . . 811 816 - Eco57I CTGAAG 796 794 . . 857 860 + SetI ASST 860 856 . . 857 860 + CviJI RGCY 858 858 . . 857 860 + AluI AGCT 858 858 . . 858 863 - GsaI CCCAGC 862 858 . . 863 868 + EcoRI GAATTC 863 867 . . 858 863 - BseYI CCCAGC 858 862 . . 863 868 + ApoI RAATTY 863 867 . . 864 867 + TspEI AATT 863 867 . . 868 877 + MslI CAYNNNNRTG 872 872 . . 877 881 - BccI CCATC 871 872 . . 883 888 + Cac8I GCNNGC 885 885 . . 886 889 + SetI ASST 889 885 . . 886 889 + CviJI RGCY 887 887 . . 886 889 + AluI AGCT 887 887 . . 895 899 + Tsp45I GTSAC 894 899 . . 895 899 + MaeIII GTNAC 894 899 . . 908 911 + FatI CATG 907 911 . . 908 911 + NlaIII CATG 911 907 . . 908 911 + CviAII CATG 908 910 . . 912 916 + BlsI GCNGC 914 913 . . 912 916 + BisI GCNGC 913 914 . . 912 916 + BbvI GCAGC 924 928 . . 912 916 + TseI GCWGC 912 915 . . 937 940 + MaeI CTAG 937 939 . . 939 944 + HaeII RGCGCY 943 939 . . 939 944 + Eco47III AGCGCT 941 941 . . 940 943 + Hin6I GCGC 940 942 . . 940 943 + GlaI GCGC 941 941 . . 940 943 + HhaI GCGC 942 940 . . 945 949 + Hpy188I TCNGA 947 946 . . 943 948 - Eco57MI CTGRAG 928 926 . . 943 948 - Eco57I CTGAAG 928 926 . . 965 969 + BceAI ACGGC 981 983 . . 968 973 + NspI RCATGY 972 968 . . 969 972 + FatI CATG 968 972 . . 969 972 + NlaIII CATG 972 968 . . 969 972 + CviAII CATG 969 971 . . 973 976 + CviRI* TGCA 974 974 . . 963 974 - TstI CACNNNNNNTCC 987 982 955 950 978 983 + HaeII RGCGCY 982 978 . . 979 982 + Hin6I GCGC 979 981 . . 979 982 + GlaI GCGC 980 980 . . 979 982 + HhaI GCGC 981 979 . . 986 990 - TspGWI ACGGA 976 974 . . 1002 1007 + Cac8I GCNNGC 1004 1004 . . 1002 1007 + BmtI GCTAGC 1006 1002 . . 1002 1007 + NheI GCTAGC 1002 1006 . . 998 1003 - BsrDI GCAATG 997 995 . . 1003 1006 + MaeI CTAG 1003 1005 . . 1006 1011 + Cac8I GCNNGC 1008 1008 . . 1026 1029 + TspEI AATT 1025 1029 . . 1031 1034 + MaeI CTAG 1031 1033 . . 1034 1038 - BccI CCATC 1028 1029 . . 1044 1049 + MmeI TCCRAC 1069 1067 . . 1044 1048 + Hpy188I TCNGA 1046 1045 . . 1053 1058 + SecI* CCNNGG 1053 1057 . . 1054 1058 + BssKI CCNGG 1053 1058 . . 1054 1058 + ScrFI CCNGG 1055 1056 . . 1054 1058 + CauII* CCSGG 1055 1056 . . 1055 1058 + HpaII CCGG 1055 1057 . . 1059 1062 + TspEI AATT 1058 1062 . . 1070 1073 + CviJI RGCY 1071 1071 . . 1076 1079 + TaqI TCGA 1076 1078 . . 1085 1088 + MseI TTAA 1085 1087 . . 1090 1093 + DpnI GATC 1091 1091 . . 1090 1093 + BstKTI GATC 1092 1090 . . 1090 1093 + MboI GATC 1089 1093 . . 1095 1098 + AciI CCGC 1095 1097 . . 1103 1107 + TspDTI ATGAA 1118 1116 . . 1113 1117 + BsrI ACTGG 1118 1116 . . 1116 1121 + KpnI GGTACC 1120 1116 . . 1116 1121 + NlaIV GGNNCC 1118 1118 . . 1116 1121 + HgiCI* GGYRCC 1116 1120 . . 1116 1121 + Acc65I GGTACC 1116 1120 . . 1117 1120 + Csp6I GTAC 1117 1119 . . 1117 1120 + RsaI GTAC 1118 1118 . . 1120 1123 + AciI CCGC 1120 1122 . . 1122 1126 + SfaNI GCATC 1131 1135 . . 1123 1127 - FokI GGATG 1109 1113 . . 1123 1127 - BseGI GGATG 1122 1120 . . 1130 1133 + MseI TTAA 1130 1132 . . 1135 1139 + BssKI CCNGG 1134 1139 . . 1135 1139 + BseBI CCWGG 1136 1137 . . 1135 1139 + ScrFI CCNGG 1136 1137 . . 1135 1139 + EcoRII CCWGG 1134 1139 . . 1138 1142 + HphI GGTGA 1150 1149 . . 1140 1145 + BclI TGATCA 1140 1144 . . 1141 1144 + DpnI GATC 1142 1142 . . 1141 1144 + BstKTI GATC 1143 1141 . . 1141 1144 + MboI GATC 1140 1144 . . 1143 1148 + Hpy178III* TCNNGA 1144 1146 . . 1143 1148 + BspHI TCATGA 1143 1147 . . 1144 1147 + FatI CATG 1143 1147 . . 1144 1147 + NlaIII CATG 1147 1143 . . 1144 1147 + CviAII CATG 1144 1146 . . 1145 1149 + TspDTI ATGAA 1160 1158 . . 1156 1159 + TaqI TCGA 1156 1158 . . 1160 1164 + MaeIII GTNAC 1159 1164 . . 1168 1173 + Hpy166II GTNNAC 1170 1170 . . 1176 1180 + BinI* GGATC 1184 1185 . . 1177 1180 + DpnI GATC 1178 1178 . . 1177 1180 + BstKTI GATC 1179 1177 . . 1177 1180 + MboI GATC 1176 1180 . . 1179 1183 + Hpy188I TCNGA 1181 1180 . . 1185 1190 + AsuII TTCGAA 1186 1188 . . 1186 1189 + TaqI TCGA 1186 1188 . . 1190 1193 + SetI ASST 1193 1189 . . 1191 1194 + MnlI CCTC 1201 1200 . . 1196 1200 + BssKI CCNGG 1195 1200 . . 1196 1200 + BseBI CCWGG 1197 1198 . . 1196 1200 + ScrFI CCNGG 1197 1198 . . 1196 1200 + EcoRII CCWGG 1195 1200 . . 1203 1206 + CviRI* TGCA 1204 1204 . . 1211 1214 + MaeI CTAG 1211 1213 . . 1215 1219 + Hpy188I TCNGA 1217 1216 . . 1218 1223 + ApoI RAATTY 1218 1222 . . 1219 1222 + TspEI AATT 1218 1222 . . 1225 1229 + BccI CCATC 1233 1234 . . 1235 1238 + TaqI TCGA 1235 1237 . . 1237 1241 + TfiI GAWTC 1237 1240 . . 1237 1241 + HinfI GANTC 1237 1240 . . 1242 1245 + SetI ASST 1245 1241 . . 1242 1245 + CviJI RGCY 1243 1243 . . 1242 1245 + AluI AGCT 1243 1243 . . 1252 1257 + Hpy178III* TCNNGA 1253 1255 . . 1254 1264 + FalI AAGNNNNNCTT 1245 1240 1277 1272 1254 1264 - FalI AAGNNNNNCTT 1277 1272 1245 1240 1266 1272 + TspRI CASTGNN 1272 1263 . . 1268 1272 + Tsp45I GTSAC 1267 1272 . . 1268 1272 + MaeIII GTNAC 1267 1272 . . 1265 1269 - BsrI ACTGG 1265 1263 . . 1271 1275 + Tsp4CI* ACNGT 1273 1272 . . 1272 1282 + BsiYI* CCNNNNNNNGG 1278 1275 . . 1274 1279 - MmeI TCCRAC 1255 1253 . . 1278 1281 - MnlI CCTC 1271 1270 . . 1285 1289 - MboII GAAGA 1277 1276 . . 1284 1289 - BbvII* GAAGAC 1277 1281 . . 1290 1293 + TspEI AATT 1289 1293 . . 1297 1301 + MboII GAAGA 1309 1308 . . 1303 1307 - BsmAI GTCTC 1297 1301 . . 1313 1318 + Hpy178III* TCNNGA 1314 1316 . . 1326 1329 + FatI CATG 1325 1329 . . 1326 1329 + NlaIII CATG 1329 1325 . . 1326 1329 + CviAII CATG 1326 1328 . . 1328 1333 - TaqII CACCCA 1318 1316 . . 1334 1338 - TspDTI ATGAA 1324 1322 . . 1338 1342 + Hpy188I TCNGA 1340 1339 . . 1352 1356 + TspDTI ATGAA 1367 1365 . . 1353 1364 + BdaI TGANNNNNNTCA 1342 1340 1376 1374 1359 1364 + BclI TGATCA 1359 1363 . . 1360 1363 + DpnI GATC 1361 1361 . . 1360 1363 + BstKTI GATC 1362 1360 . . 1360 1363 + MboI GATC 1359 1363 . . 1363 1372 + MslI CAYNNNNRTG 1367 1367 . . 1364 1367 + SetI ASST 1367 1363 . . 1353 1364 - BdaI TGANNNNNNTCA 1376 1374 1342 1340 1362 1366 - HphI GGTGA 1354 1353 . . 1370 1374 + TspDTI ATGAA 1385 1383 . . 1379 1383 + TspDTI ATGAA 1394 1392 . . 1419 1422 + SetI ASST 1422 1418 . . 1420 1423 + MnlI CCTC 1430 1429 . . 1422 1426 + Hpy188I TCNGA 1424 1423 . . 1432 1436 + TfiI GAWTC 1432 1435 . . 1432 1436 + HinfI GANTC 1432 1435 . . 1438 1442 - TspGWI ACGGA 1428 1426 . . 1453 1462 + BsaBI GATNNNNATC 1457 1457 . . 1461 1465 + Hpy188I TCNGA 1463 1462 . . 1474 1478 + TspDTI ATGAA 1489 1487 . . 1476 1481 + EcoRI GAATTC 1476 1480 . . 1476 1481 + ApoI RAATTY 1476 1480 . . 1477 1480 + TspEI AATT 1476 1480 . . 1493 1498 + BspLU11I* ACATGT 1493 1497 . . 1493 1498 + NspI RCATGY 1497 1493 . . 1493 1498 + AflIII ACRYGT 1493 1497 . . 1494 1497 + FatI CATG 1493 1497 . . 1494 1497 + NlaIII CATG 1497 1493 . . 1494 1497 + CviAII CATG 1494 1496 . . 1497 1501 + Tsp45I GTSAC 1496 1501 . . 1497 1501 + MaeIII GTNAC 1496 1501 . . 1498 1502 - HphI GGTGA 1490 1489 . . 1504 1508 + MboII GAAGA 1516 1515 . . 1504 1509 + BbvII* GAAGAC 1511 1515 . . 1508 1511 + SetI ASST 1511 1507 . . 1510 1515 + SmlI CTYRAG 1510 1514 . . 1510 1515 + Bce83I* CTTGAG 1531 1529 . . 1513 1516 - MnlI CCTC 1506 1505 . . 1534 1539 + BsiI* CACGAG 1534 1538 . . 1546 1549 + TaqI TCGA 1546 1548 . . 1546 1551 + Hpy178III* TCNNGA 1547 1549 . . 1537 1547 - Hin4I GAYNNNNNVTC 1560 1555 1528 1523 1553 1556 + TspEI AATT 1552 1556 . . 1559 1563 + TspDTI ATGAA 1574 1572 . . 1572 1577 + ApoI RAATTY 1572 1576 . . 1573 1576 + TspEI AATT 1572 1576 . . 1585 1590 + Eco57MI CTGRAG 1606 1604 . . 1585 1590 + Eco57I CTGAAG 1606 1604 . . 1600 1603 + MaeI CTAG 1600 1602 . . 1608 1611 + TaqI TCGA 1608 1610 . . 1611 1614 + TspEI AATT 1610 1614 . . 1621 1625 + BsrI ACTGG 1626 1624 . . 1622 1627 + Eco57MI CTGRAG 1643 1641 . . 1622 1627 + GsuI CTGGAG 1643 1641 . . 1618 1622 - BsmAI GTCTC 1612 1616 . . 1656 1659 + CviRI* TGCA 1657 1657 . . 1670 1674 + TfiI GAWTC 1670 1673 . . 1670 1674 + HinfI GANTC 1670 1673 . . 1676 1681 + ApoI RAATTY 1676 1680 . . 1677 1680 + TspEI AATT 1676 1680 . . 1683 1687 + TauI GCSGC 1686 1683 . . 1683 1687 + BlsI GCNGC 1685 1684 . . 1683 1687 + BisI GCNGC 1684 1685 . . 1684 1687 + AciI CCGC 1684 1686 . . 1710 1713 + TspEI AATT 1709 1713 . . 1747 1751 + PleI GAGTC 1755 1756 . . 1747 1751 + MlyI GAGTC 1756 1756 . . 1747 1751 + HinfI GANTC 1747 1750 . . 1750 1753 + TaqI TCGA 1750 1752 . . 1747 1757 - Hin4I GAYNNNNNVTC 1770 1765 1738 1733 1762 1765 + TspEI AATT 1761 1765 . . 1769 1772 + MnlI CCTC 1779 1778 . . 1770 1775 + Ksp632I* CTCTTC 1776 1779 . . 1766 1770 - BslFI GGGAC 1751 1755 . . 1771 1775 - MboII GAAGA 1763 1762 . . 1773 1777 - TspDTI ATGAA 1763 1761 . . 1777 1781 + Hpy188I TCNGA 1779 1778 . . 1786 1790 + MaeIII GTNAC 1785 1790 . . 1787 1792 + TsoI TARCCA 1803 1801 . . 1793 1796 + CviJI RGCY 1794 1794 . . 1799 1802 + TaqI TCGA 1799 1801 . . 1801 1811 + Hin4I GAYNNNNNVTC 1792 1787 1824 1819 1804 1808 + TspDTI ATGAA 1819 1817 . . 1801 1811 - Hin4I GAYNNNNNVTC 1824 1819 1792 1787 1833 1836 + SetI ASST 1836 1832 . . 1831 1835 - Hin4II* CCTTC 1825 1824 . . 1840 1845 + Hpy166II GTNNAC 1842 1842 . . 1846 1850 - BccI CCATC 1840 1841 . . 1861 1865 + Hpy188I TCNGA 1863 1862 . . 1879 1883 - SfaNI GCATC 1869 1873 . . 1886 1890 + TspDTI ATGAA 1901 1899 . . 1888 1892 + MboII GAAGA 1900 1899 . . 1894 1904 + Hin4I GAYNNNNNVTC 1885 1880 1917 1912 1895 1899 + TspDTI ATGAA 1910 1908 . . 1894 1904 - Hin4I GAYNNNNNVTC 1917 1912 1885 1880 1906 1909 + MaeI CTAG 1906 1908 . . 1912 1915 + MseI TTAA 1912 1914 . . 1913 1918 + TsoI TARCCA 1929 1927 . . 1926 1930 + MboII GAAGA 1938 1937 . . 1929 1933 + MboII GAAGA 1941 1940 . . 1929 1934 - Ksp632I* CTCTTC 1924 1927 . . 1932 1935 - MnlI CCTC 1925 1924 . . 1941 1944 + SetI ASST 1944 1940 . . 1940 1943 - MnlI CCTC 1933 1932 . . 1945 1948 + TaqI TCGA 1945 1947 . . 1945 1950 + Hpy178III* TCNNGA 1946 1948 . . 1934 1946 - AloI GAACNNNNNNTCC 1958 1953 1926 1921 1949 1954 + BseRI GAGGAG 1964 1962 . . 1949 1952 - MnlI CCTC 1942 1941 . . 1952 1955 - MnlI CCTC 1945 1944 . . 1955 1965 + MwoI GCNNNNNNNGC 1961 1958 . . 1962 1966 + BmgT120I GGNCC 1963 1964 . . 1962 1966 + AsuI* GGNCC 1962 1965 . . 1963 1966 + CviJI RGCY 1964 1964 . . 1963 1966 + HaeIII GGCC 1964 1964 . . 1980 1983 + CviRI* TGCA 1981 1981 . . 1977 1982 - BsrDI GCAATG 1976 1974 . . 2007 2012 + AhaIII* TTTAAA 2009 2009 . . 2008 2011 + MseI TTAA 2008 2010 . . 2013 2018 + ApoI RAATTY 2013 2017 . . 2014 2017 + TspEI AATT 2013 2017 . . 2023 2028 + BciVI GTATCC 2034 2033 . . 2026 2030 + Hpy188I TCNGA 2028 2027 . . 2033 2038 + ApoI RAATTY 2033 2037 . . 2034 2037 + TspEI AATT 2033 2037 . . 2040 2043 + SetI ASST 2043 2039 . . 2042 2046 + MaeIII GTNAC 2041 2046 . . 2045 2049 + Tsp4CI* ACNGT 2047 2046 . . 2049 2053 - TspDTI ATGAA 2039 2037 . . 2053 2058 + Hpy178III* TCNNGA 2054 2056 . . 2059 2063 + Tsp4CI* ACNGT 2061 2060 . . 2087 2091 + MboII GAAGA 2099 2098 . . 2087 2092 + BbvII* GAAGAC 2094 2098 . . 2091 2094 + SetI ASST 2094 2090 . . 2098 2101 - AciI CCGC 2098 2100 . . 2113 2116 + MseI TTAA 2113 2115 . . 2115 2118 + TspEI AATT 2114 2118 . . 2126 2130 + Tsp45I GTSAC 2125 2130 . . 2126 2130 + MaeIII GTNAC 2125 2130 . . 2133 2136 + SetI ASST 2136 2132 . . 2133 2136 + CviJI RGCY 2134 2134 . . 2133 2136 + AluI AGCT 2134 2134 . . 2137 2140 + MseI TTAA 2137 2139 . . 2165 2170 + BtgZI GCGATG 2180 2184 . . 2169 2174 + BclI TGATCA 2169 2173 . . 2170 2173 + DpnI GATC 2171 2171 . . 2170 2173 + BstKTI GATC 2172 2170 . . 2170 2173 + MboI GATC 2169 2173 . . 2172 2176 - HphI GGTGA 2164 2163 . . 2180 2183 + TaiI ACGT 2183 2179 . . 2180 2183 + SetI ASST 2183 2179 . . 2180 2183 + MaeII ACGT 2180 2182 . . 2195 2199 + Tsp4CI* ACNGT 2197 2196 . . 2189 2199 - BaeI ACNNNNGTAYC 2214 2209 2181 2176 2200 2204 + Hpy188I TCNGA 2202 2201 . . 2236 2241 + SacI GAGCTC 2240 2236 . . 2236 2241 + Ecl136II GAGCTC 2238 2238 . . 2236 2241 + SduI GDGCHC 2240 2236 . . 2236 2241 + HgiJII* GRGCYC 2240 2236 . . 2236 2241 + HgiAI* GWGCWC 2240 2236 . . 2237 2240 + SetI ASST 2240 2236 . . 2237 2240 + CviJI RGCY 2238 2238 . . 2237 2240 + AluI AGCT 2238 2238 . . 2242 2245 + CviRI* TGCA 2243 2243 . . 2245 2248 + TspEI AATT 2244 2248 . . 2252 2255 + SetI ASST 2255 2251 . . 2253 2257 + Hin4II* CCTTC 2263 2262 . . 2256 2261 + Hpy178III* TCNNGA 2257 2259 . . 2269 2273 + PleI GAGTC 2277 2278 . . 2269 2273 + MlyI GAGTC 2278 2278 . . 2269 2273 + HinfI GANTC 2269 2272 . . 2273 2277 + DdeI CTNAG 2273 2276 . . 2284 2288 + Hpy188I TCNGA 2286 2285 . . 2288 2292 + TspDTI ATGAA 2303 2301 . . 2294 2297 + SetI ASST 2297 2293 . . 2300 2303 + TspEI AATT 2299 2303 . . 2322 2325 + TspEI AATT 2321 2325 . . 2334 2338 - BsmAI GTCTC 2328 2332 . . 2344 2348 - HgaI GACGC 2333 2338 . . 2348 2352 + BssKI CCNGG 2347 2352 . . 2348 2351 + HpaII CCGG 2348 2350 . . 2348 2353 + SecI* CCNNGG 2348 2352 . . 2348 2352 + ScrFI CCNGG 2349 2350 . . 2348 2352 + CauII* CCSGG 2349 2350 . . 2370 2375 + AhaIII* TTTAAA 2372 2372 . . 2371 2374 + MseI TTAA 2371 2373 . . 2376 2386 + Hin4I GAYNNNNNVTC 2367 2362 2399 2394 2382 2386 + BccI CCATC 2390 2391 . . 2385 2388 + TaqI TCGA 2385 2387 . . 2385 2390 + Hpy178III* TCNNGA 2386 2388 . . 2376 2386 - Hin4I GAYNNNNNVTC 2399 2394 2367 2362 2391 2396 + AhaIII* TTTAAA 2393 2393 . . 2392 2395 + MseI TTAA 2392 2394 . . 2396 2399 + CviJI RGCY 2397 2397 . . 2398 2401 + MnlI CCTC 2408 2407 . . 2404 2409 + SspI AATATT 2406 2406 . . 2422 2425 + TaqI TCGA 2422 2424 . . 2428 2431 + SetI ASST 2431 2427 . . 2430 2435 + Hpy166II GTNNAC 2432 2432 . . 2440 2443 + DpnI GATC 2441 2441 . . 2440 2443 + BstKTI GATC 2442 2440 . . 2440 2443 + MboI GATC 2439 2443 . . 2458 2467 + XmnI GAANNNNTTC 2462 2462 . . 2465 2470 + AsuII TTCGAA 2466 2468 . . 2466 2469 + TaqI TCGA 2466 2468 . . 2463 2467 - MboII GAAGA 2455 2454 . . 2468 2473 + ApoI RAATTY 2468 2472 . . 2469 2472 + TspEI AATT 2468 2472 . . 2475 2480 + EcoRV GATATC 2477 2477 . . 2479 2483 + Hpy188I TCNGA 2481 2480 . . 2482 2491 + PshAI GACNNNNGTC 2486 2486 . . 2494 2497 + CviRI* TGCA 2495 2495 . . 2503 2506 + MaeI CTAG 2503 2505 . . 2506 2510 + HinfI GANTC 2506 2509 . . 2506 2515 + PshAI GACNNNNGTC 2510 2510 . . 2506 2510 - PleI GAGTC 2500 2501 . . 2506 2510 - MlyI GAGTC 2500 2500 . . 2518 2522 - MboII GAAGA 2510 2509 . . 2517 2522 - BbvII* GAAGAC 2510 2514 . . 2520 2524 - TspDTI ATGAA 2510 2508 . . 2532 2537 + ApoI RAATTY 2532 2536 . . 2533 2536 + TspEI AATT 2532 2536 . . 2545 2549 + Hin4II* CCTTC 2555 2554 . . 2561 2566 - MmeI TCCRAC 2542 2540 . . 2566 2572 + DraII RGGNCCY 2567 2570 . . 2567 2573 + DraII RGGNCCY 2568 2571 . . 2567 2572 + SduI GDGCHC 2571 2567 . . 2567 2572 + Bsp120I GGGCCC 2567 2571 . . 2567 2572 + NlaIV GGNNCC 2569 2569 . . 2567 2571 + BmgT120I GGNCC 2568 2569 . . 2567 2572 + HgiJII* GRGCYC 2571 2567 . . 2567 2572 + BseSI GKGCMC 2571 2567 . . 2567 2571 + AsuI* GGNCC 2567 2570 . . 2567 2572 + ApaI GGGCCC 2571 2567 . . 2565 2568 - MnlI CCTC 2558 2557 . . 2568 2571 + CviJI RGCY 2569 2569 . . 2568 2571 + HaeIII GGCC 2569 2569 . . 2568 2573 + NlaIV GGNNCC 2570 2570 . . 2568 2572 + BmgT120I GGNCC 2569 2570 . . 2568 2572 + AsuI* GGNCC 2568 2571 . . 2576 2579 + SetI ASST 2579 2575 . . 2576 2579 + CviJI RGCY 2577 2577 . . 2576 2579 + AluI AGCT 2577 2577 . . 2577 2581 + BlsI GCNGC 2579 2578 . . 2577 2581 + BisI GCNGC 2578 2579 . . 2577 2581 + TseI GCWGC 2577 2580 . . 2577 2581 - BbvI GCAGC 2564 2568 . . 2584 2588 + MboII GAAGA 2596 2595 . . 2587 2591 + TfiI GAWTC 2587 2590 . . 2587 2591 + HinfI GANTC 2587 2590 . . 2596 2599 + TspEI AATT 2595 2599 . . 2600 2603 + CviRI* TGCA 2601 2601 . . 2601 2606 + BtsI GCAGTG 2608 2606 . . 2602 2608 + TspRI CASTGNN 2608 2599 . . 2612 2615 + TaqI TCGA 2612 2614 . . 2617 2621 + TspGWI ACGGA 2632 2630 . . 2625 2630 + Ksp632I* CTCTTC 2631 2634 . . 2626 2630 - MboII GAAGA 2618 2617 . . 2630 2633 + MaeI CTAG 2630 2632 . . 2641 2645 + Tsp4CI* ACNGT 2643 2642 . . 2649 2653 - TspDTI ATGAA 2639 2637 . . 2653 2657 + Hpy188I TCNGA 2655 2654 . . 2656 2660 + TfiI GAWTC 2656 2659 . . 2656 2660 + HinfI GANTC 2656 2659 . . 2668 2671 + DpnI GATC 2669 2669 . . 2668 2671 + BstKTI GATC 2670 2668 . . 2668 2671 + MboI GATC 2667 2671 . . 2668 2672 - BinI* GGATC 2662 2663 . . 2673 2678 + Hpy166II GTNNAC 2675 2675 . . 2670 2674 - TspGWI ACGGA 2660 2658 . . 2678 2688 + BsiYI* CCNNNNNNNGG 2684 2681 . . 2675 2679 - HphI GGTGA 2667 2666 . . 2682 2687 + BseRI GAGGAG 2697 2695 . . 2682 2685 - MnlI CCTC 2675 2674 . . 2687 2690 + CviJI RGCY 2688 2688 . . 2685 2688 - MnlI CCTC 2678 2677 . . 2695 2700 + XhoII RGATCY 2695 2699 . . 2695 2700 + BglII AGATCT 2695 2699 . . 2696 2699 + DpnI GATC 2697 2697 . . 2696 2699 + BstKTI GATC 2698 2696 . . 2696 2699 + MboI GATC 2695 2699 . . 2719 2723 + FokI GGATG 2732 2736 . . 2719 2723 + BseGI GGATG 2725 2723 . . 2754 2758 + SfaNI GCATC 2763 2767 . . 2755 2759 - FokI GGATG 2741 2745 . . 2755 2759 - BseGI GGATG 2754 2752 . . 2771 2776 + SspI AATATT 2773 2773 . . 2777 2782 + BsaAI YACGTR 2779 2779 . . 2777 2782 + PmaCI CACGTG 2779 2779 . . 2778 2781 + TaiI ACGT 2781 2777 . . 2778 2781 + SetI ASST 2781 2777 . . 2778 2781 + MaeII ACGT 2778 2780 . . 2798 2801 - MnlI CCTC 2791 2790 . . 2802 2807 + SspI AATATT 2804 2804 . . 2811 2816 + Hpy178III* TCNNGA 2812 2814 . . 2816 2820 + TspDTI ATGAA 2831 2829 . . 2821 2825 + TspDTI ATGAA 2836 2834 . . 2828 2833 + Hpy166II GTNNAC 2830 2830 . . 2828 2833 + AccI GTMKAC 2829 2831 . . 2833 2836 + FatI CATG 2832 2836 . . 2833 2836 + NlaIII CATG 2836 2832 . . 2833 2836 + CviAII CATG 2833 2835 . . 2839 2844 + XhoII RGATCY 2839 2843 . . 2840 2843 + DpnI GATC 2841 2841 . . 2840 2843 + BstKTI GATC 2842 2840 . . 2840 2843 + MboI GATC 2839 2843 . . 2840 2844 - BinI* GGATC 2834 2835 . . 2845 2848 + MseI TTAA 2845 2847 . . 2847 2850 + TspEI AATT 2846 2850 . . 2850 2853 + CviRI* TGCA 2851 2851 . . 2856 2859 + SetI ASST 2859 2855 . . 2856 2859 + CviJI RGCY 2857 2857 . . 2856 2859 + AluI AGCT 2857 2857 . . 2858 2863 + SfeI* CTRYAG 2858 2862 . . 2862 2867 + XhoII RGATCY 2862 2866 . . 2862 2867 + BglII AGATCT 2862 2866 . . 2863 2873 + Hin4I GAYNNNNNVTC 2854 2849 2886 2881 2863 2866 + DpnI GATC 2864 2864 . . 2863 2866 + BstKTI GATC 2865 2863 . . 2863 2866 + MboI GATC 2862 2866 . . 2865 2869 + Hpy188I TCNGA 2867 2866 . . 2868 2871 + DpnI GATC 2869 2869 . . 2868 2871 + BstKTI GATC 2870 2868 . . 2868 2871 + MboI GATC 2867 2871 . . 2883 2888 + BclI TGATCA 2883 2887 . . 2884 2887 + DpnI GATC 2885 2885 . . 2884 2887 + BstKTI GATC 2886 2884 . . 2884 2893 + BsaBI GATNNNNATC 2888 2888 . . 2884 2887 + MboI GATC 2883 2887 . . 2886 2891 + Hpy178III* TCNNGA 2887 2889 . . 2890 2893 + DpnI GATC 2891 2891 . . 2890 2893 + BstKTI GATC 2892 2890 . . 2890 2893 + MboI GATC 2889 2893 . . 2890 2894 - BinI* GGATC 2884 2885 . . 2892 2896 - TspGWI ACGGA 2882 2880 . . 2896 2899 + MseI TTAA 2896 2898 . . 2904 2907 + SetI ASST 2907 2903 . . 2914 2918 + TspDTI ATGAA 2929 2927 . . 2923 2927 + DdeI CTNAG 2923 2926 . . 2928 2932 + SfaNI GCATC 2937 2941 . . 2929 2933 - FokI GGATG 2915 2919 . . 2929 2933 - BseGI GGATG 2928 2926 . . 2931 2935 - TspGWI ACGGA 2921 2919 . . 2941 2946 + BalI TGGCCA 2943 2943 . . 2941 2946 + CfrI YGGCCR 2941 2945 . . 2942 2945 + CviJI RGCY 2943 2943 . . 2942 2945 + HaeIII GGCC 2943 2943 . . 2950 2953 + TaqI TCGA 2950 2952 . . 2957 2960 + TspEI AATT 2956 2960 . . 2970 2975 + AhaIII* TTTAAA 2972 2972 . . 2971 2974 + MseI TTAA 2971 2973 . . 2987 2990 + CviJI RGCY 2988 2988 . . 2994 2997 + TspEI AATT 2993 2997 . . 3005 3009 - BsmAI GTCTC 2999 3003 . . 3010 3013 + MnlI CCTC 3020 3019 . . 3005 3010 - Eco31I GGTCTC 2999 3003 . . 3017 3022 + SduI GDGCHC 3021 3017 . . 3017 3022 + BseSI GKGCMC 3021 3017 . . 3025 3028 + MseI TTAA 3025 3027 . . 3028 3032 + TspDTI ATGAA 3043 3041 . . 3031 3036 + ApoI RAATTY 3031 3035 . . 3032 3035 + TspEI AATT 3031 3035 . . 3037 3041 + HgaI GACGC 3046 3051 . . 3037 3042 + AcyI GRCGYC 3038 3040 . . 3040 3045 + Cfr10I RCCGGY 3040 3044 . . 3041 3044 + HpaII CCGG 3041 3043 . . 3046 3050 + Hpy188I TCNGA 3048 3047 . . 3063 3069 + TspRI CASTGNN 3069 3060 . . 3062 3066 - BsrI ACTGG 3062 3060 . . 3061 3066 - BfiI ACTGGG 3056 3055 . . 3076 3080 + Tsp4CI* ACNGT 3078 3077 . . 3099 3102 + FatI CATG 3098 3102 . . 3099 3102 + NlaIII CATG 3102 3098 . . 3099 3102 + CviAII CATG 3099 3101 . . 3097 3101 - TspDTI ATGAA 3087 3085 . . 3107 3110 + SetI ASST 3110 3106 . . 3115 3118 + SetI ASST 3118 3114 . . 3117 3120 + Csp6I GTAC 3117 3119 . . 3117 3120 + RsaI GTAC 3118 3118 . . 3132 3136 - TspDTI ATGAA 3122 3120 . . 3142 3145 + MseI TTAA 3142 3144 . . 3147 3152 + XhoII RGATCY 3147 3151 . . 3147 3151 + BinI* GGATC 3155 3156 . . 3148 3151 + DpnI GATC 3149 3149 . . 3148 3151 + BstKTI GATC 3150 3148 . . 3148 3151 + MboI GATC 3147 3151 . . 3154 3157 + MseI TTAA 3154 3156 . . 3158 3163 + SduI GDGCHC 3162 3158 . . 3158 3163 + HgiAI* GWGCWC 3162 3158 . . 3163 3167 + DdeI CTNAG 3163 3166 . . 3195 3198 + SetI ASST 3198 3194 . . 3197 3202 + Eco57MI CTGRAG 3218 3216 . . 3197 3202 + Eco57I CTGAAG 3218 3216 . . 3199 3203 + MboII GAAGA 3211 3210 . . 3219 3224 + NlaIV GGNNCC 3221 3221 . . 3220 3224 + BmgT120I GGNCC 3221 3222 . . 3220 3224 + AsuI* GGNCC 3220 3223 . . 3221 3224 + CviJI RGCY 3222 3222 . . 3221 3224 + HaeIII GGCC 3222 3222 . . 3222 3227 + Cfr10I RCCGGY 3222 3226 . . 3223 3226 + HpaII CCGG 3223 3225 . . 3225 3230 + KpnI GGTACC 3229 3225 . . 3225 3230 + NlaIV GGNNCC 3227 3227 . . 3225 3230 + HgiCI* GGYRCC 3225 3229 . . 3225 3230 + Acc65I GGTACC 3225 3229 . . 3226 3229 + Csp6I GTAC 3226 3228 . . 3226 3229 + RsaI GTAC 3227 3227 . . 3232 3236 - BccI CCATC 3226 3227 . . 3251 3255 - HphI GGTGA 3243 3242 . . 3269 3272 + SetI ASST 3272 3268 . . 3273 3278 + VspI ATTAAT 3274 3276 . . 3274 3277 + MseI TTAA 3274 3276 . . 3279 3282 + SetI ASST 3282 3278 . . 3303 3308 + ApoI RAATTY 3303 3307 . . 3304 3307 + TspEI AATT 3303 3307 . . 3307 3310 + MseI TTAA 3307 3309 . . 3311 3315 + Tsp45I GTSAC 3310 3315 . . 3311 3315 + MaeIII GTNAC 3310 3315 . . 3316 3319 + DpnI GATC 3317 3317 . . 3316 3319 + BstKTI GATC 3318 3316 . . 3316 3319 + MboI GATC 3315 3319 . . 3320 3325 + ApoI RAATTY 3320 3324 . . 3321 3324 + TspEI AATT 3320 3324 . . 3326 3331 + BsaAI YACGTR 3328 3328 . . 3327 3330 + TaiI ACGT 3330 3326 . . 3327 3330 + SetI ASST 3330 3326 . . 3327 3330 + MaeII ACGT 3327 3329 . . 3331 3336 + ApoI RAATTY 3331 3335 . . 3332 3335 + TspEI AATT 3331 3335 . . 3342 3346 - TspDTI ATGAA 3332 3330 . . #--------------------------------------- # # # Acc65I: # [GGTACC] # 123 1116 2109 # # AccI: # [GTMKAC] # 6 29 519 665 2129 # # AciI: # [CCGC] # 25 102 414 488 505 564 # 1250 # # AclI: # [AACGTT] # 502 2846 # # AcyI: # [GRCGYC] # 310 3038 # # AflIII: # [ACRYGT] # 20 690 783 1855 # # AhaIII*: # [TTTAAA] # 21 363 376 579 2009 # # AloI: # [GAACNNNNNNTCC] # 32 1390 1926 # # AluI: # [AGCT] # 29 89 104 141 280 339 # 356 491 628 891 # # ApaI: # [GGGCCC] # 777 2571 # # ApoI: # [RAATTY] # 11 17 17 20 64 96 # 104 258 272 337 355 416 # 435 447 499 # # AsuI*: # [GGNCC] # 1 128 605 652 742 1220 # # AsuII: # [TTCGAA] # 12 882 1174 1280 # # AvaII: # [GGWCC] # 742 2606 # # BaeI: # [ACNNNNGTAYC] # 33 1134 2181 # # BalI: # [TGGCCA] # 405 2943 # # BbvI: # [GCAGC] # 100 211 285 328 784 1640 # # BbvII*: # [GAAGAC] # 234 416 583 838 1277 # # BccI: # [CCATC] # 40 67 122 157 205 311 # 453 550 607 836 # # Bce83I*: # [CTTGAG] # 81 1450 1817 # # BceAI: # [ACGGC] # 981 2367 # # BciVI: # [GTATCC] # 1314 2034 # # BclI: # [TGATCA] # 219 465 714 810 1140 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 1342 1972 # # BfiI: # [ACTGGG] # 292 3056 # # BglII: # [AGATCT] # 167 486 2695 # # BinI*: # [GGATC] # 50 172 193 271 497 687 # 1478 # # BisI: # [GCNGC] # 75 103 196 236 303 770 # 771 894 # # BlsI: # [GCNGC] # 75 104 196 236 303 769 # 771 894 # # BmgT120I: # [GGNCC] # 1 127 605 652 743 1220 # # BmtI: # [GCTAGC] # 1006 2342 # # BsaAI: # [YACGTR] # 20 549 2779 # # BsaBI: # [GATNNNNATC] # 460 1431 1457 # # BsaXI: # [ACNNNNNCTCC] # 30 762 2556 # # BseBI: # [CCWGG] # 61 390 746 2151 # # BseGI: # [GGATG] # 29 55 174 420 1067 1603 # # BseMII: # [CTCAG] # 100 240 445 2563 # # BseRI: # [GAGGAG] # 651 733 811 1153 # # BseSI: # [GKGCMC] # 327 450 2571 # # BseYI: # [CCCAGC] # 858 2490 # # BsgI: # [GTGCAG] # 595 2753 # # BsiI*: # [CACGAG] # 1534 1814 # # BsiYI*: # [CCNNNNNNNGG] # 101 223 456 498 664 1406 # # BslFI: # [GGGAC] # 1597 1751 # # BsmAI: # [GTCTC] # 92 315 349 671 716 1205 # # Bsp120I: # [GGGCCC] # 781 2567 # # BspCNI: # [CTCAG] # 102 239 445 2562 # # BspHI: # [TCATGA] # 1143 2205 # # BspLU11I*: # [ACATGT] # 20 690 783 1855 # # BsrDI: # [GCAATG] # 979 997 1372 # # BsrI: # [ACTGG] # 147 286 361 437 681 1436 # # BssKI: # [CCNGG] # 61 81 309 744 1001 1152 # # BssNAI: # [GTATAC] # 701 2647 # # BstKTI: # [GATC] # 5 6 16 23 28 30 # 36 51 144 168 183 228 # 258 270 410 682 810 # # BtgZI: # [GCGATG] # 1168 2180 # # BtrI: # [CACGTC] # 274 3074 # # BtsI: # [GCAGTG] # 34 740 2574 # # Cac8I: # [GCNNGC] # 4 119 885 2340 # # CauII*: # [CCSGG] # 999 1055 1294 # # Cfr10I: # [RCCGGY] # 126 182 3040 # # CfrI: # [YGGCCR] # 407 2941 # # ClaI: # [ATCGAT] # 682 2666 # # Csp6I: # [GTAC] # 109 122 316 801 2000 # # CspCI: # [CAANNNNNGTGG] # 35 611 2702 # # CviAII: # [CATG] # 21 47 61 168 175 182 # 197 249 266 314 329 1339 # # CviJI: # [RGCY] # 8 29 45 68 78 85 # 86 89 104 111 126 141 # 159 169 170 170 172 172 # 184 234 397 551 # # CviRI*: # [TGCA] # 106 230 250 252 262 324 # 362 453 497 612 # # DdeI: # [CTNAG] # 123 185 226 240 445 650 # 1479 # # DpnI: # [GATC] # 5 6 16 23 28 31 # 36 51 144 168 183 228 # 258 270 410 681 810 # # DraII: # [RGGNCCY] # 1 780 2567 # # Ecl136II: # [GAGCTC] # 1110 2238 # # Eco31I: # [GGTCTC] # 349 2999 # # Eco47III: # [AGCGCT] # 941 2407 # # Eco57I: # [CTGAAG] # 130 132 224 572 678 1612 # # Eco57MI: # [CTGRAG] # 37 130 132 224 572 678 # 1575 # # EcoP15I: # [CAGCAG] # 232 3116 # # EcoRI: # [GAATTC] # 613 863 1872 # # EcoRII: # [CCWGG] # 61 390 744 2153 # # EcoRV: # [GATATC] # 871 2477 # # Esp3I: # [CGTCTC] # 92 3256 # # FalI: # [AAGNNNNNCTT] # 0 0 32 1245 2071 # # FatI: # [CATG] # 20 47 61 168 175 182 # 197 250 266 314 329 1339 # # FauI: # [CCCGC] # 614 2734 # # FokI: # [GGATG] # 9 42 174 433 1067 1623 # # GlaI: # [GCGC] # 39 941 2368 # # GsaI: # [CCCAGC] # 862 2486 # # GsuI: # [CTGGAG] # 1643 1705 # # HaeII: # [RGCGCY] # 39 943 2366 # # HaeIII: # [GGCC] # 126 279 374 605 1964 # # HgaI: # [GACGC] # 302 713 2333 # # HgiAI*: # [GWGCWC] # 186 922 2240 # # HgiCI*: # [GGYRCC] # 123 1116 2109 # # HgiJII*: # [GRGCYC] # 331 777 2240 # # HhaI: # [GCGC] # 39 942 2367 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 0 0 # 22 32 32 32 32 32 # 32 61 178 450 455 462 # 1528 # # Hin4II*: # [CCTTC] # 199 289 292 438 793 1337 # # Hin6I: # [GCGC] # 39 940 2369 # # HinfI: # [GANTC] # 69 77 81 195 237 238 # 310 330 522 597 692 # # HpaII: # [CCGG] # 125 182 693 1055 1293 # # HphI: # [GGTGA] # 49 105 136 198 204 368 # 503 535 576 674 # # Hpy166II: # [GTNNAC] # 7 29 75 105 155 204 # 243 281 469 518 590 672 # # Hpy178III*: # [TCNNGA] # 46 61 75 108 109 124 # 129 203 233 330 399 426 # 461 644 # # Hpy188I: # [TCNGA] # 36 39 84 84 84 99 # 101 123 135 165 174 174 # 181 195 205 212 281 300 # 316 360 # # Hpy99I: # [CGWCG] # 107 275 2966 # # KpnI: # [GGTACC] # 119 1120 2109 # # Ksp632I*: # [CTCTTC] # 148 707 717 1776 # # MaeI: # [CTAG] # 28 66 127 180 306 389 # 597 718 937 # # MaeII: # [ACGT] # 18 21 89 122 273 549 # 598 1678 # # MaeIII: # [GTNAC] # 38 84 108 229 256 265 # 289 334 560 1185 # # MboI: # [GATC] # 5 6 16 23 28 33 # 36 51 144 168 183 228 # 258 270 410 679 810 # # MboII: # [GAAGA] # 3 22 26 32 38 55 # 86 101 137 137 143 158 # 207 247 248 295 356 464 # 593 # # MlyI: # [GAGTC] # 222 522 634 848 1122 # # MmeI: # [TCCRAC] # 186 262 806 807 1287 # # MnlI: # [CCTC] # 3 3 8 9 9 33 # 70 76 89 105 113 117 # 146 150 159 229 240 273 # 313 328 412 463 # # MseI: # [TTAA] # 12 21 24 33 41 45 # 51 54 75 90 96 105 # 117 120 234 450 453 545 # 782 # # MslI: # [CAYNNNNRTG] # 495 872 1981 # # MwoI: # [GCNNNNNNNGC] # 1387 1961 # # NdeI: # [CATATG] # 68 427 2853 # # NheI: # [GCTAGC] # 1002 2346 # # NlaIII: # [CATG] # 24 47 61 168 175 182 # 197 246 266 314 329 1339 # # NlaIV: # [GGNNCC] # 1 6 121 346 651 772 # 1451 # # NspBII*: # [CMGCKG] # 609 2739 # # NspI: # [RCATGY] # 24 258 525 690 1851 # # PflMI: # [CCANNNNNTGG] # 780 2568 # # PleI: # [GAGTC] # 223 522 634 848 1121 # # PmaCI: # [CACGTG] # 569 2779 # # PshAI: # [GACNNNNGTC] # 24 838 2486 # # PsrI: # [GAACNNNNNNTAC] # 32 523 2793 # # RsaI: # [GTAC] # 109 121 317 801 2000 # # SacI: # [GAGCTC] # 1108 2240 # # SauI*: # [CCTNAGG] # 794 2554 # # ScrFI: # [CCNGG] # 61 81 309 746 999 1152 # # SduI: # [GDGCHC] # 141 186 331 450 2240 # # SecI*: # [CCNNGG] # 1000 1053 1295 # # SetI: # [ASST] # 7 8 10 14 15 18 # 18 18 21 22 29 42 # 42 44 46 47 48 48 # 48 51 52 55 57 65 # 67 74 76 76 78 80 # 89 89 97 99 108 122 # 134 148 152 202 203 304 # 325 # # SexAI: # [ACCWGGT] # 744 2604 # # SfaNI: # [GCATC] # 174 411 738 894 1131 # # SfeI*: # [CTRYAG] # 401 490 2457 # # SmlI: # [CTYRAG] # 96 1414 1838 # # SspI: # [AATATT] # 31 367 544 627 1779 # # TaiI: # [ACGT] # 18 18 89 122 276 549 # 598 1678 # # TaqI: # [TCGA] # 12 30 37 44 44 49 # 49 62 80 142 146 146 # 167 201 258 311 338 394 # 398 440 # # TaqII: # [CACCCA] # 1318 2030 # # TauI: # [GCSGC] # 105 1581 1662 # # TfiI: # [GAWTC] # 69 195 238 330 692 907 # 917 # # TseI: # [GCWGC] # 75 236 298 303 771 1665 # # TsoI: # [TARCCA] # 126 622 1181 1419 # # Tsp45I: # [GTSAC] # 38 229 373 629 894 1185 # # Tsp4CI*: # [ACNGT] # 14 136 270 435 446 774 # 1273 # # TspDTI: # [ATGAA] # 5 9 9 12 16 18 # 35 42 43 44 44 56 # 78 82 85 93 95 101 # 114 127 129 129 150 164 # 165 189 192 207 210 264 # 441 # # TspEI: # [AATT] # 11 17 17 20 20 22 # 33 33 37 38 38 52 # 55 63 64 66 71 75 # 76 81 110 130 138 144 # 144 147 159 160 162 187 # 203 251 252 272 # # TspGWI: # [ACGGA] # 28 39 222 427 452 976 # 1204 # # TspRI: # [CASTGNN] # 41 166 279 461 474 591 # 1336 # # TstI: # [CACNNNNNNTCC] # 32 32 161 762 2361 # # VspI: # [ATTAAT] # 74 3274 # # XhoII: # [RGATCY] # 23 144 201 285 2695 # # XmnI: # [GAANNNNTTC] # 449 886 2013 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 3348 # Reported_sequences: 1 # Reported_hitcount: 771 #---------------------------------------